CN100492004C - Pollutant aerobic biological degradation breath measuring method and device - Google Patents

Abstract

The invention is a pollutant aerobic-biodegradation breathing measuring method and device, relating to a method and device for measuring oxygen utilization ratio in the course of processing waste water by aerobes. And the measuring method comprises the steps of: checking dissolved oxygen sensor, keeping temperature of water tank constant, putting in microbial mixed solution, blending, circulating mixed solution, setting parameters, putting in to-be-measured sample and calculating; and the measuring device comprises water tank, magnetic blending mechanism, aeration mechanism, aeration chamber, breathing chamber, measuring chamber, pH sensor, dissolved oxygen sensor, signal transmit lines, transducer terminal box, computer and oxygen utilization ratio measuring software. And the invention has advantages of less interference, high testing accuracy and frequency, high instrument automation degree, etc. and it is applied to the aspects of city sewage composition characterization, inlet water toxicity detection, evaluation and early- warning in sewage factory, process control and operation management in sewage factory, etc.

Description

Pollutant aerobic biological degradation breath measuring method and device

Technical field

The present invention relates to a kind of pollutant aerobic biological degradation breath measuring method and device, specifically be that the oxygen of measuring pollutant in the active sludge microorganism aerobic degradation sewage utilizes speed (Oxygen Utilization/Uptake Rate, be called for short OUR), aspects such as it is applicable to that the municipal effluent component characterizes, detection, toxic action assessment and early warning, municipal sewage plant's process control and the operational management of sewage treatment plant inflow toxicant and the chamber research of wastewater treatment activated sludge process dynamic experiment belong to technical field of sewage.

Background technology

In the prior art, common breath measuring method and device, two standards of main foundation are divided: (1) measurement liquid phase still is the oxygen concentration in the gas phase; (2) whether the input and output (dynamically still static) of liquid or gas are arranged.The gas phase measuring principle is that oxygen concentration changes the oxygen consumption situation that reflects active sludge microorganism indirectly in the gas phase by measuring.What use this principle mainly is the electrolyte respirometer, as the BI-2000 type electrolyte respirometer of Merit20 type respiration monitoring instrument and Bioscience company.These respirometers are generally by reactor, electrolysis cells, CO ₂Catcher and related software are formed.Active sludge mixes in airtight reactor with water sample, biochemical reaction takes place consume oxygen generation CO ₂, CO ₂The catcher that KOH solution is housed absorbs the device internal gas pressure that induces reaction and descends, and pressure transducer detects this variation and connects the power supply of electrolysis cells, and electrolysis produces oxygen and replenishes the oxygen that is consumed, and keeps reaction bulb air pressure.Can calculate the amount of oxygen of generation by the metering current amount, just can obtain the total BOD and the OUR change curve of sample.This class respirometer generally need the sampling interval of a few minutes at least, and the microorganism of liquid internal can be subjected to the restriction of low oxygen concentration because the restriction of self ultimate principle makes it can not have too high test frequency.Thereby it only is applicable to the measurement of long-term total BOD, measures and be not suitable for dynamic process.

The respirometer of liquid phase principle has three kinds of principal modes: static gas phase-static liquid phase respirometer, the gas phase-static liquid phase respirometer that flows, static gas phase-mobile liquid phase respirometer.

It is static that gas phase-static liquid phase is meant at the duration of test reactive system and the external world does not have mass exchange.The most typical representative of this respiration measurement technology is exactly batch formula OUR analyzer of using always, measures dissolved oxygen concentration decline in time in airtight reactor.Do not have extraneous oxygen transmission to make the simple in structure of this respirometer, but exist since the interior dissolved oxygen concentration of reactor to cross the low microbial activity that causes limited.

Gas phase-static liquid phase the respirometer that flows is meant oxygenation in reaction system continuously, RODTOX (Rapid Oxygen Demand and TOXicity tester) respiration measurement instrument as the Belgian Gent MicrobialEcology of university development in laboratory, this respirometer need be estimated oxygen mass transfer coefficients KLa, cause experimental error easily, cause the measuring accuracy of OUR low.

Static gas phase-liquid phase the respirometer that flows, it is the airtight excessively respiratory reaction device of a kind of aeration mixed liquor continuous stream, measures the dissolved oxygen concentration of import and outlet.As the RA-1000 respiration measurement instrument of Environmental Technology system of Dutch Wageningen agriculture university exploitation, this respirometer uses same sensor measurement import and export mixed liquor dissolved oxygen concentration by changing to flow to, thereby has also limited the test frequency of OUR.

Defectives such as above-mentioned breath measuring method and device all exist measuring accuracy not high in practice, and test frequency is lower.

Summary of the invention

The purpose of this invention is to provide a kind of pollutant aerobic biological degradation breath measuring method and device, realize the respiration measurement of high precision, high-frequency, robotization.

For realizing the purpose of foregoing invention, pollutant aerobic biological degradation breath measuring method carries out according to the following steps:

(1) in air, dissolved oxygen sensor is checked;

(2) in tank, inject clear water, make solarization air cell, respiratory chamber import and export measuring chamber and respiratory chamber be arranged in clear water; The water temperature of setting in this tank is 5-50 ℃, reaches design temperature by the temperature in heating arrangements, refrigeration mechanism and the control gear control tank thereof;

(3) add the microorganism mixed liquor in solarization air cell, making its concentration is 200-5000mg/L;

(4) open aeration mechanism, bubbling air in solarization air cell, air mass flow is 5-1500ml/min, drives magnetic stirring apparatus respectively, and the stirring rotator of solarization air cell bottom and respiratory chamber bottom is rotated, speed of agitator is 20-200 commentaries on classics/min;

(5) open peristaltic pump, the flow of this peristaltic pump is 50-1000ml/min, makes the microorganism mixed liquor in the solarization air cell enter measurement chamber, the respiratory chamber of respiratory chamber import measuring chamber, measurement chamber, the solarization air cell of respiratory chamber outlet measuring chamber by pipeline circulation;

(6) open oxygen and utilize rate test software, parameter is set: the physical channel of input signal, sample frequency f is 1-20s ^-1Oxygen utilizes rate test time step Δ t to be 10-300s, the sampling number N=f Δ t of each passage, the current value of input signal is 0.004-0.02A, overtime is integer greater than Δ t, the filtering exponent number is 3-9, and flow Q is the actual flow of peristaltic pump, and volume V is the actual volume 200-2000ml of respiratory chamber;

(7) when the temperature of the microorganism mixed liquor in solarization air cell and the respiratory chamber and the clear water temperature in the tank all reach design temperature 5-50 ℃, click oxygen and utilize START button in the rate test software:

1. the current signal to two dissolved oxygen sensors carries out the digital filtering processing;

The current signal of two dissolved oxygen sensors after 2. will handling through digital filtering utilizes linear function to be converted to the dissolved oxygen concentration value C of respiratory chamber import and export microorganism ₁And C ₂

3. calculate i oxygen respectively and utilize the interior dissolved oxygen concentration value C of rate test time step Δ t ₁With C ₂Arithmetic mean With

And on user interface, show in real time; Meanwhile, oxygen is utilized dissolved oxygen concentration value C in the rate test time step Δ t ₁With C ₂Carry out linear fit, obtain C ₁And C ₂Time dependent speed ${\left(\frac{{\mathrm{dC}}_1}{\mathrm{dt}}\right)}_i$ With ${\left(\frac{{\mathrm{dC}}_2}{\mathrm{dt}}\right)}_i;$

4. utilize formula $R_i=\frac{Q}{V}[{\left(\stackrel{\‾}{C_1}\right)}_i-{\left(\stackrel{\‾}{C_2}\right)}_i]-{\left(\frac{{\mathrm{dC}}_2}{\mathrm{dt}}\right)}_i$ Calculate oxygen and utilize speed, and online in real time shows and instant the preservation;

In the following formula:

I-oxygen that measures utilizes the sequence number of speed;

R _i-the i oxygen utilizes speed, mgO ₂/ (Lmin);

Q-peristaltic pump flow, ml/min;

V-respiratory chamber volume, ml;

The arithmetic mean of-respiratory chamber import microorganism mixed liquor dissolved oxygen concentration in i oxygen utilizes rate test time step Δ t, mgO ₂/ L;

The arithmetic mean of-respiratory chamber outlet microorganism mixed liquor dissolved oxygen concentration in i oxygen utilizes rate test time step Δ t, mgO ₂/ L;

-respiratory chamber outlet microorganism mixed liquor dissolved oxygen concentration rate over time in i oxygen utilizes rate test time step Δ t, mgO ₂/ (Lmin);

(8) utilize rate stabilization when the oxygen in microorganism mixed liquor endogenous respiration stage utilizes speed when oxygen, add testing sample in the microorganism mixed liquor in solarization air cell, the oxygen that utilizes the rate test computed in software to add the microorganism mixed liquor behind the testing sample with oxygen utilizes speed, and online demonstration and instant the preservation.

2. when needs are checked dissolved oxygen sensor, carry out according to the following steps:

(1) the mutual drift check of dissolved oxygen sensor: dissolved oxygen sensor is placed air, on transmitter, dissolved oxygen sensor is checked, obtain import and export dissolved oxygen sensor reading C according to the conventional service regeulations of dissolved oxygen sensor ₁(0) and C ₂(0), calculates △ C (0)=C ₁(0)-C ₂(0);

(2) drift certainly of dissolved oxygen sensor check: the microorganism mixed liquor in solarization air cell, measuring chamber and the passage is cleaned out, in solarization air cell, inject clear water, obtain the arithmetic mean of i respiratory rate test duration of clear water step delta t internal respiration chamber import and export clear water dissolved oxygen concentration

With

And rate of change

With

(3) the soft check of dissolved oxygen sensor: on nucleus correcting program, call Δ C (0), $\frac{{\mathrm{dC}}_{1b}}{\mathrm{dt}},\frac{{\mathrm{dC}}_{2b}}{\mathrm{dt}},{\left(\stackrel{\‾}{C_1}\right)}_i,{\left(\stackrel{\‾}{C_2}\right)}_i$ With

Calculate ${{\left(\stackrel{\‾}{C_1}\right)}_i}^{*}={\left(\stackrel{\‾}{C_1}\right)}_i-\mathrm{\ΔC}\left(0\right)-\mathrm{\Δt}\·\underset{j=1}{\overset{i}{\mathrm{\Σ}}}{\left(\frac{{\mathrm{dC}}_{1b}}{\mathrm{dt}}\right)}_j$ With ${{\left(\stackrel{\‾}{C_2}\right)}_i}^{*}={\left(\stackrel{\‾}{C_2}\right)}_i-\mathrm{\Δt}\·\underset{j=1}{\overset{i}{\mathrm{\Σ}}}{\left(\frac{{\mathrm{dC}}_{2b}}{\mathrm{dt}}\right)}_j,$ Utilize after checking

With

Calculate ${R_i}^{*}=\frac{Q}{V}[{{\left(\stackrel{\‾}{C_1}\right)}_i}^{*}-{{\left(\stackrel{\‾}{C_2}\right)}_i}^{*}]-[{\left(\frac{{\mathrm{dC}}_2}{\mathrm{dt}}\right)}_i-{\left(\frac{{\mathrm{dC}}_{2b}}{\mathrm{dt}}\right)}_i],$ Oxygen after obtaining checking utilizes speed

And preserve.

The pollutant aerobic biological degradation breath measurement mechanism comprises housing, tank, respiratory chamber, solarization air cell, lay respectively at the magnetic agitation rotor in respiratory chamber and the solarization air cell bottom, correspondence is installed in the rotating mechanism of the magnetic agitation rotor in the housing of tank below and is installed in the control gear of the rotating mechanism of the magnetic agitation rotor on the housing side, peristaltic pump, aeration mechanism and pH sensor, the upper end of pH sensor is connected by the signal input part of signal transmssion line with a transmitter, be installed in the heating arrangements on the tank trailing flank, refrigeration mechanism and ebullator and be installed in heating arrangements on the surface of shell and the control gear of refrigeration mechanism, the upper end of two dissolved oxygen sensors connects with the signal input part of corresponding transmitter by signal transmssion line respectively, the signal output part of transmitter is connected with the signal input part of terminal box, and the signal output part of terminal box is connected with the computing machine that is inserted with data collecting card; The suction-chamber is advanced, the outlet measuring chamber is identical measuring chamber, each measuring chamber comprises that the upper cavity on top and the measurement cavity of bottom are communicated with along the axis sealing and fixing, two dissolved oxygen sensor lower ends are inserted respiratory chamber respectively and are advanced, in the measurement cavity of outlet measuring chamber bottom, fixedly connected with the measuring chamber upper cavity in the top of dissolved oxygen sensor, the upper end of the bottom of measuring chamber upper cavity and measurement cavity cooperates with the dissolved oxygen sensor activity and seals, described peristaltic pump is communicated with by the measurement cavity bottom of pipeline with solarization air cell and respiratory chamber import measuring chamber, the dissolved oxygen sensor lower end side the measurement cavity and the respiratory chamber of right respiratory chamber import measuring chamber be communicated with by pipeline.The measurement cavity bottom of the top of respiratory chamber and respiratory chamber outlet measuring chamber is communicated with by pipeline, the dissolved oxygen sensor lower end side the pipeline that communicates with solarization air cell is housed on the measurement cavity of right respiratory chamber outlet measuring chamber, solarization air cell, respiratory chamber and respiratory chamber advance, the outlet measuring chamber is positioned at the tank that fills clear water, the microorganism mixed liquor passes through peristaltic pump, pipeline is in solarization air cell, the measurement chamber of respiratory chamber import measuring chamber, respiratory chamber, the measurement chamber and the aeration indoor circulation of respiratory chamber outlet measuring chamber, solarization air cell is 3-5:1 with the ratio of the volume of respiratory chamber, and respiratory chamber advances, the measurement chamber of outlet measuring chamber is 1:100-300 with the ratio of the volume of respiratory chamber.

The present invention has following technique effect:

(1) be not subjected to the restriction of dissolved oxygen DO, the mass transfer coefficient that need not consider oxygen, entire system good constant-temperature effect, the suffered interference of dissolved oxygen sensor few, adopted filtering technique and linear fit algorithm, thereby the measuring accuracy height;

(2) obtain oxygen by the variation of using dissolved oxygen sensor to measure the liquid phase dissolved oxygen concentration and utilize speed, use high-frequency signal collecting device, thereby improved test frequency;

(3) developed oxygen voluntarily and utilized speed measurement software, the user interface of software close friend has improved automaticity, makes test process and test result directly perceived.

Description of drawings

Fig. 1 is the general structure synoptic diagram of measurement mechanism provided by the invention;

Fig. 2 is the theory structure synoptic diagram;

Fig. 3 is the vertical view of measuring chamber;

Fig. 4 is the cut-open view of the A-A among Fig. 3;

Fig. 5 installs vertical view behind the dissolved oxygen sensor for measuring chamber;

Fig. 6 is the cut-open view of the B-B among Fig. 5;

The process flow diagram that Fig. 7 gathers for the dissolved oxygen sensor current signal;

Fig. 8 utilizes the speed measurement software block diagram for oxygen;

Fig. 9 utilizes the user interface synoptic diagram of speed measurement software for oxygen;

Figure 10 is the process flow diagram of respiration measurement process;

Figure 11 is the structural representation that removes among Fig. 1 temperature control device behind left side and the upper surface housing;

Figure 12 is the temperature control device structural representation behind the back housing that removes among Fig. 1;

Figure 13 is the vertical view of temperature control device lower housing portion magnetic stirring apparatus;

Figure 14 is the C-C cut-open view among Figure 13;

At Fig. 1, Fig. 2, Fig. 4, Fig. 6, Figure 11, Figure 12, among Figure 14,1 is the microorganism mixed liquor, 2 is respiratory chamber, 3 is the magnetic agitation rotor of respiratory chamber bottom, 4 is respiratory chamber import measuring chamber, the 4-1 upper cavity, 4-2 is for measuring cavity, 4-3 is an O-ring seal, 4-4 is an O-ring seal, 5 are respiratory chamber outlet measuring chamber, 6 is pipeline, 7 is two dissolved oxygen sensors, 8 is the timing button of the magnetic agitation mechanism of respiratory chamber, 9 is the rotational speed regulation button of the magnetic agitation mechanism of respiratory chamber, 10 is the power switch of the magnetic agitation mechanism of respiratory chamber, 11 is peristaltic pump, 12 is the timing button of the magnetic agitation mechanism of solarization air cell, 13 is the rotational speed regulation button of the magnetic agitation mechanism of solarization air cell, 14 is the power switch of the magnetic agitation mechanism of solarization air cell, 15 is the housing of magnetic agitation mechanism, 16 is the magnetic agitation rotor of solarization air cell bottom, 17 is aeration head, 18 is solarization air cell, 19 is tank, 20 is aerator, 21 is the pH sensor, 22 is the temperature adjustment buttons of temperature control mechanism, 23 is the housing of temperature control mechanism, 24 is the heating work status indicator lamp of temperature control mechanism, 25 is the heating power supply switch of temperature control mechanism, 26 is the refrigeration work status indicator lamp of temperature control mechanism, 27 is the temperature control mechanism thermal light, 28 are temperature control mechanism refrigeration power switch, 29 is tank lack of water pilot lamp, 30 is signal transmssion line, and 31 is transmitter, and 32 is terminal box, 33 are inserted with the computing machine of data collecting card in being, 34 is bolt, and 35 is water circulating pump, and 36 is the control module of temperature control mechanism, 37 is condenser, 38 is refrigeration compressor, and 39 is air hose, and 40 is evaporator, 41 is the constant humidity well heater, 42 is the water hole on the tank trailing flank, and 43 is the water delivering orifice of water circulating pump, and 44 is temperature sensor, 45 is respiratory chamber bottom magnetic agitation rotor motor, 46 solarization air cell bottom magnetic agitation rotor motor, 47 is respiratory chamber bottom magnetic agitation magnet rotor, 48 is solarization air cell's bottom magnetic agitation magnet rotor.

Embodiment

When with concentration being the glucose solution of 20gCOD/L during as testing sample, this method is carried out according to the following steps:

(1) in air, dissolved oxygen sensor is checked;

(2) make the clear water in the tank remain on 20 ℃;

(3) add active sludge intermixture 4000ml in solarization air cell, concentration is 1000mgMLVSS/L;

(4) air mass flow that feeds in solarization air cell is 0.1L/min; Drive magnetic stirring apparatus respectively, the stirring rotator of solarization air cell bottom and respiratory chamber bottom is rotated, speed of agitator is 100 commentaries on classics/min;

(5) open peristaltic pump, making its flow is 200ml/min, makes active sludge intermixture by measurement chamber, respiratory chamber, the solarization air cell circulation of pipeline at respiratory chamber import and export measuring chamber;

(6) open oxygen and utilize rate test software, parameter is set: the physical channel is " 0 " and " 1 ", and sample frequency f is 10s ^-1, oxygen utilizes the rate test time step to be 20s, and the sampling number of each passage is 200, and the input current signal current value is 0.004-0.02A, and overtime is 30s, and the filtering exponent number is 7, and flow Q is 200ml/min,, volume V is 1000ml;

(7) when the temperature of the temperature of the active sludge intermixture in solarization air cell and the respiratory chamber and the clear water in the tank all reaches 20 ℃ of design temperatures, click oxygen utilizes the START button in the rate test software, software moves automatically, and current signal value, each oxygen that demonstration current " 0 " passage and " 1 " passage collect on user interface utilizes the dissolved oxygen concentration value C of rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean

With

And oxygen utilizes rate value;

(8) when oxygen utilizes rate stabilization in endogenous respiration speed, add glucose solution 4ml in the active sludge intermixture in solarization air cell, oxygen utilizes rate test software to calculate automatically, preserve and shows in real time and adds the dissolved oxygen concentration value C that each oxygen behind the glucose solution utilizes rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean

With

And oxygen utilizes rate value R _i

When with concentration being the sodium acetate solution of 20gCOD/L during as testing sample, this method is carried out according to the following steps:

(1) in air, dissolved oxygen sensor is checked;

(2) make the clear water in the tank remain on 25 ℃;

(3) add active sludge intermixture 4000ml in solarization air cell, concentration is 1500mgMLVSS/L;

(4) air mass flow that feeds in solarization air cell is 0.2L/min, drives magnetic stirring apparatus respectively, and the stirring rotator of solarization air cell bottom and respiratory chamber bottom is rotated, and speed of agitator is 100 commentaries on classics/min;

(5) open peristaltic pump, making its flow is 250ml/min, makes active sludge intermixture by measurement chamber, respiratory chamber, the solarization air cell circulation of pipeline at respiratory chamber import and export measuring chamber;

(6) open oxygen and utilize rate test software, parameter is set: the physical channel is " 0 " and " 1 ", and sample frequency f is 10s ^-1, oxygen utilizes the rate test time step to be 20s, and the sampling number of each passage is 200, and the input current signal current value is 0.004-0.02A, and overtime is 40s, and the filtering exponent number is 7, and flow Q is 250ml/min,, volume V is 1000ml;

(7) when the temperature of the temperature of the active sludge intermixture in solarization air cell and the respiratory chamber and the clear water in the tank all reaches 20 ℃ of design temperatures, click oxygen utilizes the START button in the rate test software, software moves automatically, and current signal value, each oxygen that demonstration current " 0 " passage and " 1 " passage collect on user interface utilizes the dissolved oxygen concentration value C of rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean

With

And oxygen utilizes rate value;

(8) when oxygen utilizes rate stabilization in endogenous respiration speed, add sodium acetate solution 6ml in the active sludge intermixture in solarization air cell, oxygen utilize rate test software to calculate automatically, preserve and show in real time add that each oxygen after the sodium acetate solution utilizes rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture separate oxygen concentration value C ₁And C ₂Arithmetic mean

With

And oxygen utilizes rate value R _i

When with municipal effluent during as testing sample, this method is carried out according to the following steps:

(1) in air, dissolved oxygen sensor is checked;

(2) make the clear water in the tank remain on 25 ℃;

(3) add active sludge intermixture 2000ml in solarization air cell, concentration is 3000mgMLVSS/L;

(4) air mass flow that feeds in solarization air cell is 0.2L/min, drives magnetic stirring apparatus respectively, and the stirring rotator of solarization air cell bottom and respiratory chamber bottom is rotated, and speed of agitator is 100 commentaries on classics/min;

(5) open peristaltic pump, making its flow is 250ml/min, makes active sludge intermixture by measurement chamber, respiratory chamber, the solarization air cell circulation of pipeline at respiratory chamber import and export measuring chamber;

(6) open oxygen and utilize rate test software, parameter is set: the physical channel is " 0 " and " 1 ", and sample frequency f is 10s ^-1, oxygen utilizes the rate test time step to be 30s, and the sampling number of each passage is 300, and the input current signal current value is 0.004-0.02A, and overtime is 40s, and the filtering exponent number is 7, and flow Q is 250ml/min,, volume V is 1000ml;

(7) when the temperature of the temperature of the active sludge intermixture in solarization air cell and the respiratory chamber and the clear water in the tank all reaches 25 ℃ of design temperatures, click oxygen utilizes the START button in the rate test software, software moves automatically, and current signal value, each oxygen that demonstration current " 0 " passage and " 1 " passage collect on user interface utilizes the dissolved oxygen concentration value C of rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean

With

And oxygen utilizes rate value;

(8) also aeration is saturated to dissolved oxygen DO fast the municipal effluent water sample to be heated to 25 ℃; When oxygen utilizes rate stabilization in endogenous respiration speed, add municipal effluent 2000ml in the active sludge intermixture in solarization air cell, oxygen utilizes rate test software to calculate automatically, preserve and shows in real time and adds the dissolved oxygen concentration value C that each oxygen behind the municipal effluent utilizes rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean

With And oxygen utilizes rate value R _i

The microorganism mixed liquor pumps into respiratory chamber import measuring chamber and respiratory chamber outlet measuring chamber by the peristaltic pump malleation, and the microorganism mixed liquor flows to the film surface perpendicular to the built-in dissolved oxygen sensor of measuring chamber.

Respiratory chamber import and export measuring chamber is last big, following two sections little right cylinders, has circular hole to communicate in these two sections right cylinders, and there is a round tube hole that cooperates with the dissolved oxygen sensor activity at the middle part, bottom of upper cavity, and screw thread is arranged in the upper end circular hole of upper cavity; Measure middle part, bottom, chamber and side round tube hole is arranged respectively, connecting tube is housed respectively on this round tube hole, the upper end circular hole internal fixation of measuring in the cavity has the rubber seal that cooperates with dissolved oxygen sensor, and the lower end of upper cavity is connected by thread seal with the upper end of measuring cavity.

The ratio of the volume of solarization air cell and respiratory chamber is 4:1, and the measurement chamber of respiratory chamber import and export measuring chamber is 1:200 with the ratio of the volume of respiratory chamber.

Claims (9)

1, a kind of pollutant aerobic biological degradation breath measuring method is characterized in that this method carries out according to the following steps:

(1) in air, dissolved oxygen sensor is checked;

(2) in tank, inject clear water, make solarization air cell, respiratory chamber import and export measuring chamber and respiratory chamber be arranged in clear water; The water temperature of setting in this tank is 5-50 ℃, reaches design temperature by the temperature in heating arrangements, refrigeration mechanism and the control gear control tank thereof;

(3) add the microorganism mixed liquor in solarization air cell, making its concentration is 200-5000mg/L;

(4) open aeration mechanism, bubbling air in solarization air cell, air mass flow is 5-1500ml/min, drives magnetic stirring apparatus respectively, and the stirring rotator of solarization air cell bottom and respiratory chamber bottom is rotated, speed of agitator is 20-200 commentaries on classics/min;

(5) open peristaltic pump, the flow of this peristaltic pump is 50-1000ml/min, makes the microorganism mixed liquor in the solarization air cell enter measurement chamber, the respiratory chamber of respiratory chamber import measuring chamber, measurement chamber, the solarization air cell of respiratory chamber outlet measuring chamber by pipeline circulation;

(6) open oxygen and utilize rate test software, parameter is set: the physical channel of input signal, sample frequency f is 1-20s ^-1Oxygen utilizes rate test time step Δ t to be 10-300s, the sampling number N=f Δ t of each passage, the current value of input signal is 0.004-0.02A, overtime is integer greater than Δ t, the filtering exponent number is 3-9, and flow Q is the actual flow of peristaltic pump, and volume V is the actual volume 200-2000ml of respiratory chamber;

(7) when the temperature of the microorganism mixed liquor in solarization air cell and the respiratory chamber and the clear water temperature in the tank all reach design temperature 5-50 ℃, click oxygen and utilize START button in the rate test software:

1. the current signal to two dissolved oxygen sensors carries out the digital filtering processing;

The current signal of two dissolved oxygen sensors after 2. will handling through digital filtering utilizes linear function to be converted to the dissolved oxygen concentration value C of respiratory chamber import and export microorganism ₁And C ₂

3. calculate i oxygen respectively and utilize the interior dissolved oxygen concentration value C of rate test time step Δ t ₁With C ₂Arithmetic mean

With

And on user interface, show in real time; Meanwhile, oxygen is utilized dissolved oxygen concentration value C in the rate test time step Δ t ₁With C ₂Carry out linear fit, obtain C ₁And C ₂Time dependent speed ${\left(\frac{{\mathrm{dC}}_1}{\mathrm{dt}}\right)}_i,$ With ${\left(\frac{{\mathrm{dC}}_2}{\mathrm{dt}}\right)}_i;$

4. utilize formula $R_i=\frac{Q}{V}[{\left(\stackrel{\‾}{C_1}\right)}_i-{\left(\stackrel{\‾}{C_2}\right)}_i]-{\left(\frac{{\mathrm{dC}}_2}{\mathrm{dt}}\right)}_i$ Calculate oxygen and utilize speed, and online in real time shows and instant the preservation;

In the following formula:

I-oxygen that measures utilizes the sequence number of speed;

R _i-the i oxygen utilizes speed, mgO ₂/ (Lmin);

Q-peristaltic pump flow, ml/min;

V-respiratory chamber volume, ml;

The arithmetic mean of-respiratory chamber import microorganism mixed liquor dissolved oxygen concentration in i oxygen utilizes rate test time step Δ t, mgO ₂/ L;

The arithmetic mean of-respiratory chamber outlet microorganism mixed liquor dissolved oxygen concentration in i oxygen utilizes rate test time step Δ t, mgO ₂/ L;

-respiratory chamber outlet microorganism mixed liquor dissolved oxygen concentration rate over time in i oxygen utilizes rate test time step Δ t, mgO ₂/ (Lmin);

(8) utilize rate stabilization when the oxygen in microorganism mixed liquor endogenous respiration stage utilizes speed when oxygen, add testing sample in the microorganism mixed liquor in solarization air cell, the oxygen that utilizes the rate test computed in software to add the microorganism mixed liquor behind the testing sample with oxygen utilizes speed, and online demonstration and instant the preservation.

2. pollutant aerobic biological degradation breath measuring method according to claim 1 is characterized in that carrying out according to the following steps when needs are checked dissolved oxygen sensor:

(1) the mutual drift check of dissolved oxygen sensor: dissolved oxygen sensor is placed air, on transmitter, dissolved oxygen sensor is checked, obtain import and export dissolved oxygen sensor reading C according to the conventional service regeulations of dissolved oxygen sensor ₁(0) and C ₂(0), calculates Δ C (0)=C ₁(0)-C ₂(0);

(2) drift certainly of dissolved oxygen sensor check: the microorganism mixed liquor in solarization air cell, measuring chamber and the passage is cleaned out, in solarization air cell, inject clear water, obtain the arithmetic mean of i respiratory rate test duration of clear water step delta t internal respiration chamber import and export clear water dissolved oxygen concentration

With

And rate of change

With

(3) the soft check of dissolved oxygen sensor: on nucleus correcting program, call Δ C (0),

With

Calculate ${{\left(\stackrel{\‾}{C_1}\right)}_i}^{*}={\left(\stackrel{\‾}{C_1}\right)}_i-\mathrm{\ΔC}\left(0\right)-\mathrm{\Δt}\·\underset{j=1}{\overset{i}{\mathrm{\Σ}}}{\left(\frac{{\mathrm{dC}}_{1b}}{\mathrm{dt}}\right)}_j$ ${{\left(\stackrel{\‾}{C_2}\right)}_i}^{*}={\left(\stackrel{\‾}{C_2}\right)}_i-\mathrm{\Δt}\·\underset{j=1}{\overset{i}{\mathrm{\Σ}}}{\left(\frac{{\mathrm{dC}}_{2b}}{\mathrm{dt}}\right)}_j,$ Utilize after checking

With Calculate ${R_i}^{*}=\frac{Q}{V}[{{\left(\stackrel{\‾}{C_1}\right)}_i}^{*}-{{\left(\stackrel{\‾}{C_2}\right)}_i}^{*}]-[{\left(\frac{{\mathrm{dC}}_2}{\mathrm{dt}}\right)}_i-{\left(\frac{{\mathrm{dC}}_{2b}}{\mathrm{dt}}\right)}_i],$ Oxygen after obtaining checking utilizes speed

And preserve.

3. pollutant aerobic biological degradation breath measuring method according to claim 1 is characterized in that this method is carried out according to the following steps when with concentration being the glucose solution of 20gCOD/L during as testing sample:

(1) in air, dissolved oxygen sensor is checked;

(2) make the clear water in the tank remain on 20 ℃;

(3) add active sludge intermixture 4000ml in solarization air cell, concentration is 1000mgMLVSS/L;

(4) air mass flow that feeds in solarization air cell is 0.1L/min; Drive magnetic stirring apparatus respectively, the stirring rotator of solarization air cell bottom and respiratory chamber bottom is rotated, speed of agitator is 100 commentaries on classics/min;

(5) open peristaltic pump, making its flow is 200ml/min, makes active sludge intermixture by measurement chamber, respiratory chamber, the solarization air cell circulation of pipeline at respiratory chamber import and export measuring chamber;

(6) open oxygen and utilize rate test software, parameter is set: the physical channel is " 0 " and " 1 ", and sample frequency f is 10s ^-1, oxygen utilizes the rate test time step to be 20s, and the sampling number of each passage is 200, and the input current signal current value is 0.004-0.02A, and overtime is 30s, and the filtering exponent number is 7, and flow Q is 200ml/min,, volume V is 1000ml;

(7) when the temperature of the temperature of the active sludge intermixture in solarization air cell and the respiratory chamber and the clear water in the tank all reaches 20 ℃ of design temperatures, click oxygen utilizes the START button in the rate test software, software moves automatically, and current signal value, each oxygen that demonstration current " 0 " passage and " 1 " passage collect on user interface utilizes the dissolved oxygen concentration value C of rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean

With And oxygen utilizes rate value;

(8) when oxygen utilizes rate stabilization in endogenous respiration speed, add glucose solution 4ml in the active sludge intermixture in solarization air cell, oxygen utilizes rate test software to calculate automatically, preserve and shows in real time and adds the dissolved oxygen concentration value C that each oxygen behind the glucose solution utilizes rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean

With

And oxygen utilizes rate value R _i

4. pollutant aerobic biological degradation breath measuring method according to claim 1 is characterized in that this method is carried out according to the following steps when with concentration being the sodium acetate solution of 20gCOD/L during as testing sample:

(1) in air, dissolved oxygen sensor is checked;

(2) make the clear water in the tank remain on 25 ℃;

(3) add active sludge intermixture 4000ml in solarization air cell, concentration is 1500mgMLVSS/L;

(4) air mass flow that feeds in solarization air cell is 0.2L/min, drives magnetic stirring apparatus respectively, and the stirring rotator of solarization air cell bottom and respiratory chamber bottom is rotated, and speed of agitator is 100 commentaries on classics/min;

(5) open peristaltic pump, making its flow is 250ml/min, makes active sludge intermixture by measurement chamber, respiratory chamber, the solarization air cell circulation of pipeline at respiratory chamber import and export measuring chamber;

(6) open oxygen and utilize rate test software, parameter is set: the physical channel is " 0 " and " 1 ", and sample frequency f is 10s ^-1, oxygen utilizes the rate test time step to be 20s, and the sampling number of each passage is 200, and the input current signal current value is 0.004-0.02A, and overtime is 40s, and the filtering exponent number is 7, and flow Q is 250ml/min,, volume V is 1000ml;

(7) when the temperature of the temperature of the active sludge intermixture in solarization air cell and the respiratory chamber and the clear water in the tank all reaches 20 ℃ of design temperatures, click oxygen utilizes the START button in the rate test software, software moves automatically, and current signal value, each oxygen that demonstration current " 0 " passage and " 1 " passage collect on user interface utilizes the dissolved oxygen concentration value C of rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean With

And oxygen utilizes rate value;

(8) when oxygen utilizes rate stabilization in endogenous respiration speed, add sodium acetate solution 6ml in the active sludge intermixture in solarization air cell, oxygen utilizes rate test software to calculate automatically, preserve and shows in real time and adds the dissolved oxygen concentration value C that each oxygen after the sodium acetate solution utilizes rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean With

And oxygen utilizes rate value R _i

5. pollutant aerobic biological degradation breath measuring method according to claim 1 is characterized in that when with municipal effluent during as testing sample, this method is carried out according to the following steps:

(1) in air, dissolved oxygen sensor is checked;

(2) make the clear water in the tank remain on 25 ℃;

(3) add active sludge intermixture 2000ml in solarization air cell, concentration is 3000mgMLVSS/L;

(4) air mass flow that feeds in solarization air cell is 0.2L/min, drives magnetic stirring apparatus respectively, and the stirring rotator of solarization air cell bottom and respiratory chamber bottom is rotated, and speed of agitator is 100 commentaries on classics/min;

(5) open peristaltic pump, making its flow is 250ml/min, makes active sludge intermixture by measurement chamber, respiratory chamber, the solarization air cell circulation of pipeline at respiratory chamber import and export measuring chamber;

(6) open oxygen and utilize rate test software, parameter is set: the physical channel is " 0 " and " 1 ", and sample frequency f is 10s ^-1, oxygen utilizes the rate test time step to be 30s, and the sampling number of each passage is 300, and the input current signal current value is 0.004-0.02A, and overtime is 40s, and the filtering exponent number is 7, and flow Q is 250ml/min,, volume V is 1000ml;

(7) when the temperature of the temperature of the active sludge intermixture in solarization air cell and the respiratory chamber and the clear water in the tank all reaches 25 ℃ of design temperatures, click oxygen utilizes the START button in the rate test software, software moves automatically, and current signal value, each oxygen that demonstration current " 0 " passage and " 1 " passage collect on user interface utilizes the dissolved oxygen concentration value C of rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean

With

And oxygen utilizes rate value;

(8) also aeration is saturated to dissolved oxygen DO fast the municipal effluent water sample to be heated to 25 ℃; When oxygen utilizes rate stabilization in endogenous respiration speed, add municipal effluent 2000ml in the active sludge intermixture in solarization air cell, oxygen utilizes rate test software to calculate automatically, preserve and shows in real time and adds the dissolved oxygen concentration value C that each oxygen behind the municipal effluent utilizes rate test time step Δ t internal respiration chamber import and outlet active sludge intermixture ₁And C ₂Arithmetic mean With

And oxygen utilizes rate value R _i

6. pollutant aerobic biological degradation breath measuring method according to claim 1, it is characterized in that the microorganism mixed liquor pumps into respiratory chamber import measuring chamber and respiratory chamber outlet measuring chamber by the peristaltic pump malleation, the microorganism mixed liquor flows to the film surface perpendicular to the built-in dissolved oxygen sensor of measuring chamber.

7. device of implementing the described pollutant aerobic biological degradation breath measuring method of claim 1, comprise housing, tank, respiratory chamber, solarization air cell, lay respectively at the magnetic agitation rotor in respiratory chamber and the solarization air cell bottom, correspondence is installed in the rotating mechanism of the magnetic agitation rotor in the housing of tank below and is installed in the control gear of the rotating mechanism of the magnetic agitation rotor on the housing side, peristaltic pump, aeration mechanism and pH sensor, the upper end of pH sensor is connected by the signal input part of signal transmssion line with a transmitter, be installed in the heating arrangements on the tank trailing flank, refrigeration mechanism and ebullator and be installed in heating arrangements on the surface of shell and the control gear of refrigeration mechanism, the upper end of two dissolved oxygen sensors connects with the signal input part of corresponding transmitter by signal transmssion line respectively, the signal output part of transmitter is connected with the signal input part of terminal box, and the signal output part of terminal box is connected with the computing machine that is inserted with data collecting card; It is characterized in that respiratory chamber advances, the outlet measuring chamber is identical measuring chamber, each measuring chamber comprises that the upper cavity on top and the measurement cavity of bottom are communicated with along the axis sealing and fixing, two dissolved oxygen sensor lower ends are inserted respiratory chamber respectively and are advanced, in the measurement cavity of outlet measuring chamber bottom, fixedly connected with the measuring chamber upper cavity in the top of dissolved oxygen sensor, the upper end of the bottom of measuring chamber upper cavity and measurement cavity cooperates with the dissolved oxygen sensor activity and seals, described peristaltic pump is communicated with by the measurement cavity bottom of pipeline with solarization air cell and respiratory chamber import measuring chamber, the dissolved oxygen sensor lower end side the measurement cavity and the respiratory chamber of right respiratory chamber import measuring chamber be communicated with by pipeline, the measurement cavity bottom of the top of respiratory chamber and respiratory chamber outlet measuring chamber is communicated with by pipeline, the dissolved oxygen sensor lower end side the pipeline that communicates with solarization air cell is housed on the measurement cavity of right respiratory chamber outlet measuring chamber, solarization air cell, respiratory chamber and respiratory chamber advance, the outlet measuring chamber is positioned at the tank that fills clear water, the microorganism mixed liquor passes through peristaltic pump, pipeline is in solarization air cell, the measurement chamber of respiratory chamber import measuring chamber, respiratory chamber, the measurement chamber and the aeration indoor circulation of respiratory chamber outlet measuring chamber, solarization air cell is 3-5:1 with the ratio of the volume of respiratory chamber, and respiratory chamber advances, the measurement chamber of outlet measuring chamber is 1:100-300 with the ratio of the volume of respiratory chamber.

8. pollutant aerobic biological degradation breath measurement mechanism according to claim 7, it is characterized in that respiratory chamber import and export measuring chamber is last big, following two sections little right cylinders, there is circular hole to communicate in these two sections right cylinders, there is a round tube hole that cooperates with the dissolved oxygen sensor activity at the middle part, bottom of upper cavity, in the upper end circular hole of upper cavity screw thread is arranged; Measure middle part, bottom, chamber and side round tube hole is arranged respectively, connecting tube is housed respectively on this round tube hole, the upper end circular hole internal fixation of measuring in the cavity has the rubber seal that cooperates with dissolved oxygen sensor, and the lower end of upper cavity is connected by thread seal with the upper end of measuring cavity.

9. pollutant oxygen consumption biodegradation respiration measurement device according to claim 7, the ratio that it is characterized in that the volume of solarization air cell and respiratory chamber is 4:1, the measurement chamber of respiratory chamber import and export measuring chamber is 1:200 with the ratio of the volume of respiratory chamber.

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