CN100489110C - Method for producing enzyme modified soybean lecithin - Google Patents
Method for producing enzyme modified soybean lecithin Download PDFInfo
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- CN100489110C CN100489110C CNB2003101130339A CN200310113033A CN100489110C CN 100489110 C CN100489110 C CN 100489110C CN B2003101130339 A CNB2003101130339 A CN B2003101130339A CN 200310113033 A CN200310113033 A CN 200310113033A CN 100489110 C CN100489110 C CN 100489110C
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Abstract
The present invention provides a process for preparing enzyme modified soya bean lecithin, wherein phospholipase A2 is added therein according to 2000IU/100g-4000IU/100g concentrating phosphatide, CaCl2 is added therein according to 0.003-0.005 mol/L, the temperature is kept constant at 40-60 DEG C, reacting for 6-8 h; composite prolease is added therein according to 10000IU/g-20000IU/g, with pH value being controlled at 6-8, reacting for 3-5 h; the celvacene greases is removed by centrifugating for 10-20 minutes. By modifying soya bean lecithin through phospholipase A2, the process in accordance with the present invention not only improves process specific characters such as the HLB values and the lecithoid water-solubility, but also is free of introduction of chemical groups, being characterized by safety, reliability, limitation independence, broad applications and remarkable effect.
Description
[technical field]
The present invention relates to a kind of production method of soybean phospholipid, specifically, relate to a kind of production method of enzyme-modified soybean phospholipid.
[background technology]
Phosphatide is the main fat again that occurring in nature exists, and all contains phosphatide in nearly all biology, mainly is present in the seed and microorganism of brain, the heart, liver and blood, breast, plant of animal.What content was the highest is yolk, soybean.Phospholipid prod main on the market all derives from soybean.Take off in the pin technology at grease, separate, create by explained hereafter such as concentrating, refining again with the aquation grease.
Phosphatide had both contained lipophilic lipid acid one class, contained hydrophilic phosphate radical again, was a kind of natural amphoterics, therefore was widely used in every field such as food, feed, light industry, chemical industry, medicine.But because the flowability of phosphatide is poor, water-soluble bad, promptly its hydrophile-lipophile balance value (HLB value) is low and single, and concentrated phosphatide is 4, and powdered phospholipid is 6, serious restriction its application.
In order to make full use of good characteristics such as its trophicity, emulsifying property, diffusion wettability, constantly widen its Application Areas, improve result of use, carried out a large amount of research both at home and abroad, by chemistry, physics and enzyme process phosphatide is carried out modification, strengthen its flowability, water-soluble and increase operation rate.
ZL00110396.2 discloses a kind of making method of modified soy bean lipoid, in concentrated phosphatide pump such as reactor after the dehydration, add aceticanhydride and hydrogen peroxide, carry out acetylize and hydroxylating, and add in good time light alkali carry out in and modification, again the phosphatide after the modification of acyl hydroxyl association reaction is entered falling-film evaporator, carry out TRANSIENT HIGH TEMPERATURE rapid evaporation deodorization and dehydration; Then dehydration by evaporation is separated removal of contamination with the product that removes peculiar smell by whizzer.But traditional chemical modification because of having introduced other chemical substance, in use has been subjected to certain restriction.
[summary of the invention]
The object of the present invention is to provide a kind of phospholipase A that passes through
2Soybean phospholipid is carried out modification, improve its HLB value, strengthen the water-soluble production method that waits the soybean phospholipid of processing characteristics of phosphatide, this method does not have the introducing of chemical group, and is safe and reliable, unrestricted, is widely used, and effect is remarkable.
The object of the present invention is achieved like this: concentrated phosphatide is added reactor, be preheated to 40～60 ℃, regulating the pH value is 6～8; Press 2000IU/100g～4000IU/100g concentrated phosphatide and add phospholipase A
2, add CaCl by 0.003～0.005mol/L
2, stir; Constant temperature reacted 6～8 hours at 40 ℃～60 ℃; Add compound protease, consumption 10000IU/g～20000IU/g, control pH value 6～8 was reacted 3～5 hours; Reactant is added whizzer, under rotating speed 4000～6000rpm condition, centrifugal 10～20 minutes, remove degrease.
Preferably, concentrated phosphatide is added reactor, be preheated to 45～55 ℃, regulate pH value 6～8; Press 2500IU/100g～3500IU/100g concentrated phosphatide and add phospholipase A
2, add CaCl by 0.0033～0.0045mol/L
2, stir; Constant temperature reacted 6～8 hours at 45 ℃～55 ℃; Add compound protease, consumption 15000IU/g～18000IU/g, control pH value 6～8 was reacted 3～5 hours; Reactant is added whizzer, under rotating speed 4500～5500rpm condition, centrifugal 10～20 minutes, remove degrease.
Best, concentrated phosphatide is added reactor, be preheated to 50 ℃, regulate pH value 7; Press the 3000IU/100g concentrated phosphatide and add phospholipase A
2, press 0.004mol/L and add CaCl
2, stir; Constant temperature reacted 7 hours at 50 ℃; Add compound protease, consumption 15000IU/g, control pH value 7 was reacted 3 hours; Reactant is added whizzer, under rotating speed 5000rpm condition, centrifugal 15 minutes, remove degrease.
The present invention has following advantage:
1. the reaction conditions gentleness need not high temperature, high pressure.
2. product functionality is strong: the HLB value improves, and water-soluble, emulsifying property, diffusion wettability are improved.
3. safety: no chemical group is introduced, and product safety is reliable, uses unrestricted.
4. have wide range of applications: because of its functional raising, result of use is good, can be widely used in a plurality of fields such as light industry, chemical industry, food, medicine.
[embodiment]
Embodiment 1
Take by weighing the 50kg concentrated phosphatide and add in the reactor, attemperation reaches 60 ℃, and the pH value is 7, adds the phospholipase A of 1,250,000 IU
2And 17g CaCl
2Stir, under 60 ℃ of constant temperatures, reacted 8 hours.The compound protease that adds 1,000,000 IU, control pH value is 8, reacts 4 hours.Reactant adds whizzer, and under the 5000rpm rotating speed, centrifugal 10min removes degrease, and residuum is the modified phospholipid product.
Embodiment 2
Take by weighing the 50kg concentrated phosphatide and add in the reactor, attemperation reaches 40 ℃, and the pH value is 6, adds the phospholipase A of 1,000,000 IU
2And 17g CaCl
2Stir, under 45 ℃ of constant temperatures, reacted 8 hours.The compound protease that adds 800,000 IU, control pH value is 8, reacts 3 hours.Reactant adds whizzer, and under the 4000rpm rotating speed, centrifugal 15min removes degrease, and residuum is the modified phospholipid product.
Embodiment 3
Take by weighing the 50kg concentrated phosphatide and add in the reactor, attemperation reaches 50 ℃, and the pH value is 8, adds the phospholipase A of 2,000,000 IU
2And 17g CaCl
2Stir, under 55 ℃ of constant temperatures, reacted 8 hours.The compound protease that adds 1,200,000 IU, control pH value is 8, reacts 5 hours.Reactant adds whizzer, and under the 6000rpm rotating speed, centrifugal 20min removes degrease, and residuum is the modified phospholipid product.
Claims (3)
1, a kind of production method of enzyme-modified soybean phospholipid adds reactor with concentrated phosphatide, is preheated to 40～60 ℃, and regulating the pH value is 6～8, it is characterized in that: press 2000IU/100g～4000IU/100g concentrated phosphatide and add phospholipase A
2, add CaCl by 0.003～0.005mol/L
2, stir; Constant temperature reacted 6～8 hours at 40 ℃～60 ℃; Add compound protease, consumption 10000IU/g～20000IU/g, control pH value 6～8 was reacted 3～5 hours; Reactant is added whizzer, under rotating speed 4000～6000rpm condition, centrifugal 10～20 minutes, remove degrease.
2, the production method of a kind of enzyme-modified soybean phospholipid according to claim 1 is characterized in that: concentrated phosphatide is added reactor, be preheated to 45～55 ℃, regulating the pH value is 6～8; Press 2500IU/100g～3500IU/100g concentrated phosphatide and add phospholipase A
2, add CaCl by 0.0033～0.0045mol/L
2, stir; Constant temperature reacted 6～8 hours at 45 ℃～55 ℃; Add compound protease, consumption 15000IU/g～18000IU/g, control pH value is 6～8, reacts 3～5 hours; Reactant is added whizzer, under rotating speed 4500～5500rpm condition, centrifugal 10～20 minutes, remove degrease.
3, the production method of a kind of enzyme-modified soybean phospholipid according to claim 1 and 2 is characterized in that: concentrated phosphatide is added reactor, be preheated to 50 ℃, regulating the pH value is 7; Press the 3000IU/100g concentrated phosphatide and add phospholipase A
2, press 0.004mol/L and add CaCl
2, stir; Constant temperature reacted 7 hours at 50 ℃; Add compound protease, consumption 15000IU/g, control pH value is 7, reacts 3 hours; Reactant is added whizzer, under rotating speed 5000rpm condition, centrifugal 15 minutes, remove degrease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101130339A CN100489110C (en) | 2003-12-25 | 2003-12-25 | Method for producing enzyme modified soybean lecithin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101130339A CN100489110C (en) | 2003-12-25 | 2003-12-25 | Method for producing enzyme modified soybean lecithin |
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| CN1632127A CN1632127A (en) | 2005-06-29 |
| CN100489110C true CN100489110C (en) | 2009-05-20 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766533B (en) * | 2012-08-21 | 2013-09-11 | 东北农业大学 | Biological enzyme demulsification method for extracting soybean oil and fat by aqueous enzymatic method |
| CN104054895A (en) * | 2014-06-20 | 2014-09-24 | 天津鹤喜园磷脂科技有限公司 | Method for producing water-soluble modified phospholipid and formula thereof |
| CN105087696B (en) * | 2015-10-13 | 2019-01-01 | 李桂华 | A kind of enzyme modified soybean lecithin industrialization quantity-produced preparation method |
| CN107996915B (en) * | 2017-12-20 | 2021-04-27 | 山东甄沃食品股份有限公司 | Blueberry pulp and processing method thereof |
| CN112125747A (en) * | 2020-06-04 | 2020-12-25 | 单舒馨 | Microbial agent for enzymolysis of soybean phospholipid |
| CN111675578A (en) * | 2020-06-09 | 2020-09-18 | 临沂明英工贸有限公司 | Fruit swelling coloring microbial agent |
| CN111733193B (en) * | 2020-07-22 | 2021-12-07 | 河北德嵩生物科技有限公司 | Production method of phospholipid with good water solubility and high temperature resistance |
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2003
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Non-Patent Citations (4)
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| 大豆磷脂的功能特性及应用. 张强，王喜泉，朱秀清，周玉伦.大豆通报，第3期. 1994 |
| 大豆磷脂的功能特性及应用. 张强，王喜泉，朱秀清，周玉伦.大豆通报，第3期. 1994 * |
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| 磷脂的酶法改性技术进展. 谷利伟，曹宪荣，赵金兰.中国油脂，第24卷第6期. 1999 * |
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Assignee: Hagaoke Soybean Food Co., Ltd. Assignor: Soybean Tech. Development and Research Center, Heilongjiang Prov. Contract record no.: 2011230000240 Denomination of invention: Method for producing enzyme modified soybean lecithin Granted publication date: 20090520 License type: Exclusive License Open date: 20050629 Record date: 20110829 |
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Granted publication date: 20090520 Termination date: 20151225 |
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