CN100484965C - Preparation of carboxymethyl chitosan and its application in tissue surface engineering - Google Patents
Preparation of carboxymethyl chitosan and its application in tissue surface engineering Download PDFInfo
- Publication number
- CN100484965C CN100484965C CNB2005100949239A CN200510094923A CN100484965C CN 100484965 C CN100484965 C CN 100484965C CN B2005100949239 A CNB2005100949239 A CN B2005100949239A CN 200510094923 A CN200510094923 A CN 200510094923A CN 100484965 C CN100484965 C CN 100484965C
- Authority
- CN
- China
- Prior art keywords
- chitosan
- application
- preparation
- carboxymethyl chitosan
- tissue surface
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The invention discloses a method for preparing carboxymethyl chitosan and its application in the tissue surface engineering, preparing PLA, PLGA microballoon with clean surface by using the surface active and biocompatibility possessed by carboxymethyl chitosan which is used as a new surface activator; when used as tissue surface modifier, it can modify the blood vessel cell shape and tissues among cells.
Description
Technical field
The present invention relates to a kind of structure of cm-chitosan and at preparation PLA, PLGA microballoon and in the pattern of regulating vascular cell and the application of tissue.
Background technology
Chitin extensively is present in the shell of shrimp crab) partially deacetylated derivative.It is the linear glycan that N-acetyl Portugal polyamine random arrangement that a kind of D-Portugal polyamine that is linked to each other by β (1 → 4) and content do not wait is got up, it is the unique alkaline polysaccharide of occurring in nature, its intramolecularly had not only contained hydrophilic group and hydrophobic group simultaneously but also contain the amino and the hydroxyl of coordination ability and derivative reaction, and these character have determined it to have widespread use in different field.
Chitin and derivative thereof have air permeability and good, film-forming properties, water absorbability, degradation property and active reactivity, make chitin and derivative thereof demonstrate its distinctive using value at numerous areas such as weaving, printing and dyeing, papermaking, medicine, food, chemical industry, environmental protection.Have extremely strong hydrogen bond action in the chitin molecule, make its water insoluble and common organic solvents, the chitosan that takes off behind the acetyl is only soluble in diluted acid, and this has just greatly limited fundamental research and widespread use exploitation thereof of chitin and chitosan.
Summary of the invention
The objective of the invention is to provide a kind of and prepare the novel surfactant cm-chitosan of polyester microsphere and in the tissue surface application in engineering.
Technical scheme of the present invention is:
One, the novel surfactant cm-chitosan of preparation polyester microsphere
With the chitosan powder, use the NaOH solution soaking, solution is squeezed into filter cake shape with chitosan after filtering, and changes in three mouthfuls of containers.Be dissolved in Virahol with a certain amount of Mono Chloro Acetic Acid, the back adds in the filter cake, stoichiometric number hour under the room temperature, and suction filtration, extruding remove and desolvate.In the gained solid, add entry, stir and make its dissolving, transfer the pH value of solution value to neutral, add dehydrated alcohol in the supernatant liquor behind the solution centrifugal, have the flocculence precipitation to separate out with HCl.With solution centrifugal, flocks is extracted after leaving standstill, behind absolute ethanol washing, placed the baking oven oven dry can obtain cm-chitosan.The structural formula of this cm-chitosan is:
The sign of cm-chitosan
Characterize experiment 1: the relation between the surface tension of cm-chitosan and water
Fig. 1 shows that the surface tension of cm-chitosan is right.As shown in Figure 1, cm-chitosan can reduce the surface tension of water effectively.
Characterize experiment 2: the cell compatibility experiment
Add the cm-chitosan of the different concns of 1/5 volume in cell culture fluid, the growth of cell is influenced hardly, illustrates that cm-chitosan has better biocompatibility.
Two, cm-chitosan is in the tissue surface application in engineering
Cm-chitosan prepares the polyester microsphere of surface cleaning and cm-chitosan is regulated cell as biomaterial surface properties-correcting agent pattern and intercellular tissue as green surfactant.
Illustrate that below by example cm-chitosan is in the tissue surface application in engineering
Application example
Application example 1: cm-chitosan is used as PLA and the PLGA microballoon that emulsifying agent prepares surface cleaning
Present solvent evaporated method prepares Biodegradable polyester, and (as PLA, PLGA) particulate is used for medicine sustained release carrier, and the most frequently used emulsifying agent is polyvinyl alcohol (PVA).Though PVA is the agent of a kind of emulsifying power higher surface activity, exist in the problem of a small amount of PVA of polyester granules remained on surface, these residual PVA can influence medicine carrying particle and the interaction of physiological environment on every side.The cm-chitosan that utilization of the present invention has biocompatibility replaces PVA to prepare PLA and PLGA microballoon as emulsifying agent, with 0.2% cm-chitosan be the emulsifying agent preparation the PLGA microballoon scanning electronic microscope (SEM) as shown in Figure 2.
With 0.2% cm-chitosan be emulsifying agent preparation the PLGA microballoon surface electronic photoelectron spectrum (XPS) as shown in Figure 3.The characteristic absorbance that does not occur the nitrogen element at the 400eV place illustrates the residual hardly cm-chitosan of particle surface, and promptly the clean proof of the PLGA microsphere surface of preparation is at PLA and the residual hardly cm-chitosan composition of PLGA microsphere surface.Therefore, the present invention utilizes cm-chitosan to prepare the not polyester microsphere of residual emulsifying agent of surface as emulsifying agent.
Application example 2: cm-chitosan can be regulated the pattern and the tissue of vascular cell as biomaterial surface properties-correcting agent
One of key factor of intravascular tissue engineering success is exactly the function that can the cell of vitro culture keep cells in vivo, and the biomaterial surface of cm-chitosan modification can be regulated the pattern and the intercellular tissue of vascular cell.The configuration of surface of chitosan and cm-chitosan characterizes as shown in Figure 4.Compare with the chitosan surface, the cm-chitosan inducing endothelial cell is needle-like, and iuntercellular is linear array.
Cm-chitosan provided by the invention, the cm-chitosan with biocompatibility can replace PVA to be used as emulsifying agent fully and prepare PLA and PLGA microballoon, the residual hardly cm-chitosan composition of microsphere surface.The biomaterial surface of cm-chitosan modification can be regulated the pattern and the intercellular tissue of vascular cell.(as PLA, PLGA) particulate is used for medicine sustained release carrier, and the most frequently used emulsifying agent is polyvinyl alcohol (PVA) and present solvent evaporated method prepares Biodegradable polyester.Though PVA is the agent of a kind of emulsifying power higher surface activity, exist in the problem of a small amount of PVA of polyester granules remained on surface, these residual PVA can influence medicine carrying particle and the interaction of physiological environment on every side.
Description of drawings
Fig. 1 is the relation of surface tension and cm-chitosan concentration;
Fig. 2 is to be the PLGA microballoon of emulsifying agent preparation with 0.2% cm-chitosan;
Fig. 3 is the photoelectron spectrum of PLGA microsphere surface;
Fig. 4 (A) is the F-actin and the nucleus dyeing of chitosan vascular surface endotheliocyte;
Fig. 4 (B) is the F-actin and the nucleus dyeing of cm-chitosan vascular surface endotheliocyte.
Embodiment
1, the novel surfactant cm-chitosan of preparation polyester microsphere
Take by weighing 2g chitosan powder, the NaOH solution soaking of usefulness 25ml 50% 24 hours, solution is squeezed into filter cake shape with chitosan after filtering, and changes in the there-necked flask.Take by weighing a certain amount of Mono Chloro Acetic Acid and be dissolved in the 25ml Virahol, the back adds in the filter cake, stoichiometric number hour under the room temperature, and suction filtration, extruding remove and desolvate.In the gained solid, add 100ml water, stir and make its dissolving, use the HCl of 2.5mol/L to transfer the pH value of solution value, add the 150ml dehydrated alcohol in the supernatant liquor behind the solution centrifugal, have flocculence to precipitate and separate out to neutral.Leave standstill behind the 2h solution centrifugal, flocks is extracted,, be placed on that oven dry can obtain cm-chitosan in 80 ℃ the baking oven with absolute ethanol washing three times.Structural formula is as follows:
2, cm-chitosan is in the tissue surface application in engineering
With 0.2% cm-chitosan is the PLGA microballoon that emulsifying agent can prepare, as Fig. 2.Add the cm-chitosan of the different concns of 1/5 volume in cell culture fluid, the growth of cell is influenced hardly, illustrates that cm-chitosan has better biocompatibility.The cm-chitosan inducing endothelial cell is needle-like, and iuntercellular is linear array, as Fig. 4.
Claims (1)
1, a kind of cm-chitosan is characterized in that in the tissue surface application in engineering cm-chitosan is used as the polyester microsphere that green surfactant prepares surface cleaning, and wherein the cm-chitosan structural formula is:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100949239A CN100484965C (en) | 2005-10-17 | 2005-10-17 | Preparation of carboxymethyl chitosan and its application in tissue surface engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100949239A CN100484965C (en) | 2005-10-17 | 2005-10-17 | Preparation of carboxymethyl chitosan and its application in tissue surface engineering |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1789288A CN1789288A (en) | 2006-06-21 |
| CN100484965C true CN100484965C (en) | 2009-05-06 |
Family
ID=36787433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100949239A Expired - Fee Related CN100484965C (en) | 2005-10-17 | 2005-10-17 | Preparation of carboxymethyl chitosan and its application in tissue surface engineering |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100484965C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101704992B (en) * | 2009-10-29 | 2012-08-22 | 无锡中科光远生物材料有限公司 | Biodegradable and absorbable monodisperse polyester microsphere and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1431229A (en) * | 2003-01-09 | 2003-07-23 | 武汉大学 | Carboxymethyl chitosan, its preparing method and usage |
| CN1556116A (en) * | 2004-01-12 | 2004-12-22 | 中山大学附属第一医院 | Application of o-substituted carboxy methyl chitin as antiadhesion material in surgical operation |
-
2005
- 2005-10-17 CN CNB2005100949239A patent/CN100484965C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1431229A (en) * | 2003-01-09 | 2003-07-23 | 武汉大学 | Carboxymethyl chitosan, its preparing method and usage |
| CN1556116A (en) * | 2004-01-12 | 2004-12-22 | 中山大学附属第一医院 | Application of o-substituted carboxy methyl chitin as antiadhesion material in surgical operation |
Non-Patent Citations (6)
| Title |
|---|
| N,O-羧甲基壳聚糖制备工艺的优化研究. 曾德芳等.武汉理工大学学报，第27卷第6期. 2005 |
| N,O-羧甲基壳聚糖制备工艺的优化研究. 曾德芳等.武汉理工大学学报，第27卷第6期. 2005 * |
| 两步加碱法制备N,O-羧甲基壳聚糖-反应条件对取代度的影响. 钟超等.精细化工，第21卷第5期. 2004 |
| 两步加碱法制备N,O-羧甲基壳聚糖-反应条件对取代度的影响. 钟超等.精细化工，第21卷第5期. 2004 * |
| 壳聚糖和N,O-s羧甲基壳聚糖抑菌活性的研究. 高献礼等.中国食品添加剂，第1期. 2005 |
| 壳聚糖和N,O-s羧甲基壳聚糖抑菌活性的研究. 高献礼等.中国食品添加剂，第1期. 2005 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1789288A (en) | 2006-06-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kumari et al. | Chitin and chitosan: origin, properties, and applications | |
| Xu et al. | Waste-to-wealth: Biowaste valorization into valuable bio (nano) materials | |
| CN101613420B (en) | Preparation method of 2-hydroxypropyltrimethyl ammonium chloride chitosan | |
| Schnepp | Biopolymers as a flexible resource for nanochemistry | |
| CA1158235A (en) | Chitin containing poly-ion complex | |
| CN107099049A (en) | A kind of beta-cyclodextrin cross-linked chitosan multi-porous sorbing material of citric acid-modified and its preparation and application | |
| US5622834A (en) | Method of isolating poly-β-1-4-N-acetylglucosamine from microalgal culture | |
| US4579943A (en) | Cellulose derivative excellent in liquid absorbing property, process for preparing same and structure containing same | |
| Pagliaro et al. | Application of nanocellulose composites in the environmental engineering as a catalyst, flocculants, and energy storages: A review | |
| KR20010091847A (en) | Chitosan and Method of Preparing Chitosan | |
| Cheng et al. | Sustainable cellulose and its derivatives for promising biomedical applications | |
| Lv et al. | Overview of preparation, modification, and application of tunicate-derived nanocellulose | |
| Barhoum et al. | Nanocelluloses as new generation materials: Natural resources, structure-related properties, engineering nanostructures, and technical challenges | |
| CN109908876A (en) | A kind of preparation method and application of the chitosan-based bionical attractive gel of polysaccharide nano microcrystalline doping | |
| Liu et al. | Reinforced chitosan beads by chitin nanofibers for the immobilization of β-glucosidase | |
| CN111471193B (en) | Dialdehyde polysaccharide nanoparticle crosslinked collagen hydrogel and preparation method thereof | |
| CN113145080A (en) | Preparation method of TEMPO oxidized holocellulose-chitosan adsorption material, prepared adsorption material and application | |
| CN100484965C (en) | Preparation of carboxymethyl chitosan and its application in tissue surface engineering | |
| Kamalesh et al. | A critical review on sustainable cellulose materials and its multifaceted applications | |
| CN100369935C (en) | Process for preparing carboxymethyl cellulose crosslinked amide derivative | |
| CN105732842B (en) | A kind of high bioactivity chitin and preparation method thereof | |
| Zhang et al. | Hydrogels based on seafood chitin: From extraction to the development | |
| Ciriminna et al. | Enhanced polysaccharide nanofibers via oxidation over Silia Cat TEMPO | |
| CN111116996B (en) | Cellulose aerogel modified by surfactant and preparation method thereof | |
| CN102219844B (en) | Method for preparing water-insoluble fibroin nanometer microcrystal powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090506 Termination date: 20111017 |