CN100484962C - Method for preparing Codonopsis pilosula polysaccharide extractive as Codonopsis pilosula active ingredient - Google Patents

Abstract

A process for extracting the codonopsinose used to prepare the codonopsinose medicines in the form of infusion, capsule, syrup, or oral liquid includes cutting the pilose asiabell root, decocting 2-3 times, collecting filtered decoction, vacuum concentrating, cooling, centrifugal separation, drying the deposit, and pulverizing.

Description

The preparation method of Radix Codonopsis asiabell effective ingredient polyoses extract

Technical field

The present invention is a kind of Chinese medicinal materials Radix Codonopsis effective ingredient preparation method of extract.

Background technology

The Chinese medicinal materials Radix Codonopsis is the first-selected invigorating the spleen and replenishing QI of traditional tcm clinical, invigorating the spleen benefit lung, strengthens the body resistance to consolidate the constitution, strengthening by means of tonics and antidotal key medicine, be applicable to spleen-lung Qi deficiency all cards, many Chinese and overseas scholars have carried out extensive studies with regard to its kind, plant, pharmacology and aspect such as clinical, confirm that Radix Codonopsis has many-sided pharmacological action, for theory of traditional Chinese medical science has found scientific basis, utilization modern science means have been carried out fundamental research to Radix Codonopsis, have therefrom found the active substance Radix Codonopsis polysaccharide.In the single dangshen oral liquid body preparation that nineteen seventies once emerged, adopted the alcohol precipitation process preparation,, caused effective ingredient to be lost because no corresponding detecting method can't detect; The Chinese patent medicine compound preparation that contains the Radix Codonopsis composition also all is to adopt water extract-alcohol precipitation preparation technology, all causes losing of active principle.

Aspect medicinal application, be the compatibility that is mainly used to prescription and some Chinese patent medicines with medicine materical crude slice, oral liquid is because of the preparation technology science reason market of fading out such as affect the treatment not.The further development and use of Shanxi authentic medicinal herbs have been influenced.

Summary of the invention

The present invention is directed to the technical problem that prior art exists, a kind of decoction liquor concentrated solution is provided, prepare Radix Codonopsis polysaccharide extract with the method for depositing in water.

The present invention realizes that by following scheme the preparation method of Radix Codonopsis asiabell effective ingredient polyoses extract is characterized in that its preparation method may further comprise the steps:

A, dedusting are cleaned: the raw material Radix Codonopsis is removed impurity and the medicine that goes mouldy, flushing with clean water;

B, chopping: the section that Radix Codonopsis is cut into 2-4CM;

C, decoction: with the Radix Codonopsis section that cuts, put into to boil and propose the cylinder decoction, add pure water 7-10 doubly, decoct 2-3 time, filtered in each 2-3 hour, merging filtrate promptly gets feed after the filter residue and drying processing;

D, concentrated: the filtrate decompression of merging is concentrated into semi-fluid, proportion 1.25-1.26, and temperature is placed cooling for 70 ℃-80 ℃;

E, centrifugation: will place the refrigerative concentrated solution, centrifugation gets throw out and the mother liquor throw out is handled through following method: throw out is washed secondary with pure water, add pure water at every turn and stir evenly centrifugation by throw out weight in wet base 1/3-1/4, in 60 ℃ of-80 ℃ of dryings, getting yellow powdery throw out is Radix Codonopsis polysaccharide extract, and Radix Codonopsis polysaccharide content is more than 60%;

F, mother liquor are handled: merge three times separating obtained mother liquor, concentrated proportion is 1.25-1.26, and temperature is at 70 ℃-80 ℃, and Radix Codonopsis polysaccharide content is more than 2%.

The present invention is solving the quality standard of measuring the activeconstituents Radix Codonopsis polysaccharide and is confirming that Radix Codonopsis asiabell effective ingredient polysaccharide content reaches further development research on the basis 10% or more.Adopt method of the present invention to prepare Radix Codonopsis polysaccharide extract's purity height, the volume I directly prepares capsule, electuary, syrup or oral liquid.

Preparation of the present invention has Radix Codonopsis polysaccharide capsule or Radix Codonopsis polysaccharide syrup or Radix Codonopsis polysaccharide electuary or oral liquid.The Radix Codonopsis polysaccharide capsule with Radix Codonopsis polysaccharide extract, is loaded in capsule for medicine, packs promptly.The syrupy preparation of Radix Codonopsis polysaccharide is regulated content with the mother liquor of Radix Codonopsis polysaccharide more than 2% with Radix Codonopsis polysaccharide extract, and its Radix Codonopsis polysaccharide content is reached more than 5%, is distributed into bag or bottle, promptly gets the Radix Codonopsis polysaccharide syrup.

Radix Codonopsis polysaccharide electuary of the present invention, its prescription total weight percent is:

The 10%-50% of major ingredient Radix Codonopsis polysaccharide extract

Auxiliary material fructose, polysaccharide or sucrose one or more: 50-90%

With major ingredient and auxiliary materials and mixing, packing promptly gets the Radix Codonopsis polysaccharide electuary

The Radix Codonopsis polysaccharide oral liquid of Radix Codonopsis polysaccharide extract's preparation, it is mixed with by following anharmonic ratio,

Major ingredient: the 10-30% of Radix Codonopsis polysaccharide extract

Auxiliary material: fructose, glucose or sucrose one or more and water, 70-90%, with major ingredient and auxiliary materials and mixing, packing promptly gets the Radix Codonopsis polysaccharide oral liquid.

Raw materials used Chinese medicinal materials Radix Codonopsis among the present invention, medicine source abundance and quality standard advanced person provide reliable basis for the research and development Radix Codonopsis.Because the effective constituent quality standard of Radix Codonopsis system adopts colleges and universities' liquid phase quantitative, to fully take into account in the formulation development plan whether standard test has interference to quality, develop the Radix Codonopsis medicine series for this reason and selected electuary, capsule and syrup or oral liquid.

The present invention makes full use of the natural resources, and utilizes the dregs of a decoction (component of the dregs of a decoction is kinds more than 10 such as protein content 8%, crude fiber content 21%, trace elements iron, manganese, zinc, copper, selenium) to develop comparatively ideal feed in the time of comprehensive exploitation Radix Codonopsis medicine series.

Beneficial effect of the present invention:

1. improved the Radix Codonopsis polysaccharide extraction process, replaced alcohol precipitation, saved ethanol, reduced cost, improved the purity and the content of Radix Codonopsis polysaccharide with depositing in water.

2. the raw medicinal material Radix Codonopsis has clear and definite effective ingredient quality standard

3. the raw medicinal material Radix Codonopsis has competent medicine source

4. medicine series all has strict quality standard, adopts advanced high-pressure liquid phase quantitative, and the inner quality of medicine series obtains the assurance of science

5. pure natural medical does not add sanitas and crude drug component in addition

6. the selection space of multi-form is provided for clinical application, has made things convenient for each colony's medication particularly old man and child

7. the comprehensive exploitation product dregs of a decoction is characterized in that protein content 8%, kind surplus the crude fiber content 21%, trace elements iron, manganese, zinc, copper etc. 10.

Embodiment

Embodiment 1

Radix Codonopsis effective ingredient Radix Codonopsis polysaccharide extract, this extract can obtain with following method, get quality standard clearly Radix Codonopsis starting material 100Kg be chopped into the segment of 2-3cm, the pure water that adds 1000kg, decocted 2-3 time each 2-3 hour, and filtered merging filtrate, clarification, be evaporated to semi-fluid, proportion 1.25-1.26 temperature is placed cooling for 70 ℃-80 ℃.Centrifugation gets throw out and mother liquor, and it is the 1/3-1/4 of moist precipitate thing that throw out adds the weight of washing twice water, stirs evenly, centrifugation, throw out is in 60-80 ℃ of drying, get yellow powder shape Radix Codonopsis polysaccharide, three times mother liquor merges, and measures Radix Codonopsis polysaccharide content more than 2%.

Embodiment 2

The capsular preparation of Radix Codonopsis: get the Radix Codonopsis polysaccharide extract powder of content more than 80%, load in capsule for medicine, pack, every 0.5 gram (be equivalent to raw medicinal material 3 gram), everyone every day 1-2 time of being grown up, 3-4 grain at every turn.

Embodiment 3

The preparation of Radix Codonopsis electuary: get content 80% Radix Codonopsis polysaccharide powder 100 grams, sucrose 400 gram mixings, pack (aluminium foil film), every bag 10 gram (being equivalent to crude drug Radix Codonopsis 15 grams) is grown up each 1 bag, and 1-2 time on the 1st, children are cut down according to the circumstance.

Embodiment 4

The syrupy preparation of Radix Codonopsis: get Radix Codonopsis polysaccharide content 5% centrifuge mother liquor 30ml, in the packing aluminium foil bag, be grown up each 1 bag, 1-2 time on the 1st, children are cut down according to the circumstance.

Claims (1)

1, a kind of preparation method of Radix Codonopsis asiabell effective ingredient polyoses extract, its preparation method may further comprise the steps:

A, dedusting are cleaned: the raw material Radix Codonopsis is removed impurity and the medicine that goes mouldy, flushing with clean water;

B, chopping: the section that Radix Codonopsis is cut into 2-4CM;

C, decoction: with the Radix Codonopsis section that cuts, put into to boil and propose the cylinder decoction, add pure water 7-10 doubly, decoct 2-3 time, filtered in each 2-3 hour, merging filtrate promptly gets feed after the filter residue and drying processing;

D, concentrated: the filtrate decompression of merging is concentrated into semi-fluid, proportion 1.25-1.26, and temperature is placed cooling for 70 ℃-80 ℃;

E, centrifugation: will place the refrigerative concentrated solution, centrifugation gets throw out and mother liquor, throw out is handled through following method: throw out is washed secondary with pure water, add pure water at every turn and stir evenly centrifugation by throw out weight in wet base 1/3-1/4, in 60 ℃ of-80 ℃ of dryings, getting yellow powdery throw out is Radix Codonopsis polysaccharide extract, and Radix Codonopsis polysaccharide content is more than 60%;

F, mother liquor are handled: merge three times separating obtained mother liquor, concentrated proportion is 1.25-1.26, and temperature is at 70 ℃-80 ℃, and Radix Codonopsis polysaccharide content is more than 2%.