CN100463971C - Color developing culture medium, detecting kit and detecting method for vibrio parahaemolyticus - Google Patents
Color developing culture medium, detecting kit and detecting method for vibrio parahaemolyticus Download PDFInfo
- Publication number
- CN100463971C CN100463971C CNB2006101240756A CN200610124075A CN100463971C CN 100463971 C CN100463971 C CN 100463971C CN B2006101240756 A CNB2006101240756 A CN B2006101240756A CN 200610124075 A CN200610124075 A CN 200610124075A CN 100463971 C CN100463971 C CN 100463971C
- Authority
- CN
- China
- Prior art keywords
- culture medium
- color developing
- vibrio parahemolyticus
- liquid
- enrichment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to color developing culture medium, detecting kit and detection method for Vibrio parahaemolyticus, and belongs to the field of food microbe safety monitoring technology. The detecting kit consists of color developing culture medium comprising bacterial specific enzyme in 0.05-0.95 g and mixed color developing substrate Y-glucoside, enrichment liquid A of pancreatic casitone liquid culture medium and enrichment liquid B comprising sodium chloride, polymyxin and broth. The detection method includes the enrichment culture of the sample in enrichment liquid A and enrichment liquid B, the culture of the two parts of enrichment cultured liquid in the color developing culture medium, and judging whether to exist Vibrio parahaemolyticus according to whether to have smooth raised purple colony of 2-3 mm diameter. The present invention has low cost, high detection sensitivity, short detection period and other advantages.
Description
[technical field]
This paper relates to a kind of microorganism color developing culture medium, detection kit and detection method, relates in particular to Vibrio parahemolyticus color developing culture medium, detection kit and detection method, belongs to food microorganisms safety monitoring field.
[background technology]
" bread is the staff of life ", food are the basic substances that the mankind depend on for existence, and food safety is the significant problem that concerns human health and social development.In recent years, both at home and abroad the malignant event of food safety constantly takes place, and especially the food origin disease that causes with Salmonellas, enterohemorrhagic Escherichia coli, Listeria monocytogenes, Vibrio parahemolyticus etc. is the most outstanding.According to statistics, developed country has 1/3 people to suffer from food origin disease every year approximately, and the U.S. has 7,600 ten thousand routine food origin disease patients every year approximately, and food origin disease has become the problem of generally being concerned about both at home and abroad.
Vibrio parahemolyticus (Vibrio parahaemolyticus is called for short VP) is one of human common important food-borne pathogens, extensively is present in the coastland of countries in the world, is a kind of halophilism bacterium.Human consumer Duo Yin has eaten the sea-food that pollutes this bacterium such as fish, shrimp, shellfish, crab etc. and has caused food poisoning.The ratio that the food poisoning that is caused by Vibrio parahemolyticus accounts in bacterial food poisoning is very high, is only second to intestinal bacteria, Salmonellas and staphylococcus.China's Coastal Areas all has the report of vibrio parahaemolytisus poisoning every year, toxicity symptom has diarrhoea, watery stool, abdominal cramps, feels sick, vomiting etc., cause very big threat to human health, bring very big negative impact also for simultaneously the economy and the foreign trade of China.
Be the effectively generation and the diffusion of control cause of disease, detection means accurately and timely is the key that prevention and control Vibrio parahemolyticus are propagated.At present, the detection method of Vibrio parahemolyticus is a lot, traditional detection method needs a plurality of steps such as separation and Culture, microscopy observation, biochemical identification, halophilism test, sense cycle is long, the about 4-7d of whole process, and complicated operation can not satisfy the real needs of pathogenic bacterium rapid detection in the food, and can influence Vibrio parahemolyticus detect on traditional Sulfothiorine-Trisodium Citrate-cholate-sucrose agar (TCBS) flat board when other assorted bacterium such as vibrio alginolyticus, vibrio cholerae exist.In recent years, the application of methods such as polymerase chain reaction (PCR), immunology makes the rapid detection of Vibrio parahemolyticus that very great development arranged.But also there is certain defective in these technology, and are higher as technical requirements, and the operation of equipment complexity needs the technical professional to operate, and the testing cost costliness etc.PCR method need be carried out the bacterium enrichment earlier, can not distinguish viable bacteria and dead bacterium, is easy to generate false positive; Immunological method also needs to prepare highly purified antibody, so these technology are difficult to satisfy business and government supervision department to large quantities of food samples detection needs.
The good company of present French Kerma (unit of kinetic energy) has developed the color developing culture medium of Vibrio parahemolyticus, but costs an arm and a leg, and auxiliary products and reagent need import, and it is very high to detect cost, and therefore, more domestic feeler mechanisies of basic unit seldom adopt.
[summary of the invention]
The present invention aims to provide a kind of selectivity height, sense cycle is short, cost is low, workable, be applicable to Vibrio parahemolyticus color developing culture medium, detection kit and the detection method of handling big flux sample.
Described Vibrio parahemolyticus color developing culture medium, its prescription is: peptone 10～15g, yeast powder 3～5g, NaCl 9～11g, sucrose 18～20g, monose 0.11～0.13g, Trisodium Citrate 9～11g, Sulfothiorine 9～11g, cholate 5～8g, mixing chromogenic substrate Y-glucoside 0.05～0.95g, agar 12～15g, NaCO
31～1.5g.
Above-mentioned color developing culture medium is preferably filled a prescription and is: peptone 15g, yeast powder 3g, NaCl 10g, sucrose 20g, monose 0.11g, Trisodium Citrate 10g, Sulfothiorine 10g, cholate 8g, mixing chromogenic substrate Y-glucoside 0.35g, agar 12g, NaCO
31g.
Described Vibrio parahemolyticus detection kit, it consists of: above-mentioned Vibrio parahemolyticus color developing culture medium, enrichment liquid A and B.
Wherein, enrichment liquid A is the trypticase liquid nutrient medium (TSB) of sterilising treatment;
Enrichment liquid B is an autoclaved sodium-chlor PXB meat soup (3.5%NaCL PolyB).
Described Vibrio parahemolyticus detection method, its concrete steps are:
(1) the preparation colour developing is dull and stereotyped: add 77～98g color developing culture medium dry powder in every 1000mL water, boil 1～2min after mixing, wait to be chilled to 40～50 ℃, fall dull and stereotyped, standby;
(2) one steps increased bacterium (non-selective increase bacterium): sample is placed enrichment liquid A, mix back 37 ℃ and cultivate 6～8h;
(3) two steps increased bacterium (selective enrichment): get a step enrichment liquid 1mL and join among the 9mL enrichment liquid B, mix back 37 ℃ and cultivate 15～17h;
(4) inoculation culture: on two steps enrichment liquid inoculation colour developing flat board, cultivate 18～24h for 37 ℃;
(5) interpretation of result: smooth, as to omit projection, diameter 2-3mm, neat in edge purple bacterium colony occurs as if dull and stereotyped the going up of colour developing, illustrate that there is Vibrio parahemolyticus in this sample.
The present invention is directed to deficiency of the prior art,, utilize the bacterium specific enzymes that it is carried out rapid detection from biochemical angle.At first, on the basis of analyzing Vibrio parahemolyticus specificity biochemical reaction, filter out the bacterium specific enzymes, then by on isolation medium, adding the chromogenic substrate of bacterium specific enzymes, develop color developing culture medium, directly just can make evaluation to bacterial strain according to colony colour, combination simultaneously is the sample pretreatment technology efficiently, set up specificity colour developing biochemistry detection method, and develop supporting test kit, be used for quick diagnosis and the monitoring of Vibrio parahemolyticus, can save greatly and detect cost and time, have application prospect widely.
The present invention has developed the color developing culture medium of Vibrio parahemolyticus by screening Vibrio parahemolyticus specific enzymes and chromogenic substrate, has overcome that existing color developing culture medium costs an arm and a leg and shortcoming such as traditional substratum poor selectivity; And the sample process technology by the various standard methods of contrast, the Vibrio parahemolyticus of the having set up a cover system biochemical method for quick that develops the color has improved detection efficiency; The Vibrio parahemolyticus of being set up develop the color biochemical method for quick and test kit can carry out comprehensively Vibrio parahemolyticus in food and the environment, system, detect accurately and identify, for microbial rapid detection provides new approach.
Test kit of the present invention configuration is simple, and detection method detects sensitive high, and the cycle is short, workable, be applicable to the big flux samples of processing, be easy to industrialization production, can wide popularization and application to fields such as food sanitation, environmental monitorings.
[embodiment]
Embodiment 1: the specific checking of Vibrio parahemolyticus color developing culture medium of the present invention
1, the preparation of Vibrio parahemolyticus color developing culture medium: get peptone 15g, yeast powder 3g, NaCl 10g, sucrose 20g, monose 0.11g, Trisodium Citrate 10g, Sulfothiorine 10g, cholate 8g, mix chromogenic substrate Y-glucoside (U.S. sigma company) 0.35g, agar 12g, NaCO3 1g, water 1000mL, mixing post-heating to agar dissolves fully, boil 1～2min, transfer pH 8.6 ± 0.2, to be cooled to 40～50 ℃, fall dull and stereotyped, standby.
2, inoculation: with Vibrio parahemolyticus (Vibrio parahaemolyticus), vibrio cholerae (Vibriocholera), vibrio alginolyticus (Vibrioalginolyticus), Vibrio vulnificus (Vibrio vulnificus), Vibrio mimicus (Vibriomimicus) is at the 3.5%NaCl nutrient agar medium, intestinal bacteria (Eschetichiacoli), Salmonellas (Salmonella) is at nutrient agar medium, Listeria monocytogenes (Listeriamonocytogenes) is behind brain heart infusion agar recovery 24h, the above-mentioned color developing culture medium flat board of streak inoculation is cultivated 18～24h for 36 ℃ respectively.
3, result and analysis: on the colour developing flat board of Vibrio parahemolyticus, the purple bacterium colony occurs; The blue-greenish colour bacterium colony all appears on the colour developing flat board of vibrio cholerae, Vibrio vulnificus, Vibrio mimicus, white colony appears on the colour developing flat board of vibrio alginolyticus, all do not have the growth phenomenon on the colour developing flat board of intestinal bacteria, Salmonellas, Listeria monocytogenes, illustrate that color developing culture medium has higher specificity to Vibrio parahemolyticus.
Embodiment 2: Vibrio parahemolyticus color developing culture medium of the present invention sensitivity and specific checking
1, the preparation of Vibrio parahemolyticus color developing culture medium: get peptone 15g, yeast powder 3g, NaCl 10g, sucrose 20g, monose 0.11g, Trisodium Citrate 10g, Sulfothiorine 10g, cholate 8g, mix chromogenic substrate Y-glucoside (U.S. sigma company) 0.35g, agar 12g, NaCO3 1g, water 1000mL, mixing post-heating to agar dissolves fully, boil 1～2min, transfer pH 8.6 ± 0.2, to be cooled to 40～50 ℃, fall dull and stereotyped, standby.
2, inoculation:
In the 3.5%NaCl nutrient agar medium behind the recovery 24h, transfering loop picking 1 ring joins in 10mL 0.85% physiological saline and is made into stoste, carries out 10 times of gradient dilutions then, gets 10 with Vibrio parahemolyticus
-4-10
-6Each 1mL of concentration bacterium liquid, the above-mentioned color developing culture medium flat board of separate application, traditional TCBS flat board and Kerma (unit of kinetic energy) are praised the Vibrio parahemolyticus color developing culture medium that company produces, and cultivate 18～24h for 36 ℃.
Vibrio parahemolyticus, vibrio cholerae, vibrio alginolyticus, Vibrio vulnificus, Vibrio mimicus, intestinal bacteria, Salmonellas, Listeria monocytogenes are mixed, carry out gradient dilution, get 10
4-10
5The cfu/mL mixed bacteria liquid, the color developing culture medium flat board of separate application step 1 preparation is observed.
3, result and analysis:
Pure bacterium liquid is coated with each dull and stereotyped result such as table 1, colony number between the 30-300 is carried out variance analysis, the Vibrio parahemolyticus recall rate that shows the Vibrio parahemolyticus color developing culture medium that Vibrio parahemolyticus color developing culture medium of the present invention and traditional TCBS flat board, the good company of Kerma (unit of kinetic energy) produce does not have significant difference (P〉0.05), and three kinds of substratum can reach identical detectability.
The colony number (mean value Sd) of table 1 Vibrio parahemolyticus on each substratum
| 10 -4 | 10 -5 | 10 -6 | |
| Color developing culture medium of the present invention | How can not count | 140±2.03 | 5±1.97 |
| Color developing culture medium (Kerma (unit of kinetic energy) is praised company) | How can not count | 144±1.85 | 9±2.0 |
| TCBS | How can not count | 152±2.47 | 13±2.18 |
On the mixed bacteria liquid spread plate, Vibrio parahemolyticus still is special purple bacterium colony, and other vibrios present blueness or white colony.Show that other vibrios do not influence the detection of Vibrio parahemolyticus substantially, color developing culture medium is to the specificity height of Vibrio parahemolyticus.
Embodiment 3: the comparison of Vibrio parahemolyticus detection method of the present invention and national standard detection method (abbreviation National Standard Method)
Gather a large amount of sea-foods, fresh-water fishes etc. from market, adopt National Standard Method (GB/T4789.7-2003 respectively, microbiological test of food hygiene Vibrio parahemolyticus check) and detection method of the present invention Vibrio parahemolyticus is detected, detect positive sample and verify the sensitivity of two kinds of detection methods of comparison with French Mei Liai Bacteria Identification system-API reagent strip.
(1) National Standard Method: sample-sodium-chlor PXB meat soup (or sodium-chlor Viola crystallina) increases bacterium 24h--streak inoculation TCBS flat board-blue-greenish colour bacterium colony--biochemical identification.
(2) detection method of the present invention: increase bacterium 6h-sodium-chlor PXB meat soup selective enrichment 16h-streak inoculation color developing culture medium-direct viewing of the present invention before the TSB meat soup of sample-contain 3.5% NaCl and have or not the purple bacterium colony.
National Standard Method and detection method of the present invention by Vibrio parahemolyticus in 325 parts of markets and the representative food such as supermarket sea-food, freshwater fish, result such as table 2, illustrate with Vibrio parahemolyticus detection method of the present invention to have higher recall rate, and detection time is shorter than National Standard Method.
| Method | Recall rate | Detection time |
| Detection method of the present invention | 14.1％ | 24～46h |
| National Standard Method | 12.3％ | 4～7d |
Embodiment 4: the simulation test that Vibrio parahemolyticus detects in the flesh of fish
1, the preparation colour developing is dull and stereotyped
Take by weighing 89.36g color developing culture medium dry powder, add 1000mL water, boil 1～2min, wait to be chilled to 40～50 ℃, fall dull and stereotyped, standby.
2, a step increases bacterium
Take by weighing 25g flesh of fish sample, 1～10cfu Vibrio parahemolyticus is added in the 25g sample, simulation actual sample mixings 2h, aseptic technique is put into the triangular flask that fills 225mL TSB enrichment liquid with sample, and 37 ℃ of one step increases bacterium (non-selective increase bacterium) cultivation 6h.
3, two steps increased bacterium
Get 1mL sample one step enrichment liquid and join and carried out for two steps in the 9mL sodium-chlor PXB meat soup and increase bacterium, cultivate 16h for 37 ℃.
4, inoculation culture
Get 1 ring, two steps enrichment liquid, the streak inoculation color developing culture medium is cultivated 18～24h, observed and recorded bacterium colony size, color and form for 37 ℃.
5, the result observes and analyzes
Present smooth, as to omit projection, diameter 2-3mm, neat in edge purple bacterium colony on the color developing culture medium.In the testing process, a step increases bacterium needs 6h, and two steps increased bacterium 16h, the dull and stereotyped 18～24h that cultivates of colour developing, the longest 46h that needs of positive findings time.Detect detection sensitivity and can reach 1～10cfu.
Embodiment 5: the detection of Vibrio parahemolyticus in the shellfish
1, the preparation colour developing is dull and stereotyped
Take by weighing 89.36g color developing culture medium dry powder, add 1000mL water, boil 1～2min, wait to be chilled to 40～50 ℃, fall dull and stereotyped, standby.
2, a step increases bacterium
Under aseptic condition, take by weighing 25g shellfish meat, shred, join in the triangular flask that fills 225mL TSB enrichment liquid, 37 ℃ of one step increases bacterium (non-selective increase bacterium) and cultivates 6h.
3, two steps increased bacterium
Get 1mL sample one step enrichment liquid and join and carried out for two steps in the 9mL sodium-chlor PXB meat soup and increase bacterium (selective enrichment), cultivate 16h for 37 ℃.
4, inoculation culture
Transfering loop is got 1 ring, two steps enrichment liquid, and the streak inoculation color developing culture medium is cultivated 18-24h, observed and recorded colony colour and form for 37 ℃.Compare the comparative analysis detected result with Vibrio parahemolyticus reference culture streak inoculation color developing culture medium.
4, interpretation of result
Colour developing presents the purple bacterium colony of purple, smooth, neat in edge, diameter 2-3mm on the flat board, and colony colour conforms to reference culture with form, illustrates in the shellfish sample to have Vibrio parahemolyticus.
Claims (4)
1. a Vibrio parahemolyticus color developing culture medium is characterized in that the prescription of described substratum is: peptone 10～15g, yeast powder 3～5g, NaCl 9～11g, sucrose 18～20g, monose 0.11～0.13g, Trisodium Citrate 9～11g, Sulfothiorine 9～11g, cholate 5～8g, mixing chromogenic substrate Y-glucoside 0.05～0.95g, agar 12～15g, NaCO
31～1.5g.
2. Vibrio parahemolyticus color developing culture medium as claimed in claim 1 is characterized in that the prescription of described substratum is: peptone 15g, yeast powder 3g, NaCl 10g, sucrose 20g, monose 0.11g, Trisodium Citrate 10g, Sulfothiorine 10g, cholate 8g, mixing chromogenic substrate Y-glucoside 0.35g, agar 12g, NaCO
31g.
3. a Vibrio parahemolyticus detection kit is characterized in that described test kit comprises
Enrichment liquid A: the trypticase liquid nutrient medium,
Enrichment liquid B: sodium-chlor PXB meat soup,
And color developing culture medium as claimed in claim 1 or 2.
4. method of using test kit as claimed in claim 3 to detect Vibrio parahemolyticus is characterized in that may further comprise the steps:
(1) the preparation colour developing is dull and stereotyped: add 77～98g color developing culture medium in every 1000mL water, boil 1～2min after the mixing, wait to be chilled to 40～50 ℃, fall dull and stereotyped, standby;
(2) one steps increased bacterium: sample is placed enrichment liquid A, mix back 37 ℃ and cultivate 6～8h;
(3) two steps increased bacterium: get a step enrichment liquid 1mL and join among the 9mL enrichment liquid B, mix back 37 ℃ and cultivate 15～17h;
(4) inoculation culture: on two steps enrichment liquid inoculation colour developing flat board, cultivate 18～24h for 37 ℃;
(5) interpretation of result: smooth, as to omit projection, diameter 2-3mm, neat in edge purple bacterium colony occurs as if dull and stereotyped the going up of colour developing, illustrate that there is Vibrio parahemolyticus in this sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101240756A CN100463971C (en) | 2006-12-08 | 2006-12-08 | Color developing culture medium, detecting kit and detecting method for vibrio parahaemolyticus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101240756A CN100463971C (en) | 2006-12-08 | 2006-12-08 | Color developing culture medium, detecting kit and detecting method for vibrio parahaemolyticus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1974784A CN1974784A (en) | 2007-06-06 |
| CN100463971C true CN100463971C (en) | 2009-02-25 |
Family
ID=38125130
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006101240756A Expired - Fee Related CN100463971C (en) | 2006-12-08 | 2006-12-08 | Color developing culture medium, detecting kit and detecting method for vibrio parahaemolyticus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100463971C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102363802B (en) * | 2011-10-08 | 2013-08-07 | 广州绿洲生化科技股份有限公司 | Vibrio parahaemolyticus chromogenic medium and rapid detection card for the same |
| CN102586452B (en) * | 2012-03-12 | 2013-09-11 | 上海海洋大学 | Vibrio parahemolyticus detection kit and detection method thereof |
| CN102703566A (en) * | 2012-05-31 | 2012-10-03 | 上海市疾病预防控制中心 | Plate kit for identifying vibrio parahemolyticus toxigenic strain, and preparation and using methods for plate kit |
| CN102888440A (en) * | 2012-09-28 | 2013-01-23 | 中国水产科学研究院东海水产研究所 | Method for quickly determining total amount of Vibrio parahaemolyticus in aquatic product |
| CN103013822B (en) * | 2012-12-24 | 2014-07-16 | 山东省海洋生物研究院 | Fast drug sensitivity detection kit for aquatic product vibrio |
| CN104293880A (en) * | 2014-10-28 | 2015-01-21 | 天津大学 | Seawater selective chromogenic culture media for VP (Vibrio Parahaemolyticus) and authenticating and counting method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1743842A (en) * | 2004-09-03 | 2006-03-08 | 中国科学院沈阳应用生态研究所 | A kind of thin-layer chromatography coloration method |
-
2006
- 2006-12-08 CN CNB2006101240756A patent/CN100463971C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1743842A (en) * | 2004-09-03 | 2006-03-08 | 中国科学院沈阳应用生态研究所 | A kind of thin-layer chromatography coloration method |
Non-Patent Citations (6)
| Title |
|---|
| CHROMagar沙门菌显色培养基的应用和检测效果评估. 许学斌等.中国预防医学杂志，第6卷第3期. 2005 |
| CHROMagar沙门菌显色培养基的应用和检测效果评估. 许学斌等.中国预防医学杂志，第6卷第3期. 2005 * |
| 科码嘉培养基在病原微生物分离中的应用. 龚元伟等.微生物学杂志，第26卷第5期. 2006 |
| 科码嘉培养基在病原微生物分离中的应用. 龚元伟等.微生物学杂志，第26卷第5期. 2006 * |
| 评估CHRO Mgaar金黄色葡萄球菌显色培养基在化妆品检测中的应用效果. 钱，茵等.香料香精化妆品，第4期. 2005 |
| 评估CHRO Mgaar金黄色葡萄球菌显色培养基在化妆品检测中的应用效果. 钱，茵等.香料香精化妆品，第4期. 2005 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1974784A (en) | 2007-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102424832B (en) | Chromogenic medium used for detecting esherichia coli O157:H7 | |
| CN100463971C (en) | Color developing culture medium, detecting kit and detecting method for vibrio parahaemolyticus | |
| CN102311990B (en) | Chromogenic medium of coliform group and quick detection card thereof | |
| CN102433373B (en) | Salmonella characteristic chromogenic liquid nutrient medium, preparation method thereof and rapid detection method of salmonella | |
| WO1996040861A1 (en) | Microbiological media for isolation and identification of enteric pathogens such as e. coli and salmonella | |
| CN102943113B (en) | Loop-mediated isothermal amplification detection primer groups of Escherichia coli 0157, detection method and reagent kit | |
| CN103290094B (en) | A kind of streptococcus aureus color developing culture medium and testing plate thereof | |
| Ottaviani et al. | Occurrence and characterization of Aeromonas spp. in mussels from the Adriatic Sea | |
| JP2008545382A (en) | Chromogenic plating medium for identification of Enterobacter sakazaki | |
| CN101186891A (en) | Salmonella color culture medium, detection kit an detection method | |
| CN101497915B (en) | Reagent and method for fast detecting Staphylococcus aureus | |
| CN102827918A (en) | Chromogenic medium for detecting salmonella | |
| CN102146429B (en) | Vibrio alginolyticus selective differential medium | |
| CN101423862A (en) | Enterobacter sakazaii colour development culture medium, detection kit and detection method | |
| CN101851676B (en) | Composite enrichment medium of salmonella, shigella and staphylococcus aureus, preparation method and application thereof | |
| CN105039202B (en) | A kind of salmonella, Listeria monocytogenes and the compound selective medium for increasing bacterium of vibrio parahaemolytious and preparation method thereof | |
| CN103614452A (en) | Composite culture medium and method for quickly detecting total bacterial count by using same | |
| CN101215597A (en) | Bacillus cereus specificity color biochemical fast detection kit and detection method | |
| CN101970682A (en) | Method for detecting and/or identifying clostridium difficile | |
| CN101402997B (en) | Reagent kit and method for detecting bacillus cereus with loop mediated isothermality amplification method | |
| CN101555514A (en) | Vibrio mimicus chromogenic culture medium | |
| CN102914649B (en) | Kit for rapidly detecting staphylococcus aureus in quick-frozen food and detection method thereof | |
| CN105861623A (en) | Chromogenic culture medium for detecting Enterobacter sakazakii | |
| CN104388526A (en) | Staphylococcus aureus selective chromogenic culture medium and test paper thereof | |
| CN100453638C (en) | Culture medium with simultaneoulsy enriched salmonella, colibacillus and Staphylococcus aureus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090225 Termination date: 20131208 |