CN100462013C - Artificial feeding milk for sterilized rat - Google Patents
Artificial feeding milk for sterilized rat Download PDFInfo
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- CN100462013C CN100462013C CNB2005100604623A CN200510060462A CN100462013C CN 100462013 C CN100462013 C CN 100462013C CN B2005100604623 A CNB2005100604623 A CN B2005100604623A CN 200510060462 A CN200510060462 A CN 200510060462A CN 100462013 C CN100462013 C CN 100462013C
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Abstract
The present invention discloses germ-free rat artificial feeding milk. According to the postnatal age in days of suckling mouse it can be divided into 0-10-day group and 11-21-day group. Said artificial feeding milk for germ-free rat is formed from raw materials of rabbit milk, cow milk, rat serum, lactose, amino acids, vitamins, milk powder, egg and protein powder, etc.
Description
Technical field
The present invention relates to animal artificial feeding dairy products, especially relate to a kind of artificial feeding milk for sterilized rat.
Background technology
Artificial feeding milk for sterilized rat is meant that the newborn rat that caesarean birth under the aseptic condition is obtained carries out the used artificial milk of artificial suckling.Rat milk is difficult to obtain, and will guarantee germ-free condition, must carry out autoclaving to the milk of the newborn rat of feeding.Particularly vitamin destruction is very big but autoclaving is to nutritional labeling, so just needs to select suitable animal as the milk source, and replenishes ruined composition.
That has announced at present has 4 kinds of rat artificial feeding breasts, and see Table 1 concrete the composition:
Table 1
Above-mentioned 4 kinds of prescriptions are tested, and following problem is found in the test back:
1. fill a prescription 1, prescription 2, composition is too complicated, some compositions are optional, used milk is milk or milk powder, so protein content is on the low side; And artificial milkiness shape is rarer behind the autoclave sterilization, only is applicable to that gavage feeds, and is not suitable for the method for sucking automatically.Very easily will suckle to suck in the lung and cause foreign body pneumonia because suckling mouse sucks rarer breast, cause suckling mouse growth retardation or death;
2. 3 protein contents of filling a prescription are than the content height of prescription 1 and 2, but composition is still very complicated, and this artificial milkiness shape is still very rare behind the autoclaving, need to add the milk powder of sterilization to improve its viscosity again.Be difficult for very fast dissolving but add milk powder, and produce little particulate material, could form liquid relatively uniformly after need grinding with mill;
3. filling a prescription 4 is prescription 3 improvement, and its maximum characteristics are to have increased rat blood serum.The breast of finding this formulated in the use solidifies behind autoclave sterilization, need grind with tissue grinder and could form liquid uniformly for a long time, but still have some little particles, and influence is digested and assimilated.And also bigger with the loss of the artificial milk of mill grinding, also to add entry during grinding, the water of adding is difficult quantitatively.In addition, find to feed suckling mouse with this milk in our test, can form hard big ziega as stone in the stomach of some suckling mouses, ziega accounts for more than 50% of gastric capacity, such ziega obviously influences suckling mouse to the sucking and emptying of breast, influences growing of suckling mouse then.
Summary of the invention
According to the pluses and minuses of above prescription, through a large amount of tests, develop a kind of new artificial feeding milk for sterilized rat, this artificial breast has changed the content of rabbit breast and serum, and has increased cow's milk and several aminoacid ingredient, artificial feeding sterilized rat survival rate height.
A kind of artificial feeding milk for sterilized rat is divided into 0-10 days groups and 11-21 days groups according to suckling mouse birth age in days, and in 100ml, its raw material composition is respectively:
Form suckling mouse birth age in days suckling mouse birth age in days
0-10 days group 11-21 days groups
Rabbit breast (ml) 51-59 59-75
5%-25% milk (ml) 25-44 10-25
Rat blood serum (ml) 5-15 15-18
Lactose (g) 11
Methionine (mg) 60-90 50-60
Lysine (mg) 60-90 50-60
Tryptophan (mg) 60-90 50-60
Vitamin A (u) 2600-15000 15000-25000
Vitamin D (u) 600-7000 7000-15000
Vitamin E (mg) 2.5-8 8-15
Vitamin B1 (mg) 2.5-9.5 9.5-15
Vitamin B2 (mg) 2.5-9.5 9.5-15
Vitamin B6 (mg) 1.5-6.0 6.0-15
Cobalamin (mg) 1-1.5 1.5-10
Vitamin C (mg) 25-50 50-100
Milk powder (g) 0 0-10
Pure water surplus surplus
The every 100ml of above-mentioned raw materials adds in addition:
Egg (g) 0 0-100
Protein powder (g) 0.1-1 1-15
Use the artificial breast of the present invention's preparation, rabbit breast and rat blood serum content have been reduced, added the amount of milk, and added three kinds of limiting amino acids, be characterized in, the formula components less complex, comprehensive nutrition does not solidify behind the autoclave sterilization, is formed with certain viscosity, uniform liquid, do not form tangible particle, only need with using after the filtered through gauze; Newborn rat is sucked easily, and gastric emptying is very fast, does not form ziega, and it is good to grow.This breast both can be used for gavage, also can be used for nature and sucked method.
The present invention simultaneously is according to the stronger prescription of the different specific aims that design of different days suckling mouse emptying ability and desired nutritional.Caesarean birth is the suckling mouse of birth just, has the born ability of sucking, but sucks strength and gastric emptying is all very low, and is to feed breast with made nipple, and suckling mouse also has a process of learning the adaptation that the artificial nipple of use sucks.Therefore, will guarantee the nutrient content of artificial breast, and keep it to have most suitable viscosity again, and be convenient to it and suck.If when viscosity surpassed its sucking ability, suckling mouse sucked the interior milk of stomach seldom, on the contrary air is sucked in the stomach.Stomach is very slow to the emptying of air, and just the sucking of influence milk seriously hinders suckling mouse to grow.Growth along with suckling mouse, its nutritional labeling demand is also in continuous increase, and suckling mouse has also adapted to artificial nipple gradually, can suck more freely, the strength of sucking is also increasing, if still use the prescription of giving newborn rat, nutritional labeling is not enough to some extent, and it is also fast that suckling mouse sucks, and so just might cause the breast of choking.So it is higher to make nutritional labeling into, and the bigger slightly breast of viscosity is fed.Suckling mouse constantly grows up, and composition of milk is also along with changing.5 days feed breast, the weight average of suckling mouse every day increases by 0.3 gram, 6-10 days, on average increases by 0.5 gram, 11-15 days average 1 grams that increase, 16-21 days average 1.5 grams that increase.
11-21 days groups of suckling mouse of the present invention birth age in days are breast manually, and especially suckling mouse behind 16 ages in days grows and the needs that strengthen own feed ability have designed the artificial breast that adds high nutrients such as protein powder and egg in order to satisfy.So big suckling mouse opens eyes, and tooth is also looked longer.Behind the artificial newborn autoclaving, can become the material that solidifies shape, be convenient to suckling mouse and sting food and chew, and, be beneficial to its growth behind breast, continuing to provide this food.Prepare so artificial breast and fully satisfy the needs in this age in days stage, the suckling mouse well-grown.
China does not have germfree animal now, if be about 100,000 yuan from the price of every pair of sterilized rat of external introduction, and in transportation easy contaminating microorganisms.Feed sterilized rat by the present invention, the survival rate height will be filled up the blank of domestic this respect, and bringing a high price of every rat is several times of common rat, and economic benefit is considerable.We use egg and protein powder can reduce the cost of this milk greatly.80 yuan every of conceived rabbits can only be gathered the rabbit milk about 20ml every day, but also will be managed with delicacy.If young rabbit death, female rabbit is just wean also.The cost of female rabbit feed every day about 5 yuan.The cost of rat blood serum is also very high, and the most cheap 18 yuan of every rat is gathered 5ml serum at most.Agalactous suckling mouse in 15 days, the 3-4g that need suckle every every day, so consumption is very big.The food that we also once nibbled food with rabbit breast and the rat blood serum preparation suckling mouse suckling mouse of feeding, finding does not have notable difference with food with egg and protein powder making aspect the body weight gain.Therefore we just greatly reduce the consumption of rabbit breast and rat, have saved cost like this.
Description of drawings
Fig. 1 is the suckling mouse of 16 ages in days, the sterilized rat growing state when not adding egg and protein powder and increase egg and protein powder.
The specific embodiment
Embodiment 1-5:
Prepare artificial feeding milk for sterilized rat by concrete raw material composition in table 2 and the table 3, be divided into 11-21 days groups according to suckling mouse birth age in days and feed sterilized rat:
Table 2 0-10 days group
Example 1, example 2, example 3 are that routine is used for the used prescription of suckling mouse birth age in days 1-10 days groups artificial suckling, and the suckling mouse of example 1 in particular for just being born in most of the cases can be used.But because the caesarean birth suckling mouse has bigger difference, sometimes Chu Sheng the suckling mouse vigor and the power of sucking are poor slightly, body weight is lower, breast sucking and emptying for preparation in the example 1 are relatively poor, just consider to make the one-tenth assignment system of use-case 4, the content of example 4 ratios 1 its rabbit breast, serum, protein powder and cow's milk concentration all descend to some extent, and viscosity also decreases.And when the ratio of suckling mouse growth generally speaking will be good, just can consider to have additional nutrients, to satisfy the needs that suckling mouse grows, resemble the prescription that provides in the example 5.
Table 3 11-21 days group
Example 1 in above-mentioned group, example 2 and example 3 are that conventional preparation is used for the artificial suckling of 11-21 days groups of suckling mouse birth age in days and sucks the artificial breast of usefulness, and example 4 is that suckling mouse stings the used prescription of food, in most of the cases can use.But, sometimes suckling mouse is nibbled power when relatively poor, can make the formulated in the use-case 5 when edible and digestion is not good for the egg cream sample material in the example 4.Example 5 has reduced the consumption of protein powder, egg and milk powder, and its hardness decreases, and than Evacuable and digestion, can be used for the edible of this class suckling mouse.
The present invention's prescription is suitable for sucking method naturally newborn caesarean birth rat being carried out artificial suckling, through repetition test, suck the different of power, emptying situation and nourishment according to suckling mouse, the prescription of suckling mouse is divided into suitable 0-10 ages in days and is fit to two parts of 11-21 age in days suckling mouses, and the growth that meets suckling mouse so more is required.
In prescription, increased from original egg and protein powder.Not adding egg in 0-10 days suckling mouse, mainly is in order to keep certain viscosity, because add a small amount of egg, viscosity will increase, thereby influences the sucking of suckling mouse.Adding protein powder is the protein content that can improve in the prescription, and does not improve the viscosity of artificial breast.For the later suckling mouse of 11 ages in days, sucking power and emptying digestion power improve constantly, and can increase the use amount of egg and protein powder, can also make the swollen soft feed of cake sample with other compositions with these two kinds of compositions, supply it to sting and eat and chew, can significantly improve the body weight of suckling mouse.
Methionine, lysine and tryptophan are so-called limiting amino acids, add such amino acid in right amount, except itself can directly be absorbed, can also promote other amino acid whose absorptions.Along with the increase of suckling mouse age in days, the suckling mouse digestion and absorption function constantly strengthens, and suitably reduces the use amount of this three seed amino acid.
We also are the vitamin that adds different amounts according to the difference of age in days for micronutrients such as vitamins, and along with the increase consumption of age in days also constantly increases.
In experiment, our use of vulture Jian Shi, HoshibaShi and RT-89 is all successfully fed and is lived through suckling mouse, almost all dies from and suffocates.
We use RT-91 to feed altogether and live 8, and we have fed 146 altogether, so survival rate is also very low, the prescription survival rate of using the embodiment of the invention 1-3 is higher than the nursing situation of the survival rate about 5% of using RT-91 far away about 60%.
The suckling mouse that utilizes formula feeding 16 ages in days of the present invention that Fig. 1 shows, the growing state of suckling mouse after protein powder and egg have been added in artificial Ruzhong (by 4 preparations of 11-21 age in days assembly sides).Prescription 1 is not for adding protein powder and egg (milk powder with equivalent replaces this two kinds of compositions), and prescription 2 adds these two kinds of compositions.
*p<0.05，
**p<0.01。What body weight increased behind interpolation protein powder and the egg is faster, has had significant difference during to 20 and 21 ages in days.As seen increased the consumption of egg and protein powder, feeding effect is obvious.
Claims (2)
1. artificial feeding milk for sterilized rat, suckling mouse birth age in days was suitable for for 0-10 days, and described artificial feeding milk for sterilized rat is in cumulative volume 100ml, and its raw material consists of:
Rabbit breast 51-59ml
5%-25% milk 25-44ml
Rat blood serum 5-15ml
Lactose 1g
Methionine 60-90mg
Lysine 60-90mg
Tryptophan 60-90mg
Vitamin A 2600-15000u
Vitamin D 600-7000u
Vitamin E 2.5-8mg
Vitamin B1 2.5-9.5mg
Vitamin B2 2.5-9.5mg
Vitamin B6 1.5-6.0mg
Cobalamin 1-1.5mg
Vitamin C 25-50mg
Milk powder 0g
The pure water surplus
The every 100ml of above-mentioned raw materials adds in addition:
Egg 0g
Protein powder 0.1-1g.
2. artificial feeding milk for sterilized rat, suckling mouse birth age in days was suitable for for 11-21 days, and described artificial feeding milk for sterilized rat is in cumulative volume 100ml, and its raw material consists of:
Rabbit breast 59-75ml
5%-25% milk 10-25ml
Rat blood serum 15-18ml
Lactose 1g
Methionine 50-60mg
Lysine 50-60mg
Tryptophan 50-60mg
Vitamin A 15000-25000u
Vitamin D 7000-15000u
Vitamin E 8-15mg
Vitamin B1 9.5-15mg
Vitamin B2 9.5-15mg
Vitamin B6 6.0-15mg
Cobalamin 1.5-10mg
Vitamin C 50-100mg
Milk powder 0-10g
The pure water surplus
The every 100ml of above-mentioned raw materials adds in addition:
Egg 0-100g
Protein powder 1-15g.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100604623A CN100462013C (en) | 2005-08-19 | 2005-08-19 | Artificial feeding milk for sterilized rat |
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| CNB2005100604623A CN100462013C (en) | 2005-08-19 | 2005-08-19 | Artificial feeding milk for sterilized rat |
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| CN1742608A CN1742608A (en) | 2006-03-08 |
| CN100462013C true CN100462013C (en) | 2009-02-18 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293334B (en) * | 2011-06-27 | 2013-04-24 | 浙江大学 | Improved sterile artificial rat feeding milk |
| CN114868843B (en) * | 2022-06-28 | 2024-05-07 | 睿迪生物科技（深圳）有限公司 | Special artificial milk for sterile young mice and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1507879A (en) * | 2002-12-15 | 2004-06-30 | 东北农业大学 | Method for preparing lacto coccus dry-powder product |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1507879A (en) * | 2002-12-15 | 2004-06-30 | 东北农业大学 | Method for preparing lacto coccus dry-powder product |
Non-Patent Citations (1)
| Title |
|---|
| 无菌大鼠的开发与应用研究.. 王荫槐等.中国实验动物学杂志.，第5卷第2期. 1995 * |
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