CN100427612C - Microwave induced method for catalytic preparation of starch hydrolyzed sugar - Google Patents
Microwave induced method for catalytic preparation of starch hydrolyzed sugar Download PDFInfo
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- CN100427612C CN100427612C CNB2006101369555A CN200610136955A CN100427612C CN 100427612 C CN100427612 C CN 100427612C CN B2006101369555 A CNB2006101369555 A CN B2006101369555A CN 200610136955 A CN200610136955 A CN 200610136955A CN 100427612 C CN100427612 C CN 100427612C
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Abstract
The invention discloses a making method of hydrolytic sugar of starch catalyzed and induced by microwave, which comprises the following steps: adopting rough starch as raw material; grinding to 100-150 order; adding water to allocate 15-40% starch liquid; adding 0.05-0.15% CaCl2; stirring evenly; allocating starch emulsion; adding 80-150U/g alpha-amylase and 80-100U/g glucose amylase; stirring evenly; irradiating through microwave; controlling the irradiating temperature at 50-80 deg. c; maintaining the inducing and catalytic time for 1-30min; heating the hydrolytic liquid over 90-150 deg. c for 0.5-1 min; killing the activity of alpha-amylase and glucose amylase; obtaining the product.
Description
Technical field
The present invention relates to a kind of preparation method of amylum hydrolysate of the sugar.
Background technology
The traditional method of starch hydrolysis has acid hydrolysis, enzymic hydrolysis, phytase hydrolysis, and wherein acid hydrolysis and enzymic hydrolysis are the industrial methods that generally adopts.Acid hydrolysis is because the pH value that requires is very low, and carries out under the condition of High Temperature High Pressure, so to the having relatively high expectations of corrosion-resistant, the high-temperature-resistant high-pressure-resistant of equipment, the while hydrolysis time is very long, starch concentration is at 16-25%, and products obtained therefrom is of poor quality; Enzymic hydrolysis is the method for more use in the existing production, but owing to adopt required chronic (24-48 hour) of enzymic hydrolysis, starch concentration is at 16-25%, and also higher 90-145 ℃ of the temperature of requirement so required energy consumption is higher, also has a strong impact on the production cycle." chemistry world " (2000,41 (7)) disclose " research of microwave irradiation technology in starch hydrolysis system glucose response ", adopt hydrochloric acid to make catalyzer, hydrolyzed starch under the microwave radiation condition.The shortcoming that this method exists is to adopt hydrochloric acid to make catalyzer, the corrosion-resistant of equipment had relatively high expectations, hydrochloric acid is volatile in addition, health and production environment to the operator are influential, starch concentration lower (16-25%), products obtained therefrom of poor quality, the acidity of system was higher after hydrolysis finished, need to adopt the alkali neutralization, the salt ion that exists in the system is very big to the influence of subsequent production, as the refining and microbial fermentation of glucose.
Summary of the invention
The purpose of this invention is to provide the preparation method that a kind of microwave induction catalytic prepares amylum hydrolysate of the sugar.
The objective of the invention is to realize in the following way: a kind of microwave induction catalytic prepares the method for amylum hydrolysate of the sugar, its preparation process is: with the starch coarse raw materials, be crushed to the 100-150 order, add the starch water liquid that water is made into 15%-40%, add the CaCl of 0.05-0.15% simultaneously
2Stir, be made into starch milk, in starch milk, add the α-Dian Fenmei and the 80-100U/g starch metering glucoamylase of 80-150U/g starch metering, after stirring, carry out microwave radiation, the microwave radiation temperature is controlled at 50-80 ℃, microwave induction catalytic hydrolysis time 1-30 minute, in microwave field, be warming up to the hydrolyzed solution that obtains more than 90-150 ℃ rapidly, kept 0.5-1 minute, the α-Dian Fenmei of going out and glucose-amylase activity get amylum hydrolysate of the sugar.
Present method also can:
1) carry out the microwave induction catalytic hydrolysis earlier: add the α-Dian Fenmei of 80-150U/g starch metering in starch milk, after stirring, carry out microwave radiation, the microwave radiation temperature is controlled between 50-80 ℃, microwave induction catalytic hydrolysis time 1-30 minute; The enzyme that goes out is then handled: the liquefier that obtains is warming up to 145-150 ℃ rapidly in microwave field, keeps 30-60 second, alpha-amylase activity goes out;
2), carry out the microwave induction catalytic saccharification again: in hydrolyzed solution, add 80-100U/g starch metering glucoamylase, after stirring, place in the microwave radiation field, the microwave radiation temperature is controlled between 50-80 ℃, microwave induction catalytic saccharification time 1-30 minute; The enzyme that goes out is then handled: saccharification liquid is warming up to 90-100 ℃ rapidly in microwave field, keeps 1 minute, and the glucose-amylase activity that goes out gets amylum hydrolysate of the sugar.
Microwave induction catalytic hydrolysis time preferably 2-4 minute.
Regulate the pH value of hydrolyzed solution when carrying out the microwave induction catalytic saccharification to 4.0-5.0.
The present invention has following beneficial effect: 1, can hydrolysis under the starch milk condition of higher concentration, and starch concentration can reach 30%-40%; 2, compare with classical acid hydrolysis, enzymic hydrolysis, hydrolysis time can shorten greatly; 3, this method to equipment acidproof, requirement of withstand voltage is lower, production technique belongs to friendly process; 4, products therefrom reducing sugar content height, the product transparency is good, and the percent hydrolysis height is particularly suitable for follow-up fermentation and makes raw material usefulness.
Embodiment
Embodiment 1
Integrated microwave induction catalytic prepares the process of amylum hydrolysate of the sugar:
1, the preparation of starch milk: get starch coarse raw materials 100 gram, be crushed to 150 orders, in the microwave special utensil, add water and be made into 30% starch water liquid, add the CaCl about 0.15% simultaneously
2, stir, be made into starch milk;
2, microwave induction catalytic: in the vessel that deployed starch milk is housed, add α-Dian Fenmei 15000U, add glucoamylase 10000U, after stirring, place in the microwave oven, microwave irradiation power 800W, the microwave radiation temperature is controlled at about 65 ℃, microwave induction catalytic hydrolysis time 2-4 minute;
3, the enzyme that goes out is handled: the hydrolyzed solution that obtains is warming up to rapidly in microwave field more than 100 ℃, and maintenance 1min, the α-Dian Fenmei of going out and glucose-amylase activity, the amylum hydrolysate of the sugar that makes is as the raw material of glucose industry, fermentation industry and foodstuffs industry.
Embodiment 2
Microwave induction catalytic prepares the process of amylum hydrolysate of the sugar stage by stage:
1, the preparation of starch milk: get starch coarse raw materials 100 gram, be crushed to 150 orders, in the microwave special utensil, add water and be made into 30% starch water liquid, add the CaCl about 0.15% simultaneously
2, stir, be made into starch milk;
2, microwave induction catalytic liquefaction: in the vessel that deployed starch milk is housed, add α-Dian Fenmei 15000U, after stirring, put into microwave oven, microwave irradiation power 800W, the microwave radiation temperature is controlled between 60-80 ℃, microwave induction catalytic hydrolysis time 2-4 minute; The enzyme that goes out is then handled, and the liquefier that obtains is warming up to 145-150 ℃ rapidly in microwave field, keeps 1 minute, and alpha-amylase activity goes out;
3, microwave induction catalytic saccharification: the pH value of regulating liquefier is added glucoamylase 10000U, after stirring to 4.0-5.0, place in the microwave oven, microwave irradiation power 800W, the microwave radiation temperature is controlled between 50-60 ℃, microwave induction catalytic saccharification time 2-4 minute; The enzyme that goes out is then handled, and saccharification liquid is warming up to 90 ℃ rapidly, keeps 1 minute, and glucose-amylase activity goes out.Make amylum hydrolysate of the sugar, by analysis, wherein dextrose equivalent value (DE value) reaches 100%.
Claims (4)
1, a kind of microwave induction catalytic prepares the method for amylum hydrolysate of the sugar, and its preparation process is: with the starch coarse raw materials, be crushed to the 100-150 order, add the starch water liquid that water is made into 15%-40%, add the CaCl of 0.05-0.15% simultaneously
2Stir, be made into starch milk, in starch milk, add the α-Dian Fenmei and the 80-100U/g starch metering glucoamylase of 80-150U/g starch metering, after stirring, carry out microwave radiation, the microwave radiation temperature is controlled at 50-80 ℃, microwave induction catalytic hydrolysis time 1-30 minute, the hydrolyzed solution that obtains is warming up to 90-150 ℃ rapidly in microwave field, kept 0.5-1 minute, the α-Dian Fenmei of going out and glucose-amylase activity get amylum hydrolysate of the sugar.
2, a kind of microwave induction catalytic according to claim 1 prepares the method for amylum hydrolysate of the sugar, it is characterized in that:
1) carry out the microwave induction catalytic hydrolysis earlier: add the α-Dian Fenmei of 80-150U/g starch metering in starch milk, after stirring, carry out microwave radiation, the microwave radiation temperature is controlled between 50-80 ℃, microwave induction catalytic hydrolysis time 1-30 minute; The enzyme that goes out is then handled: the liquefier that obtains is warming up to 145-150 ℃ rapidly in microwave field, keeps 30-60 second, alpha-amylase activity goes out;
2), carry out the microwave induction catalytic saccharification again: in hydrolyzed solution, add 80-100U/g starch metering glucoamylase, after stirring, place in the microwave radiation field, the microwave radiation temperature is controlled between 50-80 ℃, microwave induction catalytic saccharification time 1-30 minute; The enzyme that goes out is then handled: saccharification liquid is warming up to 90-100 ℃ rapidly in microwave field, keeps 1 minute, and the glucose-amylase activity that goes out gets amylum hydrolysate of the sugar.
3, a kind of microwave induction catalytic according to claim 2 prepares the method for amylum hydrolysate of the sugar, it is characterized in that: the microwave induction catalytic hydrolysis time is 2-4 minute.
4, a kind of microwave induction catalytic according to claim 2 prepares the method for amylum hydrolysate of the sugar, it is characterized in that: regulate the pH value of hydrolyzed solution when carrying out the microwave induction catalytic saccharification to 4.0-5.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101369555A CN100427612C (en) | 2006-12-27 | 2006-12-27 | Microwave induced method for catalytic preparation of starch hydrolyzed sugar |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101369555A CN100427612C (en) | 2006-12-27 | 2006-12-27 | Microwave induced method for catalytic preparation of starch hydrolyzed sugar |
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| CN1995398A CN1995398A (en) | 2007-07-11 |
| CN100427612C true CN100427612C (en) | 2008-10-22 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110333329A (en) * | 2019-05-08 | 2019-10-15 | 重庆瑞钛科技有限公司 | The rapid detection method of amylopectin content in wine brewing grain |
| CN110724718A (en) * | 2019-12-02 | 2020-01-24 | 保龄宝生物股份有限公司 | Method for preparing resistant dextrin by using microwaves |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0568218B2 (en) * | 1988-03-04 | 1993-09-28 | Junichi Azuma | |
| CN1412324A (en) * | 2001-10-18 | 2003-04-23 | 山东中舜科技发展有限公司 | Technological improvement on glucose production |
| CN1880486A (en) * | 2005-06-13 | 2006-12-20 | 山东西王糖业有限公司 | Process for improving production capacity of glucose |
-
2006
- 2006-12-27 CN CNB2006101369555A patent/CN100427612C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0568218B2 (en) * | 1988-03-04 | 1993-09-28 | Junichi Azuma | |
| CN1412324A (en) * | 2001-10-18 | 2003-04-23 | 山东中舜科技发展有限公司 | Technological improvement on glucose production |
| CN1880486A (en) * | 2005-06-13 | 2006-12-20 | 山东西王糖业有限公司 | Process for improving production capacity of glucose |
Non-Patent Citations (2)
| Title |
|---|
| 微波加热淀粉糖化. 周秀琴.发酵科技通讯，第1996卷第4期. 1996 * |
| 微波辐射技术在淀粉水解制葡萄糖反应中的研究. 夏立新，李坤兰，曹国英，奚祖威.化学世界，第2000卷第7期. 2000 * |
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