CN100410652C - Teaching kit for cell-level organism evolution - Google Patents
Teaching kit for cell-level organism evolution Download PDFInfo
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- CN100410652C CN100410652C CNB2006100232451A CN200610023245A CN100410652C CN 100410652 C CN100410652 C CN 100410652C CN B2006100232451 A CNB2006100232451 A CN B2006100232451A CN 200610023245 A CN200610023245 A CN 200610023245A CN 100410652 C CN100410652 C CN 100410652C
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Abstract
The present invention relates to a teaching kit for cell-level organism evolution. Aiming at an experiment of cell-level organism evolution, reagents and material carry out optimizing configuration according to ten groups forming one experiment teaching standard class. The present invention comprises a scalpel, toothpicks, cover glass, a pair of forceps, glass slide, a culture tubule (a glycerol pipe is used for storage after stab culture of E. coli), 0.02% of Jenner's green liquor, a small culture plate of which the diameter is 6cm, a distilled water bottle of which the volume is 10 ml., a cultured base (dry powder), 50 ml. of distilled water, etc. A service manual is prepared in each kit which can be used for a standard class to experiment. The present invention can be applied to bioscience experiment introduction teaching, and can save the experiment preparation time for teachers and reduce the configuration of experimenters and the technology difficulty of experiment preparation. The cell-level organism evolution experiment can be quickly completed in high efficiency, and favorable experiment effect can be obtained.
Description
Technical field
The present invention relates to a kind of teaching kit of biological evolution of cellular level, be used for the biological evolution experiment of biological scientific experiment introduction teaching cellular level.
Background technology
At present, China's biological scientific experiment teaching faces to have high input, produce imitates low awkward situation, and general laboratory all needs to dispose simultaneously experiment teacher and laboratory technician.Before experiment, need to do a large amount of preliminary work, often will buy earlier and test relevant plurality of reagents and material, the preparation of dividing into groups of the number situation of experimental then requirement, experiment.Experimenter's configuration that the finishing of these work needs some, reagent of Gou Maiing and material be often all greater than the consumption of actual needs simultaneously, thereby existence waste in various degree, improved experimental cost.The technical difficulty of testing preliminary work simultaneously is also very high, and to the use amount of each experiment group, to the accurate tolerance and the distribution of every kind of reagent, every kind of material usage, it is careful accurate all to require, to guarantee normally carrying out of experiment.This shows that preparing experiment need consume and waste great amount of manpower and material resources, financial resources, be badly in need of a kind of mode of new preparing experiment.Though it is very general that domestic and imported scientific research kit is used in China's bio-science research, have convenient, fast, efficient and result's characteristics accurately and reliably, but do not see the kit patented product that is applicable to the teaching of biological scientific experiment introduction so far at home as yet.Because the scientific research kit generally all at a certain particular experiment link, has very strong technical characterictic, its content is difficult to the education experiment on the adaptive surface simultaneously.Thereby, invent a kind of teaching kit of biological scientific experiment teaching that is applicable to and seem particularly necessary and urgent.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art and actual needs, a kind of teaching kit that is applicable to the biological evolution of cellular level is provided, can directly be used for finishing education experiment easily, guarantee the high-level efficiency of experiment, save experimental cost.
For realizing such purpose, the present invention is directed to the biological evolution experiment of cellular level, reagent and material are contained in the kit, and are optimized configuration by 10 groups one experimental teaching standard class.Material in the kit is according to the use amount design of 10 experiment groups, comprise bacterial classification tubule (E coli, Escherichia coli punctures cultivate back glycerine guarantee deposit), tweezers, scalper, the green dye liquor of 0.02% Zhan Na Shi, distilled water drop bottle, little double dish, microslide, cover glass, toothpick, LB nutrient culture media (Luria-Bertani Medium dry powder shape contains agar powder), distilled water etc.Be equipped with service manual in each kit, can carry out experiment for a standard class.
Teaching kit of the present invention contains a complete set of material and the reagent of the biological evolution experiment of cellular level.Lay material by 10 experiment group use amounts in the kit, every group comprises that a scalper, one bag of toothpick, clad slide, wrap microslide, one bottle of green dye liquor of Zhan Na Shi, a little double dish, one bottle of distilled water drop bottle etc. to tweezers.The consumption of every group reagent and material is that wherein the green dye liquor weight percent concentration of Zhan Na Shi is 0.02% through the experimental teaching optimal value groping to obtain repeatedly over the years, and the distilled water drop bottle is 10ml, and double dish is 6cm, 3 of microslides, 10 of cover glasses, 2 on toothpick.Be equipped with in addition a bacterial classification tubule in the kit, the coli strain that built-in glycerine is preserved, one bottle of LB nutrient culture media (dry powder, 0.625g), one bottle of 50ml distilled water and a kit service manual.
One group of material can use for one group of student (2-3 people) in the kit of the present invention, and ten groups of materials can be carried out experiment for a standard class (30 people).Can also be equipped with corresponding experiment instruction teaching material and CD according to different needs.
Before the experiment, experiment teacher or preparation personnel only need do simple preliminary work: the teacher is before giving a course in experiment, adds 50ml distilled water in culture medium dry powder, preparation LB nutrient culture media, with high-temperature sterilizations such as little double dish of 6cm and toothpicks, on superclean bench, fall dull and stereotyped 10; 24 hours in advance line activation Escherichia coli on tablet; The E.coli that activation is good can be placed on 4 ℃ of refrigerators and preserve, and is distributed to every group before the experiment.Fresh dayflower can be gathered in experiment the same day.To test distribution of goods to every group before the experiment beginning.During experiment, carrying out the green dyeing of E coli thalline Zhan Na Shi earlier observes: central authorities drip the one 0.02% green dyeing liquor of Zhan Na Shi at slide, Escherichia coli bacterium colony with the fresh growth of transfer needle picking, in dye liquor, be coated with and launch, make it to be evenly distributed, with dyeing 3min, add cover glass, examine under a microscope.Bacterial cell is dyed green, and is shaft-like, and the bacterium that lives can observe its motion conditions at microscopically.The green dyeing of human oral mucosa's cell mitochondrial Zhan Na Shi is observed: gets the green dye liquor of 0.02% Zhan Na Shi and places clean microslide central authorities, scrape with the sterilization toothpick and get the oral cavity inwall, and firmly heavy slightly, to obtain the more vigorous cell of vitality.Get thing and place dye liquor on the microslide scraping, mixing, after 2～3 minutes, covered places microscopically to observe.High power lens is observed behind elder generation's low power lens, and in the tenuigenin of visible oral mucosa cell, some are dyed the shaft-like and circular granular of bright green to be dispersed in distribution, are mitochondria under high power lens.Observe mitochondrial form and dyeing situation in the human body oral mucosa cell, relatively the something in common of itself and Escherichia coli form and dyeing.The observation of dayflower epidermal cell: get clean microslide earlier, add in the above and drip clear water.Get fresh duck sole of the foot blade of grass sheet, with blade at breach of vacuum side of blade crosscut, tear with surperficial parallel insertion tweezers from the breach place and to get epidermis, face down after epidermis is torn and put into the water droplet of microslide immediately, covered, inhale with thieving paper and to remove excessive moisture, can observe structures such as form at the microscopically vegetable cell, size, chloroplast, vacuole.
The coli strain that teaching kit of the present invention provides a pipe glycerine to preserve, the experiment teacher needs only its activation, and distribution of goods can be carried out experiment to each group.Reduced experimenter's configuration so on the one hand, also made experimental teaching quick, efficient on the other hand, the student can obtain standard of comparison, reliable experimental result.
The present invention is optimized configuration with experiment reagent and material by 10 groups one experimental teaching standard class for the teaching of biological scientific experiment introduction provides a kind of economical and practical teaching kit, has avoided unnecessary waste, has saved reagent and material.During experiment, after the teacher reads the kit service manual, need only according to explanation every group of required material and reagent are distributed to each group in conjunction with explaining the teaching that can experimentize, saved loaded down with trivial details experiment and prepared the time and efforts of having saved the teacher.Simultaneously, the raising of work efficiency makes experimenter's configuration that certain minimizing can be arranged.The service manual that the student has according to teacher's classroom explanation and kit can have good grasp and finish experiment smoothly experiment content in the short period of time.Kit of the present invention makes the biological evolution experiment of cellular level can finish and obtain good experiment effect efficiently, fast, and financial resources, material resources, the manpower that experiment can be prepared required consumption drop to minimum.
Description of drawings
Fig. 1 is the reagent that kit of the present invention comprised and the displaying of medicine.
Among the figure, 1 is kit, and 2 is scalper, 3 is toothpick, and 4 is cover glass, and 5 is tweezers, 6 is microslide, 7 are bacterial classification tubule (E coli puncture cultivate back glycerine guarantee deposit), the 8 green dye liquors of Zhan Na Shi that are weight percentage concentration 0.02%, and 9 is the little double dish of 6cm, 10 is 10ml distilled water drop bottle, 11 is LB nutrient culture media (dry powder), and 12 is 50ml distilled water, and 13 is the kit service manual.
Embodiment
Below in conjunction with accompanying drawing technical scheme of the present invention is further described.
Fig. 1 is an embodiment of teaching kit provided by the invention, has comprised the required a complete set of material and the reagent of biological evolution experiment of cellular level in the kit.As shown in Figure 1, experiment reagent and material are optimized configuration by 10 groups one experimental teaching standard class, comprise 10 materials that group is required in the kit 1, every group comprises a scalper 2, one bag of toothpick 3,10 cover glasses 4 of one bag, tweezers 5,3 microslides 6 of one bag, one bottle of weight percent concentration is 0.02% the green dye liquor 8 of Zhan Na Shi, a 6cm double dish 9, one bottle of 10ml distilled water drop bottle 10, be equipped with common material in the kit in addition and comprise a bacterial classification tubule 7, the coli strain that built-in glycerine is preserved, one bottle of 0.625g LB nutrient culture media 11 (dry powder), one bottle of 50ml distilled water 12 and a kit service manual 13.
In the kit in above-mentioned each inner wrapping the consumption of material be the optimal value that experimental teaching over the years obtains, one group of material uses for one group of student (2-3 people), ten groups of materials can be carried out experiment for a standard class (30 people).
During experiment, each experiment group is assigned in every group of material taking-up in the kit, according to the explanation of service manual, the teacher explains and can finish experiment by instruction of papil.In addition, can also dispose corresponding experiments supporting in the kit according to different needs and instruct teaching material and CD, combining with teaching experimentizes, better effects if.
Claims (2)
1. the teaching kit of the biological evolution of a cellular level, it is characterized in that the required reagent and the material of biological evolution experiment of cellular level is optimized configuration by 10 groups one experimental teaching standard class, every group comprises that a scalper (2), one bag of toothpick (3), bag 10 cover glasses (4), one wrap 3 microslides (6), one bottle of green dye liquor of Zhan Na Shi (8), 6 cm double dish (9), one bottle of 10ml distilled water drop bottle (10) to tweezers (5),, and wherein the green dye liquor weight percent concentration of Zhan Na Shi is 0.02%; Be equipped with a bacterial classification tubule (7), one bottle of 0.625g LB culture medium dry powder (11), one bottle of 50ml distilled water (12) and a kit service manual (13) in the kit in addition, wherein the coli strain of the built-in glycerine preservation of bacterial classification tubule (7).
2. according to the teaching kit of the biological evolution of the cellular level of claim 1, it is characterized in that also being equipped with experiment instruction teaching material and CD in the kit (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100232451A CN100410652C (en) | 2006-01-12 | 2006-01-12 | Teaching kit for cell-level organism evolution |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100232451A CN100410652C (en) | 2006-01-12 | 2006-01-12 | Teaching kit for cell-level organism evolution |
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| Publication Number | Publication Date |
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| CN1804599A CN1804599A (en) | 2006-07-19 |
| CN100410652C true CN100410652C (en) | 2008-08-13 |
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| CNB2006100232451A Expired - Fee Related CN100410652C (en) | 2006-01-12 | 2006-01-12 | Teaching kit for cell-level organism evolution |
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| CN113176217A (en) * | 2021-03-29 | 2021-07-27 | 西安理工大学 | Carrot-based chemical and material analysis professional course experimental method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5624638A (en) * | 1993-05-05 | 1997-04-29 | Davcotech, Inc. | Modular laboratory equipment and coupling system |
| CN1172529A (en) * | 1995-02-03 | 1998-02-04 | 竹崎悌二 | Method of fixedly supporting biopsy specimen, fixedly supporting agent, and embedding cassette |
| US20030207456A1 (en) * | 1998-09-18 | 2003-11-06 | Cytyc Corporation | Method and apparatus for preparing cytological specimens |
| WO2004059441A2 (en) * | 2002-12-20 | 2004-07-15 | Dakocytomation Denmark A/S | Information notification sample processing system and methods of biological slide processing |
| CN1556410A (en) * | 2003-12-31 | 2004-12-22 | 国家海洋局第一海洋研究所 | Detecting reagent box for fish virus and its detecting method |
| CN1181330C (en) * | 1999-04-23 | 2004-12-22 | 莱卡显微系统努斯洛赫股份有限公司 | Automatic dyeing device for dyeing microscopic examination object |
-
2006
- 2006-01-12 CN CNB2006100232451A patent/CN100410652C/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5624638A (en) * | 1993-05-05 | 1997-04-29 | Davcotech, Inc. | Modular laboratory equipment and coupling system |
| CN1172529A (en) * | 1995-02-03 | 1998-02-04 | 竹崎悌二 | Method of fixedly supporting biopsy specimen, fixedly supporting agent, and embedding cassette |
| US20030207456A1 (en) * | 1998-09-18 | 2003-11-06 | Cytyc Corporation | Method and apparatus for preparing cytological specimens |
| CN1181330C (en) * | 1999-04-23 | 2004-12-22 | 莱卡显微系统努斯洛赫股份有限公司 | Automatic dyeing device for dyeing microscopic examination object |
| WO2004059441A2 (en) * | 2002-12-20 | 2004-07-15 | Dakocytomation Denmark A/S | Information notification sample processing system and methods of biological slide processing |
| CN1556410A (en) * | 2003-12-31 | 2004-12-22 | 国家海洋局第一海洋研究所 | Detecting reagent box for fish virus and its detecting method |
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| CN1804599A (en) | 2006-07-19 |
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