CN100391346C - Effect enhanced sterilization composition and effervescence agent thereof - Google Patents
Effect enhanced sterilization composition and effervescence agent thereof Download PDFInfo
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- CN100391346C CN100391346C CNB2005101352371A CN200510135237A CN100391346C CN 100391346 C CN100391346 C CN 100391346C CN B2005101352371 A CNB2005101352371 A CN B2005101352371A CN 200510135237 A CN200510135237 A CN 200510135237A CN 100391346 C CN100391346 C CN 100391346C
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- bacillus subtilis
- jinggangmycin
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- propiconazole
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Abstract
The present invention relates to a synergism sterilization composition and an effervescence agent thereof. The synergism sterilization composition comprises bacillus subtitles with effective doses of effect enhancement, jinggangmycin, and propiconazole or thifluzamide. The composition of the present invention has obvious synergistic effect on the prevention and cure of rice sheath blight disease. The effervescence agent is used conveniently and has excellent prevention and cure effects.
Description
The application is to be on December 16th, 2003 applying date, and application number is 200310121389.7, and denomination of invention is dividing an application of " a kind of Synergistic bactericidal composition and effervescent agent thereof ".
Technical field
The present invention relates to a kind of new Synergistic bactericidal composition of preventing and treating rice sheath blight disease and effervescent agent thereof, more particularly, the present invention relates to a kind of bacillus subtilis that contains the synergy effective dose and jinggangmycin, propiconazole or thifluzamide.
Background technology
Paddy rice is one of human staple food crop, and the paddy rice plantation spreads all over a plurality of countries such as China, Japan, Thailand, and area reaches several hundred million mus.Banded sclerotial blight in the paddy rice planting process (Rhizoctoniasolani k ü hn) is the most common and topmost disease, almost all big generation in every year, and the field piece morbidity that the plantation level is high more is heavy more.Therefore, in paddy rice plantation main producing region, every paddy rice circle obstructs, forms ears season, and no matter disease takes place still not take place, and all will make a call to one time to two times and prevent and treat the agricultural chemicals of rice sheath blight disease.
Prevent and treat the used agricultural chemicals of rice sheath blight disease at present and mainly contain Polyoxin, jinggangmycin (validamycin), iprobenfos, propiconazole, thifluzamide and bacillus subtilis etc.But the control efficiency to rice sheath blight disease of these Pesticidal products still can not be satisfactory.In addition, the existing preparation formulation of these products mostly is water solube powder, wetting powder, aqua, water-soluble granular formulation and aqueous suspension etc.These preparations all need to convert water spray in use, and the peasant must carry heavy sprayer and soup and trek in the muddy water of rice field and spray.
Therefore, still just need a kind of better rice sheath blight disease controlling medicament and the convenient formulation of using.The present inventor finds with bacillus subtilis and jinggangmycin, propiconazole or thifluzamide combination, to have the Synergistic sterilizing effect, and these bactericidal compositions can be mixed with effervescent agent, so finished the present invention.
Summary of the invention
The purpose of this invention is to provide a kind of Synergistic bactericidal composition and effervescent agent thereof, it can be used to prevent and treat rice sheath blight disease.
[technical scheme]
Specifically, the invention provides a kind of bactericidal composition, it contains bacillus subtilis and jinggangmycin, propiconazole or the thifluzamide as active component of synergy effective dose.
Preferably, in bactericidal composition of the present invention, be 1～250: 250～1 as the bacillus subtilis of active component and the weight ratio of jinggangmycin, propiconazole or thifluzamide.More preferably, the weight ratio of bacillus subtilis and jinggangmycin is 1～180: 180～1, and the weight ratio of bacillus subtilis and propiconazole is 1～200: 200～1, and the weight ratio of bacillus subtilis and thifluzamide is 1～250: 250～1.
Among the present invention, bacillus subtilis can be its various bacterial strains, preferably the bacterial strain in BS-208 bacterial strain, Y3 bacterial strain, Y2-10 bacterial strain, 9-5-5 bacterial strain and " trying hard to keep " and " hundred is anti-" commodity preparation for example.They and jinggangmycin, propiconazole, thifluzamide etc. are mixed and all express obvious synergistic effect when preventing and treating rice sheath blight disease.
The BS-208 bacterial strain of bacillus subtilis, Y3 bacterial strain, Y2-10 bacterial strain, 9-5-5 bacterial strain all are known bacterial strains.Referring to, for example " bacillus subtilis BS-208 bactericide assessment of result material compilation " (.2003 of Wuhan Nature August)
Jinggangmycin, propiconazole or thifluzamide all are known agricultural chemicals.
Bactericidal composition of the present invention can be mixed with various formulations.In the present invention, bactericidal composition of the present invention preferably is mixed with effervescent agent.
A kind of formulation that contains alkali compounds (for example carbonate, bicarbonate etc.) and solid, water soluble acid compound (for example organic acid) combination of effervescent agent.In aqueous medium, alkaline matter and the reaction of solid, water soluble acidic materials produce carbon dioxide, make the effervescent agent disintegration, and the active component in the delivery formulations, reach the effect of prevention and elimination of disease and pests.Effervescent agent can be made for example particle or sheet form.
Therefore, the present invention also provides a kind of effervescent agent of above-mentioned bactericidal composition, and it forms with content as follows:
Active component 0.5-50% is preferably 10-40%;
Disintegrant acid compound 4%-25% is preferably 5-20%;
Alkali compounds 4%-25% is preferably 5-20%;
Adhesive 2%-30% is preferably 5-20%;
Surfactant 1%-20% is preferably 3-15%;
Help and collapse agent 1%-20%, be preferably 3-15%;
The filler surplus.
Can also contain 1～15% high bleeding agent in effervescent agent of the present invention.
The auxiliary agent that can be used for effervescent agent of the present invention requires not and the pesticide activity component generation chemical reaction of preventing and treating rice sheath blight disease, can not kill the bacterial spore in the preparation.Preferably require good penetrability.
Can be used for each analog assistant of the present invention is listed below:
Acid compound is selected from the following material of one or two or more kinds: citric acid, tartaric acid, malic acid, adipic acid, boric acid, citric acid potassium dihydrogen, potassium hydrogen tartrate, fumaric acid sodium and lactic acid;
Alkali compounds is selected from the following material of one or two or more kinds: sodium carbonate, sodium bicarbonate, potash, saleratus and calcium carbonate;
Adhesive is selected from the following material of one or two or more kinds: isopropyl alcohol or the ethanol liquid of polyethylene glycol, polyvinylpyrrolidone and polyvinyl acetate copolymer and PEG (12,000-20,000);
Surfactant is selected from the following material of one or two or more kinds: neopelex, the withered powder of tea, sodium lignosulfonate, Lignosite;
Help and collapse agent and be selected from the following material of one or two or more kinds: starch, sodium carboxymethylcellulose, carboxy-propyl cellulose and polyvinylpyrrolidone;
Filler is selected from the following material of one or two or more kinds: precipitated calcium carbonate, diatomite, kaolin, sericite in powder and white carbon black.
High bleeding agent is selected from a kind of following material below two kinds: azone, thiophene ketone and NNO.
Can also contain the adjuvant that other increases agricultural chemicals effect or processing characteristics, for example anticaking agent.
The pathogen Rhizoctonia solani of rice sheath blight disease survives the winter with the form of sclerotium.Spring sclerotium grow mycelia, infect rice plant from paddy rice base portion leaf sheath.
Before effervescent agent of the present invention is used; the paddy field is suitably more irritates some water, keeps depth of water 1cm～2cm, after effervescent agent particle or sheet are shed entry; the reaction of generation acid-base neutralization is bubbled and is seethed in water, as power the active ingredient in effervescent agent particle or the sheet is distributed in the water in monoblock paddy field.In addition, by the osmosis of high bleeding agent, the antibacterial substance in the water upwards soaks into and infiltration along the rice stem, reaches dried up the above position of 1cm, to paddy rice base portion leaf sheath, plays the effect that prevents that the pathogen mycelia from infecting.After dispenser finishes, make that water irrigating depth reduces to about 1cm in the paddy field.In order to make the preparation efficiency time longer; can will there be the gemma of some bacterial strain of obvious inhibiting bacillus subtilis (Bacillus subtilis) to be prepared into former powder to rice sheath blight disease; the mixing ratio that obtains by screening is incorporated in its combination pairing; make particle or compacting in the lump in flakes, throwing applies in the rice field.These gemma can be sprouted into trophosome and propagation, and the rice shoot base portion moisture film that forms by bleeding agent moves to than the high 1-2 of the water surface centimetre position to be grown surely and breed, plays the effect of prolongation preventive effect.
[beneficial effect]
The present composition has obvious synergistic effect to rice sheath blight disease controlling, and its effervescent agent is easy to use and have an excellent control efficiency.
Hereinafter the invention will be further described with non-limiting examples.
Embodiment
Embodiment 1
Bacillus subtilis BS-208 and jinggangmycin mixing effervescence granular (or effervescent tablet)
A. fill a prescription
Bacillus subtilis BS-208 bacterium powder (1000cfu/g) 20%
Jinggangmycin (200,000 units/g) 12%
Sodium bicarbonate 4.3%
Sodium carbonate 5.8%
Citric acid 8.5%
Fumaric acid 1.8%
Polyethylene glycol 15%
Neopelex 6.0%
Sodium carboxymethylcellulose 6.0%
Azone (40% powder) 6.0%
The diatomite surplus.
B. manufacture process
Calculate the blanking amount of every kind of material according to the above ratio, take by weighing out various materials respectively like clockwork.Start mixing machinery, earlier BS-208, jinggangmycin, diatomite, sodium carboxymethylcellulose, citric acid, fumaric acid are mixed, add azone and mixing again, add alkali compounds and other auxiliary agent and mixing at last.Pulverize one time through pulverizing mill, the material fineness is reached more than 200 orders.Comminutor (extruder grain method) the one-shot forming extruder grain (or compressing tablet) of packing into, about 2 millimeters of granule particle diameter, every heavy 1.3 grams of tablet.Dry good granule is packaged as finished product through after the assay was approved.
Embodiment 2
Bacillus subtilis preparation " hundred is anti-" and Jinggangmei mixing effervescent (or effervescent tablet)
A. fill a prescription
Bacillus subtilis formulation " hundred is anti-" (commercially available) 20%
Jinggangmycin (200,000 units/g) 14%
Sodium bicarbonate 5.0%
Sodium carbonate 5.5%
Citric acid 9.0%
Fumaric acid 1.2%
Polyethylene glycol 20%
Neopelex 6.0%
Sodium carboxymethylcellulose 5.0%
Azone (40% powder) 7.0%
The diatomite surplus.
B. manufacture process
Calculate the blanking amount of every kind of material according to the above ratio, accurately take by weighing various materials respectively.Start mixing machinery diatomite, " hundred anti-", jinggangmycin, carboxymethyl cellulose are mixed, add adhesive polyethylene glycol, azone, fumaric acid and citric acid again and mix, add alkali compounds and other various auxiliary agents mixing more at last.Pulverize one time through pulverizing mill, the material fineness is reached more than 200 orders.With Squeezinggranulator one-shot forming granulation or compressing tablet.Dry good granula (tablet) can be packed after the assay was approved and be dispatched from the factory.
Embodiment 3
Bacillus YZ-10 bacterium powder and jinggangmycin mixing effervescence granular (or effervescent tablet)
A. fill a prescription
The former powder (5 * 10 of bacillus subtilis YZ-10
10Cfu/g) 20%
Jinggangmycin (200,000 units/g) 12%
Sodium carbonate 9.2%
Citric acid 9.0%
Polyethylene glycol 18%
Neopelex 4.0%
The withered powder of tea (200 order) 8.0%
Starch 8.0%
Thiophene ketone (40% powder) 5.0%
The diatomite surplus.
B. manufacture process
Also weigh up various materials like clockwork by above recipe calculation.Start mixing machinery earlier with bacillus bacterium powder, jinggangmycin, the withered powder of tea, the abundant mixing of thiophene ketone.Adhesive, diatomite, starch premix are got togather, the 3rd step added fully mixing such as mixer and jinggangmycin with acidic materials and pre-mixed auxiliary agent again.To remain at last into auxiliary agent add and mixing.With pulverizing mill material is crushed to more than 200 orders.With Squeezinggranulator granulation or tablet press machine compressing tablet, particle diameter or sheet are heavy with embodiment 1.Dry good granule (or sheet) can be packed through after the assay was approved and dispatch from the factory.
Embodiment 4
Bacillus subtilis BS-208 and full fringe mixing effervescent (or effervescent tablet)
Thiophene methuroxam commodity " full fringe " by name.If with former medicine preparation, then with acetone or ethyl acetate it is dissolved, quantitative and diatomite mixing uses after drying; Use as if then directly mixing thoroughly to dry afterwards with diatomite adsorption with the preparation of 23% aqueous suspension.
A. fill a prescription
BS-208 bacterium powder (1000cfu/g) 15%
" full fringe " (pure) 1.8%
Sodium bicarbonate 8.5%
Sodium carbonate 8.5%
Citric acid 9.0%
Tartaric acid 5.2%
Polyethylene glycol 15%
Lignosite 6.0%
Sodium carboxymethylcellulose 8.0%
Azone (40% powder) 5.0%
The diatomite surplus
B. manufacture method
Accurately take by weighing various materials by various material's proportion in the prescription.Add various materials by (full fringe+diatomite+carboxymethyl cellulose)+BS-208 bacterium powder+Lignosite+thiophene ketone+citric acid+tartaric order to blender, the limit edged mixes.Fully press (Na behind the mixing again
2CO
3+ NaHCO
3+ polyethylene glycol) order adds remaining material, and fully mixing is also pulverized after Squeezinggranulator granulation or tablet press machine compressing tablet again.Particle diameter (or sheet is heavy) is with embodiment 1.Carry out product quality inspection after the flash baking, dispatch from the factory through the packing of product that is up to the standards.
Embodiment 5
Bacillus subtilis BS-208 and propiconazole mixing effervescent (or effervescent tablet)
The propiconazole trade name " is taken off Z-TEK ", " Tilt " etc.It is some quantitatively to take by weighing propiconazole, adds an amount of dissolve with methanol, with precipitated calcium carbonate absorption, makes its content more than 30% (W/W).
A. fill a prescription:
BS-208 bacterium powder (1000cfu/g) 20%
Propiconazole 5.0%
Sodium bicarbonate 6.5%
Sodium carbonate 6.5%
Citric acid 6.0%
Tartaric acid 5.5%
Polyethylene glycol 20%
Neopelex 3.0%
The withered powder of tea (200 order) 6.7%
Sodium carboxymethylcellulose 5.0%
Azone (40% powder) 6.0%
The precipitated calcium carbonate surplus.
B. manufacture method:
Calculate the consumption of various materials by the proportional meter of above-mentioned prescription, weigh up various materials like clockwork, in mixing machinery, earlier propiconazole, precipitated calcium carbonate, sodium carboxymethylcellulose, BS-208 bacterium powder are mixed, add the withered powder of citric acid, tartaric acid, neopelex and tea then, fully mixing.Add NaHCO again
3, Na
2CO
3, the limit edged mixes, and adds polyethylene glycol at last, abundant again mixing.Through Squeezinggranulator granulation or tablet press machine compressing tablet, particle diameter 1.2mm (sheet weighs 1.3 grams), air-dry.Packing is dispatched from the factory through being up to the standards.
Embodiment 6
" hundred is anti-" mixes effervescent with " full fringe "
The preprocess method of " full fringe " is with embodiment 7
A. fill a prescription:
" hundred is anti-" mattress powder (commercially available) 15%
" full fringe " (pure) 2.0%
Sodium bicarbonate 5.5%
Sodium carbonate 8.0%
Citric acid 7.0%
Tartaric acid 6.0%
Polyethylene glycol 17%
Sodium lignosulfonate 8.0%
Sodium carboxymethylcellulose 8.0%
Azone (40% powder) 6.0%
The diatomite surplus
Manufacture method:
Calculate the consumption of each material in the ratio of above-mentioned prescription, weigh up various materials like clockwork.In mixer,, add sodium lignosulfonate, thiophene ketone again, mixing earlier with " hundred is anti-", " full fringe ", diatomite, sodium carboxymethylcellulose mixing.Adding citric acid, tartaric acid then, mixing.Last power NaHCO
3, Na
2CO
3And polyethylene glycol, the limit edged mixes.Fully mixing the back pulverizes and granulation or tablet press machine compressing tablet.The particle diameter sheet is heavy with embodiment 7.Product is packed through after the assay was approved and is dispatched from the factory.
Embodiment 7
" hundred is anti-" and propiconazole mixing effervescent (or effervescent tablet)
The preprocess method of propiconazole is with embodiment 5.
A. fill a prescription:
" hundred is anti-" bacterium powder (commercially available) 15%
Propiconazole 5.0%
Sodium bicarbonate 5.0%
Sodium carbonate 8.0%
Citric acid 6.5%
Tartaric acid 6.0%
Fumaric acid 0.8%
Polyethylene glycol 20%
Neopelex 3.0%
The withered powder of tea (200 order) 8.0%
Sodium carboxymethylcellulose 6.2%
Azone (40% powder) 5%
The precipitated calcium carbonate surplus
B. manufacture method:
Calculate the consumption of various materials by the proportional meter of above-mentioned prescription, weigh up various materials like clockwork, in mixing machinery, earlier propiconazole, precipitated calcium carbonate, sodium carboxymethylcellulose, BS-208 bacterium powder are mixed, add the withered powder of citric acid, tartaric acid, fumaric acid, neopelex and tea then, fully mixing.Power Na again
2CO
3, NaHCO
3, the limit edged mixes, and adds polyethylene glycol at last, abundant again mixing.Use Squeezinggranulator granulation or tablet press machine compressing tablet after crushed, particle diameter 1.2mm (or sheet weighs 1.3 grams), air-dry.Packing is dispatched from the factory through being up to the standards.
Confirm that by the Toxicity Determination test bacillus subtilis BS-208 bacterial strain, Y3 bacterial strain, Y2-10 bacterial strain, 9-5-5 bacterial strain, " trying hard to keep " and " hundred is anti-" commodity preparation etc. are prevented and treated rice sheath blight disease with combinations such as jinggangmycin, propiconazole, thifluzamides obvious synergistic effect is arranged.Various combinations and synergistic effect thereof are listed in the table 1.
Table 1: the proportioning and the synergistic effect of combination such as bacillus subtilis and well hilllock element
| Component A (bacillus subtilis) | Component B | Weight ratio A: B (W/W) | Synergistic effect size (CTC value) |
| BS-208 | Jinggangmycin propiconazole thifluzamide | (1～180)∶(180～1) (1～200)∶(200～1) (1～200)∶(200～1) | 130～1100 230～1800 180～1350 |
| Y3 | Jinggangmycin | (1～150)∶(180～1) | 147～816 |
| The propiconazole thifluzamide | (1～180)∶(180～1) (1～250)∶(250～1) | 210～1080 165～1430 | |
| Y2-10 | Jinggangmycin propiconazole thifluzamide | (1～150)∶(180～1) (1～150)∶(180～1) (1～200)∶(220～1) | 132～779 166～917 157～880 |
| 9-5-5 | Jinggangmycin propiconazole thifluzamide | (1～120)∶(120～1) (1～100)∶(150～1) (1～150)∶(180～1) | 123～617 130～546 168～912 |
| Try hard to keep | Jinggangmycin propiconazole thifluzamide | (1～150)∶(150～1) (1～100)∶(150～1) (1～200)∶(200～1) | 130～1100 131～497 135～660 |
| Hundred is anti- | Jinggangmycin propiconazole thifluzamide | (1～150)∶(150～1) (1～100)∶(150～1) (1～250)∶(250～1) | 120～1250 126～590 134～650 |
Claims (4)
1. the effervescent agent of a bactericidal composition is characterized in that, it forms with content as follows:
Active component 0.5～50%
Disintegrant acid compound 4～25%
Alkali compounds 4～25%
Adhesive 2～30%
Surfactant 1～20%
Help and collapse agent 1～20%
The filler surplus;
Active component be bacillus subtilis with jinggangmycin with 1～180: 180～1 mix, and described bacillus subtilis strain is bacillus subtilis BS-208, Y3, Y2-10,9-5-5;
Acid compound is a citric acid;
Alkali compounds is sodium carbonate and/or sodium bicarbonate;
Adhesive is a polyethylene glycol;
Surfactant is neopelex and/or the withered powder of tea;
Help that to collapse agent be starch and/or sodium carboxymethylcellulose;
Filler is a diatomite.
2. according to the effervescent agent of claim 1, it is characterized in that, also contain the high bleeding agent of 1-15%.
3. according to the effervescent agent of claim 2, it is characterized in that high bleeding agent is selected from azone or thiophene ketone.
4. preventing and treating the application of rice sheath blight disease according to any one effervescent agent of claim 1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101352371A CN100391346C (en) | 2003-12-16 | 2003-12-16 | Effect enhanced sterilization composition and effervescence agent thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101352371A CN100391346C (en) | 2003-12-16 | 2003-12-16 | Effect enhanced sterilization composition and effervescence agent thereof |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200310121389 Division CN1268213C (en) | 2003-12-16 | 2003-12-16 | Effect enhanced sterilization composition and effervescence agent thereof |
Publications (2)
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|---|---|
| CN1802929A CN1802929A (en) | 2006-07-19 |
| CN100391346C true CN100391346C (en) | 2008-06-04 |
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|---|---|---|---|
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Cited By (2)
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| WO2013178648A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis |
| US9364006B2 (en) | 2012-05-30 | 2016-06-14 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide |
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| CN102356771A (en) * | 2011-08-16 | 2012-02-22 | 江苏省绿盾植保农药实验有限公司 | Sterilization composition and use thereof |
| EP3360418A1 (en) | 2012-05-30 | 2018-08-15 | Bayer CropScience Aktiengesellschaft | Composition comprising a biological control agent and a fungicide |
| AR091196A1 (en) * | 2012-05-30 | 2015-01-21 | Bayer Cropscience Ag | COMPOSITION THAT INCLUDES A BIOLOGICAL CONTROL AGENT AND A FUNGICIDE |
| CN103430963A (en) * | 2013-08-14 | 2013-12-11 | 南京农业大学 | Composition containing bacillus subtilis and thifluzamide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2013178648A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis |
| US9364006B2 (en) | 2012-05-30 | 2016-06-14 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide |
| US9700056B2 (en) | 2012-05-30 | 2017-07-11 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis |
Also Published As
| Publication number | Publication date |
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| CN1802929A (en) | 2006-07-19 |
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