CN100379576C

CN100379576C - Enzyme print humidification in a continuous process for manufacture of electrochemical sensors

Info

Publication number: CN100379576C
Application number: CNB2003801080944A
Authority: CN
Inventors: O·W·H·达维斯; J·T·哈尔福德; A·G·辛; R·M·尤伊达尔
Original assignee: Inverness Medical Ltd
Current assignee: LifeScan Scotland Ltd
Priority date: 2002-10-30
Filing date: 2003-10-30
Publication date: 2008-04-09
Anticipated expiration: 2023-10-30
Also published as: CN1732381A; CN100374305C; CN100465634C; CN1732093A; CN1732382A; CN1732092A; CN100531521C; CN100489513C; CN1826524A; CN100577810C; CN1732269A; CN1692148A; CN1732382B; SI1578612T1; CN1732724A

Abstract

In a web manufacturing process according to the present invention, the process includes at least one print station adapted to print enzymes on a moving substrate. In one embodiment, the web manufacturing process includes continuously moving the substrate, depositing enzyme ink onto the substrate through a screen printing process wherein ink is deposited on a top side of the screen and forced through the top side onto the substrate which is positioned adjacent to the bottom side of the screen. In order to improve ink transfer, air at the top side of the screen is humidified to a first relative humidity and air at the bottom side of the screen is humidified to a second relative humidity.

Description

The print humidification of enzyme in the continuous process of production electrochemical sensor

Technical field

The present invention relates generally to and produces improving one's methods of electrochemical sensor, more specifically, relates to improved web plate production method, and wherein serigraphy stands in and remains in the enzyme printing process under the predetermined temperature and humidity level.

Background technology

Electrochemical sensor is applied in the multiple diagnostic method, comprises the glucose of measuring blood of human body.Produce such electrochemical sensor and comprise the many small strips of production, they comprise the electrode that is arranged in the sample reception tank that is suitable for containment blood or other body fluid.Body fluid has formed the complete loops between the electrode in the pond.Electrode is covered by at least a reactant usually, and this reactant can form the intermediate analysis thing with analyte (such as the glucose) reaction in the blood, and it can be measured by being suitable for the electric current on the measurement electrode or the meter of electric weight.Produce such electrochemical sensor and need deposit which floor electrode material, insulating materials and reactant in very little space, and the accuracy of these layers and arrange that the final function for equipment is crucial.In addition, satisfy the demands, press for electrochemical sensor and under high speed, produce, and arrangement need have absolute accuracy between layer and the layer in order to reduce cost.

Electrochemical sensor can use in many application.In the application therein, the electrochemical sensor lath is inserted in the particular measurement meter that monitors such as the glucose in blood or other interstitial fluid or other analyte (such as fructosamine, hematocrit or the like).According to the design of electrochemical sensor, the layout of electrode, the reactant and the other factors of use, electrochemical sensor can be tested many analytes.Many these application, especially the lath of measuring glucose needs the outward appearance of sensor to have specific size and structure, produces thus and need carry out in the specific margin of tolerance to give electrochemical sensor with predictability and repeatable feature as much as possible.

Owing to need produce many sensors fast, and each sensor has very little pool size very little electrode need be set in close tolerance thus, and the method for producing sensor is just complicated more.When measuring blood or interstitial fluid glucose, the principal element that hinders proper testing is the pain that produces when extracting requirement blood or interstitial fluid.The pain that produces when needing greater amount is generally greater than extracting the pain that produces in a small amount.Therefore, produce that only to need more the blood of smallest number or the sensor of interstitial fluid be useful, generation pain still less so in use, thus encourage the dispersion or the continuous measurement of rule more.Need still less that a kind of method of analyte is to make to have the more electrochemical sensor of minor structure feature, hold the pond and hold very little electrode in the pond at these samples such as having littler sample.But so little feature more is difficult to preparation, and is especially just more difficult for the analysis measurement of accurate and reproducibility.

There are many methods can be used to produce electrochemical sensor, comprise rotogravure and cylinder method for printing screen.In the rotogravure method, a cylinder is capped the coating of the shape of the part (such as electrode) that limits printing.And then cylinder can be printed other film or layer (such as enzyme or insulation material layer).

By the rotogravure method conductive ink that prints electrode, can be to form one or more electrodes on the flexible web plate of polymer and produce electrochemical sensor at one.Use extremely thin China ink can obtain high-quality print definition.For printing required thicker China ink and bigger print thickness during electrochemical sensor, general use fixed flat planar Printing screen when single charging flatness layer printing electrochemical sensor.Simultaneously, also describe other method, comprised the method for utilizing the rotary printing structure to produce electrochemical sensor.

In the web plate preparation method of preparation electrochemical sensor, the substrate material web plate is by a series of printing stations.At each printing station, newly being deposited upon on the substrate or being deposited on previous for example the layer such as materials such as electrode materials with the method for printing screen deposition.In method for printing screen, web plate is arranged under filter screen and the China ink, pushes through the selected part of filter screen has predetermined arrangement in this part printing that is positioned at the web plate under the filter screen layer such as the conductive ink that is used to prepare electrode.Thus, by mobile substrate from a printing station to next printing station, make on substrate to form electrochemical sensor and become possibility, continuous each layer of printing also cuts out single sensor from the web plate of finishing.

In a kind of preparation method, transmit continuous substrate web plate and pass at least two electrode layers of printing station preparation and at least one first reactant layer.Printing station can be cylinder rotogravure station or cylinder serigraphy station.But rotogravure (rotation engraving cylinder) and cylinder serigraphy (rotating cylindrical screen cloth/sieve plate) printing process exist shortcoming when printing electrochemical sensor on web plate.The rotogravure printing generally has only very thin printing height.The thick conductive ink that is formed for the required thickness of electrode of electrochemical sensor (especially those are used to detect the sensor of blood-glucose) more may cause reduction on electrochemical sensor quality, uniformity and the reliability because of imperfect, inconsistent printing.Use the rotogravure method of carbon ink (generally have highly filled and can be very sticking) to prepare especially difficulty of carbon electrode, this is to cause between trace the imperfect, uneven of needle drawing to be filled or emptying because the solid-liquid in the China ink will separate mutually.This will cause print thickness inhomogeneous, and reduce the quality and the uniformity of carbon electrode.The cylinder method for printing screen is fit to single feed arrangement (different with continuous web plate device).In addition, the ability that influences printing quality thus of the interaction between operation screen cloth and the printed medium is owing to the cylindrical character of screen cloth is restricted.And the various sieve plates that are suitable for obtaining the accurate thickness of every type of China ink (carbon, silver/silver chlorate, insulating materials, enzyme or other reactant layer) are not to be easy to buy yet.

The electrochemical sensor that is used to measure a blood or a matter glucose also can be by the usage platform printing machine (such as can be from Kippax UK, Huddersfield, UK and Registerprint, Thieme or Svecia that London, UK obtain) the rapid printing process preparation of multistep, and by with printing flat plate substrate card be arranged in parallel can be from DEK Machinery, Weymouth, UK and BTP Craftscreen, Coventry, UK obtains sieve plate and measures China ink.The advantage of this method be can with accurately repeatably mode produce sensor, therefore can make user's comparative result at any time.The substrate boards of printing multirow lath is by having the platform printing station of a plurality of row perpendicular to traffic direction.In this preparation method, the thin layer China ink is formed a large amount of sensor strip by the order serigraphy on polymer matrix film.At first deposit carbon ink to form electrode layer.Next deposit the dielectric ink layer.The deposition reactant layer generally is the enzyme China ink then.Next deposit the second enzyme layer.Deposit adhesive layer then.But last deposit hydrophilic layer.Plate cutting is embarked on journey and then cut into single lath before protective film can be set on sensor board.The single plate of this substrate preparation method preparation can be produced the sensor strip more than 500 or 500.These sensor strip are arranged on in going by 0 to 9 of flatbed press (print direction) direction perpendicular to the substrate boards operation, and each row has 50 sensor strip.1-50 in an every row lath all is parallel to print direction.Each lath can be operated with hand between each printing station.Especially afterwards, each plate can be put into cutting mechanics with hand operated at four print steps (printed carbon ink, dielectric ink and two-layer enzyme China ink), therefore can delegation's sensor strip and cutting along every row that another row separates mutually.Next, operate the lath that every line sensor lath is cut into 50 separation.These operating procedures are time-consuming, inefficient.

Therefore need to improve the method for preparing electrochemical sensor, especially preparation is used for measuring mark such as the blood of health or the electrochemical sensor of interstitial fluid (glucose, fructosamine, hematocrit or the like).In addition need with suitable cost at a high speed, predictable, repeatably prepare the method for sensor strip.Also need in addition at a high speed, predictable and repeatably method production have the sensor strip of very little part, the lath of each completion can be used for reliable, predictable and measure the analyte of body fluid accurately in reproducible mode.

In the method for printing screen of preparation electrochemical sensor, the enzyme China ink can be about 60% water.Water content guarantees that the viscosity of China ink keeps identical in whole printing operation process in the enzyme China ink.The amount that has guaranteed to be deposited on on-chip China ink like this in printing operation is consistent.If the water content in the China ink changes the difference that will cause the enzyme printing in printing process.Moisture loses the printing precision that enzyme will be done cause difference in mesh and reduces the amount that is deposited on the China ink on the substrate surface in the enzyme China ink.

The purpose of the present invention is to be used in to prepare in the method for detection such as the electrochemical sensor of the analytes such as glucose in the body fluid such as blood.In such preparation method, need by the screen cloth deposition materials, and such material has very high water content such as enzyme, such as 60%.If deal with improperly, such material will be dry and be blocked employed screen cloth in the method for printing screen.If screen cloth is blocked, preparation process will stop, and cause bigger consumption and loss lot of materials.The web plate production method that is designed for the preparation electrochemical sensor thus is favourable, and wherein high-moisture printing material (such as China ink) can be not dry and stop up screen cloth.

Summary of the invention

According to web plate production method of the present invention, comprise that at least one is suitable for the printing station of printing enzyme on the substrate that moves.In one embodiment, the web plate production method is included in continuous mobile substrate in the entire method, deposits the enzyme China ink by method for printing screen on substrate, and its China and Mexico are deposited on the top side of screen cloth, and fall on the substrate that is positioned at contiguous screen cloth bottom side stressed this top side of passing.In order to improve the transmission of China ink, at the air saturation of screen cloth top side to first relative humidity, at the air saturation of the bottom side of screen cloth to second relative humidity.First and second relative humidity can be identical, can be in the scope such as about 85-95%.In another embodiment of the present invention, first and second relative humidity are in the scope of about 87-88%.In another embodiment of the present invention, first and second relative humidity can be about 87.69%.Can further improve method of the present invention by the heat and the humidity that are controlled at the screen cloth upper and lower surface that is used to print enzyme in the web plate production method of the present invention.Especially, between near the air themperature the screen cloth can be controlled at such as about 18 to 22 degrees centigrade.Air themperature can further be controlled at about 20.59 degrees centigrade.In addition, in screen plate printing method according to the present invention, can be by before all China inks of deposition, substrate being cooled to predetermined temperature such as the about 18 degrees centigrade methods of improving deposition enzyme China ink.

Description of drawings

New feature of the present invention is set forth in claims especially.By with reference to following detailed description to the exemplary embodiment of having used the principle of the invention, and the feature and advantage that can better understand the present invention of accompanying drawing, wherein:

Fig. 1 is the schematic diagram that shows 8 parts of screen plate printing method;

Fig. 2 A is the schematic diagram that shows first and second parts of screen plate printing method;

Fig. 2 B is the schematic diagram that shows the 3rd, the 4th and the 5th part of screen plate printing method;

Fig. 2 C is the schematic diagram that shows the 6th and the 7th part of screen plate printing method;

Fig. 3 is the schematic diagram that shows around the wet environment of the 5th and the 6th part of screen printing;

Fig. 4 is the bottom view that shows around the wet environment of the 5th and the 6th part of screen printing;

Fig. 5 is the perspective view with pipe of perforation;

Fig. 6 is the schematic diagram that shows the overflow circulation;

Fig. 7 is the schematic diagram that shows print cycle;

Fig. 8 is the schematic diagram that shows two different squeegee angles;

Fig. 9 is the schematic diagram that shows two different squeegees positions;

Figure 10 is the schematic diagram that shows screen cloth snap-on distance;

Figure 11 is the decomposition view of pretreating zone (211);

Figure 12 is the decomposition view of first dry section (217);

Figure 13 is the decomposition view of second dry section (224);

Figure 14 is the decomposition view of the 3rd dry section (230);

Figure 15 is the decomposition view of the 4th dry section (236);

Figure 16 is the decomposition view of first clean unit (240);

Figure 17 A-17D is the view of the insulation material layer on the carbon-coating of suitable registration;

When Figure 18 A-18D is the workpiece of the generation because screen cloth is stretched, the view of the insulation material layer on the carbon-coating of inappropriate registration;

Figure 19 A-19D for when workpiece when screen cloth 301 is not stretched, the view of the insulation material layer on the carbon-coating of inappropriate registration;

Figure 20 A-20D is the schematic diagram that shows the print result of the operator's registration web plate that uses the first inspection guide plate visual examination in the initial registration process;

Figure 21 A has the example that first and second web plates are checked the sensor board of guide plate; The first, second, third and the 4th Y register mark; The X register mark;

Figure 21 B is the decomposition view with the delegation in the sensor board of carbon X register mark;

Figure 21 C is the decomposition view with the delegation in the sensor board of the insulating materials X register mark on the coated carbon X register mark;

Figure 22 is the schematic diagram of the parameter X, Y and the θ that are used for the registration screen plate printing method.

The specific embodiment

Fig. 1 is the schematic diagram that shows according to 8 parts of screen plate printing method of the present invention.Part 1 is unwinder unit 101.Part 2 is a pretreatment unit 102.Part 3 is a carbon printing station 103.Part 4 is an insulating materials printing station 104.Part 5 is the first enzyme printing station 105.Part 6 is the second enzyme printing station 106.Part 7 is rewinder unit 107.Part 8 is a stamping machine 108.Although one skilled in the art will appreciate that following explanation about relating to the method and apparatus of these 8 parts, method and apparatus of the present invention can have the part of more or less quantity.Although use 4 printing stations, can use one or more printing station under the scope of the present invention not departing from such as design in this embodiment.Have minimum two printing stations in one embodiment in order to layer and the reactant layer of printing electrode.

In one embodiment of the invention, part 1 can be used such as implementing from the substrate material unwinder unit 101 of the Martin unwinder of the Martin Automatic Inc. of Rockford IL./splice automatically machine.In this embodiment of the present invention, part 2,3,4,5 and 6 can use the modified Kammann printing machine that obtains from the Werner Kammann MaschinefabrikGmbh of the b ü nde of Germany, and model is that 4.61.35 implements.In this embodiment of the present invention, part 2 can be pretreatment unit 102.Pretreatment unit 102 can be before printing preliminary treatment substrate 242, and part 3,4,5 and 6 can be used to carbon, insulating materials, first enzyme and the serigraphy of second enzyme China ink on substrate 242.Part 7 can comprise such as can be from the rewinder unit 107 of the Martin Automatic Inc. of Rockford IL..Part 8 can comprise that such as can be from Lenexa it is the Preco punch of 2024-P-40T XYT CCD CE that the Preco Press of Kansas obtains model.Although should be mentioned that the model of concrete device, those skilled in the art can understand under the condition that does not depart from the scope of the invention, and these devices can change and/or replace and/or omit fully.

Fig. 2 A, 2B and 2C are substrate 242 by the schematic diagram according to part 1～8 of screen printing process of the present invention o'clock.In one embodiment of the invention, the material that is used for substrate 242 can be a kind of polyester material (trade (brand) name Melinex  ST328), is made by Dupont TeijinFilms.Substrate 242 provides with the material of reeling, and it can be that wide, the about 660m of for example nominal 350 micron thickness, 370mm is long.The size that has been found that these thickness and width is particularly suitable for making by the electrochemical sensor of plane serigraphy on the substrate web plate.This be because make material firm so as the printing and the exercisable equipment that passes, and have enough width hold appropriate amount sensor so that this process in viable commercial.Substrate 242 can comprise the acrylic acid coatings that applies on one or both sides improve the China ink adhesion.Polyester is a preferable material, because have gratifying character under its high temperature in screen printing process of the present invention and the tension force.Though polyester, in fact Melinex is the preferred material of one embodiment of the invention, and those skilled in the art it is contemplated that according to specification record can also use other material.In fact other, those skilled in the art can also estimate the variation of material thickness, width and length, bigger width and length are provided for making the additional capacity of sensor, and the variation of material thickness helps the registration in preliminary treatment or the printing process under some conditions.In a preferred embodiment of the invention; before entering carbon printing station 103; substrate 242 is exposed to during thermostabilization handles, and heated substrate to 185 ℃ is not placed on strives under the big pulling force guaranteeing that substrate 242 is that 140-160 ℃, pulling force are to produce minimum size distortion in the screen printing process of 165N in temperature.The pulling force that adopts is generally minimum, only enough drives web plate and passes through heater.But although found to have this heating stabilization processes, the variation that still can take place from a print steps to next print steps registration causes sensor failure.Thus, before contiguous printing, introduced pre-treatment step.As explaining that after this (part 1) substrate is heated to above the temperature (being generally 160 ℃) in a back print steps in pre-treatment step.In a preferred embodiment, substrate also is maintained under the pulling force in this pre-treatment step and (is generally 165N).In this embodiment, preliminary treatment be placed on pulling force under combine greatly reduce the printing registration variation and improved final output.In one embodiment of the invention, multireel substrate 242 uses in unwinder unit 101 or rewinder unit 107 such as splicing flat paper tape from the PS-1 that Intertape Polymer Group obtains and is stitched together.

Fig. 2 A is the part 1 of the screen plate printing method of description one embodiment of the invention and the schematic diagram of part 2.In Fig. 2 A, part 1 is a unwinder unit 101.Unwinder unit 101 comprises first axle 200 and second axle, 201, the first concatenation units 202 and first accumulator 203.In Fig. 2 A, part 2 is a preprocessing station 102.Preprocessing station 102 comprises first cleaning unit 204, general obsolete second concatenation unit 205, backhaul roll 206, the second cleaning units 207, force cell 208, the first printing cylinders, 209, the first driven rollers 210 and the first siccative zone 211.

In the embodiment of the invention shown in Fig. 2 A, unwinder unit 101 be such as can comprising Martin unwinder/splice automatically machine, can be in order to easily substrate 242 is delivered in the preprocessing station 102 under the pulling force of about 80N continuously.Unwinder unit 101 comprises the first unwinding axle 200 and the second unwinding axle 201.Notice that axle also can be called mandrel.The first unwinding axle 200 keeps a volume substrate material 242, and the continuous preprocessing station 102 of substrate 242 being sent to part 2.The second unwinding axle 201 keeps the standby substrate 242 of a volume, and its substrate 242 ends that can be spliced to the first unwinding axle 200 automatically are so that the semi-continuous substrate 242 that provides to be provided.This continuous process repeats from the first unwinding axle, 200 to second unwinding axles 201.Substrate material accumulator 203 stores the substrate 242 of predetermined lengths, and (the first unwinding axle 200 and the second unwinding axle 201 are all fixed in this course) distributes the preprocessing station 102 of stored substrate 242 entering parts 2 when carrying out concatenation in first concatenation unit 202.Splicing is to dock splicing in any side at the link position place of material with the splicing tape of certain-length.In order to ensure quality, be dropped at the about 10 meters printed substrate of any side that splices.The first unwinding axle 200 and the second unwinding axle 201 comprise web plate edge guide (not shown), and guiding substrate enters first concatenation unit 202.The web plate edge guide is suitable for preventing that substrate 242 is being fed to first concatenation unit, 202 hour offsets.

Usually, machine of the present invention is set and produces 2 to 10 volumes at any time simultaneously, and more frequent is 6 volume substrates.Be connected to the printing station that China ink provides the source continuously for these, the usage quantity of volume generally is not a problem.But, for two enzyme printing stations, the quantity that provides of limit ink, the usage quantity of volume is an important input parameter.The usage quantity of volume must determine to be placed on the China ink amount on the screen cloth before printing engineering begins.Such as using 6 volume operations, before part 5 and part 6 beginning printing operations, the suitable enzyme China ink amount of 6 volumes (perhaps more than 6 volumes) need placed on the screen cloth.Thus, whole printing operation process need has enough enzyme China inks to guarantee the printing enzyme in whole printing operation unanimity in the cycle.Near enzyme printing station screen cloth, place a wall and need in an operation process, screen cloth not filled it up with at the enzyme China ink that adds sufficient amount on the screen cloth guaranteeing, reduce the enzyme China ink and overflow and be sprinkled upon the on-chip risk of moving screen cloth under of web plate from screen cloth.

In one embodiment of the invention, substrate 242 remains in whole process under the pulling force of about 165N needing to keep 4 layers the registration (printing registration tolerance usually is 300 μ m) of printing.Thereby substrate 242 also can be placed under the temperature below 140 ℃ or 140 ℃ dry printer's ink in each print steps.Because such pulling force and temperature makes substrate that the trend that stretches or expand be arranged in processing procedure, the result exceeds registration thing error.Really, the variation of dimension of picture, all is difficult to expect from a printing process to another printing process and each printing process to another printing station from a printing station, and can not be too high.

In the embodiment of the invention shown in Fig. 2 A, part 2 is a pretreatment unit 102, and preliminary treatment occurs in any graphic printing before on the matrix.The amount of preliminary treatment substrate 242 in the step in succession of screen printing process to reduce expansion and to stretch, the registration when also helping substrate 242 through part 3-6.Preprocessing station can heated substrates 242 to the temperature that is not surpassed by follow-up print steps.General its occurs in 150N under the pulling force of 180N, and more typical is about 165N.Yet, in other embodiments, pretreatment unit 102 can heated substrate 242 to the temperature of enough from substrate 242, removing non-response elongation, also can be chosen under the above-mentioned pulling force and carry out.

In one embodiment of the invention, substrate is heated to about 160 ℃ at pretreating zone 211, as being shown specifically in Figure 11.As explained above, in one embodiment of the invention, the temperature that substrate is heated in preprocessing station 102 is not forereached by the subsequent operation of substrate 242, comprises the subsequent drying step.Follow-up printing process can be by providing big slightly template screen mesh size (being generally 750 μ m on the direct of travel of web plate) compensation because the big slightly figure that preprocessing station 102 stretches and causes.It can be chance that new screen cloth is provided.Can therefore change in other parameter of each printing station, so that adapt to the variation of dimension of picture, and not need to change screen cloth, for example the relative velocity of screen cloth and web plate.But, change the existence restriction for adaptable feature size.Its preferred preliminary treatment substrate as described herein thus reduces the increase of overall dimension of picture and reduces variation in described dimension of picture increases.

In one embodiment of the invention, preprocessing station 102 also comprises additional element, the existing in fact effect that is beneficial to according to the proper operation of web plate production process of the present invention.In pretreatment unit 102, have two web plate cleaning units, i.e. first cleaning unit 204 of the upper and lower of cleaning substrate 242 and second cleaning unit 207.First cleaning unit 204 and second cleaning unit 207 use the roller of adhesive coating to remove particulate matter from substrate 242 before any print steps.First cleaning unit 204 can be for example can from buy at the KSM of Glasgow, United Kingdom Web Cleaners, model is the WASP400 cleaner.Second cleaning unit 207 can be the cleaner of for example buying from Teknek.Pretreatment unit 102 further comprises a backhaul roll 206 and a load cell 208.Backhaul roll 206 is used to control the pulling force (the especially pulling force between backhaul roll 206 and output roll 238) of substrate 242.Backhaul roll 206 links to each other with load cell 208 via a control system (not shown).Substrate 242 leaves the second enzyme printing station 106 in the part 6 with constant rate of speed under the effect of output roll 238.Load cell 208 in part 2 is measured when substrate 242 and is passed screen printing process of the present invention pulling force on it when moving.Backhaul roll 206 is regulated its speed to be controlled at the pulling force of pre-position.In the web plate production process of the present invention typical substrate pulling force be about 150N to 180N, more particularly 160N is approximately 165N in the present embodiment to 170N.

Fig. 2 B is depicted as the schematic diagram of part 3, part 4 and part 5 according to screen printing process of the present invention.In Fig. 2 B, part 3 is carbon printing stations 103.Cleaning systems (buying from Meech) were installed before printing, and described cleaning systems use the upside (printed side) and the downside of a vacuum and brush system cleaning substrate, and upper brushes and vacuum station 251 and bottom brush and vacuum station 250 offset with respect to each.Upper brushes and vacuum station 250 just contacted with substrate before sharp cooling roll 212 and accumulator 213, and were nearest accessibility points before the carbon printing.The bottom brush contacts with substrate after substrate leaves pretreatment unit 102 immediately with vacuum station 251.Carbon printing station 103 comprises first sharp cooling roll 212, second accumulator 213, second print roller 214, first imageing sensor 215, second driven roller 216, first dry section 217 and second sharp cooling roll 218.In the embodiment of the invention shown in Fig. 2 B, part 4 is insulating materials printing stations 104.Insulating materials printing station 104 comprises the 3rd sharp cooling roll 219, the 3rd accumulator 220, the 3rd print roller 221, second imageing sensor 222, at a Y registration arrangement (not shown), the 3rd driven roller 223 and second dry section 224 of position 237A.In Fig. 2 B, part 5 is first enzyme printing stations 105.The first enzyme printing station 105 comprises the 4th sharp cooling roll 225, the 4th accumulator 226, the 4th print roller 227, the 3rd imageing sensor 228, the 2nd Y registration arrangement, at the 237B (not shown), and 4 wheel driven action roller 229 and the 3rd dry section 230.

In process according to the present invention, the part 3 of web plate production process is the position of carrying out the carbon printing.Certainly, can understand, can change the quantity and the type of printing process, and do not break away from the scope of maximum of the present invention as those skilled in the art.For example, can provide two carbon printings or one or more printing with carbon and metallic particles, silver/silver chlorate China ink or gold or palladium base China ink can be used for providing an electrode layer in the electrochemical sensor.The composition of insulating materials and reagent layer, sedimentary sequence, deposit thickness and other parameter that designs and it will be apparent to those skilled in the art that of arranging also can change with respect to embodiment described here.In part 3, the carbon pattern that is used for electrochemical sensor constructed in accordance can use serigraphy to print.The basic module of carbon printing station 103 is shown in Fig. 6 and 7.Especially, suitable printing station according to the present invention comprises screen cloth 301, print roller 303, print roller 600, overflow scraper 603, squeegee retainer 605 and squeegee 606 down.In carbon printing station 103, print roller 600 is second print roller 214.Screen cloth 301 is generally flat structure, and is typically the silk screen that comprises the egative film that is used to provide required pattern.Carbon ink is applied on the silk screen and is pushed into when printing and passes this silk screen.In this stage, flat screen cloth can pass under the pulling force of screen template in the weight of China ink and the promotion China ink of squeegee, outside flat pattern, deform a little (for especially true in the enzyme print steps, employed whole China ink is deposited on the screen cloth when printing process begins usually in whole printing process).

Overflowing in the cyclic process according to of the present invention, by mobile squeegee 606 on corresponding to the first direction 608 of the web plate of substrate 242 motion, overflow scraper 603, print roller 600 and following print roller 303, in screen cloth 301, be full of black 604.Screen cloth 301 moves along the second direction 607 opposite with the first direction 608 of substrate 242, and China ink 604 is added on the screen cloth 301.

In follow-up print cycle according to the present invention, as shown in Figure 7, squeegee 606 is sent to China ink 604 on the substrate 242 by screen cloth 301.In print cycle, squeegee 606, overflow scraper 603, print roller 600 and following print roller 303 and all on the second direction 607 opposite, move with the web plate moving direction of substrate 242.Move on the first direction 608 of screen cloth 301 web plate moving direction of substrate 242 in corresponding to print cycle, its China and Mexico 604 are pushed by screen cloth 301 and are deposited on the substrate 242.During print cycle, screen cloth 301 moves with identical with substrate or very approaching identical speed in the direction identical with the web plate substrate thus.Screen cloth 301 is flat substantially when static, but in use, and it is promoted and slight distortion takes place towards web plate by squeegee 606, but can be returned to original-shape basically after squeegee 606 is removed.When the China ink 604 of being reloaded was prepared next print cycle, screen cloth 301 moved along the direction with the substrate reverse movement then.When China ink was loaded on the screen cloth 301, the very slight screen cloth that makes of weight of China ink bent.When leaving printing station, the direction 608 that screen cloth 301 and web plate move at an angle.This setting (this angle is generally about 10-30 degree, can be about 15 degree more specifically) has improved China ink and has been discharged on the substrate from screen cloth, thereby has improved print definition and repeatability.The distance of the angle of screen cloth and substrate, the angle of squeegee, screen cloth and squeegee, squeegee can be used for controlling and optimizing the final precision and the uniformity (it is more detailed that the embodiment of a screen printing mechanism describes, and this piece document is combined in here by reference) of whole card in the U.S. Pat 4245554 of authorizing with respect to the pressure of the relative velocity of position, snap-on distance (snap distance), substrate and the screen cloth of printing cylinder and squeegee.

Especially, in carbon printing station 103, described China ink is a carbon ink.A kind of example of suitable carbon ink will be described below.In an embodiment of the present invention, using squeegee 606 transmission China inks 604 by screen cloth and before being printed on the substrate 242, screen cloth 301 usefulness China ink 604 fills up.The printing carbon figure that is deposited on the substrate 242 carries out drying then, drying means directly blows 140 ℃ hot-air method on the substrate print surface such as using in first dry section 217 with the oven dry group of 4 separation, illustrated in fig. 12 more detailed.

But use at the carbon printing station that suitable China ink includes, but not limited to have metallic particles carbon, silver/silver chlorate, conduction printer's ink auri, the palladium base.

Can use the conductive ink of other type, but for example have metallic particles carbon, silver/silver chlorate, conduction printer's ink auri, the palladium base.

In one embodiment of the invention, before the carbon printing operation and just after drying, make substrate 242 by the first Quench cylinder 212, the described first Quench cylinder is designed to cool off fast substrate 242 to predetermined temperature, be generally room temperature (approximately 18-21 ℃, be generally 19.5+/-0.5 ℃).In an embodiment of web plate production method according to the present invention, surperficial about 18 ℃ of the first Quench cylinder 212.The first Quench cylinder 212 can use such as about 7 ℃ factory's water quench to suitable temperature.The temperature that can control this cylinder by the flow velocity and/or the temperature of control factory cooling water.After printing carbon pattern was deposited in typography, substrate 242 was by the second Quench cylinder 218.It is useful reducing the temperature of substrate 242 and keeping the temperature of substrate 242, and this is because colder temperature has reduced printing process China and Mexico in possibility dry on the screen cloth and form piece on web plate.In web plate production method, use the Quench cylinder to also have another benefit,, reduce registration problems and be in operation and adjust operation to proofread and correct the needs of this problem because it can reduce the span of substrate 242 according to the present invention.

In one embodiment, the backfeed loop of the temperature by measuring the Quench cylinder and the control current/temperature temperature of controlled chilling cylinder dynamically.Those skilled in the art it is contemplated that the method for other chilling roll by embodiment described herein, such as electronic cooling unit.

In the method according to the invention, the part 4 of web plate production method is carried out the insulating materials printing.In part 4, the insulating materials figure that is used for the electrochemical sensor of the present invention's production adopts the method for printing screen that is generally flat screen to print.The primary element of insulating materials printing station 104 is shown in Fig. 6,7.Especially, suitable printing station according to the present invention comprises screen cloth 301, printing cylinder 303, printing cylinder 600, overflow scraper 603, squeegee retainer 605 and squeegee 606 down.In insulating materials printing station 104, printing cylinder 600 is the 3rd printing cylinder 221.

Overflowing in the cyclic process according to of the present invention, by mobile squeegee 606 on corresponding to the first direction 608 of the web plate of substrate 242 motion, overflow scraper 603, print roller 600 and following print roller 303 and in screen cloth 301, be full of black 604.Screen cloth 301 along with overflow cyclic process in the opposite second direction 607 of the first direction 608 of substrate 242 move, China ink 604 is added on the screen cloth 301.

In according to the print cycle below of the present invention, as shown in Figure 7, squeegee 606 is sent to China ink 604 on the substrate 242 by screen cloth 301.During print cycle, squeegee 606, overflow scraper 603, print roller 600 and following print roller 303 and all on the second direction 607 opposite, move with the web plate moving direction of substrate 242.Move on first direction 608 directions of the web plate moving direction of screen cloth 301 substrate 242 in corresponding to print cycle, its China and Mexico 604 are pushed by screen cloth 301 and are deposited on the substrate 242.It is more detailed that the embodiment of a screen printing mechanism describes in the U.S. Pat 4245554 of authorizing, and this piece document is combined in here by reference.

In the printing of removable flat screen, common flat screen has an element that moves along the direction identical with substrate and roughly the same speed in printing process.General, in each printing station, when screen cloth and substrate move away printing station, become an acute angle (among Fig. 6 shown in the A) with substrate for dull and stereotyped screen cloth substantially.It is the size of printed pattern on the directions X that the relative velocity of change substrate and screen cloth can change along the substrate direction of motion.

The template sieve plate that uses in each printing station generally comprises elastically deformable polyester or stretching and is attached at steel mesh on the rigid frame.An embodiment has used by DEKMachinery, the polyester screen that Weymouth, UK provide.Mesh is covered and is exposed under the UV light source with a film positive screen cloth by ultraviolet ray (UV) sensitive coating, and expansion and drying are so that coating drying on screen cloth forms required wiring figure.Under the help of squeegee, the open area of China ink by template, and be deposited on the substrate (China ink on substrate, form just as).Framework is for installing the device of screen cloth, bears the power that the stretching screen cloth applies and produces minimum distortion and bear the additional force that produces simultaneously in printing process.

Especially, in insulating materials printing station 104, described China ink is a dielectric ink.A kind of example of suitable insulation China ink illustrates below.In an embodiment of the present invention, using squeegee 606 transmission China inks 604 by screen cloth and before being printed on the substrate 242, screen cloth 301 usefulness China ink 604 fills up.The printing insulating materials pattern that is deposited on the substrate 242 carries out drying then, drying means directly blows 140 ℃ hot-air method on the substrate print surface such as using in second dry section 224 with the oven dry group of 4 separation, is shown in further detail in Figure 13.Example at the operable suitable China ink of insulating materials printing station in the web plate production method of the present invention is Ercon E6110-116 Jet Black Insulayer Ink, can be from Ercon, and Inc obtains.In one embodiment of the invention, the insulating materials pattern can use technology described herein in directions X (along machine) and Y direction (passing machine) and carbon figure registration.Those skilled in the art are according to describing the dielectric ink that can envision operable other type herein.In addition, can take different layers and different layer orders that the layer of different order is provided, so can produce the electrochemical sensor of different structure.

In one embodiment of the invention, before the insulating materials printing operation and just after drying, make substrate 242, comprise that printing carbon and insulating pattern are by the 3rd Quench cylinder 219, described the 3rd Quench cylinder is designed to cool off fast substrate 242 to predetermined temperature, be generally room temperature (approximately 17-21 ℃, be generally 19.5+/-0.5 ℃).In an embodiment of web plate production method according to the present invention, surperficial about 18 ℃ of the 3rd Quench cylinder 212.The 3rd Quench cylinder 219 can use such as about 7 ℃ factory's water quench to suitable temperature.It is useful reducing the temperature of substrate 242 and keeping the temperature of substrate 242, because colder temperature has reduced printing process China and Mexico in possibility dry on the screen cloth and form piece on web plate.In web plate production method, use the Quench cylinder to also have another benefit,, reduce registration problems and be in operation and adjust operation to proofread and correct the needs of this problem because it can reduce the span of substrate 242 according to the present invention.

In technical process according to the present invention, take place in the part 5 during first enzyme is printed in the web plate production method.In part 5, the enzyme of the electrochemical sensor that goes out produced according to the invention China ink pattern uses serigraphy and movably flat basically screen cloth as previously described to print.The basic composition of the first enzyme printing station 105 is shown in Fig. 6 and 7.Especially, suitable printing station according to the present invention comprise screen cloth 301, down print roller 303, print roller 600, overflow scraper 603, squeegee retainer 605, squeegee 606.In the first enzyme printing station 105, print roller 600 is the 4th print roller 227.

In follow-up print cycle according to the present invention, as shown in Figure 7, squeegee 606 transmits China ink 604 and passes on the screen cloth 301 arrival substrates 242.During print cycle, squeegee 606, overflow scraper 603, print roller 600 and following print roller 303 all with the second direction 607 of the web plate reverse movement of substrate 242 on move.Screen cloth 301 motion on corresponding to the first direction 608 of the web plate direction of motion of the substrate 242 that is used for print cycle, here China ink 604 is pushed and is passed screen cloth 301 and deposit on the substrate 242.An embodiment of screen printing mechanism has more detailed description in the United States Patent (USP) 4245554 of authorizing, be incorporated herein it as a reference.

Especially, in the first enzyme printing station 105, the China ink of being discussed is the enzyme China ink.An example of suitable enzyme China ink is listed below.In this embodiment of the present invention, screen cloth 301 is filled with China ink 604 before passing screen cloth and being applied on the substrate 242 using squeegee 606 to transmit China ink 604.Then the enzyme pattern that is deposited on the substrate 242 printing is carried out drying, the lip-deep for example 50 ℃ of hot-airs down that use the guiding substrate to be printed, two the independent drying tables of use in the 3rd dry section 230 are shown in further detail in Figure 14.An example of the suitable China ink that is used for the first enzyme printing station 105 in web plate production process according to the present invention is summed up at table 2.

Table 2

Component	Supplier
Component	Supplier	Glucose oxidase	Biozyme Laboratories
Trisodium citrate	Fisher Scientific	Glucose oxidase	Biozyme Laboratories
Trisodium citrate	Fisher Scientific	Citric acid	Fisher Scientific
Polyvinyl alcohol	Sigma Aldrich	Citric acid	Fisher Scientific
Polyvinyl alcohol	Sigma Aldrich	Hydroxyethylcellulose (Nat 250 G)	Honeywell and Stein BDH/Merck LTD Sigma-Aldrich Chemical Co.，UK
Six cyanogen close the sour potassium of iron (III)	Norlab Instruments Ltd.，UK	Hydroxyethylcellulose (Nat 250 G)
Six cyanogen close the sour potassium of iron (III)	Norlab Instruments Ltd.，UK	DC 1500 defoamers	BDH/Merck Ltd
Cab-O-sil	Ellis and Everard Ltd	DC 1500 defoamers	BDH/Merck Ltd
Cab-O-sil	Ellis and Everard Ltd	PVPVA	ISP Company Ltd
Analytically pure water	BDH/Merck Ltd	PVPVA	ISP Company Ltd

In one embodiment of the invention, after after the insulating materials printing process and just after drying, the substrate 242 that comprises the carbon of printing and insulating materials pattern is through the 4th Quench cylinder 225, and described the 4th Quench cylinder design is used for cooling off fast substrate 242 to the predetermined temperature that is generally room temperature (about 17-21 ℃ and be typically 19.5 ℃+/-0.5 ℃).In an embodiment of web plate production process according to the present invention, the surface of the 4th Quench cylinder 225 is approximately 18 ℃.The 4th Quench cylinder 225 for example can use, and the industrial colling about 7 ℃ is cooled to suitable temperature.It is useful reducing the temperature of substrate 242 and keeping the temperature of substrate 242, because lower temperature has reduced China ink in possibility dry on the screen cloth and that lump in the web plate eye.According to the present invention, the application of Quench cylinder in the web plate production process also is useful, because it has dwindled the elongation of substrate 242, reduced registration problems and in operation for compensating the needs that such problem changes process.

In addition, owing to the high moisture content of enzyme China ink with because screen cloth moves the air-flow of generation, guarantee that the enzyme China ink is not crucial at the screen cloth inner drying.Flowing relatively of the air that the screen cloth that moves runs into makes China ink dry on screen cloth, its mode is different from observed mode in flat screen printing machine (for example Thieme flat screen printing machine) usually, because screen cloth itself does not move in machine, and is different with the present invention.Not only the Quench cylinder is by guaranteeing that substrate was cooled to about 18 ℃ and alleviates these problems before enzyme screen cloth-print steps, the screen cloth of load enzyme China ink in printing process also by humidifying.In one embodiment, humidifying is continuous basically.Can be to upside, downside and/or the side humidifying of screen cloth, also can the three humidifying together.Plumbing installation provides the humidifying air stream of substantial constant respectively at upside, downside and the side of screen cloth, guarantee that the water content in the China ink remains on constant level.The appropriate device of humidifying that provides for screen cloth upside, downside and/or side according to the present invention is shown in Fig. 3,4 and 5.The quantity of humidification device (being generally the pipeline of transmission humidifying air) and being provided with depends on the humidity of water content, surrounding air of the amount that needs humidifying, China ink and temperature, temperature, screen mesh size and the screen cloth exposed amount in the environment (not humidifying air) around of temperature, the printing cylinder of substrate when near the enzyme printing station.In one embodiment, comprise the row or the pipeline 304 in multiple row hole 400 in sieve plate stroke back and forth along whole sieve plate downside transmission humidifying air.Carry humidifying air stream 300 and 304 (see figure 4)s in the above and to the pipeline (not shown) of operator's one side of machine.

Typically, the required whole enzyme China inks of print run are placed on the screen cloth when print run begins or before beginning.Because the enzyme China ink comprises most of water (be typically between 55 to 65% percentage by weights, more typical is that percentage by weight is about 60%), this China ink tends to be dried during the continued operation of running.This risk can be minimized by carry out humidifying around the screen cloth that supports the enzyme China ink.Another kind of optional mode or more typical be can make substrate before enzyme (perhaps any) printing station by using Quench cylinder as described herein to cool off.The temperature of general substrate is controlled in is less than or equal to room temperature.But the temperature of substrate remains on the dew point of room atmosphere.If indoor humidity is 60%, dew point may be 15 ℃ so.Be lower than this temperature if the temperature of substrate drops to, on substrate, condensation can take place so, the various follow-up printing process of potential infringement, the water miscible China ink of especially any use is the follow-up printing process of enzyme China ink for example.Therefore the control of substrate temperature for example between the limit value of room temperature and dew point, can be described as very important for the printing process of a success.In the control substrate temperature, control is very important through the temperature and/or the time of Quench cylinder 212,219,225 and 231.A feedback control loop can be used for measuring the temperature of substrate, for example corresponding to the temperature of room temperature and/or dew point (given indoor humidity) with the temperature of controlled chilling cylinder with substrate leaves the Quench cylinder and temperature during near next printing station.

Fig. 2 C represents is schematic diagram according to the part 6 and the part 7 of screen printing process of the present invention.In Fig. 2 C, part 6 is the second enzyme printing station 106.The second enzyme printing station 106 comprises the 5th Quench cylinder 231, the 5th accumulator 232, the 5th print roller 233, the 4th imageing sensor 234, the 5th driven roller 235, the 5th dry section 236, Y registration arrangement 237 and output roll 238.In the embodiments of the invention shown in Fig. 2 C, part 7 is rewinder unit 107.Rewinder unit 107 comprises steering mechanism 239, the first recoil axle, the 240 and second recoil axle 241.

In a process according to the present invention, the part 6 of screen printing process is to carry out second enzyme printing part.In part 6, the enzyme China ink pattern that is used for electrochemical sensor constructed in accordance prints by serigraphy.The purpose that applies two enzyme China ink coatings is to guarantee complete coated carbon electrode, makes electrode average substantially, does not have blank space.The basic composition of the second enzyme printing station 106 is shown in Fig. 6 and 7.Especially, suitable printing station according to the present invention comprise screen cloth 301, down print roller 303, print roller 600, overflow scraper 603, squeegee retainer 605, squeegee 606.In the second enzyme printing station 106, print roller 600 is the 5th print roller 233.

Overflowing in the cyclic process according to of the present invention, by mobile squeegee 606 on corresponding to the first direction 608 of the web plate direction of motion of substrate 242, overflow scraper 603, print roller 600 and following print roller 303 and in screen cloth 301, be full of China ink 604.Screen cloth 301 motion on the second direction 607 opposite with the first direction of the substrate 242 that is used to overflow circulation, at this moment China ink 604 is filled on the screen cloth 301.

In follow-up print cycle according to the present invention, as shown in Figure 7, squeegee 606 transmits China ink 604 and passes on the screen cloth 301 arrival substrates 242.During print cycle, squeegee 606, overflow scraper 603, print roller 600 and following print roller 303 all with the second direction 607 of the web plate reverse movement of substrate 242 on move.Screen cloth 301 motion on corresponding to the first direction 608 of the web plate direction of motion of the substrate 242 that is used to overflow circulation, here China ink 604 is pushed and is passed screen cloth 301 and deposit on the substrate 242.It is more detailed that the embodiment of a screen printing mechanism describes in the U.S. Pat 4245554 of authorizing, and this piece document is combined in here by reference.

Especially, in the second enzyme printing station 106, the China ink of being discussed is the enzyme China ink.In this embodiment of the present invention, screen cloth 301 is filled with China ink 604 before passing screen cloth and being applied on the substrate 242 using squeegee 606 to transmit China ink 604.Then the enzyme pattern that is deposited on the substrate 242 printing is carried out drying, the lip-deep for example 50 ℃ of hot-airs down that use the guiding substrate to be printed, two the independent drying groups of use in the 4th dry section 236 illustrate in Figure 15 in further detail.The example of the suitable China ink that is used for the second enzyme printing station 106 is identical with the enzyme China ink that is used in the first enzyme printing station described in the above-mentioned table 2.

In one embodiment of the invention, after the second enzyme printing process and just after drying, the substrate 242 that comprises the carbon, insulating materials of printing and enzyme China ink pattern is through the 5th Quench cylinder 231, and described the 5th Quench cylinder design is used for cooling off fast substrate 242 to predetermined temperature.In an embodiment of web plate production process according to the present invention, the surface of the 5th Quench cylinder 231 is approximately 18 ℃.The 5th Quench cylinder 231 for example can use, and the industrial colling about 7 ℃ is cooled to suitable temperature.It is useful reducing the temperature of substrate 242 and keeping the temperature of substrate 242, because lower temperature has reduced China ink in possibility dry on the screen cloth and that lump in the web plate eye.The application of Quench cylinder in the web plate production process also is useful according to the present invention, because it has dwindled the elongation of substrate 242, reduced registration problems and in operation for compensating the needs that such problem changes process.

In addition, owing to the high moisture content of enzyme China ink with because screen cloth moves the air-flow of generation, guarantee that the enzyme China ink is not crucial at the screen cloth inner drying.Not only the Quench cylinder is by guaranteeing that substrate was cooled to 18 ℃ and alleviates these problems before enzyme serigraphy step; the air draught of humidifying also is provided in the screen cloth upper and lower by the humidifying of upside and/or downside and/or side screen cloth, guarantees that the water content of China ink remains on constant level.The air constant ground of general humidifying flows through screen cloth.According to the appropriate device that provides humidifying for sieve plate upside and downside of the present invention as shown in Figure 3.

The second enzyme printing station 106 can comprise output roll 238, is used to check the check system 237 of registration, at the 3rd Y registration arrangement (not shown) and the bar code station (not shown) at 237C place.Output roll 238 helps the pulling force (the particularly pulling force between backhaul roll 206 and the output roll 238) of control substrate 242.Substrate 242 is removed from the second enzyme printing station 106 with constant speed under 238 effects of output roll.Y registration arrangement (not shown) at 237A, 237B and 237C place, by using a Y register mark 2101, the 2nd Y register mark 2102, the 3rd Y register mark 2103, the 4th Y register mark 2104 shown in Figure 21 A, the Y registration (promptly passing web plate) of each print cycle during the control printing.In one embodiment of the invention, a Y register mark 2101, the 2nd Y register mark 2102, the 3rd Y register mark 2103 and the 4th Y register mark 2104 can correspond respectively to the Y registration of carbon printing station 103, insulating materials printing station 104, the first enzyme printing station 105 and the second enzyme printing station 106.Each Y register mark comprises 2 triangles, and it is constituting an approximate rectangular direction and is putting.In one embodiment, the Y registration arrangement that is positioned at 237A, 237B and 237C position can lead to the Eltromat DGC650 that use buys from the Eltromat Gmbh of German Leopoldshoehe and implement.

In one embodiment of the invention, check system 237 can be used the Eltromat check system, and model is PC3100HD, and it can be bought from the EltromatGmbh of German Leopoldshoehe.This check system 237 has image component, inspection as Figure 17 A to the register mark shown in 19D and/or Figure 20 D, and can be as evaluation sensor plate 2106 instrument of defective (for example by the check result of record) whether with respect to bar code in a database.

Registration problems on the Y direction (it can change by the registration arrangement (not shown) that is positioned at 237A, 237B and 237C position during printing and/or check by use check system 237 after all printing stage has all been finished) can belong to the change of web plate pulling force or the unbalanced distortion of substrate 242.In one embodiment of the invention, can be below the bar code station comprises at parts bar code printing machine (the model A400 of commercial acquisition, Domino UK Ltd. purchase from Britain Camb), bar code horizontal system (the ScottishRobotic Systems of Perth prefecture, Scotland) and bar-code reader (at Canton, the RVSI AcuityCiMatrix of MA).Bar code station (not shown) is stamped label with two-dimensional bar code with every row of sensor board 2106.Like this for every line sensor provide unique identification code, batch/batch Digital ID, sensor board number and line number.The bar code station is also read bar code immediately and is correctly printed with the verification bar code and provide visual indication to machine operation person after printing.Bar code and be stored in the database from the procedural information of part 2 to 6, and be used to for follow-up process the identification of card and thereupon discarded/accept.

Rewinder unit 107 comprises that for example Martin recoils system automatically.This is the last part of machine, and substrate 242 is recoiled continuously.Rewinder unit 107 comprises that first recoils the axle 240 and second recoil axle 241.First recoils axle 240 keeps the volume of a substrate 242 and pulls out material from the second enzyme printing station 106 constantly.Second recoils axle 241 keeps the lay-by material volume, and its volume at substrate 242 is recoiled from first and automatically the volume of first substrate 242 is spliced to second volume after axle 240 is finished.Recoil axle 240 to second from first and recoil axle 241 these continuous processes of repetition.The flying splice that is carrying out when substrate 242 is still moving makes substrate 242 to recoil continuously.Splicing place is placed directly on the new volume of the substrate material 242 that has the bilateral pressure sensitive adhesives in advance.

Fig. 3 is the schematic diagram of the wet environment around the 5th and the 6th part of screen printing.The basic composition that is used to provide screen printing environment humidifying mode as shown in Figure 3, it comprises top humid air 300, screen cloth 301, bottom humid air 302, print roller 303, the pipeline 304 that comprises a plurality of perforation 400, substrate 242 and the 4th print roller 227 or the 5th print roller 233 down.Set humidifying and temperature with the character of attempting and guaranteeing the enzyme China ink overflow with print cycle during, the change of obvious degree does not preferably take place during print run whole in time.Especially, desirable be the viscosity of enzyme China ink and water content overflow with print cycle during, preferably during print run whole, do not change in time.The enzyme China ink contains about 63% water.The amount that constant water content assurance is applied to the China ink on the substrate 242 is consistent.If the water content of China ink changes in printing process, this can cause the change of enzyme layer thickness.In addition, the loss of moisture will cause enzyme dry on screen cloth 301 in the enzyme China ink, causes bad print definition and the amount that is applied to the China ink on the substrate 242 to reduce.Humid air in the first enzyme printing station 105 or the second enzyme printing station 106 remains on 85 to 95% relative humidity.Top humid air 300 and bottom humid air 302 are pumped into the both sides of screen cloth 301 to keep required relative humidity.Side pipe 305 is arranged on a side of web plate, and introduces the air of humidifying on a side direction web plate of next-door neighbour's enzyme printing station.Can change the character of humidification device and type with the size and dimension that adapts to printing station with for certain type of humidifying needs under certain environment of certain printing station of China ink.Often can use a cover that the upside of screen cloth and/or downside are encapsulated so that the air of humidifying can deliver in the cover with the screen cloth direct neighbor, and owing to the existence of cover remains near the screen cloth.If the cover be installed on the upper frame of screen cloth, as typical situation, cover can x direction (print direction) have a slit so that squeegee overflowing normally/print cycle in respect to screen movement.

Fig. 4 is the schematic diagram of the 5th and the 6th part wet environment on every side of screen printing.Be used to provide the screen printing environment the humidifying mode basic composition also as shown in Figure 4, it comprises top humid air 300, screen cloth 301, bottom humid air 302, has the pipeline 304 of perforation and the side pipe (not shown) at 400,305 places of boring a hole.Pipeline 304 with a plurality of perforation 400 be positioned at screen cloth 301 below, keep the viscosity of the enzyme China ink on the screen cloth 301 as the mode that is used to blast bottom humid air 302.Fig. 5 is the schematic diagram of the pipeline 304 with perforation 400 of humid air 302 bottom blasting.

Fig. 8 is the schematic diagram of 2 different squeegee angles, comprising substrate 242, print roller 600 and squeegee 606.The angle 800 that can change squeegee makes the accuracy optimization of printing zone.In one embodiment of the invention, the angle of squeegee can+/-1 to 2 degree preferred for 15+/-5.It should be noted that squeegee 606 is identical with the contact point of print roller 600 for each squeegee angle 800.

Fig. 9 is the schematic diagram of 2 different squeegee positions, comprising substrate 242, print roller 600, following print roller 303, squeegee 606, the first squeegee position 900 and the second squeegee position 901.The squeegee position is the position of squeegee with respect to the center of print roller 600.The squeegee position can produce main influence to the thickness of printer's ink.Can change the accuracy optimization that the squeegee position makes printing zone.

Figure 10 shows the schematic diagram of screen cloth snap-on distance (1000), comprising substrate 242, print roller 600, following print roller 303 and screen cloth 301.In one embodiment of the invention, screen cloth snap-on distance (1000) is the minimum distance between screen cloth 301 and the substrate 242.In a preferred embodiment of the invention, screen cloth snap-on distance (1000) is approximately 0.7mm.If it is too high that screen cloth snap-on distance (1000) is provided with, squeegee 606 can not make screen cloth 301 enough upsets with enough print definition China ink 604 is sent on the substrate 242.If it is low that screen cloth snap-on distance (1000) was provided with, screen cloth 301 can cause the accuracy of printing not enough from the print cycle of the front China ink 604 of erasing.

Figure 11 shows that the decomposition view of pretreating zone 211, it comprises first driven roller 210, hot plate 1100, first warm table 1101, second warm table 1102 and the 3rd warm table 1103.In one embodiment of the invention, the not contacts side surfaces of printing of hot plate 1100 and substrate 242.In a preferred embodiment of the present invention, hot plate 1100 can be coated with polytetrafluoroethylene (PTFE) (Teflon), and can be heated to about 160 ℃.In one embodiment of the invention, first warm table 1101, second warm table 1102 and the 3rd warm table 1103 bloat the hot-air about 160 ℃.Can understand as those skilled in the art, can change with the type that adapts to substrate and/or temperature in thickness and/or any preliminary treatment and/or the subsequent process these conditions.

Figure 12 shows that the decomposition view of first dry section 217, it comprises second sharp cooling roll 218, second driven roller 216, the first drying table 1200A, the second drying table 1101A, the 3rd drying table 1102A and the 4th drying table 1103A.In one embodiment of the invention, the first drying table 1200A, the second drying table 1101A, the 3rd drying table 1102A and the 4th drying table 1103A bloat about 140 ℃ hot-air, but the description those skilled in the art by here can understand and can change these conditions.

Figure 13 shows that the decomposition view of second dry section 224, it comprises the 3rd driven roller 223, the first drying table 1200B, the second drying table 1101B, the 3rd drying table 1102B and the 4th drying table 1103B.In one embodiment of the invention, the first drying table 1200B, the second drying table 1101B, the 3rd drying table 1102B and the 4th drying table 1103B bloat about 140 ℃ hot-air, but the description those skilled in the art by here can understand and can change these conditions.

Figure 14 is the decomposition view of the 3rd dry section 230, and it comprises 4 wheel driven action roller 229, the first drying table 1200C and the second drying table 1101C.In one embodiment of the invention, the first drying table 1200C and second drying table, 1101 C bloat about 50 ℃ hot-air, but the description those skilled in the art by here can understand and can change these conditions.

Figure 15 is the decomposition view of the 4th dry section 236, and it comprises the 5th driven roller 235, the first drying table 1200D and the second drying table 1101D.The first drying table 1200D and the second drying table 1101D bloat about 50 ℃ hot-air in one embodiment of the invention, but the description those skilled in the art by here can understand and can change these conditions.

Figure 16 shows that the decomposition view of first cleaning unit 204, it comprises gluing roller 1600, blue polymer roller 1601.In one embodiment of the invention, blue polymer roller 1601 contacts with bottom side with the top of substrate 242, and particle/foreign matter is sent on the gluing roller 1600.

Figure 17 A is depicted as the view of insulation material layer of carbon-coating printing of the embodiment of a correct registration of the present invention to 17D.Notice that Figure 17 A represents the top left side of sensor board 2106, Figure 17 B represents top right side, and Figure 17 C represents bottom left, and Figure 17 D represents the right side, bottom.Mark does not illustrate on the sensor board shown in Figure 21 A.In one embodiment of the invention, carbon printing station 103 printing is included in the carbon-coating of the solid carbon rectangle 1700 that is surrounded by rectangle carbon boundary line 1703 on the substrate 242.In follow-up print cycle, insulating materials printing station 104 prints rectangle insulation boundary line 1701 on substrate 242, and it is between solid carbon rectangle 1700 and rectangle carbon boundary line 1703.When whole four angles of being registered in of insulation material layer and carbon-coating when all being correct, generally between rectangle insulation boundary line 1701 and solid carbon rectangle 1700, there is not uncoated substrate 242.The registration of insulation material layer and carbon-coating can perhaps can use second imageing sensor 222 to check by operator's manual examination (check), in one embodiment, comprises a camera that points to each angle of substrate.Usually this has formed a part initial when printing operation begins.The operator can see whole four angles that substrate is contiguous mutually on TV (TV) screen.The operator subsequently can visual inspection in this initial procedure (in fact Remaining Stages in printing operation) insulation material layer to the registration of carbon-coating, and the adjustment of any necessity of can send as an envoy to insulation material layer and carbon-coating trace registration.Should understand web plate viewer 222 (comprising) and watch and transmit the snapshot at four angles of each card such as 4 cameras that point to position on four angles of substrate card.The angle of each card can only be observed part second on display thus, because the substrate that is positioned at when web plate passes equipment below the observation camera is replaced frequently.Any effect that insulation material layer is obtained in the registration of carbon-coating that is adjusted at that this system can make that the operator sees immediately that he taked.The adjustment that the operator takes includes, but not limited to the pulling force of serigraphy stroke, snap-on height, squeegee, and screen cloth is with respect to the position of " Y " direction, the screen cloth position with respect to " θ ".Set up the observer registration in case on this and other printing station, (use observer 228,234), can adopt automatic inner X registration arrangement (usage flag 2107,2108) and automatically the Y registration arrangement (such as, be arranged in the registration arrangement of 237A, 237B and 237C position usage flag 2101 to 2104) at the printing process monitoring and the registration of modified chi and Y method automatically.Mark 1700 to 1703 as shown in Figure 17 A to 20D can be used for the registration of automatic X and Y direction in printing process, as the optional mode of another kind or in addition, those skilled in the art can also usage flag 2101 to 2104 and 2107 and 2108 according to the record in the specification.

Figure 18 shows in the one embodiment of the invention view of insulation material layer and the incorrect registration of carbon-coating when the insulating materials layer pattern is longer than carbon-coating pattern on print direction.Even carbon has identical size with the insulating materials screen cloth on this yardstick, also can this thing happens, this be because substrate may be stretched or screen cloth the stroke of different phase (slow relatively screen cloth stroke can obtain along the longer pattern footprint of substrate screen cloth traffic direction) inequality.Notice that Figure 18 A is the top left side of sensor board 2106, the top right side that Figure 18 B is sensor board 2106, the bottom left that Figure 18 C is sensor board 2106, the right side, bottom that Figure 18 D is sensor board 2106.Go up when incorrect can be between the carbon rectangle 1700 of the insulation boundary line 1701 of rectangle and solid observed come out when one in four angles that are registered in unlapped substrate of insulation material layer and carbon-coating.The operator uses second imageing sensor 222 can check the registration of insulation material layer and carbon-coating by hand.

Figure 19 show in the one embodiment of the invention when printing insulating materials pattern than carbon printed layers shorter (such as, what be used for that the screen cloth stroke of insulating materials printing may be than carbon is longer, and what perhaps the insulating materials screen cloth may be than carbon printing station is shorter) time insulation material layer and the incorrect registration of carbon-coating view.Figure 19 A is the top left side of sensor board 2106, the top right side that Figure 19 B is sensor board 2106, the bottom left that Figure 19 C is sensor board 2106, the right side, bottom that Figure 19 D is sensor board 2106.Go up when incorrect can be between the carbon rectangle 1700 of the insulation boundary line 1701 of rectangle and solid observed come out when one in four angles of the unlapped substrate 242 of being registered in of insulation material layer and carbon-coating.The operator uses second imageing sensor 222 can check the registration of insulation material layer and carbon-coating by hand.Figure 20 A to Figure 20 D is the schematic diagram (seeing Figure 21 A) that shows the result of the printing second view guide 2002 processes, comprising the carbon rectangle 1703 of the insulating rectangular boundary line 1701 of solid carbon rectangle 1700, hollow, hollow, from the solid rectangular of the first enzyme layer 2000, from the solid rectangular and the unlapped substrate 242 of the second enzyme layer 2001.Selectable, by such as check system 237 grades of (after the second enzyme printing) in the part 6 carry out the inspection system automatically, can use such printing process aborning.Ongoing registration is generally implemented on " Y " direction by the registration arrangement (not shown) at position 237A, 237B and 237C place, and sees that by the registration control system mark 2105 (seeing Figure 21 A) implements on " X " direction.

Figure 21 A is the example of sensor board, has first view guiding, 2100 and second view guiding 2002; The one Y register mark 2101, the two Y register marks, 2102, the three Y register marks 2103 and the 4th Y register mark 2104; And X register mark 2105.Notice that X register mark 2105 comprises carbon X register mark 2107 and insulating materials X register mark 2108.Figure 21 B is the decomposition view with the delegation in the sensor board 2106 of carbon X register mark 2107 and second view guiding 2002.Figure 21 C is the decomposition view with the delegation in the sensor board 2106 of insulating materials X register mark 2108 and second view guiding 2002.Shown in Figure 21 C, insulating materials X register mark 2108 has covered carbon X register mark 2107 fully, therefore provides trigger point (left-hand side of said mark 2108) before being made in original carbon markings 2107 like this.This means that any layer subsequently will print with respect to second printed layers (being insulation material layer in this example) rather than with respect to carbon-coating.If second and sieve plate pattern subsequently longer along the size of directions X in the size of directions X (along web plate) than the first sieve plate pattern, this will be useful.

Figure 20 A-D is the decomposition view of one jiao of printing guiding, arranges according to the order that they are printed.In the part 3 of carbon printing station 103, solid carbon rectangle 1700 prints together with the rectangle carbon boundary line 1703 around this solid carbon rectangle 1700.In the part 4 of insulating materials printing station 104, printing rectangle insulation boundary line 1701 between solid carbon rectangle 1700 and rectangle carbon boundary line 1703.If insulation material layer and carbon-coating be registered on whole 4 angles all to be correct, between solid carbon rectangle 1700 and rectangle insulating materials boundary line 1701, generally can not show unlapped substrate 242 so.In addition, in the part 4 of insulating materials printing station, there are two extra rectangle insulating materials boundary lines 1701 that directly are printed on the solid carbon-coating rectangle 1700.These two superinsulation material boundary lines are in order to visually to assess the registration of the first enzyme layer 2000 and insulation material layer and the second enzyme layer 2001 and insulation material layer, shown in Figure 20 C and 20D, this can realize by the solid rectangular of printing enzyme China ink in rectangle insulating materials boundary line.Thus, third and fourth printed layers can rather than be carried out registration with respect to first printed layers with respect to second.The advantage of doing like this be pattern dimension between the ground floor and the second layer variation (this may be after first printing station such as because the heat and the pulling force that in first dry section 217, are subjected to, substrate is stretched required) can be conditioned and can not have a negative impact (generally the error at directions X is 300 μ m) the printing registration.

As illustrated in fig. 1 and 2, in the ending stage of described procedure, the substrate 242 that is printed with sensor is thereon recoiled by rewinder unit 107, and being admitted to then can be stamping machine 108 such as the Preco stamping machine that is arranged in low-humidity environment.The Preco stamping machine is CCD X, Y, and Theta, the Bolster stamping machine floats.Preco stamping machine registration arrangement uses the CCD vision system to check " the Preco point " that prints on the carbon-coating printing station, and this can make stamping machine adapt to the carbon printing and make stamping machine " punching press " go out square card.Stamping machine 108 is output as one group of punching press card shown in Figure 21 A.The punching press card is ejected into from stamping machine on the transport tape, and this transport tape is transmission card under bar code reader, and two bar codes that bar code reader can read on each card are still discarded as defective work to differentiate whether this card receives with respect to the web plate database.Can be automatically or manually extract discarded card out.These cards are deposited in top of each other then to prepare next production stage.

All be useful on the method for visual at once inspection registration after the printing process step at carbon printing station 103, insulating materials printing station 104, the first enzyme printing station 105 and the second enzyme printing station 106, it uses first imageing sensor 215, second imageing sensor 222, the 3rd imageing sensor 228, the 4th imageing sensor 234 respectively.Each part-part 3,4,5 and 6-for the screen printing manufacture process have the web plate observer camera arrangement of locating at once after the printing process step.Fig. 2 A-2C is seen in the position of web plate observer.Two cameras are arranged and have 4 cameras respectively in part 3 in part 4,5 and 6.Web plate observer camera is the part of the artificial setting up procedure that used by web plate machine operation person between elementary period at print run.Camera is used to observe typographic(al) mark, and it helps the initial setting of carbon alignment substrate 242 and the registration of insulating materials course carbon-coating, the first enzyme course insulation material layer and the second enzyme course insulation material layer.The printing guiding is shown in Figure 21 A.For the carbon print register, the second observation guiding 2100 be used to indicate running during through carbon printing station 103 the carbon printing with respect to the position at substrate 242 edges.Leading line and draught line have been shown in Figure 21 A.The carbon trace is adjusted, and meets at right angles up to above-mentioned line indication trace and substrate edge.See Figure 21 A, need carry out registration to independent printed layers at directions X (along the length of machine) and Y direction (passing the width of machine).The directions X registration is controlled by the inside registration arrangement of machine.It utilizes the zone that is printed shown in Figure 21 A, B and the C.In the carbon print cycle, a carbon X register mark 2107 is printed in this zone.In order to print dielectric ink in correct position, the insulating materials print cycle utilizes sensor to be registrated on the carbon trace, and it uses carbon X register mark 2107 to regulate insulated screens.The carbon X register mark 2107 that is used for this purpose then covers printing with the insulating materials printing with insulation X register mark 2108, and is used in the same way with correctly the registration first enzyme layer 2000 and the second enzyme layer 2001.Y direction registration is positioned at the Y registration arrangement (not shown) at position 237A, 237B and 237C place to be controlled, and in one embodiment of the invention, it can be that a model of buying from German Leopoldshoehe is the Eltromat registration arrangement of DGC 650.It utilizes the printing zone 2101 to 2104 shown in Figure 21 A.In each print cycle-carbon, insulating materials, enzyme 1 and these marks of enzyme 2-are printed so that follow-up printing via sensor at Y direction registration.Web plate data-base recording procedural information during printing.The information that writes down in database can find root through a bar code in each independent card, use the 2D bar code in one embodiment.The typical information of collecting in the web plate database is listed in the table 3.Whether the web plate database has procedure parameter of assessment can accept the function that maybe can not receive, and can be used for refusing some card-parameter on this basis whether in their tolerance limit.Unacceptable card can manually or automatically be removed in the process below.

Table 3

Figure 22 is the schematic diagram of the parameter X, Y, Z and the θ that are used for registration screen printing process.The direction of the parameter Y representative machine side from operator to the screen printer (being generally level).Parameter X representative direction (being generally level) from unwinding unit 101 to rewinder unit 107.Parameter Z representative and the X direction (be generally vertical) vertical with the Y direction.Parameter θ representative is around the angle of Z axle.In one embodiment of the invention, following parameter is used to the following printing process of registration, for example carbon printing station 103, insulating materials printing station 104, the first enzyme printing station 105 and the second enzyme printing station 106.

In one embodiment of the invention, the output of web plate production process is to be printed on the card that comprises carbon, insulating materials and two same enzyme layer patterns, and described pattern registration ground printing each other constitutes the bar of an electrochemical sensor that comprises the glucose that is used for detecting blood sample respectively and the contact electrode that is associated.This is used for carrying out the monitoring certainly of blood-glucose with the quantifier synergy.Imagined the multiple deisgn product of bar.Present this web plate is designed to produce " the One Touch Ultra " bar that can be used on from the One Touch Ultra quantifier that LifeScan company buys.

It in Figure 21 A the schematic diagram of the sample of the pattern produced.It is depicted as the card that a quilt prints fully, and it comprises 10 " OK " 50 " bar ".Every card has 500 " bars " altogether.Wherein also show printing direction-orienting.By printing 0 to 9 row (each 50) parallel with print direction, described process can easily extend to Jiang Gehang cutting step separated from one another.And this method, can easily be identified in any defective row that forms by the variation of web plate in the printing quality (perpendicular to print direction).Every row is assigned a numeral (by bar-code identification), can discern and the special row of eliminating on plate special on the web plate in the comparable data storehouse subsequently thus, and does not need refusal to abandon whole plate.Improved the output of enabled production in the process like this and made the efficient of whole process higher.

Movably flat substantially screen cloth adapts to the type (liquid/solid composition) that is used in the China ink in the electrochemical sensor printing well.Use flat mesh movably can control the deposition of the thick-layer of the required China ink of print definition and electrochemical sensor better than rotogravure printing or cylinder serigraphy.The screen cloth of multiple screen type (diameter, line interval, thickness, web plate lattice number with different web plate lattice, web plate ruling) can be in commercial acquisition to satisfy the different needs of dissimilar China inks in continuous screen printing process (carbon, insulating materials, enzyme).

Because flat mesh, print roller, substrate and force the setting of screen cloth, can obtain various operating parameters (position of screen cloth to the angle of substrate, squeegee angle, screen cloth to squeegee, position that squeegee arrives print roller, snap-on are apart from the relative speed of, substrate and screen cloth and squeegee) so that the printing process optimization of electrochemical sensor towards the squeegee of substrate.

Brief overview is produced the web plate production process of electrochemical sensor, can expand or stretch when web plate is heated and is in pulling force following time during the course.Printing station (such as carbon, insulating materials, two enzyme printing stations) is generally all closelyed follow drying table.For dry black effectively, drying table is in quite high temperature (50-140 degree centigrade) operation down.In addition, pass through the registration of each printing station in order to help web plate, web plate places under the pulling force.

Substrate must remain under the pulling force with the control registration in this process, and the result is as long as substrate is heated, and such as the dry China ink in printing back, substrate all causes the variation of picture size in printing subsequently be stretched unpredictablely.

Size of images is by multiple factor (relative velocity of the viscosity of template size, China ink, relevant web plate and sieve plate/screen cloth and substrate are at that elongation (reversible with irreversible stretching) etc.) decision in each printing station.When finishing, process can find the change (between different print steps) of picture size.Unpredictable higher than the remarkable reduction of expected yield.Do not surpass 300 μ m if interlayer picture size matches along web plate (directions X) direction, product will lose efficacy.Excessive picture size changes and is considered to because the too much contraction with uncertain stretching (because heating and pulling force) and web plate substrate causes.

The problem that stretching and pulling force bring can not cause problem same in planographic.In order to solve the problem in the screen printing process, attempt using pre-shrunk substrate.This substrate was heated to about 185 degrees centigrade before being used to the screen printing process.Yet the change of picture size still is a problem and causes output to reduce.

Suggestion for this screen printing process is to use high temperature or more suitably carry out preliminary treatment in first drier under sufficiently high temperature at present, so that in one embodiment, before being printed onto on the substrate, get rid of the irreversible stretching of substrate substantially to image.

In first treating stations of web plate machine, a drying table is heated to substrate up to 160 degrees centigrade.The temperature that substrate is subjected in subsequent process generally is no more than 140 degree.

In Fig. 2 A, first warm table that the substrate that is not printed runs into is a hot plate.It is the plate that applies Teflon, raises and contact substrate in the web plate motion process.Heat is introduced in the back side of substrate.It turns round in 160 ℃+/-4 ℃ specifications of setting value usually.160 ℃ setting value provides best size Control satisfactorily.The mean value that calculates is 160.9 ℃.At platform 2 places, be incorporated into the front surface of substrate at the Hot air quilt that sets value 160 ℃+/-4 ℃ of specifications.The mean value that calculates is 161.29 ℃.At platform 3 places, the Hot air quilt that sets value 160 ℃+/-4 ℃ of specifications is incorporated into the front surface of substrate.The mean value that calculates is 161.18 ℃.At platform 4 places, the Hot air quilt that sets value 160 ℃+/-4 ℃ of specifications is incorporated into the front surface of substrate.The mean value that calculates is 160.70 ℃.

The result that the heat of introducing in web plate pulling force and the drier brings is that each pattern of web plate substrate repeats to be stretched about about 0.7mm.This is to utilize the main cause of stablizing substrate as the platform 1 of pretreatment unit before follow-up printing station.Use 1 pair of substrate of platform to carry out the stability that preliminary treatment has improved carbon and insulating materials line length, because many stretchings of material are got rid of from substrate before printing.

Among the embodiment according to lower screen humidifying of the present invention, the cylinder of a hollow is positioned at the bottom of an enzyme printing mesh screen, just in the front of print roller.Article one, pipeline takes moisture in the hollow cylinder to.When the print roller of a vicinity moved, cylinder moved with it, the whole bottom of humidifying screen cloth.The character that the enzyme humidifying can be used for guaranteeing the enzyme China ink run through print between on-stream period overflow with printing process in do not change especially their viscosity (thickness) and water content.

In one embodiment of the invention, relative humidity in cover is between 85 to 95%, temperature in the cover is between 18 to 22 ℃, and the outer envionmental humidity of cover is approximately-50+/-10%, covers approximately-20 ℃+/-2 ℃ of outer temperature, wish that the interior relative humidity of cover remains on 87.69% mean value, Cp is 2.81, and Cpk is 1.52, and wishes that the temperature in the cover remains on 20.59 ℃ mean value, Cp is 5.60, and Cpk is 3.94.

Be understandable that equivalent structure can substitute here shown in and described structure, the embodiment of the invention of having described is not only claimed structure of the present invention of implementing.In addition, be understandable that every kind of above-mentioned structure has certain function, these structures can be used as the reference of the mode of implementing this function.

Though illustrated and described the preferred embodiments of the present invention here, it is apparent that to those skilled in the art these embodiment only are that mode with example provides.Those skilled in the art can expect multiple change, variation and alternative, and inconvenient from the present invention.Be understood that when enforcement is of the present invention and can carry out various replacements the embodiment of the invention described here.Following claim defines scope of the present invention, and method and structure and equivalent way thereof in these claim scopes are covered by it.

Claims

1. method that in the web plate production process, improves printing quality, wherein said web plate production process comprises that at least one is suitable for enzyme is printed onto mobile on-chip printing station, described web plate production process may further comprise the steps:

Move described substrate continuously by described process;

By the screen printing process enzyme China ink is deposited on the described substrate, its China and Mexico are deposited on the end face of described screen cloth, and stressed passing on the described substrate that described end face arrives and described screen cloth bottom surface is contiguous;

Humidifying is in air to the first relative humidity at described face place of described screen cloth;

Humidifying is in air to the second relative humidity at the place, described bottom surface of described screen cloth.

2. the method that improves printing quality in the web plate production process according to claim 1, wherein said first relative humidity is between 85% to 95%.

3. the method that improves printing quality in the web plate production process according to claim 2, wherein said first relative humidity is between 87% to 88%.

4. the method that improves printing quality in the web plate production process according to claim 3, wherein said first relative humidity is 87.69%.

5. the method that improves printing quality in the web plate production process according to claim 1, wherein said second relative humidity is between 85% to 95%.

6. the method that improves printing quality in the web plate production process according to claim 5, wherein said second relative humidity is between 87% to 88%.

7. the method that improves printing quality in the web plate production process according to claim 6, wherein said second relative humidity is 87.69%.

8. method that in the web plate production process, improves printing quality, wherein said web plate production process comprises that at least one is suitable for enzyme is printed onto mobile on-chip printing station, described web plate production process may further comprise the steps:

Move described substrate continuously by described process;

The air of heating in described screen area is to first predetermined temperature; And

Described air to the first relative humidity of humidifying.

9. the method that in the web plate production process, improves printing quality according to claim 8, wherein said first relative humidity is between 85% to 95%, and described first predetermined temperature is between 18 to 22 degrees centigrade.

10. the method that in the web plate production process, improves printing quality according to claim 9, wherein said first relative humidity is between 87% to 88%, and described first predetermined temperature is between 18 degrees centigrade to 22 degrees centigrade.

11. the method that improves printing quality in the web plate production process according to claim 10, wherein said first relative humidity is 87.69%, and described first predetermined temperature is 20.59 degrees centigrade.

12. the method that improves printing quality in the web plate production process according to claim 8, wherein said substrate was cooled to second predetermined temperature before described deposition step.

13. the method that improves printing quality in the web plate production process according to claim 12, wherein said substrate was cooled to 18 degrees centigrade temperature before described deposition step.