CN2195757Y - Disposable biosensor - Google Patents
Disposable biosensor Download PDFInfo
- Publication number
- CN2195757Y CN2195757Y CN 94212913 CN94212913U CN2195757Y CN 2195757 Y CN2195757 Y CN 2195757Y CN 94212913 CN94212913 CN 94212913 CN 94212913 U CN94212913 U CN 94212913U CN 2195757 Y CN2195757 Y CN 2195757Y
- Authority
- CN
- China
- Prior art keywords
- electrode
- film
- sensor
- biology sensor
- coating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229910052751 metal Inorganic materials 0.000 claims abstract description 7
- 239000002184 metal Substances 0.000 claims abstract description 7
- 239000000758 substrate Substances 0.000 claims abstract description 7
- 239000012528 membrane Substances 0.000 claims description 11
- 230000003628 erosive effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 150000002500 ions Chemical class 0.000 abstract description 3
- 238000007639 printing Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 2
- 239000011248 coating agent Substances 0.000 abstract 2
- 238000000576 coating method Methods 0.000 abstract 2
- 108010093096 Immobilized Enzymes Proteins 0.000 abstract 1
- 239000000463 material Substances 0.000 description 12
- 238000005259 measurement Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 238000007385 chemical modification Methods 0.000 description 4
- 238000012544 monitoring process Methods 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229910021607 Silver chloride Inorganic materials 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003968 anodic stripping voltammetry Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 150000004054 benzoquinones Chemical class 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- KTWOOEGAPBSYNW-UHFFFAOYSA-N ferrocene Chemical compound [Fe+2].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 KTWOOEGAPBSYNW-UHFFFAOYSA-N 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000001235 sensitizing effect Effects 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000004528 spin coating Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model belongs to a disposable biosensor capable of measuring the content of a substance in solution. The biosensor is formed by coating a conducting layer and an insulating protection ring on the surface of a sheet metal serving as a conducting substrate by a printing process to form a basic electrode, and coating an electron acceptor film, an immobilized enzyme film and an anti-interference film on the surface of the basic electrode. The base electrode can also be used as an electrochemical detector for determining the concentration of heavy metal ions.
Description
The utility model belongs to the biology sensor that can measure a kind of content of material in the solution.This biology sensor can in aqueous solution, detect a kind of material existence, measure a kind of concentration of material and monitor the variation of its concentration within a certain period of time.
Can measure that a kind of biology sensor of content of material has polytype in the solution, as metal electrode biology sensor based on current measurement, based on the ion-sensitive field effect transistor biology sensor of potential measurement, optical fiber biosensor and thermistor biology sensor etc.Although the biology sensor of numerous types obtains development at home and abroad,, thereby be difficult to realize commercialization because the manufacturing process that is adopted is not suitable for mass, automated production.Document Bioseneors, 3,317-318(1998) with Sensors and Actuators B, 8,59-64(1992) reported a kind of biology sensor that reaches and be suitable for commercialization production.The profile of this biology sensor is the rectangle thin slice, on polyimide substrate, at first stamp two bullion buss with screen printing mode, stamp the graphite layer on one therein, apply the immobilized glucose oxidase film that contains electronics biography acceptor again, be used to detect glucose; On another silver bar, stamp silver chloride layer, again with encapsulating material sealing sheet surface, expose electrode and the silver chloride electrode that is attached with fixed enzyme membrane at thin slice one end, become the sensitive part of measuring samples, expose a part of electrode, thin slice and measuring instrument are joined at the thin slice other end.At last, on sensitive part, post nylon wire, play the protection fixed enzyme membrane.In the structure of this biology sensor, because reference electrode and working electrode are to do on a substrate, use back and working electrode together discarded, thereby improved analysis cost, cause waste.In addition, comprised the technological process of making reference electrode in the manufacturing process, so the manufacturing process complexity.Because this biology sensor belongs to planar structure, need a segment distance by the sensitizing range to contact jaw and do transition, thereby volume is difficult to dwindle, and difficultly realize integral packaging, to carrying and storage is made troubles.
The purpose of this utility model provide a kind of on the sheet metal substrate with printing process coated with conductive layer successively, protection ring, chemical modification layer, immobilized biological activated film, and interference rejection membrane and constitute the disposable biological sensor of sheet.
When biology sensor contacts with detected solution, the molecule of measured matter will pass interference rejection membrane and arrive immobilized biological activated film, and big molecule or have different electrical material molecules with measured matter and will be blocked.The molecule of measured object is in bioactivator (as: enzyme) oxidation of oxidation state in immobilized biological activated film, bioactivator is converted into and goes back ortho states simultaneously, and then being reverted to oxidation state by the electron mediator oxidation of lower floor, electron mediator becomes simultaneously goes back ortho states.Also the electron mediator of ortho states discharges electronics at the conductive layer surface that is under the certain potentials, and reverts to oxidation state, and above process circulation is carried out.The electronics that is discharged by electron mediator causes the variation of measuring electric current, and passes to metering circuit by metal substrate, can be reflected the concentration of measured matter by the current change quantity of metering circuit detection.
The utility model is compared with present existing biology sensor, owing to adopted the sheet conductive structure, thereby have littler area and volume, thus saved the volume of manufactured materials and analytic sample.Simultaneously, owing to adopted discrete reference electrode, thus simplified manufacturing process, also avoided waste.Requirement to sample during measurement is very little, from several microlitres to several milliliters, but operate as normal all.In addition, because the volume of this sensor is very little, shape such as tablet are suitable for the mechanization integral packaging, are easy to carry, store and transport.
This Design of Sensor scheme accompanying drawings is as follows.
Accompanying drawing 1 is the structural representation of biology sensor.
At accompanying drawing 1(a) in, by metal substrate 1, printed conductive layer 2, protection ring 3 formation base electrodes.Conductive layer can be the tackifier that is mixed with the superfine graphite powder, also can be the tackifier of other inert metal powder, and its effect is to generate to have electrochemical stability, and the film of conduction is as the working electrode of sensor.Protection ring can be that silicon rubber, printing ink etc. are water-fast, the material of acid-alkali-corrosive-resisting, and its effect is to limit the physical dimension that the centre as the conductive layer of working electrode contacts with solution, and the remainder on isolation sensor surface, prevents the etch of aqueous solution.This basic electrode can be used for detecting the material with electrochemical activity.Form chemical modification layer 4, immobilized biological activated film 5 and interference rejection membrane 6 on the basic electrode surface successively with the method for spin coating or dropping.The chemical modification layer is made up of electron mediator, and as ferrocene and derivant thereof, benzoquinones, organic dyestuff etc., its effect is the electrochemical redox process on catalysis electrode surface, reduces measuring voltage, lifts from detection sensitivity.Immobilized biological activated film can be fixed enzyme membrane, immobilized microorganism film, immobilization biological tissue film and immobilized antigen antibody membrane etc., its effect is the tested organic substance of catalysis, the product with electrochemical activity that its generation can be detected by basic electrode.When measuring different material, can select corresponding immobilized biological activated film for use.Interference rejection membrane can be cellulose membrane, ion exchange resin etc., and its effect is to stop that macromolecular substances arrives electrode surface, avoids doing resisting and preventing and pollutes.The profile of the biology sensor after making finishes is as accompanying drawing 1(b) shown in 7.
During measurement, biology sensor and reusable reference electrode constitute measures the loop, and the electric signal of sensor output shows the concentration of a kind of material in the solution after metering circuit is handled.As the measurement to glucose, linear measurement range is 0.5-25mmol/L, reaches 55mmol/L from detectability, and the response time is 25 seconds, and each Measuring Time is 30-45 second.Biology sensor after encapsulation is preserved down at 4 ℃, and the term of validity 8 was to 12 months.Use basic electrode, with the concentration of lead ion in the anodic stripping voltammetry measurement solution, linear measurement range reaches 0.05-100 μ g/L.
The utility model can carry out chemical modification on the basic electrode surface, constitute chemically modified electrode, also can apply various fixed enzyme membrane, immobilized microorganism film, biological tissue's fixed film, thereby formation enzyme biologic sensor, microbiological sensor and bio-Sensor Based on Tissue realize the measurement to multiple material.Basic electrode can be used as electrochemical detector, is used to measure the concentration of heavy metal ion.
This biology sensor is applicable to fields such as clinical examination, domestic monitoring, environmental monitoring, health examination, labour protection, Monitoring of Chemical, sweat control.
Claims (1)
1, a kind of disposable biological sensor is characterized in that printed conductive layer, protection ring, chemistry are repaiied erosion layer, immobilized biological activated film and interference rejection membrane on the sheet metal substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94212913 CN2195757Y (en) | 1994-05-26 | 1994-05-26 | Disposable biosensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94212913 CN2195757Y (en) | 1994-05-26 | 1994-05-26 | Disposable biosensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2195757Y true CN2195757Y (en) | 1995-04-26 |
Family
ID=33829622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94212913 Expired - Fee Related CN2195757Y (en) | 1994-05-26 | 1994-05-26 | Disposable biosensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2195757Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100422723C (en) * | 2006-03-14 | 2008-10-01 | 浙江大学 | Metal ion inspection based on low-voltage liquid electrode plasma |
| CN100465634C (en) * | 2002-10-30 | 2009-03-04 | 因弗内斯医疗有限公司 | Preconditioning of a substrate in a continuous process for manufacture of electrochemical sensors |
-
1994
- 1994-05-26 CN CN 94212913 patent/CN2195757Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100465634C (en) * | 2002-10-30 | 2009-03-04 | 因弗内斯医疗有限公司 | Preconditioning of a substrate in a continuous process for manufacture of electrochemical sensors |
| CN100489513C (en) * | 2002-10-30 | 2009-05-20 | 因弗内斯医疗有限公司 | Method of manufacture of electrochemical sensors |
| CN1732382B (en) * | 2002-10-30 | 2011-01-12 | 因弗内斯医疗有限公司 | Manufacture of electrochemical sensors by moveable flat screen printing |
| CN100422723C (en) * | 2006-03-14 | 2008-10-01 | 浙江大学 | Metal ion inspection based on low-voltage liquid electrode plasma |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |