CN100368072C - Visualized aquogel, and its preparing method - Google Patents
Visualized aquogel, and its preparing method Download PDFInfo
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- CN100368072C CN100368072C CNB2005100944004A CN200510094400A CN100368072C CN 100368072 C CN100368072 C CN 100368072C CN B2005100944004 A CNB2005100944004 A CN B2005100944004A CN 200510094400 A CN200510094400 A CN 200510094400A CN 100368072 C CN100368072 C CN 100368072C
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Abstract
The present invention provides visualized hydrogel and a preparing method thereof, which relates to hydrogel and a preparing method thereof. The hydrogel is composed of one layer of sensitive hydrogel and one layer of non sensitive hydrogel adhesives. The present invention has the preparing method: monomers of the hydrogel, crosslinking agents, sensitive elements and initiating agents are dissolved in solvents according to the proportion of 100 to 10: 1 to 0.1: 10 to 1: 1 to 0.1 until the weight-to-volume ratio is from 4% to 30%, and the sensitive aquagel is formed by the polymerization; the monomers of the hydrogel, the crosslinking agents and the initiating agents are dissolved in water according to the proportion of 100 to 10: 1 to 0.1: 10 to 0.1 until the weight-to-volume ratio is from 4% to 30%, and the non-sensitive hydrogel is formed by the polymerization; the sensitive hydrogel obtained by the step 1) and the non-sensitive hydrogel obtained by the step 2) are bonded together to form visualized hydrogel. The present invention can be used for analyzing metallic ions, nucleic acids, proteins, etc., and the analyzed results can be observed not by using any instrument.
Description
Technical field
The present invention relates to a kind of visual double-deck hydrogel and preparation method thereof.Utilize sensitive aquagel response external environment and produce deformation.Specifically, the present invention relates in hydrogel fixedly that sensitive molecule (group) forms sensitive aquagel, again sensitive aquagel and non-sensitive hydrogel are bonded together, promptly get a kind of visualized aquogel.The technical field that belongs to visual analyzing.
Background technology
Hydrogel is the hydrophilic high molecular polymer of a class, it has certain biocompatibility, has the expansion that causes because of osmotic pressure and the character of contraction, also has memory function, properties such as environment pattern before can replica polymerization, therefore hydrogel has been applied to application studies such as tissue repair, medicament slow release, template imprinting.
Because hydrogel can response environment variation and be applied to sensing Study of An, PAHG for example, under alkali condition, amide groups is hydrolyzed into carboxyl group--can induced environment in pH be worth changing and the variation of volume take place, in hydrogel fixing on behind specific molecular (antibody etc.) and its corresponding aglucon (antigen etc.), hydrogel can respond the environment that contains this specific molecular or corresponding aglucon, a little less than the Volume Changes naked eyes resolution capability of these responses.With forming crystal colloid array gel behind the neutral water gel locking crystal colloid array, in crystal colloid array gel, fix specific molecule, be intelligent crystal colloid array gel.The Bragg peak value that its deformation that induction of environment is caused can show the crystal colloid that locks in the gel moves.The color that perusal shows as intelligent crystal colloid array gel changes, but the variation naked eyes of Bragg peak value in certain wave-length coverage can not be distinguished.For example in the 600-700nm scope, change, all show as redness.
Summary of the invention
Technical problem: the purpose of this invention is to provide visual double-deck hydrogel that a kind of naked eyes of quick deformation can distinguish and preparation method thereof.
Technical scheme: the present invention adopts following technical scheme:
Visual double-deck hydrogel of the present invention is about to sensitive aquagel and non-sensitive hydrogel and is bonded together the visual double-deck hydrogel of the deformation of formation.
The method of the visual double-deck hydrogel of preparation of the present invention, that is:
1. with monomer, crosslinking agent, sensing element, the initator of hydrogel, in 100～10: 1～0.1: 10～1: 1～0.1 ratio be dissolved in the solvent to w/v be 4～30%, polymerization forms sensitive aquagel,
2. with the monomer of hydrogel, crosslinking agent, initator, in 100～10: 1～0.1: 10～0.1 ratio soluble in water to w/v be 4～30%, polymerization forms non-sensitive hydrogel,
3. the non-sensitive hydrogel that obtains of sensitive aquagel that above-mentioned steps 1 is obtained and step 2 is bonded together, and promptly forms visual double-deck hydrogel.
Described hydrogel monomer can be acrylamide or amino substituted acrylamide,
Described crosslinking agent can be a methylene diacrylamide,
Described sensing element can be the molecule of chemical synthesis such as the molecule that identification can take place with other molecule for 4-vinyl benzene-18-hat-6,4-propenylbenzene-18-hat-6 and 3-aminobenzene boric acid etc., also can be natural biomolecule such as antibody, nucleic acid strand and enzyme
Described solvent is a water.
Beneficial effect: compared with prior art, the present invention has following advantage:
1. visualized aquogel of the present invention and intelligent crystal colloid array gel phase ratio, fixedly the condition of sensing element is not harsh, can former polymerization fix, and can fix after the polymerization yet again, and can directly fixedly resemble the large biological molecule of this class of antigen antibody complex.
2. visualized aquogel of the present invention and intelligent crystal colloid array gel phase ratio, polymerization single polymerization monomer can be neutral monomers, also can not be neutral monomer, and intelligent crystal colloid array gel intelligence can only be neutral polymerization single polymerization monomers.
3. the great advantage of visualized aquogel of the present invention is that the deformation that environmental response is produced is the variation that bends, and can observe intuitively by naked eyes, and is highly sensitive.
The hydrogel of visual analyzing of the present invention, the hydrogel of prepared visual analyzing can detect metal ion, nucleic acid and albumen etc.
The specific embodiment
Embodiment 1: the preparation method who is used to prepare the visualized aquogel that detects lead ion of the present invention follows these steps to carry out successively:
1) with 4-vinyl benzene-18-hat-6 or 4-propenylbenzene-18-hat-6, acrylamide, methene acrylamide (mol ratio is 1: 20: 1)) to be dissolved in w/v be in 1.5: 10 water, to be added to (clamp bar thickness is 160 microns) polymerization film formation in the mould.
2) with acrylamide: it is in 1.5: 10 water, to be added to (clamp bar thickness is 160 microns) polymerization film formation in the mould that methene acrylamide (mol ratio is 30: 1) is dissolved in w/v.
3) above-mentioned two kinds of glued membranes are placed dry dehydration 80% on the glass plate, form mould with 320 microns clamp bar again, add step 2) described acrylamide and the polymerization of methene acrylamide solution, promptly form the visualized aquogel that detects lead ion.
Embodiment 2: the preparation method who is used to prepare the visualized aquogel that detects hepatitis B surface antigen of the present invention follows these steps to carry out successively:
1) acrylamide: methene acrylamide (mol ratio is 30: 1)) being dissolved in w/v is in 3: 10 water, to be added to (clamp bar thickness is 160 microns) polymerization film formation in the mould;
2) above-mentioned glued membrane places dry dehydration 90% on the glass plate, and the clamp bar that adds a flat glass plate thickness again and be 320 microns is formed mould;
3) activate acrylic acid with active ester method, form the acrylic acid of activation;
4) modify hepatitis B surface antigen with the acrylic acid of activation and form the acrylic hepatitis B surface antigen, modify the antibody of the antibody formation acrylic anti-hepatitis B surface antigen of anti-hepatitis B surface antigen with the acrylic acid of activation, (mol ratio is 1: 1: 20: 1) be dissolved in w/v and be in 3: 10 water for the antibody of acrylic hepatitis B surface antigen, acrylic anti-hepatitis B surface antigen, acrylamide and methene acrylamide, be added to 2 again) polymerization film formation in the mould, promptly form the visualized aquogel that detects hepatitis B surface antigen.
Embodiment 3: the preparation method who is used to prepare the visualized aquogel that detects the random nucleic acid segment of the present invention follows these steps to carry out successively:
1) N-isopropylacrylamide: it is in 3: 10 water, to be added to (clamp bar thickness is 160 microns) polymerization film formation in the mould that methene acrylamide (mol ratio is 30: 1) is dissolved in w/v;
2) above-mentioned glued membrane is placed dry dehydration 90% on the glass plate, the clamp bar that adds a flat glass plate thickness again and be 320 microns is formed mould;
3) activate acrylic acid with active ester method, form the acrylic acid of activation;
4) modify amidized nucleic acid fragment with the acrylic acid of activation and form the acrylic nucleic acid fragment, modify the complementary fragment of anti-amidized nucleic acid fragment with the acrylic acid of activation and form the complementary fragment of acrylic nucleic acid fragment, (mol ratio is 3: 3: 30: 1) be dissolved in w/v and be in 3: 10 water for the complementary fragment of acrylic nucleic acid fragment, acrylic nucleic acid fragment, acrylamide and methene acrylamide, be added to 2 again) polymerization film formation in the mould, promptly form the visualized aquogel that detects the random nucleic acid segment.
Embodiment 4: the preparation method who is used to prepare the visualized aquogel that detects organophosphorus insecticide of the present invention follows these steps to carry out successively:
1) acrylamide: it is in 3: 10 water, to be added to (clamp bar thickness is 160 microns) polymerization film formation in the mould that methene acrylamide (mol ratio is 30: 1) is dissolved in w/v;
2) above-mentioned glued membrane is placed dry dehydration 90% on the glass plate, the clamp bar that adds a flat glass plate thickness again and be 320 microns is formed mould;
3) activate acrylic acid with active ester method, form the acrylic acid of activation, modify acetylcholinesterase with the acrylic acid of activation again;
4) acetylcholinesterase, acrylamide and the methene acrylamide (mol ratio is 1: 100: 4) modified of acrylic acid to be dissolved in w/v be in 1: 10 water, to be added to 2 again) polymerization film formation in the mould, promptly form the visualized aquogel that detects organophosphorus insecticide.
Claims (3)
1. visualized aquogel, it is characterized in that this hydrogel is bonded together by one deck sensitive aquagel and the non-sensitive hydrogel of one deck constitutes, and wherein the sensing element in the sensitive aquagel is 4-vinyl benzene-18-hat-6,4-propenylbenzene-18-hat-6 and 3-aminobenzene boric acid, antibody, nucleic acid strand or enzyme.
2. preparation method who is used for the described visualized aquogel of claim 1 is characterized in that the method for preparing is:
1) monomer of hydrogel, crosslinking agent, sensing element, initator are 100～10: 1～0.1 in molar ratio: 10～1: 1～0.1 ratio soluble in water to w/v be 4～30%, polymerization forms sensitive aquagel; Sensing element is that the branch period of the day from 11 p.m. to 1 a.m of chemical synthesis is: 4-vinyl benzene-18-hat-6 and 3-aminobenzene boric acid when being natural biomolecule are: enzyme, antibody and nucleic acid strand;
2) monomer of hydrogel, crosslinking agent, initator are 100～10: 1～0.1 in molar ratio: 10～0.1 ratio soluble in water to w/v be 4～30%, polymerization forms non-sensitive hydrogel;
3) with above-mentioned steps 1) sensitive aquagel and the step 2 that obtain) the non-sensitive hydrogel that obtains is bonded together, and promptly forms visual double-deck hydrogel.
3. the preparation method of visualized aquogel as claimed in claim 2, the monomer that it is characterized in that hydrogel is acrylamide or amino substituted acrylamide, and crosslinking agent is a methylene diacrylamide, and sensing element is the molecule of chemical synthesis.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100944004A CN100368072C (en) | 2005-09-16 | 2005-09-16 | Visualized aquogel, and its preparing method |
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| CNB2005100944004A CN100368072C (en) | 2005-09-16 | 2005-09-16 | Visualized aquogel, and its preparing method |
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| CN1757431A CN1757431A (en) | 2006-04-12 |
| CN100368072C true CN100368072C (en) | 2008-02-13 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101513608B (en) * | 2009-02-27 | 2010-10-27 | 东南大学 | Visualization detecting aquagel of melamine and preparing method thereof |
| CN102634043B (en) * | 2012-04-27 | 2013-06-26 | 广州吉家庄生物科技有限公司 | Supermolecular hydrogel and preparation method and application thereof |
| CN104297429A (en) * | 2014-10-15 | 2015-01-21 | 广州甘蔗糖业研究所 | Monosaccharide sensitive type hydrogel and preparation method thereof |
| CN111892689B (en) * | 2020-08-14 | 2023-04-25 | 东南大学成贤学院 | Nucleic acid hydrogel and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1118376A (en) * | 1994-09-06 | 1996-03-13 | 中国科学院长春应用化学研究所 | Organic phase biosensor prepared from low-temperature hydrogel immobilized enzyme |
| CN1631914A (en) * | 2004-11-24 | 2005-06-29 | 东南大学 | Space length adjustable crystal gel and method for preparing same |
| WO2005072334A2 (en) * | 2004-01-23 | 2005-08-11 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Photoresponsive hydrogels |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1118376A (en) * | 1994-09-06 | 1996-03-13 | 中国科学院长春应用化学研究所 | Organic phase biosensor prepared from low-temperature hydrogel immobilized enzyme |
| WO2005072334A2 (en) * | 2004-01-23 | 2005-08-11 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Photoresponsive hydrogels |
| CN1631914A (en) * | 2004-11-24 | 2005-06-29 | 东南大学 | Space length adjustable crystal gel and method for preparing same |
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Assignee: Hydron Contact Lens Co., Ltd. Assignor: Southeast University Contract fulfillment period: 2008.7.1 to 2015.6.30 contract change Contract record no.: 2009990000866 Denomination of invention: Visualized aquogel, and its preparing method Granted publication date: 20080213 License type: Exclusive license Record date: 2009.8.10 |
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