CN100366303C - Preparation and application of anti blocking biomembrane for tendon and ligament - Google Patents
Preparation and application of anti blocking biomembrane for tendon and ligament Download PDFInfo
- Publication number
- CN100366303C CN100366303C CNB2004101015975A CN200410101597A CN100366303C CN 100366303 C CN100366303 C CN 100366303C CN B2004101015975 A CNB2004101015975 A CN B2004101015975A CN 200410101597 A CN200410101597 A CN 200410101597A CN 100366303 C CN100366303 C CN 100366303C
- Authority
- CN
- China
- Prior art keywords
- tendon
- biomembrane
- ligament
- adhesion
- tendons
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000002435 tendon Anatomy 0.000 title claims abstract description 27
- 210000003041 ligament Anatomy 0.000 title claims abstract description 18
- 230000000903 blocking effect Effects 0.000 title claims description 11
- 238000002360 preparation method Methods 0.000 title claims description 6
- 239000000463 material Substances 0.000 claims abstract description 15
- 102000008186 Collagen Human genes 0.000 claims abstract description 10
- 108010035532 Collagen Proteins 0.000 claims abstract description 10
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229920001287 Chondroitin sulfate Polymers 0.000 claims abstract description 5
- 239000003513 alkali Substances 0.000 claims abstract description 5
- 239000002585 base Substances 0.000 claims abstract description 5
- 229940059329 chondroitin sulfate Drugs 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 5
- 241000283690 Bos taurus Species 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 150000004965 peroxy acids Chemical class 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 229920001436 collagen Polymers 0.000 abstract description 7
- 102000004190 Enzymes Human genes 0.000 abstract description 4
- 108090000790 Enzymes Proteins 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 239000012528 membrane Substances 0.000 abstract description 3
- 239000011148 porous material Substances 0.000 abstract description 3
- 239000002253 acid Substances 0.000 abstract description 2
- 239000012535 impurity Substances 0.000 abstract description 2
- 230000002093 peripheral effect Effects 0.000 abstract 2
- 235000015278 beef Nutrition 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 238000004804 winding Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- 102000012422 Collagen Type I Human genes 0.000 description 4
- 108010022452 Collagen Type I Proteins 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000001356 surgical procedure Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 3
- 230000002980 postoperative effect Effects 0.000 description 3
- 206010061223 Ligament injury Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 208000021945 Tendon injury Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 108010080379 Fibrin Tissue Adhesive Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000589 cicatrix Anatomy 0.000 description 1
- -1 clean Substances 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- 210000001951 dura mater Anatomy 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/043—Proteins; Polypeptides; Degradation products thereof
- A61L31/044—Collagen
Abstract
The present invention discloses a method for preparing an adhesion preventing biomembrane for tendons and ligaments, which contains spongy medical collagen material with pores, has the advantages of stable structure and favorable biocompatibility and can be degraded and absorbed, and an application of the adhesion preventing biomembrane. Tissues of beef tendons are used as base material, impurities are removed, and then, the tissues are washed, treated by acid, alkali and enzymes and crosslinked by chondroitin sulfate. I-shaped collagen with protein purity higher than 95% is lyophilized to obtain a degradable biomembrane with the pore space of 80 to 200 micrometers and the thickness of 0.05 to 1.5 millimeters. Clean sewn tendons or ligaments are covered by adopting a packaging and winding method in operation. Patients can make proper early movement after operation. A peripheral tissue forms a neomembrane on a collagen membrane to prevent the adhesion of the peripheral tissue and a tendon, so that the purpose of preventing the adhesion of tendons and ligaments is achieved.
Description
Affiliated technical field:
The present invention relates to medical biotechnology material technology field, be specifically related to a kind of biomembranous preparation and application that tendon, ligament injury and postoperative is prevented adhesion.
Background technology:
Continuous development along with medical science development of technology and medical material, obtained significant development at medical field based on the product of collagen protein in recent years, as Chinese patent, the patent No. is 021212779, patent name is that medicinal collagen material and manufacturing process thereof disclose a kind of medicinal collagen material, with the cattle tendon tissue is base material, through remove impurity, clean, acid, alkali, enzyme is handled, the chondroitin sulfate agent is crosslinked, operation such as lyophilization and ethylene oxide sterilizing is made has hole, spongy medicinal collagen material has Stability Analysis of Structures, good biocompatibility, the characteristics that degradable absorbs.At present, be used for cerebral dura mater, spinal dural reparation and anti clinically.Use the expansion of this material and technology and extend to the important topic that the other treatment field is a biomedical sector.
At present; solve and recover the tendon of Orthopeadic Surgery hand because of tendon and ligament injury postoperative; the ligament adhesion is the difficult problem that global urgent need solves; influence the normal activity in joint because of the formation of cicatrix causes the adhesion of tendon and ligament behind the bone surgery, the no stndon sheath tendon injury more than 90% need be carried out for the first time the operation of loosening of selecting a time of the secondary of postoperative.At present, the dexamethasone hormone of clinical use and the therapeutic effect of biological fibrin glue and other materials are not obvious, can not effectively prevent the formation of adhesion.
Summary of the invention:
The purpose of this invention is to provide a kind of have hole, spongy medicinal collagen material, have the preparation method and the application that are used for tendon, ligament anti blocking biomembrane of Stability Analysis of Structures, good biocompatibility, degradable absorption.
In order to achieve the above object, the present invention adopts following technical scheme: by the cattle tendon is base material, through peracid, alkali, enzyme handle and chondroitin sulfate crosslinked, make after lyophilization, the sterilization purity of protein more than 95%, hole is the spongy type i collagen protein biology film of 80～200 μ m, thickness 0.05～1.5mm.When tendon that carries out hand or other positions and operation on ligament after it is sewed up, with the direct tubular ring of anti blocking biomembrane around covering on the impaired tendon or ligament, the extraneous tissue of blocking-up directly contacts with it, tendon outside organization produces pseudomembrane because of the special construction of collagem membrane makes it, stops growing into of outside cell and forms adhesion.
Its purity of protein height of type i collagen protein material biomembrane provided by the present invention, molecular structure stabilized, there are specific pore structure and tissue that good biocompatibility and controllable degraded and absorbed are arranged, be used for preventing tendon and ligament adhesion behind the bone surgery, provide curative effect reliable, easy to use, be with a wide range of applications.
The specific embodiment:
Provided by the present invention is that the biomembrane that prevents tendon and ligament adhesion of material proves that in zoopery preventing adhesiving effect is remarkable with type i collagen albumen.Specific implementation method is: by the cattle tendon is base material, through peracid, alkali, enzyme handle and chondroitin sulfate crosslinked, make after lyophilization, the sterilization purity of protein more than 95%, hole is that the spongy type i collagen albumen anti blocking biomembrane of 146 μ m, thickness 0.2mm is standby; Extremity tendon that animal is damaged and ligament undergo surgery sew up after, with the direct tubular ring of the anti blocking biomembrane of 20 * 30mm around covering on the impaired tendon or ligament; Crust is sewed up and planter cast then.The extremity of 2 week of operation back passive activity damage are removed Gypsum Fibrosum all around, continue the extremity of passive activity damage.12 weeks were observed sacrifice of animal, anti blocking biomembrane has played the extraneous tissue of blocking-up and its effect that directly contacts, tendon and ligament outside organization produce pseudomembrane because of the special construction of collagem membrane makes it, stop growing into of outside cell and form adhesion, and preventing adhesiving effect is remarkable.
Claims (3)
1. the preparation method of a tendon, ligament anti blocking biomembrane is a base material by the cattle tendon, and through peracid, alkali treatment, Protease Treatment and chondroitin sulfate are crosslinked, and making hole behind the dry sterilization is 146 μ m, and thickness is the spongy collagen protein biomembrane of 0.2mm.
2. a kind of tendon, the ligament anti blocking biomembrane that make of the described method of claim 1, it is characterized in that: the biomembranous hole of spongy collagen protein is 146 μ m, and thickness is 0.2mm.
3. the application of the anti blocking biomembrane that makes of the described method of claim 1 in preparation tendon, ligament anti blocking biomembrane.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004101015975A CN100366303C (en) | 2004-12-24 | 2004-12-24 | Preparation and application of anti blocking biomembrane for tendon and ligament |
| PCT/CN2005/002012 WO2006066483A1 (en) | 2004-12-24 | 2005-11-25 | Adhesion preventing biologic film for tendon ligament and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004101015975A CN100366303C (en) | 2004-12-24 | 2004-12-24 | Preparation and application of anti blocking biomembrane for tendon and ligament |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1795933A CN1795933A (en) | 2006-07-05 |
| CN100366303C true CN100366303C (en) | 2008-02-06 |
Family
ID=36601371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004101015975A Active CN100366303C (en) | 2004-12-24 | 2004-12-24 | Preparation and application of anti blocking biomembrane for tendon and ligament |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN100366303C (en) |
| WO (1) | WO2006066483A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102078641A (en) * | 2011-01-07 | 2011-06-01 | 北京天新福医疗器材有限公司 | Method for preparing anti-adhesive membrane for tendon ligaments |
| CN103341211B (en) * | 2013-06-14 | 2014-10-29 | 张仲文 | Preparation method for I/II collagen double-layer composite collagen membrane |
| CN103393482B (en) * | 2013-08-14 | 2016-04-06 | 北京瑞健高科生物科技有限公司 | A kind of mammary prostheses supporting device based on tissue matrix material and preparation method thereof |
| CN105524965A (en) * | 2016-02-26 | 2016-04-27 | 福建华灿制药有限公司 | Extraction method of bovine tendon collagen |
| CN107335101B (en) * | 2017-08-07 | 2022-10-04 | 北京湃生生物科技有限公司 | Composite collagen tissue regeneration membrane and preparation method thereof |
| CN110893250B (en) * | 2019-10-11 | 2022-03-15 | 许和平 | Scar/adhesion barrier film and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10216214A (en) * | 1996-11-13 | 1998-08-18 | Katsunari Nishihara | Medical material |
| WO2000032225A1 (en) * | 1998-12-03 | 2000-06-08 | University College London | Tissue repair |
| US20020028181A1 (en) * | 2000-04-28 | 2002-03-07 | Miller Mark E. | Polyacid/polyalkylene oxide foams and gels and methods for their delivery |
| CN1456362A (en) * | 2002-05-09 | 2003-11-19 | 中国人民解放军军事医学科学院野战输血研究所 | Artificial skin, preparing method and application thereof |
| CN1463984A (en) * | 2002-06-10 | 2003-12-31 | 于海鹰 | Medicinal collagen material and its making process |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1278868B1 (en) * | 1995-10-27 | 1997-11-28 | Sanitaria Scaligera Spa | METHOD FOR OBTAINING A REABSORBABLE MATERIAL SUITABLE TO BE USED AS A COATING ELEMENT FOR THE PREVENTION OF |
| CN1539515A (en) * | 2003-11-03 | 2004-10-27 | 刘永庆 | Biomaterial for preventing accretion after surgery and preparation method |
-
2004
- 2004-12-24 CN CNB2004101015975A patent/CN100366303C/en active Active
-
2005
- 2005-11-25 WO PCT/CN2005/002012 patent/WO2006066483A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10216214A (en) * | 1996-11-13 | 1998-08-18 | Katsunari Nishihara | Medical material |
| WO2000032225A1 (en) * | 1998-12-03 | 2000-06-08 | University College London | Tissue repair |
| US20020028181A1 (en) * | 2000-04-28 | 2002-03-07 | Miller Mark E. | Polyacid/polyalkylene oxide foams and gels and methods for their delivery |
| CN1456362A (en) * | 2002-05-09 | 2003-11-19 | 中国人民解放军军事医学科学院野战输血研究所 | Artificial skin, preparing method and application thereof |
| CN1463984A (en) * | 2002-06-10 | 2003-12-31 | 于海鹰 | Medicinal collagen material and its making process |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2006066483A1 (en) | 2006-06-29 |
| CN1795933A (en) | 2006-07-05 |
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