CN100364612C - Degraded agonist antibody - Google Patents

Abstract

The invention relates to a modified antibody which contains two or more H chain V regions and two or more L chain V regions of monoclonal antibody and can transduce a signal into cells by crosslinking TPO receptor to thereby exert TPO agonist action. The modified antibody can be used as a TPO signal transduction agonist and, therefore, useful as a preventive and/or remedy for various diseases such as platelet-reduction-related blood diseases, thrombopenia following chemotherapy for cancer or leukemia, etc.

Description

Degraded agonist antibody

The application is that the application number of October 22 calendar year 2001 application is dividing an application of 01817431.0 Chinese invention patent application " degraded agonist antibody ".

Technical field

The antibody that the present invention relates to change, it comprises two or more H chain V districts and two or more L chains V district of monoclonal antibody, and this monoclonal antibody shows agonist activity by molecule in crosslinked cell surface molecule or the cell.The antibody of this change has the agonist activity that signal is changed over to cell by crosslinked cell surface molecule, as multi-purpose medicine.

Background technology

JP-A 9-295999 discloses with the preparation of spleen stromal cell lines as the antigenic monoclonal antibody specific of sensitization, its purpose is to develop the specific antibody that can discern above-mentioned spleen stromal cell, and discloses the preparation of mice integrin associated protein matter (mice IAP) as the novel monoclonal antibody of antigen recognition.JP-A.9-295999 also discloses these monoclonal antibodies can induce the medullary cell apoptosis.

It is that people's integrin associated protein matter (is quoted the IAP into the people herein that WO99/12973 discloses its antigen; Its aminoacid sequence and nucleotide sequence are described in J.Cell Biol., 123,485-496,1993; Also referring to Journal of cell Science, 108,3419-3425,1995) monoclonal antibody, it can induce people's nucleated blood cell (medullary cell and the lymphocyte) apoptosis with described people IAP.These monoclonal antibodies are called as antibody MABL-1 and antibody MABL-2, and the hybridoma that produces these antibody also is called MABL-1 (FERM BP-6100) and MABL-2 (FERM BP-6101).

Japanese patent application 11-63557 has described that to have with people IAP be the preparation of strand Fv in the strand Fv district of antigenic monoclonal antibody.This strand Fv can induce the nucleated blood cell apoptosis with people IAP.

IAP is as antigenic monoclonal antibody in identification, can induce the nucleated blood cell apoptosis with people IAP, but also cause ExternalHemagglutination reaction.Show that giving to discern in a large number IAP is that antigenic monoclonal antibody may have side effects, for example hemagglutination reaction.

The inventor utilizes the monoclonal antibody of people IAP to further investigate as the medicine of hematologic disease, has obtained to have the strand Fv in the strand Fv district that can induce the nucleated blood cell apoptosis with people IAP.

On the other hand, the antibody that the antibody of change, particularly molecular size reduce has for example been developed raising by the reduction molecular size and has been entered the strand Fv of tissue and tumor permeability, and passed through recombinant methods.Recently, the dimer of strand Fv, particularly the bispecific dimer has been used for crosslinked cell.This dimeric exemplary is the heterodimer of strand Fv, and its identification cancer cell antigen and host cell are as the antigen of NK cell and neutrophil cell people such as (, Int.J.Cancer, 77,9763-9772,1998) Kipriyanov.They utilize strand Fv constructing technology to be prepared as the antibody of change, by crosslinked between inducing cell and more effective treatment cancer.Thought that the crosslinked antibody that is changed by antibody and fragment (for example Fab fragment) thereof, bispecific of iuntercellular and even the strand Fv dimer of monospecific induce.

For passing through crosslinked cell surface molecule and the antibody of transduction signal, known have the antibody (JP-A 2000-95800) of cell differentiation and the EPO receptor that relates to of propagation, the antibody of a MuSK receptor (people such as Xie, Nature Biotech.15,768-771,1997) and other.But the report that does not still have the antibody of the change that reduces molecular size.

Notice from the strand Fv monomer of MABL-1 antibody and MABL-2 antibody cell death inducing not, and strand Fv dimer is induced the apoptosis with IAP, the inventor finds IAP receptor on its crosslinked (Dimerized) cell surface, thereby transduction signal enters cell, causes apoptosis-induced.This prompting, the crosslinked cell surface molecule of monospecific strand Fv dimer (for example receptor), as the aglucon transduction signal, thereby as agonist.

The crosslinked discovery of research iuntercellular, above-mentioned strand Fv dimer does not cause hemagglutination reaction, said monoclonal antibody then can.Strand bivalent antibody (single chain polypeptide that comprises two H chain V districts and two L chain V districts) is also observed identical result.This prompting, it is crosslinked that monoclonal antibody may form iuntercellular, and the antibody that changes, as strand Fv dimer and strand bivalent antibody, crosslinked cell surface molecule but not form iuntercellular crosslinked.

Based on these observations, inventor's recent findings, the antibody that changes, for example strand Fv dimer and strand bivalent antibody, except known iuntercellular is crosslinked, crosslinked cell surface molecule or isocellular iuntercellular molecule, be suitable as this molecule aglucon (particularly as the simulation natural aglucon effect aglucon).

Can change by further discovery antibody molecule (fully IgG) becomes the strand of crosslinked cell surface molecule Fv dimer, strand bivalent antibody etc., thereby reduce the side effect of the crosslinked generation of iuntercellular, and a new drug of inducing cell purpose effect is provided, the inventor has finished the present invention.Compare natural aglucon, for example TPO, EPO or G-CSF perhaps have the complete antibody (IgG) in the V district identical with the antibody that changes, and the antibody of change of the present invention has very high activity.Owing to compare antibody molecule, its molecular size reduces, and lacks constant region, and its permeability that enters tissue improves.

Summary of the invention

One object of the present invention is to provide the antibody of the agonist change of hanging down molecular size, it comprises two or more H chain V districts and two or more L chains V district of monoclonal antibody, and has the agonist effect by molecule in crosslinked cell surface molecule or the cell.

Therefore, the antibody that the present invention relates to change, it comprises two or more H chain V districts and two or more L chains V district, preferred every kind of 2-6, preferred especially every kind of 2-4, most preferably two every kind, and show agonist activity by molecule in crosslinked cell surface molecule or the cell.

" antibody of change " is meant any material that comprises two or more H chain V districts and two or more L chains V district in this description, and wherein said V district directly or by joint connects with covalent bond or non-covalent bond.For example, connect polypeptide and the chemical compound that each V district of antibody prepares by peptide linker or chemical cross-linking agent etc.Two or more H chain V districts and two or more L chains V district that the present invention uses can come from same antibody or different antibodies.

The preferred embodiment of the antibody that the present invention changes is the polymer that comprises the strand Fv in H chain V district and L chain V district, and for example dimer, trimer or the tetramer perhaps comprise the single chain polypeptide in two or more H chain V districts and two or more L chains V district.If the antibody of change of the present invention is the polymer that comprises the strand Fv in H chain V district and L chain V district, for example dimer, trimer or the tetramer etc. preferably are in H chain V district on the same chain and L chain V district not in conjunction with forming antigen binding site.

Preferred example is the dimer that comprises the strand Fv in H chain V district and L chain V district, perhaps comprises the single chain polypeptide in two H chain V districts and two L chain V districts.H chain V district and L chain V district preferably link by joint in the antibody that changes.

In this description " agonist effect " be meant by molecule in crosslinked cell surface molecule or the cell and change the biological action that takes place in the cell of signal over to, for example apoptosis-induced, inducing cell propagation, inducing cell differentiation, inducing cell division or Cycle Regulation effect.

By measuring the known method of agonist effect, determine the ED50 of agonist effect of the present invention.For example special cell death or the cell proliferation of detection of agonist detects the expression (for example specific antigen) of cell differentiation specific protein, perhaps measures the special kinase activity of cell cycle.ED50 is for reaching 50% required dosage of maximum activity (it is made as 100% in dose-response curve).

The antibody that the present invention preferably changes, its agonist effect (ED50) is equal to or better than the antibody with antigen binding domain identical with the antibody that changes, promptly have with the H chain V district of the antigen binding domain of the antibody that form to change and L chain V district to the right complete antibody in identical H chain V district and L chain V district, as IgG (following " parental generation antibody ").More preferably its agonist effect (ED50) is higher than parental generation antibody more than two times, further preferred more than 5 times, the antibody of the change more than 10 times most preferably.The present invention comprises the antibody of the change with agonist effect, and it comprises H chain V district and the L chain V district that forms the antigen binding domain identical with parental generation antibody (can be in target cell surface molecular or cell molecule, but this molecule is not had the agonist effect).

The chemical compound that comprises two or more H chain V districts and two or more L chains V district of the present invention can be any chemical compound that the agonist effect (ED50) that comprises two or more H chain V districts of antibody and two or more L chains V district, show is equal to or better than the natural aglucon of molecule in cell surface binding molecule or the cell.Be preferably agonist effect (ED50) and surpass natural aglucon more than 2 times, more preferably above more than 5 times, most preferably above the chemical compound more than 10 times.

" chemical compound " described herein not only comprises the antibody of change of the present invention, also comprise comprise two or more, preferred 2-6, more preferably 2-4, any chemical compound of 2 antigen binding domains most preferably, for example complete antibody or F (ab ') ₂

Antibody or chemical compound that the present invention comprises the change in two or more H chain V districts of antibody and two or more L chains V district preferably do not have basic intercellular adhesion effect.When the H chain V district of the antibody that changes as the present invention and L chain V district derived from same antibody, the intercellular adhesion effect (ED50) that it preferably has is no more than compared 1/10 of original antibody.

Utilize the known method of measuring the agonist effect to determine the ED50 of intercellular adhesion effect of the present invention, for example by measuring the agglutination of the cell of expressing described cell surface molecule, for example hemagglutination test.

The DNA of the antibody that changes the present invention relates to encode.

The present invention relates to the zooblast or the microorganism of mutagenic antibody.

The antibody that the present invention relates to change is as the purposes of agonist.

Thereby the present invention relates to the method that by molecule in the antibody linked cell surface molecule that use to change or cell transduction signal enters cell inducing cell agonist effect (for example apoptosis-induced, inducing cell propagation, inducing cell differentiation, inducing cell division or Cycle Regulation effect).

The present invention relates to comprise the medicine of the antibody of change.

The antibody that the present invention relates to change is as the purposes of medicine.

The present invention relates to the screening or the assay method of the antibody of the change that comprises two or more H chain V districts and two or more L chain V districts of antibody and show the agonist effect by molecule in crosslinked cell surface molecule or the cell, it comprises 1) preparation comprises two or more H chain V districts of antibody and two or more L chain V districts and specificity antibody in conjunction with the change of described molecule, 2) cell and 3 of the described molecule of antibody contact expression that changes) mensuration causes the agonist effect that takes place in the cell by crosslinked described molecule.Assay method is used for preparing the quality control of the present invention as the antibody of the change of medicine and other purpose.

Above-mentioned strand Fv dimer comprises non-covalent bond dimer, the covalent bond dimer by crosslinking group and the dimer (antibody that antibody, antibody fragment or two price-reform become) by cross-linking agent.The conventional crosslinking group that is used for crosslinked peptide can be as forming dimeric crosslinking group.For example crosslinked by the disulphide of cysteine residues, other crosslinking group is as C ₄-C ₁₀Alkylidene (for example tetramethylene, pentamethylene, hexa-methylene, heptamethylene and eight methylene etc.) or C ₄-C ₁₀Alkenylene (suitable/anti--3-butenylidene, suitable/anti--2-inferior pentenyl, suitable/anti--3-inferior pentenyl, suitable/inferior hexenyl of anti--3-etc.).

In addition, can have, for example can introduce the aminoacid sequence of Fv arbitrarily, for example antibody of FLAG sequence etc. or its fragment, or derive from the antibody of the change of antibody, for example strand Fv in conjunction with the cross-linking agent of strand Fv.

The invention still further relates to first aglucon and second aglucon, and give material, thereby induce the method for pair cell agonist effect in conjunction with first and second aglucons and crosslinked first and second aglucons by giving molecule in cell surface binding molecule or the cell.First aglucon and second aglucon can be comprise binding site to described molecule, and can be via crosslinked and induce any material of agonist effect.Preferred embodiment is the antibody that unit price changes, for example identical or different strand Fv monomer, antibody fragment etc.The material of crosslinked above-mentioned aglucon can be any material of inducing the effect of pair cell agonist by crosslinked first aglucon and second aglucon.Preferred embodiment is antibody, antibody fragment, (Fab) ₂Or the antibody of two price-reforms changes.The example of bivalent antibody is (Fab) ₂, comprise the strand Fv dimer in a H chain V district and a L chain V district and comprise two H chain V districts and the single chain polypeptide in two L chain V districts.This method can effectively be explored by crosslinked and transduction signal enters the receptor of cell, wishes to be used for drug delivery is gone into the DDS of target cell, also as suppressing side effect and making medicine effective drug-supplying system in the period of desired time and hope.

The antibody of change of the present invention can be to comprise the L chain V district of antibody (for example antibody MABL-1, antibody MABL-2, antibody 12B5, antibody 12E10 etc.) and any material in H chain V district, and molecule in specific recognition cell surface molecule or the cell (for example sugar chain of protein (receptor or participate in the protein of signal transduction) or above-mentioned protein or cell membrane protein) thereby and crosslinked described cell surface molecule transduction signal enter any material of cell.The antibody that comprises the change that a part of aminoacid sequence in V district has wherein changed.

According to the characteristic for the treatment of molecule in bonded cell surface molecule or the cell, for example molecular structure or mechanism of action, the antibody of this change can be (as the bispecific) of monospecific or polyspecific.But if the acceptor molecule (for example erythropoietin receptor, thrombopoietin receptor, G-CSF receptor, scf receptor, EGF receptor, IAP (CD47) etc.) that antibodies homodimerization that changes and transduction signal enter cell, the antibody that preferred monospecific changes.If but its combination heterodimerization and transduction signal enter the acceptor molecule (for example IL-6 receptor, LIF receptor, IL-11 receptor) of cell, the antibody that preferred bispecific changes.If but its combination heterotrimerization and transduction signal enter the acceptor molecule (for example IL-2 receptor, CNTF receptor, OSM receptor) of cell, the antibody that preferred tri-specific changes.Preparation bispecific strand Fv dimeric method is as described in the WO9413804 etc.

The invention still further relates to H chain V district and/or L chain V district and be the antibody of the change in the people's light chain of antibody V district that derives from the H chain V district of people's antibody and/or derive from.The H chain V district and/or the L chain V district that derive from people's antibody can obtain by screening human monoclonal antibodies library, as described in WO99/10494.Also comprise the H chain V district and the L chain V district that derive from human monoclonal antibodies.

The invention further relates to H chain V district and/or L chain V district is the antibody of the change in humanized H chain V district and/or humanized L chain V district.Particularly, the antibody of this humanization change is made up of humanized L chain V district (complementary determining region (following " CDR ") that it comprises the framework region (FR) that derives from human monoclonal antibodies L chain V district and derives from non-human mammal (for example mice, rat, cattle, sheep, ape) monoclonal antibody L chain V district) and/or humanized H chain V district (CDR that it comprises the FR that derives from human monoclonal antibodies H chain V district and derives from non-human mammal (for example mice, rat, cattle, sheep, ape) monoclonal antibody H chain V district).In this case, can partly change the aminoacid sequence of CDR and FR, for example lack, replace or add.

The H chain V district and/or the L chain V district of the antibody that the present invention changes can be H chain V district and/or the L chain V districts that derives from the monoclonal antibody of animal except that the people (for example mice, rat, cattle, sheep, ape, chicken etc.).In this case, can partly change the aminoacid sequence of CDR and FR, for example lack, replace or add.

The DNA of antibody of above-mentioned various changes and the technique for gene engineering that is used to prepare the recombinant vector that comprises this DNA the present invention also relates to encode.

The invention still further relates to and use the recombinant vector transformed host cells.The example of host cell is a zooblast, for example people's cell, mouse cell etc., and microorganism, for example escherichia coli ( E. Coli), Bacillus subtilis (Bacillus subtilis), yeast etc.

The present invention relates to the method for mutagenic antibody, comprise and cultivate above-mentioned host, and from its culture, extract the antibody of this change.

The present invention relates in addition and produces the dimeric method of strand Fv, be included in and cultivate the host animal cell that can produce this strand Fv in the serum-free medium, with secretion strand Fv in culture medium, and the strand Fv dimer that forms in the isolation medium.

The invention still further relates to the purposes of the antibody of this change as agonist.That is to say, relate to the signal transduction agonist, it comprises the antibody as the change of active component of above-mentioned acquisition.Owing to be used for molecule and inducement signal transduction in the antibody linked cell surface molecule of change of the present invention or the cell, this molecule can be by in conjunction with aglucon oligomerization (for example Dimerized) thus transduction signal enters any molecule of cell.

This type of cell surface molecule comprises hormone receptor and cytokine receptor.Hormone receptor comprises, for example estrogen receptor.Cytokine receptor etc. comprise Hemopoietic factor receptor, lymphokine receptor, growth factor receptors, differentiation controlling elements receptor etc.The example of cytokine receptor has erythropoietin (EPO) receptor, thrombopoietin (TPO) receptor, granulocyte colony-stimulating factor (G-CSF) receptor, M-CSF (M-CSF) receptor, granulocyte macrophage colony stimulating factor (GM-CSF) receptor, tumor necrosis factor (TNF) receptor, interleukin-1 (IL-1) receptor, interleukin II (IL-2) receptor, interleukin 3 (IL-3) receptor, interleukin 4 (IL-4) receptor, interleukin 5 (IL-5) receptor, interleukin-6 (IL-6) receptor, interleukin 7 (IL-7) receptor, interleukin 9 (IL-9) receptor, interleukin 10 (IL-10) receptor, interleukin 11 (IL-11) receptor, interleukin 12 (IL-12) receptor, Interleukin-13 (IL-13) receptor, Interleukin-15 (IL-15) receptor, interferon-' alpha ' (IFN-α) receptor, interferon-beta (IFN-β) receptor, interferon-(IFN-γ) receptor, growth hormone (GH) receptor, Insulin receptor INSR, blood stem cell multiplicaiton factor (SCF) receptor, vascular endothelial cell growth factor (VEGF) receptor, epithelial cell growth factor (EGF) receptor, nerve growth factor (NGF) receptor, fibroblast growth factor (FGF) receptor, platelet-derived growth factor (PDGF) receptor, transforming growth factor-beta (TGF-β) receptor, leukocyte migration inhibition factor (LIF) receptor, ciliary neurotrophic factor (CNTF) receptor, oncostatin M (OSM) receptor, Notch family receptors etc.

Cell inner surface molecule comprises TAK1, TAB1 etc.TAK1 and TAB1 act on the signal transduction pathway of TGF-β, by forming heterodimer activation map kinase, transduction a series of signal.The TGF-beta receptor of the inhibition cancer growth of many cancerous cell has sudden change, the TGF-signal beta of therefore can not transduceing.Can crosslinked TAK1 and the antibody of the change of TAB1 and transduction signal, can induce the TGF-signal beta by agonist effect in conjunction with TAK1/TAB1.The antibody of this change of the present invention can suppress the growth of TGF-β resistance cancerous cell, and the new method of treatment cancer is provided.Other example of molecule is transcription factor E2F homodimer and the E2F/DP1 heterodimer with cel l proliferation in the cell.The antibody of change of the present invention also can be induced the agonist effect to these molecules, therefore can be used in the relevant disease of the various cell proliferation of treatment.The antibody of change of the present invention also can be induced the agonist effect by the crosslinked cell intrinsic factor that relates in the relevant signal transduction of apoptosis induction, therefore can inducing cancer cell or the apoptotic cell death of autoimmune disease relevant cell.

Interaction for molecule in the antibody of realizing change of the present invention and the cell, peptide (Pegelin for example with the penetrating ability of cell membrane, Penetratin) antibody that can be used for transporting this change enters cell (people such as Martine Mazel, Doxorubicin-peptide conjugatesovercome multidrug resistance.Anti-Cancer Drugs 2001,12, DccrossiD. wait the people, The third helix of the antennapedia homeodomaintranslocates through biological membranes, J.Biol.Chem.1994,269,10444-10450).

Therefore, the pharmaceutical preparation that comprises antibody that agonist changes and be active component is as the prevention of various diseases and medicine etc., for example cancer, inflammation, hormone disturbance, hematologic disease and autoimmune disease.

The oligomer that can be formed by receptor protein can be homology oligomer or oligomeric body, and any oligomer, for example dimer, trimer and the tetramer.For example, known erythropoietin receptor, thrombopoietin receptor, G-CSF receptor, scf receptor, EGF receptor etc. form homodimer, IL-6 receptor, LIF receptor and IL-11 receptor form heterodimer, and IL-2 receptor, CNTF receptor, OSM receptor form heterotrimer.

The antibody of change of the present invention comprises 2 or a plurality of H chains V district and 2 or a plurality of L chain V district that comes from monoclonal antibody.The structure of the antibody of this change can be the dimer that comprises the strand Fv in 1 H chain V district and 1 L chain V district, or comprises the polypeptide in 2 H chain V districts and 2 L chain V districts.In the antibody of change of the present invention, the V district of H chain and L chain preferably links to each other by the peptide linker that one or more aminoacid are formed.The antibody of the change that produces comprises the variable region of antibody, with the specificity conjugated antigen identical with original monoclonal antibody.

H chain V district

Among the present invention, derive from the H chain V district's recognizing cells surface molecular or the interior molecule of cell of antibody, the sugar chain on protein (receptor or signal transduction related protein) or protein or the cell membrane for example, and oligomer, for example by crosslinked described molecule and Dimerized, thereby transduction signal enters cell.H chain V of the present invention district comprises the H chain V district that derives from mammal (for example people, mice, rat, cattle, sheep, ape etc.), and the H chain V district with the aminoacid sequence in the H chain V district that part changes.Be more preferably humanized H chain V district, it comprises the FR in human monoclonal antibodies H chain V district, and the CDR in mouse monoclonal antibody H chain V district.The H chain V district that also preferably has the aminoacid sequence that comes from the people, it can utilize the recombinant technique preparation.H chain V of the present invention district may be the fragment that keeps the above-mentioned H chain V district of antigen binding capacity.

L chain V district

Among the present invention, molecule in L chain V district's recognizing cells surface molecular or the cell, for example sugar chain on protein (receptor or signal transduction related protein) or protein or the cell membrane, and oligomer, for example by crosslinked described molecule and Dimerized, thereby transduction signal enters cell.L chain V of the present invention district comprises the L chain V district that derives from mammal (for example people, mice, rat, cattle, sheep, ape etc.), and the L chain V district with the aminoacid sequence in the L chain V district that part changes.Be more preferably humanized L chain V district, it comprises the FR in human monoclonal antibodies L chain V district, and the CDR in mouse monoclonal antibody L chain V district.The L chain V district that also preferably has the aminoacid sequence that comes from people's antibody, it can utilize the recombinant technique preparation.L chain V of the present invention district may be the fragment that keeps the L chain V district of antigen binding capacity.

Complementary determining region (CDR)

Each V district of L chain and H chain forms antigen binding site.(Kabat is formed by 4 the quite conservative common framework regions that are connected to 3 hypervariable regions or complementary determining region (CDR) in the variable region of L and H chain, E.A. wait the people, " Sequences of Protein ofImmunological Interest ", US Dept.Health and Human Services, 1983).

Major part in 4 framework regions (FR) forms β-lamellar structure, thereby 3 CDR form ring.Under some situation, CDR may form the part of lamellar structure.3 CDR are near each other by FR on the locus, form antigen in conjunction with node together with 3 CDR.

According to Kabat, the empirical rule among the people such as E.A. " Sequences of Protein of ImmunologicalInterest " compares the antibody V region amino acid sequence of acquisition and the known amino acid sequence in known antibodies V district, identifies these CDR.

Strand Fv

Strand Fv is a polypeptide monomer, comprises interconnected H chain V district and the L chain V district that comes from monoclonal antibody.The strand Fv that produces comprises the variable region of parent's monoclonal antibody, and keeps its complementary determining region, so this strand Fv is by the specificity conjugated antigen (JP-Appl.11-63557) identical with parent's monoclonal antibody.A part of variable region and/or the CDR of strand Fv of the present invention, or the one partial amino-acid series may partly change, and for example lacks, replaces or add.Before addressed H chain V district and the L chain V district that constitutes strand Fv of the present invention, they can directly connect or be continuous by joint, the preferred peptide joint.The structure of strand Fv can be [H chain V district]-[L chain V district], perhaps [L chain V district]-[H chain V district].Among the present invention, might make strand Fv form dimer, trimer or the tetramer, therefrom form the antibody of change of the present invention.

The antibody that strand changes

The present invention comprises the antibody of two or more H chains V district and two or more L chain V district (preferred every kind of 2-4 's, preferred especially 2 every kind) strand change, comprises above-mentioned two or more H chains V district and L chain V district.Should arrange each district of peptide, make the single-chain antibody of change form specific space structure, accurately simulate the space structure that forms by strand Fv dimer.For example, sequence arrangement V district in the following manner: [H chain V district]-[L chain V district]-[H chain V district]-[L chain V district]; Perhaps [L chain V district]-[H chain V district]-[L chain V district]-[H chain V district], wherein these districts link to each other by peptide linker respectively.

Joint

Among the present invention, connecting joint between H chain V district and the L chain V district and may be can be by any peptide linker of gene engineering method introducing, or any joint of chemosynthesis.For example, the present invention can use disclosed joint in the document, as Protein Egineering, and 9 (3), 299-305,1996.These joints can be identical or different in a part.Peptide linker if desired, below joint example for quoting:

Ser

Gly-Ser

Gly-Gly-Ser

Ser-Gly-Gly

Gly-Gly-Gly-Ser

Ser-Gly-Gly-Gly

Gly-Gly-Gly-Gly-Ser

Ser-Gly-Gly-Gly-Gly

Gly-Gly-Gly-Gly-Gly-Ser

Ser-Gly-Gly-Gly-Gly-Gly

Gly-Gly-Gly-Gly-Gly-Gly-Ser

Ser-Gly-Gly-Gly-Gly-Gly-Gly

(Gly-Gly-Gly-Gly-Ser) _nAnd

(Ser-Gly-Gly-Gly-Gly) _n

Wherein n is not less than 1 integer.Preferred peptide linker length is according to changing as antigenic receptor, for strand Fv, in preferred usually 1-20 the aminoacid scope.Antibody for the strand change that comprises two or more H chains V district and two or more L chain V district, the peptide linker length that is connected to form the same antigen binding site that comprises [H chain V district]-[L chain V district] (or [L chain V district]-[H chain V district]) with it is 1-30 aminoacid, preferred 1-20 aminoacid, more preferably 3-18 aminoacid.The peptide linker length that is connected with it and does not form the same antigen binding site that comprises [H chain V district]-[L chain V district] (or [L chain V district]-[H chain V district]) is 1-40 aminoacid, preferred 3-30 aminoacid, more preferably 5-20 aminoacid.In the explanation of the DNA construct of the antibody that code book invention changes, the method for introducing these joints will be described.

According to the present invention, the joint of chemosynthesis, promptly chemical cross-linking agent can be any joint that routine is used for connection peptides.The example of joint comprises N-hydroxy-succinamide (NHS), disuccinimidyl suberate (DSS), two (sulfosuccinimide the ester) (BS of suberic acid ³), dithio two (propanoic acid succinum imines ester) (DSP), dithio two (propanoic acid sulfosuccinimide ester) (DTSSP), ethylene glycol bis (succinic acid succinimide ester) (EGS), ethylene glycol bis (succinic acid sulfosuccinimide ester) (sulfo-EGS), tartaric acid two succinimide esters (DST), tartaric acid disulfo succinimide ester (sulfo-DST), two [2-(succinimido oxygen ketonic oxygen base) ethyl] sulfone (BSOCOES), two [2-(sulfosuccinimide base oxygen ketonic oxygen base) ethyl] sulfone (sulfo-BSOCOES) etc.These all are commercial.Preferably identical chemosynthesis joint with peptide linker length.

For forming the dimer of strand Fv, preferred joint is suitable in solution, and the strand Fv that host cell is produced is Dimerized to surpass 20%, preferably surpasses 50%, more preferably surpasses 80%, most preferably surpasses 90%.Especially, preferred joint is made up of 2-12 aminoacid, preferred 3-10 aminoacid, or other corresponding joint.

The preparation of the antibody that changes

By connect the H chain V district and the L chain V district of the known or novel monoclonal antibody that derives from the specificity cell surface binding molecule with above-mentioned joint, can prepare the antibody of change.The example of the strand Fv that relates to has MABL1-scFv and MABL2-scFv, and it comprises H chain V district and the L chain V district that derives from antibody MABL-1 and antibody MABL-2 respectively.The example of the single chain polypeptide that comprises two H chain V districts and two L chain V districts that relates to has and comprises the H chain V district that derives from above-mentioned antibody and the MABL1-sc (Fv) in L chain V district ₂And MABL2-sc (Fv) ₂

For preparing this polypeptide, be the secretion peptide if wish this polypeptide, then can connect signal peptide at polypeptide N end.Be effective purified polypeptide, can connect the well-known aminoacid sequence that is used for peptide purification, for example the FLAG sequence.In the case, utilize anti-FLAG antibody can form dimer.

For preparing the antibody of change of the present invention, must obtain DNA, the DNA of the DNA of the strand Fv that promptly encodes or the single chain polypeptide of coding and rebuilding.These DNA, particularly MABL1-scFv, MABL2-scFv, MABL1-sc (Fv) ₂And/or MABL2-sc (Fv) ₂DNA, can obtain the DNA that own coding derives from H chain V district and the L chain V district of described Fv.They also can obtain by PCR method, and it utilizes these DNA to be template, and the primer of corresponding its two end is right, and amplification wherein comprises the DNA part of purpose aminoacid sequence.

When each V district of needs has the aminoacid sequence that part changes, can use PCR, by methods known in the art, obtain the V district of one of them or some amino acid changes, promptly lack, replace or increase.For preparing the antibody that specific antigen is had abundant active change, preferably utilize PCR method known in the art, change the partial amino-acid series in the V district.

For determining the primer of pcr amplification, must determine the H chain of purpose antibody and the type of L chain.Yet the existing report of antibody MABL-1 and antibody MABL-2, antibody MABL-1 is κ type L chain and γ 1 type H chain, antibody MABL-2 is κ type L chain and γ 2a type H chain (JP-APPl.11-63557).For the H chain of pcr amplification encoding antibody MABL-1 and/or antibody MABL-2 and the DNA of L chain, can use people such as Jones, S.T., Bio/Technology, 9,88-89,1991 described primers.

Be the L chain V district of pcr amplification antibody MABL-1 and antibody MABL-2, determine the oligonucleotide primers of 5 ' end and 3 ' end as stated above.In kind determine used 5 ' end and 3 ' the end oligonucleotide primers in H chain V district of amplification antibody MABL-1 and antibody MABL-2.

In embodiment of the present invention, 5 ' end primer of use comprises " GANTC " sequence, provides Restriction Enzyme Hinf I recognition site near its 5 ' end, and 3 ' end primer comprises nucleotide sequence " CCCGGG ", provides XmaI recognition site near its 5 ' end.As long as can be used for the target DNA fragment sub-clone in cloning vehicle, can use other Restriction Enzyme recognition site to replace these sites.

Use specially designed PCR primer, 5 ' end and 3 ' end at the V district of antibody MABL-1 and MABL-2 code cDNA provide suitable nucleotide sequence, thereby make cDNA insert expression vector easily and in expression vector, have appropriate functional (for example, the present invention design improves translation efficiency by inserting the Kozak sequence).The HEF expression vector (seeing WO92/19759) that the V district insertion that utilizes these primers to carry out antibody MABL-1 that pcr amplification obtains and MABL-2 is comprised purpose people C district.Can utilize any conventional method order-checking clone's DNA, for example utilize automated DNA sequenator (Applied Biosystems).

Can be by following method, with a joint, for example peptide linker is introduced the antibody of change of the present invention.Design has the part complementary series of above-mentioned H chain V district and L chain V district primer, and the primer of the N end of coding joint or C end.Then, utilize these primers to carry out PCR, preparation has the coding DNA of the peptide linker of purpose aminoacid sequence and length.The DNA that utilization obtains connects the coding DNA in H chain V district and L chain V district, and the preparation code book is invented the DNA of the antibody of the change with purpose peptide linker.In case prepared the DNA of the antibody of the change of encoding, by the various primers of designed joint, utilize primer and above-mentioned DNA to carry out PCR for template, can be easy to prepare the DNA of antibody that coding is with or without the change of purpose peptide linker.

Utilize routine techniques (for example, people such as Sato K., Cancer Res., 53,1-6 (1993)), each V district of the antibody that can change the present invention carries out humanization.In case prepared the DNA of each humanization Fv that encodes,, can be easy to prepare the fragment of fragment, Humanized monoclonal antibodies and the Humanized monoclonal antibodies of Humanized single chain Fv, Humanized single chain Fv according to conventional method.Preferably, if desired, can carry out part to its V region amino acid sequence and change.

In addition, utilize above-mentioned conventional method, come from other mammiferous DNA, the DNA in each V district of the people's antibody of for example encoding by preparing used the same manner of DNA that coding comes from H chain V district and the L chain V district of mice, can preparing.The DNA that obtains can be used for preparing other mammal, particularly derives from H chain V district and L chain V district, the strand Fv that derives from the people and fragment and the human monoclonal antibody and the fragment thereof of people's antibody.

If the antibody of change of the present invention is the antibody that bispecific changes, then can prepare (for example described method of WO9413804) by known method.

As mentioned above, if prepared the purpose coding DNA in the V district of the V district of the antibody that coding changes and the antibody that humanization changes, just can comprise their expression vector and with this carrier host transformed according to the conventional method acquisition.In addition, can cultivate the host, with the strand Fv of preparation reconstruction, Humanized single chain Fv, Humanized monoclonal antibodies and the fragment thereof of reconstruction according to conventional method.It can separate from cell or culture medium, and purification is a uniform substance.For this purpose, can unite any conventional method that is used for Separation of Proteins and purification, for example, if desired, but be not limited to, chromatography, ultrafiltration, saltout and dialyse.

When in serum-free medium, cultivating zooblast, for example COS7 cell or Chinese hamster ovary celI, preferred Chinese hamster ovary celI, when preparing reconstruction strand Fv of the present invention, the dimer of the described strand Fv that forms in culture medium can be stablized and reclaims and the high yield purification.The dimer of purification can steady in a long-termly be preserved like this.The serum-free medium that the present invention uses can be any culture medium that routine is used to prepare recombinant protein, but is not limited thereto.

For preparing the antibody of change of the present invention, can utilize any expression system, for example, and eukaryotic cell such as zooblast, for example mammal cell line of Jian Liing, filamentous fungi and yeast, and prokaryotic cell such as bacterial cell, for example E.coliPreferably, in mammalian cell, express the antibody of change of the present invention, for example COS7 cell or Chinese hamster ovary celI.

In these cases, can use the conventional promoter that can be used for mammalian cell expression.The preferred immediate early promoter that uses Human Cytomegloviru (HCMV).The expression vector that comprises the HCMV promoter comprises (WO92/19759) such as HCMV-VH-HC γ 1, HCMV-VL-HCK that comes from pSV2neo.

In addition, spendable other promoter that can be used for the expression of mammalian cell group of the present invention comprises the viral promotors that derives from retrovirus, polyoma virus, adenovirus and simian virus 40 (SV40), and derive from mammiferous promoter, for example the human polypeptides chain elongation factor 1 α (HEF-1 α).(Nature 277 can to use the SV40 promoter easily according to people's such as Mulligan R.C. method, 108-114 (1979)), also can use HEF-1 α promoter (Nucleic Acids Research, 18,5322 (1990)) according to people's methods such as Mizushima S..

Can be used for origin of replication of the present invention (ori) and comprise the starting point that derives from SV40, polyoma virus, adenovirus, bovine papilloma virus (BPV) etc.Expression vector can comprise phosphotransferase APH (3 ') II or I (neo) gene, thymidine kinase (TK) gene, E.coliXanthine-guanine phosphoribosyl transferase (Ecogpt) gene or dihydrofolate reductase (DHFR) gene are as selection marker.

Utilize conventional method such as radioimmunoassay, RIA (RIA), Enzyme Linked Immunoadsorbent Assay (ELISA) or surperficial cytogene resonance technique (surface plasmon resonance), can estimate the antigen-binding activity of antibody of the change of above-mentioned preparation.Also can utilize the combination-inhibition ability of original antibody to be index, for example whether have the concentration dependent inhibitory action with antigenic the combination, estimate its activity with regard to described monoclonal antibody.

In more detail, cultivate the zooblast of the expression vector conversion of the DNA that uses the antibody that comprises code book invention change, for example COS7 cell or Chinese hamster ovary celI.Cultured cells and/or culture medium supernatant or the antibody of the change of purification therefrom are used for measuring and combine with antigenic.Compare with the culture medium supernatant of cultivating the cell that only transforms expression vector.For antigen, for example antibody MABL-1 and antibody MABL-2, the specimen of the antibody that the present invention is changed or the mouse leukemia L1210 cell line that the contrast supernatant adds expressing human IAP are for example carried out flow cytometry then and are measured, to estimate antigen-binding activity.

Utilize following method ExternalEstimate signal transduction effect (the apoptosis induction effect of antagonist MABL-1 and antibody MABL-2): the specimen of the antibody of above-mentioned change is added the cell of expressing this antibody or changed in the cell of this antibody gene, utilize conventional method, estimate by changing due to the signal transduction, for example whether with people IAP-antigenic specificity mode inducing cell death.

Utilize following method In the bodyEstimate the apoptosis induction effect, for example under the situation of the antibody recognition people IAP that changes (for example the antibody sources of Gai Bianing is in antibody MABL-1 and antibody MABL-2): the mouse model of preparation human myeloma.To giving the antibody of monoclonal antibody or change of the present invention in the mouse vein, induce to have the nucleated blood cell apoptosis of IAP.Control group mice only gives PBS.Based on the variation of human IgG content and time-to-live thereof in the mice serum, estimate its apoptosis-induced effect with Graft Versus Tumor.

As mentioned above, comprise two or more H chain V districts and two or more L chain V districts and specificity antibody in conjunction with the change of molecule in target cell surface molecular or the cell by preparation, and by above-mentioned In the bodyOr ExternalEstimate the antibody of this change of screening, can obtain the antibody of change of the present invention.

The present invention comprises two or more H chain V districts and two or more L chains V district, preferred every kind of 2-4, more preferably 2 every kind the antibody of change, may be the dimerization that comprises the strand Fv in a H chain V district and a L chain V district, or the single chain polypeptide that wherein has two or more H chains V district to link to each other with two or more L chains V district.Can think, because such structure makes peptide simulate the three dimensional structure of natural aglucon, thereby keeps good antigen binding characteristic and agonist activity.

The antibody of change of the present invention has significantly reduced molecular size than antibody molecule (fully IgG), thereby has the high-permeability to tissue or tumor, than primary agonist monoclonal antibody high activity more.Therefore, suitably select the parental antibody various signals of might transduceing to enter cell, and the various effects of inducing cell, for example apoptosis-induced, inducing cell propagation, inducing cell differentiation, inducing cell division or Cycle Regulation effect.The pharmaceutical preparation that comprises them is used for the treatment of the disease that can cure by the inducement signal transduction, for example cancer, inflammation, hormone disturbance, autoimmune disease and hematopathy, for example leukemia, malignant lymphoma, aplastic anemia, osteomyelodysplasia syndrome and polycythemia vera.Also expect to can be used as contrast agent after antibody of the present invention is through the RI labelling.By connecting RI chemical compound or toxin, can strengthen its effect.

Optimum mode of operation of the present invention

The present invention will specifically illustrate according to following examples, but not limit the scope of the invention.

For illustrating the preparation method of the antibody that the present invention changes, the embodiment of preparation strand Fv is as follows.Use mouse anti human IAP antibody among the embodiment of the antibody that preparation changes, MABL-1 and MABL-2.On JIUYUE 11st, 1997, the hybridoma MABL-1 and the MABL-2 of these two antibody will be produced, be preserved in microbial preservation mechanism internationally with FERM BP-6100 and FERM BP-6101 respectively---state-run life sciences and human technical research institute, industrial science and technology institute, Ministry of International Trade and Industry (National Institute of Bioscience andhuman Technology, Agency of Industrial Science and Technology, Minister of International Trade and Industry) (1-3Higasi 1-Chome, Tsukuba-shi, Ibaraki-Ken, Japan).

Embodiment

Embodiment 1 (coding DNA in clone's mouse anti human IAP monoclonal antibody V district)

The coding DNA of the variable region of following clone's mouse anti human IAP monoclonal antibody MABL-1 and MABL-2.

1.1 preparation messenger RNA (mRNA)

Utilize mRNA Purification test kit (Pharmacia Biotech) to obtain the mRNA of hybridoma MABL-1 and MABL-2.

1.2 synthetic double chain cDNA

Utilize Marathon cDNA Amplification test kit (CLONTECH) from about 1 μ gmRNA synthetic double chain cDNA, and connect a joint.

1.3PCR the coding group of amplification antibody variable region

Utilize Thermal Cycler (PERKIN ELMER) to carry out PCR.

(1) amplification MABL-1L chain V district encoding gene

The primer that is used for PCR method is an Adapter primer-1 shown in the SEQ ID No.1, (CLONTECH), the partial sequence hybridization of it and joint, and MKC shown in the SEQ ID No.2 (Mus Kappa is constant) primer (Bio/Technology, 9,88-89,1991), hybridize in it and mice Kappa type L chain V district.

50 μ l PCR solution comprise 5 μ l, 10 * PCR buffer II, 2mM MgCl ₂, 0.16mMdNTPs (dATP, dGTP, dCTP and dTTP), the 2.5 archaeal dna polymerase AmpliTaqGold of unit (PERKIN ELMER), 0.2 μ M SEQ ID No.1 joint primer, 0.2 μ MSEQ ID No.2MKC primer and 0.1 μ g derive from the double-stranded cDNA of MABL-1.Solution is in 94 ℃ of preheatings of initial temperature 9 minutes, 1 minute, 60 ℃ of 94 ℃ of heating 1 minute successively then, 72 ℃ 1 minute 20 seconds.This temperature cycles repeats 35 times, and 72 ℃ of reheat reactant mixtures are 10 minutes then.

(2) code cDNA in amplification MABL-1H chain V district

MHC-γ 1 (murine heavy chain is constant) primer (Bio/Technology, 9,88-89,1991) shown in Adapter primer-1 shown in the SEQ ID No.1 and the SEQ ID No.3 is as the PCR primer.

Amplification method according to the described L chain of embodiment 1.3-(1) V district gene carries out the cDNA amplification, only is to use 0.2 μ M MHC-γ, 1 primer, but not 0.2 μ M MKC primer.

(3) code cDNA in amplification MABL-2L chain V district

The MKC primer of the Adapter primer-1 of SEQ ID No.1 and SEQ ID No.2 is as the PCR primer.

Carry out the cDNA amplification according to the amplification method of the described MABL-1L chain of embodiment 1.3-(1) V district gene, only be to use 0.1 μ g to derive from the double-stranded cDNA of MABL-2, but not the double-stranded cDNA of 0.1 μ g MABL-1.

(4) code cDNA in amplification MABL-2H chain V district

MHC-γ 2a primer (Bio/Technology, 9,88-89,1991) shown in the Adapter primer-1 of SEQ ID No.1 and the SEQ ID No.4 is as the PCR primer.

Carry out the cDNA amplification according to the described L chain of embodiment 1.3-(3) V district gene amplification method, only be to use 0.2 μ M MHC-γ 2a primer, but not 0.2 μ M MKC primer.

1.4 purified pcr product

Utilize the dna fragmentation of the above-mentioned pcr amplification of QIAquick PCR Purifcation test kit (QIAGEN) purification, and be dissolved in the 10mM Tris-HCl (pH8.0) that contains 1mM EDTA.

1.5 connect and conversion

Above-mentioned preparation comprise the about 140ng of dna fragmentation that comes from Kappa type mice MABL-1L chain V district encoding gene, be connected 3 hours with 50ng pGEM-T Easy carrier (Promega) in 15 ℃, reaction buffer comprises 30mM Tris-HCl (pH7.8), 10mMMgCl ₂, 10mM dithiothreitol, DTT, 1mM ATP and 3 T4DNA of unit ligases (Promega).

Then 1 μ l reactant mixture is added 50 μ l E.ColiIn the DH5 α competent cell (Toyobo Inc.), cell is in preserving 30 minutes on ice, and 42 ℃ of incubations 1 minute were preserved 2 minutes on ice again.Add 100 μ l SOC culture medium (GIBCO BRL).Will E.coliCell inoculation is (Molecular Cloning:A laboratory Manual in the LB agar culture medium that contains 100 μ g/ml ampicillin (SIGMA), people such as Sambrook, ColdSpring Harbor laboratory Press, 1989), 37 ℃ of overnight incubation obtain E.coliTransformant.

Transformant contains in the LB culture medium of 50 μ g/ml ampicillin at 3ml, and 37 ℃ of overnight incubation use QIAprep Spin Minprep test kit (QIAGEN) to prepare plasmid DNA from culture.

Gained comprises the plasmid of the encoding gene in the mice Kappa type L chain V district that comes from hybridoma MABL-1, called after pGEM-M1L.

According to above-mentioned identical method, from the dna fragmentation of purification, prepare plasmid, it comprises the mice H chain V district encoding gene that comes from hybridoma MABL-1, called after pGEM-M1H.

Prepare plasmid from the dna fragmentation of purification, it comprises the mice Kappa type L chain V district encoding gene that comes from hybridoma MABL-2, called after pGEM-M2L.

Prepare plasmid from the dna fragmentation of purification, it comprises the mice H chain V district encoding gene that comes from hybridoma MABL-2, called after pGEM-M2H.

Embodiment 2 (dna sequencing)

Utilize Auto DNA Sequencer (Applied Biosystem) and ABI PRISMDye Terminator Cycle Sequencing Ready Reaction test kit (AppliedBiosystem), according to manufacturer's rules, measure the nucleotide sequence of cDNA coding region in the above-mentioned plasmid.

Comprise the L chain V district encoding gene of mouse antibodies MABL-1 among the plasmid pGEM-M1L, its nucleotide sequence is shown in SEQ ID No.5.

Comprise the H chain V district encoding gene of mouse antibodies MABL-1 among the plasmid pGEM-M1H, its nucleotide sequence is shown in SEQ ID No.6.

Comprise the L chain V district encoding gene of mouse antibodies MABL-2 among the plasmid pGEM-M2L, its nucleotide sequence is shown in SEQ ID No.7.

Comprise the H chain V district encoding gene of mouse antibodies MABL-2 among the plasmid pGEM-M2H, its nucleotide sequence is shown in SEQ ID No.8.

Embodiment 3 (determining CDR)

The common structural similarity in V district of L chain and H chain, its per four framework regions are by three hypervariable regions, and promptly complementary determining region (CDR) links to each other.The aminoacid sequence of framework is relatively very conservative, and the aminoacid sequence of CDR alterable height (people such as Kabat E.A. extremely, " Sequences ofProteins of Immunological Interest ", US Dept.Health and HumanServices, 1983).

Based on these facts, with the variable region amino acid sequence of mouse anti human IAP monoclonal antibody, be applied in the antibody amino acid sequence database that people such as Kabat sets up, retrieve its homology.

Table 1 is depicted as the CDR district that determines according to homology.

Table 1

Plasmid	SEQ ID No.	CDR(1)	CDR(2)	CDR(3)
					pGEM-M1L pGEM-M1H pGEM-M2L pGEM-M2H	5 6 7 8	43-58 50-54 43-58 50-54	74-80 69-85 74-80 69-85	113-121 118-125 113-121 118-125

Embodiment 4 (identifying expression, the chimeric MABL-1 antibody of preparation and the chimeric MABL-2 antibody of clone cDNA)

4.1 the carrier of chimeric MABL-1 antibody is expressed in preparation

The cDNA clone pGEM-M1L and the pGEM-M1H in the L chain of encoding murine antibody MABL-1 and H chain V district after the PCR method change, introduce HEF expression vector (WO92/19759) respectively, and the carrier of chimeric MABL-1 antibody is expressed in preparation.

The forward primer MLS (SEQ ID No.9) in design L chain V district and the forward primer MHS (SEQ ID No.10) in H chain V district, make it and to hybridize with the coding DNA that each V district targeting sequencing begins to locate, and comprise Kozak consensus sequence (J.Mol.Biol., 196,947-950,1987) and HindIII Restriction Enzyme site.Design L chain V district reverse primer MLAS (SEQ ID No.11) and H chain V district reverse primer MHAS (SEQ ID No.12) make it and can hybridize with the terminal coding DNA in J district, and comprise donor splicing site sequence and BamHI Restriction Enzyme site.

To comprise 10 μ l, 10 * PCR buffer II, 2mM MgCl ₂, 0.16mM dNTPs (dATP, dGTP, dCTP and dTTP), 100 μ l PCR solution of the 5 archaeal dna polymerase AmpliTaq Gold of unit, every kind of primer of 0.4uM and 8ng template DNA (pGEM-M1L or pGEM-M1H), 94 ℃ of preheatings of initial temperature 9 minutes, then successively 94 ℃ the heating 1 minute, 60 ℃ 1 minute, 72 ℃ 1 minute 20 seconds.This temperature cycles repeats 35 times, 72 ℃ of reheat reactant mixtures 10 minutes.

With QIAquick PCR Purification test kit (QIAGEN) purified pcr product, then with HindIII and BamHI digestion.The product cloning in L chain V district in HEF expression vector HEF-K, with the product cloning in H chain V district in HEF expression vector HEF-γ.Behind the dna sequencing, comprise the segmental plasmid of correct sequence DNA, respectively called after HEF-M1L and HEF-M1H.

4.2 the carrier of chimeric MABL-2 antibody is expressed in preparation

According to identical method as described in embodiment 4.1, cDNA is changed and clones, only being to use pGEM-M2L and pGEM-M2H is template DNA, but not pGEM-M1L and pGEM-M1H.Behind the dna sequencing, contain the segmental plasmid of correct sequence DNA, respectively called after HEF-M2L and HEF-M2H.

4.3 rotaring redyeing COS 7 cell

In the COS7 cell, test above-mentioned expression vector, observe the transient expression of chimeric MABL-1 and MABL-2 antibody.

(1) The gene of the chimeric MABL-1 antibody of transfection

Use Gene Pulser device (BioRad), by electroporation with HEF-M1L and HEF-M1H carrier cotransformation COS7 cell.Adding every kind of DNA (10 μ g) and 0.8ml contain 1 * 10 in the cuvette ⁷The PBS of cell/ml.Mixture is handled with 1.5KV, 25 μ F capacitor pulses.

Room temperature was recovered after 10 minutes, electroporation of cells was forwarded in the DMFM culture medium of the hyclone that comprises 10% no gamma globulin (GIBCO BRL).Cultivate after 72 hours, collect supernatant, centrifugal removal cell debris also reclaims.

(2) The encode gene of chimeric MABL-2 antibody of transfection

Utilize as same procedure as described in the embodiment 4.3-(1), the encoding gene cotransfection COS7 cell with chimeric MABL-2 antibody only is to use HEF-M2L and HEF-M2H carrier, but not HEF-M1L and HEF-M1H carrier.In kind reclaim supernatant.

4.4 flow cytometry

Utilize above-mentioned COS7 cells and supernatant to carry out flow cytometry, measure its antigen combination.With expressing the COS7 cell of chimeric MABL-1 antibody or expressing the culture supernatant or the human IgG antibody in contrast (SIGMA) of cell of the COS7 of chimeric MABL-2 antibody, add 4 * 10 ⁵In the L-1210 L1210 cell of expressing human IAP, ice bath.After the washing, to the anti-human IgG antibody (Cappel) who wherein adds the FITC labelling.After incubation and the washing, (BECTON DICKINSON) measures its fluorescence intensity with the FACScan device.

Because the L1210 cell of chimeric MABL-1 and MABL-2 antibody specificity associative list intelligent IAP can confirm that these chimeric antibodys have the correct V plot structure (accompanying drawing 1-3) of mouse monoclonal antibody MABL-1 and MABL-2 respectively.

Embodiment 5 (the reconstruction strand Fv (scFv) of preparation antibody MABL-1 and antibody MABL-2)

5.1 the reconstruction strand Fv of preparation antibody MABL-1

Be prepared as follows the reconstruction strand Fv of antibody MABL-1.Utilize PCR method increase respectively H chain V district and the L chain V district of antibody MABL-1, and joint, and link together, with the reconstruction strand Fv of preparation antibody MABL-1.Preparation method as shown in Figure 4.Utilize the strand Fv of six kinds of primers (A-F) preparation antibody MABL-1.Primer A, C and E have just sequence, and primer B, D and F have antisense sequences.

The forward primer VHS in H chain V district (primer A, SEQ ID No.13) is designed to and can hybridizes with H chain V district N end coding DNA, and comprises NcoI Restriction Enzyme recognition site.The reverse primer VHAS in H chain V district (primer B, SEQ ID No.14) is designed to and can hybridizes with H chain V district C end coding DNA, and overlapping with joint.

The forward primer LS of joint (primer C, SEQ ID No.15) is designed to and can hybridizes with joint N end coding DNA, and overlapping with H chain V district C end coding DNA.The reverse primer LAS of joint (primer D, SEQ ID No.16) is designed to and can hybridizes with joint C end coding DNA, and overlapping with L chain V district N end coding DNA.

The forward primer VLS in L chain V district (primer E, SEQ ID No.17) is designed to and can hybridizes with joint C end coding DNA, and overlapping with L chain V district N end coding DNA.Reverse primer VLAS-FLAG (the primers F in L chain V district, SEQ ID No.18) is designed to and hybridizes with L chain V district C end coding DNA, and has a FLAG peptide-coding sequence (people such as Hopp T.P., Bio/Technology, 6,1204-1210,1988), two termination codon and EcoRI Restriction Enzyme recognition site.

In first PCR step, carry out A-B, C-D, three reactions of E-F, its PCR product of purification.Three kinds of PCR products that first step PCR obtains are assembled according to its complementarity.Add primer A and F then, the total length coding DNA of the reconstruction strand Fv of amplification antibody MABL-1 (the second step PCR).The plasmid that is used separately as template among the first step PCR comprises, the plasmid pGEM-M1H (referring to embodiment 2) in encoding antibody MABL-1H chain V district, (this connector area comprises: (people such as Huston J.S. Gly GlyGly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser (SEQ ID No.19)) to comprise the plasmid pSC-DP1 of connector area DNA sequences encoding, Proc.Natl Acad.Sci.USA, 85,5879-5883,1988), and the plasmid pGEM-M1L (referring to embodiment 2) in encoding antibody MABL-1L chain V district.

50 μ l solution of first step PCR reaction comprise 5 μ l10 * PCR buffer II, 2mMMgCl ₂, 0.16mM dNTPs, the 2.5 archaeal dna polymerase AmpliTaq Gold of unit (PERKIN ELMER), every kind of primer of 0.4 μ M and every kind of template DNA of 5ng.PCR solution is 94 ℃ of preheatings of initial temperature 9 minutes, 1 minute, 65 ℃ of 94 ℃ of heating 1 minute successively then, 72 ℃ 1 minute 20 seconds.This temperature cycles repeats 35 times, and 72 ℃ of reheat reactant mixtures are 7 minutes then.

Utilize QIApuick PCR Purification test kit (QIAGEN) purified pcr product A-B (371bp), C-D (63bp) and E-F (384bp), and in the second step PCR, assemble.Among the second step PCR, will comprise 120ng first step PCR product A-B, 20ng PCR product C-D and 120ng PCR product E-F, 10 μ l, 10 * PCR buffer II, 2mMMgCl ₂, 0.16mM dNTPs, the 5 archaeal dna polymerase AmpliTaq Gold of unit (PERKINELMER) 98 μ l PCR solution, 94 ℃ of preheatings of initial temperature 8 minutes, then successively 94 ℃ the heating 2 minutes, 65 ℃ 2 minutes, 72 ℃ 2 minutes.This temperature cycles repeats 2 times, adds every kind of primer A and F 0.4 μ M respectively in the reaction then.Mixture is 94 ℃ of preheatings of initial temperature 1 minute, then 1 minute, 65 ℃ 1 minute, 72 ℃ of 94 ℃ of heating 1 minute and 20 seconds successively.This temperature cycles repeats 35 times, and 72 ℃ of reheat reactant mixtures are 7 minutes then.

The 843bp dna fragmentation of second step of purification PCR preparation is with NcoI and EcoRI digestion.The dna fragmentation that obtains is cloned into the pSCFVT7 carrier.Expression vector pSCFVT7 comprises and is suitable for E.coliThe pelB signal sequence of periplasmic expression system (people such as Lei S.P., J.Bacteriology, 169,4379-4383,1987).Behind the dna sequencing, will comprise the segmental plasmid of coding DNA of the correct aminoacid sequence of reconstruction strand Fv of antibody MABL-1, called after " pscM1 " (referring to Fig. 5).Comprise nucleotide sequence and aminoacid sequence that antibody MABL-1 rebuilds strand Fv among the plasmid pscM1, shown in SEQ ID No.20.

Utilize PCR method to change the pscM1 carrier, rebuild the carrier of strand Fv with preparation expressing antibodies MABL-1 in mammalian cell.The dna fragmentation that obtains is introduced the pCHO1 expression vector.Expression vector pCHO1 is through EcoRI and SmaI digestion DHFR-Δ E-rvH-PM1-f (WO92/19759), remove antibody gene, and connects EcoRI-NotI-BamHI adapter (Takara ShuZo) and make up.

Sal-VHS primer shown in the design SEQ ID No.21, as the PCR forward primer, it can be hybridized with the coding DNA of H chain V district N end, and comprises Sal I Restriction Enzyme recognition site.Design is the FRH1anti primer shown in SEQ ID No.22, and as the PCR reverse primer, it can be hybridized with the coding DNA of the first frame sequence end.

To comprise 10 μ l, 10 * PCR buffer II, 2mM MgCl ₂, 0.16mM dNTPs, the 5 archaeal dna polymerase AmpliTaq Gold of unit, every kind of primer of 0.4 μ l and 8ng template DNA (pscM1) 100 μ l PCR solution, 95 ℃ of preheatings of initial temperature 9 minutes, then successively 95 ℃ the heating 1 minute, 60 ℃ 1 minute, 72 ℃ 1 minute 20 seconds.Repeat this temperature cycles 35 times, 72 ℃ of reheat reactant mixtures are 7 minutes then.

Utilize QIAquick PCR Purifcation test kit (QIAGEN) purified pcr product,, obtain the N end coding DNA fragment of the reconstruction strand Fv of antibody MABL-1 with SalI and MboII digestion.With MboII and EcoRI digestion pscM1 carrier, obtain the reconstruction strand Fv C end coding DNA fragment of antibody MABL-1.This SalI-MboII dna fragmentation and MboII-EcoRI dna fragmentation are cloned into the pCH01-IgS carrier.Behind the dna sequencing, will comprise the plasmid called after " pCHOM1 " (referring to Fig. 6) of target DNA sequence.Expression vector pCHO1-Igs comprises the mice IgG1 signal sequence (Nature, 322,323-327,1988) that is suitable for mammalian cell secreting, expressing system.The nucleotide sequence of the antibody MABL-1 reconstruction strand Fv that plasmid pCHOM1 comprises and aminoacid sequence are shown in SEQ ID No.23.

5.2 the reconstruction strand Fv of preparation antibody MABL-2

According to the foregoing description 5.1 methods, the reconstruction strand Fv of preparation antibody MABL-2.Utilize the plasmid pGEM-M2H (referring to embodiment 2) in the H chain V district of coding MABL-2 among the first step PCR, but not pGEM-M1H, and the plasmid pGEM-M2L (referring to embodiment 2) in the L chain V district of coding MABL-2, but not pGEM-M1L, thereby obtain plasmid pscM2, the coding DNA fragment of the strand Fv purpose aminoacid sequence of the antibody MABL-2 that wherein comprises.Comprise the reconstruction strand Fv of antibody MABL-2 among the plasmid pscM2, its nucleotide sequence and aminoacid sequence are shown in SEQ ID No.24.

Utilize PCR method to change the pscM2 carrier, preparation carrier pCHOM2 is used for expressing at mammalian cell, wherein comprises the coding DNA fragment of the correct aminoacid sequence of reconstruction strand Fv of antibody MABL-2.The nucleotide sequence of the reconstruction strand Fv of the antibody MABL-2 that comprises among the plasmid pCHOM2 and aminoacid sequence are shown in SEQ ID No.25.

5.3 rotaring redyeing COS 7 cell

Test pCHOM2 carrier in the COS7 cell, the transient expression of the reconstruction strand Fv of observation antibody MABL-2.

Utilize Gene Pulser device (BioRad), pCHOM2 is transformed the COS7 cell by electroporation.In cuvette, add DNA (10 μ g) and 0.8ml and contain 1 * 10 ⁷The PBS of cell/ml.With 1.5KV, 25 μ F capacitor pulse treatment mixture.

Room temperature is recovered electroporation of cells to be forwarded to (GIBCO BRL) in the IMDM culture medium that contains 10% hyclone after 10 minutes.Cultivate after 72 hours, collect supernatant, centrifugal removal cell debris also reclaims.

5.4 in the COS7 cells and supernatant, detect the reconstruction strand Fv of antibody MABL-2

In the culture supernatant of the COS7 cell of pCHOM2 carrier transfection, confirm the existence of the strand Fv of antibody MABL-2 by Western trace method.

Will be through the culture supernatant of the COS7 cell of pCHOM2 carrier transfection, and compare through the COS7 of pCHO1 transfection cells and supernatant, carry out the SDS electrophoresis, and forward (Schleicher ﹠amp on the REINFORCED NC film to; Schuell).With 5% skim milk (Morinaga Nyu-gyo) closing membrane,, mix (SIGMA) with anti-FLAG antibody with the 0.05%Tween20-PBS washing.Film is incubation, washing and the link coupled mouse IgG antibody of mixed-alkali phosphatase (Zymed) at room temperature.After incubation and the washing, add substrate solution (Kirkegaard Perry Laboratories) colour developing (Fig. 7) under the room temperature.

Only in the culture supernatant of the COS7 cell of introducing the pCHOM2 carrier, detect FLAG peptide specific protein, thereby the reconstruction strand Fv of proof antibody MABL-2 is secreted in the culture supernatant.

5.5 flow cytometry

Utilize above-mentioned COS7 cells and supernatant, measure the antigen combination by flow cytometry.With the COS7 cells and supernatant of the reconstruction strand Fv of expressing antibodies MABL-2, the COS7 cells and supernatant that transforms through the pCHO1 carrier that perhaps compares adds 2 * 10 ⁵In the L-1210 L1210 cell of individual expressing human integrin associated protein matter (IAP), perhaps add in the cell of the L1210 cell line that the pCOS1 compare transforms.After ice bath and the washing, add mouse anti FLAG antibody (SIGMA).Incubation cell and washing then.To the anti-mouse IgG antibody (BECTONDICKINSON) that wherein adds the FITC labelling, the incubation cell also washs once more afterwards.Measure fluorescence intensity with FACScan device (BECTON DICKINSON) subsequently.

Because the strand Fv of antibody MABL-2 combines with the L1210 cell-specific of expressing human IAP, so can determine the reconstruction strand Fv of antibody MABL-2 people's integrin associated protein matter (IAP) is had affinity (referring to Fig. 8-11).

5.6 competitive ELISA

According to inhibition activity, measure the combination activity of the reconstruction strand Fv of antibody MABL-2 to the mouse monoclonal antibody conjugated antigen.

To resist FLAG antibody to be adjusted into 1 μ g/ml, add in every hole of 96 orifice plates, 37 ℃ of incubations 2 hours.After the washing, seal with 1%BSA-PBS.After incubation and the washing, will introduce the COS7 cells and supernatant of secreting type people IAP antigen gene (SEQ ID No.26) under the room temperature, be diluted to two volumes, add in every hole with PBS.Under the room temperature after incubation and the washing, 50 μ l are adjusted into the mixture of COS7 cells and supernatant of reconstruction strand Fv of the expressing antibodies MABL-2 of the biotinylation MABL-2 antibody of 100ng/ml and 50 μ l serial dilutions, add in every hole.After incubation and the washing, add the link coupled streptavidin of alkali phosphatase (Zymed) in every hole under the room temperature.After incubation and the washing, add substrate solution (SIGMA) under the room temperature, measure in every hole reactant mixture at the absorbance at 405nm place.

The result shows, compares with the COS7 cells and supernatant of introducing pCHO1, and by contrast, reconstruction strand Fv (MABL2-scFv) the concentration dependent ground of antibody MABL-2 significantly suppresses mouse antibodies MABL-2 and combines (Figure 12) with people IAP is antigenic.Therefore prompting, among the reconstruction strand Fv of antibody MABL-2, the structure in each V district that comes from mouse monoclonal antibody MABL-2 is all correct.

5.7 external apoptosis induction effect

The L1210 cell of end user IAP gene transfection, L1210 cell and the CCRF-CEM cell that compare through the transfection of pCOS1 carrier, by Annexin-V dyeing (Boehninger Mannheim), detect the apoptosis-induced effect of the reconstruction strand Fv of antibody MABL-2.

1 * 10 ⁵In above-mentioned every kind of cell, add the COS7 cells and supernatant of reconstruction strand Fv of expressing antibodies MABL-2 or the COS7 cells and supernatant through the transfection of pCHO1 carrier that compares by 50% final concentration, mixture was cultivated 24 hours.Carry out Annexin-V dyeing then, measure fluorescence intensity with FACScan device (BECTON DICKINSON).

The coloration result of Annexin-V is respectively shown in Figure 13-18.The point in lower-left district is represented living cells, and the point in bottom right district is represented the early stage cell of apoptosis, and the point in upper right district is represented apoptosis cell in late period.The result shows, the reconstruction strand Fv (MABL2-scFv) of antibody MABL-2 has induced specificity at the antigenic L1210 cell death of people IAP (Figure 13-16) significantly, compared with the control, the strand Fv of reconstruction also significantly induces CCRF-CEM cell death (Figure 17-18).

5.8 in Chinese hamster ovary celI, express the strand Fv that comes from MABL-2

With pCHOM2 carrier transfection CHO cell, set up the Chinese hamster ovary celI system of the strand Fv (polypeptide) that constant expression comes from antibody MABL-2.

Use Gene Dulser device (BioRad), the pCHOM2 carrier is transformed Chinese hamster ovary celI by electroporation method.Add DNA (10 μ g) and 0.7ml in the cuvette and contain Chinese hamster ovary celI (1 * 10 ⁷The mixture of the PBS of cell/ml).With 1.5KV, 25 μ F capacitor pulse treatment mixture.Room temperature was recovered after 10 minutes, electroporation of cells is forwarded in the free nucleic acid α-MEM culture medium (GIBCO BRL) that contains 10% hyclone cultivate.The expression of destination protein matter among the clone who confirms to obtain with SDS-PAGE is selected the high clone of expression, as the production cell line of the strand Fv that comes from antibody MABL-2.In containing the serum-free medium CHO-S-SFM II (GIBCO BRL) of 10nM methotrexate (SIGMA), cultivate this cell line.Then, collect culture supernatant, centrifugal removal cell debris also reclaims.

5.9 the strand Fv that comes from MABL-2 that produces in the purifying CHO cell

With the culture supernatant of the Chinese hamster ovary celI system of the expression strand Fv that obtains among the embodiment 5.8, utilize hemodialysis's post (PAN130SF, ASAHI MEDICALS) to concentrate nearly 20 times.Concentrated solution-20 ℃ preservation, and when purification, melt.

Utilize three kinds of chromatography methods, i.e. Blue-sepharose, hydroxyapatite and gel filtration, purification of single stranded Fv from the Chinese hamster ovary celI culture supernatant.

(1) The Blue-sepharose column chromatography

With 20mM acetate buffer (pH6.0) spissated supernatant is diluted 10 times, centrifugal removal insoluble matter (10000 * rev/min, 30 minutes).With same buffer balance Blue-sepharose post (20ml), with sample on the supernatant.After washing post with same buffer, utilize in the same buffer progressively gradient 0.1,0.2,0.3,0.5 to the NaCl up to 1.0M, the protein that adsorbs in the eluting post.Cross component and each elution fraction with the SDS-PAGE analysis stream.With confirming that the component (0.1-0.3M NaCl elution fraction) that contains strand Fv mixes, utilize Centri Prep-10 (AMICON) to concentrate up to about 20 times.

(2) Hydroxyapatite

(1) concentrated solution that obtains in dilutes 10 times with 10mM phosphate buffer (pH7.0), and last sample hydroxyapatite column (20ml, BIORAD).(pH7.0) washes post with the 60ml10mM phosphate buffer.Then, use linear gradient, the protein that adsorbs in the eluting post (referring to Figure 19) up to the sodium phosphate buffer of 200mM.Analyze each component with SDS-PAGE, confirm in component A and B component, to exist strand Fv.

(3) Gel filtration

Concentrate component A and B in (2) respectively with CentriPrep-10, and go up sample to using the equilibrated TSKgelG3000SWG post (21.5 * 600mm) of 20mM acetate buffer (pH6.0) that contains 0.15M NaCl.Tomographic map as shown in figure 20.Analyze each component through SDS-PAGE, confirmed that two main peaks (AI and BI) are purpose strand Fv.During gel filtration was analyzed, component A was at 36KDa apparent molecular weight place eluting, and B component is at 76kDa place eluting.Analyze the strand Fv (AI, BI) of purification with the 15%SDS polyacrylamide gel.Having or not having and handle sample in the presence of the Reducing agent, carry out electrophoresis according to Laemmli ' s method.Use coomassie brilliant blue staining protein then.As shown in figure 21, no matter whether Reducing agent exists, and AI and BI present the strand at 35kDa apparent molecular weight place.Can infer that from the above AI is the monomer of strand Fv, BI is the non-covalent bonded dimer of strand Fv.(7.5 * 60mm) by gel filtration analysis component AI and BI, shows only to detect the monomer peak in component AI, only detects dimer peak (Figure 22) in B component I to use the TSKgelG3000SW post.Dimer component (B component I) accounts for 4 percent of total strand Fv.Dimer above 90% in the dimer component can 4 ℃ of stable preservations above 1 month.

5.10 be structured in the carrier of expressing the strand Fv that comes from antibody MABL-2 in the E.Coli cell

Utilize PCR method to change the pscM2 carrier, exist with preparation E.coliThe carrier of the strand Fv of effective expression antibody MABL-2 in the cell.The dna fragmentation that obtains is introduced the pSCFVT7 expression vector.

Design is the Nde-VHSm02 primer shown in SEQ ID No.27, and as the PCR forward primer, it can be hybridized with H chain V district N end coding DNA, and comprises start codon and NdeI Restriction Enzyme recognition site.Design is the VLAS primer shown in SEQ ID No.28, and as the PCR reverse primer, it can be hybridized with L chain V district C end coding DNA, and comprises two termination codoies and EcoRI Restriction Enzyme recognition site.For E.coliMiddle effective expression comprises 5 point mutation in forward primer Nde-VHSm02 with in the interfertile part of H chain V district N end coding DNA.

To comprise 10 μ l10 * PCR buffer #1,1mM MgCl ₂, 0.2mM dNTPs, 5 unit K OD archaeal dna polymerases (all from TOYOBO), every kind of primer of 1 μ M and 100ng template DNA (pscM2) 100 μ lPCR solution, 15 seconds, 65 ℃ of 98 ℃ of heating 2 seconds and 74 ℃ are 30 seconds successively.Repeat this temperature cycles 25 times.

Utilize QIAquick PCR Purifcation test kit (QIAGEN) purified pcr product, with NdeI and EcoRI digestion, the dna fragmentation that obtains is cloned into the pSCFVT7 carrier then, has removed pel B signal sequence with NdeI and EcoRI digestion in this carrier.Behind dna sequencing, with the plasmid called after " pscM2DEm02 " (referring to Figure 23) that comprises the dna fragmentation that has the target DNA sequence that produces.The nucleotide sequence of the strand Fv that comes from antibody MABL-2 that comprises among the plasmid pscM2DEm02 and aminoacid sequence are shown in SEQ IDNo.29.

5.11 in the E.coli cell, express the strand Fv that comes from antibody MABL-2

Transform with the pscM2DEm02 carrier E.coliBL21 (DE3) pLysS (STRATAGENE) obtains expressing the strand Fv's that comes from antibody MABL-2 E.coliBacterial strain.Check the expression of destination protein matter among the clone who obtains with SDS-PAGE, select the high clone of expression, as the production bacterial strain of the strand Fv that comes from antibody MABL-2.

5.2 the strand Fv that comes from antibody MABL-2 that produces among the purification E.coli

Conversion is obtained E.coliMonoclonal was cultivated 7 hours for 28 ℃, then 28 ℃ of overnight incubation in 70ml LB culture medium in 3ml LB culture medium.Pre-culture is transferred in the 7L LB culture medium, and 300 rev/mins of stirrings are with 28 ℃ of cultivations of Jar fermentation tank.When the culture medium absorbance reaches O.D.=1.5, induce antibacterial, cultivated then 3 hours with 1mM IPTG.

Culture medium centrifugal (10000 * g, 10 minutes) reclaims the bacterial precipitation thing.In antibacterial, add the 50mM Tris-HCl buffer (pH8.0) that contains 5mM EDTA, 0.1M NaCl and 1%Triton x-100, the ultrasonic disruption antibacterial (output: 4, cycle of operation: 70%, 1 minute * 10 times).The suspension of centrifugal broken antibacterial (12000g, 10 minutes) is with the precipitation inclusion body.Isolating inclusion body is mixed with the 50mM Tris-HCl buffer (pH8.0) that contains 5mM EDTA, 0.1M NaCl and 4%Tritonx-100, supersound process (output: 4 once more, cycle of operation: 50%, 30 seconds * 2 times), centrifugal (12000g, 10 minutes) separate destination protein matter precipitation, remove the protein that comprises in the supernatant.

Cracking contains the inclusion body of destination protein matter in containing the 50mM Tris-HCl buffer (pH8.0) of 6M urea, 5mM EDTA and 0.1M NaCl, last sample is to Sephacryl S-300 solvent resistant column (5 * 90cm, Amersharm Pharmacia), this post 50mM Tris-HCl buffer (pH8.0) balance that contains 4M urea, 5mM EDTA, 0.1M NaCl and 10mM mercaptoethanol, flow velocity is 5ml/ minute, to remove relevant high molecular strand Fv.Analyze the component that obtains through SDS-PAGE, highly purified protein component is diluted to O.D with the used buffer of gel filtration ₂₈₀=0.25.With the 50mMTris-HCl buffer (pH8.0) that contains 5mM EDTA, 0.1M NaCl, 0.5MArg, 2mM reduced glutathione and 0.2mM oxidized form glutathione, this component of dialysing 3 times is so that protein refolding then.Further with dialyse this component three times of the 20mM acetate buffer (pH6.0) that contains 0.15M NaCl, with exchange buffering liquid.

To dialyse sample on the product to Superdex 200pg solvent resistant column (2.6 * 60cm.Amersharm Pharmacia), this post is with 20mM acetate buffer (pH6.0) balance that contains 0.15M NaCl, and is a small amount of by the crosslinked high molecular weight protein of intermolecular S-S key to remove.As shown in figure 24, go out two peaks, i.e. main peak and submaximum at broad peak (be contemplated to high molecular and assemble thing) back eluting.SDS-PAGE analysis (referring to Figure 21) and gel filtration are analyzed the eluting position at these two peaks, and the prompting main peak is a strand Fv monomer, the covalently bound strand Fv of submaximum right and wrong dimer.Non-covalent bonded dimer accounts for 4% of total strand Fv.

5.13 come from the external apoptosis induction activity of the strand Fv of antibody MABL-2

Utilize to introduce the L1210 cell (hIAP/L1210) of people IAP gene, according to two kinds of Annexin-V dyeing (Boehringer Mannheim) methods, detect Chinese hamster ovary celI and E.coliThe apoptosis-induced effect of the strand Fv (MABL2-scFv) that comes from antibody MABL-2 that produces.

In the first method, to 5 * 10 ⁴Add sample antibody in the individual hIAP/L1210 cell, final concentration is 3 μ g/ml, cultivates 24 hours.Analytic sample antibody, monomer and the dimer that promptly obtain among the embodiment 5.9 from the MABL-2 strand Fv of Chinese hamster ovary celI, among the embodiment 5.12 from E.coliMonomer and the dimer of the MABL-2 strand Fv that obtains, and the mouse IgG antibody that compares.Carry out Annexin-V dyeing after the cultivation, (BECTON DICKINSON) measures its fluorescence intensity with the FACScan device.

In the second method, to 5 * 10 ⁴Add sample antibody in the individual hIAP/L1210 cell, final concentration is 3 μ g/ml, cultivates 2 hours, and mixes with anti-FLAG antibody (SIGMA), and final concentration is 15 μ g/ml, cultivates 22 hours again.Analyzing samples antibody, they are the monomers from the MABL-2 strand Fv of the Chinese hamster ovary celI acquisition of embodiment 5.9, and the mouse IgG antibody that compares.Carry out Annexin-V dyeing after the cultivation, use its fluorescence intensity of FACScan measurement device.

The result of Annexin-V staining analysis is shown in Figure 25-31.The result shows, Chinese hamster ovary celI and E.coliIn the dimer of the MABL-2 strand Fv polypeptide that produces, (Figure 25) compared with the control, remarkable inducing cell death (Figure 26,27), and at Chinese hamster ovary celI with E.coliThe monomer of the middle MABL-2 strand Fv polypeptide that produces is not then observed apoptosis-induced effect (Figure 28,29).When using anti-FLAG antibody together, (Figure 30) compared with the control, the remarkable inducing cell death of monomer (Figure 31) of the strand Fv polypeptide that comes from MABL-2 antibody that produces in the Chinese hamster ovary celI.

5.14scFv/CHO the monomer of polypeptide and dimer are imitated the antitumor of human myeloma model mice Should

(1) human IgG in the quantitative measurement mice serum

The human IgG (M protein) that utilizes following ELISA method to measure to contain in the mice serum, produce by human myeloma cell.(BIOSOURCE Lot#7902) is diluted to 1 μ g/ml, and every hole adds 100 μ l in 96 orifice plates (Nunc), and 4 ℃ are incubated overnight, make antibody immobilization with the anti-human IgG antibody of goat with 0.1% bicarbonate buffer (pH9.6).After the sealing, add mice serum that 100 μ l progressively dilute or (CAPPEL, Lot#00915), incubation is 2 hours under the room temperature as the human IgG of standard to every hole.Xian Dihou, (BIOSOURCE, Lot#6202), incubation is 1 hour under the room temperature to add the anti-human IgG antibody of the alkali phosphatase enzyme mark of 5000 times of dilutions of 10 μ l.The washing back adds substrate solution.Behind the incubation, use MICROPLATEREADER Model 3550 (BioRad) to measure the absorbance at 405nm place.Based on the calibration curve that the light absorption value by the human IgG standard substance obtains, calculate the concentration of human IgG in the mice serum.

(2) Preparation administrable antibody

On administration same day, the monomer and the dimer of scFv/CHO polypeptide is diluted to 0.4mg/ml or 0.25mg/ml respectively, preparation administrable sample with the PBS (-) of aseptic filtration.

(3) The mouse model of preparation human myeloma

Be prepared as follows the mouse model of human myeloma.To be suspended in the RPMI1640 culture medium that contains 10% hyclone (GIBCO-BRL) (GIBCO-BRL) at the KPMM2 cell (JP-Appl.7-236475) of SCID mice (Japan Clare) interior generation, be adjusted into 3 * 10 ⁷Cell/ml.By the tail vein with 200 μ l KPMM2 cell suspension (6 * 10 ⁶Cell/mice) transplant to SCID mice (male, 6 ages in week), this SCID mice takes off sialic acid GM1 antibody (WAKO JUNYAKU, 1 bottle is dissolved in 5ml) in transplanting subcutaneous injection the previous day.

(4) Antibody administration

With the antibody sample of preparation in (2), monomer (250 μ l) and dimer (400 μ l) are administered intravenously in the human myeloma cell model mice of preparation in (3) by tail.Transplant the KPMM2 cell and begin administration after 3 days, every day 2 times, carried out 3 days.In contrast, by the PBS (-) of tail intravenously administrable 200gl aseptic filtration, one day 2 times, carried out 3 days equally.Every group comprises 7 mices.

(5) Estimate the monomer and dimeric anti-the swelling of scFv/CHO polypeptide with the human myeloma model mice The tumor effect

According to (M protein) variation of concentration of human IgG in the mice serum and the time-to-live of mice, estimate scFv/CHO polypeptide monomer and dimeric Graft Versus Tumor with the human myeloma model mice.Collect mice serum in 24 days behind the transplanting KPMM2 cell, utilize the variation of the ELISA method mensuration human IgG concentration in above-mentioned (1).The amount of human IgG (M protein) is brought up to about 8500 μ g/ml in PBS (-) administration group (contrast) serum, and the amount of the human IgG of scFv/CHO dimer administration group is obviously lower, is equal to or less than 1/10th of matched group.So this result shows scFv/CHO dimer strong inhibition KPMM2 cell growth (Figure 32).As shown in figure 33, compare, obviously prolong in the observed time-to-live of scFv/CHO dimer administration group with PBS (-) administration group.

Can confirm that as mentioned above the scFv/CHO dimer has Graft Versus Tumor for the human myeloma model mice.Can think that scFv/CHO dimer, the i.e. antibody of change of the present invention, its Graft Versus Tumor are because the antibody of this change has apoptosis-induced effect.

5.15 hemagglutination reaction experiment

Publish according to Tokyo Kagaku Dojin, the Zoku-Seikagaku Jikken Koza " Immuno-Biochemical Investigation " that Japanese biochemical society edits carries out the hemagglutination reaction test and determines hemagglutination reaction.

Get healthy donor blood with the syringe of handling through heparin,, use the red cell suspension of PBS (-) preparation final concentration 2% then with PBS (-) washing three times.Test sample book comprises, MABL-2 antibody, and the monomer and the dimer of the strand Fv polypeptide that Chinese hamster ovary celI produces, E.coliThe monomer and the dimer of the strand Fv polypeptide that produces are contrast (ZYMED) with mice IgG.Utilization is available from the round bottom 96 orifice plates research hemagglutination effect of Falcon.Add the above-mentioned antibody sample of 50 μ l in every hole, and 50 μ l, 2% red cell suspension, Yu Kongzhong mixes.37 ℃ of incubations after 2 hours spend the night 4 ℃ of preservations of reactant mixture, measure its hemagglutination reaction.Contrast is every hole 50 μ l PBS (-), by carry out the hemagglutination reaction test with quadrat method.Mice IgG that uses and the antibody final concentration of antibody MABL-2 are 0.01,0.1,1.0,10.0 or 100.0 μ g/ml.The strand Fv final concentration that uses is 0.004,0.04,0.4,4.0,40.0 or 80.0 μ g/ml, only exists E.coliUnder the polypeptide dimer situation that produces, use 160.0 μ g/ml in addition.The result is as shown in table 2.For antibody MABL-2, under greater than 0.1 μ g/ml concentration, observe hemagglutination reaction, and monomer and the dimer of strand Fv are not all observed hemagglutination reaction.

Table 2 hemagglutination reaction test

	Contrast	0.01	0.1	1	10	100	μg/ml
										MIgG MABL-2 (complete)	- -	- -	- +	- +++	- +++	- ++
	Contrast	0.004	0.04	0.4	4	40	80	μg/ml
										ScFv/CHO monomer scFv/CHO dimer	- -	- -	- -	- -	- -	- -	- -
	Contrast	0.004	0.04	0.4	4	40	80	160	μg/ml
										ScFv/E.coli monomer scFv/E.coli dimer	- -	- -	- -	- -	- -	- -	- -	-

Embodiment 6 comprises the antibody sc (Fv) of the change in 2 H chain V districts and 2 L chain V districts ₂, and the antibody MABL-2scFv with different length joint.

6.1 make up antibody MABL-2sc (Fv) ₂Expression plasmid

Utilize following PCR method to change above-mentioned pCHOM2, wherein comprise the coding DNA of the scFv that comes from above-mentioned MABL-2, the dna fragmentation that produces is inserted pCHOM2, with the antibody [sc (Fv) of preparation change ₂] expression plasmid of (wherein comprising two H chain V districts and two L chain V districts of coming from antibody MABL-2).

The PCR the primer comprises, EF1 primer (SEQ 2D No:30) is a forward primer, be designed to and hybridize with EF1 α coding DNA, and reverse primer (SEQ ID No:19), be designed to and hybridize with L chain V district C end coding DNA, and comprise the DNA sequences encoding of connector area and the VLLAS primer (SEQ ID No:31) that comprises SalI Restriction Enzyme recognition site.

100 μ l PCR solution comprise 10 μ l 10x PCR buffer #1,1mM MgCl ₂, 0.2mM dNTPs (dATP, dGIP, dCTP and dTTP), 5 unit K OD archaeal dna polymerases (Toyobo, Inc.), every kind of primer of 1 μ M and 100ng template DNA (pCHOM2).With this PCR solution 94 ℃ of heating 30 seconds, 50 ℃ 30 seconds and 74 ℃ successively 1 minute.Repeat this temperature cycles 30 times.

Utilize QIAquick PCR Purification test kit (QIAGEN) purified pcr product, and digest with SalI.The dna fragmentation that produces is cloned into pBluescript KS ⁺Carrier (Toyobo, Inc.).Behind the dna sequencing, digest the plasmid that comprises the target DNA sequence with SalI, the dna fragmentation that obtains is connected with the pCHOM2 that digests through SalI with rapid DNA Ligation test kit (BOEHRINGER MANNHEIM).Behind the dna sequencing, will comprise the plasmid called after " pCHOM2 (Fv) of target DNA sequence ₂" (referring to Figure 34).Plasmid pCHOM2 (Fv) ₂In the antibody MABL-2sc (Fv) that comprises ₂The nucleotide sequence in district and aminoacid sequence are shown in SEQ ID No.32.

6.2 preparation has the expression plasmid of the antibody MABL-2scFv of different length joint.

H chain and L chain encoding cDNA that utilization comes from MABL-2 make template, and preparation scFv as described below wherein comprises different length joint and V district, by [H chain]-[L chain] (hereinafter claiming " HL ") or [L chain]-[H chain] (hereinafter claiming " LH ") order design.

Utilize pCHOM2 (Fv) ₂Make template, PCR method makes up HL type scFv.In the PCR step, use primer to CFHL-F1 (SEQ ID No:33) and CFHL-R2 (SEQID No:34), or primer is to CFHL-F2 (SEQ ID No:35) and CFHL-R1 (SEQID No:36) and KOD polymerase.Carry out the PCR program, temperature cycles is 94 ℃ 30 seconds, 60 ℃ 30 seconds and 72 ℃ 1 minute, repeats 30 times, and producing the H chain cDNA that 5 ' end contains targeting sequencing, or its 3 ' end contains the L chain cDNA of FLAG sequence.Mix H chain and the L chain cDNA produced, carry out PCR, temperature cycles is 94 ℃ 30 seconds, 60 ℃ 30 seconds and 72 ℃ successively 1 minute, repeats 5 times, uses mixture to be template and KOD polymerase.In reactant mixture, add CFHL-F1 and CFHL-R1 primer, repeat said temperature circulation 30 times then, carry out the PCR reaction, to produce jointless HL-0 type cDNA.

For making up LH type scFv, utilize pGEM-M2L and pGEM-M2H to be template, wherein comprise the code cDNA in L chain V district and the H chain V district of antibody MABL-2 respectively, carry out PCR reaction (referring to JP-Appl.11-63557).Use primer to T7 (SEQ IDNo:37) and CFLH-R2 (SEQ ID No:38), or primer is to CFLH-F2 (SEQID No:39) and CFLH-R1 (SEQ ID No:40), and KOD polymerase (ToyoboInc.).Temperature cycles is 94 ℃ 30 seconds, 60 ℃ 30 seconds and 72 ℃ in turn 1 minute, repeats 30 times, carries out the PCR reaction, and producing the L chain cDNA that 5 ' end comprises targeting sequencing, or its 3 ' end comprises the H chain cDNA of FLAG sequence.Mixing L chain and the H chain cDNA produced, is template with this mixture, uses the KOD polymerase, and temperature cycles comprises successively 94 ℃ 30 seconds, 60 ℃ 30 seconds and 72 ℃ 1 minute, repeats 5 times, carries out PCR.Add T7 and CFLH-R1 primer in the reactant mixture, the said temperature circulation repeats 30 times and reacts.With the product is template, utilizes primer to CFLH-F4 (SEQ ID No:41) and CFLH-R1, and temperature cycles comprises successively 94 ℃ 30 seconds, 60 ℃ 30 seconds and 72 ℃ 1 minute, repeats 30 times, carries out PCR, to produce jointless LH-0 type cDNA.

LH-0 that produces and HL-0 type cDNA introduce the cDNA that digests respectively among the mammal expression plasmid INPEP4 with Ligation High (Toyobo Inc.) with EcoRI and BamHI Restriction Enzyme (Takara Shuzo) digestion.With each plasmid transformed competence colibacillus E.coliJM109 (Nippon Gene) utilizes QIAGEN Plasmid Maxi test kit (QUIAGEN) from transforming E.coliThe middle purpose plasmid that separates.Thereby preparation plasmid pCF2LH-0 and pCF2HL-0.

The HL type expression plasmid that comprises different banjo connectors for structure, with pCF2HL-0 is template, the sense primer that uses is CFHL-X3 (SEQ ID No:42), CFHL-X4 (SEQ IDNo:43), CFHL-X5 (SEQ ID No:44), CFHL-X6 (SEQ ID No:45) or CFHL-X7 (SEQ ID No:46), antisense primer is BGH-1 (SEQ ID No:47), itself and the complementation of carrier sequence.Use the KOD polymerase to carry out the PCR reaction, temperature cycles comprises successively 94 ℃ 30 seconds, 60 ℃ 30 seconds and 72 ℃ 1 minute, repeats usefulness Restriction Enzyme XhoI and BamHI (Takara Shuzo) digestion reaction product 30 times.The fragment of digestion uses Ligation High (Toyobo Inc.) to introduce between the XhoI and BamHI site of pCF2HL-0 respectively.With each plasmid transformed competence colibacillus E.coliJM109 utilizes Qiagen Plasmid Maxi test kit from transforming E.coliThe middle purpose plasmid that separates.Thereby preparation expression plasmid pCF2HL-3, pCF2HL-4, pCF2HL-5, pCF2HL-6 and pCF2HL-7.

For being structured in the expression plasmid of transient expression in the COS7 cell, with Restriction Enzyme EcoRI and BamHI (Takara Shuzo) digested plasmid pCF2HL-0, pCF2HL-3, pCF2HL-4, pCF2HL-5, pCF2HL-6 and pCF2HL-7, with about 800bp fragment of agarose gel electrophoresis purification generation.Utilize Ligation High (Toyobo Inc.) respectively the fragment that obtains to be introduced between the EcoRI and BamHI site of expression plasmid pCOS1, be used for expressing at mammalian cell.With each plasmid transformed competence colibacillus E.coliDH5 α (ToyoboInc.) uses Qiagen Plasmid Maxi test kit from transforming E.coliThe middle purpose plasmid that separates.Thereby preparation expression plasmid CF2HL-0/pCOS1, CF2HL-3/pCOS1, CF2HL-4/pCOS1, CF2HL-5/pCOS1, CF2HL-6/pCOS1 and CF2HL-7/pCOS1.

As the representative instance of these plasmids, the structure of plasmid CF2HL-0/pCOS1 as shown in figure 35, the MABL2-scFv＜HL-0 that comprises in this plasmid〉nucleotide sequence and aminoacid sequence shown in SEQ ID No.48.The nucleotide sequence in these plasmid center tap districts and aminoacid sequence are also as shown in figure 36.

The LH type expression plasmid that comprises different banjo connectors for structure, use pCF2LH-0 to be template, CFLH-X3 (SEQ ID No:49), CFLH-X4 (SEQ ID No:50), CFLH-X5 (SEQ ID No:51), CFLH-X6 (SEQ ID No:52) or CFLH-X7 (SEQ ID No:53) are sense primer, BGH-1 is an antisense primer, itself and the complementation of carrier sequence.Utilize the KOD polymerase, temperature cycles comprises successively 94 ℃ 30 seconds, 60 ℃ 30 seconds and 72 ℃ 1 minute, repeats 30 times, carries out the PCR reaction, and product is with Restriction Enzyme XhoI and BamHI digestion.Utilize Ligation High respectively the fragment of digestion to be introduced between the XhoI and BamHI site of pCF2LH-0.Each plasmid transformed competence colibacillus E.coliDH5 α (Toyobo Inc.) uses Qiagen Plasmid Maxi test kit to separate the purpose plasmid from the E.coli that transforms.Thereby preparation expression plasmid pCF2LH-3, pCF2LH-4, pCF2LH-5, pCF2LH-6 and pCF2LH-7.

For making up COS7 cell transient expression plasmid, plasmid pCF2LH-0, pCF2LH-3, pCF2LH-4, pCF2LH-5, pCF2LH-6 and pCF2LH-7 are with Restriction Enzyme EcoRI and BamHI (Takara Shuzo) digestion, with about 800bp fragment of agarose gel electrophoresis purification generation.Utilize Ligation High respectively the fragment that obtains to be introduced between the XhoI and BamHI site of mammalian cell expression plasmid pCOS1.With each plasmid transformed competence colibacillus E.coliDH-5 α (Toyobo Inc.) utilizes Qiagen Plasmid Maxi test kit from transforming E.coliThe middle purpose plasmid that separates.Thereby preparation expression plasmid CF2LH-0/pCOS1, CF2LH-3/pCOS1, CF2LH-4/pCOS1, CF2LH-5/pCOS1, CF2LH-6/pCOS1 and CF2LH-7/pCOS1.

As the representative instance of these plasmids, the structure of plasmid CF2LH-0/pCOS1 as shown in figure 37, the MABL2-scFv＜LH-0 that comprises in this plasmid〉nucleotide sequence and aminoacid sequence shown in SEQ ID No.54.The nucleotide sequence in these plasmid center tap districts and aminoacid sequence are also as shown in figure 38.

6.3 in the COS7 cell, express scFv and sc (Fv) ₂

(1) prepares culture supernatant with containing blood serum medium

The scFv and the sc (Fv) of (JCRB9127, Japan Health Sciences Foundation) transient expression HL type and LH type in the COS7 cell ₂At CO ₂In the gas incubator, with the DMEM culture medium (GIBCO BRL) that contains 10% hyclone (HyClone), 37 ℃ of cultivation COS7 cells that go down to posterity.Use Gene Pulser device (BioRad), by CF2HL-0,3～7/pCOS1 or CF2LH-0, the 3～7/pCOS1 of electroporation with embodiment 6.2 preparations, or pCHOM2 (Fv) ₂The carrier rotaring redyeing COS 7 cell.Add DNA (10 μ g) and 0.25ml in the cuvette and contain 2 * 10 ⁷The DMEM culture medium of cell/ml, 10%FBS and 5mM BES (SIGMA).After keeping 10 minutes, with 0.17kv, 950 μ F capacitor pulse treatment mixture.Room temperature is recovered to forward electroporation of cells to 75cm after 10 minutes ³In the DMEM culture medium (10%FBS) of culture bottle.Cultivate after 72 hours, collect culture supernatant, centrifugal removal cell debris.Culture supernatant is filtered with 0.22um filter (FALCON), to obtain culture supernatant (hereinafter claiming " CM ").

(2) prepare culture supernatant with serum-free medium

Will be by forwarding 75cm to (1) same procedure cells transfected ³Overnight incubation in the DMEM culture medium (10%FBS) of culture bottle.After the cultivation, supernatant discarded is used the PBS washed cell, adds CHO-S-SFM II culture medium (GIBCO BRL) then.Cultivate after 72 hours, collect culture supernatant, centrifugal removal cell debris filters to obtain CM with 0.22um filter (FALCON).

6.4 detect scFv and sc (Fv) among the COS7CM ₂

With various MABL2-scFv and the sc (Fv) among the COS7CM of preparation in Western trace method detection the foregoing description 6.3 (2) ₂

The CM of each COS7 of SDS-PAGE electrophoretic analysis forwards REINFORCED NC film (Schleicher ﹠amp to; Schuell) on.With 5% skim milk (Morinaga Nyu-gyo) closing membrane, the TBS washing.Then to wherein adding anti-FLAG antibody.Film is incubation and washing at room temperature.The mouse IgG antibody (Jachson Immuno Research) that adds peroxidase labelling.After incubation and the washing, add substrate solution (KirKegaard Perry Laboratories) colour developing (Figure 39) under the room temperature.

6.5 flow cytometry

Use the COS7 cells and supernatant of embodiment 6.3 (1) preparations, carry out flow cytometry, measure MABL2-scFv and sc (Fv) ₂With antigenic combination of people's integrin associated protein matter (IAP).With culture supernatant to be measured or the COS7 cells and supernatant that compares, add 2 * 10 ⁵The mouse leukemia cell L1210 of individual expressing human IAP.After ice bath and the washing, add 10 μ g/ml mouse anti FALG antibody (SIGMA), incubation and washed cells then.The anti-mouse IgG antibody (BECTON DICKINSON) that adds the FITC labelling then, incubation and washed cell once more.Utilize FACScan device (BECTON DICKINSON) to measure fluorescence intensity.The flow cytometry result shows in the COS7 cells and supernatant, to have the MABL2-scFv and the sc (Fv) of different length joint ₂People IAP had high affinity (referring to Figure 40 a and 40b).

6.6 external apoptosis induction effect

By Annexin-V dyeing (Boehringer Mannheim), utilize the L1210 cell (hIAP/L1210) of transfection people IAP gene, detect the apoptosis-induced effect of the COS7 culture supernatant of embodiment 6.3 (1) preparations.

To 5 * 10 ⁴Add COS7 cells and supernatant in the individual hIAP/L1210 cell with each carrier transfection, or the COS7 cells and supernatant that compares, final concentration is 10%, mixture was cultivated 24 hours.Carry out Annexin-V/PI dyeing then, utilize FACScan device (BEKTON DICHINSON) to measure fluorescence intensity.The result shows, the scFv＜HL3,4,6,7 among the COS7 cell CM, LH3,4,6,7〉and sc (Fv) ₂Significantly induced the death of hIAP/L1210 cell.These results as shown in figure 41.

Be used for the carrier that Chinese hamster ovary celI is expressed scFv and sc (Fv) 2 6.7 make up

Be separation, purification MABL2-scFv and sc (Fv) 2 from culture supernatant, following structure is used for the carrier that Chinese hamster ovary celI is expressed.

Use Ligation High,, introduce between the EcoRI and BamHI site of expressing cho cell carrier pCHO1 the pCF2HL-0 of embodiment 6.2 preparations, 3～7 and pCF2LH-0,3～7 EcoRI-BamHI fragment.Use its transformed competence colibacillus E.coliDH5 α.Use QIAGEN Plasmind Midi test kit (QIAGEN) from transforming E.coliIn separation quality grain, preparation expression plasmid pCHOM2HL-0,3～7 and pCHOM2LH-0,3～7.

6.8 MABL2-scFv＜HL-0,3～7 is expressed in preparation 〉, MABL2-scFv＜LH-0,3～7 and sc (Fv) ₂Chinese hamster ovary celI, and prepare its culture supernatant

Each expression plasmid pCHOM2HL-0 that makes up with embodiment 6.7,3～7 and pCHOM2LH-0,3～7, and pCHOM2 (Fv) ₂Carrier transforms Chinese hamster ovary celI, with the Chinese hamster ovary celI of the antibody for preparing every kind of change of constant expression.As its representative instance, constant expression MABL2-scFv＜HL-5〉or sc (Fv) ₂Being prepared as follows of Chinese hamster ovary celI shown in.

By Restriction Enzyme PvuI digestion, linearisation expression plasmid pCHOM2HL-5 and pCHOM2 (Fv) ₂, use Gene Pulser device (BioRad) by the electroporation transfection Chinese hamster ovary celI.In cuvette, add DNA (10 μ g) and 0.75ml 1 * 10 ⁷The PBS of cell/ml handles with 1.5KV, 25 μ F capacitor pulses.Room temperature was recovered after 10 minutes, electroporation of cells was forwarded to (GIBCO BRL) cultivation in the α that the comprises nucleic acid-MEM culture medium that contains 10% hyclone.After the overnight incubation, supernatant discarded.Use the PBS washed cell, add the free nucleic acid α-MEM culture medium (GIBCO BRL) that contains 10% hyclone.After cultivating for two weeks, cell being cultivated in the culture medium that contains 10nM (final concentration) methotrexate (SIGMA), is the methotrexate of 50nM and 100nM then.The cell that obtains is at CHO-S-SFM II serum-free medium (GIBCO BRL) transfer flask culture.Collect culture supernatant, centrifugal removal cell debris with 0.22 μ m aperture membrane filtration, obtains CM respectively.

As mentioned above, obtained constant expression MABL2-scFv＜HL-0 ,-3 ,-4 ,-6 ,-7〉and＜LH-0 ,-3 ,-4 ,-5 ,-6 ,-7 Chinese hamster ovary celI and CM thereof.

6.9 purification MABL2-scFv＜HL-5〉dimer and sc (Fv) ₂

Utilize following two kinds of purification process, purification MABL2-scFv＜HL-5 from the CM of embodiment 6.8 preparations〉and sc (Fv) ₂

＜purification process 1 〉

Utilization is positioned at the FLAG sequence of peptide C end, by anti-FLAG antibody affinity column chromatography and gel filtration purification HL-5 and sc (Fv) ₂With anti-FLAG M2 affinity gel (SIGMA) preparation pillar (7.9ml), with the 50mM Tris-HCl buffer (TBS, pH7.5) balance, the 1 liter of CM that last sample obtains in 6.8 that contain 150mM NaCl.After washing post with TBS, by 0.1M glycine-HCl buffer (pH3.5) eluting scFv.Utilize SDS-PAGE to analyze resulting component, the scFv that confirmed eluting.The scFv component can be mixed up to 0.01% Tween 20 with final concentration, and concentrate with Centricon-10 (MILIPORE).(7.5 * 600mm) with after containing 20mM acetate buffer (pH6.0) balance of 150mM NaCl and 0.01%Tween 20, with sample on the concentrated solution for TSKgel G3000SWG post.Flow velocity is the 0.4ml/ branch, by the absorbance detection scFv of 280nm.Key component at dimer place eluting is HL-5, the eluting sc of monomer place (Fv) ₂

＜purification process 2 〉

Use ion-exchange chromatography, hydroxyapatite and gel filtration, three step purification HL-5 and sc (Fv) ₂In ion-exchange chromatography, purification HL-5 uses Q Sepharose Fast Flow post (Pharmacia), purification sc (Fv) ₂Use SP-sepharose Fast Flow post.After among second step, reaching, handle HL-5 and sc (Fv) with same program ₂

The first step of HL-5

The CM of HL-5 transfers pH to 9.0 with 1M Tris then with 2 times of 20mM Tris-HCl buffer (pH9.0) dilutions that contains 0.02%Tween 20.Behind 20mM Tris-HCl buffer (pH8.5) the balance Q sepharose fast flow post that contains 0.02%Tween20, with sample on the solution.By containing the same buffer of 0.1-0.55M NaCl linear gradient, bonded polypeptide on the eluting post.Through SDS-PAGE monitoring elution fraction, collected the elution fraction that contains HL-5, on the hydroxyapatite in last sample to the second step.

Sc (Fv) ₂The first step

Sc (Fv) ₂CM with 2 times of 20mM acetate buffer (pH5.5) dilutions that contains 0.02%Tween 20, transfer pH to 5.5 with 1M acetic acid.Behind 20mM acetate buffer (pH5.5) the balance SP-sepharose fast flow post that contains 0.02%Tween 20, with sample on the solution.By bonded polypeptide on the buffer solution elution post that contains 0～0.5M NaCl linear gradient.Through SDS-PAGE monitoring elution fraction, collection comprises sc (Fv) ₂Component, in the hydroxyapatite in last sample to the second step.

Second stepHL-5 and sc (Fv) ₂Hydroxyapatite chromatography

(the I type BIORAD) with after containing 10mM phosphate buffer (pH7.0) balance of 0.02%Tween 20, goes up HL-5 and sc (Fv) that the sample first step obtains respectively to hydroxyapatite column ₂Component.After washing post with same buffer, utilize phosphate buffer, bonded polypeptide on the eluting post up to the 0.5M linear gradient.Through the component of SDS-PAGE monitoring eluting, collect the component that comprises desired polypeptides.

The 3rd stepHL-5 and sc (Fv) ₂Gel filtration

Concentrate each component that second step obtained respectively with CentriPrep-10 (MILIPORE), and go up sample to usefulness contain 0.02%Tween 20 and 0.15M NaCl equilibrated Superdex 200 posts of 20mM acetate buffer (pH6.0) (2.6 * 60cm, Pharmacia).The place is eluted to HL-5 at dimer, sc (Fv) HL-5 and sc (Fv) ₂Respectively at the monomer place as main peaks by eluting.

Two kinds of purification process all are difficult to detect the HL-5 monomer, and when having confirmed that joint as strand Fv comprises about 5 aminoacid, high yield forms strand Fv dimer.In addition, the dimer of HL-5 and sc (Fv) ₂Can be behind purification 4 ℃ of stable preservations one month.

6.10 estimate the scFv＜HL-5 of purification〉dimer and sc (Fv) ₂With the antigenic activity that combines

Utilize the MABL2-scFv＜HL-5 of purification〉sc (Fv) of dimer and purification ₂Carry out flow cytometry, to estimate itself and antigenic combination of people's integrin associated protein matter (IAP).MABL2-scFv＜HL-5 with 10 μ g/ml purification〉sc (Fv) of dimer, purification ₂, positive control antibody MABL-2 or negative control mice IgG (Zymed), add 2 * 10 ⁵The mouse leukemia L1210 cell (hIAP/L1210) of individual expressing human IAP, or the L1210 cell (pCOS1/L1210) that transforms through pCOS1 compares.After ice bath and the washing, add 10 μ g/ml mouse anti FLAG antibody (SIGMA), incubation and washed cells then.To the anti-mouse IgG antibody that wherein adds the FITC labelling (BECTION DICKINSON), incubation and washed cell once more.Utilize FACScan device (BECTON DICKINSON) to measure fluorescence intensity then.

Because the MABL2-scFv＜HL-5 of purification〉sc (Fv) of dimer and purification ₂Specificity can confirm scFv＜HL-5 in conjunction with the hIAP/L1210 cell〉dimer and sc (Fv) ₂People IAP had high-affinity (referring to Figure 42).

6.11 the scFv＜HL-5 of purification〉dimerization and sc (Fv) ₂In external apoptosis induction activity

Use L1210 cell (hIAP/L1210) and the human leukemia cell line CCRF-CEM cell of introducing people IAP gene, by Annexin-V dyeing (BoehringerMannheim), detect the MABL2-scFv＜HL-5 of purification〉sc (Fv) of dimer and purification ₂Apoptosis-induced effect.

MABL2-scF＜HL-5 with the purification of variable concentrations〉MABL2-sc (Fv) of dimer, purification ₂, positive control antibody MABL-2 or negative control mice IgG, add 5 * 10 ⁴Individual hIAP/L1210 cell or 1 * 10 ⁵In the individual CCRF-CEM cell.Cultivate and carry out Annexin-V dyeing after 24 hours, use FACScan device (BECTONDICKLNSON) to measure its fluorescence intensity.As a result, MABL2-scFv＜HL-5〉dimer and MABL2-sc (Fv) ₂Rely on mode with concentration and significantly induce hIAP/L1210 and CCRF-CEM cell death (referring to Figure 43).The result shows, compares MABL2-scFv＜HL-5 with original antibody MABL-2〉and MABL2-sc (Fv) ₂Has enhanced apoptosis-induced effectiveness.

6.12 the scFv＜HL-5 of purification〉dimer and sc (Fv) ₂Hemagglutination reaction test

According to embodiment 5.15 methods, use the scFv＜HL-5 of the purification of variable concentrations〉sc (Fv) of dimer and purification ₂, carry out the hemagglutination reaction test.

As positive control, observe hemagglutination reaction with antibody MABL-2, and to single-chain antibody MABL2-sc (Fv) ₂And MABL2-scFv＜HL-5〉all do not observe hemagglutination reaction.In addition, two kinds of buffer that antibody MABL-2 uses carry out hemagglutination reaction, do not have essential difference.These results are as shown in table 3.

Table 3

The hemagglutination reaction test

Diluent: PBS (μ g/ml)
																		Contrast	28.9	14.45	7.225	3.6125	1.8063	0.9031	0.4516	0.2258	0.1129	0.0564	0.0282	0.0141	0.0071	0.0035	0.0018
MABL2- sc(Fv) 2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
																		Contrast	28.0	14.0	7.0	3.5	1.75	0.875	0.4375	0.2188	0.1094	0.0547	0.0273	0.0137	0.0068	0.0034	0.0017
MABL2- sc(Fv) <HL5>	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
																		Contrast	80	40	20	10	5	2.5	1.25	0.625	0.3125	0.1563	0.0781	0.0391	0.0195	0.0098	0.0049
MABL2 (complete) mlgG	- -	+ -	+ -	+ -	+ -	+ -	+ -	+ -	+ -	+ -	± -	- -	- -	- -	- -	- -
																	Diluent: acetate (μ g/ml)
	Contrast	80	40	20	10	5	2.5	1.25	0.625	0.3125	0.1563	0.0781	0.0391	0.0195	0.0098	0.0049
																	MABL2 (complete)	-	+	+	+	+	+	+	+	+	+	+	+	-	-	-	-

6.13 the scFv＜HL-5 of purification〉dimer and sc (Fv) ₂Graft Versus Tumor to the human myeloma model mice

ScFv＜the HL-5 of test implementation example 6.8 and 6.9 preparations and purification〉dimer and sc (Fv) ₂Graft Versus Tumor.The human myeloma mouse model that utilizes embodiment 5.1 to produce, ELISA measures the proteinic amount of M that human myeloma cell produces in the mice serum, and detects the time-to-live of mice, tests.According to the variation of the proteinic amount of M in the mice serum and the time-to-live of mice, estimate scFv＜HL-5 then〉dimer and sc (Fv) ₂Graft Versus Tumor.

Use 0.01,0.1 or HL-5 and the sc (Fv) of 1mg/ml in the test ₂, they are dissolved in the carrier that comprises 150mM NaCl, 0.02%Tween and 20mM acetate buffer (pH6.0), by 0.1,1 or 10mg/kg dosage to the mice administration.Control group mice only gives carrier.

Transplant human myeloma cell after 26 days, collect mice serum, press embodiment 5.14 methods, ELISA measures the proteinic amount of M in the serum.As a result, give HL-5 dimer and sc (Fv) ₂The proteinic amounts of serum M of two groups of mices, all with dose dependent mode descend (referring to Figure 44).In addition, compare, observe and give HL-5 (Figure 45) and sc (Fv) with the matched group that gives carrier ₂(Figure 46) two groups, equal significant prolongation of its time-to-live.These results show, HL-5 of the present invention and sc (Fv) ₂Have fabulous In the bodyGraft Versus Tumor.

Embodiment 7

Comprise the H chain V district of people's antibody 12B5 of anti-people MPL and the strand Fv in L chain V district

The encode V district of human monoclonal antibodies 12B5 of anti-people MPL of following structure:

7.1 make up the gene in coding 12B5H chain V district

Nucleotide sequence (SEQ ID NO:55) by its gene derives from human immunoglobulin gene (Eur.J.Immunol.1996 in 5 ' end connection; Targeting sequencing 26:63-69) (SEQ IDNO:56), the design coding is in conjunction with the gene in the H chain V district of people's antibody 12B5 of people MPL.The nucleotide sequence of design is divided into four oligonucleotide (12B5VH-1,12B5VH-2,12B5VH-3,12B5VH-4) that each has the 15bp overlapping sequence.Respectively, 12B5VH-1 (SEQ ID NO:57) and 12B5VH-3 (SEQ ID NO:59) are that just direction is synthetic, and 12B5VH-2 (SEQ ID NO:58) and 12B5VH-4 (SEQ ID NO:60) are synthetic for antisense orientation.After assembling each synthetic oligonucleotide according to complementarity separately, add outside primer (12B5VH-S and 12B5VH-A) amplification full-length gene.Respectively, 12B5VH-S (SEQ ID NO:61) is designed to 5 ' end hybridization by forward primer and targeting sequencing, and have Hind III Restriction Enzyme recognition site and a KoZak sequence, and 12B5VH-A (SEQID NO:62) is designed to by the nucleotide sequence hybridization of reverse primer with coding H chain V district C end, and has donor splicing site sequence and Bam HI Restriction Enzyme recognition site.

100 μ l PCR solution comprise 10 μ l 10x PCR Gold Buffer II, 1.5mMMgCl ₂, 0.08mM dNTPs (dATP, dGTP, dCTP and dTTP), the 5 archaeal dna polymerase AmpliTaq Gold of unit (all from PERKIN ELMER), every kind of synthetic oligonucleotide of 2.5p mol (12B5VH-1 is to-4), 94 ℃ of initial temperatures heating 9 minutes, 94 ℃ 2 minutes, 55 ℃ 2 minutes and 72 ℃ 2 minutes.After this circulation repeats 2 times, add outside primer 12B5VH-S of each 15pmol and 12B5VH-A.This mixture comprises 94 ℃ 30 seconds, 55 ℃ 30 seconds and 72 ℃ of circulations of 1 minute 35 times, and 72 ℃ of reheat 5 minutes.

Utilize 1.5% low melting point temperature agarose gel (Sigma) purified pcr product, utilize Restriction Enzyme BamHI and Hind III digestion, and be cloned into people H chain expression vector HEF-g γ 1.Behind the dna sequencing, will comprise the plasmid called after HEF-12B5H-g γ 1 of correct DNA sequence.

HEF-12B5H-g γ 1 usefulness Restriction Enzyme EcoRI and BamHI digestion, the gene of generation coding 12B5VH, it is cloned into human Fab H chain expression vector pCOS-Fd then, produces pFd-12B5H.Utilize pcr amplification to comprise to include the DNA (SEQ ID NO:63) of the encoding gene of subarea and people H chain part constant region, this intron district is positioned between the encoding gene of people's heavy chain of antibody V district and constant region, this PCR product is inserted animal cell expression carrier pCOS1, make up human Fab H chain expression vector.With HEF-g γ 1 is template, forward primer G1CH1-S (SEQ ID NO:64) (is designed to 5 ' the terminal sequence hybridization with introne 1, and have Restriction Enzyme recognition site EcoRI and a BamHI), reverse primer G1CH1-A (SEQID NO:65) (is designed to 3 ' end DNA hybridization with people H chain constant region CH1 domain, and have the sequence of a part of hinge region of coding, 2 termination codon and a Restriction Enzyme recognition site Bgl II), in above-mentioned the same terms amplification people H chain constant region gene down.

The 12B5H chain variable region that comprises reconstruction among plasmid HEF-12B5H-g γ 1 and the pFd-12B5H, its nucleotide sequence and aminoacid sequence are shown in SEQ ID NO:66.

7.2 make up the gene in coding 12B5L chain V district

Nucleotide sequence (SEQ ID NO:67) by gene derives from human immunoglobulin gene 3D6 (Nuc.Acid Res.1990:18 in 5 ' end connection; 4927) targeting sequencing (SEQ IDNO:68), the design coding is in conjunction with the gene in the L chain V district of people's antibody 12B5 of people MPL.Nucleotide sequence by above-mentioned same procedure design is divided into four oligonucleotide (12B5VL-1,12B5VL-2,12B5VL-3,12B5VL-4) that each has the 15bp overlapping sequence, synthesizes respectively.Respectively, 12B5VL-1 (SEQ ID NO:69) and 12B5VL-3 (SEQID NO:71) have just sequence, and 12B5VL-2 (SEQ ID NO:70) and 12B5VL-4 (SEQ ID NO:72) have antisense sequences.After assembling each synthetic oligonucleotide according to complementarity separately, (12B5VL-S and 12B5VL-A) mixes with outside primer, the amplification full-length gene.Respectively, 12B5VL-S (SEQ ID NO:73) is designed to 5 ' end hybridization by forward primer and targeting sequencing, and has Hind III Restriction Enzyme recognition site and Kozak sequence.12B5VL-A (SEQ ID NO:74) is designed to by the nucleotide sequence hybridization of reverse primer with coding L chain V district C end, and has donor splicing site sequence and Bam HI Restriction Enzyme recognition site.

Carry out PCR as mentioned above, utilize 1.5% low melting point temperature agarose gel (Sigma) purified pcr product, utilize Restriction Enzyme BamHI and Hind III digestion, and be cloned into people L chain expression vector HEF-g κ.Behind the dna sequencing, will comprise the plasmid called after HEF-12B5L-g κ of correct DNA sequence.Comprise the 12B5L chain V district of reconstruction among the plasmid HEF-12B5L-g κ, its nucleotide sequence and aminoacid sequence are shown in SEQ ID NO:75.

7.3 produce the 12B5 strand Fv (scFv) that rebuilds

The 12B5 antibody strand Fv that rebuilds is designed to 12B5VH-joint-12B5VL order, and the C end has FLAG sequence (SEQ ID NO:76), so that detect and purification.Utilization is by (Gly ₄Ser) ₃The joint sequence that 15 aminoacid of representative are formed makes up the 12B5 strand Fv (sc12B5) of this reconstruction.

(1) utilize the joint sequence of forming by 15 aminoacid to produce the 12B5 strand Fv that rebuilds

Utilize PCR increase respectively 12B5 H chain V district, connector area and 12B5 L chain V district and be connected, make up the encoding gene of the 12B5 antibody strand Fv of the reconstruction comprise the joint sequence of forming by 15 aminoacid.The method diagram is shown in Figure 47.The 12B5 strand Fv that utilizes six kinds of primers (A-F) preparation to rebuild.Primer A, C and E have just sequence, and primer B, D and F have antisense sequences.

The forward primer 12B5-S in H chain V district (primer A, SEQ ID No.77) is designed to and can hybridizes with 5 ' end of H chain targeting sequencing, and has EcoRI Restriction Enzyme recognition site.The reverse primer HuVHJ3 in H chain V district (primer B, SEQ ID No.78) is designed to and can hybridizes with the coding DNA of H chain V district C end.

The forward primer RHuJH3 of joint (primer C, SEQ ID No.79) is designed to the coding DNA hybridization with joint N end, and overlapping with the coding DNA of H chain V district C end.The reverse primer RHuVK1 of joint (primer D, SEQ ID No.80) is designed to and can hybridizes with the coding DNA of joint C end, and overlapping with the coding DNA of L chain V district N end.

The forward primer HuVK1.2 in L chain V district (primer E, SEQ ID No.81) is designed to the coding DNA hybridization with L chain V district N end.Reverse primer 12B5F-A (the primers F in L chain V district, SEQ ID No.82) is designed to and hybridizes with the coding DNA of L chain V district C end, and has a FLAG peptide-coding sequence (people such as Hopp T.P., Bio/Technology, 6,1204-1210,1988), two termination codon and NotI Restriction Enzyme recognition site.

In first PCR step, carry out A-B, C-D, three reactions of E-F, three kinds of PCR products that first step PCR is obtained are assembled according to complementarity separately.Add after primer A and the F, amplification has the total length coding DNA (the second step PCR) of 12B5 strand Fv joint, that rebuild that is made up of 15 aminoacid.Among the first step PCR respectively with the plasmid HEF-12B5H-g γ 1 (referring to embodiment 7.1) in the 12B5H chain V district of coding and rebuilding, plasmid pSCFVT7-hM21 (humanized ONS-M21 the antibody) (people such as Ohtomo who comprises connector area coding DNA (SEQ ID NO:83), Anticancer Res.18 (1998), 4311-4316) (this connector area is made up of Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser GlyGly Gly Gly Ser) (people such as Huston, Proc.Natl.Acad.Sci.USA, 85,5879-5883,1988), and the plasmid HEF-12B5L-g κ (referring to embodiment 7.2) in the 12B5L chain V district of coding and rebuilding is as template.

50 μ lPCR reaction solutions of the first step comprise 5 μ l, 10 * PCR Gold Buffer II, 1.5mM MgCl ₂, 0.08mM dNTPs, the 5 archaeal dna polymerase AmpliTaq Gold of unit (all from PERKIN ELMER), every kind of primer of 100pmol and every kind of template DNA of 100ng.PCR solution is 94 ℃ of initial temperatures heating 9 minutes, 94 ℃ 30 seconds, 55 ℃ 30 seconds, 72 ℃ 1 minute.This temperature cycles repeats after 35 times, 72 ℃ of reheat reactant mixtures 5 minutes.

Utilize second step assembling PCR product A-B, C-D and E-F.98 μ l, second step PCR mixture solution comprises 1 μ l first step PCR product A-B, 0.5 μ l PCR product C-D and 1 μ l

PCR product E-F is template, 10 μ l, 10 * PCR Gold Buffer II, 1.5mM MgCl ₂, 0.08mM dNTPs, the 5 archaeal dna polymerase AmpliTaq Gold of unit (all from PERKINELMER), 94 ℃ of initial temperatures heating 8 minutes, 94 ℃ 2 minutes, 65 ℃ 2 minutes, 72 ℃ 2 minutes.This circulation repeats after 2 times, adds each 100pmol. of every kind of primer A and F and repeats by after 94 ℃ 30 seconds, 55 ℃ 30 seconds, 72 ℃ circulations of forming in 1 minute 35 times, and reactant mixture was 72 ℃ of heating 5 minutes.

Utilize the dna fragmentation of 1.5% low melting point temperature agarose gel purification, second step PCR preparation,, be cloned into pCHO1 carrier and pCOS1 carrier (Japanese patent application No. 8-255196) with EcoRI and NotI digestion.Expression vector pCH01 utilizes EcoRI and SmaI digestion to remove antibody gene from DHFR-Δ E-rvH-PM1-f (referring to WO92/19759), and connects EcoRI-NotI-BamHI joint (TAKARA SHUZO) and make up.Behind the dna sequencing, will comprise the coding DNA segmental plasmid called after pCHO-sc12B5 and the pCOS-sc12B5 of the correct aminoacid sequence of 12B5 strand Fv of reconstruction.The nucleotide sequence of the 12B5 strand Fv of the reconstruction that comprises among plasmid pCHO-sc12B5 and the pCOS-sc12B5 and aminoacid sequence are shown in SEQ ID NO:84.

7.4 utilize animal cell expression antibody 12B5 (IgG, Fab) and strand Fv polypeptide

Utilize COS-7 cell or expressing cho cell antibody 12B5 (IgG, Fab) and derive from the strand Fv of antibody 12B5.

The following transient expression that utilizes the COS7 cell.Use Gene Pulser device (BioRad), carry out transfection by electroporation method.In being suspended in the COS-7 cell of 0.8ml PBS (1 * 10 ⁷Cell/ml), for expressing antibodies 12B5 (IgG) adds above-mentioned expression vector HEF-12B5H-g γ 1 and each 10 μ g of HEF-12B5L-g κ, add pFd-12B5H and each 10 μ g of HEF-12B5L-g κ for expressing the 12B5Fab fragment, add 10 μ g pCOS-sc12B5 for expressing strand Fv.Utilize 1.5KV, 25 μ FD capacitor pulses to handle the mixture in the cuvette.Room temperature was recovered after 10 minutes, electroporation of cells was added to (GIBCO BRL) cultivation in the DMEM culture medium that contains 10% hyclone.After the overnight incubation, with the PBS washed cell once, be added among the serum-free medium CHO-S-SFM II and cultivated 2 days.The centrifugal removal cell debris of culture medium, with 0.22 μ m membrane filtration, the preparation culture supernatant.

For setting up the Chinese hamster ovary celI system that stably express derives from the strand Fv (polypeptide) of antibody 12B5, following with expression vector pCHO-sc12B5 introducing Chinese hamster ovary celI.

Use Gene Pulser device (BioRad), expression vector is introduced Chinese hamster ovary celI by electroporation method.Utilize Restriction Enzyme PvuI digestion, obtain linearisation DNA (100 μ g), in cuvette with the Chinese hamster ovary celI (1 * 10 that is suspended in 0.8ml PBS ⁷Cell/ml) mix left standstill 10 minutes on ice, utilized 1.5KV, 25 μ FD capacitor pulses to handle.Room temperature was recovered after 10 minutes, electroporation of cells was added to (GIBCO BRL) cultivation among the CHO-S-SFM II that contains 10% hyclone.Cultivate after 2 days, (GIBCO BRL) continues to cultivate in the CHO-S-SFM II that contains 5nM methotrexate (SIGMA) and 10% hyclone.From the clone of such acquisition, select high expressed rate clone, as the production cell line of 12B5 strand Fv.After cultivating in containing the serum-free medium CHO-S-SFM II (GIBCO BRL) of 5nM methotrexate (SIGMA), the centrifugal separating cell fragment obtains culture supernatant.

7.5 the strand Fv that derives from 12B5 that purifying CHO cell produces

With the culture supernatant of the Chinese hamster ovary celI system of the expression 12B5 strand Fv that obtains in 7.4, utilize anti-FLAG antibody column and gel filtration column purification.

(1) anti-FLAG antibody column

Culture supernatant is added the equilibrated anti-FLAG M2 affinity gel of PBS (Sigma).Utilize same buffer to wash after the post, by the protein that adsorbs on 0.1M glycine-hydrochloride buffer (pH 3.5) eluting post.Elution fraction adds 1M Tris-HCl buffer (pH8.0) immediately and neutralizes.Analyze elution fraction with SDS-PAGE, the component that affirmation is contained strand Fv utilizes Centricon-10 (MILLIPORE) to concentrate.

(2) gel filtration

(1) concentrated solution that obtains in adds with the equilibrated Superdex200 post of PBS (10 * 300mm, AMERSHAM PHARMACIA) that contains 0.01%Tween20.Product sc12B5 eluting is 2 peaks (A, B) (referring to Figure 48).Utilize the 14%-SDS-polyacrylamide gel to analyze component A and B.According to the Laemmli method, having or do not having in the presence of the Reducing agent sample is carried out electrophoresis, use coomassie brilliant blue staining behind the electrophoresis.As shown in figure 49, no matter whether Reducing agent exists, and component A and B produce the single band with the about 31kD of apparent molecular weight.(3.2 * 300mm AMERSHAMPHARMACIA) analyzes component A and B by gel filtration, and component A produces the eluted product of the about 44kD of apparent molecular weight, and B component produces the product (referring to Figure 50 a and b) of 22kD to utilize Superdex200PC 3.2/3.0.This result shows that component A is the non-covalent bond dimer of sc12B5 strand Fv, and B component is a monomer.

7.6 measure the TPO sample agonist activity of various strand Fv

By measuring proliferation activity, estimate the TPO sample activity of anti-MPL single-chain antibody to the Ba/F3 cell (BaF/mpl) of expressing human TPO receptor (MPL).After utilization contained twice in RPMI1640 culture medium (GIBCO) the washing BaF/Mpl cell of 10% hyclone (GIBCO), cell was by 5 * 10 ⁵The cell density of cell/ml is suspended in the culture medium.Dilute anti-MPL single-chain antibody and people TPO (R﹠amp respectively with culture medium; D Systems).The antibody of 50 μ l cell suspension and 50 μ l dilution or people TPO are added in the 96 hole microwell plates (flat) (Falcon), CO ₂(CO in the couveuse ₂Concentration: 5%) cultivated 24 hours.Add 10 μ lWST-8 reagent and (be used to measure the number of germinal cell SF: Nacalai Tesque) after the incubation, utilize fluorescent absorption photometer SPECTRA Fluor (TECAN) to measure absorbance immediately, measure wavelength 450nm, reference wavelength 620nm.At CO ₂(CO in the couveuse ₂Concentration: 5%) incubation utilized SPECTRA Fluor to measure absorbance once more after 2 hours, measured wavelength 450nm, reference wavelength 620nm.Because WST-8 reagent produces chromogenic reaction according to the living cells number at wavelength 450nm place, by the ED50 of following calculating, based on the variation of absorbance after 2 hours, the proliferation activity of evaluation BaF/Mpl.In the breeder reaction curve, be that vertical coordinate, antibody concentration are that abscissa is drawn with the absorbance, the absorbance of plateau is made as 100% response rate.Based near the drawn numerical value of 50% response rate, utilize fitting a straight line (straight lineapproximation) method to obtain fitting formula, calculate the antibody concentration of 50% response rate, be adopted as ED50.

Utilize the COS-7 cells and supernatant of expressing various 12B5 antibody molecules, mensuration is to the agonist activity of MPL, and the result shows shown in Figure 51, has the 12B5IgG of two valency antigen binding sites, its absorbance improves in the concentration dependent mode, and has TPO sample agonist activity (ED50; 29nM), the very weak (ED50 of agonist activity that has the 12B5Fab of monovalent antigen binding site; 34,724nM).On the contrary, have the strand Fv (sc12B5) of monovalent antigen binding site as Fab, show strong agonist activity level, ED50 is 75nM.Yet the variable region of known strand Fv H chain and L chain is by the non-covalent bond combination, so each variable region separates in solution, can combine with the variable region of other molecule, forms polymer, as dimer.When measuring the molecular weight of sc12B5 of gel filtration purification, alleged occurrence is identified as monomer and dimeric molecule (referring to Figure 48).Separate sc12B5 monomer and sc12B5 dimer (referring to Figure 50), measure its agonist activity MPL.Shown in Figure 51 and 52, the monomeric ED50 of sc12B5 is 4438.7nM, and the result who utilizes the COS-7 cells and supernatant is compared in this confirmation, and its agonist activity descends.On the contrary, have the strand Fv (sc12B5 dimer) of two valency antigen binding sites, show high about 400 times agonist activity (ED50 than unit price sc12B5; 10.1nM).In addition, the agonist activity of two valency strand Fv demonstrations is equal to or higher than the agonist activity of people TPO and 12B5IgG.

Embodiment 8

The gene of the variable region of people's antibody 12E10 of the anti-people MPL of structure coding

The encode variable region gene of human monoclonal antibodies 12E10 of anti-people MPL of following structure:

8.1 make up the gene in coding 12E10H chain V district

The nucleotide sequence of SEQ ID NO:86 is to be the gene of the coding of basic engineering in conjunction with the H chain V district of people's antibody 12E10 of people MPL with the described aminoacid sequence of WO99/10494 (SEQ ID NO:85).Full length amino acid sequence is designed to its 5 ' end and connects the targeting sequencing (SEQ ID NO:87) that derives from human immunoglobulin gene (GeneBank accession number AF062252).The nucleotide sequence of design is divided into four oligonucleotide (12E10VH1,12E10VH2,12E10VH3,12E10VH4) that each has the 15bp overlapping sequence.Respectively, 12E10VH1 (SEQ ID NO:88) and 12E10VH3 (SEQ ID NO:90) are that just direction is synthetic, and 12E10VH2 (SEQ ID NO:89) and 12E10VH4 (SEQ ID NO:91) are synthetic for antisense orientation.After assembling each synthetic oligonucleotide according to complementarity separately, add outside primer (12E10VHS and 12E10VHA) amplification full-length gene.Respectively, 12E10VHS (SEQ ID NO:92) is designed to 5 ' end hybridization by forward primer and targeting sequencing, and have Hind III Restriction Enzyme recognition site and a Kozak sequence, and 12E10VHA (SEQ ID NO:93) is designed to by the nucleotide sequence hybridization of reverse primer with coding H chain V district C end, and has donor splicing site sequence and Bam HI Restriction Enzyme recognition site.

100 μ l PCR solution comprise 10 μ l 10x PCR Gold Buffer II, 1.5mMMgCl ₂, 0.08mM dNTPs (dATP, dGTP, dCTP and dTTP), the 5 archaeal dna polymerase AmpliTaq Gold of unit (all from PERKIN ELMER), every kind of synthetic oligonucleotide of 2.5pmol (12E10VH-1 is to-4), 94 ℃ of initial temperatures heating 9 minutes was 94 ℃ 2 minutes, 55 ℃ 2 minutes and 72 ℃ 2 minutes.After this circulation repeats 2 times, add outside primer 12E10VHS of each 100pmol and 12E10VHA.This mixture carries out by 94 ℃ 30 seconds, 55 ℃ 30 seconds and 72 ℃ of circulations of forming in 1 minute 35 times, and 72 ℃ of reheat 5 minutes.

Utilize 1.5% low melting point temperature agarose gel (Sigma) purified pcr product, utilize Restriction Enzyme BamHI and Hind III digestion, and be cloned into people H chain expression vector HEF-g γ 1.Behind the dna sequencing, will comprise the plasmid called after HEF-12E10H-g γ 1 of correct DNA sequence.

HEF-12E10H-g γ 1 usefulness Restriction Enzyme EcoRI and BamHI digestion, the gene of generation coding 12E10VH is cloned into human Fab H chain expression vector pCOS-Fd then, produces pFd-12E10H.Utilize pcr amplification to comprise to include the DNA (SEQ ID NO:63) of the encoding gene of subarea and people H chain part constant region, this intron district is positioned between the encoding gene of people's heavy chain of antibody V district and constant region, this PCR product inserts animal cell expression carrier pCOS1, makes up human Fab H chain expression vector.With HEF-g γ 1 is template, forward primer G1CH1-S (SEQ ID NO:64) (is designed to 5 ' the terminal sequence hybridization with introne 1, and have Restriction Enzyme recognition site EcoRI and a BamHI), reverse primer G1CH1-A (SEQID NO:65) (is designed to 3 ' end DNA hybridization with people H chain constant region CH1 domain, and have the sequence of a part of hinge region of coding, 2 termination codon and a Restriction Enzyme recognition site BglII), in above-mentioned the same terms amplification people H chain constant region gene down.

The nucleotide sequence of the 12E10H chain variable region of the reconstruction that comprises among plasmid HEF-12E10H-g γ 1 and the pFd-12E10H and aminoacid sequence are shown in SEQ ID NO:94.

8.2 make up the gene in coding 12E10L chain V district

The nucleotide sequence of SEQ ID NO:96 is to be the gene of the coding of basic engineering in conjunction with the L chain V district of people's antibody 12E10 of people MPL with the described aminoacid sequence of WO99/10494 (SEQ ID NO:95).Derive from human immunoglobulin gene (Mol.Immunol.1992 by its 5 ' end connection; Targeting sequencing 29:1515-1518) (SEQ ID NO:97) further designs.Nucleotide sequence by above-mentioned same procedure design is divided into four oligonucleotide (12E10VL1,12E10VL2,12E10VL3,12E10VL4) that each has the 15bp overlapping sequence, synthesizes respectively.Respectively, 12E10VL1 (SEQ ID NO:98) and 12E10VL3 (SEQ ID NO:100) have just sequence, and 12E10VL2 (SEQ ID NO:99) and 12E10VL4 (SEQ ID NO:101) have antisense sequences.After assembling each synthetic oligonucleotide according to complementarity separately, (12E10VLS and 12E10VLA) mixes with outside primer, the amplification full-length gene.12E10VLS (SEQ ID NO:102) is designed to 5 ' end hybridization by forward primer and targeting sequencing, and has EcoR I Restriction Enzyme recognition site and Kozak sequence.12E10VLA (SEQ ID NO:103) is designed to by the nucleotide sequence hybridization of reverse primer with coding L chain V district C end, and has BlnI Restriction Enzyme recognition site.

Carry out PCR as mentioned above, utilize 1.5% low melting point temperature agarose gel (Sigma) purified pcr product, utilize Restriction Enzyme EcoRI and BlnI digestion, and be cloned among the pUC19 that comprises people λ chain constant region gene.Behind the dna sequencing, the plasmid that will comprise correct DNA sequence digests with EcoR I, produces the gene of coding 12E10L chain V district and people λ chain constant region, inserts expression vector pCOS-1 then.Plasmid called after pCOS-12E10L with 12E10L chain gene (SEQ ID NO:104).

8.3 produce the 12E10 strand Fv that rebuilds

The 12E10 antibody strand Fv that rebuilds is designed to 12E10VH-joint-12E10VL order, and the C end has FLAG sequence (SEQ ID NO:105) so that detect and purification.Utilization is by (Gly ₄Ser) ₃15 aminoacid of representative or (Gly ₄Ser) ₁The joint sequence that 5 aminoacid of representative are formed makes up the 12E10 strand Fv (sc12E10 and db12E10) of this reconstruction.

(1) utilize the joint sequence of forming by 5 aminoacid to produce the 12E10 strand Fv that rebuilds

With (Gly ₄Ser) ₁The nucleotide sequence of joint is introduced 3 ' end of coding 12E10H chain V district gene and 5 ' end of coding 12E10L chain V district gene, each gene that utilizes PCR to increase respectively and obtain like this, and connect the gene that is increased, make up the encoding gene of the 12E10 strand Fv of the reconstruction that comprises the joint sequence of forming by 5 aminoacid.The 12E10 strand Fv that utilizes four kinds of PCR primers (A-D) preparation to rebuild.Primer A and C have just sequence, and primer B and D have antisense sequences.

The forward primer in H chain V district is 12E10S (primer A, SEQ ID No.106).The reverse primer DB2 in H chain V district (primer B, SEQ ID No.107) is designed to the coding DNA hybridization with H chain V district C end, and has joint (Gly ₄Ser) ₁Coding nucleotide sequence and the coding nucleotide sequence of L chain V district N end.

The forward primer DB1 in L chain V district (primer C, SEQ ID No.108) is designed to the coding DNA hybridization with L chain V district N end, and has joint (Gly ₄Ser) ₁Coding nucleotide sequence and the coding nucleotide sequence of H chain V district C end.The reverse primer 12E10FA in L chain V district (primer D, SEQ ID No.109) is designed to and can hybridizes with the coding DNA of L chain V district C end, and has FLAG coded sequence and NotI Restriction Enzyme recognition site.

In first PCR step, carry out two reactions of A-B, C-D, two kinds of PCR products that first step PCR is obtained are assembled according to complementarity separately.Add after primer A and the D, amplification has the total length coding DNA (the second step PCR) of 12E10 strand Fv joint, that rebuild that is made up of 5 aminoacid.Use the 12E10 of coding and rebuilding among the first step PCR respectively

The plasmid pCOS-12E10L (referring to embodiment 8.2) in the plasmid HEF-12E10H-g γ 1 (referring to embodiment 8.1) in H chain V district and the 12E10L chain V district of coding and rebuilding makes template.

The solution of 50 μ l first step PCR reaction comprises 5 μ l, 10 * PCR Gold Buffer II, 1.5mM MgCl ₂, 0.08mM dNTPs, the 5 archaeal dna polymerase AmpliTaq Gold of unit (from PERKIN ELMER), every kind of primer of 100pmol and every kind of template DNA of 100ng.PCR solution is 94 ℃ of initial temperatures heating 9 minutes, 94 ℃ 30 seconds, 55 ℃ 30 seconds, 72 ℃ 1 minute.This temperature cycles repeats after 35 times, 72 ℃ of reheat reactant mixtures 5 minutes.

Utilize second step PCR assembling product A-B (429bp) and the C-D (395bp).It is template, every kind of each 100pmol of primer, 10 μ l, 10 * PCR Gold Buffer II, 1.5mM MgCl that the second step PCR mixture solution (98 μ l) comprises first step PCR product A-B and every kind 1 μ l of C-D ₂, 0.08mM dNTPs, the 5 archaeal dna polymerase AmpliTaq Gold of unit (from PERKIN ELMER), under above-mentioned the same terms, react.

The 795bpDNA fragment of utilizing 1.5% low melting point temperature agarose gel purification, second step PCR to produce with EcoRI and NotI digestion, is cloned into pCHO1 carrier or pCOS1 carrier.Expression vector pCHO1 utilizes EcoRl and SmaI digestion DHFR-Δ E-RVH-PM1-f (referring to WO92/19759), remove antibody gene, and connects EcoRI-NotI-BamHI joint (TAKARA SHUZO) and make up.Behind the dna sequencing, will comprise the segmental plasmid of coding DNA of the correct aminoacid sequence of 12E10 strand Fv of reconstruction, called after pCHO-db12E10 and pCOS-db12E10.The nucleotide sequence of the 12E10 strand Fv of the reconstruction that comprises among plasmid pCHO-db12E10 and the pCOS-db12E10 and aminoacid sequence are shown in SEQ ID NO:110.

(2) utilize the joint sequence of forming by 15 aminoacid to produce the 12E10 strand Fv that rebuilds

With joint (Gly ₄Ser) ₃Nucleotide sequence introduce 5 ' of 3 ' end of coding 12E10H chain V district gene and coding 12E10L chain V district gene and hold, each gene that utilizes PCR to increase respectively and obtain like this, and connect the gene that is increased, make up the encoding gene of the 12E10 antibody strand Fv of the reconstruction that comprises the joint sequence of forming by 15 aminoacid.The 12E10 strand Fv that utilizes four kinds of PCR primers (A-D) preparation to rebuild.Primer A and C have just sequence, and primer B and D have antisense sequences.

The forward primer in H chain V district is 12E10S (primer A, SEQ ID No.106).The reverse primer sc4.3 in H chain V district (primer B, SEQ ID No.111) is designed to the coding DNA hybridization with H chain V district C end, and has joint (Gly ₄Ser) ₃Coding nucleotide sequence and the coding nucleotide sequence of L chain V district N end.

The forward primer sc1.3 in L chain V district (primer C, SEQ ID No.112) is designed to the coding DNA hybridization with L chain V district N end, and has joint (Gly ₄Ser) ₃Coding nucleotide sequence and the coding nucleotide sequence of H chain V district C end.The reverse primer 12E10FA in L chain V district (primer D, SEQ ID No.109) is designed to and can hybridizes with the coding DNA of L chain V district C end, and has FLAG coded sequence and NotI Restriction Enzyme recognition site.

In first PCR step, carry out two reactions of A-B, C-D, two kinds of PCR products that first step PCR is obtained are assembled according to complementarity separately.Add after primer A and the D, amplification has the total length coding DNA (the second step PCR) of 12E10 strand Fv joint, that rebuild that is made up of 15 aminoacid.Among the first step PCR, make template with the plasmid pCOS-db12E10 (referring to embodiment 8.3 (1)) of the 12E10 strand Fv of coding and rebuilding.

50 μ l solution of first step PCR reaction comprise 5 μ l, 10 * ExTaq Buffer, 0.4mM dNTPs, the 2.5 archaeal dna polymerase TaKaRa ExTaq of unit (from TAKARA), every kind of each 100pmol of primer and every kind of template DNA of 10ng.PCR solution is 94 ℃ of initial temperatures heating 30 seconds, 94 ℃ 15 seconds, 72 ℃ 2 minutes, this circulation repeats 5 times.Repeat after 28 times 72 ℃ of reheat reactant mixtures 5 minutes 94 ℃ of 15 seconds, 72 ℃ circulations of 2 minutes.

Utilize second step assembling PCR product A-B (477bp) and the C-D (447bp).It is template, every kind of primer A and each 100pmol of D, 5 μ l10 * ExTaq Buffer, 0.4mM dNTPs, the 2.5 archaeal dna polymerase TaKaRa ExTaq of unit (from TAKARA) that the second step PCR mixture solution (98 μ l) comprises first step PCR product A-B and every kind 1 μ l of C-D, reacts under above-mentioned the same terms.

The 825bpDNA fragment of utilizing 1.0% low melting point temperature agarose gel purification, second step PCR to produce is with EcoRI and NotI digestion.The dna fragmentation of Huo Deing is cloned into pCHO1 carrier or pCOS1 carrier like this.Behind the dna sequencing, will comprise the coding DNA segmental plasmid called after pCHO-sc12E10 and the pCOS-sc12E10 of the correct aminoacid sequence of 12E10 strand Fv of reconstruction.The nucleotide sequence of the 12E10 strand Fv of the reconstruction that comprises among plasmid pCHO-sc12E10 and the pCOS-sc12E10 and aminoacid sequence are shown in SEQ ID NO:113.

8.4 utilize animal cell expression antibody 12E10 (IgG, Fab) and strand Fv polypeptide

Utilize COS-7 cell or expressing cho cell antibody 12E10 (IgG, Fab) and derive from the strand Fv (5 aminoacid of joint sequence, 15 aminoacid) of antibody 12E10.

Utilize the following transient expression that carries out of COS7 cell.Use Gene Pulser II device (BioRad), carry out transfection by electroporation method.In being suspended in the COS-7 cell of 0.8ml PBS (1 * 10 ⁷Cell/ml), for expressing antibodies 12E10 (IgG) adds above-mentioned expression vector HEF-12E10H-g γ 1 and each 10 μ g of pCOS-12E10L, add pFd-12E10H and each 10 μ g of pCOS-12E10L for expressing the 12E10Fab fragment, add 10 μ g pCOS-sc12E10 (10 μ g) or pCOS-db12E10 (10 μ g) for expressing strand Fv.Utilize 1.5KV, 25 μ FD capacitor pulses to handle the mixture in the cuvette.Room temperature was recovered after 10 minutes, electroporation of cells was added to (GIBCOBRL) cultivation in the DMEM culture medium that contains 10% hyclone.After the overnight incubation, with the PBS washed cell once, be added among the serum-free medium CHO-S-SFM II (GIBCO BRL) and cultivated 3 days.The centrifugal removal cell debris of culture supernatant is with 0.22 μ m membrane filtration.

For setting up the Chinese hamster ovary celI system that stably express derives from the strand Fv (polypeptide) of antibody 12E10, expression vector pCHO-sc12E10 or pCOS-ds12E10 are introduced Chinese hamster ovary celI respectively.

Use Gene Pulser II device (BioRad), every kind of expression vector is introduced Chinese hamster ovary celI by electroporation method.Utilize Restriction Enzyme PvuI digestion, obtain linearisation DNA (100 μ g), in cuvette with the Chinese hamster ovary celI (1 * 10 that is suspended in 0.8ml PBS ⁷Cell/ml) mix left standstill 10 minutes on ice, utilized 1.5KV, 25 μ FD capacitor pulses to handle.Room temperature was recovered after 10 minutes, electroporation of cells was added to (GIBCO BRL) cultivation among the CHO-S-SFM II that contains 10% hyclone of dialysing and nucleic acid.Cultivate after 2 days, (GIBCO BRL) continues to cultivate in containing the free nucleic acid CHO-S-SFM II culture medium of 10% hyclone of dialysing.From the clone of such acquisition, select high expressed rate clone, as the production cell line of 12E10 strand Fv.After cultivating in serum-free CHO-S-SFM II culture medium (GIBCO BRL), the centrifugal removal cell debris of culture supernatant is with 0.22 μ m membrane filtration.

8.5 the strand Fv that derives from 12E10 that purifying CHO cell produces

With the Chinese hamster ovary celI of the expression 12E10 strand Fv (sc12E10, db12E10) of acquisition among the embodiment 8.4 is the culture supernatant that produces, and utilizes anti-FLAG antibody column and gel filtration column purification respectively, produces the strand Fv of purification.

(1) utilizes anti-FLAG antibody column purification

Every kind of culture supernatant (sc12E10, db12E10) is added with 50mM Tris-HCl buffer (pH 7.4) the equilibrated anti-FLAG M2 affinity gel posts (Sigma) that contain 150mM NaCl.Utilize same buffer to wash after the post, by the protein that adsorbs on 100mM glycine buffer (pH3.5) the eluting post.Elution fraction adds 1M Tris-HCl buffer (pH 8.0) immediately and neutralizes, and analyzes with SDS-PAGE.With confirming to contain the component mixing of strand Fv, utilize Centricon-10 (AMICON) to concentrate about 20 times.

(2) gel filtration

(1) concentrated solution that obtains in adds with the equilibrated Superdex200 post of the PBS HR (10 * 300mm, AMERSHAM PHARMACIA) that contains 0.01%Tween20.Tomographic map is shown in Figure 53 and 54.Product sc12E10 eluting is 2 peaks (A, B) (referring to Figure 53).Product db12E10 eluting is 2 peaks (C, D) (referring to Figure 54).Collect each peak component,, have diarrhea electrophoresis sample having or do not have Reducing agent, use coomassie brilliant blue staining behind the electrophoresis according to the Laemmli method.Shown in Figure 55, no matter whether Reducing agent exists, and all components A, B, C and D produce the single band with the about 31kD of apparent molecular weight.When utilizing Superdex200HR to analyze these components by gel filtration, component A produces the product of the about 42kD of apparent molecular weight place eluting, B component produces the product (referring to Figure 56) of 20kD, and component C produces the product of 69kD, and component D produces the product (referring to Figure 57) of 41kD.This results suggest, the component A in sc12E10 source is the non-covalent bond dimer of strand Fv, and B component is the monomer of strand Fv, and the component C in db12E10 source is the non-covalent bond trimer of strand Fv, and component D is the non-covalent bond dimer of strand Fv.

8.6 measure the TPO sample agonist activity of various strand Fv

By measuring proliferation activity, estimate the TPO sample activity of anti-mpl single-chain antibody to the Ba/F3 cell (BaF/mpl) of expressing human TPO receptor (MPL).

After utilization contained twice in RPMI1640 culture medium (GIBCO) the washing BaF/mpl cell of 1% hyclone (GIBCO), cell was by 5 * 10 ⁵The cell density of cell/ml is suspended in the culture medium.Dilute anti-MPL single-chain antibody or people TPO (R﹠amp respectively with culture medium; DSystems).The antibody of 50 μ l cell suspension and 50 μ l dilution or people TPO are added in the 96 hole microwell plates (flat) (Corning), at CO ₂(CO in the couveuse ₂Concentration: 5%) cultivated 24 hours.Incubation adds 10 μ l WST-8 reagent later on and (is used to measure the reagent of germinal cell SF number: Nacalai Tesque), utilize absorption spectrophotometry Benchmark Plus (BioRad) to measure absorbance immediately, measure wavelength 450nm, reference wavelength 655nm.At CO ₂(CO in the couveuse ₂Concentration: 5%) incubation utilized Benchmark Plus to measure absorbance once more after 2 hours, measured wavelength 450nm, reference wavelength 655Bm.Because WST-8 reagent produces chromogenic reaction according to the living cells number at wavelength 450nm place, based on the variation of absorbance after 2 hours, the proliferation activity of evaluation BaF/mpl.

Utilize the COS-7 cells and supernatant of expressing various 12E10 antibody molecules, measure agonist activity, shown in Figure 58 MPL.Have the strand Fv of 5 aminoacid joints (ds12E10) and 15 aminoacid joints (sc12E10), its absorbance improves in the concentration dependent mode, shows TPO sample agonist activity (ED50; Difference 9pM and 51pM), and 12E10IgG and 12E10Fab do not have activity.

Known strand Fv H chain and the not only intramolecularly combination of L chain, and intermolecular in conjunction with forming polymer, for example dimer.The culture supernatant of the Chinese hamster ovary celI of expression 12E10 strand Fv is measured the agonist activity to MPL through gel filtration.The result is shown in Figure 59.The a small amount of dimer that comprises among the sc12E10 shows than monomer (sc12E10 monomer, ED50; 10nM) powerful about 5000 times TPO sample agonist activity (sc12E10 dimer, ED50; 1.9pM).Activity is higher than TPO (ED50; 27pM).The dimer of db12E10 (db12E10 dimer, ED50; 2.0pM) the demonstration high activity suitable with the sc12E10 dimer.The trimer of db12E10 (molecular weight that obtains from gel filtration is speculated as trimer) (ED50; 7.4pM) show and to be lower than the dimeric high activity of db12E10.These results suggest, two valency antigen binding sites (dimer) are important for the activity of agonist antibody 12E10.In view of 12E10IgG does not have activity, infer that the other factors beyond two valencys is important, for example the location of antigen binding site, distance or angle.

Description of drawings

Fig. 1 represents the flow cytometry result, shows the not L1210 cell (hIAP/L1210) of associative list intelligent IAP of human IgG antibody.

Fig. 2 represents the flow cytometry result, shows the L1210 cell (hIAP/L1210) of chimeric MABL-1 antibody specificity associative list intelligent IAP.

Fig. 3 represents the flow cytometry result, shows the L1210 cell (hIAP/L1210) of chimeric MABL-2 antibody specificity associative list intelligent IAP.

The production process sketch map of Fig. 4 strand Fv of the present invention.

Fig. 5 illustrates the structure of expression plasmid, and this plasmid is used in E.coliMiddle coding DNA of expressing strand Fv of the present invention.

Fig. 6 illustrates the structure of expression plasmid, and this plasmid is used in the coding DNA of expressing strand Fv of the present invention in the mammalian cell.

Fig. 7 shows the figure as a result of Western trace among the embodiment 5.4.Begin to, molecular weight sign (from top demonstration 97.4,66,45,31,21.5 and 14.5kDa) from the left side, introduce the COS7 cells and supernatant of pCHO1 and the COS7 cells and supernatant of introducing pCHOM2.Show the reconstruction strand Fv (arrow) that in the COS7 cells and supernatant of introducing pCHOM2, comprises antibody MABL-2.

Fig. 8 represents the flow cytometry result, shows the antibody in the pCHO1/COS7 cells and supernatant that compares, not in conjunction with the pCOS1/L1210 control cells.

Fig. 9 represents the flow cytometry result, shows the antibody in the MABL2-scFv/COS7 cells and supernatant, not in conjunction with the pCOS1/L1210 control cells.

Figure 10 represents the flow cytometry result, shows that antibody in the pCOS1/COS7 cells and supernatant that compares is not in conjunction with the hIAP/L1210 cell.

Figure 11 represents the flow cytometry result, shows that antibody specificity in the MABL2-scFv/COS7 cells and supernatant is in conjunction with the hIAP/L1210 cell.

Figure 12 represents competitive ELISA result among the embodiment 5.6, be indication wherein with inhibitory action to mouse monoclonal antibody MABL-2 conjugated antigen, compare with the pCHO1/COS7 cells and supernatant of contrast, illustrate the antigen-binding activity of strand Fv of the present invention (MABL2-scFv).

Figure 13 represents the result of apoptosis induction effect among the embodiment 5.7, shows the antibody in the pCHO1/COS7 cells and supernatant that compares, and can not induce pCOS1/L1210 control cells apoptosis.

Figure 14 represents the result of apoptosis induction effect among the embodiment 5.7, shows the antibody in the MABL2-scFv/COS7 cells and supernatant, can not induce pCOS1/L1210 control cells apoptosis.

Figure 15 represents the result of apoptosis induction effect among the embodiment 5.7, shows the antibody in the pCHO1/COS7 cells and supernatant that compares, and can not induce the hIAP/L1210 apoptosis.

Figure 16 represents the result of apoptosis induction effect among the embodiment 5.7, shows the antibody in the MABL2-scFv/COS7 cells and supernatant, and specificity is induced the hIAP/L1210 apoptosis.

Figure 17 represents the result of apoptosis induction effect among the embodiment 5.7, shows the antibody in the pCHO1/COS7 cells and supernatant that compares, and can not induce CCRF-CEM apoptosis (final concentration 50%).

Figure 18 represents the result of apoptosis induction effect among the embodiment 5.7, shows the antibody in the MABL2-scFv/COS7 cells and supernatant, and specificity is induced CCRF-CEM apoptosis (final concentration 50%).

Figure 19 represents the resulting tomographic results of strand Fv that comes from antibody MABL-2 that embodiment 5.9 purifying CHO cells produce, when showing with the hydroxyapatite column purification from the component of Blue-sepharose post, and the main peaks of acquisition, component A and B component.

Figure 20 represents the component A that embodiment 5.9-(2) obtains and the gel filtration purification result of B component, show component A at apparent molecular weight about 36kD place and B component in the main peaks (being respectively AI and BI) of about 76kD place eluting.

Figure 21 shows the single band of observing the about 35kD of molecular weight in two components for the SDS-PAGE of the strand component that Fv obtains that comes from antibody MABL-2 that embodiment 5.9 purifying CHO cells produce analyzes.

Figure 22 represents that the strand Fv that comes from antibody MABL-2 of gel filtration purifying CHO cell generation obtains the analysis result of component AI and BI, and wherein component AI comprises monomer, and B component I comprises dimer.

Figure 23 illustrates the structure of expression plasmid, and it is used in E.coliMiddle coding DNA of expressing strand Fv of the present invention.

Figure 24 represents embodiment 5.12 gel filtration column purifications E.coliThe result of the strand Fv polypeptide crude product that comes from antibody MABL-2 that produces, wherein each peak is represented respectively E.coliMonomer or the dimer of the strand Fv that produces.

Figure 25 represents the result of apoptosis induction effect among the embodiment 5.13, shows the mouse IgG antibody that compares, and can not induce hIAP/L1210 apoptosis (final concentration 3 μ g/ml).

Figure 26 represents the result of apoptosis induction effect among the embodiment 5.13, shows the MABL2-scFv dimer that Chinese hamster ovary celI produces, and significantly induces hIAP/L1210 apoptosis (final concentration 3 μ g/ml).

Figure 27 represents the result of apoptosis induction effect among the embodiment 5.13, shows E.coliThe MABL2-scFv dimer that produces is significantly induced hIAP/L1210 apoptosis (final concentration 3 μ g/ml).

Figure 28 represents the result of apoptosis induction effect among the embodiment 5.13, shows the apoptosis induction of the MABL2-scFv monomer of Chinese hamster ovary celI generation to the hIAP/L1210 cell, is in par (final concentration 3 μ g/ml) with contrast.

Figure 29 represents the result of apoptosis induction effect among the embodiment 5.13, shows E.coliThe MABL2-scFv monomer that produces is in par (final concentration 3 μ g/ml) to the apoptosis induction of hIAP/L1210 cell with contrast.

Figure 30 represents the result of apoptosis induction effect among the embodiment 5.13, shows the mouse IgG antibody that compares, even add anti-FLAG antibody, can not induce hIAP/L1210 apoptosis (final concentration 3 μ g/ml).

Figure 31 represents the result of apoptosis induction effect among the embodiment 5.13, shows the MABL2-scFv monomer that Chinese hamster ovary celI produces, and when adding anti-FLAG antibody, significantly induces hIAP/L1210 apoptosis (final concentration 3 μ g/ml).

Figure 32 is illustrated in the serum of human myeloma cell line KPMM2 transplanting mice, the quantitative measurement result of human IgG, the amount of the human IgG that is produced by human myeloma cell in the demonstration mice.Show that the scFv/CHO dimer significantly suppresses the growth of KPMM2 cell.

Figure 33 represents the time-to-live of mice after transplantation tumor, shows scFv/CHO dimer administration group time-to-live significant prolongation.

Figure 34 illustrates the structure of expression plasmid, and it can express the antibody [sc (Fv) of the change that comprises two H chain V districts coming from antibody MABL-2 and two L chain V districts ₂].

Figure 35 illustrates the structure of expressing scFv (HL type) plasmid, and wherein the V district connects by [H chain]-[L chain] mode that does not have peptide linker.

Figure 36 illustrates the structure of HL type polypeptide and the aminoacid sequence of peptide linker.

Figure 37 illustrates the structure of expressing scFv (LH type) plasmid, and wherein the V district connects by [L chain]-[H chain] mode that does not have peptide linker.

Figure 38 illustrates the structure of LH type polypeptide and the aminoacid sequence of peptide linker.

Figure 39 represents the result of embodiment 6.4Western trace, shows the antibody sc (Fv) that has expressed the change that comprises two H chain V districts and two L chain V districts ₂, and MABL2-scFv with different length peptide linker.

Figure 40 a and 40b represent the flow cytometry result of the COS7 cells and supernatant of embodiment 6.3 (1) preparations, show MABL2-scFv and the sc (Fv) with different length peptide linker ₂People IAP had high-affinity.

Figure 41 a and 41b represent the result of embodiment 6.6 apoptosis induction effects, show scFv＜HL3,4,6,7, LH3,4,6 and 7〉and sc (Fv) ₂, significantly induce the hIAP/L1210 cell death.

Figure 42 represents the evaluation result of embodiment 6.10 antigen binding capacities, shows scFv＜HL5〉dimer and sc (Fv) ₂People IAP had high-affinity.

Figure 43 represents embodiment 6.11 ExternalThe result of apoptosis induction effect shows scFv＜HLS〉dimer and sc (Fv) ₂Induce hIAP/L1210 cell and CCRF-CEM apoptosis in concentration dependence mode.

Figure 44 is illustrated in the mice serum of transplanting human myeloma cell, by the proteinic quantitative measurement result of M of human myeloma cell line KPMM2 generation.Show scFv＜HL5〉dimer and sc (Fv) ₂Significantly suppress the growth of KPMM2 cell.

Figure 45 represent mice after the tumour transplatation time-to-live (my god), show scFv＜HL5 the time-to-live significant prolongation of administration group.

Figure 46 represent mice after the tumour transplatation time-to-live (my god), show sc (Fv) ₂The time-to-live significant prolongation of administration group.

Figure 47 illustrates segmental method of coding DNA and the structure thereof that makes up the reconstruction 12B5 strand Fv that comprises the joint sequence of being made up of 15 aminoacid.

Figure 48 represents the result who passes through every kind of 12B5 strand of gel filtration purification Fv of acquisition among the embodiment 7.5 (1), shows that sc12B5 is divided into two peaks (component A and B).

Figure 49 represents each the component A that carries out among the embodiment 7.5 (2) and the SDS-PAGE analysis result of B.

Figure 50 represents each the component A that carries out among the embodiment 7.5 (2) and the Superdex200 post analysis result of B, the main peak that shows component A is at the eluting of about 44kD apparent molecular weight place shown in (a), and the main peak of B component is at the eluting of about 22kD apparent molecular weight place shown in (b).

Figure 51 represents the measurement result of the TPO sample agonist activity of sc12B5 and antibody 12B5 (IgG, Fab), shows that 12B5IgG and unit price strand Fv (sc12B5) show TPO sample agonist activity in the concentration dependent mode.

Figure 52 represents the measurement result of sc12B5 monomer and dimeric TPO sample agonist activity, the agonist activity that shows the strand Fv (sc12B5 dimer) with two valency antigen binding sites is higher than about 400 times of unit price sc12B5, and effectiveness is equal to or higher than people TPO.

Figure 53 represents to utilize the purification result of Superdex200HR post by the sc12E10 single-chain antibody of gel permeation chromatography acquisition, shows that sc12E10 is divided into two peaks (component A and B).

Figure 54 represents to utilize the purification result of Superdex200HR post by the db12E10 single-chain antibody of gel permeation chromatography acquisition, shows that db12E10 is divided into two peaks (component C and D).

Figure 55 represents component A and B (sc12E10) and component C and the SDS-PAGE analysis of D (db12E10) under reduction or non-reduced condition.

Figure 56 represents to utilize the Superdex200HR post by the component A of gel permeation chromatography and the analysis result of B, and the main peak that shows (1) component A is at about 42kD apparent molecular weight place eluting, and the main peak of (2) B component is at about 20kD apparent molecular weight place eluting.。

Figure 57 represents to utilize the Superdex200HR post by the component C of gel permeation chromatography and the analysis result of D, and the main peak that shows (1) component C is at about 69kD apparent molecular weight place eluting, and the main peak of (2) component D is at about 41kD apparent molecular weight place eluting.。

Figure 58 illustrates the agonist activity of various 12E10 antibody molecules to MPL, show that strand Fv (sc12E10, db12E10) shows TPO sample agonist activity, and 12E10IgG and 12E10Fab does not show.

The agonist activity of MPL when Figure 59 illustrates sc12E10 monomer and dimer and db12E10 dimer and trimer shows that sc12E10 dimer and db12E10 dimer and trimer show the TPO sample agonist activity that is higher than TPO.

Commercial Application

The antibody of change of the present invention has can transduction signal enters the activator effect of cell by crosslinked cell surface molecule, and advantage is that the molecular size of comparing parental antibody molecule (fully IgG) because of it descends to the permeability height of tissue and tumour. The invention provides and compare the antibody that natural aglucon (for example TPO) and parental antibody (fully IgG) have the change of significantly high agonist activity. Even parental antibody does not have agonist activity, also can provide and compare the antibody that natural aglucon has the change of higher agonist activity. This compares with original antibody owing to the antibody that changes, and shape is closer to aglucon. Therefore, the antibody of this change can be used for the signal transduction activator,, inducing cell propagation apoptosis-induced to realize, Cell differentiation inducing activity, inducing cell division or Cycle Regulation effect. According to the present invention, antibody molecule is changed into the antibody of change, cause reducing the crosslinked side effect that causes of iuntercellular, and the newtype drug of only inducing required effect by crosslinked cell surface molecule is provided. The pharmaceutical preparation that comprises antibody that the present invention changes and be active component can be used as and prevents and/or treats medicine, be used for cancer, inflammation, hormone disturbance, autoimmune disease and blood disease, for example leukaemia, malignant lymphoma, alpastic anemia, Myelodysplastic syndromes and polycythemia vera.

Sequence table

<110>CHUGAI SEIYAKU KABUSHIKI KAISHA

＜120〉degraded agonist antibody

<130>FP1032

<141>2001-10-22

<150>JP2000-321821

<151>2000-10-20

<150>JP2000-321822

<151>2000-10-20

<150>PCT/JP01/01912

<151>2001-03-12

<150>PCT/JP01/03288

<151>2001-04-17

<150>JP2001-277314

<151>2001-09-12

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＜223〉PCR primer

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ggatcccggg tggatggtgg gaagatg 27

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＜213〉artificial sequence

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＜223〉PCR primer

<400>3

ggatcccggg ccagtggata gacagatg 28

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<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>4

ggatcccggg agtggataga ccgatg 26

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<212>DNA

<213>Mus

<220>

<221>CDS

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<400>5

atg aag ttg cct gtt agg ctg ttg gtg ctg atg ttc tgg att cct gcg 48

Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala

1 5 10 15

tcc agc agt gat gtt gtg atg acc caa act cca ctc tcc ctg cct gtc 96

Ser Ser Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val

20 25 30

agt ctt gga gat caa gcc tcc atc tct tgc aga tct agt cag agc ctt 144

Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu

35 40 45

cta cac agt aaa gga aac acc tat tta caa tgg tac cta cag aag cca 192

Leu His Ser Lys Gly Asn Thr Tyr Leu Gln Trp Tyr Leu Gln Lys Pro

50 55 60

ggc cag tct cca aag ctc ctg atc tac aaa gtt tcc aac cga ttt tct 240

Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser

65 70 75 80

ggg gtc cca gac agg ttc agt ggc agt gga tca ggg aca gat ttc aca 288

Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr

85 90 95

ctc aag atc agc aga gtg gag gct gag gat ctg gga gtt tat ttc tgc 336

Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys

100 105 110

tct caa agt aca cat gtt ccg tac acg tcc gga ggg ggg acc aag ctg 384

Ser Gln Ser Thr His Val Pro Tyr Thr Ser Gly Gly Gly Thr Lys Leu

115 120 125

gaa ata aaa c 394

Glu Ile Lys

130

<210>6

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<212>DNA

<213>Mus

<220>

<221>CDS

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＜223〉pGEM-M1H.1-57; Signal peptide, 58-409; Mature peptide

<400>6

atg gaa tgg agc tgg ata ttt ctc ttc ctc ctg tca gga act gca ggt 48

Met Glu Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly

1 5 10 15

gtc cac tcc cag gtc cag ctg cag cag tct gga cct gac ctg gta aag 96

Val His Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Asp Leu Val Lys

20 25 30

cct ggg gct tca gtg aag atg tcc tgc aag gct tct gga tac acc ttc 144

Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe

35 40 45

gtt aac cat gtt atg cac tgg gtg aag cag aag cca ggg cag ggc ctt 192

Val Asn His Val Met His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu

50 55 60

gag tgg att gga tat att tat cct tac aat gat ggt act aag tac aat 240

Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr Lys Tyr Asn

65 70 75 80

gag aag ttc aag ggc aag gcc aca ctg act tca gag aaa tcc tcc agc 288

Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ser Glu Lys Ser Ser Ser

85 90 95

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Ala Ala Tyr Met Glu Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val

100 105 110

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Tyr Tyr Cys Ala Arg Gly Gly Tyr Tyr Ser Tyr Asp Asp Trp Gly Gln

115 120 125

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Gly Thr Thr Leu Thr Val Ser Ser

130 135

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Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phc Trp Ile Pro Gly

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Ser Ser Ser Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val

20 25 30

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Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu

35 40 45

gtg cac agt aat gga aag acc tat tta cat tgg tac ctg cag aag cca 192

Val His Ser Asn Gly Lys Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro

50 55 60

ggc cag tct cca aaa ctc ctg atc tac aaa gtt tcc aac cga ttt tct 240

Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser

65 70 75 80

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Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Val Thr Asp Phe Thr

85 90 95

ctc atg atc agc aga gtg gag gct gag gat ctg gga gtt tat ttc tgc 336

Leu Met Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys

100 105 110

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Ser Gln Ser Thr His Val Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu

115 120 125

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Glu Ile Lys

130

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Met Glu Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly

1 5 10 15

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Val His Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys

20 25 30

cct ggg gct tca gtg aag atg tcc tgc aag gct tct gga tac acc ttc 144

Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe

35 40 45

gct aac cat gtt att cac tgg gtg aag cag aag cca ggg cag ggc ctt 192

Ala Asn His Val Ile His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu

50 55 60

gag tgg att gga tat att tat cct tac aat gat ggt act aag tat aat 240

Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr Lys Tyr Asn

65 70 75 80

gag aag ttc aag gac aag gcc act ctg act tca gac aaa tcc tcc acc 288

Glu Lys Phe Lys Asp Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Thr

85 90 95

aca gcc tac atg gac ctc agc agc ctg gcc tct gag gac tct gcg gtc 336

Thr Ala Tyr Met Asp Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val

100 105 110

tat tac tgt gca aga ggg ggt tac tat act tac gac gac tgg ggc caa 384

Tyr Tyr Cys Ala Arg Gly Gly Tyr Tyr Thr Tyr Asp Asp Trp Gly Gln

115 120 125

ggc acc act ctc aca gtc tcc tca g 409

Gly Thr Thr Leu Thr Val Ser Ser

130 135

<210>9

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＜213〉artificial sequence

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＜223〉PR primer

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cccaagcttc caccatgaag ttgcctgtta gg 32

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＜213〉artificial sequence

<220>

＜223〉PCR primer

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cccaagcttc caccatggaa tggagctgga ta 32

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<211>34

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>11

cgcggatcca ctcacgtttt atttccagct tggt 34

<210>12

<211>34

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>12

cgcggatcca ctcacctgag gagactgtga gagt 34

<210>13

<211>30

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>13

catgccatgg cgcaggtcca gctgcagcag 30

<210>14

<211>27

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>14

accaccacct gaggagactg tgagagt 27

<210>15

<211>27

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>15

gtctcctcag gtggtggtgg ttcgggt 27

<210>16

<211>27

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>16

cacaacatcc gatccgccac cacccga 27

<210>17

<211>27

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>17

ggcggatcgg atgttgtgat gacccaa 27

<210>18

<211>57

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>18

ccggaattct cattatttat cgtcatcgtc tttgtagtct tttatttcca gcttggt 57

<210>19

<211>45

<212>DNA

＜213〉artificial sequence

<220>

＜223〉joint aminoacid sequence and nucleotide sequence

<400>19

ggt ggt ggt ggt tcg ggt ggt ggt ggt tcg ggt ggt ggc gga tcg 45

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser

5 10 15

<210>20

<211>828

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(822)

<223>pscM1.MABL1-scFv

<400>20

atg aaa tac cta ttg cct acg gca gcc gct gga ttg tta tta ctc gct 48

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala

1 5 10 15

gcc caa cca gcc atg gcg cag gtc cag ctg cag cag tct gga cct gac 96

Ala Gln Pro Ala Met Ala Gln Val Gln Leu Gln Gln Ser Gly Pro Asp

20 25 30

ctg gta aag cct ggg gct tca gtg aag atg tcc tgc aag gct tct gga 144

Leu Val Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly

35 40 45

tac acc ttc gtt aac cat gtt atg cac tgg gtg aag cag aag cca ggg 192

Tyr Thr Phe Val Asn His Val Met His Trp Val Lys Gln Lys Pro Gly

50 55 60

cag ggc ctt gag tgg att gga tat att tat cct tac aat gat ggt act 240

Gln Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr

65 70 75 80

aag tac aat gag aag ttc aag ggc aag gcc aca ctg act tca gag aaa 288

Lys Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ser Glu Lys

85 90 95

tcc tcc agc gca gcc tac atg gag ctc agc agc ctg gcc tct gag gac 336

Ser Ser Ser Ala Ala Tyr Met Glu Leu Ser Ser Leu Ala Ser Glu Asp

100 105 110

tct gcg gtc tac tac tgt gca aga ggg ggt tac tat agt tac gac gac 384

Ser Ala Val Tyr Tyr Cys Ala Arg Gly Gly Tyr Tyr Ser Tyr Asp Asp

115 120 125

tgg ggc caa ggc acc act ctc aca gtc tcc tca ggt ggt ggt ggt tcg 432

Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser

130 135 140

ggt ggt ggt ggt tcg ggt ggt ggc gga tcg gat gtt gtg atg acc caa 480

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln

145 150 155 160

act cca ctc tcc ctg cct gtc agt ctt gga gat caa gcc tcc atc tct 528

Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser

165 170 175

tgc aga tct agt cag agc ctt cta cac agt aaa gga aac acc tat tta 576

Cys Arg Ser Ser Gln Ser Leu Leu His Ser Lys Gly Asn Thr Tyr Leu

180 185 190

caa tgg tac cta cag aag cca ggc cag tct cca aag ctc ctg atc tac 624

Gln Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr

195 200 205

aaa gtt tcc aac cga ttt tct ggg gtc cca gac agg ttc agt ggc agt 672

Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser

210 215 220

gga tca ggg aca gat ttc aca ctc aag atc agc aga gtg gag gct gag 720

Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu

225 230 235 240

gat ctg gga gtt tat ttc tgc tct caa agt aca cat gtt ccg tac acg 768

Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Tyr Thr

245 250 255

tcc gga ggg ggg acc aag ctg gaa ata aaa gac tac aaa gac gat gac 816

Ser Gly Gly Gly Thr Lys Leu Glu Ile Lys Asp Tyr Lys Asp Asp Asp

260 265 270

gat aaa taatga 828

Asp Lys

<210>21

<211>31

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>21

acgcgtcgac tcccaggtcc agctgcagca g 31

<210>22

<211>18

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>22

gaaggtgtat ccagaagc 18

<210>23

<211>819

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(813)

<223>pCHOM1.MABL1-scFv

<400>23

atg gga tgg agc tgt atc atc ctc ttc ttg gta gca aca gct aca ggt 48

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly

1 5 10 15

gtc gac tcc cag gtc cag ctg cag cag tct gga cct gac ctg gta aag 96

Val Asp Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Asp Leu Val Lys

20 25 30

cct ggg gct tca gtg aag atg tcc tgc aag gct tct gga tac acc ttc 144

Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe

35 40 45

gtt aac cat gtt atg cac tgg gtg aag cag aag cca ggg cag ggc ctt 192

Val Asn His Val Met His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu

50 55 60

gag tgg att gga tat att tat cct tac aat gat ggt act aag tac aat 240

Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr Lys Tyr Asn

65 70 75 80

gag aag ttc aag ggc aag gcc aca ctg act tca gag aaa tcc tcc agc 288

Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ser Glu Lys Ser Ser Ser

85 90 95

gca gcc tac atg gag ctc agc agc ctg gcc tct gag gac tct gcg gtc 336

Ala Ala Tyr Met Glu Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val

100 105 110

tac tac tgt gca aga ggg ggt tac tat agt tac gac gac tgg ggc caa 384

Tyr Tyr Cys Ala Arg Gly Gly Tyr Tyr Ser Tyr Asp Asp Trp Gly Gln

115 120 125

ggc acc act ctc aca gtc tcc tca ggt ggt ggt ggt tcg ggt ggt ggt 432

Gly Thr Thr Lou Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly

130 135 140

ggt tcg ggt ggt ggc gga tcg gat gtt gtg atg acc caa act cca ctc 480

Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu

145 150 155 160

tcc ctg cct gtc agt ctt gga gat caa gcc tcc atc tct tgc aga tct 528

Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser

165 170 175

agt cag agc ctt cta cac agt aaa gga aac acc tat tta caa tgg tac 576

Ser Gln Ser Leu Leu His Ser Lys Gly Asn Thr Tyr Leu Gln Trp Tyr

180 185 190

cta cag aag cca ggc cag tct cca aag ctc ctg atc tac aaa gtt tcc 624

Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser

195 200 205

aac cga ttt tct ggg gtc cca gac agg ttc agt ggc agt gga tca ggg 672

Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly

210 215 220

aca gat ttc aca ctc aag atc agc aga gtg gag gct gag gat ctg gga 720

Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly

225 230 235 240

gtt tat ttc tgc tct caa agt aca cat gtt ccg tac acg tcc gga ggg 768

Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Tyr Thr Ser Gly Gly

245 250 255

ggg acc aag ctg gaa ata aaa gac tac aaa gac gat gac gat aaa taa 816

Gly Thr Lys Leu Glu Ile Lys Asp Tyr Lys Asp Asp Asp Asp Lys

260 265 270

tga 819

<210>24

<211>828

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(822)

<223>pscM2.MABL2-scFv

<400>24

atg aaa tac cta ttg cct acg gca gcc gct gga ttg tta tta ctc gct 48

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala

1 5 10 15

gcc caa cca gcc atg gcg cag gtc cag ctg cag cag tct gga cct gaa 96

Ala Gln Pro Ala Met Ala Gln Val Gln Leu Gln Gln Ser Gly Pro Glu

20 25 30

ctg gta aag cct ggg gct tca gtg aag atg tcc tgc aag gct tct gga 144

Leu Val Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly

35 40 45

tac acc ttc gct aac cat gtt att cac tgg gtg aag cag aag cca ggg 192

Tyr Thr Phe Ala Asn His Val Ile His Trp Val Lys Gln Lys Pro Gly

50 55 60

cag ggc ctt gag tgg att gga tat att tat cct tac aat gat ggt act 240

Gln Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr

65 70 75 80

aag tat aat gag aag ttc aag gac aag gcc act ctg act tca gac aaa 288

Lys Tyr Asn Glu Lys Phe Lys Asp Lys Ala Thr Leu Thr Ser Asp Lys

85 90 95

tcc tcc acc aca gcc tac atg gac ctc agc agc ctg gcc tct gag gac 336

Ser Ser Thr Thr Ala Tyr Met Asp Leu Ser Ser Leu Ala Sor Glu Asp

100 105 110

tct gcg gtc tat tac tgt gca aga ggg ggt tac tat act tac gac gac 384

Ser Ala Val Tyr Tyr Cys Ala Arg GLy Gly Tyr Tyr Thr Tyr Asp Asp

115 120 125

tgg ggc caa ggc acc act ctc aca gtc tcc tca ggt ggt ggt ggt tcg 432

Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser

130 135 140

ggt ggt ggt ggt tcg ggt ggt ggc gga tcg gat gtt gtg atg acc caa 480

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln

145 150 155 160

agt cca ctc tcc ctg cct gtc agt ctt gga gat caa gcc tcc atc tct 528

Ser Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser

165 170 175

tgc aga tca agt cag agc ctt gtg cac agt aat gga aag acc tat tta 576

Cys Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Lys Thr Tyr

180 185 190

cat tgg tac ctg cag aag cca ggc cag tct cca aaa ctc ctg atc tac 624

His Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr

195 200 205

aaa gtt tcc aac cga ttt tct ggg gtc cca gac agg ttc agt ggc agt 672

Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser

210 215 220

gga tca gtg aca gat ttc aca ctc atg atc agc aga gtg gag gct gag 720

Gly Ser Val Thr Asp Phe Thr Leu Met Ile Ser Arg Val Glu Ala Glu

225 230 235 240

gat ctg gga gtt tat ttc tgc tct caa agt aca cat gtt ccg tac acg 768

Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Tyr Thr

245 250 255

ttc gga ggg ggg acc aag ctg gaa ata aaa gac tac aaa gac gat gac 816

Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Asp Tyr Lys Asp Asp Asp

260 265 270

gat aaa taatga 828

Asp Lys

<210>25

<211>819

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(813)

<223>pCHOM2.MABL2-scFv

<400>25

atg gga tgg agc tgt atc atc ctc ttc ttg gta gca aca gct aca ggt 48

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly

1 5 10 15

gtc gac tcc cag gtc cag ctg cag cag tct gga cct gaa ctg gta aag 96

Val Asp Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys

20 25 30

cct ggg gct tca gtg aag atg tcc tgc aag gct tct gga tac acc ttc 144

Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe

35 40 45

gct aac cat gtt att cac tgg gtg aag cag aag cca ggg cag ggc ctt 192

Ala Asn His Val Ile His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu

50 55 60

gag tgg att gga tat att tat cct tac aat gat ggt act aag tat aat 240

Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr Lys Tyr Asn

65 70 75 80

gag aag ttc aag gac aag gcc act ctg act tca gac aaa tcc tcc acc 288

Glu Lys Phe Lys Asp Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Thr

85 90 95

aca gcc tac atg gac ctc agc agc ctg gcc tct gag gac tct gcg gtc 336

Thr Ala Tyr Met Asp Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val

100 105 110

tat tac tgt gca aga ggg ggt tac tat act tac gac gac tgg ggc caa 384

Tyr Tyr Cys Ala Arg Gly Gly Tyr Tyr Thr Tyr Asp Asp Trp Gly Gln

115 120 125

ggc acc act ctc aca gtc tcc tca ggt ggt ggt ggt tcg ggt ggt ggt 432

Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly

130 135 140

ggt tcg ggt ggt ggc gga tcg gat gtt gtg atg acc caa agt cca ctc 480

Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Ser Pro Leu

145 150 155 160

tcc ctg cct gtc agt ctt gga gat caa gcc tcc atc tct tgc aga tca 528

Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser

165 170 175

agt cag agc ctt gtg cac agt aat gga aag acc tat tta cat tgg tac 576

Ser Gln Ser Leu Val His Ser Asn Gly Lys Thr Tyr Leu His Trp Tyr

180 185 190

ctg cag aag cca ggc cag tct cca aaa ctc ctg atc tac aaa gtt tcc 624

Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser

195 200 205

aac cga ttt tct ggg gtc cca gac agg ttc agt ggc agt gga tca gtg 672

Asn Arg Phc Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Val

210 215 220

aca gat ttc aca ctc atg atc agc aga gtg gag gct gag gat ctg gga 720

Thr Asp Phe Thr Leu Met Ile Ser Arg Val Glu Ala Glu Asp Leu Gly

225 230 235 240

gtt tat ttc tgc tct caa agt aca cat gtt ccg tac acg ttc gga ggg 768

Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Tyr Thr Phe Gly Gly

245 250 255

ggg acc aag ctg gaa ata aaa gac tac aaa gac gat gac gat aaa taa 816

Gly Thr Lys Leu Glu Ile Lys Asp Tyr Lys Asp Asp Asp Asp Lys

260 265 270

tga 819

<210>26

<211>456

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(450)

＜223〉pCHO-shIAP. soluble human IAP

<400>26

atg tgg ccc ctg gta gcg gcg ctg ttg ctg ggc tcg gcg tgc tgc gga 48

Met Trp Pro Leu Val Ala Ala Leu Leu Leu Gly Ser Ala Cys Cys Gly

1 5 10 15

tca gct cag cta cta ttt aat aaa aca aaa tct gta gaa ttc acg ttt 96

Ser Ala Gln Leu Leu Phe Ash Lys Thr Lys Ser Val Glu Phe Thr Phe

20 25 30

tgt aat gac act gtc gtc att cca tgc ttt gtt act aat atg gag gca 144

Cys Asn Asp Thr Val Val Ile Pro Cys Phe Val Thr Asn Met Glu Ala

35 40 45

caa aac act act gaa gta tac gta aag tgg aaa ttt aaa gga aga gat 192

Gln Asn Thr Thr Glu Val Tyr Val Lys Trp Lys Phe Lys Gly Arg Asp

50 55 60

att tac acc ttt gat gga gct cta aac aag tcc act gtc ccc act gac 240

Ile Tyr Thr Phe Asp Gly Ala Leu Asn Lys Ser Thr Val Pro Thr Asp

65 70 75 80

ttt agt agt gca aaa att gaa gtc tca caa tta cta aaa gga gat gcc 288

Phe Ser Ser Ala Lys Ile Glu Val Ser Gln Leu Leu Lys Gly Asp Ala

85 90 95

tct ttg aag atg gat aag agt gat gct gtc tca cac aca gga aac tac 336

Ser Leu Lys Met Asp Lys Ser Asp Ala Val Ser His Thr Gly Asn Tyr

100 105 110

act tgt gaa gta aca gaa tta acc aga gaa ggt gaa acg atc atc gag 384

Thr Cys Glu Val Thr Glu Leu Thr Arg Glu Gly Glu Thr Ile Ile Glu

115 120 125

cta aaa tat cgt gtt gtt tca tgg ttt tct cca aat gaa aat gac tac 432

Leu Lys Tyr Arg Val Val Ser Trp Phe Ser Pro Asn Glu Asn Asp Tyr

130 135 140

aag gac gac gat gac aag tgatag 456

Lys Asp Asp Asp Asp Lys

145 150

<210>27

<211>46

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>27

ggaattccat atgcaagtgc aacttcaaca gtctggacct gaactg 46

<210>28

<211>31

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>28

ggaattctca ttattttatt tccagcttgg t 31

<210>29

<211>741

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(735)

<223>pscM2DEm02.MABL2-scFv

<400>29

atg caa gtg caa ctt caa cag tct gga cct gaa ctg gta aag cct ggg 48

Met Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly

1 5 10 15

gct tca gtg aag atg tcc tgc aag gct tct gga tac acc ttc gct aac 96

Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ala Asn

20 25 30

cat gtt att cac tgg gtg aag cag aag cca ggg cag ggc ctt gag tgg 144

His Val Ile His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp

35 40 45

att gga tat att tat cct tac aat gat ggt act aag tat aat gag aag 192

Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys

50 55 60

ttc aag gac aag gcc act ctg act tca gac aaa tcc tcc acc aca gcc 240

Phe Lys Asp Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Thr Thr Ala

65 70 75 80

tac atg gac ctc agc agc ctg gcc tct gag gac tct gcg gtc tat tac 288

Tyr Met Asp Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Tyr

85 90 95

tgt gca aga ggg ggt tac tat act tac gac gac tgg ggc caa ggc acc 336

Cys Ala Arg Gly Gly Tyr Tyr Thr Tyr Asp Asp Trp Gly Gln Gly Thr

100 105 110

act ctc aca gtc tcc tca ggt ggt ggt ggt tcg ggt ggt ggt ggt tcg 384

Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser

115 120 125

ggt ggt ggc gga tcg gat gtt gtg atg acc caa agt cca ctc tcc ctg 432

Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu

130 135 140

cct gtc agt ctt gga gat caa gcc tcc atc tct tgc aga tca agt cag 480

Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln

145 150 155 160

agc ctt gtg cac agt aat gga aag acc tat tta cat tgg tac ctg cag 528

Ser Leu Val His Ser Asn Gly Lys Thr Tyr Leu His Trp Tyr Leu Gln

165 170 175

aag cca ggc cag tct cca aaa ctc ctg atc tac aaa gtt tcc aac cga 576

Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg

180 185 190

ttt tct ggg gtc cca gac agg ttc agt ggc agt gga tca gtg aca gat 624

Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Val Thr Asp

195 200 205

ttc aca ctc atg atc agc aga gtg gag gct gag gat ctg gga gtt tat 672

Phe Thr Leu Met Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr

210 215 220

ttc tgc tct caa agt aca cat gtt ccg tac acg ttc gga ggg ggg acc 720

Phe Cys Ser Gln Ser Thr His Val Pro Tyr Thr Phe Gly Gly Gly Thr

225 230 235 240

aag ctg gaa ata aaa taatga 741

Lys Leu Glu Ile Lys

245

<210>30

<211>18

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>30

cagacagtgg ttcaaagt 18

<210>31

<211>72

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>31

cgcgtcgacc gatccgccac cacccgaacc accaccaccc gaaccaccac caccttttat 60

ttccagcttg gt 72

<210>32

<211>1605

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(1599)

<223>pCHOM2(Fv)2.MABL2-sc(Fv)2

<400>32

atg gga tgg agc tgt atc atc ctc ttc ttg gta gca aca gct aca ggt 48

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly

1 5 10 15

gtc gac tcc cag gtc cag ctg cag cag tct gga cct gaa ctg gta aag 96

Val Asp Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys

20 25 30

cct ggg gct tca gtg aag atg tcc tgc aag gct tct gga tac acc ttc 144

Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe

35 40 45

gct aac cat gtt att cac tgg gtg aag cag aag cca ggg cag ggc ctt 192

Ala Asn His Val Ile His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu

50 55 60

gag tgg att gga tat att tat cct tac aat gat ggt act aag tat aat 240

Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr Lys Tyr Asn

65 70 75 80

gag aag ttc aag gac aag gcc act ctg act tca gac aaa tcc tcc acc 288

Glu Lys Phe Lys Asp Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Thr

85 90 95

aca gcc tac atg gac ctc agc agc ctg gcc tct gag gac tct gcg gtc 336

Thr Ala Tyr Met Asp Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val

100 105 110

tat tac tgt gca aga ggg ggt tac tat act tac gac gac tgg ggc caa 384

Tyr Tyr Cys Ala Arg Gly Gly Tyr Tyr Thr Tyr Asp Asp Trp Gly Gln

115 120 125

ggc acc act ctc aca gtc tcc tca ggt ggt ggt ggt tcg ggt ggt ggt 432

Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly

130 135 140

ggt tcg ggt ggt ggc gga tcg gat gtt gtg atg acc caa agt cca ctc 480

Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Ser Pro Leu

145 150 155 160

tcc ctg cct gtc agt ctt gga gat caa gcc tcc atc tct tgc aga tca 528

Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser

165 170 175

agt cag agc ctt gtg cac agt aat gga aag acc tat tta cat tgg tac 576

Ser Gln Ser Leu Val His Ser Asn Gly Lys Thr Tyr Leu His Trp Tyr

180 185 190

ctg cag aag cca ggc cag tct cca aaa ctc ctg atc tac aaa gtt tcc 624

Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser

195 200 205

aac cga ttt tct ggg gtc cca gac agg ttc agt ggc agt gga tca gtg 672

Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Val

210 215 220

aca gat ttc aca ctc atg atc agc aga gtg gag gct gag gat ctg gga 720

Thr Asp Phe Thr Leu Met Ile Ser Arg Val Glu Ala Glu Asp Leu Gly

225 230 235 240

gtt tat ttc tgc tct caa agt aca cat gtt ccg tac acg ttc gga ggg 768

Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Tyr Thr Phe Gly Gly

245 250 255

ggg acc aag ctg gaa ata aaa ggt ggt ggt ggt tcg ggt ggt ggt ggt 816

Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly

260 265 270

tcg ggt ggt ggc gga tcg gtc gac tcc cag gtc cag ctg cag cag tct 864

Ser Gly Gly Gly Gly Ser Val Asp Ser Gln Val Gln Leu Gln Gln Ser

275 280 285

gga cct gaa ctg gta aag cct ggg gct tca gtg aag atg tcc tgc aag 912

Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys

290 295 300

gct tct gga tac acc ttc gct aac cat gtt att cac tgg gtg aag cag 960

Ala Ser Gly Tyr Thr Phe Ala Asn His Val Ile His Trp Val Lys Gln

305 310 315 320

aag cca ggg cag ggc ctt gag tgg att gga tat att tat cct tac aat 1008

Lys Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn

325 330 335

gat ggt act aag tat aat gag aag ttc aag gac aag gcc act ctg act 1056

Asp Gly Thr Lys Tyr Asn Glu Lys Phe Lys Asp Lys Ala Thr Leu Thr

340 345 350

tca gac aaa tcc tcc acc aca gcc tac atg gac ctc agc agc ctg gcc 1104

Ser Asp Lys Ser Ser Thr Thr Ala Tyr Met Asp Leu Ser Ser Leu Ala

355 360 365

tct gag gac tct gcg gtc tat tac tgt gca aga ggg ggt tac tat act 1152

Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Gly Gly Tyr Tyr Thr

370 375 380

tac gac gac tgg ggc caa ggc acc act ctc aca gtc tcc tca ggt ggt 1200

Tyr Asp Asp Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly

385 390 395 400

ggt ggt tcg ggt ggt ggt ggt tcg ggt ggt ggc gga tcg gat gtt gtg 1248

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val

405 410 415

atg acc caa agt cca ctc tcc ctg cct gtc agt ctt gga gat caa gcc 1296

Met Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala

420 425 430

tcc atc tct tgc aga tca agt cag agc ctt gtg cac agt aat gga aag 1344

Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Lys

435 440 445

acc tat tta cat tgg tac ctg cag aag cca ggc cag tct cca aaa ctc 1392

Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu

450 455 460

ctg atc tac aaa gtt tcc aac cga ttt tct ggg gtc cca gac agg ttc 1440

Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe

465 470 475 480

agt ggc agt gga tca gtg aca gat ttc aca ctc atg atc agc aga gtg 1488

Ser Gly Ser Gly Ser Val Thr Asp Phe Thr Leu Met Ile Ser Arg Val

485 490 495

gag gct gag gat ctg gga gtt tat ttc tgc tct caa agt aca cat gtt 1536

Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser Thr His Val

500 505 510

ccg tac acg ttc gga ggg ggg acc aag ctg gaa ata aaa gac tac aaa 1584

Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Asp Tyr Lys

515 520 525

gac gat gac gat aaa taatga 1605

Asp Asp Asp Asp Lys

530

<210>33

<211>23

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>33

tgaggaattc ccaccatggg atg 33

<210>34

<211>40

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>34

cacgacgtca ctcgagactg tgagagtggt gccttggccc 40

<210>35

<211>40

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>35

agtctcgagt gacgtcgtga tgacccaaag tccactctcc 40

<210>36

<211>31

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>36

gactggatcc tcattattta tcgtcatcgt c 31

<210>37

<211>22

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>37

cgcgtaatac gactcactat ag 22

<210>38

<211>46

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>38

gcaattggac ctgttttatc tcgagcttgg tcccccctcc gaacgt 46

<210>39

<211>45

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>39

gctcgagata aaacaggtcc aattgcagca gtctggacct gaact 45

<210>40

<211>60

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>40

gactggatcc tcattattta tcgtcatcgt ctttgtagtc tgaggagact gtgagagtgg 60

<210>41

<211>32

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>41

gactgaattc ccaccatgaa gttgcctgtt ag 32

<210>42

<211>40

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>42

cagtctcgag tggtggttcc gacgtcgtga tgacccaaag 40

<210>43

<211>43

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>43

cagtctcgag tggtggtggt tccgacgtcg tgatgaccca aag 43

<210>44

<211>46

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>44

cagtctcgag tggtggtggt ggttccgacg tcgtgatgac ccaaag 46

<210>45

<211>49

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>45

cagtctcgag tggtggtggt ggtggttccg acgtcgtgat gacccaaag 49

<210>46

<211>52

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>46

cagtctcgag tggtggtggt ggtggtggtt ccgacgtcgt gatgacccaa ag 52

<210>47

<211>20

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>47

ggccgcatgt tgtcacgaat 20

<210>48

<211>780

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(768)

<223>CF2HL-0/pCOS1.MABL2-scFv<HL-0>

<400>48

atg gga tgg agc tgt atc atc ctc ttc ttg gta gca aca gct aca ggt gtc 51

MET Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly Val

5 10 15

gac tcc cag gtc cag ctg cag cag tct gga cct gaa ctg gta aag cct ggg 102

Asp Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly

20 25 30

gct tca gtg aag atg tcc tgc aag gct tct gga tac acc ttc gct aac cat 153

Ala Ser Val Lys MET Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ala Asn His

35 40 45 50

gtt att cac tgg gtg aag cag aag cca ggg cag ggc ctt gag tgg att gga 204

Val Ile His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile Gly

55 60 65

tat att tat cct tac aat gat ggt act aag tat aat gag aag ttc aag gac 255

Tyr Ile Tyr Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe Lys Asp

70 75 80 85

aag gcc act ctg act tca gac aaa tcc tcc acc aca gcc tac atg gac ctc 306

Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Thr Thr Ala Tyr MET Asp Leu

90 95 100

agc agc ctg gcc tct gag gac tct gcg gtc tat tac tgt gca aga ggg ggt 357

Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Gly Gly

105 110 115

tac tat act tac gac gac tgg ggc caa ggc acc act ctc aca gtc tcg agt 408

Tyr Tyr Thr Tyr Asp Asp Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser

120 125 130 135

gac gtc gtg atg acc caa agt cca ctc tcc ctg cct gtc agt ctt gga gat 459

Asp Val Val MET Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly Asp

140 145 150

caa gcc tcc atc tct tgc aga tca agt cag agc ctt gtg cac agt aat gga 510

Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly

155 160 165 170

aag acc tat tta cat tgg tac ctg cag aag cca ggc cag tct cca aaa ctc 561

Lys Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu

175 180 185

ctg atc tac aaa gtt tcc aac cga ttt tct ggg gtc cca gac agg ttc agt 612

Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser

190 l95 200

ggc agt gga tca gtg aca gat ttc aca ctc atg atc agc aga gtg gag gct 663

Gly Ser Gly Ser Val Thr Asp Phe Thr Leu MET Ile Ser Arg Val Glu Ala

205 210 215 220

gag gat ctg gga gtt tat ttc tgc tct caa agt aca cat gtt ccg tac acg 714

Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Tyr Thr

225 230 235

ttc gga ggg ggg acc aag ctg gaa ata aaa gac tac aaa gac gat gac gat 765

Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Asp Tyr Lys Asp Asp Asp Asp

240 245 250 255

aaa taa tga gga tcc 780

Lys

<210>49

<211>45

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>49

caagctcgag ataaaatccg gaggccaggt ccaattgcag cagtc 45

<210>50

<211>48

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>50

caagctcgag ataaaatccg gaggtggcca ggtccaattg cagcagtc 48

<210>51

<211>51

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>51

caagctcgag ataaaatccg gaggtggtgg ccaggtccaa ttgcagcagt c 51

<210>52

<211>54

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>52

caagctcgag ataaaatccg gaggtggtgg tggccaggtc caattgcagc agtc 54

<210>53

<211>57

<212>DNA

＜213〉artificial sequence

<220>

＜223〉PCR primer

<400>53

caagctcgag ataaaatccg gaggtggtgg tggtggccag gtccaattgc agcagtc 57

<210>54

<2111>780

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(768)

<223>CF2LH-0/pCOS1.MABL2-scFv<LH-0>

<400>54

atg aag ttg cct gtt agg ctg ttg gtg ctg atg ttc tgg att cct ggt tcc 51

MET Lys Leu Pro Val Arg Leu Leu Val Leu MET Phe Trp Ile Pro Gly Ser

5 10 15

agc agt gat gtt gtg atg acc caa agt cca ctc tcc ctg cct gtc agt ctt 102

Ser Ser Asp Val Val MET Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu

20 25 30

gga gat caa gcc tcc atc tct tgc aga tca agt cag agc ctt gtg cac agt 153

Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser

35 40 45 50

aat gga aag acc tat tta cat tgg tac ctg cag aag cca ggc cag tct cca 204

Asn Gly Lys Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro

55 60 65

aaa ctc ctg atc tac aaa gtt tcc aac cga ttt tct ggg gtc cca gac agg 255

Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg

70 75 80 85

ttc agt ggc agt gga tca gtg aca gat ttc aca ctc atg atc agc aga gtg 306

Phe Ser Gly Ser Gly Ser Val Thr Asp Phe Thr Leu MET Ile Ser Arg Val

90 95 100

gag gct gag gat ctg gga gtt tat ttc tgc tct caa agt aca cat gtt ccg 357

Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro

105 110 115

tac acg ttc gga ggg ggg acc aag ctc gag ata aaa cag gtc caa ttg cag 408

Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gln Val Gln Leu Gln

120 125 130 135

cag tct gga cct gaa ctg gta aag cct ggg gct tca gtg aag atg tcc tgc 459

Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys MET Ser Cys

140 145 150

aag gct tct gga tac acc ttc gct aac cat gtt att cac tgg gtg aag cag 510

Lys Ala Ser Gly Tyr Thr Phe Ala Asn His Val Ile His Trp Val Lys Gln

155 160 165 170

aag cca ggg cag ggc crt gag tgg att gga tat att tat cct tac aat gat 561

Lys Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Asp

175 180 185

ggt act aag tat aat gag aag ttc aag gac aag gcc act ctg act tca gac 612

Gly Thr Lys Tyr Asn Glu Lys Phe Lys Asp Lys Ala Thr Leu Thr Ser Asp

190 195 200

aaa tcc tcc acc aca gcc tac atg gac ctc agc agc ctg gcc tct gag gac 663

Lys Ser Ser Thr Thr Ala Tyr MET Asp Leu Ser Ser Leu Ala Ser Glu Asp

205 210 215 220

tct gcg gtc tat tac tgt gca aga ggg ggt tac tat act tac gac gac tgg 714

Ser Ala Val Tyr Tyr Cys Ala Arg Gly Gly Tyr Tyr Thr Tyr Asp Asp Trp

225 230 235

ggc caa ggc acc act ctc aca gtc tcc tca gac tac aaa gac gat gac gat 765

Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Asp Tyr Lys Asp Asp Asp Asp

240 245 250 255

aaa taa tga gga tcc 780

Lys

<210>55

<211>351

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(1)...(351)

＜223〉12B5HV.1-351 peptide

<400>55

cag gtg cag ctg gtg cag tct ggg gga ggc ttg gtc cgg ccc ggg ggg 48

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Leu Val Arg Pro Gly Gly

1 5 10 15

tcc ctg agt ctc tcc tgt gca gtc tct gga atc acc ctc agg acc tac 96

Ser Leu Ser Leu Ser Cys Ala Val Ser Gly Ile Thr Leu Arg Thr Tyr

20 25 30

ggc atg cac tgg gtc cgc cag gct cca ggc aag ggg ctg gag tgg gtg 144

Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val

35 40 45

gca ggt ata tcc ttt gac gga aga agt gaa tac tat gca gac tcc gtg 192

Ala Gly Ile Ser Phe Asp Gly Arg Ser Glu Tyr Tyr Ala Asp Ser Val

50 55 60

cag ggc cga ttc acc atc tcc aga gac agt tcc aag aac acc ctg tat 240

Gln Gly Arg Phe Thr Ile Ser Arg Asp Ser Ser Lys Asn Thr Leu Tyr

65 70 75 80

ctg caa atg aac agc ctg aga gcc gag gac acg gct gtg tat tac tgt 288

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys

85 90 95

gcg aga gga gca cat tat ggt ttc gat atc tgg ggc caa ggg aca atg 336

Ala Arg Gly Ala His Tyr Gly Phe Asp Ile Trp Gly Gln Gly Thr Met

100 105 110

gtc acc gtc tcg agt 351

Val Thr Val Ser Ser

115

<210>56

<211>57

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(1)...(57)

＜223〉targeting sequencing

<400>56

atg gag ttt ggg ctg agc tgg gtt ttc ctc gtt gct ctt tta aga ggt 48

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Leu Arg Gly

5 10 15

gtc cag tgt 57

Val Gln Cys

<210>57

<211>115

<212>DNA

＜213〉artificial sequence

<220>

<223>12B5VH-1

<400>57

atggagtttg ggctgagctg ggttttcctc gttgctcttt taagaggtgt ccagtgtcag 60

gtgcagctgg tgcagtctgg gggaggcttg gtccggcccg gggggtccct gagtc 115

<210>58

<211>115

<212>DNA

＜213〉artificial sequence

<220>

<223>12B5VH-2

<400>58

aaggatatac ctgccaccca ctccagcccc ttgcctggag cctggcggac ccagtgcatg 60

ccgtaggtcc tgagggtgat tccagagact gcacaggaga gactcaggga ccccc 115

<210>59

<211>115

<212>DNA

＜213〉artificial sequence

<220>

<223>12B5VH-3

<400>59

ggcaggtata tcctttgacg gaagaagtga atactatgca gactccgtgc agggccgatt 60

caccatctcc agagacagtt ccaagaacac cctgtatctg caaatgaaca gcctg 115

<210>60

<211>108

<212>DNA

＜213〉artificial sequence

<220>

<223>12B5VH-4

<400>60

actcgagacg gtgaccattg tcccttggcc ccagatatcg aaaccataat gtgctcctct 60

cgcacagtaa tacacagccg tgtcctcggc tctcaggctg ttcatttg 108

<210>61

<211>32

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12B5VH-S, the PCR primer

<400>61

ttcaagcttc caccatggag tttgggctga gc 32

<210>62

<211>34

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12B5VH-A, the PCR primer

<400>62

ttgggatcca ctcaccactc gagacggtga ccat 34

<210>63

<211>588

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(236)...(558)

＜223〉1-235; Intron, 236-558; Human IgG constant region (part)

<400>63

gaattcgtga gtggatccca agctagcttt ctggggcagg ccaggcctga ccttggcttt 60

ggggcaggga gggggctaag gtgaggcagg tggcgccagc caggtgcaca cccaatgccc 120

atgagcccag acactggacg ctgaacctcg cggacagtta agaacccagg ggcctctgcg 180

ccctgggccc agctctgtcc cacaccgcgg tcacatggca caacctctct tgca gcc 237

Ala

1

tcc acc aag ggc cca tcg gtc ttc ccc ctg gca ccc tcc tcc aag agc 285

Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser

5 10 15

acc tct ggg ggc aca gcg gcc ctg ggc tgc ctg gtc aag gac tac ttc 333

Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe

20 25 30

ccc gaa ccg gtg acg gtg tcg tgg aac tca ggc gcc ctg acc agc ggc 381

Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly

35 40 45

gtg cac acc ttc ccg gct gtc cta cag tcc tca gga ctc tac tcc ctc 429

Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu

50 55 60 65

agc agc gtg gtg acc gtg ccc tcc agc agc ttg ggc acc cag acc tac 477

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr

70 75 80

atc tgc aacgtg aat cac aag ccc agc aac acc aag gtg gac aag aaa 525

Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys

85 90 95

gtt gag ccc aaa tct tgt gac aaa act cac aca 558

Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr

100 105

<210>64

<211>27

<212>DNA

＜213〉artificial sequence

<220>

＜223〉G1CH1-S, the PCR primer

<400>64

tgagaattcg tgagtggatc ccaagct 27

<210>65

<211>60

<212>DNA

＜213〉artificial sequence

<220>

＜223〉G1CH1-A, the PCR primer

<400>65

aaaagatctt tatcatgtgt gagttttgtc acaagatttg ggctcaactt tcttgtccac 60

<210>66

<211>432

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(12)...(419)

＜223〉HEF-12B5H-g gamma.12-419 peptide

<400>66

aagcttccac c atg gag ttt ggg ctg agc tgg gtt ttc ctc gtt gct ctt 50

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu

1 5 10

tta aga ggt gtc cag tgt cag gtg cag ctg gtg cag tct ggg gga ggc 98

Leu Arg Gly yal Gln Cys Gln Val Gln Leu Val Gln Ser Gly Gly Gly

15 20 25

ttg gtc cgg ccc ggg ggg tcc ctg agt ctc tcc tgt gca gtc tct gga 146

Leu Val Arg Pro Gly Gly Ser Leu Ser Leu Ser Cys Ala Val Ser Gly

30 35 40 45

atc acc ctc agg acc tac ggc atg cac tgg gtc cgc cag gct cca ggc 194

Ile Thr Leu Arg Thr Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly

50 55 60

aag ggg ctg gag tgg gtg gca ggt ata tcc ttt gac gga aga agt gaa 242

Lys Gly Leu Glu Trp Val Ala Gly Ile Ser Phe Asp Gly Arg Ser Glu

65 70 75

tac tat gca gac tcc gtg cag ggc cga ttc acc atc tcc aga gac agt 290

Tyr Tyr Ala Asp Ser Val Gln Gly Arg Phe Thr Ile Ser Arg Asp Ser

80 85 90

tcc aag aac acc ctg tat ctg caa atg aac agc ctg aga gcc gag gac 338

Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp

95 100 105

acg gct gtg tat tac tgt gcg aga gga gca cat tat ggt ttc gat atc 386

Thr Ala Val Tyr Tyr Cys Ala Arg Gly Ala His Tyr Gly Phe Asp Ile

110 115 120 125

tgg ggc caa ggg aca atg gtc acc gtc tcg agt ggtgagtgga tcc 432

Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser

130 135

<210>67

<211>321

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(1)...(321)

＜223〉12B5LV.1-321 peptide

<400>67

gac atc cag atg acc cag tct cct tcc acc ctg tct gca tct att gga 48

Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Ile Gly

1 5 10 15

gac aga gtc acc atc acc tgc cgg gcc agc gag ggt att tat cac tgg 96

Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Gly Ile Tyr His Trp

20 25 30

ttg gcc tgg tat cag cag aag cca ggg aaa gcc cct aaa ctc ctg atc 144

Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile

35 40 45

tat aag gcc tct agt tta gcc agt ggg gcc cca tca agg ttc agc ggc 192

Tyr Lys Ala Ser Ser Leu Ala Ser Gly Ala Pro Ser Arg Phe Ser Gly

50 55 60

agt gga tct ggg aca gat ttc act ctc acc atc agc agc ctg cag cct 240

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro

65 70 75 80

gat gat ttt gca act tat tac tgc caa caa tat agt aat tat ccg ctc 288

Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Asn Tyr Pro Leu

85 90 95

act ttc ggc gga ggg acc aag ctg gag atc aaa 321

Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys

100 105

<210>68

<211>66

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(1)...(66)

＜223〉targeting sequencing

<400>68

atg gac atg agg gtc ccc gct cag ctc ctg ggg ctc ctg ctg ctc tgg 48

MET Asp MET Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp

5 10 15

ctc cca ggt gcc aaa tgt 66

Leu Pro Gly Ala Lys Cys

20

<210>69

<211>110

<212>DNA

＜213〉artificial sequence

<220>

<223>12B5VL-1

<400>69

atggacatga gggtccccgc tcagctcctg gggctcctgc tgctctggct cccaggtgcc 60

aaatgtgaca tccagatgac ccagtctcct tccaccctgt ctgcatctat 110

<210>70

<211>110

<212>DNA

＜213〉artificial sequence

<220>

<223>12B5VL-2

<400>70

ggagtttagg ggctttccct ggcttctgct gataccaggc caaccagtga taaataccct 60

cgctggcccg gcaggtgatg gtgactctgt ctccaataga tgcagacagg 110

<210>71

<211>110

<212>DNA

＜213〉artificial sequence

<220>

<223>12B5VL-3

<400>71

aagcccctaa actcctgatc tataaggcct ctagtttagc cagtggggcc ccatcaaggt 60

tcagcggcag tggatctggg acagatttca ctctcaccat cagcagcctg 110

<210>72

<211>103

<212>DNA

＜213〉artificial sequence

<220>

<223>12B5VL-4

<400>72

tttgatctcc agcttggtcc ctccgccgaa agtgagcgga taattactat attgttggca 60

gtaataagtt gcaaaatcaat caggctgcag gctgctgatg gtg 103

<210>73

<211>32

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12B5VL-S, the PCR primer

<400>73

ttcaagcttc caccatggac atgagggtcc cc 32

<210>74

<211>35

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12B5VL-A, the PCR primer

<400>74

tctaggatcc actcacgttt gatctccagc ttggt 35

<210>75

<211>415

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(12)...(398)

＜223〉HEF-12B5H-g kappa.12-398 peptide

<400>75

aagcttccac c atg gac atg agg gtc ccc gct cag ctc ctg ggg ctc ctg 50

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu

1 5 10

ctg ctc tgg ctc cca ggt gcc aaa tgt gac atc cag atg acc cag tct 98

Leu Leu Trp Leu Pro Gly Ala Lys Cys Asp Ile Gln Met Thr Gln Ser

15 20 25

cct tcc acc ctg tct gca tct att gga gac aga gtc acc atc acc tgc 146

Pro Ser Thr Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr Cys

30 35 40 45

cgg gcc agc gag ggt att tat cac tgg ttg gcc tgg tat cag cag aag 194

Arg Ala Ser Glu Gly Ile Tyr His Trp Leu Ala Trp Tyr Gln Gln Lys

50 55 60

cca ggg aaa gcc cct aaa ctc ctg atc tat aag gcc tct agt tta gcc 242

Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Lys Ala Ser Ser Leu Ala

65 70 75

agt ggg gcc cca tca agg ttc agc ggc agt gga tct ggg aca gat ttc 290

Ser Gly Ala Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe

80 85 90

act ctc acc atc agc agc ctg cag cct gat gat ttt gca act tat tac 338

Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr

95 100 105

tgc caa caa tat agt aat tat ccg ctc act ttc ggc gga ggg acc aag 386

Cys Gln Gln Tyr Ser Asn Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys

110 115 120 125

ctg gag atc aaa cgtgagtgga tcctaga 415

Leu Glu Ile Lys

<210>76

<211>24

<212>DNA

＜213〉artificial sequence

<220>

＜223〉FLAG flag sequence

<400>76

gac tac aag gat gac gac gat aag 24

Asp Tyr Lys Asp Asp Asp Asp Lys

5

<210>77

<211>31

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12B5-S, the PCR primer

<400>77

atagaattcc accatggagt ttgggctgag c 31

<210>78

<211>24

<212>DNA

＜213〉artificial sequence

<220>

＜223〉HuVHJ3, the PCR primer

<400>78

tgaagagacg gtgaccattg tccc 24

<210>79

<211>28

<212>DNA

＜213〉artificial sequence

<220>

＜223〉RhuJH3, the PCR primer

<400>79

ggacaatggt caccgtctct tcaggtgg 28

<210>80

<211>32

<212>DNA

＜213〉artificial sequence

<220>

＜223〉RhuVK1, the PCR primer

<400>80

ggagactggg tcatctggat gtccgatccg cc 32

<210>81

<211>23

<212>DNA

＜213〉artificial sequence

<220>

＜223〉HuVK1.2, the PCR primer

<400>81

gacatccaga tgacccagtc tcc 23

<210>82

<211>59

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12B5F-A, the PCR primer

<400>82

attgcggccg cttatcactt atcgtcgtca tccttgtagt ctttgatctc cagcttggt 59

<210>83

<211>45

<212>DNA

＜213〉artificial sequence

<220>

＜223〉joint aminoacid sequence and nucleotide sequence

<400>83

ggt ggt ggt ggt tcg ggt ggt ggt ggt tcg ggt ggt ggc gga tcg 45

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser

5 10 15

<210>84

<211>823

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(12)...(809)

＜223〉sc12B5, strand Fv

<400>84

aagcttccac c atg gag ttt ggg ctg agc tgg gtt ttc crc gtt gct ctt 50

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu

1 5 10

tta aga ggt gtc cag tgt cag gtg cag ctg gtg cag tct ggg gga ggc 98

Leu Arg Gly Val Gln Cys Gln Val Gln Leu Val Gln Ser Gly Gly Gly

15 20 25

ttg gtc cgg ccc ggg ggg tcc ctg agt ctc tcc tgt gca gtc tct gga 146

Leu Val Arg Pro Gly Gly Ser Leu Ser Leu Ser Cys Ala Val Ser Gly

30 35 40 45

atc acc ctc agg acc tac ggc atg cac tgg gtc cgc cag gct cca ggc 194

Ile Thr Leu Arg Thr Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly

50 55 60

aag ggg ctg gag tgg gtg gca ggt ata tcc ttt gac gga aga agt gaa 242

Lys Gly Leu Glu Trp Val Ala Gly Ile Ser Phe Asp Gly Arg Ser Glu

65 70 75

tac tat gca gac tcc gtg cag ggc cga ttc acc atc tcc aga gac agt 290

Tyr Tyr Ala Asp Ser Val Gln Gly Arg Phe Thr Ile Ser Arg Asp Ser

80 85 90

tcc aag aac acc ctg tat ctg caa atg aac agc ctg aga gcc gag gac 338

Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp

95 100 105

acg gct gtg tat tac tgt gcg aga gga gca cat tat ggt ttc gat atc 386

Thr Ala Val Tyr Tyr Cys Ala Arg Gly Ala His Tyr Gly Phe Asp Ile

110 115 120 125

tgg ggc caa ggg aca atg gtc acc gtc tcg agt ggt ggt ggt ggt tcg 434

Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser

130 135 140

ggt ggt ggt ggt tcg ggt ggt ggc gga tcg gac atc cag atg acc cag 482

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln

145 150 155

tct cct tcc acc ctg tct gca tct att gga gac aga gtc acc atc acc 530

Ser Pro Ser Thr Leu Ser Ala Ser Ile Gly Asp Arg Val Thr Ile Thr

160 165 170

tgc cgg gcc agc gag ggt att tat cac tgg ttg gcc tgg tat cag cag 578

Cys Arg Ala Ser Glu Gly Ile Tyr His Trp Leu Ala Trp Tyr Gln Gln

175 180 185

aag cca ggg aaa gcc cct aaa ctc ctg atc tat aag gcc tct agt tta 626

Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Lys Ala Ser Ser Leu

190 195 200 205

gcc agt ggg gcc cca tca agg ttc agc ggc agt gga tct ggg aca gat 674

Ala Ser Gly Ala Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp

210 215 220

ttc act ctc acc atc agc agc ctg cag cct gat gat ttt gca act tat 722

Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr

225 230 235

TAC TGC CAA CAA TAT AGT AAT TAT CCG CTC ACT TTC GGC GGA GGG ACC 770

Tyr Cys Gln Gln Tyr Ser Asn Tyr Pro Leu Thr Phe Gly Gly Gly Thr

240 245 250

aag ctg gag atc aaa gac tac aag gat gac gac gat aag tgataagcgg c 820

Lys Leu Glu Ile Lys Asp Tyr Lys Asp Asp Asp Asp Lys

255 260 265

cgc 823

<210>85

<211>114

<212>PRT

＜213〉people

<400>85

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Glu

1 5 10 15

Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser Ile Ser Ser Tyr

20 25 30

Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys

50 55 60

Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Ser Gln Phe Ser Leu

65 70 75 80

Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala

85 90 95

Arg Gly Arg Tyr Phe Asp Val Trp Gly Arg Gly Thr Met Val Thr Val

100 105 110

Ser Ser

<210>86

<211>342

<212>DNA

＜213〉people

<400>86

caggtgcagc tgcagcagtc gggcccagga ctggtgaagc cttcggagac cctgtccctc 60

acctgcactg tctctggtga ctccatcagt agttactact ggagctggat tcggcagccc 120

ccagggaagg gactggagtg gattgggtat atctattaca gtgggagcac caactacaac 180

ccctccctca agagtcgagt caccatatca gtagacacgt ccaagagcca gttctccctg 240

aagctgagct ctgtgaccgc cgcagacacg gccgtgtatt actgtgcgag agggcggtac 300

ttcgatgtct ggggccgtgg caccatggtc actgtctcct ca 342

<210>87

<211>57

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(1)...(57)

＜223〉targeting sequencing

<308>GenBank No.AF062252

<400>87

atg aaa cat ctg tgg ttc ttc ctt ctc ctg gtg gca gct ccc aga tgg 48

Met Lys His Leu Trp Phe Phe Leu Leu Leu Val Ala Ala Pro Arg Trp

l 5 10 15

gtc ctg tcc 57

Val Leu Ser

<210>88

<211>110

<212>DNA

＜213〉artificial sequence

<220>

<223>12E10Vtt1

<400>88

atgaaacatc tgtggttctt ccttctcctg gtggcagctc ccagatgggt cctgtcccag 60

gtgcagctgc agcagtcggg cccaggactg gtgaagcctt cggagaccct 110

<210>89

<211>110

<212>DNA

＜213〉artificial sequence

<220>

<223>12E10VH2

<400>89

acccaatcca ctccagtccc ttccctgggg gctgccgaat ccagctccag tagtaactac 60

tgatggagtc accagagaca gtgcaggtga gggacagggt ctccgaaggc 110

<210>90

<211>110

<212>DNA

＜213〉artificial sequence

<220>

<223>12E10VH3

<400>90

tggagtggat tgggtatatc tattacagtg ggagcaccaa ctacaacccc tccctcaaga 60

gtcgagtcac catatcagta gacacgtcca agagccagtt ctccctgaag 110

<210>91

<211>114

<212>DNA

＜213〉artificial sequence

<220>

<223>12E10VH4

<400>91

tgaggagaaca gtgaccatgg tgccacggcc ccagacatcg aagtaccgcc ctctcgcaca 60

gtaatacaacg gccgtgtctg cggcggtcac agagctcagc ttcagggaga actg 114

<210>92

<211>32

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10VHS, the PCR primer

<400>92

ttcaagcttc caccatgaaa catctgtggt tc 32

<210>93

<211>34

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10VHA, the PCR primer

<400>93

ttgggatcca ctcacctgag gagacagtga ccat 34

<210>94

<211>426

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(12)...(417)

＜223〉12E10H, H chain V district

<400>94

aagcttccac c atg aaa cat ctg tgg ttc ttc ctt ctc ctg gtg gca gct 50

Met Lys His Leu Trp Phe Phe Leu Leu Leu Val Ala Ala

1 5 10

ccc aga tgg gtc ctg tcc cag gtg cag ctg cag cag tcg ggc cca gga 98

Pro Arg Trp Val Leu Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Gly

15 20 25

ctg gtg aag cct tcg gag acc ctg tcc ctc acc tgc act gtc tct ggt 146

Leu Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Thr Val Ser Gly

30 35 40 45

gac tcc atc agt agt tac tac tgg agc tgg att cgg cag ccc cca ggg 194

Asp Ser Ile Ser Ser Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly

50 55 60

aag gga ctg gag tgg att ggg tat atc tat tac agt ggg agc acc aac 242

Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn

65 70 75

tac aac ccc tcc ctc aag agt cga gtc acc ata tca gta gac acg tcc 290

Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser

80 85 90

aag agc cag ttc tcc ctg aag ctg agc tct gtg acc gcc gca gac acg 338

Lys Ser Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr

95 100 105

gcc gtg tat tac tgt gcg aga ggg cgg tac ttc gat gtc tgg ggc cgt 386

Ala Val Tyr Tyr Cys Ala Arg Gly Arg Tyr Phe Asp Val Trp Gly Arg

110 115 120 125

ggc acc atg gtc act gtc tcc tca ggtgagtgga tcccaa 426

Gly Thr Met Val Thr Val Ser Ser

130

<210>95

<211>110

<212>PRT

<213>Mus

<400>95

Ser Tyr Val Leu Thr Gln Pro Pro Ser Val Ser Gly Ser Pro Gly Gln

1 5 10 15

Ser Ile Thr Ile Ser Cys Thr Gly Thr Ser Ser Asp Val Gly Gly Tyr

20 25 30

Asn Tyr Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu

35 40 45

Met Ile Tyr Glu Gly Ser Lys Arg Pro Ser Gly Val Ser Asn Arg Phe

50 55 60

Ser Gly Ser Lys Ser Gly Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu

65 70 75 80

Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Tyr Thr Thr Arg

85 90 95

Ser Thr Arg Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu

100 105 110

<210>96

<211>330

<212>DNA

<213>Mus

<400>96

tcctatgtgc tgactcagcc accctcggtg tcagggtctc ctggacagtc gatcaccatc 60

tcctgcactg gaaccagcag tgacgttggt ggttataact atgtctcctg gtaccaacag 120

cacccaggca aagcccccaa actcatgatt tatgagggca gtaaacggcc ctcaggggtt 180

tctaatcgct tctctggctc caagtctggc aacacggcct ccctgaccat ctctgggctc 240

caggctgagg acgaggctga ttattactgc agctcatata caaccagaag cactcgggtg 300

ttcggcggag ggaccaagct gaccgtccta 330

<210>97

<211>57

<212>DNA

＜213〉people

<220>

<221>CDS

<222>(1)...(57)

＜223〉targeting sequencing

<310>

<400>97

atg gcc tgg acc gtt ctc ctc ctc ggc ctc ctc tct cac tgc aca ggc 48

Met Ala Trp Thr Val Leu Leu Leu Gly Leu Leu Ser His Cys Thr Gly

1 5 10 15

tct gtg acc 57

Ser Val Thr

<210>98

<211>110

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10VL1, the PCR primer

<400>98

atggcctgga ccgttctcct cctcggcctc ctctctcact gcacaggctc tgtgacctcc 60

tatgtgctga ctcagccacc ctcggtgtca gggtctcctg gacagtcgat 110

<210>99

<211>62

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10VL2, the PCR primer

<400>99

tcatgagttt gggggctttg cctgggtgct gttggtacca ggagacatag ttataaccac 60

caacgtcact gctggttcca gtgcaggaga tggtgatcga ctgtccagga 110

<210>100

<211>110

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10VL3, the PCR primer

<400>100

cccccaaact catgatttat gagggcagta aacggccctc aggggtttct aatcgcttct 60

ctggctccaa gtctggcaac acggcctccc tgaccatctc tgggctccag 110

<210>101

<211>102

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10VL4, the PCR primer

<400>101

taggacggtc agcttggtcc ctccgccgaa cacccgagtg cttctggttg tatatgagct 60

gcagtaataa tcagcctcgt cctcagcctg gagcccagag at 102

<210>102

<211>31

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10VLS, the PCR primer

<400>102

atcaagcttc caccatggcc tggaccgttc t 31

<210>103

<211>36

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10VLA, the PCR primer

<400>103

ctaggatccg ggctgaccta ggacggtcag cttggt 36

<210>104

<211>387

<212>DNA

<213>Mus

<220>

<221>CDS

<222>(1)...(387)

＜223〉12E10L, L chain V district

<310>

<400>104

atg gcc tgg acc gtt ctc ctc ctc ggc ctc ctc tct cac tgc aca ggc 48

Met Ala Trp Thr Val Leu Leu Leu Gly Leu Leu Ser His Cys Thr Gly

1 5 10 15

tct gtg acc tcc tat gtg ctg act cag cca ccc tcg gtg tca ggg tct 96

Ser Val Thr Ser Tyr Val Leu Thr Gln Pro Pro Ser Val Ser Gly Ser

20 25 30

cct gga cag tcg atc acc atc tcc tgc act gga acc agc agt gac gtt 144

Pro Gly Gln Ser Ile Thr Ile Ser Cys Thr Gly Thr Ser Ser Asp Val

35 40 45

ggt ggt tat aac tat gtc tcc tgg tac caa cag cac cca ggc aaa gcc 192

Gly Gly Tyr Asn Tyr Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala

50 55 60

ccc aaa ctc atg att tat gag ggc agt aaa cgg ccc tca ggg gtt tct 240

Pro Lys Leu Met Ile Tyr Glu Gly Ser Lys Arg Pro Ser Gly Val Ser

65 70 75 80

aat cgc ttc tct ggc tcc aag tct ggc aac acg gcc tcc ctg acc atc 288

Asn Arg Phe Ser Gly Ser Lys Ser Gly Asr Thr Ala Ser Leu Thr Ile

85 90 95

tct ggg ctc cag gct gag gac gag gct gat tat tac tgc agc tca tat 336

Ser Gly Leu Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Tyr

100 105 110

Aca acc aga agc act cgg gtg ttc ggc gga ggg acc aag ctg acc gtc 384

Thr Thr Arg Ser Thr Arg Val Phe Gly Gly Gly Thr Lys Leu Thr Val

115 120 125

cta 387

Leu

<210>105

<211>24

<212>DNA

＜213〉artificial sequence

<220>

<221>CDS

<222>(1)...(24)

＜223〉FLAG, targeting sequencing

<400>105

gac tac aag gat gac gac gat aag 24

Asp Tyr Lys Asp Asp Asp Asp Lys

<210>106

<211>30

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10S, the PCR primer

<400>106

tatgaattcc accatgaaac atctgtggtt 30

<210>107

<211>38

<212>DNA

＜213〉artificial sequence

<220>

＜223〉DB2, the PCR primer

<400>107

taggagctac cgcctccacc tgaggagaca gtgaccat 38

<210>108

<211>44

<212>DNA

＜213〉artificial sequence

<220>

＜223〉DB1, the PCR primer

<400>108

gtctcctcag gtggaggcgg tagctcctat gtgctgactc agcc 44

<210>109

<211>59

<212>DNA

＜213〉artificial sequence

<220>

＜223〉12E10FA, the PCR primer

<400>109

attgcggccg cttatcactt atcgtcgtca tccttgtagt ctaggacggt cagcttggt 59

<210>110

<211>792

<212>DNA

＜213〉artificial sequence

<220>

<221>CDS

<222>(11)...(778)

＜223〉12E10, strand Fv

<400>110

gaattccacc atg aaa cat ctg tgg ttc ttc ctt ctc ctg gtg gca gct 49

Met Lys His Leu Trp Phe Phe Leu Leu Leu Val Ala Ala

1 5 10

ccc aga tgg gtc ctg tcc cag gtg cag ctg cag cag tcg ggc cca gga 97

Pro Arg Trp Val Leu Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Gly

15 20 25

ctg gtg aag cct tcg gag acc ctg tcc ctc acc tgc act gtc tct ggt 145

Leu Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Thr Val Ser Gly

30 35 40 45

gac tcc atc agt agt tac tac tgg agc tgg att cgg cag ccc cca ggg 193

Asp Ser Ile Ser Ser Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly

50 55 60

aag gga ctg gag tgg att ggg tat atc tat tac agt ggg agc acc aac 241

Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn

65 70 75

tac aac ccc tcc ctc aag agt cga gtc acc ata tca gta gac acg tcc 289

Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser

80 85 90

aag agc cag ttc tcc ctg aag ctg agc tct gtg acc gcc gca gac acg 337

Lys Ser Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr

95 100 105

gcc gtg tat tac tgt gcg aga ggg cgg tac ttc gat gtc tgg ggc cgt 385

Ala Val Tyr Tyr Cys Ala Arg Gly Arg Tyr Phe Asp Val Trp Gly Arg

110 115 120 125

ggc acc atg gtc act gtc tcc tca ggt gga ggc ggt agc tcc tat gtg 433

Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Ser Tyr Val

130 135 140

ctg act cag cca ccc tcg gtg tca ggg tct cct gga cag tcg atc acc 481

Leu Thr Gln Pro Pro Ser Val Ser Gly Ser Pro Gly Gln Ser Ile Thr

145 150 155

atc tcc tgc act gga acc agc agt gac gtt ggt ggt tat aac tat gtc 529

Ile Ser Cys Thr Gly Thr Ser Ser Asp Val Gly Gly Tyr Asn Tyr Val

160 165 170

tcc tgg tac caa cag cac cca ggc aaa gcc ccc aaa ctc atg att tat 577

Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu Met Ile Tyr

175 180 185

gag ggc agt aaa cgg ccc tca ggg gtt tct aat cgc ttc tct ggc tcc 625

Glu Gly Ser Lys Arg Pro Ser Gly Val Ser Asn Arg Phe Ser Gly Ser

190 195 200 205

aag tct ggc aac acg gcc tcc ctg acc atc tct ggg ctc cag gct gag 673

Lys Ser Gly Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu Gln Ala Glu

210 215 220

gac gag gct gat tat tac tgc agc tca tat aca acc aga agc act cgg 721

Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Tyr Thr Thr Arg Ser Thr Arg

225 230 235

gtg ttc ggc gga ggg acc aag ctg acc gtc cta gac tac aag gat gac 769

Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Asp Tyr Lys Asp Asp

240 245 250

gac gat aag tgataagcgg ccgc 792

Asp Asp Lys

255

<210>111

<211>62

<212>DNA

＜213〉artificial sequence

<220>

＜223〉sc4.3, the PCR primer

<400>111

ggtggctgag tcagcacata ggacgatccg ccaccacccg aaccaccacc acccgaacca 60

cc 62

<210>112

<211>61

<212>DNA

＜213〉artificial sequence

<220>

＜223〉sc1.3, the PCR primer

<400>112

gcaccatggt cactgtctcc tcaggtggtg gtggttcggg tggtggtggt tcgggtggtg 60

g 61

<210>113

<211>822

<212>DNA

＜213〉artificial sequence

<220>

<221>CDS

<222>(11)...(807)

＜223〉sc12E10, strand Fv

<400>113

gaattccacc atg aaa cat ctg tgg ttc ttc ctt ctc ctg gtg gca gct 49

Met Lys His Leu Trp Phe Phe Leu Leu Leu Val Ala Ala

1 5 10

ccc aga tgg gtc ctg tcc cag gtg cag ctg cag cag tcg ggc cca gga 97

Pro Arg Trp Val Leu Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Gly

15 20 25

ctg gtg aag cct tcg gag acc ctg tcc ctc acc tgc act gtc tct ggt 145

Leu Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Thr Val Ser Gly

30 35 40 45

gac tcc atc agt agt tac tac tgg agc tgg att cgg cag ccc cca ggg 193

Asp Ser Ile Ser Ser Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly

50 55 60

aag gga ctg gag tgg att ggg tat atc tat tac agt ggg agc acc aac 241

Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn

65 70 75

tac aac ccc tcc ctc aag agt cga gtc acc ata tca gta gac acg tcc 289

Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser

80 85 90

aag agc cag ttc tcc ctg aag ctg agc tct gtg acc gcc gca gac acg 337

Lys Ser Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr

95 100 105

gcc gtg tat tac tgt gcg aga ggg cgg tac ttc gat gtc tgg ggc cgt 385

Ala Val Tyr Tyr Cys Ala Arg Gly Arg Tyr Phe Asp Val Trp Gly Arg

110 115 120 125

ggc acc atg gtc act gtc tcc tca ggt ggt ggt ggt tcg ggt ggt ggt 433

Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly

130 135 140

ggt tcg ggt ggt ggc gga tcg tcc tat gtg ctg act cag cca ccc tcg 481

Gly Ser Gly Gly Gly Gly Ser Ser Tyr Val Leu Thr Gln Pro Pro Ser

145 150 155

gtg tca ggg tct cct gga cag tcg atc acc atc tcc tgc act gga acc 529

Val Ser Gly Ser Pro Gly Gln Ser Ile Thr Ile Ser Cys Thr Gly Thr

160 165 170

agc agt gac gtt ggt ggt tat aac tat gtc tcc tggtac caa cag cac 577

Ser Ser Asp Val Gly Gly Tyr Asn Tyr Val Ser Trp Tyr Gln Gln His

175 180 185

cca ggc aaa gcc ccc aaa ctc atg att tat gag ggc agt aaa cgg ccc 625

Pro Gly Lys Ala Pro Lys Leu Met Ile Tyr Glu Gly Ser Lys Arg Pro

190 195 200 205

tca ggg gtt tct aat cgc ttc tct ggc tcc aag tct ggc aac acg gcc 673

Ser Gly Val Ser Asn Arg Phe Ser Gly Ser Lys Ser Gly Asn Thr Ala

210 215 220

tcc ctg acc atc tct ggg ctc cag gct gag gac gag gct gat tat tac 721

Ser Leu Thr Ile Ser Gly Leu Gln Ala Glu Asp Glu Ala Asp Tyr Tyr

225 230 235

tgc agc tca tat aca acc aga agc act cgg gtg ttc ggc gga ggg acc 769

Cys Ser Ser Tyr Thr Thr Arg Ser Thr Arg Val Phe Gly Gly Gly Thr

240 245 250

aag ctg acc gtc cta gac tac aag gat gac gac gat aag tgataagcgg 818

Lys Leu Thr Val Leu Asp Tyr Lys Asp Asp Asp Asp Lys

255 260 265

ccgc 822

Claims (16)

1. increase the method for agonist effect, it is undertaken by the antibody that antibody is changed over change, the antibody of described change comprises two or more H chain V districts and two or more L chains V district of this antibody, and with molecule in crosslinked cell surface molecule or the cell, the antibody of wherein said change is:

(a) comprise the dimer of the strand Fv in H chain V district and L chain V district; Or

(b) comprise the single chain polypeptide in two H chain V districts and two L chain V districts,

And the antibody of wherein said change has higher agonist activity than parental antibody.

2. the process of claim 1 wherein that H chain V district links to each other by joint with L chain V district.

3. the method for claim 2, wherein this joint is to comprise at least one amino acid whose peptide linker.

4. each method of claim 1-3, wherein the antibody that should change is made up of the tetramer, trimer or the dimer of strand Fv.

5. the method for claim 4 wherein is somebody's turn to do the antibody that changes and is made up of the dimer of strand Fv.

6. the process of claim 1 wherein that the antibody of this change comprises the aminoacid sequence that is used for peptide purification in addition.

7. the process of claim 1 wherein that the antibody of this change is purified.

8. the process of claim 1 wherein that H chain V district and/or L chain V district are H chain V district and/or the L chain V districts that derives from people's antibody.

9. the process of claim 1 wherein that H chain V district and/or L chain V district are humanized H chain V district and/or L chain V district.

10. the process of claim 1 wherein that the interior molecule of this cell surface molecule or cell is hormone receptor, cytokine receptor, tyrosine kinase receptor or nucleus inner recipient.

11. the process of claim 1 wherein that the interior molecule of this cell surface molecule or cell is an erythropoietin receptor, granulocyte colony stimulating factor receptor, macrophage colony-stimulating factor receptor, the granulocyte macrophage colony stimulating factor receptor, Tumor Necrosis Factor Receptors, interleukin 1 receptor, interleukin 2 receptor, the interleukin 3 receptor, Nuvance, the interleukin 5 receptor, interleukin-6 receptor, the interleukin 7 receptor, the interleukin 9 receptor, the interleukin 10 receptor, the interleukin 11 receptor, the interleukin 12 receptor, the Interleukin-13 receptor, the Interleukin-15 receptor, the interferon-' alpha ' receptor, the interferon-beta receptor, the interferon-receptor, growth hormone receptor, Insulin receptor INSR, blood stem cell multiplicaiton factor receptor, vascular endothelial growth factor receptor, the epithelial cell growth factor receptor 2 body, trk C, fibroblast growth factor acceptor, the platelet-derived growth factor receptor, transform growth factor-beta receptor, the leukocyte migration inhibition factor receptor, cntf receptor, the oncostatin M receptor, the Notch family receptors, E2F, E2F/DP1 or TAK1/TAB1.

12. the process of claim 1 wherein that this agonist act as apoptosis-induced, inducing cell propagation, inducing cell differentiation, inducing cell division or Cycle Regulation effect.

13. the process of claim 1 wherein that the antibody of this change is the antibody that monospecific changes.

14. the process of claim 1 wherein that the antibody of this change is the antibody of the change of polyspecific.

15. the method for claim 14, wherein the antibody of this change is the antibody of the change of bispecific.

16. the method for claim 15, wherein this L chain V district and H chain V district are from same monoclonal antibody.

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