Summary of the invention: the object of the present invention is to provide a kind of preparation method of ganoderma lucidum chewable tablet and the product that obtains thereof, the present invention makes the alcohol-insoluble substances post precipitation with Ganoderma extract with ethanol, the ganoderma lucidum triterpene material with bitterness that obtains pure dissolubility is Ganodenic acid, Ganoderma lucidum (Leyss. Ex Fr.) Karst. acid, Ganoderma lucidum (Leyss. Ex Fr.) Karst. ketone, red clever acid etc., these material reuse beta-cyclodextrin inclusion compounds, get the enclose powder, add alcohol-insoluble substances precipitation and adjuvant, make chewable tablet.Solving the material Ganoderma has bitterness, patient and is reluctant oral problem; Directly be prepared into chewable tablet or buccal tablet, good, the taking convenience of mouthfeel not only, and can mind tranquilizing and the heart calming, invigorating the spleen and regulating the stomach, to insomnia forgetfulness, physical weakness, diseases such as neurasthenia have curative effect preferably, chewable tablet quality stability, mouldability have been improved, medicine is by chewing or containing, and its effective ingredient through port transmucosal or hypoglossis mucous membrane absorb rapidly, absorbs fast, the bioavailability height is to solve the problem that prior art exists.
Ganoderma lucidum chewable tablet of the present invention is preparation like this: get Ganoderma 3000mg, decoct with water 1~3 time, each 1~5 hour, collecting decoction, filter, filtrate is condensed into thick paste, adds ethanol and makes precipitation fully, and precipitation reuse second ferment cleans 1-3 time, precipitation is dry below 70 ℃, be ground into fine powder, get Ganoderma water extract ethanol precipitation fine powder, standby; The cleanout fluid of supernatant and washing and precipitating, decompression recycling ethanol, the beta-schardinger dextrin-that adding 1-10 doubly measures makes dissolving under 70 ℃-90 ℃, in 30-50 ℃ of enclose, stirred 1-7 hour, and is dry below 40 ℃, is ground into fine powder, gets cyclodextrin clathrate; Get above-mentioned Ganoderma water extract ethanol precipitation fine powder, correctives and cyclodextrin clathrate that the mannitol that adding Ganoderma water extract ethanol precipitation fine powder 100%-200% doubly measures, the lactose that Ganoderma water extract ethanol precipitation fine powder 100%-200% doubly measures and Ganoderma water extract ethanol precipitation fine powder 10%-30% doubly measure, mix homogeneously, make granule, add at last and make the magnesium stearate that total amount 0.5-5% doubly measures behind the granule, mix homogeneously, compacting is in blocks, promptly.
Concrete preparation method is: get Ganoderma 3000mg, decoct with water 2 times, each 3 hours, collecting decoction filtered, filtrate is condensed into thick paste, add ethanol and make precipitation fully, precipitation reuse ethanol cleans 2 times, and precipitation is dry below 70 ℃, be ground into fine powder, get Ganoderma water extract ethanol precipitation fine powder, standby: the cleanout fluid of supernatant and washing and precipitating, decompression recycling ethanol, the beta-schardinger dextrin-that adds 5 times of amounts makes dissolving under 80 ℃, in 40 ℃ of enclose, stirred 4 hours, dry below 40 ℃, be ground into fine powder, get cyclodextrin clathrate; Get above-mentioned Ganoderma water extract ethanol precipitation fine powder, the mannitol, the lactose of 150% times of amount of Ganoderma water extract ethanol precipitation fine powder and the correctives and the cyclodextrin clathrate of 20% times of amount of Ganoderma water extract ethanol precipitation fine powder that add 150% times of amount of Ganoderma water extract ethanol precipitation fine powder, mix homogeneously, make granule, add the magnesium stearate make 1.5% times of amount of total amount behind the granule at last, mix homogeneously, compacting is in blocks, promptly.
The present invention is the ganoderma lucidum chewable tablet that is prepared from according to the method described above.
The present invention has changed and in the prior art extract has directly been added the method that adjuvant is made preparation, do not changing on the basis of extracting composition and curative effect, the triterpenes components of Ganoderma is separated from water soluble ingredient with the ethanol precipitation technology, and isolated triterpenes components wrapped up with cyclodextrin, make Ganodenic acid, Ganoderma lucidum (Leyss. Ex Fr.) Karst. acid, Ganoderma lucidum (Leyss. Ex Fr.) Karst. ketone, red spirit acid and cyclodextrin form the clathrate of molecular capsule, covered triterpenes components Ganodenic acid in the Ganoderma, Ganoderma lucidum (Leyss. Ex Fr.) Karst. acid, Ganoderma lucidum (Leyss. Ex Fr.) Karst. ketone, the bitterness of red spirit acid, use the ethanol precipitation thing of water soluble ingredient then, cyclodextrin clathrate adds the mixed accessories of mannitol and lactose, last compressed tablet; Solved because of have the problem of bitterness with the Ganoderma extract direct compression, also solved and be used alone adjuvant sticking takes place easily, the all undesirable problem of tablet molding effect, the easy moisture absorption of slice, thin piece after having avoided suppressing in flakes makes the chewable tablet of molding softening, the tablet quality wild effect, improved ganoderma lucidum chewable tablet quality stability, mouldability, preparation of the present invention is by chewing or containing, the abundant blood capillary in drug port transmucosal and Sublingual absorbs directly into blood, onset rapidly; Avoid first pass effect of hepar simultaneously, improved the utilization rate of medicine.
Therefore, compared with prior art, the present invention has steady quality, mouthfeel is good, absorption is fast, bioavailability height and the advantage of being convenient to take.
For confirming the seal effect of the present invention to cyclodextrin parcel Ganoderma triterpenoids constituents, the applicant adopts L
9(3
3) orthogonal experiment, studied the technology of beta-cyclodextrin inclusion compound volatile oil.By the investigation to clathrate yield and two indexs of bag branch rate, optimizing clathrate process is A
2B
3C
2, promptly with 5 times of beta-schardinger dextrin-amount enclose to volatile oil, the enclose temperature is 80 ℃, the enclose time is 4h, and verifies by the optimised process that experiment obtains.Test as follows:
Beta cyclodextrin wraps up the L that seals of volatile oil
9(3
3) orthogonal test:
1 material and instrument:
1.1 material: medical material meets " regulation under 2005 editions one each medicine item of Chinese pharmacopoeia through evaluation; Beta-schardinger dextrin-, Suzhou Gourmet Powder Factory produces, purity>98%; Reagent is analytical pure.
1.2 equipment: electronic thermostatic water-bath, Bei Jingjing are defended scientific instrument factory; SXJQ-1 type motor stirrer; The Scout11 electronic balance, Ao Haosi International Trading Company Ltd; 101-2 type drying baker, Shanghai City experimental apparatus head factory.
2 methods and result:
2.1 Ganoderma powder is broken into coarse grain, Ganoderma 3000mg decocts with water 2 times, and each 3 hours, collecting decoction filtered, and filtrate is condensed into thick paste, adds ethanol and makes precipitation fully, and precipitation reuse ethanol cleans twice, precipitates standby; The cleanout fluid of supernatant and washing and precipitating, decompression recycling ethanol, remaining material behind the collection recovery ethanol gets the Ganoderma triterpenoids constituents.
2.2 clathrate preparation technology's selection
Adopt the orthonormal design of experiments method, alternative condition sees Table 1.Adopt saturated water solution method-electronic stirring method to prepare clathrate, learn through test, mole ratio is bigger to inclusion rate, yield influence, so give the Ganoderma alcohol soluble substance: the beta-schardinger dextrin-mole ratio is added one group of level, so that more fully investigate.Adopt part additional method experiment arrangement scheme, experimental result sees Table 1.
The factor of table 1 orthogonal test and level
Level |
Ganoderma alcohol soluble substance: beta-schardinger dextrin-(mole ratio) |
Mixing time (h) |
Preparation temperature (℃) |
1 2 3 |
1∶3 1∶5 1∶7 |
2 4 6 |
40 60 80 |
With actual inclusion rate and yield is that index is carried out overall merit, the gained data as calculated, the best preparation process condition that draws Ganoderma alcohol soluble substance-Benexate Hydrochloride is as follows: the cyclodextrin that takes by weighing the molten thing of Ganoderma ferment, 6 times of amounts of Ganoderma alcohol soluble substance, place the heated and stirred still, add a certain amount of distilled water of people, aqueous solution is heated to 80 ℃, after stirring makes the cyclodextrin dissolving, put and be chilled to 40 ℃ and maintenance, stir 4h with motor stirrer with 2000rpm, cold drying is weighed and calculated yield, pulverized 60 mesh sieves then, be stored in the exsiccator standby.
With 3 batches of clathrates of method preparation of determining, its average yield is 85.75%, RSD=0.48.
The quality examination of 3 clathrates
3.1 microexamination
Beta-schardinger dextrin-is pressed this preparation method, preparation does not contain the blank clathrate of Ganoderma alcohol soluble substance, this product and pastille clathrate are respectively taken a morsel, place respectively under the biological microscope and observe: blank clathrate is the plate crystal of rule, and clathrate is the irregular block sprills.
3.2 taste test
With beta-schardinger dextrin-, Ganoderma alcohol soluble substance and the Ganoderma alcohol soluble substance-Benexate Hydrochloride packing of not marking respectively, there are 10 people (age 20-24 year) to participate in taste test, and the record result, clathrate does not have bitterness and slightly pleasantly sweet fully as a result.
3.3 the stability of clathrate
Get the Ganoderma alcohol soluble substance, Ganoderma alcohol soluble substance-Benexate Hydrochloride powder places 40 ℃ respectively, placed 3 months in the 75%RH climatic chamber, sampling and measuring was 1 time in every month, Ganoderma alcohol soluble substance and Ganoderma alcohol soluble substance-Benexate Hydrochloride downgrade that the result placed 3 months are respectively 2.06% and 1.26%, show that Ganoderma alcohol soluble substance-Benexate Hydrochloride is stable before the deadline.
4 discuss
4.1 optimization of preparation
Adopt orthogonal design that the preparation technology of clathrate is optimized, with the yield of clathrate, actual inclusion rate is index evaluation, the result shows that Ganoderma alcohol soluble substance-Benexate Hydrochloride is in host and guest's molecule ratio of 1: 5 mole ratio, and is better in 80 ℃ of electronic stirring 4h.
4.2 the coverage effect of clathrate
Studies show that, adopt the clathrate technology can cover bitterness fully, and beta-schardinger dextrin-also can improve stability behind enclose.And in mimic artificial saliva, the rate of release of clathrate is lower than the Ganoderma alcohol soluble substance, this may the Yin Lingzhi alcohol soluble substance be a medicine soluble in water, in 10min, just reach stable emission levels, and clathrate beta-schardinger dextrin-dissolubility boy Ganoderma alcohol soluble substance in water, clathrate needs can to discharge the Ganoderma alcohol soluble substance after the dissolving earlier in water, so slow releasing function is arranged, this has also solved the molten composition chewable tablet of ganoderol just or buccal tablet is rapid-action and deficiency that the persistent period is short.Therefore, can utilize the present invention, further make ganoderma lucidum chewable tablet or buccal tablet for clathrate foundation is provided with practical value.
In order to confirm that the present invention adopts the mixed accessories of mannitol and lactose to prepare mouthfeel, mouldability, the stability of ganoderma lucidum chewable tablet, the applicant has carried out related experiment, and its experimental result is as follows:
1, sample treatment
Medical material is handled and is got Ganoderma 3000mg, decocts with water 2 times, and each 3 hours, collecting decoction filtered, and filtrate is condensed into thick paste, and is standby.
Sample 1 is got above-mentioned thick paste, and is dry below 70 ℃, is ground into fine powder, adds the sucrose of 300% times of amount of fine powder, and mix homogeneously is granulated, and adds the magnesium stearate of making 1.5% times of amount of total amount behind the granule at last, mix homogeneously, compacting in flakes, promptly.
Sample 2 is got above-mentioned thick paste, and is dry below 70 ℃, is ground into fine powder, adds the mannitol of 300% times of amount of fine powder and the correctives of 20% times of amount of fine powder, mix homogeneously is granulated, and adds the magnesium stearate of making 1.5% times of amount of total amount behind the granule at last, mix homogeneously, compacting is in blocks, promptly.
Sample 3 is got above-mentioned thick paste, and is dry below 70 ℃, is ground into fine powder, adds the lactose of 300% times of amount of fine powder and the correctives of 20% times of amount of fine powder, mix homogeneously is granulated, and adds the magnesium stearate of making 1.5% times of amount of total amount behind the granule at last, mix homogeneously, compacting is in blocks, promptly.
Sample 4 is got above-mentioned thick paste, adds ethanol and makes precipitation complete, and precipitation reuse ethanol cleans 2 times, and precipitation is dry below 70 ℃, is ground into fine powder, gets Ganoderma water extract ethanol precipitation fine powder, and is standby; The cleanout fluid of supernatant and washing and precipitating, decompression recycling ethanol, the beta-schardinger dextrin-that adds 5 times of amounts makes dissolving under 80 ℃, in 40 ℃ of enclose, stirred 4 hours, and is dry below 40 ℃, is ground into fine powder, gets cyclodextrin clathrate; Get above-mentioned Ganoderma water extract ethanol precipitation fine powder, the mannitol, the lactose of 150% times of amount of Ganoderma water extract ethanol precipitation fine powder and the correctives of 20% times of amount of Ganoderma water extract ethanol precipitation fine powder that add 150% times of amount of Ganoderma water extract ethanol precipitation fine powder, mix homogeneously, granulate, add the magnesium stearate make 1.5% times of amount of total amount behind the granule at last, mix homogeneously, compacting is in blocks, promptly.
2, mouthfeel inspection
Adopt double-blind method with the medicine packing of not marking respectively the ganoderma lucidum chewable tablet of sample 1,2,3,4, preferred normal 10 people of the sense of taste (age 20-24 year) participate in taste test, and write down the result, the results are shown in Table 2.
Table 2
Sample |
1 |
2 |
3 |
4 |
Mouthfeel |
Earlier sweet back is bitter |
Inlet is promptly bitter |
Inlet is promptly bitter |
Dissolve all sweet |
As can be seen from Table 2, sample 1 is taken the relation of back owing to sucrose, and sensation is earlier sweet then bitter, and sample 2,3 is taken aftersensation bitter to the taste flavor, and sample 4 is not felt bitterness from taking the back up to dissolving, and pleasantly sweet; Sample 4 is exactly the chewable tablet according to method preparation of the present invention.
3, mouldability
Test tabletting, outward appearance, color and luster according to the pertinent regulations under 2005 editions one appendix ID tablet of the Chinese Pharmacopoeia item, the results are shown in Table 3.
Table 3
The sample mouth |
1 |
2 |
3 |
4 |
Tabletting |
Sticking very |
The sticking phenomenon is arranged |
Rare sticking phenomenon |
No sticking phenomenon |
Outward appearance |
Rough surface |
There is pit on the surface |
Smooth surface |
Smooth surface, light |
Color and luster |
Skin dark stain is arranged |
Evenly |
Evenly |
Evenly |
As can be seen from Table 3, sample 1 when tabletting because sticking, the time compression molding after slice, thin piece rough surface occurs and have some skin dark stains to exist; Sample 2 compares with sample 1, and the sticking situation take a favorable turn during tabletting, but still has sticking and surperficial pitted situation; Sample 3 samples 2 are that idol has sticking situation, smooth surface during tabletting relatively; There is not any sticking phenomenon during sample 4 tablettings, slice, thin piece smooth surface, light, color and luster is even, and sample 4 promptly is the chewable tablet according to method preparation of the present invention.
4, stability
Test sample 10g puts in the constant-temperature enclosed container, places 10 days under respectively at relative humidity 90 ± 5% conditions at 25 ℃, and in the 5th day and sampling in the 10th day, accurately test sample weight before and after the weighing test with investigation test sample moisture pick-up properties, the results are shown in Table 4.
Sample |
Before the test in the 0th day heavy (g) |
The 5th day |
The 10th day |
Test back heavy (g) |
Weightening finish (%) |
Test back heavy (g) |
Weightening finish (%) |
1 2 3 4 5 6 |
10.1672 10.1039 10.2016 10.0091 10.0112 10.0059 |
10.8084 10.7260 10.7388 10.0477 10.3010 10.1004 |
6.31 6.16 5.27 4.67 2.89 0.94 |
11.0444 10.9637 11.0106 10.5526 10.4697 10.1400 |
9.31 8.51 7.93 5.43 4.58 1.34 |
As can be seen from Table 4, sample 1,2 its weightening finish when the 5th day detects surpasses 5%, though sample 3 did not surpass 5% at the 5th day, near 5%, and at the 10th day also all above 5%; Have only sample 4 not surpass 5% its weightening finish in the 10th day.Show that thus sample 1,2,3 has higher hygroscopicity, sample 4 has stronger humidity-proof ability, can keep particulate drying property in a long time.
Data analysis from three experiments, the mouthfeel of sample 4, mouldability and humidity-proof ability all are better than other sample, and sample 4 is the chewable tablet according to the inventive method preparation, illustrate that the present invention can effectively solve the molding of the bitterness of Ganoderma, chewable tablet and the problem of high moisture absorption, the ganoderma lucidum chewable tablet of making can be guaranteed the stability of product quality.
After confirming that the present invention isolates three terpene components from Ganoderma extract, add precipitate again through beta-cyclodextrin inclusion compound and make the effectiveness of ganoderma lucidum chewable tablet on pharmacology, the applicant has carried out relevant pharmacological evaluation relatively, and its experimental result is as follows:
The research of this product ganoderma lucidum chewable tablet pharmacodynamic experiment:
1 materials and methods
1.1 sample
Ganoderma lucidum chewable tablet, the human body recommended intake is equivalent to Ganoderma 3000mg/ time usually.
1.2 experimental animal and cell
Kunming mouse, BALB/C mice, body weight 18-22g; Cavia porcellus, YAC-1 cell.Mice is divided into matched group (giving 0.5% starch fluid) and LINGZHI JIAONANG 58.5mg/kgbw/d, a Ganoderma tablet 58.5mg/kgbw/d and ganoderma lucidum chewable tablet 58.5mg/kgbw/d3 dosage form group at random.Ganoderma preparation is mixed with suspension with 0.5% starch fluid (boiling, cool off the back).The equal food grain feedstuff of all experimental animals, the drinking-water of freely ingesting.
1.3 test method
By the Ministry of Public Health health food function assessment assessment process and the method for inspection " in the immunoregulation effect method of inspection carry out.Give and tried the long-pending 0.4ml/20gbw of thing filling body of stomach, 1 time/d, continuous 28d.
1.3.1 dip prepares the inductive mouse spleen lymphocyte conversion test of ConA (mtt assay)
The filtering with microporous membrane degerming of RPMII640 culture fluid, add 10% calf serum, 1% glutamine (200mmol/L), penicillin (100U/ml), streptomycin (100 μ g/L) and 2 mercapto ethanol (5 μ mol/L), transfer pH to 7.0-7.2 with aseptic HCl (1mol/L) or aseptic NaOH (1mol/L), make complete culture solution; PBS buffer with pH 7.2-7.4 is made solvent, matching while using MTT solution (5mg/ml); Aseptic NaHCO with 3.5%
3Aseptic Hanks liquid is transferred pH to 7.2-7.4; Be mixed with the ConA liquid of 100 μ g/ml with distilled water, after the filtration sterilization ,-20 ℃ of preservations; In the 96ml isopropyl alcohol, add 4mlHCl (1mol/L) and make the acid isopropyl alcoholic solution.Each treated animal (female BALB/C mice, 10/group) continuous irrigation stomach to the 28d, every Mus cervical vertebra dislocation is put to death, and the aseptic spleen of getting is in the little plate that fills an amount of aseptic Hanks liquid, gently spleen is torn up with tweezers, make the individual cells suspension, 200 orders filter, and repeatedly wash, be suspended in the 2ml complete culture solution after centrifugal with Hanks liquid, with the blue dyeing numeration of platform phenol living cells, adjusting cell concentration is 2 * 10
6Individual/ml, divide two holes to put into culture hole, every hole 1ml, a hole adds 50 μ lConA liquid, and 5%CO is put in contrast in another hole
2, cultivate 72h for 37 ℃, to cultivate and finish preceding 4h, supernatant 0.7ml is inhaled in every hole gently, adds the RPMII640 culture fluid that 0.7ml does not contain calf serum, adds 5050 μ lMTT liquid simultaneously, continues to cultivate 4h.Cultivate and finish, every hole adds 1ml acid isopropyl alcohol, and the piping and druming mixing dissolves purple crystal fully, moves in the 1ml cuvette, and in 751 spectrophotometers, the 570nm place measures optical density.
1.3.2 the tardy paraphilia reaction of dinitrofluorobenzene (DNFB) inducing mouse (ear swelling method)
Take by weighing DNFB 50mg, put in the clean dried bottle, add the acetone Oleum Sesami solution for preparing in advance (acetone: 5ml Oleum Sesami=1: 1), adhesive plaster seals behind the close plug, mixing makes SNFB solution (face and use preceding preparation, take by bottle cap with 250 μ l syringes).During each treated animal (male mouse of kunming, 10/group) continuous irrigation stomach to the 23d, every Mus skin of abdomen loses hair or feathers with barium sulfide, the about 3cm of scope
2, evenly be applied in the auris dextra two sides with 50 μ l DNFB solution and attack, attack back 24h, mice is put to death in the cervical vertebra dislocation, and every Mus is weighed with the left and right auricle that card punch takes off diameter 8mm.
1.3.3 antibody-producting cell detects (Jeme improves slide method)
Get healthy sheep blood, after defiber, normal saline washing, be made into 2% (v/v) sheep red blood cell (SRBC) suspension with normal saline; Adopt 5 healthy guinea pig blood, separation of serum mixes, every 1ml hematocrit sheep red blood cell (SRBC) (defiber, repeatedly washing make) adds the 5ml guinea pig serum, put 4 ℃ of refrigerator 30min, often vibration, the centrifuging and taking supernatant is made complement (time spent dilutes by 1: 10 with barbitol buffer solution).During each treated animal (male mouse of kunming, 10/group) continuous irrigation stomach to the 23d, every Mus lumbar injection 2% (v/v) sheep red blood cell (SRBC) suspension 0.2ml.Behind the 5d (during do not stop to irritate stomach), the aseptic spleen of getting is put to death in every Mus cervical vertebra dislocation, in the little plate that fills an amount of aseptic Hanks liquid, the system single cell suspension, 200 orders filter, repeatedly wash the back in the 5mlRPMII640 culture fluid with Hanks liquid, counting cells, adjusting cell concentration is 5 * 10
6Individual/ml.After top layer culture medium (the 1g agarose adds distilled water to 100ml) heating for dissolving, 45 ℃ of water bath heat preservations, with equivalent pH 7.2-7.4, the Hank liquid of 2 times of concentration mixes, packing small test tube, every pipe are 0.5ml, add 10% sheep red blood cell (SRBC) suspension (v/v again, dispose with barbitol buffer solution) 50 μ l, 20 μ l splenocyte suspensions, mixing is poured on the slide of brushing the agarose thin layer rapidly, after treating that agar solidifies, the slide level is buckled and is placed on the horse, and in CO2 gas incubator incubation 1.5h, the complement with dilution is added in the groove of slide frame then, after continuing incubation 1.5h, counting hemolysis plaque number.
1.3.4 serum hemolysin is measured (blood clotting method)
Get healthy sheep blood, after defiber, normal saline washing, be made into 2%, 0.5% (v/v) sheep red blood cell (SRBC) suspension with normal saline.During each treated animal (male mouse of kunming, 10/group) continuous irrigation stomach to 23 d, every Mus lumbar injection 2% (v/v) sheep red blood cell (SRBC) suspension 0.2ml.Behind the 5d (during do not stop to irritate stomach), mice is extractd eyeball and gets blood in centrifuge tube, places 1h, 2000r/min, separate and collection serum, serum is used the normal saline doubling dilution in the Microhemagglutination bread board, every hole 100 μ l add 100 μ l 0.5% (v/v) sheep red blood cell (SRBC) suspensions again, mixing, the Microhemagglutination bread board is packed into and is added a cover in the moistening square position, hatches 3h in 37 ℃ of incubators, observes the hemagglutination degree.
1.3.5 mice carbon clearance test
India ink is made injection prepared Chinese ink for 4 times with the normal saline dilution; Take by weighing 1.0g Na
2CO
3Adding distil water is configured to Na to 1000ml
2CO
3Solution.Each treated animal (male mouse of kunming, 10/group) continuous irrigation stomach is during to 28d, and every caudal vein injects injection prepared Chinese ink, and ((0.1ml/10g.bw), timing immediately after the injection are got blood 20 μ l from the angular vein clump respectively in injecting back 2min, 10min, are added to 2ml Na
2CO
3In the solution, with 751 spectrophotometer meters at 600nm wavelength place's photometry density value, with Na
2CO
3Solution is as blank.Mice is put to death in the cervical vertebra dislocation, gets liver, spleen, blots the organ surface blood stains with filter paper, weighs.
1.3.6 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test (half intracorporal method)
Get healthy cock blood, after the washing of defiber normal saline, be made into 20% (v/v) chicken erythrocyte suspension with normal saline.Each treated animal (male mouse of kunming, 10/group) during continuous irrigation stomach to 28 d, every Mus lumbar injection 20% (v/v) chicken erythrocyte suspension 1ml is behind the 30min, mice is put to death in the cervical vertebra dislocation, face upward the position and be fixed on the Mus plate, abdominal skin is cut off in the center, and the abdominal cavity injects normal saline 2ml, rotate Mus plate 1min, sucking-off abdominal cavity washing liquid 1ml divides and drips on 2 microscope slides, is put in the enamel box that is lined with wet gauze, move to 37 ℃ of incubation 30min, take out, remove the not cell of paster, dry with the normal saline rinsing, fix with 1: 1 acetone methanol solution, 4% (v/v) Giems phosphate buffer dyeing 3min, the rinsing of reuse distilled water is dried, and light microscopic is observed down.
1.3.7 the NK cytoactive is measured [lactic acid dehydrogenase (LDH) algoscopy]
24h washes target cell (YAC-1 cell) cultivations of going down to posterity 3 times with Hanks liquid before using before the test, is 1 * 10 with RPMII640 culture fluid adjustment cell concentration
5Individual/ml.Each treated animal (male BALB/C mice, 10/group) continuous irrigation stomach to the 28d, every Mus cervical vertebra dislocation is put to death, the aseptic spleen of getting, in the little plate that fills an amount of aseptic Hanks liquid, the system single cell suspension, 200 orders filter, repeatedly wash the back in 2mlRPMII 640 culture fluid with Hanks liquid, counting cells, adjusting cell concentration is 5 * 10
5Individual/ml.Get target cell suspension, each 100 μ l of splenocyte suspension, add in U type 96 well culture plates, target cell nature release aperture adds each 100 μ l of culture fluid of target cell, and the maximum release aperture of target cell adds target cell and each 100 μ l of 1%NP40,5%CO is put in above-mentioned every three multiple holes of all establishing
2, cultivate 4h for 37 ℃, then with 96 well culture plates with the centrifugal 5min of 1500r/min, supernatant 100ul is drawn in every hole, reaction 3min, every hole adds the HCl 30 μ l of 1mol/L, at microplate reader 490nm place's photometry density value.
1.4 data statistics
With one factor analysis of variance in the SAS6.12 statistical software gained data between each experimental group are compared.
2 results and discussion
2.1 body weight, spleen/body weight ratio, thymus/body weight ratio
Ganoderma preparation the results are shown in Table 1-8 to the influence of mice body weight and weight gain, spleen/body weight ratio, thymus/body weight ratio.Its result shows: each dosage form mice body weight of Ganoderma preparation and weight gain, spleen/body weight ratio, thymus/body weight ratio and normal control compare, and difference does not have significance (P>0.05).
Table 1 splenocyte conversion test mice body weight and weightening finish
Dosage group (mg/kg) |
Number of animals (only) |
Body weight and weightening finish (g) |
Initial body weight |
14d |
28d |
Weight gain |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
20.0±1.0 20.0±0.8 20.0±0.9 20.0±0.8 |
23.5±0.5 23.8±0.8 23.9±0.8 23.9±1.0 |
26.8±1.3 26.4±1.0 25.8±1.3 26.3±1.2 |
6.8±1.7 6.5±1.2 5.8±1.7 6.4±1.7 |
Tardy paraphilia reflection test mice body weight of table 2 and weightening finish
Dosage group (mg/kg) |
Number of animals (only) |
Body weight and weightening finish (g) |
Initial body weight |
14d |
28d |
Weight gain |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
21.3±0.5 20.8±0.9 21.1±0.6 21.0±1.2 |
32.4±2.5 32.0±3.1 32.4±2.7 32.9±3.0 |
42.3±2.1 42.7±1.4 43.7±1.9 43.0±2.0 |
20.1±1.9 21.9±1.7 22.6±1.7 22.1±1.8 |
Table 3 hemolytic plaque test mice body weight and weightening finish
Dosage group (mg/kg) |
Number of animals (only) |
Body weight and weightening finish (g) |
Initial body weight |
14d |
28d |
Weight gain |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
21.3±0.6 20.9±1.1 21.2±0.8 21.3±0.9 |
32.8±3.0 32.7±2.7 33.4±2.7 33.2±2.1 |
42.2±1.9 42.8±2.3 42.2±2.6 43.8±3.1 |
20.9±2.3 21.9±2.6 21.0±2.1 22.6±3.0 |
Table 4 serum hemolysin test mice body weight and weightening finish
Dosage group (mg/kg) |
Number of animals (only) |
It is unclear heavily to reach weightening finish (g) |
Initial body weight |
14d |
28d |
Weight gain |
Normal control Ganoderma tablet LINGZHI JIAONANG Ganoderma chews the family |
10 10 10 10 |
?20.9±0.8 ?21.0±0.8 ?21.2±0.9 ?20.4±1.0 |
32.3±2.7 32.4±1.6 32.3±2.0 32.6±2.0 |
41.8±2.5 43.0±2.5 43.1±2.0 43.4±2.8 |
?20.9±2.4 ?22.0±2.7 ?22.0±2.6 ?23.0±3.0 |
Table 5 carbon clearance test mice body weight and weightening finish
Dosage group (mg/kg) |
Number of animals (only) |
Initial body weight |
Body weight and weightening finish (g) |
14d |
28d |
Weight gain |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
20.2±1.0 20.6±1.1 19.9±1.4 20.1±0.8 |
32.0±3.5 32.3±2.8 32.2±2.7 32.7±2.7 |
42.6±2.9 43.1±2.4 43.6±2.1 44.0±2.2 |
22.4±2.7 22.5±2.3 23.7±2.5 23.9±2.4 |
Table 6 peritoneal macrophage phagocytosis test mice body weight and weightening finish
Dosage group (mg/kg) |
Number of animals (only) |
Body weight and weightening finish (g) |
Initial body weight |
14d |
28d |
Weight gain |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
20.0±1.1 20.4±1.0 20.4±0.7 20.3±1.1 |
32.4±3.3 32.9±2.6 32.4±3.0 33.1±2.1 |
?41.7±4.0 ?43.6±2.5 ?43.7±2.4 ?43.8±2.4 |
?21.7±3.9 ?23.2±2.8 ?23.3±2.6 ?23.5±2.1 |
Table 7 NK cytoactive test mice body weight and weightening finish
Dosage group (mg/kg) |
Number of animals (only) |
Body weight and weightening finish (g) |
Initial body weight |
The 14th |
28d |
Weight gain |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
20.1±0.5 20.1±1.0 20.2±1.0 19.9±1.2 |
24.6±1.3 24.3±1.1 25.1±1.3 25.2±1.0 |
27.5±1.7 27.4±1.6 28.1±2.1 28.2±1.0 |
7.4±2.0 7.3±1.8 7.9±2.3 8.4±1.6 |
The variation (mg/kg) of table 8 mouse spleen body weight and thymus body weight ratio
The dosage group |
Number of animals (only) |
The spleen body weight |
The thymus body weight |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
2.68±0.24 2.65±0.31 2.60±0.33 2.62±0.15 |
1.57±0.23 1.70±0.24 1.85±0.24 1.64±0.32 |
2.2 the inductive mouse spleen lymphocyte conversion test of ConA (mtt assay)
ConA hole optical density-do not add ConA hole optical density difference to the results are shown in Table 9 is respectively organized in the inductive mouse spleen lymphocyte conversion test of ConA (mtt assay).
Table 9 Ganoderma preparation is to the influence of mouse lymphocyte multiplication capacity
Dosage group (mg/kg) |
Number of animals (only) |
ConA hole optical density-do not add ConA hole optical density difference |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
?0.010±0.007 ?0.012±0.006 ?0.017±0.012 ?0.022±0.013* |
* Dunnett ' s T check has significance with normal control group comparing difference, P (0.05)
ConA hole optical density in the his-and-hers watches 9-not adding ConA hole optical density difference carries out variance analysis, group difference significance (F=3.05, P<0.05).Through Dunnett ' s T check, Ganoderma preparation is respectively organized ConA hole optical density-do not add ConA hole optical density difference has significance (p<0.05) with normal contrast groups comparing difference.Illustrate when Ganoderma preparation is equivalent to human body recommendation absorption Ganoderma amount 3000mg to have the effect that strengthens the mouse lymphocyte multiplication capacity.
2.3 the tardy paraphilia reaction test of dinitrofluorobenzene (DNFB) inducing mouse (ear swelling method)
The tardy paraphilia reaction test of DNFB inducing mouse is respectively organized mouse right ear sheet-left auricle weight difference and be the results are shown in Table 10.
Auricle weight difference value is carried out variance analysis in the his-and-hers watches 10, and group difference has highly significant meaning (F=13.61, P<0.01).Through Dunnett ' s T check, each dosage form mouse right ear sheet of Ganoderma preparation-left auricle weight difference and normal control group comparing difference have significance (P<0.05).Illustrate when Ganoderma preparation is equivalent to human body recommendation absorption Ganoderma amount 3000mg to have the effect that the promotion mouse T lymphocyte is proliferated into primed lymphocyte.
Table 10 Ganoderma preparation is to the influence of the tardy paraphilia reaction of mice
Dosage group (mg/kg) |
Number of animals (only) |
Auricle weight difference (mg) |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
30.9±4.1 27.3±9.5 37.2±2.7 43.4±5.7* |
* Dunnett ' s T check has significance, P<0.05 with normal control group comparing difference
2.4 antibody-producting cell detects examination face (Jeme improves slide method)
This test is respectively organized the hemolysis plaque number and be the results are shown in Table 11.
Table 11 Ganoderma preparation is to the influence of mouse antibodies cellulation quantity
Dosage group (mg/kg) |
Number of animals (only) |
Hemolysis plaque number (individual/100 splenocytes) |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
252±28.2 268±27.8 296±51.0 382±58.1* |
* Dunnett ' s T check has significance, P<0.05 with normal control group comparing difference
Hemolysis plaque is counted the difference variance analysis in the his-and-hers watches 11, and group difference has highly significant meaning (F=17.79, P<0.01).Through Dunnett ' s T check, full powder 234 mg/kg dosage group hemolysis plaque numbers of Ganoderma and normal control group comparing difference have significance (P<0.05).Illustrate when Ganoderma preparation is equivalent to human body recommendation absorption Ganoderma amount 3000mg to have the effect that increases mouse antibodies cellulation quantity.
2.5 the determination test of serum hemolysin (blood clotting method)
The determination test of serum hemolysin is respectively organized mice serum hemolytic antibody product and be the results are shown in Table 12.
Table 12 Ganoderma preparation is to the influence of mice serum haemolysis rope antibody product
Dosage group (mg/kg) |
Number of animals (only) |
Antibody product # |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
1.685±0.240(48.4±1.7) 2.139±0.148(137.7±1.4) 2.203±0.132*(159.6±1.4) 2.300±0.123*(199.5±1.3) |
# antibody product is the logarithm average, is geometric mean in (); * Dunnett ' s T check has significance, P<0.05 with normal control group comparing difference.
The antibody product carries out variance analysis in the his-and-hers watches 12, and group difference has highly significant meaning (F=26.58, P<0.01).Through Dunnett ' s T check, three dosage form groups of Ganoderma preparation hemolysis plaque number and normal control group comparing difference have significance (P<0.05).Illustrate when Ganoderma preparation is equivalent to human body recommendation absorption Ganoderma amount 3000mg to have the effect that promotes that mouse antibodies generates.
2.6 mice carbon clearance test
The clearance test of mice carbon is respectively organized mice carbon and is cleaned up capability result and see Table 13.
Table 13 Ganoderma preparation is cleaned up the influence of ability to mice carbon
Dosage group (mg/kg) |
Number of animals (only) |
Phagocytic index |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
7.53±1.18 7.77±0.92 8.20±1.13 8.82±1.08* |
* Dunnett ' s T check has significance, P<0.05 with normal control group comparing difference.
Phagocytic index carries out variance analysis in the his-and-hers watches 13, and group difference has highly significant meaning (F=2.93, P<0.05).Through Dunnett ' s T check, each dosage form group phagocytic index of Ganoderma preparation and normal control group comparing difference have significance (P<0.05).Illustrate when Ganoderma preparation is equivalent to human body recommendation absorption Ganoderma amount 3000mg to have the effect that enhancing mice carbon is cleaned up ability.
2.7 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test (half intracorporal method)
Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test and is respectively organized Turnover of Mouse Peritoneal Macrophages and engulf the phagocytic rate and the phagocytic index of chicken red blood cell and the results are shown in Table 14.
Table 14 Ganoderma preparation is to the influence of Turnover of Mouse Peritoneal Macrophages phagocytic activity
Dosage group (mg/kg) |
Number of animals (only) |
Phagocytic rate |
Phagocytic index |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
18.6±4.1 21.4±4.1 27.6±6.0* 33.6±7.3* |
0.226±0.052 0.284±0.055 0.334±0.081* 0.416±0.101* |
* Dunnett ' s T check has significance, P<0.05 with normal control group comparing difference.
Phagocytic rate, phagocytic index carry out variance analysis in the his-and-hers watches 14, and group difference has highly significant meaning (F=14.45, P<0.01, F=11.64, P<0.01).Through Dunnett ' s T check, each dosage form group phagocytic rate of Ganoderma preparation, phagocytic index and normal control group comparing difference have significance (P<0.05).Illustrate when Ganoderma preparation is equivalent to human body recommendation absorption Ganoderma amount 3000mg to have the effect that strengthens the Turnover of Mouse Peritoneal Macrophages phagocytic activity.
2.8 NK cytoactive determination test (lactic acid dehydrogenase (LDH) algoscopy)
NK cytoactive determination test is respectively organized the NK cells in mice activity and be the results are shown in Table 15.
Table 15 Ganoderma preparation is to the active influence of NK cells in mice
Dosage group (mg/kg) |
Number of animals (only) |
NK cytoactive (%) |
Normal control Ganoderma tablet LINGZHI JIAONANG ganoderma lucidum chewable tablet |
10 10 10 10 |
9.1±5.3 34.3±16.0* 40.1±15.5* 47.9±15.0* |
* Dunnett ' s T check has significance, P<0.05 with normal control group comparing difference.
The NK cytoactive is carried out variance analysis in the his-and-hers watches 15, and group difference has highly significant meaning (F=15.00, P<0.01).Through Dunnett ' s T check, three dosage form groups of Ganoderma preparation NK cytoactive and normal control group comparing difference have significance (P<0.05).Illustrate when Ganoderma preparation is equivalent to human body recommendation absorption Ganoderma amount 3000mg to have the active effect of the NK cells in mice of promotion.
3 conclusions
Be equivalent to human body recommendation absorption Ganoderma amount 3000mg Ganoderma preparation 3.1 give the mice Ganoderma preparation, mice body weight and weight gain, spleen body weight ratio and thymus body weight ratio are not seen harmful effect through the filling stomach.
3.2 the inductive mouse spleen lymphocyte conversion test of ConA is the result show, the Ganoderma preparation that tries when being equivalent to human body and recommend taking in Ganoderma amount 3000mg, have the effect that strengthens the mouse lymphocyte multiplication capacity.
3.3 the tardy paraphilia reaction test of dinitrofluorobenzene inducing mouse is the result show, the Ganoderma preparation that tries when human body recommend to be taken in Ganoderma amount 3000mg, have and strengthen the effect that mouse T lymphocyte is proliferated into primed lymphocyte.
Show 3.4 antibody-producting cell detects result of the test, institute's Ganoderma preparation that try when human body is recommended to take in Ganoderma and is measured 3000mg, the effect with enhancing mouse antibodies cellulation quantity.
3.5 the serum hemolysin determination test is the result show, the Ganoderma preparation that tries when human body recommend to be taken in Ganoderma amount 3000mg, have the effect that promotes that mouse antibodies generates.
3.6 the clearance test of mice carbon is the result show, the Ganoderma preparation that tries when human body recommend to be taken in Ganoderma amount 3000mg, have and strengthen the effect that mice carbon is cleaned up ability.
Show 3.7 Turnover of Mouse Peritoneal Macrophages is engulfed the chicken red blood cell result of the test, the Ganoderma preparation that tries when human body recommend to be taken in Ganoderma amount 3000mg, have the effect that strengthens the Turnover of Mouse Peritoneal Macrophages phagocytic activity.
3.8 NK cytoactive determination test is the result show, the Ganoderma preparation that tries when human body recommend to be taken in Ganoderma amount 3000mg, have the active effect of the NK cells in mice of promotion.
In sum, ganoderma lucidum chewable tablet is the same with other Ganoderma preparation, has immunoregulation effect.