CN100356380C - Method of chromatogram fingerprint atlas, single medicine and preparation standardization - Google Patents

Abstract

The present invention provides a chromatographic fingerprint, the standardization of chemistry and curative effects, a program of a bar code of the fingerprint, enterprise resource planning (ERP) for common medicine and particularly traditional medicine and client relationship management (CRM), and a method for preparing an application database. The present invention comprises an instrument method on the basis of software and discloses a new method of a fingerprint and standardization so as to achieve the purposes. A traditional curative effect standardized method is associated with chemical characteristics of medicine and body fluid easily by means of the chromatographic fingerprint, and a reasonable foundation is provided for understanding a method for the purpose.

Description

The new method of a kind of chromatographic fingerprinting and single medicine and standard preparation

Technical field

The present invention relates to a kind of new method for chemistry and the chromatographic fingerprinting of criterion of therapeutical effect. Specifically, the present invention relates to be present in the chromatographic fingerprinting that has the organic and organic-metallic molecule of the visible radiation absorption characteristic of UV-in plant, animal or any other source that is used as single medicine or preparation medicine. The present invention is beneficial to one or more components that are present in the finger print image is carried out the encode of bar line. It also is beneficial to the commerce of the fingerprints database that contains relevant all information of medicine and uses, and exploitation and use ERP (Enterprise Resources Planning) and CRM (user's contact management) network working procedure resemble vendor etc.

The present invention has used the profile of the herbal medicine developed and preparation under code test (chemistry and the instrument) condition of suggestion and 3D chromatogram as the new method of medicine chromatographic fingerprinting.

In addition, the invention still further relates to the computer based method of analyzing these chromatograms. The authority that this new method especially is used as the chemical constituent of single medicine and preparation identifies.

Background technology

The history of conventional medicament in the world

After evolving many years, the men of old begins understanding oneself and attempts to understand the Nature. People begin to live under overcover, the cave and in the clan. The process of thinking encourages people to understand the Nature and the people's who lives internal action. People bring into use the available endemic plant of occurring in nature and animal to be used for people's demand every day, and wherein people use plant and animal to be used for people's diet and healthy needs.

In this process, people have sought and visited the in the world characteristic of various compositions, such as geography, celestial body and the medical characteristic of various biologies and plant. These begin to occur in the Stone Age in literature record. The life that people continue to find, standard and the available material of use nature are used for every day.

These different pieces in the world occur simultaneously in many places of the earth and in the place more flourishing as elementary civilization take knowledge larger development are arranged. So the history of medicine has direct relation with the history of civilization.

Conclude the existence of good medical system in India and can trace back to Harapa and Mohamzadaro (medical history of India, Dr Priya Vrit Sharma, Indian country research institute, New Delhi). In the civilization in India basin, medical system is very universal, wherein uses the medicine in vegetables, animal and mineral source. The Osadhisukta of the Rigveda is the most ancient file about plant and herbal medicine knowledge. Hindu medicine is given the credit to the traditional knowledge of Atharvaveda, and Ayurveda is called Upaveda. At the medical thesis " The Samhitas " of Charaka and Susruta, they are defined and describe a large amount of disease symptoms relations. This therapy also is to prescribe with systemic fashion and Rational basis aspect.

On the other hand, people recognize because individual difference aspect the composition of basis, thus biological phenomenon can not generally explain with the method for machinery, that is, when the prescription of begin to drink food or medicine to the patient, must keep Prakruthi firmly in mind. Great majority can be seen two metanotion such as Prakriti-Purusha, male-female, normal-unusual in all philosophy.

Disease is the performance of body fluid imbalance, and it must be to body and mind basis integrated treatment. So health be keep on the health, psychological and spiritual balance. Like this, health three dimension definitions that proposed by Susrtuta are optimal a kind of, and it is reflected in the definition of being adopted by contemporary WHO.

After browsing ancient literature, will find to come the standard medicine with the physical-chemical characteristic of drug substance. Use color, tissue, aroma and flavor as the assay method of curative effect of medication. Even understand the medicinal characteristic of medicine with the shape of medicine. Different philosophy used in treating and the summary of various factors in the table 1-6 that the specification rear section occurs, have been listed. The accompanying drawing 1 of specification has provided especially in the relevant separately philosophy of Ayurveda and Chinese medicine (Chinese Medicine of H.M.Sais and Daniel Reid, the Chinese herbal medicine handbook of Simon﹠Schuster) and other general philosophy and the details of concept. The curative effect of medicine finally depends on the chemical characteristic of component, and these components refer to can the chemical composition in mobiles to bring those of desirable variation.

Many Ayurvedic (a kind of Hindu medicine system) scholar is defined to medicine according to the color of medicine and curative effect and classifies. Listed short summary at specification than the table 7 that the rear section occurs. Like this, the physicochemical characteristics that should consider material and people is understood their characteristic and is used for realizing the therapy of required result for the treatment of. The table 8-9 that specification occurs has more afterwards provided about how to use physical characteristic (color) and chemical characteristic (taste) to understand the curative effect and they impacts on Human Physiology of medicine. When identical methodology has also been used in front portion work, and used instrument.

In general, being present in that component molecules in medicine and the food can slightly be divided three classes is polarity, middle polarity and nonpolar molecule. Total polarity of molecule depends on that the total parent's electricity that is connected on the molecule is connected with nucleophilic and the unsaturated molecule by the fit key connection. The human body that lives, animal body and plant also contain the molecule of same type, and wherein the molecule of opposed polarity plays different functions. With treating disease with the medicine of the identical polar of the chemical composition that causes disease, namely said as doctor Hanemann, the medicine that produces disease can be cured identical disease.

WHO is to the definition of herbal medicine:

WHO is defined as herbal medicine: " no matter what comprise that the ground of plant or under ground portion or other plant raw material or its composition make active component is the finished product that reset condition or galenical mark are crossed. Plant material comprises juice, glue, fat oil, volatile oil and any other natural material. Herbal medicine can also comprise excipient except active component. Contain plant material medicine can with the active substance combination of definition chemically, these active materials comprise the plant separated component of the chemistry definition that is not considered to herbal medicine. In addition, in some countries, herbal medicine also comprises the organic or inorganic active component that is not plant origin traditionally. "

So the purpose of these explanations is " in order to define the basic standard of estimating herbal medicine quality, safety and curative effect and to help thus the assessment that national legislation expert, scientific community and the producer relate to the proof data/submission of this series products/files ". In the rule of this assessment, traditional experience the refers to long-term use that should consider those products and medical science, historical and background nationality. The long-term definition of using can change according to country but should have decades at least. So evaluation should be considered to use the proof of knowledge material in the explanation of medical science/pharmacy literature or similar origin or the not free herbal medicine that limits. Also should consider the market mandate of like product. According to former report, carry out the evaluation of quality according to following parameters.

The explanation that WHO provides final products is for effect and active evaluation, needs to support the evidence of symptom and combination product. That many electicisms are comprised of well several active components and because the use experience of traditional remedies usually depends on combination product, should between old and new combination product, distinguish so estimate. Can cause incorrect evaluation to some conventional medicament to the consistent requirement of the evaluation of old with new composition. When tradition was used combination product, tradition used the proof data of (such as the classical works of Ayurveda, Chinese medicine, Unani and Siddha) and experience to can be used as the evidence of curative effect.

Except the proof data of the traditional knowledge of every kind of single component, should need the explanation of the new combination of knowing material is comprised effective dosage ranges and compatibility. Every kind of active component must all have contribution to curative effect of medication. Need clinical testing to prove the curative effect of new component and to the positive role of whole composition.

In this report, also mentioned and should describe production technology and prescription in detail, comprise the consumption of excipient. Should define like this finished product specification. Also should limit the method for discriminating and the quantitative approach of plant material in finished product if possible. If it is impossible differentiating active component, should be noted that the mixture (such as " chromatographic fingerprinting ") of differentiating a kind of property material or property material guarantees the steady quality of product. Finished product should meet the General Requirements of special form.

To the finished product of import, the country of origin should provide the affirmation standard of rule state. Should also provide the WHO certification scheme of the drug product quality of in international business, transporting. More detailed description to stability, security and in-service evaluation is arranged in the report of WHO.

Effective standard in the herbal medicine quality of international business exchange also need to be maintained close ties with between national research institution, and national research institution can examine according to standard all aspects of the use of product and herbal medicine. In order to manage or advocate the evaluation study to their curative effects, other drug used in their toxicity, security, acceptability, price and analog value and the modern medicine is compared.

So, as mentioned above, need a kind of authoritative method to quality control that proposes in this work. Clearly saying needs a kind of method that realizes above-mentioned purpose. The analysis that proposes will provide an answer to above-mentioned nearly all demand.

Existing standardized method:

Before explaining standardized method of the present invention, the below discusses existing standardized method (chemistry with curative effect) and chromatographic fingerprinting.

A. the prior art of chemical standardization

I) traditional:

Great Sage Charaka explains " the unilateral knowledge that can not come from it to the overall understanding of material " (Charaja Samhita VI.45) in his Charaka Samhita. This illustrates that clearly what do not consider all the components concerning the standardization of any medicine and curative effect all is invalid.

The quality of herbal medicine and quantity curve will change owing to many geography, ecological factor, acquisition time, the collection factors such as weather conditions local, when gathering age and collection.

The tradition medicinal herb grower gets used to being used for estimating color, the tissue of the chemistry of medicine and curative effect in their custom, gathering medicine when hearing and tasting according to effective special sense method.

These methods comprise original knowledge and understanding that the inside and outside therapeutic response of medicine and human body component is cured the disease. These knowledge change and depend on individual's technology and ability along with the difference of individuality. In fact, it will be very difficult using the method all to provide rational judgement to the explanation of any mechanism. So modern medicine is realized different purposes with instrument, eliminated like this individual factors also so that data and information are reproduced easily.

Ii) modern:

The treatment characteristic of any food or medicine will depend on its chemistry and physical state. Like this, understand the curative effect that chemical composition will help to understand medicine with their physical-chemical characteristic.

The physicochemical characteristics of medicine plays Main Function to the therapeutic activity of medicine. Use two kinds of parameter polarity and conjugacy just can study these characteristics of molecule. Polarity is owing to (nucleophilic) that be connected to the difference donations electronics on the molecule and connects nucleophobic (parent's electricity) group and the unsaturated double-bond of existence and electrochemical properties that triple bond bears results. They will affect the active or reactive speed of molecule in chemistry and biochemical reaction. Thereby will draw curative effect single or in groups molecule to the complete estimation of the total polarity of molecule makes them chemically have activity with acology. So any standardization of estimating above-mentioned characteristic all will be for the activity of understanding them.

According to the polarity that relates to most molecule electricity-chemical characteristic, the physical arrangement of molecule is also at the reactive important role of molecule. The number that is connected to the active site on the molecule is more, and their reactivity will be stronger. The molecule of conjugation more (having other two keys and triple bond), it chemically and the activity aspect the therapeutic will be stronger.

The second parameter that affects molecular activity is that the solid of the atom of structure generation difference is arranged. Owing to this reason, isomers (how much and chirality) molecule plays an important role in biologically active. This three-dimensional selectivity characteristic is so that molecule has the high selectivity activity in human body, because the large number of biological passage is arranged less than interacting and intrusively concurrent working in the human body. So it is extremely important that the chemistry of chiral drug has become. Say that with other terms key (chiral molecules) can not opened different lock (acceptor).

Usually plant has prepared in a large number/comprehensive library of molecules, and these molecules have identical parent structure and connect functional group difference on it. For example, the flavonoids in the naturally occurring flavonoids, aurones class and chalcones can work as multi-medicament with the single plant with this quasi-molecule.

Usually the molecule that has undersaturated and multiple conjugation can absorb the electromagnetic radiation of UV-visual ray (200-800nm). But when compound and ray reaction, they can absorb special wavelength (absorption maximum) according to their chemistry, conjugation and architectural characteristic. It is known as characteristic wavelength. Molecule enough has more than one absorption maximum according to their 26S Proteasome Structure and Function performance. When compound absorbed particular color from the integral body of white light, it can show other, and not have absorbed color be last color. Like this, material can absorb shades of colour and show different colors from white light according to their chemical composition, and the color that shows is owing to connect the cause of its various functional groups. (table 10 that the back of specification occurs has been explained same cause). The method of the chemistry of molecule and physical characteristic is to have adopted same principle in chromatograph.

For example, red medicine absorbs in the scope of 500-600nm. Like this, all red medicines all have the peak in the wave-length coverage that this has special construction and activity. So, the treatment of the color useful as drug of medicine and the method for chemical property. In ancient times, medicine is to carry out classification on the acology according to color. It is identical that present method confirms. Describe color and show that from Figure of description Fig. 2 of body fluid relation different colors is to the effect of various disease.

In the Figure of description 3, the finger-print of two kinds of Shilaji samples making with the inventive method has shown the difference of the chemical curve of two kinds of samples. Shilaji is the carbonaceous material that is formed for many years at subsurface storage by vegetables and animal. Majority is because volcanic rock floats to and damaged flora and fauna on the forest. Become carbonaceous material through many geographical change afterwards and be called Shilajit. Can obtain galore in Russia. It is used as medicine in the maximum magnitude in the world. Find similar general molecular formula in the Shilajit sample of separate sources although people observe, the kind of also finding the conjugate property of molecule is different. This is so that this medicine is different on curative properties, so the finger-print of this type is useful.

In the modern chemical analysis method, carry out the mensuration of active component percentage composition by various analytical instrument, these active components comprise alkaloid, flavones, enzyme, vitamin, volatile oil, fat, carbohydrate, protein, ash, are insoluble to ash and the pristine fibre of acid. Explained how to carry out standardization with modern science in the example below.

It is reported that (.html such as WWW//Shilajit, fulvic acid) Shilajit is one of used very important medicine in the Hindu medicine system, reporting has many compounds with fulvic acid in Shilajit, and is called active component. Collect the material that contains pitch and make medicine from the soil that stores for many years, can see that to be stored in the soil time more of a specified duration, therapeutic activity is better. But on the whole earth, difference geographical from whole samples of collecting in different regions in the world can not produce identical molecule. Other factors that affect the chemistry of these medicines are purge processes, and this also needs standardization.

It is reported that (WWW//Herbology.html) most standardization are that single main component is carried out, these main components are proved to be the most helpful to human body with science on experience. The common like this standardization of carrying out is carried out the activated specific molecular of existing discovery tool. But the synergy that should consider to be present in other compounds in the medicine concerning its effect produces the general curve of medicine.

It is reported that the ethanol extract of (the tendril .html that WWW//puncture vine is barbed) Tribulus terrestris L has anti-urinary calculus activity. In addition, this extract has also demonstrated significant diuretic activity. Now reported from alkaloid Harman in the herbal medicine with from the banisterine in the seed. This plant has comprised saponin, and hydrolysis can obtain steroid sapogenines. It is reported that what find in many molecules of in nature flavonoids to have active component is saponin. Heavy metal and total saponin content (20%w/w) analysis have been provided analysis report.

It is reported that (WWW//Charak_com.-quality control page or leaf-html) human life is the comprehensive of consciousness, body ﹠ mind factor, these India's philosophy with Ayurveda are relevant, think that wherein Pitta, Kapha and Vata are the bases of people's general health. In the conventional method explanation of criterion of therapeutical effect, provided more detailed standardized method.

It is reported (WWW//standard Cao Yaotiquwu herbal medicine scholar's observation, Dr.Micheal Tierra L.Ac.O.M.D.html) because the law of European compulsory licensing, it forces the herbal medicine standardization gradually. The standardized implication of herbal medicine is defined as active component or indicates the quantitative of extract, and they are the most contributive to activity.

Someone is interpreted as (Frank R Stermitz etc., the PAINS/2 month 15, the 4th page of 1433-1437 of 2000/ the 97th volume) antibacterial activity of extract is that jamaicin works to antibiotic, and does not have it not all right owing to there is 5-hydroxyl hydnocarpin in the plant dyer's barberry. So, when processing herbal medicine, be not only a kind of active component, should consider the synergy of whole components.

(Dr Ranjit Roy Choudary, herbal medicine be to the mankind's health, and Searo20) what is clearly mentioned is that standardization and which country should carry out standardization for people use medicine better to WHO in the regional public publication of WHO.

Understand the effect of acidity and basicity with the technique of the extraction solvent extraction drug component of different pH values by careful understanding. This will help to understand medicine discharges medicine in people's enteron aisle of different enteron aisle pH mechanism. The effect of research acidity and basicity and in understanding the medicine curative effect, carefully understand the effect of acidity and basicity. Thoroughly the acidity of the organic and inorganic molecule of research and basicity are understood the characteristic shown in the table 11 that specification occurs later. Acidity and basicity have shown the effect (hold healthy, Devendra Vora, Navaneet publishes (India) company) of acidity and basicity in health.

It is reported (WWW//chewing gum .html) in a research, observe the people with acidic system and absorb more pollution than the people who sets up suitable blood basicity. Acid/alkali (pH) balance is important to normal cell function. More details in this article, have been provided. So the research that acidity and basicity (organic or inorganic) term is called " polarity " will provide the information of curative effect of medication. So the current method that can do this work will be used for understanding the curative effect of medicine more. Use the method for advising, can set up acidity and basicity for the criterion of therapeutical effect of medicine.

Above-mentioned list of references will have been explained conventional standardized method with being reported, wherein separate single component and quantitatively and qualitatively relatively will be present in the same compound of studying the sample medicine with preparation property scale.

(pharmaceutical grade sabal in a report, the US patent 6 of Khwaja etc., 039,950), reported the biologically active of different single cuts of studying the ethanol extract of sabal by suppress to measure in the determination method IC50 in androgen body fluid receptors bind. The TFA of total extract and single cut has been discussed to be measured. Identify the cut that contains ethyl linoleate and ethyl laurate. Each cut is all calculated its activity, compare to measure them to the inhibition of androgen body fluid receptors bind with total biologically active of sample. Discriminating molecular weight and single fat acid content also participates in the bioactive calculating. Compare with total percentage activity of linoleic acid and ethyl laurate cut, calculate total biologically active of this extract.

In the standardization of conventional medicament, should consider master curve to the curative effect of herbal medicine. So, in current computer based instrumental method, the gross activity of all components is regarded as suggestion in the whole traditional concept world. Advise the finger-print of medicine as processing medicine especially vision aid and the evidence of the multiple purpose of conventional medicament. Before method of the present invention was discussed, the below provided existing analytical method.

The existing analytical method of chemical standardization

Improve and use Modern Methods and instrument more outstanding on the method for quality control of medicine undoubtedly. As explained above, the improvement on analytical method has caused more accurate herbal medicine results and has helped the preparation standard extract.

Although have the conventional method of discriminating medicinal plant such as the method for organoleptic, microscopical and physics, neither one can draw believable discriminating in the middle of their, and when providing the finger-print of vegetable material, but relates to as much as possible chemical profile.

So the someone advises that chromatographic fingerprinting is very useful to the quality control of medicinal plant, can replace other organoleptic and microscopical research. Because finally be that chemical constituent participates in the curative effect of medicine and other characteristics of herbal medicine in a large number; The analysis data of chemical constituent can provide believable curative effect of medication. This resembles single finger-print and provides discriminating to it.

Up to the present, thin layer chromatography (TLC), high performance thin layer chromatography chromatography (HPTLC) and high pressure (efficient) liquid phase are the methods that is commonly used to analyze organic or organic metabolic compounds and finger-print. But the analytical method of more various common existence in the subordinate list 12 that specification occurs later provides the general comment of their pluses and minuses.

Specification appended Fig. 4 has demonstrated how " chromatographic fingerprinting " of the chromatogram that obtains from the TLC method as mark on commodity. Except the mensuration of component, finger-print does not provide more information.

After observing table, it is found that can analysis of compounds the optimal technology of mixture be " chromatogram ", after with suitable analyzer Isolation and Identification, provide the curve of mixture.

Outside available dissimilar chromatographic technique, optimal be " high pressure liquid phase " (HPLC). Although at the current thin layer chromatography that used, the development that brings at HPLC instrument and splitter is so that revolution has occured in the chromatography analysis field.

The most drug analysis is with the form of the chromatogram at peak report, because most of in official method and pharmacopeia what report is the molecule of mobile phase wash-out. These components are to analyze with suitable analysis analyzer behind wash-out on the HPLC splitter.

Usually chromatography uses normative reference (inner or outside) to carry out. Do not have marker, chromatographic peak just can not provide the chemical characteristic of the compound of any institute wash-out, so such analysis is nonsensical. Therefore component quantitatively and qualitative (spectrum or chemistry) be confirmed to be unclear.

In the qualitative and quantitative analysis of medicinal material/medicinal material (single or patent medicine), mainly spectrum and the chemical characteristic to the composition that elutes provides emphasis after analyzing samples. This analysis is according at analyte the impact (being called the UV-visible radiation) of electromagnetic radiation and they being carried out its reaction. In existing chromatography method, analysis report is that chromatogram is any chemical characteristic such as polarity and the UV-visible absorbance characteristic that can not provide component. Chromatogram can not demonstrate the existence that does not absorb or have at other wavelength (be called 225 or 245nm) molecule of different " absorption maximum " at this wavelength. If sample is 100% purity, if it is a known molecular, then the analysis at fixed wave length is acceptable, but under the drug condition of the active component that has more than one, this is very unpractical. In Fig. 5-12, provided at some shown examples of single wavelength, wherein provided the chromatogram at different wave length. The chromatogram that neither one is single can provide the relevant complete information that is present in the chemical characteristic of the component in the medicine, and the active component that especially exists in conventional medicament is not only a kind of. But when comparison colours spectrogram and finger-print, the use of finger-print can be understood.

So any chromatogram that exists in the special Wave strong point is the whole chemical profile that existing composition in single medicine and the medicament can not be provided. Like this, in its report chromatogram just part and can not be accepted. Any analytical method that can not provide whole analytical informations all is can not be received on science.

In the herbal medicine that existence has a dissimilar molecule of different spectral characteristics (absorption maximum) is analyzed, will be nonsensical analysis report or chromatogram at the chromatogram of fixed wave length.

In the use of herbal medicine, medicine is to do as a wholely to come for some the standard care conditions described in ancient literature and the manuscript. Thus, the concept of seeking active component is incomplete, and this is because what the medicinal characteristic of medicine was worked is total profile. Like this, any analytical method that can not show the whole chemical characteristics that are present in all components in the drugs will be useless.

Because many ecological factors such as acquisition time, the maturity of collecting place, collection and the monsoon condition in collecting link etc., the quantitative and qualitative analysis figure of herbal medicine also is different.

Frank R Stermirtz etc. has mentioned because there are not existing other component main components of extraction species explained earlier can not play its function, so the collaborative of other components that exists with main component also is no less important.

B. the standardized prior art of acology

I) conventional method:

Great Hindu medicine sage has understood and has defined the concept of Hindu medicine by limiting biological characteristic, component and body fluid. They also know the internal and external relation between them. In nearly all Traditional Philosophy, basic concept all comprises characteristic and to the effect of people's personality. It is said that human body is to be grouped into (Saptadhatus) by seven types one-tenth. General disease (Tridosha) is three types. The substance characteristics of any material causes (Pancha bhutas) by five kinds of elements in the universe. The effect of the different permutation and combination of these elements will be unhealthful. So, will help physics and chemistry characteristic of understanding them etc. to the understanding of these characteristics, and their curative effect. Different local philosophers have also been developed these concepts to be fit to their traditional and social in the world.

In ancient times (in the Samhitic of India early stage and Susrutic early stage), doctor use Nadisastra (feel the pulse) when diagnosis understand Tridosha (Vata, Kapha and Pitta) thus state know patient's health status. The arteries and veins (not being the pulse of heart) of research specific type is explained the type (Priya Vrat doctor Sharma of the Indian country academy of sciences " Hindu medicine history ") of patient's principal disease.

It can be used for understanding the main doshas type of patient and to each invalid doshas for the treatment of disease in when diagnosis. But, this art of feeling the pulse be limited in some through tools of many training able, individual skill with people ability in. Like this, not that each traditional doctor can both put into practice it.

In order to overcome this problem, now developed with standard the physical-chemical performance of medicine and the field of people's personality understanding. With the internal and external relation of these characteristics of standard and unhealthful character, doctors have been developed pharmacy and medicine-therapeutic field so after deliberation.

The curative effect of medicine be define with the purposes that bring the material of (drug effect) effect can for human body (Kriyagunavat) and be the cause of the consolidation function of many factors (samavayikaranam), work the formation as the many lines one of dress.

In a word, biology has two kinds of main Types, plant and animal. It is said that also namely soil, water, gas, fire and space (said according to the Panchabhutas in the Ayurveda) form by five kinds of large elements in the world. The key property of these materials is two types, is exactly strong (strong) and weak (soft). If we are according to this senior coherent logic, we can say that all effects all are the causes of the different permutation and combination series of above-mentioned performance, like this in their variation that has drawn qualitatively large-scale performance and material.

In the philosophy in most traditional medicines world, the creator of this system has considered the unification of the character of five kinds of compositions. They will help to understand patient's disease or disorder. In Ayurveda, this unification is called Purusha, in the traditional Chinese medical science, is called Yin and Yang. Provide traditional Chinese medical science system in the table 6 that specification occurs later and how to have used above-mentioned two kinds of factors, how their classification and definition have been come standard treatment and disease.

The traditional Chinese medical science is to classify to human body with glad negative and positive according to expression is sad as mentioned above. These factors work to medicine and biological various performances. Be used for carrying out keeping of these factors by what consider chemical factor, physiologic factor and social factor in history. In the time of most of, Chinese medicine has direct or indirect relation with the various bio-energies center that is arranged in human body. Identical principle has been used in the acupuncture field. Other factors and the traditional Chinese medical science reported in other philosophy have similitude. After the panchabhautic concept, the concept of Tridosha (Pitta, Kapha and Vata) plays a major role in Hindu medicine and what consist of human body is seven kinds of compositions (Saptadhatus). Ayurveda is believed historical Life Philosophy and is emphasized that prevent disease is greater than the treatment disease.

The historical approach of Ayurveda advocates that soul, mind and the human body are three kinds of indispensable parts of life, when function equilibrium and universal time coordinated, this state is called health (Arogya). When their imbalances and imbalance, this state is called disease (Vaishamya).

According to Ayurveda, the physiological property of various systems when Tridosha has kept the function equilibrium state. In other words, the coordination of Tridoshas has formed health, and imbalance causes disease. So, be to process Tridoshas at the most of time of any disease for the treatment of. For the disease in should locating is selected medicine.

Disease is defined as " bringing sad and painful anything (Purusha) to the people is exactly disease ". Disease can be divided into Four types: 1. unexpected (agantavaha); 2. physical injury (Sarirah); 3. mind injures (Swabhavikah) of (Manasah) and 4. natures. Because this reason, most traditional concepts except with put into practice with standardized life method also locate inner spiritual human body factor and treat disease.

Generally these diseases are divided into three classes: 1. recoverable (Sadhya) 2. relievable or controllable (Yapya) and 3. (Asadhya) that can not cure. As mentioned above, majority think those human body diseases with their reasons be by conflicting thridoshas by Vata, Kapha and Pitta and blood are separately or mutually combine and cause. But, be not that the disease that is caused by above-mentioned reason is to treat with diverse ways such as mental illness. Here it is why traditional concept be used for saying that institute's spiritedness human body factor considers to treat the cause of disease. The single characteristic of doshas as explained below.

Must, being summarised as Vata or vayu dosha and processing endocrine, nervimuscular and neural activity, main or total function that all these can both cause life causes that the food of gas formation can be classified at its taxology. Pitta dosha refers to digestion and chemical functional or common rasa kriya, and Kapha dosha comprises these factors that form, stability and conjugacy and lubricity factor are provided. With regard to first dosha, " Vata " is considered to affect other two factors, and it all is principal element to any disease. Provided the accurate description of these factors at the other place of this paper.

The reduction of Vata causes the whole blunt of activity. So reducing this blunt medicine will be Vata Hara. The reduction of digestic property is called Pitta dosha. To be Pitta Hara on the pharmaceutical properties of increase digestic property or activation enteron aisle mechanism. The minimizing of liquid or mucous membrane will cause hot-tempered, internal injury slightly, empty stomach, arthrochalasis, thirsty, weak and continuous insomnia. These are cardinal symptoms of kapha imbalance. Damaging on any pharmaceutical properties of these imbalances will all be Kapha Hara.

In order to understand the more common different traditional medicines world, various philosophy have all been provided the detailed description of all factors. Table 1 and table 2 (specification occurs later) have provided the accurate description of India Ayurvedic philosophy and various compositions.

So in order to understand the curative effect of medicine or food, people need to know their physics and chemistry performance. In ancient times, people with the method that affects organ as taste, the color of news and material comes for understanding these characteristics. Fundamental characteristics is divided into: 1. taste (Rasa), 2. quality (Guna), 3. strength (Virya), the 4. effect (Vipaka) of the state after the assimilation and component and 5. special roles (prabhava, how much with isomer molecule optics).

It is that Dohas (disease), Dhatus (component) and Malas (secretion) are mainly used to process and treat disease or imbalance that three kinds of factors are arranged. If the above-mentioned characteristic of medicine is consistent with dosha, it will be compromised or comprehensively, disease just is treated like this.

Change to the different of another situation with a kind of situation of difference along with patient doshic advantage with the classification that comes the source rule medicine according to the Ayurvedic pharmacodynamics. In other words, between dravya gunas (ydwu texq) and dosha (disorder), be associated. In order to be had different single doshas or the patient treatment same disease of its combination, it is essential increasing or reduce one or more medicines. So the drug therapy of Ayurvedic is more individualized according to patient's dosha advantage and the vague generalization when not resembling the drug therapy of modern medicine. It is unique and more reliable in the drug therapy of Ayurvedic to identify the characteristic compatible with doshas (Rasa, Guna, Veerya, Vipaka and Prabhava) and be.

Ii) modernism of criterion of therapeutical effect:

Above-mentioned concept is not considered in existing drug therapy. Pharmaceutical Chemist only to from plant, separate, purifying active component and the structure explanation is interested and they give the pharmacologist with these active components and study their biologically active. The pharmacologist then screening have pharmacologically active molecule, set up the mechanism of action and with modern medicine in used existing standard drug to the effect of relatively estimating it.

Concept must not help the traditional medicine doctor like this, because the separation of active component has changed the animal economy feature of medicine and their curative effect dramatically.

What replacement obtained the analyses such as solvent extractable matter cut, active component from single plant is people's cell solvent from medicine whole analysis of extracting compatible with cell membrane in use and the body, and this is used for majority the pharmacologically active of this class medicine of evaluation.

In the modern clinic test that realizes criterion of therapeutical effect, carry out (being four during international the use) according to three phases, comprise a large amount of crowds. The information relevant with the novel drugs of acceptance to the medicine effector generally comprises:

1. chemical constitution

2. pharmacology rank

3. preparation details

4. animal data comprises the data of toxicity research

5. the clinical pharmacology data comprise pharmacokinetics (reaction of medicine in human body)

6. pharmacodynamics (medicine effect in vivo)

In the world other countries to special research and the situation of this medicine

8. biology-equivalent data.

Thereby the research major concern of phase I be the security of estimating medicine know medicine be how absorption of human body, metabolism with secretion, it also is used for assessing side effect and dosage.

The research of second stage is to adopt random device to be used for specially testing curative effect. One group of patient gives real medicine and second group of use placebo.

In the research of phase III, carry out a large amount of tests and come the validity, benefit of drugs and the scope of possible side reaction. After being successfully completed this step, industrially will make pharmaceutical market.

In the late period that III and IV stage are studied, will there be several purposes in drugmaker. Research will help to understand the effect that new drug is compared with existing medicine. To know the long-term efficacy of new drug and on the impact of patients ' life quality. To know the cost efficiency with respect to other tradition and new treatment drug therapy.

But all above-mentioned researchs are expensive and consuming time. They all do not consider patient's ecological factor, heredity rule (as what implement), psychology, society and effects other variable elements in India family and marital relations. This incites somebody to action so that efficacy of drugs is confined among the crowd of particular group or hereditary form.

C. the prior art used of bar line code and Enterprise Resources Planning (ERP) user contact management

Preparing the proprietorial modernism of any commodity is bar line code. Concerning all commodity transactions, bar line code is widely used in many ways. In order to ensure the uniformity of medicine and ownership commodity, the present invention advises using the new method of bar line code.

It is reported that (Peernet bar line code store (the dynamic X servlet of Jvav e-business)) can provide 1800 features and 2700 data (even 9,99,999 numbers) to produce the bar code of any bar to the bar code software that is purchased. When value data and/or umerical number input bar line code software, it can be by the bar line code pattern special for the user produces of the specific logical in the software ownership.

When readable line code vending machine seen bar line code on (by electronic eyes or photoreceptor) commodity, the bar line code that generates like this can occur and be presented at have all products/subsidiary " display window " of mark detail file information in. Can be from any ERP and CRM read this line code using in the world by network.

Be used at present the classified catalogue of coding medicine and Related product, these catalogues specifically do not comprise any chemical characteristic of the product of mentioning in this method.

Main purpose of the present invention provides a kind of chromatographic fingerprinting, chemistry and criterion of therapeutical effect and bar line code from the new method as the organic and organic-metallic molecule of plant, animal or natural or the manmade materials of medicine.

Another object of the present invention provides a kind of herbal medicine of defects and new chromatographic fingerprinting of preparation got rid of.

Another purpose of the present invention provide a kind of use software newly developed according to research and represent that according to the medicine traditional concept conjugacy of curative effect is to being present in the complete chemical analysis of the composition in the medicine.

Another object of the present invention provides the new method of herbal medicine chromatographic fingerprinting, and it is used for differentiating fast and is present in real image and these composition curative effects of using the compound in the medicine.

A further object of the invention is to use herbal medicine and the profile of preparation and the new chromatographic fingerprinting that the 3-D chromatogram provides herbal medicine and preparation thereof.

Another purpose of the present invention provides a kind of new method of herbal medicine chromatographic fingerprinting of the doping that is used for checking the compound that exists in the used medicine.

Another purpose of research is that the preparation standard analytical parameters is as using same solvent alcohol extract, 0-60 minute identical running time, identical mobile phase acetonitrile and pH at the phosphate buffer solution and identical UV-visible range 200-800nm of 5.5-7.5 scope at present.

Another purpose of the present invention be from 3-D and outline-color spectrogram according to the classification of polarity and conjugate pair drug ingedient and quantitatively and estimate medicine to its will work curative effect of body fluid of (infringement).

Another object of the present invention provides the bar line code at the Molecular Selection peak that provides in the image.

Another purpose of the present invention is the database for the preparation of the bar line code of the finger-print of developing of all types of databases uses.

At present another purpose of research is the display window that has the sample particulars for the sample production of all finger-prints, and these particulars comprise 3-D and profile finger-print, bar line code, source particulars (factory or country), date of manufacture, the term of validity, the dosha that reports, used single component, their mensuration, lot number, sale number, M.R.P. (the highest retail price) etc.

Another purpose of the present invention is that display window is connected with separately bar line code, easy processes and displays window in all are used, and no matter when they are used as in data and the information source.

Another object of the present invention is the database of the display window produced like this of preparation and it is connected with each bar line code, is used to Enterprise Resources Planning (ERP) and user's contact management (CRM) in the business transaction net of all medicines and sample.

A further object of the invention is the database of preparation bar line code, display window and any information, is used in particular for flowing of administrative department's control medicine both at home and abroad.

Another object of the present invention is that the UV-visible spectrum of compound will provide the conjugacy of the molecule except molecular polarity and the concentration of the single concentrate of molecule.

To be that the basis of differentiating chemical composition defines scope of the present invention thereby other purposes of the present invention are uses of the finger-print of profile and 3-D chromatogram.

Another object of the present invention is that exploitation is a kind ofly given doping food and drug sample, substituted and the method for the commercial sample finger-print of conflict food and drug sample and food and drug sample and be used for differentiating purity and doping level.

Thereby another object of the present invention is the method for the finger-print of organic in a kind of any type sample of exploitation and organic-metallic composition reaches quality control and process standardization with chemical composition in the discriminating sample various purposes.

Another object of the present invention is for quality control and criterion of therapeutical effect and develop the method for Allopathic, Ayurvedic, Homoeo, Siddha, Unani, Chinese medicine, Tibetan medicine and Kampo (Japan) drug sample finger-print.

Another object of the present invention is for chemical composition change in the sample of studying naturally occurring or synthetic preparation and differentiates the chemical composition in them and the fingerprint spectrum method that its standardization is developed.

Another object of the present invention is for chemical composition change in the sample of studying naturally occurring or synthetic preparation and differentiates the variation of their chemical compositions that cause owing to geographical, ecology, genotype and phenotype changing factor and a kind of fingerprint spectrum method that its standardization is developed.

Another object of the present invention is to differentiate the chemical composition in them and a kind of fingerprint spectrum method of developing for the chemical composition of studying single herbal products and patent medicine sample and for chemistry and criterion of therapeutical effect.

Another object of the present invention be for the variation of chemical composition in postgraduate's matter sample and discriminating and standardization wherein chemical composition and a kind of fingerprint spectrum method of developing.

Another object of the present invention is the preparation large database concept, and this database will provide the general rule of many concrete group of plant curative effects, and these plants are divided into one group according to disease specific or curative effect.

Another purpose of the present invention provides a kind of method, the method can with the conjugacy of single component and whole medicine and polarity understand with the physicochemical property of standardization medicine such as traditional treatment standard in used color.

Another purpose of the present invention provides a kind of method, the method can with the conjugacy of single component and whole medicine and polarity understand with the physicochemical property of standardization medicine such as traditional treatment standard in used taste (Rasa) is namely sour, salty, hot, bitter and puckery (is called respectively Amla in Ayurveda, Lavana, Katu, Tikta, Kashaya).

Another purpose of the present invention provides a kind of method, the method can understand with the physicochemical property of standardization medicine such as quality, effect, absorption after metabolin or the chirality (in Ayurveda, being referred to as respectively Guna, Veerya Vipaka and Prabhava) of the improvement of this class and property such as molecule.

Another object of the present invention provides a kind of method, this method can with the conjugacy of single component and whole medicine and polarity understand with the physicochemical property of standardization medicine such as traditional treatment standard method in used weight, gently, hot and cold, soft, sliding, gentle, dried, slow, violent (in Ayurveda, be referred to as respectively Guru, Laghu, Sheeta, Ushna, Snigdha, Manada, Teekshna).

The present invention relates to a kind of use chromatographic fingerprinting technology mensuration and discriminating from plant or animal extracts, the method for the composition in nature or synthetic source, the step that the method comprises has:

I) extract organic or organic-metallic molecule with appropriate solvent;

Ii) with the high pressure liquid technology resulting extract in the step (i) is carried out compartment analysis;

Iii) produce profile and the 3D chromatogram of the composition that elutes according to pH and polarity;

Iv) resulting 3-D and outline-color spectrogram are changed into colored image, analyze each color of colored image with the coordinate system of all 3-dimension characteristics of this image of indication by using embedded software;

V) demonstrate concentration along with the various components of time wash-out;

Vi) produce chromatogram on the basis of color analysis, this figure has the peak relevant with molecular characterization in each retention time;

Vii) the UV-visible absorbance characteristic by various components in the image identifies compound in this component;

Viii) according to polarity and conjugacy to differentiating, measure and classification according to polarity, middle polarity and the less or nonpolarity compound that elutes;

Ix) use X-axis do reservation time, Y-axis make wavelength, R prepares bar line code as the blue pixel number to the peak of selecting as green pixel number, B as red pixel number, G;

X) produce finger-print with bar line code database and differentiate different compounds in the sample.

So the new basis of the method is in the new method of the chemical composition spectrum property shown in 3-D and the outline-color spectrogram as finger-print. The chromatogram that the method generates provides conjugacy and the polarity of single molecule in the medicine that provides curative effect of medication.

In a molecule, its UV-visible absorbance ability depends on molecular structures. When having one of two keys or triple bond in molecule or the structure, but it is called conjugation. Molecule more can conjugation, and it just more has chemistry and physical activity. So molecule more can conjugation, its therapeutic activity is just stronger. Like this, measure the curative effect that conjugacy will provide medicine. So using conjugacy is new meaning of the present invention to criterion of therapeutical effect.

Suggestion is used for new method the quality control of herbal medicine and preparation, most finger-prints that are used for conventional medicament and standardization (chemistry with treatment) do not resemble for only analyzing active component (active component is unknown at many herbal medicine) and namely analyze medicine at single wavelength. The physics and chemistry performance (be exactly UV-visible absorbent properties and polarity) of the method except providing compound is total give the profile of the chemical composition that exists in the conventional medicament. In the first of the method, with the finger-print image of generating medicine. But because this image can not become the analysis data, so developed the quantitative and qualitative analysis data that a kind of computer based method provides composition in the chromatography report form. This also is the new meaning of the inventive method equally.

The reactivity of any molecule will depend on two keys and the number of triple bond and parent's electricity and the nucleophilic position on the molecule that is present in the molecule. Provide the position of electronics and reception electronics to produce difference in the molecule total electrical charge. This is just so that molecule has polarity. So the polarity of molecule can provide relevant this molecule to provide or receive the ability of electronics to other molecules. This has just determined the activity of molecule. Like this, the information of molecular polarity will illustrate the reactivity of molecule. In the method, the chromatogram that provides of the method can provide at the fingerprint place conjugacy and the polarity that is present in the composition in the medicine. Like this, thus the method can be used for the curative effect that the standardization of medicine is understood medicine with conjugacy and the polarity of medicine. This also is the new meaning of the method for advising.

As mentioned above, the UV-visible spectrum of compound and polarity will show conjugacy and the polarity of compound, have so just shown chemistry, the pharmaceutical characteristic of medicine. The collection of illustrative plates profile of all the components will become the blueprint that is present in the composition in herbal medicine and the preparation as now described " finger-print " in a figure. This just becomes than existing will express the better herbal medicine discriminating of UV-visible light method and standardized method such as the peak. The conjugacy of component and polarity except component quantitatively do not resemble at the resulting conventional chromatogram figure of single wavelength.

As described in the traditional standardized method, can understand and their curative effect of standardization with the color of medicine. The color of molecule is interpreted as absorbent properties to UV-visible range ray. The absorbability of special wavelength depends on structure, functional group, conjugation and degree of unsaturation. The conjugacy of molecule is stronger, and the wavelength of absorption will be longer. So the UV-visible absorption of any molecule is widely used in the quantitative and qualitative analysis of component. Color and the curative effect of various medicines have been provided in ancient times in the document.

The final color of molecule is because the cause of the special chemical of molecule. But study when identical, also can understand chemical property. In ancient times, the quality control of the color of flame for metal and products thereof, this has just comprised basic chromatographic principles. Like this, will be useful to the curative effect of specializes in chemistry character and medicine to research and the understanding of electromagnetic radiation. In present finger-print and standardized spectrophotometer method, used identical principle. The topmost novelty of the inventive method comprises uses instrument " with retention time (polarity) finger-print to be divided into different treatment regions according to wave-length coverage (conjugation) and to understand the curative effect of single or patent medicine (conventional term) " for main program with software.

Use the computer based software of developing, bar line code is made at the Molecular Selection peak that provides in the image. Wherein X is retention time, Y is wavelength in the outline-color spectrogram and the absorption value in the 3-D chromatogram, R is red and the component of expression Cmax, G be green and expression than the component of small concentration and B is blue and also represent component than small concentration, the coordinate that these have current software to provide, this software is to input any commercially available again salable line code software, and they can join the bar line code that generates one-component or various ingredients in this software. The image of finger-print can be seen in coupled display window. It all will show when the electronic eyes of no matter when selling machine read this line code. This product to factory or country has been guaranteed the ownership of image (finger-print) and bar line code. This also is another new meaning of the inventive method.

When the polarity of having fixed post and mobile phase polarity when changing according to the order that increases or reduce always, on reversed-phase column, the component that is present in the sample will be at first to be high polar compound, then to be that the component of middle polarity is the sequentially eluting of nonpolarity component subsequently. In elution fraction, note carrying out according to the order that increases or reduce polarity, do not obtained total eluent by wash-out thereby the component of so any polarity will can not be retained on the post. Identical with reversed-phase column at the post Semi-polarity of normal phase and the order of wash-out with performance, but reversed in order. In the post of normal phase, according to the polarity order of the used mobile phase of wash-out, nonpolarity component is wash-out at first, then is polar compound.

What therefore, obtain has a treatment standard that the finger-print that increases or reduce tactic chemical constituent according to polarity can help to bring relevant medicine. This is another new meaning of institute's recommend method.

The image of the outline-color spectrogram that obtains after analyzing is divided into three districts at X and Y-axis. Conjugacy (absorption of special wavelength ray) is placed on the Y-axis polarity is placed on the X-axis, resemble the wash-out of controlling component with the polarity of mobile phase composition. Now just as reported in the literature, Y-axis is to indicate scale according to the curative effect that is as the criterion with wavelength (color). Whole image is divided into 6 chambers, and wherein chemical constituent has special conjugacy and polarity. This successively with the chamber in the curative effect of component proportional. Like this when the medicine finger-print, the color that special wavelength is absorbed take expression and have specific polarity as standard is calculated in total color in this district and to the curative effect of existing component and is made an explanation. So, use the method to realize historical criterion of therapeutical effect and chemical standardization.

The wash-out of most of samples carries out to low polarity mobile phase from high polarity mobile phase. In this finger-print, the component that (district-1) exists in the first district will be high polarity like this. Identical mode is used for other districts, in middle polarity district (district-2) wash-out be the middle polarity component and in nonpolarity district (distinguishing-3) wash-out be low or nonpolarity component. This mode is just in time opposite when using the normal phase post, and this is because aforesaid elution property.

Most high polar molecules chemically have higher level of reactivity, so also have higher biological. When they enter the first of digestive system mouth, they will will begin in a minute and act on biosystem and enzyme and exist wherein. Then this component will enter stomach and intestines, and here they will carry out different variation (effect after the absorption, the Vipaka in Ayurveda), and this is because digestive juice and their enzyme are present in this position. In absorption process, high activity (high polarity) molecule just demonstrates their curative properties when will begin in a minute with biological systems responses. It is compared, in Ayurvaeda, the enteron aisle of human body partly is divided into the Pitta district, here high polar molecule plays a major role. Heat production mechanism will play an important role and relate to biomechanism in disease. This shows that indirectly the high response molecule is exactly high polar molecule. After the absorption, will be transported to heart to them and relate to its part with the blood of the composition of all absorptions. Then blood will be sent to the different parts of human body. In Ayurveda, the part that goes up of human body is defined as the Kapha district, cold mechanism will play an important role here. So the molecule of middle polarity will play an important role in relating to the mechanism in this district.

Low polarity and non-polar component only can be transported by blood and be entered human body, only have like this mechanism that obtains chemical constituent by blood in human organ, so human organ will become the last catalogue of polarity. The mechanism of non-polar oil, fat and other this quasi-molecules and human body is divided into the material that Vata lacks of proper care and all these class imbalances all are the use same types treats.

Low polarity and non-polar component will be eluted in the area postrema of finger-print. Therefore, this district (district-3) thought the Vata district. Like this, the key property of molecule can differentiate that according to their polarity understanding easily it will work to what disease (dosha). So this method can be used for the criterion of therapeutical effect of medicine.

The form that is present in like this whole components in district-1Pitta district, district-2Kapha district, the district-3Vata district and all is with pie chart occurs, and it has represented the ratio of efficacy of drugs to every kind of disease. Like this, the medicine of component that contains 50: 20: 30 orders will be the tridoshahara medicine of 50%: 20%: 30% order. Like this, the curative effect quantitative criterion. By add other drug make medicament carry out any one or two kinds other the increase of doshas or minimizing and preparation the required appropriate formulation of the special entity for the treatment of.

Like this, has the information that conjugation, absorption and the 3-D chromatogram of the fingerprint of polarity scale will provide relevant curative effect of medication. Use all three-dimensional character analyses of this figure will give the 3-D chromatogram of medicine quantitative. If for example the 3-D chromatogram is regarded as " the band cover cap " that mates from the whole cap of 3 dimensions and is had the quantitative and qualitative analysis characteristic different with other samples that the degree that it mates will appear in the qualitative and quantitative analysis report. Here the cover of cap is likened to the molecule peak of special wavelength. The cap that contains most molecules will resemble the cap of many covers. The extremely simple and clear method of contrast and analysis will be provided with the coupling of three-dimensional coordinate like this. The coordinate that it matches will provide qualitative and degree that it matches will provide the quantitative data of study sample. Can make it become possibility by the special software for this purpose preparation. This will become the final method of quality control. This is another new meaning of the inventive method.

The present invention also relates to take software as main composition 3-D chromatogram and the data processor of color profile diagram, this processor contains calculation element and can be:

A. one kind can be take the shades of colour (having issue explanation, lifetime, the cleanup standard mentioned) selected as the coloured profile diagram of standard analysis analyzer (color of extraction), these colors represent the in time concentration of the various components of wash-out and the polarity that is as the criterion with retention time;

B. the analyzer of the 3-D chromatogram of all 3 dimension specificity analysis medicines that can use image;

C. one kind can generate and has with the polarity particular order device of the chromatogram at the peak of the various retention times that are associated of the molecular characterization of wash-out in time;

D. the UV-visible absorbance characteristic of various components is differentiated compound discriminator in these molecules in the enough images of energy;

E. one kind according to the polarity of molecule and conjugacy by finger-print being dividing in treatment region on X and the Y-axis with the biology that is present in the various components in the drugs reported, the therapeutic activity device of mapping mutually;

F. enough X-axis of advising that software provides of energy are done the reservation time, Y-axis is made wavelength, R produce the bar line code at selected peak as the coordinate system of blue pixel number as green pixel number, B as red pixel number, G device;

G. one kind can generate the finger-print of sample and the database of bar line code, the device that various databases are used in Enterprise Resources Planning (ERP) and customer relation planning (CRM) program easily; And

H. one kind can all generate display window to used sample in all Enterprise Resources Plannings (ERP) and customer relationship planning (CRM) type commercial programs " the device of database.

Used abbreviation in the patent document

1.ERP: Enterprise Resources Planning

2.CRM: user's contact management

3.UV-Visible: at the electron radiation of 200nm-800nm scope

4. organic molecule: in structure, contain C, H, N, O, the molecule of S basic element

5. organic-metallic molecule: in structure, contain metal and C, H, N, O, the molecule of S basic element

6. outline-color spectrogram: arrange analyzer with the data shown chromatogram type of 200nm to the electromagnetic radiation scanning samples generation of 800nm by photodiode. The chromatogram that produces like this will provide retention time at the X-axle, provides absorption region (nm) at the Y-axle. To use different colors to represent the variable concentrations of one-component.

7.3-D chromatogram: it also is to produce with the equipment of above-mentioned same type. It will be the UV-Vis chromatogram that more information is provided for each component after separating from mixture. Use this spectrogram to help to differentiate component.

8. Ayurveda: India's philosophy of the explanation medical science of being shown by the India sage and the compilation science of healthy rule.

9.Oshadisukta: in Rigveda, provide the chapter as the medicinal characteristic details of medicine.

10.Rasa, Guna, Vipaka and Prabhva: in the Hindu medicine system, be used for understanding the medicine of drug effect and the different physicochemical properties of material

11.Lokapurusha Samanya: natural equilibrium rule

12.Tri dosha: in the Hindu medicine system, pass through Pitta, three kinds of body fluid of Kapha and Vata researching human body

13.Prakriti-Purusha: in the Hindu medicine system first than be a mother (woman) and second for people (man)

14.Pitta: a used term in the Hindu medicine system, a kind of body fluid or be used for that is used for representing the human body of individual character in the finger mark degree medical system represent a kind of disease of digestion and chemical functional or rasa kriya in the human body

15.Kapha: a used term in the Hindu medicine system, a kind of body fluid or be used for that is used for representing the human body of individual character in the finger mark degree medical system represent to provide in the human body a kind of disease of formation, stable and combination and lubricated factor

16.Vata: a used term in the Hindu medicine system, be used in the finger mark degree medical system representing individual character human body a kind of body fluid or be used for representing a kind of disease of neurologic and endocrinological in the human body and neural activity

17. geographic factor: relate to the regional disparity of soil function and the soil moisture of soil composition

18. ecological factor: in the torrid areas, the areal variation of monsoon condition and temperature

19. the method to stimulus to the sense organ: end user's sensory medicinal property is such as 1. tastes (resembling acid (Amla), salty (Lavana), hot (Katu), bitter (Tikata), puckery (Kashaya)) 2. color, the 3. discrimination method of smell and 4. quality etc.

20. the visible character (color, size) of taste (Rasa physicochemical property) and feel (quality) and all physical propertys and resemble taste be present in medicine in the relevant pharmaceutical characteristic of the chemistry of one-component

21. the state with the performance of medicine such as taste (Rasa), quality (Guna), effect (Virya), after absorbing and effect and the special role (Prabhava) of component (Vipaka) make pharmaceutical standards

22.Saptadhatus: in the Hindu medicine system, be present in 7 kinds of elements such as Rasa (body fluid), Rakta (blood), Mamsa (muscle), majja (marrow), Asthi (skeletal system), Medas (fat) and Shukra (reproducibility) composition in the human body.

23.Panchbhutas: existing 5 kinds of native elements such as Prithivi (soil), Ap (water), Teja (fire), Vayu (gas) and Akasha (space) in the used world in the Hindu medicine system

24.Nadisastra: the used pulse that passes through the research people is explained the science of the health status of human body in the Hindu medicine system

25. in the Hindu medicine system, the factor that causes disease is interpreted as Agantavaha (unexpectedly), Sarirah (human body produces), Manasah (mind produces) and Swabhavikan (nature)

26. according to the Hindu medicine system disease is divided three classes, their be recoverable (Sadhya), relievable or controllable (Yapya), incurable (Asadhya)

27. conjugacy: if molecule contains singly-bound or two key and provides and accept characteristic electron, just be referred to as conjugacy. In the visible collection of illustrative plates of the UV-of molecule, can see. The energy that sigma and Pi electron institute absorb in the molecule that activates according to electromagnetic radiation, molecule will absorb the ray of special wavelength. The absorption maximum of molecule will represent the conjugacy of the molecule of studying like this.

28. polarity: if in the electrochemical properties of molecule, have difference, be referred to as polarity. This depends on the atom that is connected on the molecule with power supply (nucleophilic) or electronics acceptance (parent's electricity) group or function base, and molecule has difference with the electric charge in molecular orbit. This is so that molecule has positive terminal and negative pole end. The molecule of this type is referred to as polar molecule. The degree of electric charge and type will determine that the character of molecule is polarity, middle polarity and nonpolarity.

29.HPLC gradient or triplex system: can change moisture or the ratio of aqueous solvent not with the HPLC instrument of two or three liquid pumps. This will help to control as requested total polarity of mobile phase.

Used some abbreviations in the software:

1.JDK:Java exploitation box

2.Con: the outline-color spectrogram

3.3-D:3-dimension chromatogram

4.WOS: there is not scale

5.X: the retention time of expression chromatogram

6.Y: the absorption value in the expression 3-D chromatogram and the wavelength in the outline-color spectrogram

7.R: at the locational red density of special pixel

8.G: in the locational green density of special pixel

9.B: in the locational density of special pixel

Summary of the invention

One embodiment of the invention relate to a kind of chromatographic fingerprinting, chemistry and criterion of therapeutical effect and from as the plant of medicine, animal or natural available or manmade materials organic and organic-new method of metallic molecule bar line encode.

Another one embodiment of the present invention relates to the new method of the chromatographic fingerprinting of a kind of herbal medicine of having got rid of defects and preparation.

Another embodiment of the present invention relates to a kind of method, and it uses the complete chemical analysis of software to being present in the composition in the drugs and representing the conjugacy of curative effect according to the medicine traditional concept of developing.

Another embodiment of the invention relates to a kind of new method of herbal medicine chromatographic fingerprinting, and it is used for differentiating fast and is present in the real image that uses the compound in the medicine and these composition curative effects.

A still further embodiment of the present invention relates to the new ideas of the chromatographic fingerprinting of the herbal medicine of a kind of profile of using herbal medicine and preparation thereof and 3-D chromatogram and preparation thereof. They are to arrange analyzer (PDA) at the photodiode of high efficiency liquid phase to produce. This has described the spectroscopic data that is present in the component in the herbal medicine under the analysis of experiments condition.

Another embodiment of the present invention relates to a kind of method that is used for any natural drug extract of the molecule of the electromagnetic radiation that contains the ray (200-800) that can absorb UV and visible range or any scope is carried out chromatographic fingerprinting.

In another embodiment of the present invention, the UV-visible spectrum of compound provides the conjugacy of molecule and the concentration of different molecular.

In another embodiment of the invention, the finger-print that the same medicine that extracts under different pH condition generates is to help to understand the release of medicine in the intestinal tract of everyone different pH values.

In another embodiment of the present invention, in single image " chromatographic fingerprinting ", shown the UV-visible spectrum of all components.

In another embodiment of the present invention, finger-print becomes blueprint with existing component in herbal medicine or the preparation, and it can be used for measuring and the quick medicine of studying of differentiating.

In another embodiment of the present invention, the finger-print that uses profile and 3-D chromatogram is the basis of differentiating already present chemical composition and/or the new chemical composition that forms.

In another embodiment, the UV-visible spectrum of compound and polarity have represented conjugacy and the polarity of compound, and have represented thus the chemistry/pharmaceutical characteristic of medicine. This in single figure the curve of spectrum of all components make as present " finger-print " recommended and be present in that component becomes blue fingerprint image in herbal medicine and the preparation. This becomes than existing more outstanding herbal medicine differentiates and standardized method, because these peaks have represented the visible performance of the UV-of component or conjugacy and polarity, does not resemble conventional chromatogram figure with the qualitative component of single wavelength.

In another embodiment of the invention, realized " according to the scale of wavelength (conjugation) and retention time (polarity) finger-print is divided into different area for treatment and understands the curative effect of single or patent medicine (traditional) " by using program based on instrument and software.

In another embodiment of the invention, from the mass data storehouse of using the method preparation, provided the many general rules of curative effect of the concrete group of plant, the group of classifying according to acology is used for disease specific.

In another embodiment of the invention, use X, Y, R, G, B use bar line code software to produce bar line code, like this so that the pharmaceutical properties industrialization as the coordinate of selecting the peak on the finger-print.

In another embodiment of the invention, use all three-dimensional characters of medicine collection of illustrative plates to analyze its 3-D chromatogram. If regard as the 3-D chromatogram from " the band cover cap " of the whole caps coupling of 3 dimensions and have the quantitative and qualitative analysis characteristic different with other samples, then its degree of mating will appear in the qualitative and quantitative analysis report. Here the cover of cap is likened to the molecule peak of special wavelength. The cap that contains most molecules will resemble the cap of many covers. The extremely simple and clear method of contrast and analysis will be provided with the coupling of three-dimensional coordinate like this. The coordinate that it matches will provide qualitative and degree that it matches will provide the quantitative data of study sample. Can make it become possibility by the concrete software for this purpose preparation. This will become the final method of quality control.

In another embodiment of the present invention, related to a kind of profile of herbal medicine and preparation and method that the 3-D chromatogram provides the new chromatographic fingerprinting of herbal medicine and preparation used. They are to arrange analyzer (PDA) at the photodiode of high pressure liquid phase to produce. It has described the spectroscopic data that is present in the herbal medicine and has the component of single-minded rank polarity under the analysis of experiments condition.

Related to use compound UV-visible spectrum in another embodiment of the invention, it provides the conjugated nature of molecule and the concentration of opposed polarity molecule.

In another embodiment of the invention, related to a kind of method that single image " chromatographic fingerprinting " shows the UV-visible light spectrogram of all components that is provided at. This finger-print makes thus the component that is present in single medicine or the preparation become blue spectrogram and is used for measuring and differentiating fast the medicine of research.

In another embodiment of the invention, relate to profile and 3-D chromatogram and limit scope of the present invention as the basis of differentiating chemical composition.

Related to a kind of method with standard analysis parameter in another embodiment of the invention, standard analysis parameter wherein is as using same solvent alcohol extract, 0-60 minute identical running time, identical mobile phase acetonitrile and pH at the phosphate buffer solution and identical UV-visible range 200-800nm of 5.5-7.5 scope.

In another embodiment of the invention, thereby the method Application standard analytical parameters is determined the treatment general rule such as the sample finger-print that makes concrete treatment group with same solvent alcohol extract all samples.

The method that in another embodiment of the invention, has related to a kind of method to doping food, medicine and chemical example finger-print and discriminating purity and doping level.

The method that in another embodiment of the invention, has related to a kind of method to substitute food product, medicine and chemical example finger-print and discriminating purity and substitution degree.

The method that in another embodiment of the invention, has related to a kind of method to controversial food, medicine and chemical example finger-print and discriminating purity and substitution degree.

In another embodiment of the invention, relate to a kind of method of the commercial sample finger-print to food and medicine and differentiate the method for purity and substitution degree.

Relate to a kind of method that organic in any type sample and organic-metallic ingredients fingerprint is existed chemical composition wherein with discriminating for the various purposes of quality control and process standardization in another embodiment of the invention.

In another embodiment of the invention, relate to a kind of with Allopathic, Ayurvedic, Homoeo, Siddha, Unani, Chinese medicine, Tibetan medicine and Kampo (Japan) drug products finger-print so that quality control and chemistry and the method for criterion of therapeutical effect.

Another embodiment of the present invention relates to a kind of research in natural with fingerprint spectrum method and has the variation of chemical composition in the sample and the chemical composition in these samples is identified and standardization.

Another embodiment of the present invention relate to a kind of with the natural variation that has a chemical composition in the sample of fingerprint spectrum method research and to since in geology and the caused sample of ecological factor the variation of chemical composition identify and standardization.

Another embodiment of the present invention relate to a kind of with fingerprint spectrum method study chemical composition in the naturally occurring sample variation and to since in heredity and the caused sample of phenotype changing factor the variation of chemical composition identify and standardization.

Another embodiment of the present invention relates to a kind ofly to be identified with standardization to carry out application chemistry or acology standardization with the variation of various chemical compositions in the sample of the synthetic preparation of fingerprint spectrum method research and to chemical composition in the sample.

Another embodiment of the present invention relates in a kind of herbal products of drug sample of or preparation single with fingerprint spectrum method research various chemical compositions and the chemical composition in the sample is identified to carry out chemistry or acology standardization.

Another embodiment of the present invention relates to a kind ofly to be identified and standardization with the variation of chemical composition in the fingerprint spectrum method postgraduate matter sample and to the chemical composition in these samples.

Another embodiment of the present invention relates to a kind ofly to be studied the variation of chemical composition in the food of the single of different trade marks or preparation and the drug products and the chemical composition in these products is identified to carry out chemistry or acology standardization with fingerprint spectrum method.

Another embodiment of the present invention relates to a kind of method for preparing database, and this database can provide many general rules to the curative effect according to concrete group of plant of disease specific or treatment classification.

Another embodiment of the present invention relates to a kind ofly to be set up finger-print and according to polarity and conjugacy that 3-D and outline-color spectrogram reflect the component of medicine is classified and quantitative method.

Another embodiment of the present invention relates to following a kind of method, the method provides the bar line code at the selected molecule peak that is provided by image, wherein contain software computer (microchip, the Doyle chip switch, the hardware and software of encrypting) provide coordinate, they are respectively X-retention time, Y-wavelength, R-red pixel number, G-green pixel number and B-blue pixel number; Sell again in the bar line code software if these data are input to any commercialization that contains the software of advising, then can produce bar line code. Some embodiment of these images have shown the coordinate at this distinctive specific peak of product: X-retention time, Y-wavelength, R-red pixel number, G-green pixel number and B-blue pixel number have also comprised the bar line code of making therefrom simultaneously.

Another embodiment of the present invention relates to a kind of database for preparing the bar line code of the finger-print of setting up, and this database is applicable to all types of database applications.

Another embodiment of the present invention relates to a kind of method of display window of finger-print of all samples for preparing generation. In this " display window ", shown the out of Memory on analysis, lot number (batch number, lot number), M.R.P and the label of various compositions, these compositions of all details of sample such as 3-D and profile finger-print, bar line code, the details (factory or country) about the source, date of manufacture, expiration date, the dosha that reports, use. If the bar line code on this label of automatic vending machine demonstration, then it can demonstrate its additional display window. This helps to understand chemistry and the treatment reliability of (sold/purchased) medicine of selling in various types of management and commercial the application.

Another embodiment of the present invention relates to following methods: make display window enclose separately bar line code, so that it during as data and information source, is being processed display window in all application programs.

Another embodiment of the present invention relates to following methods: prepare the database that all set up and enclosed the display window of bar line code separately thus, and it is used in Enterprise Resources Planning (ERP) and user's contact management (CRM) application program, with the medicine that is established its database and all commercial networks transaction of sample.

Another embodiment of the present invention relates to a kind of bar line code, display window and any management organization of preparing and controls the method that these medicines shift the database of necessary information at home or abroad.

Another embodiment of the present invention relates to a kind of method of finger-print of chromatogram, and the method can make people understand the physicochemical properties of this medicine such as color and it is carried out standardization, thereby medicine and body fluid are carried out criterion of therapeutical effect.

Another embodiment of the present invention relates to a kind of method of finger-print of chromatogram, the method can make people to this pharmaceutical physicochemistry character for example taste (Rasa) have gained some understanding such as acid (Amla), salty (Lavana), hot (Katu), bitter (Tikta) and puckery (Kashaya) and it carried out standardization, so that these medicines and body fluid are carried out criterion of therapeutical effect.

Another embodiment of the present invention relates to a kind of method of finger-print of chromatogram, the method can make people to the physicochemical properties of this medicine such as hot and cold, effect slowly, effect rapid, heavy, light, soft, lubricated, softening, dry (as at the Guna ' s described in the Ayurveda such as Sheeta, Ushna, Manda, Guru, Laghu, Snigdha and Rooksha) is had gained some understanding and it is carried out standardization, so that this medicine is carried out criterion of therapeutical effect.

Another embodiment of the present invention relates to a kind of method of finger-print of chromatogram, the physicochemical properties that the method can make people understand this medicine are carried out standardization such as the charlotte (being described Veerya, Vipaka and Prabhava) of effectiveness, postdigestive metabolic way and special nature such as molecule and to it, so that medicine and body fluid are carried out criterion of therapeutical effect.

Another embodiment of the present invention relates to a kind of about the 3-D chromatogram of composition and the data processor based on software of profile diagram.

The table, figure, flow chart and the embodiment that set forth certain embodiments of the present invention according to appended being used for describe method of the present invention together, but these contents should not be understood to the restriction to its inventive concept that embodies.

The explanation of subordinate list and figure

Provide by way of example following examples, but these embodiment should not be understood as that limiting the scope of the invention.

The I table

1. be presented at employed different philosophy and the table of various terms in the different medical science

2. the table that shows the relation of body fluid, character and the different parts of human body-Ayurveda method

3. show and close the table that traditional Chinese medical science macrocosm is divided

4. the table that relevant traditional Chinese medical science macrocosm is divided in showing

5. the table of the contact of five kinds of native elements and their relation in the demonstration traditional Chinese medical science

6. the table of the implication of the Yin and Yang in the demonstration traditional Chinese medical science

7. the table that shows the basis of carrying out the standardized color of curative effect of medication

8. show that different colours is to the table of the effect of various disease

9. show the character (Rasas in the Ayurveda) of six kinds of tastes and the table of effect thereof

Color and with the wavelength relationship table

11. show that acidity and salinity are to the table of human body effect

12. show the table that the different analytical technologies that are used for finger-print and chemical standardization are compared

13. show the table for the parameter of setting up the some drugs finger-print

14. be presented at the table of the drug therapy classification of reporting in the invention of this research

15. be shown as the medicine table of sketch

16. show and finger-print entered the partition table of area for treatment according to conjugacy and polarity

Description of drawings

II figure

Tu1A ﹠B has shown in the traditional Chinese medical science five kinds of basic elements and the relation between them. Unbalance (the excessive or shortage) of any element causes other element interference and becomes the basic reason that causes disease. In the traditional Chinese medical science, usually reach the health of human body by the above-mentioned unit of Management and control.

Fig. 2 shows the effect of basic body fluid color, and the basis of this effect is that the medicine of color of the same race is selected for body fluid corresponding to impairment. The color of medicine is to be determined by the chemical property that has its inner composition, so these chemical property are used for criterion of therapeutical effect indirectly.

Fig. 3 has shown the finger-print of the Shilajit of two kinds of different trade marks. The curative effect that shows chemical profile in this finger-print depends on the existence with molecule of wide in range conjugacy more. This chemistry profile along with sample on soil age and change, if the age of sample is larger, then its therapeutic activity is stronger, and its activity depends on the place of collection and the method for purifying.

Fig. 4 has shown the purposes of the chromatographic fingerprinting on the label.

Fig. 5 has shown the chromatogram of turmeric (food).

Fig. 6 has shown the chromatogram of furans azoles pyridine (confrontation therapy).

Fig. 7 has shown the chromatogram of Krimikutara Ras (preparation).

Fig. 8 has shown the chromatogram of Shilajit (effect is good).

Fig. 9 has shown the chromatogram of shilajit (effect is poor).

Figure 10 has shown the chromatogram of Suryavarti (preparation).

Figure 11 has shown the chromatogram of tea (food).

Figure 12 has shown the chromatogram of Trikatu (preparation).

Figure 13 has shown the finger-print of all yellow medicines. Here the finger-print of Sandigha Dravyas (a kind of contrast medicine) shows visibly different outward appearance, and this makes evaluation more easy.

Figure 14 has shown the finger-print of the PITTA HARA medicine that all are natural. The composition that exists in 1 district represents the effect of this medicine.

Table 15 has shown the finger-print of all natural KAPHA HARA medicines. The composition that exists in 2 districts represents the effect of this medicine.

Table 16 has shown the finger-print of all natural VATA HARA medicines. The composition that exists in 3 districts represents this efficacy of drugs.

Figure 17 has shown the finger-print of all natural PITTS KAPHA HARA medicines, represents the effect of this medicine at the composition of 1 district and the existence of 2 districts.

Figure 18 has shown the finger-print of all natural KAPHA VATA HARA medicines, represents the effect of this medicine at the composition of 2 districts and the existence of 3 districts.

Figure 19 has shown the finger-print of all natural PITTA VATA HARA medicines, represents the effect of this medicine at the composition of 1 district and the existence of 3 districts.

Figure 20 has shown the finger-print of all natural TRI DOSHA HARA medicines, represents the effect of this medicine at the composition of Three regions existence.

Figure 21 has shown as the Kali musali of tridoshahara medicine and the finger-print of Safed musali.

Figure 22 has shown the finger-print of different Citrallus Colosynthis samples. Therefore this finger-print shows the shortage of some composition, and this method is used for the extracting method from the same mother liquor tincture of plant is carried out standardization.

Figure 23 shows from the finger-print of different areas with the national different Holarrena Antidyssentric sample of collecting. This finger-print has shown the impact of ecological factor on the plant chemical composition.

Figure 24 has shown the finger-print of two kinds of beetle leaf samples of different regions. The time that flavonoids exists is to show that genotype, phenotype variant and ecological factor were on the impact of plant chemical composition in 30-40 minute.

Figure 25 shows the satellite mapping of India. This satellite mapping shows that there are many tropical areas in India.

Figure 26 shows the finger-print as two kinds of preparations of cosmetics such as herbal medicine shampoo powder.

Figure 27 shows the finger-print of the TRIKATU of two kinds of different trade marks. The variation of composition may be the different reason of their analytical methods among the TRIKATU.

Figure 28 shows the finger-print of turmeric and three kinds of different commercial products thereof. In all these finger-prints, located a common crest at 20 minutes.

(finger-prints of all medicines that Figure 29 reports in 92 display lists 13)

Figure 29 shows two kinds of finger-prints of whole plant of Abel moschus, moschatus medicum.

Figure 30 shows two kinds of finger-prints of Acacia suma bark.

Figure 31 shows two kinds of finger-prints of Acalypha indica leaflet.

Figure 32 shows two kinds of finger-prints of Adhatoda vasaka leaf.

Figure 33 shows two kinds of finger-prints of Adiantum caudatum leaf.

Figure 34 shows two kinds of finger-prints of Ailanthus excelsa stem skin.

Figure 35 shows two kinds of finger-prints of calamus rhizome.

Figure 36 shows two kinds of finger-prints of the large single-lobe bulb of leek.

Figure 37 shows two kinds of finger-prints of the clove of garlic.

Figure 38 shows two kinds of finger-prints of Galanga Galangal Seed rhizome.

Figure 39 shows two kinds of finger-prints of galangal rhizome.

Figure 40 shows two kinds of finger-prints of Alpinia speciosa rhizome.

Figure 43 shows two kinds of finger-prints of Areca Kateeh stem skin.

Figure 44 shows two kinds of finger-prints of uniform female tincture of Ah Buddhist nun chrysanthemum.

Figure 45 shows two kinds of finger-prints of the herb of bacopa monnieri.

Figure 46 shows two kinds of finger-prints of the little Pi's stem of Bhutanese Xiao Hua skin.

Figure 47 shows two kinds of finger-prints of Borrhievia diffusa whole plant.

Figure 48 shows two kinds of finger-prints of the large and ripe fruit of capsicum.

Figure 49 shows the large of capsicum and two kinds of finger-prints of immature fruit.

Figure 50 shows the little of capsicum and two kinds of finger-prints of immature fruit.

Figure 51 shows two kinds of finger-prints of Coscinium fenestratun stem skin.

Figure 52 shows the root of ivy gourd and two kinds of finger-prints of leaf.

Figure 53 shows two kinds of finger-prints of the leaf of actlylactinium Aegyptium (upright).

Figure 54 shows two kinds of finger-prints of the leaf of Dactlylactinium Aegyptium (lodging).

Figure 55 shows the leaf of Diristachis cineraria and two kinds of finger-prints of stem skin.

Figure 56 shows two kinds of finger-prints of the exocarp of Emblica officinalis.

Figure 57 shows a kind of two kinds of finger-prints of face cream preparation.

Figure 58 shows a kind of two kinds of finger-prints of face cream preparation.

Figure 59 shows two kinds of finger-prints of the root skin of licorice.

Figure 60 shows two kinds of finger-prints of licorice whole plant powder.

Figure 61 shows two kinds of finger-prints of gymnema sylvestre whole plant.

Figure 62 shows two kinds of finger-prints of Hollerona Antidysentrica stem skin.

Figure 63 shows two kinds of finger-prints of total shape radia heleii.

Figure 64 shows two kinds of finger-prints of yellow orchid.

Figure 65 shows two kinds of finger-prints of Moringa olifera.

Figure 66 shows two kinds of finger-prints of the female tincture of cis therapy of candleberry.

Figure 67 shows two kinds of finger-prints of Nahi axilae whole plant.

Figure 68 shows two kinds of finger-prints of Oroxylum indicum stem skin.

Figure 69 shows two kinds of finger-prints of holy basil leaf.

Figure 70 shows two kinds of finger-prints that drape over one's shoulders the wealthy Bao Juye of pin.

Figure 71 shows two kinds of finger-prints of Lip river, storehouse Radix picrorrhizae stem skin.

Figure 72 shows two kinds of finger-prints of betel.

Figure 73 shows two kinds of finger-prints of Psoralea corylifolia seed.

Figure 74 shows two kinds of finger-prints of blue beggar's leaf.

Figure 75 shows two kinds of finger-prints of castor root.

Figure 76 shows the stem of R.sylvatica and two kinds of finger-prints of root.

Figure 77 shows two kinds of finger-prints of burdock hieracioides.

Figure 78 shows two kinds of finger-prints of Spheranthus indicus.

Figure 79 shows two kinds of finger-prints of the young tree of pearl stem skin.

Figure 80 shows two kinds of finger-prints of myrobalan's fruit.

Figure 81 shows two kinds of finger-prints of the fruit of Terminalia bellerica.

Figure 82 shows two kinds of finger-prints of whole plant Hu Luba.

Figure 83 shows two kinds of finger-prints of puncture vine stem and root.

Figure 84 shows two kinds of finger-prints of Tylophora asthmatica leaf.

Figure 85 shows two kinds of finger-prints of female tincture of congeneric drug pod.

Figure 86 shows two kinds of finger-prints of Withania somnifera root.

Figure 87 shows two kinds of finger-prints of Zinziber officinalis rhizome.

Figure 88 shows two kinds of finger-prints of Avipattakara churna powder.

Figure 89 shows two kinds of finger-prints of Kamaduga Ras herbal medicinal product.

Figure 90 shows two kinds of finger-prints of the Kumarayasava herbal medicine that is made by fermentation process.

Figure 91 shows two kinds of finger-prints of Mahalakshmi vilas ras herbal medicinal product.

Figure 92 shows two kinds of finger-prints of Suvarna yogaraja Guggulu herbal medicinal product.

Figure 93 shows the some finger-print of Anandabhairayi Ras Right on arbitrary specific peak, and this image software will show that X, Y, R, G and the B coordinate at this peak are to be used for carrying out the encode of bar line. In near square frame (peak), these coordinates have been shown with toolbar.

Figure 94 shows the finger-print of Krimikutara Ras. This image software has shown the bar line code value at specific peak.

Figure 95 shows the bar line code that Anandabhairavi Ras produces.

Figure 96 shows the bar line code that Krimikutara Ras produces.

Figure 97 has shown the display window of Anandabhairayi.

Figure 98 has shown the display window of Krimikutara Ras.

Figure 99 has shown that the database in the establishment that follows the suggested course carries out in the Enterprise Resources Plan and user's contact management application program of networked operation, and how these network work move.

The chromatogram about the Azadiracta tender leaf collected February that Figure 100 has shown that a kind of new bar chart mode with painted represents.

Figure 101 shown that a kind of new bar chart mode with painted represents about medicine Anandabhairavi Ras, a kind of chromatogram of herbal medicinal product.

Figure 102 shown that a kind of new bar chart mode with painted represents about medicine Krimikutara Ras, a kind of chromatogram of herbal medicinal product.

Figure 103 has shown the pie chart of Azadiracta indica chromatogram.

Figure 104 has shown the pie chart of Anandabhairavi Ras chromatogram.

Figure 105 has shown the pie chart of Krimikutara Ras chromatogram.

Figure 106 has shown unusual (dosha) pie chart of quantitative infringement.

Figure 107 has shown unusual (dosha) pie chart of quantitative infringement.

Figure 108 has shown unusual (dosha) pie chart of quantitative infringement.

Figure 109 has shown the 3D of various herbal medicine and the finger-print of profile diagram, with this figure input database and carry out various ERP and the CRM application program.

Figure 110 has shown the 3D of various herbal medicine and the finger-print of profile diagram, with this figure input database and use it for various ERP and the CRM application program.

Figure 111 has shown the 3D of the cosmetic sample that mixes and the finger-print of profile diagram.

Figure 112 has shown the 3D of female tincture and the finger-print of contour images formula, and these figure are used for finding out the dilution of female tincture.

Figure 113 has shown the finger-print of separated medicine of 3D and profile diagram form and their UV spectrum.

Figure 114 has shown the finger-print of cis therapy medicine of 3D and contour images form and their UV spectrum.

Figure 115 shows the operation flow sheet of this fatware.

The specific embodiment

Referring to accompanying drawing, flow chart and embodiment the present invention is described in detail, above-mentioned these reference objects just are used for exemplifying certain embodiments of the present invention, and should not be understood to be the restriction of inventive concept that this paper is comprised.

The chemical standardization method

Therefore the method that provides chromatogram at the single wavelength place from present used is different, has proposed the method for chromatogram standardization, finger-print and the encode of bar line of a kind of new employing profile and 3-D chromatogram. It provides about the overall chemical profile of the middle chemical composition of combination drug such as herbal medicine and preparation or other medicines (such as character such as wherein polarity and conjugation). And the encode of consequent finger-print bar line be can be provided in many business features when adopting ERP and CRM application program that medicine is processed.

The method of the TLC finger-print that is used as chromatographic fingerprinting of Fig. 4 has just shown the analysis to its component at present. It does not provide any chemical property such as conjugacy or polarity. Adopt the finger-print method of HPLC then to show the chromatogram that occurs as " chromatographic fingerprinting " of medicine at the single wavelength place. Wherein, adopt various other analytical technologies such as NMR, LC-MS and the IR that is used for the structure explanation structure at a certain selection peak to be carried out chemical identification. Therefore, under the support that does not have other expensive analytical instrument, the effect that single chromatogram itself can not illustrate medicine how. It is very unpractiaca being used for the technology of this costliness by the various compound herbal medicine of particular treatment purpose and preparations organic and that inorganic drug makes.

The quality of the medicine of any preparation depends on its preparation method. And these preparation methods are different for every kind of pharmacy or every pharmacists. In fact carrying out Quality Control needed to herbal medicine and preparation is a kind of number (quality and quantity) that the component that exists in single medicine or the preparation simply can be provided, and the analytical method of the curative effect of the medicine of studying. It is therefore, any that the method for above-mentioned information can not be provided all is incomplete.

In chemical standardization method of the present invention, at first these components are extracted in the suitable solvent. In high pressure liquid chromatography, under standardized analysis condition, extract is separated into single component. 3-D and outline-color spectrogram that this instrument is given change into chromatographic fingerprinting figure. Being adopted as the special image analysis software of this work analyzes this image. Explain that the result of this output is to carry out this standardization. In description of test of the present invention to the detailed description of the method.

The method of criterion of therapeutical effect

Doctor's Man's Power and sensation are depended in the Traditional Curative Effect standardization to a great extent. Be difficult to obtain the general usage of this method. But present science situation is emphasized and need to and be made it have reappearance with any method or mechanism standardization. Therefore, in the standardized method of chemistry of the present invention and treatment, provide a kind of instrumental method of the people's of reduction sexual factor. This is that a kind of instrument analysis technology is realized its possibility, and this instrument has been described chemistry and the therapeutic efficiency of the medicine of research with simple method. In the scientific and systematical modern science of tool society, should explain the relevant knowledge of estimating the curative effect of medication aspect with ability with rational faculty proof rather than the individual skill with individual difference and non-repeated. The inventive method has been faced this point in the situation of the idea that do not depart from tradition.

As mentioned above, if someone adopts physical-chemical property (polarity and conjugacy) to estimate the curative effect of medicine, then recognize the active of this medicine thereby realized criterion of therapeutical effect. In the methods of the invention, consider to estimate with conjugacy and polarity the curative effect of medicine.

In ancient Chinese prose is offered, according to the physical-chemical property of soil and plant and curative effect and it is clearly classified. Select the medicine of specified disease on the basis of guides such as color, quality, smell and physical appearance. Table 8 is about the effect at the different colours at different human body position, and it has shown how color is used for the object of the invention. This table is also mentioned type and the pharmaceutically-active diversity of soil when selecting medicine. This table is also clearly mentioned effect and its effect aspect the medicinal plant effect of weather. Because being present in endophytic chemical constituent depends on these geology and the Ecological Changes factor, thereby according to the needs of particular treatment effect, formulated about collecting location, acquisition time (season with every day), the plant parts that gathers and the plant age of employing.

Normal conditions according to the plant that is used for the general diseases type, plant is divided into 37 groups of (Wealth of Susrutha, K.H.Krishnamurthy, Indian Institute of Ayurveda, Coimbatore, India-are initially Susruta Samhita sutrasthana 38). Therefore, these plants should contain the chemical constituent that the disease of reporting is had similar curative effect.

When different types of finger-print is studied, find to have common feature about the curative effect of these medicines. In traditional literature, also report this same effect. In other words, coming to the same thing of the result who namely tests and report. Therefore, to the method that studies confirm that of the different medicines with different curative effects.

Figure 13 has shown the medicine that all are yellow. In the ancient Chinese prose of Ayurveda is offered, all these medicines are classified as the Haridra class, the medicine that all are yellow and Haridra (turmeric) are similar. If study its finger-print, can find that then all these medicines are in the news as Kapha Hara, the imbalance of disease is relevant with human body mucogeneous component. Therefore think that the color of medicine is directly related with their curative effect. The color of medicine and effect depend on chemical constituent and their physical-chemical property really in reason.

Single medicine as madder, asoka, Lip river, storehouse Radix picrorrhizae, Phyllanthus Niruri and preparation such as the finger-print of Arogya Vardhini and Avipattakara Churna shown in Fig. 4. According to the elution mode under this group analysis condition, the molecule of wash-out shows the existence of polar compound in 1 district. The general trend of this elution mode that is reported as the medicine of Pitta Hara has confirmed that high polar compound is mainly as Pitta Harra.

Single medicine is reported as Kapha Hara such as the finger-print of Zinziber officinalis (processing), total shape elecampane, burdock hieracioides, holy basil, licorice and shilajit. Show the existence of middle polarity component at the molecule of 2 district's wash-outs. As shown in figure 15, the general trend of this elution mode that is reported as the medicine of Kapha Hara has confirmed that the middle polarity component is mainly as Kapha Harra.

In this width of cloth figure, show single medicine such as Galanga Galangal Seed, castor-oil plant and preparation such as Suvana yogaraja Guggulu, contained Brihatvata chintamani, the Huthasana of swarnamakshkam and the finger-print of Mahayogaraja Guggulu. Show the existence of low or non-polar component at the molecule of 3 district's wash-outs. Observe for this unusual medicine and contain or be mixed with oil. In oiliness class component, find that also the herbal medicine mineral organic metal molecule at this district's wash-out is Vata Hara. As shown in figure 16, the general trend of this elution mode that is reported as the medicine of Vata Hara confirmed low or non-polar component mainly as Vata Harra.

The finger-print that in this width of cloth figure, has shown single medicine such as Azadiracta indica, Curcuma longa, Hollarrheana Antidyssentrica, the little Pi of Bhutanese Xiao Hua, Psoralea corylifolia and Citrullus Colosynthis. Show the existence of high polarity and middle polarity component at the molecule of 1 district and 2 district's wash-outs. Therefore find that these medicines with middle polarity component are Pitta-Kapha Hara. As shown in figure 17, this has confirmed to be familiar with by the polarity of the component that exists in the medicine effect of medicine.

Two finger-prints of single medicine such as puncture vine, Moringa Olifera, betel and preparation such as Trikatu show in 2 districts and 3 districts and have component, and this shows that having effect is Kapha Vata Hara character, and Figure 18 has shown this point.

The finger-print of single medicine such as bacopa monnieri, Oroxylum indicum and preparation such as Kanchanara Guggulu shows in 1 district and 3 districts and has component that this shows that its effect is Pitta Vata Hara character. In a kind of preparation that is called Anadabhairavi, although its effect is reported as Pitta Vata Hara, found Kapha Vata Hara by finger-print. This shows that this artificial preparation can not be successfully used to prepare the medicine of required effect. Therefore, the method is used for standardized method is carried out in above-mentioned compound preparation preparation. Figure 19 has shown this point.

The finger-print of single medicine such as leek, Withinia Pubiscence (black seed), Embalika Officinalis and preparation such as Mahalakshmi vilas ras shows in all three districts 1,2 and 3 and has component, and this shows and has the molecule with total polar scope. This finger-print shows that they have Tri Dosha Hara effect. In the finger-print of Mahalakshmi vilas ras, the molecule of two kinds of similar types of existence may be isomer in nature. When the isomer that has these types (how much with chirality) component, recognize that the medicine of these types has the Prabhava effect. Figure 20 has shown the finger-print of used Tri Dosha Hara medicine.

The finger-print of Kalimusali (Curculigo Orchioidis) and Safedmusali (Asparagus Adescendens) has shown how according to same treatment group two kinds of plants not of the same clan to be classified. As shown in figure 21, these finger-prints have shown the similar components that records in all Three regions, and this has shown its tri-doshahara character.

The single medicine that two kinds of separate sources are provided is such as the finger-print of the Citrullus Colosynthis that uses in Ayurveda and homeotherapy. Observe this finger-print and find that it contains three kinds of polar compounds, but high polar molecule more haply. Examine this finger-print and find that difference between two kinds of images only was to locate to exist or do not exist molecule at 12 minutes. The taste of the first medicine is compared with the second and is wanted much bitter. Therefore also propose to adopt this taste as the tolerance of this efficacy of drugs, as shown in figure 22, this practice is utilized at large in ancient Chinese prose is offered.

Hollarrena Antidysentrica, the finger-print of the medicine that collects from two of this state different areas have shown that their chemical profile diagram has very large difference. This has shown geology, ecology, genotype and phenotype and other changed factor to the impact of the chemical constituent of herbal medicine, and this point as shown in figure 23.

Observe very large difference such as Figure 24 at two kinds of samples of beetle leaf, wherein a kind of sample is from Andhra Pradesh and another kind of from India Calcutta. This has confirmed that ecology, genotype and phenotype change the effect in the chemical constituent of plant parts.

The ecological zone, rainfall, the gas mild climate that have shown India in Figure 25 are to understand season to the effect of its flora and fauna ecology. The variation in season will affect the chemical constituent of herbal plants and by they prepared medicines. This rule is applicable to the whole world, and no matter the herbal plants collection is from the different regions in the world.

Provide two kinds as the preparation of cosmetics such as the finger-print of herbal medicine shampoo powder. The finger-print of this pure herbal medicine material is different from the finger-print of doping fully. Can be clear that on the sample of this doping at 25-40 minute and locate to elute artificial cleaning agent and blowing agent, they have alkalescence and the foaming property of height. This has just supported method of the present invention be used for to be checked at conventional medicament by management organization to mix and contain the copy right piracy of substitute, as shown in figure 26.

The Trikatu that a kind of preparation namely has two kinds of different wave lengths has shown the difference that exists in it is measured. This may depend on the usage that is used to prepare from different sources the single medicine of preparation. The inventive method shows them in the degree of difference qualitatively and quantitatively, so that the medicine of preparation standard and herb extracts, as shown in figure 27.

Figure 28 provides the finger-print of single medicine of three kinds of different wave lengths of same foodstuff such as turmeric. Observing yellow curcumin in the finger-print of this natural turmeric located by wash-out at 20 minutes. In all three kinds of wavelength, all see this same a part. What profile was different is commercial sample, and its reason is that it is that natural turmeric is to be made by the rhizome without processing (not boiling) by processing that (boiling) turmeric rhizome excessively makes.

Figure 29-92 provides the finger-print that various medicines are done, and table 13 has provided the image parameter (front view and rotation diagram) of 3-D and the outline-color spectrogram of all medicines. In table 14, according to curative effect analyzed medicine is classified. Adopt the software of advising that various medicines are carried out fingerprint map analyzing, it will support the inventive method to be used for the practicality requirement of criterion of therapeutical effect.

This has just confirmed the multiple purpose of the inventive method for the treatment of conventional medicament. It is used for making modern pharmacy also understand them in the curative effect of ethnopharmacology.

The method of bar line encode, Enterprise Resources Planning (ERP) and user's contact management are used the application program of (CRM)

In the methods of the invention, this software analysis image also can be by the present invention is based on computer (microchip, Dao Er switch, hardware and software locks) outline-color spectrogram software show the coordinate at the specific peak that this product is all: X retention time, the number of the blue pixel of the number of Y wavelength, the red pixel of R, the number of G green pixel and B. If with these data transaction/be input on its built-in bar line encode software of reselling, wherein certainly can select the pixel value at this image peak, then it can produce the bar line code on " display window " of all details that have been attached to the product of studying.

The present invention is based on computer (microchip, Dao Er switch in employing, hardware and software locks) outline-color spectrogram software when coordinate being provided and adopting software of the present invention that the value of each component is provided, method of the present invention is conducive to any amount of component bar line encode that will be present on the chromatographic fingerprinting of herbal medicine, its this finger-print is with the X retention time, and the blue pixel of the number of Y wavelength, the red pixel of R, the number of G green pixel and B is as coordinate system. Therefore, in the method for this new bar line encode of advising, shown the coordinate figure of this component and other details and be not only the catalogue numeral that is used at present the encode of bar line.

The present invention becomes management organization such as a kind of instrument of the medicine control that herbal products is managed, public analysis, food doping enforement mechanism, court and customs and central national tax department. The finger-print of these medicines should be printed on the label and also should when checking, carry out label to it. It also helps industrial various other wavelength to medicine of the same race to monitor. Figure 93-94 has shown this software is how to provide on the image coordinate of selecting the peak. Provide these to be worth to produce bar line code. Figure 95,96 is consequent line code. Figure 97,98 has shown what kind of the display window of the herbal medicine with the details on the label is. If display window is appended on the bar line code of each product, then this line code can represent this display window. If create the large database of this product and make it available on network, then can any ERP and CRM application program be used for any desired purpose by network. Figure 99 has shown how this network work works in ERP and CRM application program.

Each step that the present invention comprises

In analytical method of the present invention, adopt a kind of high pressure liquid chromatography that is equipped with second order gradient pumping system, photodiode array detector (PDA) of checking and show digital processing unit based on the chromatogram of software. After eluting all compositions, convert 3D and outline-color spectrogram (containing the information about UV-visible spectrum, absorptance and the retention time of all components that exists in single medicine or the preparation) to image, advise being finger-print. It is different from present Pharmaceutical Analysis method that the advantage of this method is, when all components in the medicine being carried out credibly qualitative and quantitative analysis without any need for inside and outside standard items.

The embodiment explanation of the method

With reference to the accompanying drawing, flow chart and the embodiment that are used for exemplifying some embodiments of the present invention, the method for this suggestion is described as 4 steps. These should not exemplified the restriction of regarding the inventive concept that this paper is comprised as.

In following step, whole method is described.

Step 1: select medicine and extraction components.

Step 2: these components are separated into independent component, produce 3-D and outline-color spectrogram and convert thereof into finger-print.

Step 3: adopt the software of developing that this finger-print is analyzed

Step 4: explain (processing) data

The explanation of analytical method of the present invention

Step 1: sample preparation

Chemical property per sample (polarity) selects ethanol to extract component from medicine. When the variation of the PH of hydrous ethanol extract, the extraction of component also changes. Alkalescence PH extracts more component than acid PH. Select the suitable PH that different pharmaceutical is extracted, adopt buffer solution to keep PH. When the PH of selective extraction, the effect of acidity and basicity is taken into account.

Step 2: finish experimental work at instrument

Adopt high pressure liquid chromatography (HPLC) instrument that extract is carried out compartment analysis. In analytical method of the present invention, adopt a kind of high pressure liquid chromatography that is equipped with second order gradient pumping system, photodiode array detector (PDA) of checking and show digital processing unit based on software pearl chromatogram. This extract sample (such as 20 μ l) of known quantity is injected rheology dyne (rheodyne) syringe (being equipped with 20 μ l circulators). Adopt the mobile phase of suitable time-program(me) gradient system under fixing flow velocity (1ml/min), sample to be carried out wash-out. Note any a part of sample not being stayed in the post and not by wash-out. Setting following analysis condition analyzes:

A. adopt the time-program(me) of anti-phase post and water-bearing phosphate buffer solution (the PH scope is 5.5-7.5) to become gradient elution, and adopt a kind of not aqueous solvent (acetonitrile or methyl alcohol) according to the chemical property of the sample of analyzing.

B. adopt the wavelength of 200-800nm and fix the working time according to this time-program(me) at the PDA detector.

The time-program(me) of organic solvent concentration that c. will change the 0-100% of aqueous solvent not such as acetonitrile is applied to the instrument parameter that exists on this instrument.

After in syringe, injecting sample, start this instrument to analyze. When analyze finishing, instrument is quit work or after the All Time program is finished this instrument automatically quit work.

In the data of three types show, a window of this chromatogram has shown at the chromatogram of selecting the wavelength place, another window has then shown its outline-color spectrogram, this outline-color spectrogram has shown the retention time (working time) of this analysis in X-axis, and has shown the scope of wavelength in Y-axis. The 3-D chromatogram that has shown sample in another window has wherein shown in X-axis to have shown the retention time (working time) of this analysis concentration range and shown wave-length coverage at Z axis in Y-axis. 3-D and the outline-color spectrogram that will so obtain by this system convert image to.

Adopt the software advise that the image that obtains is thus analyzed, this software provides the data of new qualitative and quantitative analysis about the chromatogram that has composition in this medicine. Adopt different color violets, indigo-blue, blue, green, yellow, orange, the red pixel value that represents, the character of these colors is the tolerance as the concentration (quantitative) of the component that is directly proportional with color. Select the demonstration of each window of above-mentioned shades of colour and every kind of color. The method of Here it is chemical standardization. In Figure 100-102, shown the chromatogram that some obtain thus.

The chromatogram that is so obtained by this software provides the conjugacy (shown in the UV-Vis absorptance) of each component and the information of polarity simultaneously. According to used post and mobile phase this image is divided into Three regions and shows, namely based on 1 district (high polar region), 2 districts (middle polarity district), 3 districts (the low or nonpolar district) weighed by retention time of this elution mode. The counter-rotating analysis condition can make elution mode reverse.

Utilize the three-dimensional nature of described image that the 3-D chromatogram of this medicine is analyzed. If regard this 3-D chromatogram the cap of band cover as, and the sample of whole cap and another kind of different quality and quantity character is carried out three-dimensional match, then the degree that matches can be expressed as qualitative and quantitative analysis report. Here this calotte is compared with the molecule peak of certain wave strong point. It is the cap with a plurality of covers that sample with greater number peak appears to. Therefore carrying out three-dimensional coordinate matches the method for foolproof comparison and analysis is provided. Its coordinate that matches has provided the qualitative data of the sample of studying, and its degree that matches has then provided the quantitative data of institute's study sample. The specific software that is adopted as this purpose and establishes can realize the method. This method can become a kind of quality control method.

The polar interaction of separated molecule, the fixedly polarity of phase and the elution mode that the polarity that is used for the mobile phase of elution samples has determined these molecules. The comprehensive interaction of all these three kinds of parameters relevant with other such as temperature etc. has determined elution mode and based on the eluting order of the component of its polarity. Therefore, polar molecules all in medicine will be in " 1 district " (doping region of this image) by wash-out, all middle polarity molecules will be in " 2 district " (middle polarity district of this image) by wash-out and all low polarity or nonpolar molecules will be in " 3 district " (nonpolar district of this image) by wash-out. In these three districts of many finger-prints, elute minute period of the day from 11 p.m. to 1 a.m chemistry and the therapeutic efficiency of this medicine carried out many summaries. These zones show such as mark among Figure 103-105.

Therefore, this chromatogram provides medicine is how to bring into play chemistry and the information of acology effect. If adopt image or data display method to show each component that exists in each district, then it has provided whole components in each district to the percentage of specific dosha effect. Therefore, this data interpretation its (medicine) as the character of the quality of the component that how wherein exists and quantity be the basis jointly therapeutic action in the infringement of every kind of dosah. For example, if this medicine has 30% component in high polar region (with the shades of colour of specific region such as green, yellow, orange and red amount of pixels as quantity), 70% component is arranged in the middle polarity district, wherein color represents the variable concentrations in the finger-print, and then it shows as a kind of 30% and acts on the medicine that pitta and 70% acts on kapha. Thereby can be Pitta-Kapha Hara (30-70%) with this drug evaluation. Therefore with the infringement quantification of dosha. This helps to make the doctor to understand the effect of this medicine and determines its consumption. Some embodiment circular diagram is shown in Figure 106-108.

Adopt the analysis condition of reporting to make 3-D and the rim(ming) light spectrogram of the herbal medicine of reporting. The sketch of this medicine has represented how to adopt software that this finger-print is processed, as this software of employing is processed the somatic fingerprint collection of illustrative plates. All these features finger-print etc. as seeking similar finger-print and comparing class can be finished by introducing required software features. In Figure 109-114, provided the finger-print sketch of different pharmaceutical. The inventory of the medicine that shows with the finger-print form is as shown in Table 15.

Step 3

Adopt the image analysis software analysis image

After all compositions are by complete wash-out, convert 3D and outline-color spectrogram to image and the finger-print of advising. It is different from the analytical method of synthetic drug that the advantage of this method is, when all components in the medicine being carried out credibly qualitative and quantitative analysis without any need for inside and outside standard items.

After the 3-D that makes the medicine of studying and outline-color spectrogram images, therefore (be called as chromatographic fingerprinting), the software that adopts the color of the quality of the component that being used for of advising exist various representative medicines and quantity character to analyze is analyzed.

Because this image can not become the analysis data of science; thereby developed a kind of computer based image analysis software (software and hardware protection) and analyzed this image and according to being present in the various retention times of this image and the color of the component in the pixel value, provide the proportional concentration of the composition of the medicine of studying.

At present can provide finger-print by above-mentioned image analysis software. Finish by the following method versicolor analysis: represent these components with the peak in the chromatogram, thereby show for the new chromatogram that goes out with the form of bar chart. It has shown that all are by the number of the compound of elution fraction and their conjugacy and UV-Vis absorption properties. In the technical characterictic of this software, discussed the method that comprises in this graphical analysis in detail.

The scale that the chromatogram of the bar chart form that derives thus provides retention time (0-60) is on the X-axle and the chromatogram of wavelength on Y-axis of 200-800nm scope. This image has provided by the number of the pixel of the color filling of every kind of composition, so that each component that is present in the medicine is carried out qualitative and quantitative analysis. Therefore resulting chromatogram represents to be present in number and the UV absorption region thereof of the component in the medicine, and wherein the quantity of pixel is directly proportional with the concentration of molecule.

If according to the elution mode of molecule and the change in polarity of mobile phase image is divided into three districts, then 1 district is doping region, and wherein used post is the anti-phase post; 2 districts are for eluting the middle polarity district of middle polarity molecule; And 3 final districts are low or nonpolar district, wherein elute molecule nonpolar and very low polarity in this district. Therefore, the molecule of wash-out is polarity in 1 district, is middle polarity at the molecule of 2 district's wash-outs, and is very low polarity or nonpolar at the molecule of 3 district's wash-outs. Therefore, three districts of this image have provided the polarity of the component of all wash-outs.

On the basis of the polarity of the molecule of wash-out, medicine is classified by system according to Traditional Curative Effect, finds that wherein polar compound is Pitta Hara, and the middle polarity molecule is that Kapha Hara hangs down polarity or non-polar compound is Vata Hara. Here it is the standardized basis of this curative effect of medication. Polarity and the continuous emission spectrum of these components are compared, wherein dosha is divided into the acute to chronic of every kind of dosha. The section start in this district is acute and chronic in the end representative in this district. Therefore the compound that occurs in described district will act on the disease of described intensity.

Table 16 has shown that the color according to absorption is divided into different treatment regions with polarity with this finger-print. Scale on the X-axis represents based on the polarity of the molecule of mobile phase polarity size, and the Y-axle represents the wave-length coverage (200-800nm) that absorbs. According to physical-chemical property (color and chemical property) and the experiment of the curative effect of reporting in the document, medicine, with the criterion of therapeutical effect of various medicines. Because as a result some deviation is found in the effect of various such environmental effects pharmaceutical chemistry components.

Therefore, the method will help to understand the curative effect of the medicine of studying. So the method for advising will become the visible evidence of curative effect of the medicine of medicine that understanding reports or new, single or preparation.

Graphical analysis is to use software for this purpose to carry out. The details of software see to mark and Figure 115.

Step 4: explain this data

The finger-print that analysis produces is to be used for explanation chemistry and therapeutic properties. The essential characteristic of finding finger-print is:

1) the eluted doping region of component; With

2) conjugacy that shows of each component

The polarity of post is fixed. It is the fixedly phase of normal phase or anti-phase. Fixing in the normal phase post is nonpolar for polarity is fixing in the anti-phase post mutually. Even in the anti-phase or normal phase post of same type, each fixedly the polarity degree of phase become with each trade mark. Utilize polarity, additive such as the buffer solution of mobile phase to control this fixing polarity mutually with PH. If mobile phase polarity often changes with the order that increases and reduce on the anti-phase post, then the component in the sample with identical order by wash-out, namely elute high polar compound by high polarity mobile phase, the middle polarity mobile phase will elute the component of middle polarity and elute non-polar component by nonpolar or low polarity mobile phase. Highly preferred pattern is for changing the polarity of mobile phase by the order that increases or reduce polarity, thereby is left in the post not by wash-out without any the component of polarity, and then obtains fully wash-out. Therefore, thus the polarity of phase will make mobile phase bring required effect to obtain the separation of required eluting order to the polarity of these components by controlling fixedly.

The order of polarity and wash-out and character go for the situation of anti-phase post in normal phase post situation, except the anti-phase post being reversed. According to the order of wash-out with mobile phase polarity, non-polar component is wash-out at first, then is polar compound.

Therefore, polarity that can also be by the control mobile phase in wash-out of the present invention and change successively the order of mobile phase and control and start the wash-out of component with required pattern.

Generally from high polarity mobile phase to low polarity mobile phase sample is carried out wash-out. Therefore, in finger-print, the component that is positioned at the first district (1 district) will be high polarity. Same pattern is applicable to other district, elutes the middle polarity component and (3 district) elutes low or non-polar component in nonpolar district in middle polarity district (2 district).

Most high polar molecule is high chemism, thereby biologically, if they enter the oral cavity, the first position of digestive system, then they will begin to act on biosystem and the enzyme of there immediately. Then this component will enter stomach and intestines, owing to have herein digestive juice and their enzyme, thereby they will experience different variation (digestion afterreaction, the Vipaka in the Ayurveda). In this absorption process, high activity (high polarity) molecule will begin to interact with biosystem and demonstrate its therapeutic properties at this moment immediately. This can make comparisons with the following fact, and as in Ayurveda, the belly of human body is classified as the Pitta district, and wherein high polar molecule has played main effect. Heat production mechanism will will play important effect in disease and the biomechanism of being correlated with. It shows that indirectly highly active molecule is high polar molecule.

After absorbing, the blood that contains absorbed component takes component to heart and reaches relevant thereafter position. Then blood will be sent to each different parts of human body. In Ayurveda, the top of human body is called the Kapha district, wherein cold mechanism will play Main Function. Therefore, the molecule of middle polarity plays important effect in the mechanism relevant with this district.

Therefore only can enter human body by the blood transmission by low polarity and non-polar component, belonging to will become last polarity kind by the human organ that blood obtains the mechanism of chemical constituent. With non-polar oil in the human body, fat and other this molecule and mechanism thereof classify as the vata disease, and the material of adopting same type is treated this disease.

Final area at this finger-print will elute low polarity and non-polar component. Therefore, should distinguish (3 district) and be called the Vata district. Therefore, according to its saltiness body fluid of polarity identification of these molecules, this helps to understand it will act on which type of disease (dosha). Therefore, the present invention is used for the criterion of therapeutical effect of medicine.

This image is divided into three districts in X-axis and Y-axis. Conjugacy (absorption of specific emission wavelength) is marked on the Y-axis, and polarity is marked on the X-axis, wherein adopt the polarity of mobile phase composition to control the wash-out of component. As described in document, basis is carried out scale based on the curative effect of wavelength (color) on Y-axis. Whole image is divided into the cell that six chemical constituents wherein have specific conjugacy and polarity. This further is directly proportional with the curative effect of component in this cell again. Therefore, if according to the performance specific wavelength absorb and color with particular polarity with the medicine finger-print, then calculate in whole colors in this district and the curative effect used it for the component that exists in this medicine and make an explanation. Therefore, adopt the method to realize whole criterion of therapeutical effect and chemical standardization. The diagram expression is such as table 15, and this table has shown the relation of different component curative effect in conjugacy and polarity and the medicine.

If adopt all 3 dimension character of the image of this medicine it is carried out the analysis of 3-D chromatogram, if and this 3-D chromatogram is regarded as the cap of band cover, and the sample of whole cap and another kind of different quality and quantity character is carried out three-dimensional match, the degree that then will match is expressed as the qualitative and quantitative analysis report. Here the molecule peak of this calotte with the certain wave strong point compared. It is the cap with a plurality of covers that sample with greater number appears to. Therefore carrying out three-dimensional coordinate matches the method for foolproof comparison and analysis is provided. Its coordinate that matches has provided the qualitative data of the sample of studying, and its degree that matches has then provided the quantitative data of institute's study sample. The specific software that is adopted as this purpose and establishes can realize the method. It can become a kind of basic quality control method.

But any not carry out quantitative method all be useless. Therefore, think that in the image of a certain specific region whole colors of all components are the expression of the polar compound quantity that exists in the medicine. Therefore to represent that this medicine is illustrated in district-1Pitta district, district-2Kapha district, all components that the district-the 3Vata district exists to the form of the circular diagram of the effect of various diseases. Therefore, the order that contains component is that 50: 20: 30 medicine is that order is 50%: 20%: 30% Tridoshahara medicine. Therefore with the curative effect quantitative criterion. Can increase or reduce any or two kinds of other Doshas by adding the medicine preparation means such as other medicines and the needed suitable formulations of preparation treatment particular individual.

This chemical standardization is performed such: utilize this software, according to the color of indication constituent concentration each component is carried out quantitatively. The wave-length coverage indication conjugacy of molecule absorption.

As described in traditional standardized method, according to color and the curative effect of medicine their color is carried out standardization. Even it is applicable to the situation of molecule. To explain how color is used for the curative effect of medicine is carried out standardization about the table 8 of color and curative effect thereof. Can be understood by the emission absorbent properties of the UV-VIS transmitting boundary of medicine their color. About color and with the table 10 of the relation of wavelength in, color and the characteristic wavelength thereof of medicine are provided. The structure of medicine, functional group, conjugacy and unsaturation will affect absorption (absorption maximum) wavelength of molecule. If molecule is in conjunction with absorbing wavelength will be longer more at most. Therefore, the UV-VIS of any molecule absorbs the quantitative and qualitative analysis that has been widely used in component.

In offering, ancient Chinese prose provided color and the curative effect of various medicines. The color of molecule depends on the specific chemical property of this molecule. The color of combustion flame has been used to the quality control to metal and Related product thereof, and this method has comprised basic spectroscopy principle. Therefore with the relevant interactional research of Electromagnetic Launching and knowledge for chemical property and the curative effect of medicine are studied. This principle has been used in finger-print of the present invention and the standardized AAS. On the other hand, with a kind of form of new analytical method existing concept is expressed, removed the mistake of people's sexual factor. In table 14, provided these all medicines, and provided by the finger-print of its generation curative effect table by this medicine. The ins and outs of this software are provided by the issue explanation of this software.

The issue explanation of suggestion software

1) system requirements (minimum)

A. processor: Pentium II or higher

B.OS:Windows 95, Windows 98, Win NT 4.0 and Linux

C.RAM:64MB or higher

D. monitor: 14 " colour picture monitor (1024 * 768) or higher

E. software: Java developing instrument (JDK1.2.X)

II) operating principle of this software

Different operating principles is as described below: the selectable order with various functions shown in Figure 115.

Software titles: rainbow (a kind of image analysis software for chromatographic fingerprinting)

The software of developing is used for chromatographic fingerprinting and micro-image

1. it is based on the software of GUI (graphic user interface).

2. this Software for Design is used for the image of any kind is analyzed, and especially chromatographic fingerprinting is analyzed.

3. the form with chart provides report

4. lifetime

A. input: image

B. process:

Analysis comprises:

Extract color (7 kinds of colors and some their different shades of standard)

Adjust size, be divided into three districts 20 minutes the time interval

Mapping (bar shaped and circular diagram)

The encode of bar line

Extract the standard of color institute foundation:

This software extracts 8 kinds of colors, i.e. red, green, blue, Huang, green grass or young crops, fuchsin and orange.

Any color is not absolute. It is the mixture of the shade under the color before and after it, and they change in a value range. The scope of these colors is used for differentiating the color that provides more than the conduct that its value separately is from international standard 256-tint volume. The value that adopts in the software of the present invention is:

About red color

Red bluish-green

200-225 0-64 0-64 and

192-200         0-64        0-32

About green color

Red bluish-green

0-64            0-48        200-225

0-65            0-64        65-191

About blue color

Red bluish-green

0-96            200-255     0-191

Similarly, with the standard of other color as the extraction color. (these standards meet the specificity requirement of software of the present invention, and if need it to be updated).

When image was analyzed, this software was read each pixel of this image, and read and extract color according to the color standard of appointment, they was stored and change to be further used as bar chart to show.

C. output

Report:

1. pass through figure

2. by save data such as image, figure, image shown be divided into three districts

3. by showing X (pixel value of retention time and image), Y (wavelength or the absorptance of each profile and 3-D chromatogram image), R (redness), G (green) and B (blueness) coordinate.

4. by these values being converted to built-in bar line encode software to produce bar line code.

D. user interface: allow the user by the whole bag of tricks and product interaction

1. input required image (one or more)

2. adjusting image analyzes to required size and to it

3. the preservation image is adjusted the image size, and it is carried out diagram

4. print this image, adjust the image size, and it is carried out diagram

III) technical characterictic of this software

1. this software is called " rainbow "

2. this software has following functions: different-format in the opened file folder (extension name) resembles the chromatographic fingerprint image of .BMP, JPEG, TIF, GIF and with single pixel sensor the different color that exists in the image is analyzed;

3. this software has the function with such form display pixel information: 1. (0 (minutes specification) and Y (200-800nm) coordinate the image of specification to have X, with 2. except this figure, the cake formula logarithm (automatic and manual) of the independent numerical value at each peak in two kinds of splitters;

4. this software has the function of using the PRINT icon can print all data of the generation after the analysis;

5. this software has the function of using PAGE SETUP icon can change the page setup of printing;

6. this software has the function of using the RESIZE icon can the selection portion partial image and analyze;

7. this software has the function of different images being opened the graphical analysis window of any number, and with WINDOW icon show state;

8. this software has the function of using the ZONE icon can be divided into image at 20 minutes intervals three kinds of zones;

9. this software has the function of using the INVERT icon can make the Image Reversal of selection;

10. this software has the function of using the EDITOR icon can switch to Notepad, Word pad and MS Word;

11. having, this software use the HELP icon can show function about the operation information of the various features of this software; With

12. this software has the function of using SAVE AS icon can store according to the .JEPG file format data that produce.

IV) installation instructions of this software

A. the installation process of the workbench Java 1.2.x software of software of the present invention.

● seek Java CD-ROM.

● double-click jdk1.2.0/jdk1.2.1/jdk1.2.2 icon is installed.

● installation is with extendfile and allow the user be confirmed whether to load this software in system.

● hit ' yes ' software and put question to and which catalogue this document will be installed on.

If ● select acquiescence, then will show c: the jdk1.2 catalogue.

● if you want to be installed to " d " dish, chdir and this software is installed.

● install one and finish, forward c to: and open the file that is named as ' autoexec.bat '.

● in this autoexec.bat file, provide following path:

●set path＝d:\jdk1.2/bin:％path％

●setup class path＝d:\jdk1.2\lib\classes.jar；％classpath％

● restart and use.

The installation of the image dissector software of b. advising

1. from CD, the file of this image dissector software is copied to the target directory of system

2. in the file that copies image dissector software, seek the batch file.

3. right click this document is also clicked ' send to desktop as short cut '

4. ' the icon shortcut of MS dos ' appears on the table. This icon of right click also forwards attribute to, and the option program table also checks ' close on exit check box; Window state is converted to ' To minimized '

5. use and close.

6. the image dissector software preparation is ready for use now. Double-click this image dissector icon, it is just started working.

7. on the window of opening, a square frame that indicates ' CSIR ' will be opened, and answer therein key feeding cipher ' dvk '

8. hit arrow mark (hand) to open this software in this lower right corner of opening image.

9. open the image directory that does not have graduated contour images, the image that selection will be analyzed. This image will show in image window.

10. hit the RED analysis window that is marked with red frame. One width of cloth circular diagram will be with a width of cloth X-axis mark retention time and the chromatogram of Y-axis marking nano shows together.

11. for the component of lower concentration, hit green, yellow and orange. Other color is roughly baseline or small concentration but can not be left in the basket more.

Use this software further feature details 12. under the help menu of this software, provide, comprise various features and the application of this software.

V. known defective:

Do not find

VI. used abbreviation

The a.JDK:Java developing instrument

B.Con: outline-color spectrogram

C.3-D:3-tie up chromatogram

D.WOS: do not have scale

E.X: the retention time that represents chromatogram

F.Y: the wave-length coverage of representative on the absorptance on the 3-D chromatogram and outline-color spectrogram

G.R: at the red color of specific location of pixels

H.G: in the green colourity of specific location of pixels

I.B: at the chroma blue of specific location of pixels

VII. the implication of various icons and function

All data that the a.PRINT icon will produce after will being convenient to print and analyzing

B.PAGE SETUP icon will be convenient to change the page setup of printing

The c.RESIZE icon will be convenient to select the part of image and the selection portion of image will be analyzed

The d.WINDOW icon will be convenient to open the graphical analysis window of the different images of any number, and show therein its state

The e.ZONE icon will be convenient within 20 minutes the time interval this image is divided into three districts

The f.INVERT icon will be convenient to selected image is changed

The g.EDITOR icon will be convenient to switch to Notepad, Word pad and MS Word.

The h.HELP icon is with the operation information of being convenient to show about the various features of this software application.

I.SAVE AS icon will be convenient to preserve the data that produce with * .JEPG file format.

VIII. limit to:

A) software of the present invention is only to carrying out work without scale profile chromatogram.

B) (" dividing " represent the retention time of this outline-color spectrogram) expression with 1 of the scale on the X-axis.

C) scale on the Y-axis is 200-800nm, the wave-length coverage that representative is analyzed.

D) must adopt image software with the Image Adjusting that produces to X and Y-axis on working time and wave-length coverage match.

E) image after the analysis can only save as and not have graduated JEPG form.

F) image of shearing can only adopt the coordinate system of expansion to preserve

Major advantage of the present invention:

1. the outline-color spectrogram of medicine becomes its finger-print. Therefore it contains relevant for the concentration of composition and the UV-VIS spectral wavelength of molecular polarity. Produce medicine of the same race and help the distribution of understanding in the intestines system of medicine at different PH at the finger-print that extracts under the different pH values, thereby promote the drug effect of institute's drugs.

2. on single width figure, provided easily the spectral wavelength of all components of estimating this drug therapy attribute and character.

3. this 3-D chromatogram becomes the UV-spectrum picture of all wavelengths of each component on the single width figure of chemistry (conjugacy and the polarity) attribute that shows the wash-out molecule.

4. be used for controlling for public interest using and abusing of herbal medicine about the finger-print data of the existing various herbal medicine of this country by quality control amount, court and department of customs.

5. this database also provides pharmaceutical value (acology classification) about the various medicinal plants of this state and ecological factor to the information in the effect of this same plant of the different tropical area of this state. The herbal medicine that this is conducive to select the medical expert of quilt that suits or herbalist to treat application.

6. adopt this finger-print of this software analysis that the effect of ecological factor to these available various herbal medicine of state has been described, and by quality control amount, court and department of customs it is used for controlling for public interest using and abusing of herbal medicine.

7. be used for adopting the physicochemical properties of medicine to understand the curative effect that the medicine of reporting offered in ancient Chinese prose to the analysis of finger-print.

8. this analysis gives the pharmaceutical value of these various medicinal plants of state and ecological factor to the information of the effect of the chemical composition in the medicine of the same race of these state each department.

9. acology and the ethanol-plant classification of finger-print are helped to carry out some summary, and these are summarized for making doctor and researcher understand conventional medicament fully by the analysis of finger-print.

10. by with the encode of image attributes bar line, make these medicine/plant extracts/plant not by piracy, because it is conducive to by the notepad instrument, utilize the attribute of this image to set up bar line code.

11. in all business transactions of modern ERP and CRM are used, use this line code.

Application practicality of the present invention

In the world

This finger-print can be used and be used for by any one country the patent of this state's conventional medicament of application. Reason is, because the variation of chemical profile, the finger-print of the kindred plant of the finger-print of single medicinal plant and other area and country is not similar. The causes of change of chemistry profile is that variation, soil, water quality and the genotype of ecological factor such as tropical area and phenotype changing factor are on phytochemical impact.

This method helps this state to fulfil the regulation that WHO proposes, because member state will be with application and method of quality control and their management standardization of herbal medicine.

Domestic

Show that by making laws " medicine that produces this finger-print is national wealth with plant " makes it as a kind of instrument be used to preventing international conventional medicament piracy. If if medicine is applied for Anywhere that in the world patent of any type and its finger-print and the finger-print of the medicine of the country that is challenged coincide, then this patent is out of court.

Strategy

The finger-print bar line encode of medicine is helped medicinal plant is carried out authority's management and protection.

Convert the bar line code of the finger-print of medicinal plant to a kind of machine readable language, then commercial and management work will become easy.

The finger-print of medicine helps to make food and medicine administrative organ, the management when this medicine immigration of license of customs and central national tax department, checks the use of domestic herbal medicine, abuses and usurp.

Industrial

The medicine that produces or the finger-print of preparation help the industrial process technology of protecting them by the finger-print of the medicine of the same race of other trade mark relatively. Therefore it helps to make the enforcement of Patent Law more effective.

It is how to change its pharmaceutical properties that this finger-print helps by monitoring these medicines at the different processing stages adding other medicines of preparation preparation.

The industrial fingerprints database that can utilize these domestic all plants of state that create is selected the area of medicine and is gathered. It helps the industry of the countries and regions in the suitable season that gathers herbal medicine, because ecological factor has changed the curative effect of medicine.

On the science

The method helps to make the researcher to understand prepared conventional formulation. It also helps to monitor prepared novel formulation.

It helps to understand how the recruit forms when preparing compound conventional formulation.

The finger-print of the medicine of the same race that produces under the different PH helps to understand medicine drug distribution at each different PH place in the intestines system.

The outline-color spectrogram of medicine becomes its finger-print. Because it has comprised UV-Vis spectral wavelength about the polarity of the concentration of composition and molecule.

If in single width figure, provide the spectral wavelength of all components, then be very easy to the therapeutic properties of medicine is estimated.

This 3-D chromatogram becomes the UV-spectrogram of all wavelengths of each component on the single width figure of chemistry (conjugacy and the polarity) attribute that shows the wash-out molecule.

This database also provides about the information of the pharmaceutical value of the various medicinal plants of this state (acology classification) and ecological factor the effect at this same plant of the different tropical area of this state. The herbal medicine that this is conducive to select the medical expert of quilt that suits or herbalist to treat application.

Acology and ethanol-botany classification to finger-print help to carry out some summary, and these are summarized for making doctor and researcher understand conventional medicament fully by the analysis of finger-print.

The inventive method is conducive to create the chromatographic fingerprinting of herbal medicine and preparation, and it is used for many Quality Controls and administrative purposes.

Method of the present invention has promoted described medicine is carried out chemical standardization (quantitative and qualitative analysis) by conjugacy and the polarity that provides the various molecules that exist in medicine with UV-VIS absorbent properties and any organic or organic-metallic compound. This alanysis is widely used in carries out chromatography in fact not obtaining its single herbal medicine and preparations outside and the internal standard product.

The present invention is conducive to the curative effect of the medicine of studying is studied, understood and monitors. It helps to understand therapeutic action and the character that the middle conventional medicament of reporting offered in ancient Chinese prose, and has confirmed this point in the mode of reproducible analysis data. Therefore it provides the medicine to studying to carry out criterion of therapeutical effect. It has shown polarity, middle polarity and the nonpolar molecule that exists in doping region such as the sample, thereby is conducive to understand the overall efficacy of medicine.

The medicine that the method is conducive to report carries out again standardization, thereby makes it meet the needs of present treatment. It helps to monitor and study the new formation with the organic of UV-VIS absorbent properties and organic metal molecule in the process of preparation preparation that report or new. It also helps will prepare process technology standardization that reported and new preparation by monitoring component and different chemistry thereof and therapeutic properties.

It is conducive to produce bar line code by a kind of built-in bar line encode software, and wherein this software has provided following coordinate: the number of the number of X retention time, Y wavelength, R red pixel, the number of G green pixel and B blue pixel. The embodiment of the bar line code of chromatogram is provided. The present invention also is conducive to one or more the component bar line encode that will be present on the finger-print, thereby promotes the business transaction of adopting Enterprise Resources Planning (ERP) and user's contact management (CRM) application program to carry out. The database that makes thus becomes the firsthand information of ERP automatic vending machine or other kind. All details that this machine will show this medicine are the company, its chemical fingerprint and curative effect thereof of medicine as described. This is so that the evaluation of this machine is more more credible than now.

The fingerprints database that adopts the method to obtain helps to carry out the summary of many curative effects about particular treatment group plant. Therefore, one will understand that why specific plant is added to that group. Table 14 has carried out detailed explanation to this.

The finger-print that the label of this machine is printed help to make the doctor understand before used medicine curative effect and confirmed every a collection of medicine is carried out Quality Control.

Will about the analytical applications of the chromatographic fingerprinting image (outline-color spectrogram) of the different pharmaceutical of any subject (single medicine or preparation) in as the different step of this application described in multiple purpose.

Society

The single medicine that it is used for that the consumer is understood and requires on the label or the curative effect of preparation also confirm that it contains the thing of the requirement on the label.

The quality control of the herbal medicine that it helps that the consumer is supervised sells on the market and the interests of Protection of consumer.

Mix

To mix for detection of any medicine about the analysis of the chromatographic fingerprinting image (outline-color spectrogram) of the different pharmaceutical of any subject (single medicine or preparation).

Table 1: about the different subjects that adopt in the medical science and the table of different terms

SL No.	Subject	TRI DOSHA(Hara)	BANCHA BHUTA	Character	SAPTA DHATU	TRI MALAS
								1	Ayurveda (describing in detail respectively such as each table) binary: Prakriti-Purusha	vata， Pitta， Kapha	1.Prithivi 2.Ap 3.Teja 4.Vayu 5.Akasha	1.Rasa-taste 6 2.Guna-character-20 3.Veerya-render a service-2 4.Vipaka-Metaboki te-3 5.Prabhava-characteristic-countless 6.Karma-Act ion basically	1.Rasa 2.Rakta 3.Mamsa 4.Medas 5.Asthi 6.Majja 7.Shukra	1.Purisha 2.Mutra 3.Sweda
2	Siddha binary: Prakriti-Purusha	Pitta， Kapha， vata	1.Mann(Prithvi) 2.Neer(Ap) 3.Thee(Agni) 4.Vayu 5.Akasha	1.Rasam 2.Gunam 3.Veeryam 4.Vipakam	(1.Rattam blood) 2.Sadhai (muscle) 3.Kozhuppu (medas) 4.Elumbu (bone) 5.Vomdji.Lari (seminal fluid, ovum)	1.Malam 2.Mutram 3.Vervai
							3	Traditional Chinese medical science binary: male-female	1. cloudy 2. sun	Wood	2. the fire 3. native 4. Jin5.Shui	1. acid 2. bitter 3. sweet 4. hot 5. is salty	1. sinus 2. blood vessels 3. muscle 4. hairs 5. bones	There is not available information
4	Tibetan medicine	Nes pas 1.Mkhris(Pitta) 2.Bad-Kan(Kapha) 3.Rlun(Vata，Vayu)	1.Sa(prithvi) 2.Chu(Ala) 3.Me(Agni) 4.Rluin(Vayu)	There is not available information	Lus Zuns Bdun 1.Dans Ma(Rasa) 2.Khrg(Rakta) 3.Sa(Mamsa) 4.Tsil(medas) 5.Rvs(Asthi) 6.Rkan(Majja) 7.Khu Ba(Shukra)	Dri Ma 1.Bsan 2.Gcin 3.Rnul
							5	Unani binary: normal-unusual	Akhalat 1.Damv (blood) 2.Balgam (phlegm) 3.Safrayi (bile) 4.Saudai (Vata)	Arkan 1.Aag (fire) 2.Hawa (air) 3.Pani (water) is soil (Mitti) 4.	1.Garm (heat) 2.Khush (does 3.Sard (trembling with fear) 4.Motadil (nature)	1. basic: blood, phlegm, bile, Saudai 2.Mahsoora (in the blood vessel) 3.Qureeba (intercellular) 4.Muviya (cell) human organ monomer, compound	1.Bole 2.Baraj 3.Paseena
6	The reverse medicine of Greece	1. jaundice juice 2. is deceived bile 3. phlegm 4. blood	1. water 2. soil 3. fiery 4. air	1. heat 2. dried 3. wet 4. is cold	1. oiliness 2. coarse 3. heat 4. are cold

Table 2: the relation of body fluid, character and human body different parts-Ayurveda method

S1     No.	TRI  DOSHA  (Hara)	TRI  MALAS	PANCHA BHUTA (physical property)	SAPTA  DHATUS	Chemical property	The relation of MAHABHUTA and DHATUS	Because DHATUS and to the effect (reducing this DOSHA) of DOSHAS	Relation with GUNA	Relation (absorption aftereffect) with VIPAKA
										1.     2.     3.	Vata.  Pitta.  Kapha.	1.Purisha  2.Mutra  3.Sweda	1.Prithivi  2.Ap  3.Teja  4.Vayu  5.Akasha	1.Rasa  2.Rakta  3.Mamsa  4.Medas  5.Asthi  6.Majja  7.Shukra	1.Rasa (Shadruchi ' s a.Madhura b.Amla c.Lavana d.Katu e.Tikta f.Kashaya 2.Guna-: broadly be divided into 3 groups of 1.Vaisheshik 2.Samanya 3.Atma and usually use: Guru (weight) Laghu (gently) Sheeta (trembling with fear) Ushna (heat) Snigdha (soft, smooth, softness) Rooksha (thousand) Manda (slowly) Teekshna (urgency) 3.Veerya-2 4.Vipaka-3 5.Prabhava-	a.Prithivi+Ap b.Agni+Prithiv e c.Jala+Agni d.Aksha+Vayu EAgn i+Vayu f.Prithive+Vay u	a.Pitta Vata Hara b.Vata Hara c.Vata Hara d.Kapha Hara e.Kapha Pitta Hara f.Kapha Pitta Hara	a.Furu，Sheeta，Snigdha b.Ushnal，Laghu，Snigdh a c.Ushnal，Laghu，Snigdh a d.Ushnal，Laghu，Ruksha e.Sheetal，Laghu，Ruksh a f.Sheetal，Laghu，Ruksh a	a.Madhura b.Amla c.Madhura d.Katu e.Katu f.Katu

The character that contacts these materials and disease is selected medicine-criterion of therapeutical effect

Table 3: show and close the table that traditional Chinese medical science macrocosm is divided

SL NO.	Element	Season	Color	Taste	Rear impact	Development
								1	Wood	Spring	Blue	Acid	Wind	Birth
2	Fire	Summer	Red	Bitter	Heat	Growth
								3	Soil	Summer in evening	Yellow	Sweet	Tide	Grow
4	Gold	Autumn	In vain	Hot	Dry	Ripe
							5	Water	Winter	Black	Salty	Cold	Old and feeble

The basis that color is used for criterion of therapeutical effect

Table 4: show and close the table that traditional Chinese medical science macrocosm is divided

SL NO.	Element	Sense organ	Internal organs	Dirty	Feelings will	Structural outflow
							1	Wood	Eye	Bile	Liver	Angry	Sinus
2	Fire	Tongue	Small intestine	The heart	Happiness	Blood vessel
							3	Soil	Mouthful	Stomach	Spleen	Sorrow	Muscle
4	Gold	Nose	Large intestine	Lung	Sad	Hair
							5	Water	Ear	Bladder	Kidney	Probably	Bone

Table 5: show the contact of five kinds of native elements in the traditional Chinese medical science and the table of their relation

		Element			Element	Yin and Yang
							The 1st	Sky is brought up	Water	The 2nd	Forming energy	Relative with fire	The heart and small intestine
The 3rd	Sky is brought up	Wood	The 4th	Forming energy	With metallographic pair	Lung and large intestine
							The 5th	Sky is brought up	Soil	The 6th	Energy is finished	With water pair	Kidney and bladder
The 7th	Sky is finished	Fire	The 8th	Energy is finished	Relative with wood	Liver and gall-bladder
							The 9th	Sky is finished	Gold	The 10th	Energy is finished	With the soil phase pair	Spleen and stomach

Table 6: the table that shows Yin and Yang implication in the traditional Chinese medical science

Cloudy (dark side of hill)	Sun (plane of illumination of hill)
		Shallow	Deeply
Night	Daytime
		Wet	Do
Cold	Heat
		Water	Fire
Evil	Kindhearted
		Ugly	Beautiful
Crime	Virtue
		Poor	Rich
Sad	Happiness
		Chaotic	In order
Punishment	Reward
		Disease	Healthy
Passive	Actively
		The women	The male sex
Bad	Good
		Wife	The husband

Table 7: the table that shows the basis of carrying out the standardized color of curative effect of medication

Color	The white medicine	Yellow medicine	Red medicine	The black medicine
					The title of Sancrit	Shukla Varga	Peeta Varga	Rakta Varga	Krishna Varga
Sloka
					The title of medicine/material	Sudha Chuna (lime) Kachhapa Prista () tortoise shell Shankha (conch shell) Shukti (pearl shell) Varatika (little shell) Brushtashma (grey fossil) Sarkara (rock sugar) * Rajanighantu by Vaidya Narahari	Kusumba Pubshpa Kimshuka (single seed palas) Haridra (turmeric) Patanga (bush) Madayantika (Lasonia Inermis) * Rasamava	Dadima (pomegranate) Palasha (single seed palas) Laksha (Laccifera lacca) Bandhuka Haridra (turmeric) Kusumba Pushpa Manjista (madder) * Rajanighantu	Kadali (plantain) Karavellika (balsam pear) Triiphala (myribalans) Neelika (wood is blue) Nata (lemongrass) Panka (lotus flower) Kaseesa (Fe2S3) Balamra (prematurity mango) * Rasendra Chudamani

This medicine be named as sloka

Table 8: different colours is to the effect of various disease

The color effect			Natural	Heat production
							Violet	Indigo	Blue	Green	Yellow	Orange	Red
1. narrow 2. tumours of bone and bone 3. baldnesses 4. cataract 5. are blind	1.E-N-T problem 2. facial paralysis 3. lung diseases 4. asthma 5.T.B. 6. digestion power less thaies 7. nervous system problems 8. exhausting 9. amentias	Hundred cough throat problem fever typhoid fever smallpox cysticercosis gastric ulcer cholera brain swelling neurologic problems in vain loses eye mental depression seminal fluid emission problem burn, nose is bleeding etc.	Low or hypertension skin problem cancer influenza syphilis eye pain of cardiac problems etc.	The unusual spleen of all digestion, liver problem diabetes leprosy etc.	The refreshing neural epileptics of long-term asthma bronchitis tracheae swelling gout swelling kidney essense etc.	The wounded or disabled blunt flu paralysis hickie joint fire T.B. of anaemia etc.

The effect of color and on the impact at different human body position utilizes these bases of color to select medicines, for example opens the plant of indigo flower and will treat the ENT problem

Table 9: the character of six kinds of tastes (Rasas in the Ayurveda) and effect thereof

Taste	Essential element	Effect to Dosha	Embodiment
								The diet project	Medicine
Sweet (Madhur)	Soil+water	Kapha ↑ Vata and Pitta ↓	Sugar, banana, male fruit, raisins, coconut, raw sugar	Glycerrhiza Glabra, Asparagus Racemoses, gold
					Acid (Amla)	Soil+fire	Pitta and Kapha ↑ Vata ↓	Tamarind. buttermilk, curdled milk, living mango	Embalika officinalis
Salty (Lavana)	Water+fire	Pitta and Kapha ↑ Vata ↓	Salt	Rock salt
					Hot (Katu)	Air+fire	Pitta and Vata ↑ Kapha ↓	The ginger of the processing of Asoefetida, pepper powder, pimiento, drying	Piper Longum
Bitter (Tikta)	Space+wind	Vata ↑ Pitta and Kapha ↓	Bitter cucurbit	Azadiracta indica、Swertia   chiratita、Tinospora   Cordifolia
					Puckery (Kashaya)	Wind+soil	Vata ↑ Pitta and Kapha ↓	Honey	Terminalia chebula, Treminalia BelIerica, pearl, coral

It is how relevant with the infringement of disease with medicine to the figure illustrates taste.

Table 10: color and with the table of the relation of wavelength

Wavelength Nm	Color (from white light, absorbing)	Observe the complementary colors tone * of (emission)
			＜380	UV	Yellowish green
380-435	Violet	Yellow
			435-480	Blue	Orange
480-490	Turquoise	Red
			490-560	Bluish-green	Purple
500-560	Green	Violet
			560-580	Yellowish green	Blue
580-595	Yellow	Turquoise
			595-650	Orange	Bluish-green
650-780	Red
			＞780	Near-infrared

* the component that has these colors will absorb at each given wavelength place

These materials and medicine will demonstrate according to the absorption of particular color its color, and the color that absorbs is from the color gamut of irradiation white light thereon. They are handed over after absorbing and show final color.

Table 11: the acid and effect of alkalescence in human body

Table 12: the comparison sheet of prior art

SL No.	The technology of reporting	Method	Advantage	Defective
					1	The open chromatography of TLC	Graphic printing		1. simple 2. few 3. running costs consuming time are low	1. compare 2. variable effects that not exclusively separate fuzzy separation 3. analysis conditions that cause inferior to the chromatography of sealing and cause graphic printing unreliable 4. need to obtain other expensive instrument such as the support of LC-MS, NMR and IR, otherwise data transformation
2	The open chromatography of HPLC	Graphic printing		1. simple 2. few 3. running costs consuming time are low							1. compare 2. variable effects that not exclusively separate fuzzy separation 3. analysis conditions that cause inferior to the chromatography of sealing and cause graphic printing unreliable 4. need to obtain other expensive instrument such as the support of LC-MS, NMR and IR, otherwise the high instrument cost of data transformation 5.
					3	HPLC sealing chromatography (being better than open chromatography)	1. in the chromatogram of certain wave strong point	1. better separate 2. and be convenient to change the polarity of mobile phase to elute all molecules of whole polarity scope	1. need to obtain other expensive instrument such as the support of LC-MS, NMR and IR, otherwise the high running cost of the high instrument cost of data transformation 2. 3.
4	The HPLC sealing chromatography (being better than open chromatography) of suggestion	1. chromatogram represents that all wave-length coverages 2. adopt the profile chromatogram that organic and organic metal molecule are analyzed 3. and adopt the 3-D chromatogram that organic and organic metal molecule are analyzed	1. better separating 2. is convenient to change the polarity of mobile phase to elute all molecules of whole polarity scope. 3. be convenient to measure at the different wave length place of whole wave-length coverage 200-800nm the absorptance of molecule. It will can not stay any not in sight or not certified molecule. 4. be convenient to create the chromatographic fingerprinting of the domestic medicinal plant that WHO proposes ". 5. by conjugacy and the polarity of research by the component of this method separation, be convenient to understand the curative effect of this medicine. 6. (as the method for the identification of convict's individual character, it is used in the finger-print software by department of law court to be convenient to understand the curative effect of particular treatment group plant	1 high instrument cost (no better than or less than the HPTLC instrument) 2. high running cost the present invention is based on operational deficiencies 1. these computer based methods of computer method only to not having graduated outline-color spectrogram to carry out work. 2. adopt image software that the Image Adjusting size that produces working time and wave-length coverage 3. the images after analyzing that match to its X and the Y-axle can only be saved as the jpeg format that does not contain scale 4. that takies less Installed System Memory should adopt the coordinate system of expansion such as X1 and Y1 and store the image of shearing. But add more software features and can eliminate them.

The parameter that table 13 adopts the medicine finger-print

Figure number	The vegetable formal name of this plant	This name of the country	Use the position	The 3-D parameter
						Lifting height	The rotation height
				29	ABEL MOSCHUS MOSCHATUS     MEDICUM			KASTURI BENDA	Whole plant		20	15
30	ACACIA SUMA	SWETHAKHADIRA	Bark				15						65
				31	ACALYPHA INDICA			KUPINTA	Leaf		25	60
32	Malabar nut	VASA	Leaf				20						45
				33	ADIANTUM CAUDATUM			MAYURASHIKHI	Leaf		10	40
34	AILANTHUS EXCELSA	ARALU	The stem skin			10	65
				35	Calamus			VACHA	Rhizome		10	130
36	Leek	MAHALASUNA	LASSAN, large single-lobe cloves			20	130
				37	Garlic			LASUNA	LASSAN, large single-lobe cloves	20	130
38	Galanga Galangal Seed	GREATER     GALANGA.	Rhizome			20	75
				39	Galangal			LESSER GALANGA	Rhizome		15	75
40	ALPINIA SPECIOSA	LIGHTER     FALANGA	Rhizome				10						60
				41	Betel nut			BEETLE NUT	Unprocessed dry fruit	15	40
42	ARECA CATECHU	BEETLE NUT	The finished nut of milk			15	40
				43	ARECA KATEEH			RAKTHA     KHADIRA	The stem skin	15	65
44	ARNICA	ARNICA	Female tincture of whole plant			10	55
				45	Bacopa monnieri			BRAHMI	Herb		15	45
46	Dyer's barberry	DARUHARIDRA	Stem and bark			15	170
				47	BORRHIEVIA DIFFUSA			PUNARNAVA	Whole plant		15	55
48	Capsicum	MIRCH	Large ripening fruits			10	70
				49	Capsicum			MIRCH	Large ripening fruits	10	70
50	CA PSC ICUM ANNUML	MIRCH	Little immature fruit			10	70
				51	COSCINIUM DENESTRATIUM			LATA DARVI	The stem skin	15	125
52	Ivy gourd	DONDA	Root and leaf			25	30
				53	DACTLYLACTINIUM     AEGYPTIUM(ERECT)			GRASS	Leaf		25	40
54	DACTLYLACTINIUM     AEGYPTIUM(PROSTRATE)	GRASS	Leaf				25						40
				55	DIRISTACHIS CINERARIA			TUMMA	Leaf and bark	20	15
56	EMBLICA OFFICINALIS	ANALAKI	The fruit exocarp			5	50
				57	FACE PACK			BRAND1	Preparation		20	25
58	FACE PACK	BRAND2	Preparation				20						25
				59	Radix Glycyrrhizae			YASHTI MADHU	The root skin	15	130
60	Radix Glycyrrhizae	YASHTI MADHU	The whole plant powder			15	130
				61	GYMNEMA SYLVESTRAE			PODAPATRI	Whole plant		25	15
62	HOLLERONA ANTIDYSENTRICA	KUTAJA	The stem skin			10	60

63	Total shape elecampane	PUSHKARAMULA	Root	5	45
						64	Huang Lan	MANU SAMPENGA	Flower	20	40
65	Moringa	MUNAGA	Leaf	25	40
						66	Candleberry	BAY BERRY	The female tincture of cis therapy	20	35
67	NAHI AXILLAE	NAHI	Whole plant	10	130
						68	Oroxylum indicum	SYONAKA	The stem skin	10	170
69	Holy basil	RAMA TULASI	Leaf	15	130
						70	PLUCHEA LANCEOLATA	PATRA RASNA	Leaf	10	65
71	Storehouse network Radix picrorrhizae	KATUKI ROHINI	The stem skin	15	125
						72	Betel	BEETLE	Leaf	25	160
73	Psoralea corylifolia	BAKUCHI	Seed	25	60
						74	Blue beggar	MULILANGI，WHITE	Leaf	15	25
75	Castor-oil plant	ERANDA MULA	Root	10	135
						76	Madder	MANJISTA	Stem and root	10	40
77	The burdock hieracioides	KUSHTA	Root	5	80
						78	SPHERANTHUS INDICUS	MUNDI	Herb	15	70
79	SYMPLOCUS RACEMOSUS	LODHRA	The stem skin	15	65
						80	The myrobalan	HARITAKI	Fruit	10	40
81	TERMINALIA BELLERICA	VIBHITAKI	Fruit	20	35
						82	TRIGONELLA FAENUMG	MENTHI	Whole plant	15	160
83	Puncture vine	GOSHURA	Stem and root	25	45
						84	TYLOPHORA ASTHMATICA		Leaf	10	65
85	The pod fan	MOTHER TINCTURE	Female tincture of congeneric drug	20	15
						86	WITHINIA SOMNIFERA	ASWAGANDHA	Root	5	50
87	ZINZIBER OFFICINALIS	SHUNTI	The rhizome of the ginger of processing	15	130
						88	AVIPATTAKARA CHURNA	AYURVEDA     FORMULATION	Powder	25	60
89	KAMADUGA	SIDDHA     FORMULAT ION	Powder	10	25
						90	KUMARAYASAVA	AYURVEDIC     MEDDDICINE BY     FERMENTATION PROCESS	Liquid	10	35
91	MAHALAKSHMI VILAS RAS	SIDDHA     FORMULAT ION	Powder	20	35
						92	SUVARNA YOGARAJA GUGGULU	SIDDHA     FORMULATION	Powder	10	40

All other all be presented on separately the image about parameters such as wave-length coverage, trap and retention times.

Table 14: the medicine that carries out finger-print

	The vegetable formal name of plant	This name of the country	Effect *	Dosha Hara			Use the position
												PITTA	KAPHA	VATA
PITTA HARA ↓: the expression disease alleviate ↑: the expression disease increase the weight of
									AVIPATTAKARA CHURNA	AYURVEDA     FORMULATION	Loosen the gastric ulcer hemorrhoid	↓			Powder
	ACALIPHA INDICA	HARITA     MANJARI	Liver protection disease of skin women's diseases	↓			Leaf
									ANANDA BHAIRAVI	HERBOMINERAL	Pitta jwara	↓			Preparation
	AROGYA VARDHINI	HERBOMINERAL	The liver disease disease of skin	↓			Preparation
									BHUMYAMALAKI	PHYLILANTHUS     URINARIA	Jaundice	↓			Herb
	KAMADUGA	FORMULATION	Gastric ulcer	↓			Preparation
									KUMARAYASAVA	PERMENTATION PROCESS	Gyenic disease jaundice	↓			Liquid
	SARACA INDICA	HERBOMINERAL	The Gyeneac disease	↓			The stem skin
									SURYAVARTI		Headache	↓			Preparation

(the 2nd page in table 14)

KAPHA                  HARA
									AILANTHUS EXCELSA	ARALU	Disease of digestive system		↓
	ASPARAGUS ADESCENDENTUM	SAFED MUSALI	Medea, positive fistula		↓		Root
									Malabar nut	VASA	Respiratory disease		↓		Root
	ADIANTUM CAUDATUM	MAYURASHIKHI	Hemorrhoid, cough, diarrhoea		↓		Whole plant
									Garlic	LASUNA	Swasa		↓		Clove
	Leek	MAHALASUNA	Swasa		↓		Large single bulb
									ACACIA SUMA	SWETHA	Diabetes		↓		The stem skin
	Capsicum	KATUVEERA	Digestive disorders		↓		Large underdone fruit
									Ivy gourd	BIMBI	Emitic		↓
	CHOPACHINYADI CHURNAM	Herbal medicinal product	Venereal disease, skin disease		↓		Pulvis
									GLYCERRHZIA GLABRA	YASHTI MADHU	Panduroga，Rasay na		↓		Root, stem
	Ambrette	LATA KASTURI	Slheshma roga, Prameha, uropoiesis bladder and kidney disease		↓		The whole strain plant of wounded in the battle and seed
									INNULA RECEMOSA	PUSHKARAMULA	Kasa, swasa, jaundice, diabetes		↓		Root
	KRIMIKUTARA RAS	HERBO-MINERAL	Insect infection		↓		Preparation
									Holy basil	RAMA TULASI	Cough, fever		↓		Leaf

(the 3rd page in table 14)

	Blue beggar	MULKAWHITE	Diabetes, cough,		↓		Leaf
									SAUSSREA LAPPA	KUSHTA	Respiratory disease		↓		Root
	SHILAJIT(H)	HERBOMINERA L	Diabetes, kidney stone		↓		Pitch
									SHILAJIT(G)	HERBOMINERA L	Diabetes, kidney stone		↓		Pitch
	TY LOPHORA ASTHAMATICA	AJADWESHI	Asthama				Leaf
								VATA                HARA
	Galanga Galangal Seed	GREATER GALANGA	Rheumatic disease		↓		Rhizome
									Galangal	LESSER GALANGA	Rheumatic disease		↓		Rhizome
	ALPINIA SPECIOSA	LIGHTER GALANGA	Rheumatic disease		↓		Rhizome
									BRIHATVATACHINTAMANI+SV  RNAMAKSHKAM	HERBO-MINER AL	Arthritis		↓		Preparation
	BORRHIEVIA DIFFUSA	PUNARNAVA	Odema, urethra diuresis disease		↓		Whole strain plant
									HUTHASANA	HERBO-MINER AL	Various types of fevers		↓		Preparation
	MANHAYOGARAJA GUGGULU	HERBAL	Arthritis		↓		Preparation
									PLUCHEA LANCEOLATA	PATRA RASNA	Rheumatic disease		↓		Leaf

(the 4th page in table 14)

	Castor-oil plant	ERANDA MULA	Constipation, atrophic diseases			↓	Root
									SUVARNA YOGARAJA GUGGULU	The SIDDHA preparation	Rheumatic disease			↓	Pulvis
	SHITAMSU RAS	HERBO-MINER   AL	Jwara			↓	Preparation
									SUVAENA YOGARAJA GUGGULU	The HERBAL preparation	Arthritis			↓	Preparation
	VATA GAJANKUSA RAS	HERBO-MINER   AL	Sciatica			↓	Preparation
								PITTA          KAPHA          H     AR    A
	Catechu	RAKTHA   KHADIRA	Skin disease, diabetes	↓	↓		The stem skin, diffusate
									AILANTHUS EXCELSA	ARALU	Disease of digestive tract	↓	↓		The stem skin
	Betel nut	KRAMUKA	Diabetes, skin disease	↓	↓		The nut of milk processing
									AZARIDICTA INDICA	NIMBA	Skin disease, infectious diseases	↓	↓		Tender leaf
	Dyer's barberry	DARUHARIDRA	Fat, skin disease	↓	↓		The root skin
									CITRULLUS COLOSYNTHIS	INDRAVARUNI	Purgation, aborticide	↓	↓		HOMOEO MOTHER tincture
	Turmeric	TURMER IC	Insect infection, dysentery, diarrhoea, skin disease, wound	↓	↓		Commercial powder-1

(the 5th page in table 14)

	Turmeric	Turmeric	Insect infection, obesity, hemorrhage, skin disease, wound	↓	↓		Commercial powder-2
									Turmeric	Turmeric	Insect infection, dysentery, hemorrhage, skin disease, wound	↓	↓		Commercial powder-3
	COSCINIUM FENESTRATIUM	LATA DARVI	Diabetes, obesity, skin disease	↓	↓		Stem
									Turmeric	HARIDRA	Skin, allergy, diabetes	↓	↓		The rhizome of undressed mistake
	DACTYLACTIN IUM   AEGIPTIUM(PROSTRATEAND   ERECT)	GRASS	Diuretics, improve complexion	↓	↓		Whole strain leaves of plants
									EUGEN IA JAMBOLONA   SIZYGIUM CUMINI	JAMBU	Vomiting, diabetes, dysentery	↓	↓		Fruit
	HOLLERNAANTIDYSENTRICA	KUTAJA	Hemorrhage, class goes out blood sample	↓	↓		Stem skin from ANDHRA PREADES H
									HOLLERNAANTIDYSENTRICA	KUTAJA	Hemorrhage, all enterogastric diseases	↓	↓		Stem skin from KERALA
	Madder	MANJISTA	Skin disease, leukaemia, blood scarvenger	↓	↓		Stem, root
									PSORALIACORYLIF OLIA	BAKUCHI	White skin, skin disease	↓	↓		Seed, seed oil
	Storehouse network Radix picrorrhizae	KATUKA   ROHINI	Laxatives, hepatopathy	↓	↓		Root

(the 6th page in table 14)

	Hu Luba	METHIKA	Diabetes, colic	↓	↓		Whole strain plant
									The young tree of pearl	LODHRA	Hemorrhage, diarrhoea, hemorrhage, dysentery	↓	↓		Stem
	S PERANTHUS INDICUS	MUNDI	Krimihara, antimigraine, vrishya, lymphatic system disease	↓	↓		Whole strain plant
								KAPHA               VATA            HA     RA
	Calamus	VACHA	Medhya, language disease		↓	↓	Rhizome
									Aloe vera	KUMARI	Gynecological disease, hepatomegaly, splenomegaly, burn, uterine disease		↓	↓	Leaf, leaf juice
	AGN ITUNDINA	Herbal medicinal product	Indigestion	↑			Preparation
									Huang Lan	CHAMPAKA	Cosmetics, skin disease		↓	↓	Flower
	Capsicum	SIGRU	Abcess, oedema		↓	↓	Leaf
									Betel	NAGA VALLI	Kasa, swasa, digestive disease	↑	↓		From COASTAL ANDHRA PRADESH
	PIPER BEETLE	NAGA VALLI	Kasa, swasa, digestive disease	↑	↓		CULCATT A leaf
									TRIKATU CHURNA-1	Herbal medicinal product	Indigestion		↓	↓	Preparation
	TRIKATU CHURNA SP-2	Herbal medicinal product	Indigestion		↓	↓	Preparation

(the 7th page in table 14)

	TRIKATU CHURNA GH-3	Herbal medicinal product	Indigestion		↓	↓	Preparation
									Puncture vine	GOKSHURA	Urinary disorders, oedema		↓	↓	Stem and root
	TYLOPHORA ASTHMATIC	AJADWWAHI	Diabetes, asthma		↓	↓	Leaf
								PITTA           VATA                        HARA
	ACACIA SUMA	SWETHA   KHADIRA	Pramela	↓		↓	The stem skin
									ANANDABHAIRAVI	HERBO-MINER   AL		↓		↓	Preparation
	Bacopa monnieri	BRAHMI	Medhy, skin disease		↑		Whole strain plant
									DICROSTACHYS CINERA	VEERATARU   TUMMA	Hridya, obesity	↓		↓	Leaf and stem
	ENICOSTEMMA AXILLAE	NAHI	Malaria	↓		↓	Whole strain plant
									KANCHANARA GUGGULU	Herbal medicinal product	Inflammation	↓		↓	Preparation
	Oroxylum indicum	SYONAKA	Oedema, DD	↓		↓	The stem skin
								TRIDOSHA                  HARA
	ASPARAGUS ABSCENDENSES	SWETHA   MUSALI	Medea	↓	↓	↓	Root
									Capsicum	MIRCH，   KATIVEERA	Enterogastric diseases	↓	↓	↓	Large ripening fruits
	Thizoma curculiginis	KALI MUSALI	Medea	↓	↓	↓	Root

(the 9th page in table 14)

	EMBLICA OFFICINALIS	AMALAKI	Hridya，Rasaya，na   Neutraceutical	↓	↓	↓	Exocarp
									KARPURADI RAS	HAEMORRHOIDS	Diarrhoea	↓	↓	↓	Preparation
	MAHALAKSHMI VILAS RAS	HERBO-MINERAL	Various types of fevers		↓	↓	The z preparation
									ONOIN BIG	PALANDU	Hemorrhoid	↓	↓	↓	Bulb
	ONION SMALL	PALANDU	Hemorrhoid	↓	↓	↓	Bulb
									The myrobalan	HARITAKI	Laxatives, Rasayana	↓	↓	↓	Fruit
	TERMINALIA BELLERICA	VIBHITAKI	Kasa, swasa, skin disease, urethral calculus	↓	↓	↓	Fruit
									WITHINIA PUB.	The red seed of ASWAGANDHA, plant roots	General weak, rejuvenation effect	↓	↓	↓	Root
Imitation
									Face cream (G)		Effect is better				Preparation
	Face cream (B)		Do not contain some key components such as kushta (sausserea lappa) and manj ista (rubia cordifolia), so that the preparation effect descends				Preparation

(the 10th page in table 14)

	Chinese herbal medicine type shampoo powder (G)		Do not find foaming agent				Preparation
									Chinese herbal medicine type shampoo powder (B)		Mix with foaming agent				Preparation
Hahnemannian medicine
									ARNIC		Eliminate pain, effective to wound				Identical female tincture
	MYRICA CEREFERA	BAY BERRY	Protect liver				Identical female tincture trade mark-1
									Candleberry	BAY BERRY	Protect liver				Identical female tincture trade mark-2
	The pod fan	Female tincture	Gynecological disease				Same medicine
								The compound that separates
	Five-leaved chaste tree	Single compound	Protect liver				A kind of flavones 7-hydroxyl quercetin
									AZAMALYCIN	Single compound					The single compound of separating
	A kind of herbal medicine for the treatment of diabetes		Prameha				Allopathic acid amides component with diuresis is mixed
									Dyer's barberry	The single standard compound that separates					The salty reference material of barberry (FLUKA)

* the curative effect of reporting is that other places have also been reported much take the obtainable information of author as main.

Table 15: the medicine name that miniature finger-print is shown

The sample title	Used part
		Allopathic medicine	Figure 109
The Vitamin-B compound	Trade mark
		Alginodia	Trade mark
Atenolol	Trade mark
		Bromochlorobenzene  azoles amine	Trade mark
Citerizine	Trade mark
		Furazolidone	Trade mark
Brufen-paracetamol	Trade mark
		Paracetamol	Trade mark
The herbal medicine cosmetic sample	Figure 110
		Face cream (effect is poor)	Trade mark
Face cream (effect is good)	Trade mark
		Shampoo powder (effect is poor)	Trade mark
Shampoo powder (effect is good)	Trade mark
		Herbal medicinal product	Figure 111
Agnitundina	Trade mark
		Anandabhairavi	Trade mark
Arogyavardhani	Trade mark
		Brihatvatachintamani+Swarnamakshakam	Trade mark
Chopachinyadi Churnam	Trade mark
		Commercial Turmeric board 1	Trade mark
Commercial Turmeric board 2	Trade mark
		Commercial Turmeric board 3	Trade mark
Huthasana	Trade mark
		Hahnemannian 9 medicines	Figure 112
Arnica	Female tincture
		Calendula	Female tincture
Colosynthis	Female tincture
		The compound that separates	Figure 113
7-hydroxyl quercetin	From five-leaved chaste tree, separate
		A kind of flavones	From five-leaved chaste tree, separate
Azamalycin	Reference material
		Dexamethasone	Reference material
Single medicine	Figure 114
		Alovera	Leaf
Acalypha Indica	Leaf
		Embalika officinalis	Fruit
Aralu	The stem skin
		Betel nut	Seed
The Aswagandha red fruit	Root
		The Aswagandha gingko is real	Root
Andhra pradesh beetle leaf	Leaf
		Culcutta beetle leaf	Leaf
The root of fangji	The stem skin
		Dactlylactenium aegyptium(Erect)	Leaf
Dactlylactenium aegyptium(Prostrate)	Leaf
		Daruharidra	The stem skin
Haridra	Unprocessed rhizome
		Kalajamun	Fruit
Onion	Bulb
		Kutaja	The stem skin
Raktakhadira	Reel
		Shilajit	Finished pitch source 1
Shilajit	Finished pitch source 2
		Brahmi	Leaf

Table 16: finger-print is divided into treatment region according to conjugation and polarity

According to the color of reporting, whole image is divided into 3 zones and is divided into 3 zones at the Y-axle at the X-axle. X-axis represents the polarity scale by mobile phase composition. The Y-axle represents the conjugation that caused by the UV-VIS absorption value. When molecular conjugation more, the wavelength that its absorbs is higher (800nm) also. Like this, being present in the effect that will play of component in each district all has been presented at each shown among this figure treatment region each curative effect will be provided. Can carry out quantitatively these components with the UV-VIS absorbent properties, the absorbent properties of these UV-VIS are exactly directly proportional with the amount of component.

Claims (37)

1. method of using the extract component in chromatogram finger print measuring and differential plant or animal, natural or synthetic source, the step that the method contains is:

I) extract organic or organic-metallic compound with appropriate solvent from plant or animal, natural or synthetic source;

Ii) with high pressure liquid chromatography HPLC resulting extract in the step (i) is separated with polarity based on pH;

Iii) be created in profile and the 3D chromatogram of the composition that elutes in the step (ii);

Iv) resulting 3D and outline-color spectrogram are changed into colored image, analyze each color of colored image with the coordinate system of describing all 3 dimension characteristics of this image by using embedded software newly developed;

V) demonstrate concentration along with the various components of time wash-out;

Vi) produce chromatogram on the basis of color analysis, this figure has peak along with the conjugate property of component in each retention time;

Vii) ultraviolet and the both visible electromagnetic radiation UV-VIS absorption characteristic by various components in the image identifies compound in the component of described wash-out;

Viii) according to polarity and conjugate property to differentiating, measure and classify according to polarity, middle polarity and the less or nonpolarity compound that elutes;

Ix) use X-axis do reservation time, Y-axis make wavelength, R generates bar line code as the blue pixel number to the peak of selecting as green pixel number and B as number, the G of red pixel;

X) produce finger-print and bar line code database and differentiate each compound in the extract.

2. according to the process of claim 1 wherein that the solvent of opposed polarity is based on the hydrophily of the component that exists in the study sample and hydrophobicity and comes for extracting, ethanol is used as the preparation drug extract or to its standardized solvent.

3. under different pH scopes, identical drug extract is generated finger-print according to the process of claim 1 wherein.

4. according to the process of claim 1 wherein that used HPLC technology is by adopting any commercially available HPLC instrument with photodiode array detector.

5. according to the method for claim 1, wherein said method adopts the standard analysis parameter to carry out, comprise for finger-print, chemistry and acology standardization, use alcohol extract, kept operating time 0-60 minute, with acetonitrile and have the mobile phase wash-out of the phosphate buffer of pH in the 5.5-7.5 scope, and ultraviolet and visible detection device with 200-800nm electromagnetic radiation scope.

6. according to the process of claim 1 wherein that used solvent in the step (iii) is selected from the buffer solution of non-water, organic and moisture, water or known pH, and select according to the polarity scope.

7. according to the process of claim 1 wherein that an outline-color spectrogram changes into colored image, it has comprised conjugacy and the polarity of the component of institute's drugs extract.

8. according to the process of claim 1 wherein the curative effect of estimating drug extract with the quality that is present in the component in particular polarity and the UV-VIS uptake zone.

9. according to the method for claim 1, wherein use the X-axis that is provided by software to do reservation time, Y-axis and make wavelength, R and as the coordinate of blue pixel number the peak selected or peak group or image are generated bar line code as green pixel number and B as red pixel number, G, like this so that product is suitable for industry.

One kind computer implemented for detection of with the method for the extract with pharmaceutical value that derives from plant or animal, natural or synthetic source of identifying to obtain as claimed in claim 1, comprising:

(a) comprise in the opened file folder BMP, JPEG, TIF, GIF different-format the chromatographic fingerprint image and with single pixel sensitiveness the different color that exists in the image is analyzed;

(b) with such form display pixel information: 1. the image that has the X and Y coordinates specification, wherein X between 0 and the minutes specification between, and Y is between 200 to 800nm, and 2. except this figure, the circular diagram of the independent numerical value at each peak in two kinds of splitters;

(c) use the PRINT icon to print and analyze rear all data that generate;

(d) use PAGE SETUP icon to change the page setup of printing;

(e) use RESIZE icon selection portion partial image and analysis;

(f) different images is opened the graphical analysis window of any number, and with WINDOW icon show state;

(g) use the ZONE icon at 20 minutes intervals image to be divided into three kinds of zones;

(h) use the INVERT icon to make the Image Reversal of selection;

(i) using the EDITOR icon to switch to known text writes/editing application software;

(j) use the demonstration of HELP icon about the operation information of the various features of this software; With

(k) use SAVE AS icon with according to^*.JEPG file format stores the data that produce.

11. in accordance with the method for claim 10, wherein extracting used solvent is that the polarity according to the component of institute's study sample, hydrophilic and hydrophobic property are selected.

12. in accordance with the method for claim 10, wherein the HPLC instrument is selected from any commercially available HPLC instrument with photodiode array detector.

13. according to the method for claim 10, wherein the polarity of mobile phase be the ratio from 0% to 100% by changing mobile phase known pH water-containing buffering liquid and comprise the nonaqueous solvents of acetonitrile or control on the contrary.

14. according to the method for claim 10, comprising analyzing 3D and outline-color spectrogram, obtaining at X and Y-axis is the chromatogram of retention time and wavelength.

15. according to the method for claim 11, obtain data with percentage quantificational expression " doshas infringement " comprising analyzing 3D and outline-color spectrogram.

16. according to the method for claim 11, comprising using single solvent alcohol extract component; All extraction components are produced treatment with identical analysis condition and instrument parameter unitize, realize like this standardization for the treatment of.

17. the method according to claim 1, it is chromatographic fingerprinting, chemistry and criterion of therapeutical effect and to the computer approach from the organic or organic-metallic molecule bar line encode of plant or animal, material that naturally obtain or that manually prepare as medicine, and the method comprises:

A) select to have plant, animal or the material that naturally obtain or artificial preparation of drug value, and extract its component;

B) component is separated into individualized compound, generates 3D and outline-color spectrogram and be converted into finger-print,

C) use this finger-print of software analysis of developing, and

D) decryption.

18. in accordance with the method for claim 1, wherein provide embedded software to be used for component that research extract is existed and conjugacy and polarity thereof in step (iv) and carried out chemical analysis, the new software that use is developed represents the curative effect of medicine according to traditional concept.

19. in accordance with the method for claim 1, embedded software provides the new ideas of the chromatographic fingerprinting of the material that obtains to have pharmaceutical value in the step (iv), and this new ideas can be used for differentiating fast and be present in the TP of the compound in the medicine that uses and the curative effect of these components.

20. in accordance with the method for claim 1, embedded software provides the new chromatographic fingerprinting of a kind of herbal medicine and preparation in step (iv), use the profile of the herbal medicine of analyzing and preparation and 3D chromatogram and its photodiode array detector PDA in high pressure liquid chromatography developed, this has described the spectral characteristic data of the component that is present in the material with drug value, and these data are that the particular order according to the polarity that generates under the similar test analysis condition presents.

21. in accordance with the method for claim 1, wherein " chromatographic fingerprinting " becomes the blueprint that is present in the component in herbal medicine or the preparation in step (vi), and to the quick discriminating of institute's drugs.

22. in accordance with the method for claim 1, wherein concerning finger-print and chemistry and treatment standardization, adopt identical standard analysis parameter, comprise with same solvent alcohol extract, 0-60 minute same operation time, identical mobile phase acetonitrile and have the phosphate buffer of pH in the 5.5-7.5 scope, and identical UV-visible range 200-800nm.

23. in accordance with the method for claim 1, wherein in step (ix), pure and mild impure research is studied and differentiated to the sample with commercially available food and medicine that the finger-print data chromatogram of acquisition is used for mixing, replacement, dispute.

24. in accordance with the method for claim 1, wherein in step (ix), process standardization, quality control activity and treatment aims of standardization that the finger-print data chromatogram of acquisition is used for for allopathic, Ayurveda, hahnemannian, siddha, unani, Chinese medicine, Tibetan medicine, kampo medicine are differentiated the chemical composition that is present in wherein.

25. in accordance with the method for claim 1, wherein in step (ix), the finger-print data chromatogram that obtains is used for research at naturally occurring sample because the variation of the chemical composition that different ecological factor, geographic factor, genotype and the phenotypic variation in plant cause, and discriminating wherein chemical composition and they are carried out standardization.

26. in accordance with the method for claim 1, wherein in step (ix), the finger-print data chromatogram that obtains is for the research of the chemical composition of the sample of involutory one-tenth preparation, and differentiate chemical composition wherein and be chemistry and treat standardization, whichsoever applicable, chemical composition is carried out standardization.

27. in accordance with the method for claim 1, wherein in step (ix), the finger-print data chromatogram of acquisition be used for the single medicine sample the herbal products chemical composition research and differentiate wherein chemical composition for chemistry and criterion of therapeutical effect.

28. in accordance with the method for claim 1, wherein in step (ix), the finger-print data chromatogram of acquisition is differentiated chemical composition wherein for the research of the chemical composition of the herbal medicine product of patent medicine sample with for the standardization of chemistry and curative effect.

29. in accordance with the method for claim 1, wherein in step (ix), the finger-print data chromatogram of acquisition is used for the research of variation of chemical composition of biological sample and discriminating and standardization chemical composition wherein and is used for chemistry and acology standardization.

30. in accordance with the method for claim 1, wherein in step (ix), the finger-print data chromatogram of acquisition be used for the single of different brands and the food of making and drug sample product chemical composition variation research and differentiate wherein chemical composition for the standardization of chemistry and curative effect.

31. in accordance with the method for claim 1, wherein the preparation of the database of a large amount of samples has provided the many summaries to the curative effect of particular group plant, and this group classification is according to disease specific or treatment classification.

32. in accordance with the method for claim 1, the finger-print data of its Chinese traditional medicine so that according to from the polarity of 3D and outline-color spectrogram and conjugacy to the classification of drug ingedient and quantitatively easier and can estimate the curative effect of medication that will act on the body fluid.

33. in accordance with the method for claim 1, wherein use the conjugacy and the polarity that provide among the chromatographic fingerprint figure, the finger-print data that obtain make it possible to understand the physical-chemical characteristic that comprises color with the standardization medicine, are used for the treatment standardization to medicine and body fluid.

34. in accordance with the method for claim 1, wherein use conjugacy shown in the chromatographic fingerprinting and polarity, the finger-print data that obtain make it possible to understand the physical-chemical characteristic that comprises taste with the standardization medicine, this taste comprises acid, salty, hot, bitter, puckery, thereby is used for making the treatment standardization.

35. in accordance with the method for claim 1, wherein use conjugacy and the polarity of the one-component shown in the chromatographic fingerprinting and whole medicine, the finger-print data that obtain make it possible to understand the physical-chemical characteristic with the standardization medicine, comprise character, potentiality, metabolin, comprise the property of the chirality of molecule, be used for the treatment of standardization.

36. in accordance with the method for claim 1, wherein use conjugacy and the polarity of the drug extract shown in the chromatographic fingerprinting, the finger-print data that obtain make it possible to understand the physical-chemical characteristic with the standardization medicine, comprise hot and cold, effect slowly, effect violent, heavy, light, soft lubricated bending, drying, be used for the treatment of standardization.

37. in accordance with the method for claim 10 the 3D chromatogram of process element and the computer based data processor of color contour images comprise processor, described processor is arranged to:

I) analyze coloured contour images based on the shades of colour of selecting, wherein shades of colour represents the in time concentration of the various components of wash-out, and the polarity that is as the criterion with retention time;

Ii) the 3D chromatogram of all 3 dimension specificity analysis drug extracts of use image;

Iii) generate the chromatogram with peak, these peaks occur in the conjugacy that different retention times is accompanied by in time the compound that the particular order with polarity elutes;

Iv) ultraviolet by various elution fractions in the image and both visible electromagnetic radiation UV-VIS absorption characteristic are differentiated the compound in this extract;

V) with the biology of the various elution fractions that exist in the pharmaceutical samples of studying, therapeutic activity by finger-print being divided into the treatment region on X and Y-axis and be associated based on polarity and the conjugacy of molecule;

Vi) by the X-axis of utilizing described method to provide do reservation time, Y-axis do wavelength, R as red pixel numerical value, G as green pixel numerical value, the B image coordinate system as blue pixel numerical value, generate the bar line code at selected peak;

Vii) generate the finger-print of sample and the database of bar line code, so that the database application of all kinds such as enterprise resource planning ERP and user's contact management CRM application are easier; With

Viii) generate the database of " display window " of the used all samples of the business applications of enterprise resource planning ERP and user's contact management CRM type.

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