CN100346797C - Compound injection from ice tablet and ginkgo leaf extract and its making method thereof - Google Patents
Compound injection from ice tablet and ginkgo leaf extract and its making method thereof Download PDFInfo
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- CN100346797C CN100346797C CNB2004100653358A CN200410065335A CN100346797C CN 100346797 C CN100346797 C CN 100346797C CN B2004100653358 A CNB2004100653358 A CN B2004100653358A CN 200410065335 A CN200410065335 A CN 200410065335A CN 100346797 C CN100346797 C CN 100346797C
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- borneolum syntheticum
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Abstract
The present invention relates to compound injection preparation which contains effective parts of traditional Chinese medicine for treating angiocarpy and cerebral vessel diseases, and a preparation method. One part of borneol which is traditional Chinese medicine by weight and 2 to 20 parts of gingko leaf extract by weight are combined according to a compounding ratio, which prepares the compound preparation, and the compound injection preparation is also prepared. The compound injection preparation of the present invention enhances the pharmacological action and the effect time of traditional oral medicine. Compared with a mode that one of borneol and gingko leaf extract is singly used, the medicine effect is obviously enhanced, and attenuation of virulence is obtained. The compound injection preparation is used for treating cerebral infarction, cerebral ischemia, coronary heart diseases, angina pectoris, myocardial infarction, etc. The compound injection preparation has the characteristics of quick effect, obvious effect, high safety and high stability.
Description
Technical field
The present invention relates to a kind of compound injection of forming by a certain percentage by Borneolum Syntheticum and Folium Ginkgo extract and preparation method thereof.Belong to medical technical field.
Background technology
Cardiovascular and cerebrovascular disease is first killer of harm humans life and health, according to updated statistics, the whole world had 1,700 ten thousand people to die from cardiovascular disease in 2000, account for 1/3 of the total death toll of global a variety of causes, expect this numeral of the year two thousand twenty and will increase to 2,500 ten thousand, and wherein 80% all in developing country.
According to China's population analysis on cause of death in 1997, tumor held pride of place in the urban population cause of death, and cerebrovascular accounts for second, and both numerical value is bordering on equal; Respiratory system disease holds pride of place in the rural population cause of death, and cerebrovascular accounts for second.Ischemic cerebrovascular is all accounting for 70% in the cerebrovascular.Cardiovascular and cerebrovascular disease has quite high sickness rate and disability rate, can not be ignored.
Semen Ginkgo belongs to a kind of kind for only depositing a section one, have the title of gymnosperm " living fossil ".Mainly contain: blood circulation promoting, prevention cardiovascular and cerebrovascular disease, lifting memory, functions such as prevention senile dementia, antioxidation, the formation of prevention blood clot.At present, from Folium Ginkgo, isolated 30 activity chemistry compositions, but clinical medicinal main chemism composition still is total flavones and total lactone.Gingko leaf preparation is mainly used in treatment cardiovascular and cerebrovascular disease, neural mental disease, diseases of the five sense organs, respiratory system disease, urologic disease clinically with other cancer, to the protective effect of organ transplant rejection etc., and Semen Ginkgo is very low in mammiferous toxicity.
Borneolum Syntheticum has the refreshment of having one's ideas straightened out, the effect of clearing away heat to alleviate pain.Modern study shows, Borneolum Syntheticum not only self very permeable is crossed blood brain barrier, and the effect of performance consciousness-restoring and orifice-opening can also increase the blood-brain barrier permeability of other medicines, increases the curative effect of medicine to brain diseases.
Its distribution half-life of Borneolum Syntheticum intravenously administrable is 2.8 minutes, be distributed to histoorgan performance curative effect very soon, and the oral elimination half-life is 5.3 hours, illustrates that Borneolum Syntheticum absorbs slowly and bioavailability is low.Borneolum Syntheticum amount in minority injection such as XINGNAOJING ZHUSHEYE, QINKAILING ZHUSHEYE is also very low, is difficult to reach medicine effective concentration and affects the treatment.
Do not find as yet with the compound preparation of Folium Ginkgo extract and Borneolum Syntheticum compatibility separately, its correlational study document does not see that report is arranged yet, both have good therapeutical effect to the cardiovascular and cerebrovascular vessel acute illness, can bring into play synergism behind the compound recipe, cardiovascular and cerebrovascular vessel acute attack stage particularly, onset and reach desired therapeutic effect rapidly.
Summary of the invention
The object of the present invention is to provide a kind of compound injection of being made up of by a certain percentage Chinese medicine Borneolum Syntheticum and Folium Ginkgo extract, can be used for cardiovascular and cerebrovascular disease, it can overcome the shortcoming that the oral formulations absorption difference is waited a moment.Pharmacodynamic experiment proves that the Chinese medicine of forming with the effective site Folium Ginkgo extract and the Borneolum Syntheticum of Folium Ginkgo extract is used for the qi depression to blood stasis cardiovascular and cerebrovascular disease, and is evident in efficacy.This compound injection agent medicine shows that through toxicological experiment toxicity is minimum, and through the side's of tearing open research, these two medicines of writing out a prescription lump together adapted, obviously strengthens with any not only drug effect wherein than single, and has Attenuation.The present invention also provides the preparation method of above-mentioned compound injection.
The effective ingredient in Chinese compound injection that the present invention proposes, the compound injection of forming by a certain percentage by Chinese medicine Borneolum Syntheticum and Folium Ginkgo extract.
This effective ingredient in Chinese compound injection, its proper proportioning is: Borneolum Syntheticum: 1 part, Folium Ginkgo extract: 2-20 part.
This compound injection can be dosage forms such as liquid drugs injection, powder pin and transfusion.
This compound injection is in order to various cardiovascular and cerebrovascular diseases such as treatment ischemic brain injury, angina pectoris, myocardial ischemia.
The compatibility of this compound injection uses, major advantage is: Borneolum Syntheticum can increase the effective ingredient bioavailability in vivo of Semen Ginkgo, improve its curative effect, strengthen central action, Semen Ginkgo can be improved the dissolubility of Borneolum Syntheticum in water, Folium Ginkgo extract and the potentiation of Borneolum Syntheticum compatibility, this preparation is rapid-action, the curative effect height.
The preparation of Folium Ginkgo extract can be undertaken by following technology, the Folium Ginkgo of getting certain mass is with the finite concentration alcohol reflux, concentrating under reduced pressure, after the clarifier sedimentation, concentrating under reduced pressure is with the 50% left and right sides ethanol dried coarse powder of desilver Folium Pruni that boils again, adsorption resin column on the filtrate, at last pale yellow powder shape extract, contain flavonoid glycoside and Ginkgo total lactones and be not less than 24%, 6% respectively.
Borneolum Syntheticum is the commercially available prepared slices of Chinese crude drugs that meet the Chinese Pharmacopoeia standard.
The invention allows for above-mentioned composition, its liquid drugs injection, transfusion, powder injection formulation prepare as follows:
Get Borneolum Syntheticum 1 weight portion, fine ground, add 30-70 weight portion glycerol heated and stirred and make dissolving fully, standby.Get Folium Ginkgo extract 2-20 weight portion, add an amount of water for injection dissolving, the glycerite of above-mentioned Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, stir, the osmotic pressure regulator of making milliosmolaritys such as need adding to when infusing makes dissolving, this osmotic pressure regulator is at least a in mannitol, sodium chloride or the glucose, is diluted to certain volume with proper amount of water for injection, mixing.Medicinal liquid is the ultrafilter membrane ultrafiltration of 10000～30000 molecular weight through flux, and embedding becomes injection or transfusion, and sterilization promptly gets injection, primary infusion preparation.
Get Borneolum Syntheticum 1 weight portion, fine ground, add an amount of anhydrous alcohol solution after, slowly add with 8-15 weight portion HP-and be made in the saturated aqueous solution, be incubated 40-60 ℃, stir or ultrasonicly make the solution clarification.Other gets Folium Ginkgo extract 2-20 weight portion, adds an amount of water for injection dissolving.The HP-enclose solution of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, stir, the osmotic pressure regulator of making milliosmolaritys such as need adding to when infusing makes dissolving, this osmotic pressure regulator is at least a in mannitol, sodium chloride or the glucose, be diluted to certain volume with proper amount of water for injection, mixing.The ultrafilter membrane ultrafiltration of medicinal liquid via hole diameter 30000～100000 molecular weight, embedding becomes injection or primary infusion, and sterilization promptly gets injection, primary infusion preparation.
Get Borneolum Syntheticum 1 weight portion, fine ground, after adding an amount of anhydrous alcohol solution, slowly add with 8-15 weight portion HP-and be made in the saturated aqueous solution, be incubated 40-60 ℃, stirring or the ultrasonic solution that makes are clarified, other gets Folium Ginkgo extract 2-20 weight portion, adds the dissolving of an amount of water for injection, and the HP-enclose solution of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, stir, add an amount of forming agent again and make dissolving, this forming agent is a mannitol, at least a in HP-or the glucose is diluted to certain volume with proper amount of water for injection, stir, the ultrafilter membrane ultrafiltration of medicinal liquid via hole diameter 30000～100000 molecular weight, ultrafiltrate packing, lyophilizing, gland promptly gets the powder pin.
Pharmacodynamic experiment of the present invention
One, to the effect of mice ischemia-reperfusion learning memory disorder model
Get 6 groups of male SD mices and be divided into sham operated rats, model group, TANSHINONES positive drug control group and compound recipe silver ice injection group, Folium Ginkgo extract group, Borneolum Syntheticum group, every group of 10 animals at random, irritate stomach every day 1 time, blank group and model group such as give at the capacity normal saline, continuous 15 days, 1h after the last administration caused the imperfection bilateral bloodstream blocking of mice according to method, cause cerebrum ischemia, behind the 5min, remove vascular ligation, make common carotid artery reply blood flow and pour into again.Use the multi-functional avoidance condition of JZZ94 instrument next day, carry out the passive learning memory training, errors number (N), stimulation time (Ts) and platform retention (T) that the record mice jumps off electric grid from diving tower with the diving tower method.Test again after 24 hours.The results are shown in Table 1.
The influence (X ± S n=10) of table 1 pair mouse brain ischemia-reperfusion learning and memory of little mouse
| Group | Dosage every day (mg/kg) | Plateau (s) | Stimulation time (s) | Mistake number (N) |
| Blank model group silver ice group (1: 1) silver-colored ice group (1: 5) silver-colored ice group (1: 10) ginkgo group borneol group tanshinone group | 10 10 10 8 2 15 | 296.1±2.0 290.2±5.4 ## 296.8±2.1 ** 297.7±2.2 ** 295.6±2.2 ** 294.3±1.6 * 290.3±3.1 294.1±1.8 * | 0.4±0.66 4.3±2.6 ## 1.1±0.5 ** 1.4±0.7 ** 0.9±0.7 ** 2.1±1.1 * 4.2±2.4 1.8±0.5 ** | 1.8±1.8 3.5±1.9 0.8±0.7 ** 0.7±0.6 ** 1.3±1.1 * 2.5±1.5 3.0±1.7 1.6±2.0 |
Annotate: compare with the blank group,
##P＜0.01; Compare with model group,
*P＜0.05,
*P＜0.01.
Result of the test shows that model group and sham operated rats relatively have significant difference (P＜0.01), shows mice modeling success.Compound recipe silver ice injection and model group relatively have significant difference (P＜0.01), show that this injection increases significantly to the memory ability of mice.Compare with the Folium Ginkgo extract group (P＜0.05), the compound injection group has better effect to the memory of mice, and the Borneolum Syntheticum in the compound recipe sees through blood brain barrier at it, promotes drug absorption, promoting the medicine bulk concentration to increase the aspect has better action, has also shown the reasonability of this compound recipe.
Two, the influence of the persistent period of panting behind the mice broken end is tested
Get 50 of Bal/BC mices, be divided into blank group, nimodipine positive drug control group and silver ice injection use compound group, Borneolum Syntheticum group, Folium Ginkgo extract group at random, every group of 10 animals.Wherein, silver ice dosage is according to document conversion animals administer dosage, and the nimodipine group is according to human body consumption conversion mice dosage.Blank group normal saline is irritated stomach, and all the other are respectively organized relative medicine and irritate stomach, and 1 time/d, continuous 7d.15min after the last administration breaks end under the mice basal part of the ear fast, and record begins to pant the persistent period as broken end to its time that stops of panting for the last time from broken end.The results are shown in Table 2.
Influence (the n=10 of X ± S) of persistent period pants behind the table 2 pair mice broken end
| Group | Dosage every day (mg/kg) | (s) breathes the persistent period |
| Blank group silver ice group Semen Ginkgo group Borneolum Syntheticum group nimodipine group | l0 8 2 10 | 20.1±1.37 24.5±1.52 ** 20.35±1.43 22.7±1.28 * 23.45±1.69 |
Annotate: compare with the blank group,
*P＜0.05,
*P＜0.01.
Experimental result shows, ig compound recipe silver ice, single Borneolum Syntheticum are panted the persistent period after all obviously prolonging the mice broken end, with the blank group relatively, be significant difference (
*P＜0.05,
*P＜0.01), it is not obvious that ig single Folium Ginkgo extract prolongs the persistent period of panting after mice breaks end, and compares there was no significant difference with the blank group.But compound recipe silver ice injection group is more more remarkable than the effect that Borneolum Syntheticum group and Folium Ginkgo extract group prolong the persistent period of panting behind the mice broken end, and the prompting compound injection can significantly improve cerebral ischemia treatment of diseases effect, and is more more superior than the therapeutic effect of single medicine.
Three, intraluminal middle cerebral artery occlusion in rats is caused the effect of cerebral ischaemia
Get 6 groups of male SD rats and be divided into sham operated rats, cerebral ischemic model group, nimodipine positive drug control group and compound recipe silver ice injection group, Folium Ginkgo extract group, Borneolum Syntheticum group, every group of 10 animals at random.Cause cortex of cerebral hemispheres and the basal nuclei ischemia injury model of rat according to blocking-up rat medium-sized artery (MCAO) method.Each organizes intravenous injection once, and administration is after 30 minutes, and each Mus is with 10% chloral hydrate anesthesia (300mg/ (kg, ip), undergo surgery by literature method, postoperative 24 hours broken end is got brain, dyed after, take out respectively and be normal cerebral tissue rosy and be linen blocking tissue, weigh,, obtain the percentage ratio that the infarct area accounts for the hemisphere gross area then according to the weight area method, t method of inspection and model group are carried out significance test relatively between i.e. infraction rate, and employing group.The results are shown in Table 3.
The influence of the table 3 pair rat cerebral infarction scope (n=10 of X ± S)
| Group | Dosage (mg/kg) | Number of animals (n) | Infarct size/brain sections the gross area (%, X ± S) |
| Sham operated rats model group silver ice group Semen Ginkgo group Borneolum Syntheticum group nimodipine group | 10 8 2 2 | 10 10 10 10 10 10 | 0 11.5±4.1 ## 8.1±4.0 ** 8.9±4.4 * 10.6±5.7 8.0±4.1 * |
Annotate: compare with sham operated rats,
##P＜0.01; Compare with model group,
*P＜0.05,
*P＜0.01.
Result of the test is learned by statistics and is shown, model group has been compared significant difference with sham operated rats, shows rat MCAO modeling success.Compare with model group; compound recipe silver ice injection group, Folium Ginkgo extract group all can reduce animal brain cerebral infarction rate; but compound recipe silver ice injection group has been compared significant difference (P＜0.01) with it; its protective effect to middle cerebral artery blocking-up (MCAO) rat is better than Semen Ginkgo group and nimodipine group (P＜0.05), shows the superiority of silver ice compound recipe thus.
Four, to the effect of rat acute liver injury model
Extracting male Wistar rat is divided at random: matched group, model group, basic, normal, high three the dosage groups of silver ice preparation, Folium Ginkgo extract group, Borneolum Syntheticum group and oleanolic acid positive drug control group, 10 every group.D-Gal amine (D-GalN) causes the rat acute liver injury model, give curative drug silver ice injection, Semen Ginkgo and positive drug placebo (oleanolic acid), 1 time/d, be total to 8d, the 7th day morning, give D-GalN modeling, the 8th day, broken end was got blood behind the last administration 1h, measured rat blood serum platelet activating factor (PAF), alanine aminotransferase (ALT), malonaldehyde (MDA), and light microscopic observation hepatic pathology variation down, the results are shown in Table 4.Table 5.
Table 4 is respectively organized the situation of change of rat blood serum PAF, ALT, MDA
| Group | Dosage (mg/kg) | PAF(μg/L) | ALT(U/L) | MDA(nmol/L) |
| Normal group model group silver ice group (1: 1) silver-colored ice group (1: 5) silver-colored ice group (1: 10) ginkgo group borneol group oleanolic acid group | 10 10 10 8 2 60 | 1.09±0.18 4.6±0.33 ## 2.74±0.28 ** 2.65±0.22 ** 2.03±0.20 ** 3.12±0.27 ** 4.58±0.33 4.53±0.45 ** | 55.8±6.38 1092.7±88.50 ## 498±73.03 ** 501.7±45.04 ** 350.6±66.77 ** 521.3±60.1 ** 1089.8±86.3 508.8±58.91 ** | 5.86±2.70 16.43±3.78 ## 9.37±2.47 ** 8.62±2.33 ** 5.39±2.27 ** 7.47±2.41 ** 16.34±3.69 8.78±2.98 ** |
Annotate: compare with sham operated rats,
##P＜0.01; Compare with model group,
*P＜0.05,
*P＜0.01.
Result of the test shows that silver ice injection and Semen Ginkgo extrac group all have the good protective effect to acute liver damage, but compare with Semen Ginkgo, and the Borneolum Syntheticum in the silver ice has significant promotion to Semen Ginkgo extrac and absorbs, and improves bulk concentration, increases the superiority of curative effect.
The D-GalN that influences to pathological change brings out the rats'liver damage model, because of its pathological change is widely used to human virus's hepatitis is similar.This test pathological observation shows: hepatocyte bulk or inferior massive necrosis, and lobules of liver and hepatocyte disappear, and there were significant differences for hepatic pathology credit level and normal group (P＜0.01).Each dosage group blood-serum P AF of Semen Ginkgo, ALT, MDA and positive controls ALT, MDA then all are starkly lower than model group, hepatic necrosis obviously alleviates, and scope is dwindled, and visible lobules of liver structure, wherein preferable with silver ice dosage group curative effect especially, other each treatment group effect no significant differences.Conclusion: silver ice injection has protective effect to rat acute hepatic injury due to the D-GalN; its mechanism may be by removing oxygen-derived free radicals, antagonism PAF; improve that liver microcirculation realizes; Borneolum Syntheticum can significantly increase Semen Ginkgo effective ingredient concentration in vivo; improve its bioavailability, be expected to one of active drug that becomes the acute Severe Viral Hepatitis of clinical treatment.
Specific embodiment
Embodiment 1: the preparation of Folium Ginkgo extract
The Folium Ginkgo of getting certain mass is with 65% alcohol reflux, concentrating under reduced pressure, after the sedimentation of ZTC clarifier, concentrating under reduced pressure is with the 50% ethanol dried coarse powder of desilver Folium Pruni that boils again, last 724 adsorption resin columns of filtrate, at last pale yellow powder shape extract, contain flavonoid glycoside and Ginkgo total lactones is respectively 26%, 10.6%.
Embodiment 2: the preparation [] of silver ice injection
Get Borneolum Syntheticum (artificial or natural) 20g, fine ground, add 1000ml glycerol heated and stirred and make dissolving fully.Get Folium Ginkgo extract 2g and add the dissolving of 2-4L water for injection.The glycerite of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, and the limit edged stirs to clarify, and is diluted to 10L with water for injection.The medicinal liquid via hole diameter is the ultrafilter membrane ultrafiltration of 10000 molecular weight, embedding, and 10ml/ props up, and sterilization promptly gets injection.
Embodiment 3: the preparation [two] of silver ice injection
Get Borneolum Syntheticum (artificial or natural) 20g, fine ground, add an amount of anhydrous alcohol solution after, slowly add with the 600g HP-and be made in the saturated aqueous solution, be incubated 40-60 ℃, stir or ultrasonicly make the solution clarification.Other gets Folium Ginkgo extract 4g, adds an amount of water for injection dissolving.The HP-enclose solution of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, stir, the osmotic pressure regulator of making milliosmolaritys such as need adding to when infusing makes dissolving, this osmotic pressure regulator is at least a in mannitol, sodium chloride or the glucose, be diluted to 20L with water for injection, mixing.The ultrafilter membrane ultrafiltration of medicinal liquid via hole diameter 30000～100000 molecular weight, embedding, 10ml/ props up, and sterilization promptly gets injection.
Embodiment 4: the preparation [] of silver ice transfusion
Get Borneolum Syntheticum (artificial or natural) 20g, fine ground, add 2400ml glycerol heated and stirred and make dissolving fully.Get Folium Ginkgo extract 2g and add the dissolving of 2-4L water for injection.The glycerite of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, the limit edged stirs, and the osmotic pressure regulator of milliosmolarity such as adds to, and this isotonic agent is at least a in mannitol, sodium chloride or the glucose, and be diluted to 100L or 250L with water for injection, stir.The medicinal liquid via hole diameter is the ultrafilter membrane ultrafiltration of 30000 molecular weight, and it is an amount of to replenish water for injection, is distributed into 100ml or 250ml/ bottle, irritates lid, and sterilization promptly gets and infuses.
Embodiment 5: the preparation [two] of silver ice transfusion
Get Borneolum Syntheticum (artificial or natural) 20g, fine ground, add an amount of anhydrous alcohol solution after, slowly add with the 200g HP-and be made in the saturated aqueous solution, be incubated 40-60 ℃, stir or ultrasonicly make the solution clarification.Other gets Folium Ginkgo extract 4g, adds an amount of water for injection dissolving.The HP-enclose solution of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, stir, the osmotic pressure regulator of making milliosmolaritys such as need adding to when infusing makes dissolving, this osmotic pressure regulator is at least a in mannitol, sodium chloride or the glucose, and be diluted to 100L or 250L with water for injection, stir.The medicinal liquid via hole diameter is the ultrafilter membrane ultrafiltration of 30000 molecular weight, and it is an amount of to replenish water for injection, is distributed into 100ml or 250ml/ bottle, irritates lid, and sterilization promptly gets and infuses.
Embodiment 6: the preparation of silver-colored freezing dry powder injection
Get Borneolum Syntheticum (artificial or natural) 20g, fine ground, add the 40ml anhydrous alcohol solution, standby.Other gets HP-240g, is made into drink and solution, adds Borneolum Syntheticum ethanol liquid while stirring, and stirring or ultrasonic enclose get settled solution.Get Folium Ginkgo extract 4g, add an amount of water for injection dissolving.The hydroxypropyl enclose solution of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, and the limit edged stirs to clarify, and is diluted to 50L with water for injection, dissolving.Medicinal liquid is the ultrafilter membrane ultrafiltration of 10000 molecular weight through flux, packing, and every bottle of 5ml, lyophilizing, gland, label promptly gets the powder pin, totally 10000 bottles.
Claims (2)
1, a kind of effective ingredient in Chinese compound injection that is used for the treatment of cardio-cerebralvascular diseases, it is characterized in that by Borneolum Syntheticum and Folium Ginkgo extract by 1: the combination of 2-20 proportioning is made; The preparation technology of Folium Ginkgo extract is: the Folium Ginkgo of getting certain mass is with the finite concentration alcohol reflux, concentrating under reduced pressure, after the clarifier sedimentation, concentrating under reduced pressure is with the 50% left and right sides ethanol dried coarse powder of desilver Folium Pruni that boils again, adsorption resin column on the filtrate, get pale yellow powder shape extract at last, contain flavonoid glycoside and Ginkgo total lactones and be not less than 24%, 6% respectively.
2, a kind of preparation method of described effective ingredient in Chinese compound injection as claimed in claim 1 is characterized by:
One; get Borneolum Syntheticum 1 weight portion; fine ground; add 30-70 weight portion glycerol heated and stirred and make fully dissolving; be standby; as to get Folium Ginkgo extract 2-20 weight portion; add an amount of water for injection dissolving, the glycerite of above-mentioned Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, stir; the osmotic pressure regulator that milliosmolarity such as need add to when making transfusion makes dissolving; this osmotic pressure regulator is a mannitol; at least a in sodium chloride or the glucose, be diluted to certain volume with proper amount of water for injection, mixing; medicinal liquid is the ultrafilter membrane ultrafiltration of 10000～30000 molecular weight through flux; embedding becomes injection or transfusion, sterilization promptly gets liquid drugs injection; infusion preparation;
Two; get Borneolum Syntheticum 1 weight portion; fine ground; after adding an amount of anhydrous alcohol solution; slowly add with 8-15 weight portion HP-and be made in the saturated aqueous solution; be incubated 40-60 ℃; stir or ultrasonicly make the solution clarification; other gets Folium Ginkgo extract 2-20 weight portion; add an amount of water for injection dissolving, the HP-enclose solution of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract, stir; the osmotic pressure regulator that milliosmolarity such as need add to when making transfusion makes dissolving; this osmotic pressure regulator is a mannitol; at least a in sodium chloride or the glucose, be diluted to certain volume with proper amount of water for injection, mixing; the ultrafilter membrane ultrafiltration of medicinal liquid via hole diameter 30000～100000 molecular weight; embedding becomes injection or primary infusion, sterilization promptly gets injection; the primary infusion preparation;
Three; get Borneolum Syntheticum 1 weight portion; fine ground; after adding an amount of anhydrous alcohol solution; slowly add with 8-15 weight portion HP-and be made in the saturated aqueous solution; be incubated 40-60 ℃; stir or ultrasonicly make the solution clarification; other gets Folium Ginkgo extract 2-20 weight portion, adds an amount of water for injection dissolving, the HP-enclose solution of Borneolum Syntheticum is slowly added in the aqueous solution of Folium Ginkgo extract; stir; add an amount of forming agent again and make dissolving, this forming agent is a mannitol; at least a in HP-or the glucose, be diluted to certain volume with proper amount of water for injection; stir; the ultrafilter membrane ultrafiltration of medicinal liquid via hole diameter 30000～100000 molecular weight, ultrafiltrate packing, lyophilizing; gland promptly get the powder pin.
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| Title |
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| 中医药治疗冠状动脉粥样硬化性心脏病心绞痛概况 袁振敏，周瑛.《河北中医》，第25卷第9期 2003 * |
| 中药单方、提取物及单纯化合物治疗冠心病心绞痛的研究进展 李巨奇，劭丹珂.《现代中西医结合杂志》，第9卷第22期 2000 * |
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