CN100341480C - Personal care formulations containing keratin - Google Patents

Abstract

The application describes a range of personal care products that include a keratin protein fraction. The fraction may be intact or hydrolysed. It is preferably S-sulfonated. The content of the fraction may range from 0.001% to 50%. In most formulations its content will be less than 1% although in certain products such as nail care products, the content will be higher. A wide range of personal care products are described including shampoos, body gels and lotions, conditioners, creams and cosmetics generally.

Description

Comprise keratic personal care formulations

Invention field

The present invention relates to comprise keratic personal care formulations and their application in cosmetics.

Background of invention

Protein and derivant thereof are widely used in the personal care formulations, comprise the care formulations that is used on hair, skin and the fingernail.As the component of personal care formulations, protein plays many functions, comprises conditioning, film forming, as wetting agent and lubricant.The proteolysis that great majority are commonly used so that enough dissolubilities to be provided, is convenient to dissolve in the preparation.Especially true concerning keratin and since with protein in relevant crosslinked of the cysteine of height on the characteristic that exists, itself be exactly insoluble.Be known in the art many aminosal examples of (comprising keratin) that in personal care formulations, use.

WO9851265 discloses the protein of hydrolysis and derivant (especially sulfur content is high) thereof and has avoided application in the preparation of environment and chemical damage at the protection hair.The inventor of WO9851265 uses the protein of hydrolysis and the mixture of poly-amino cationics to prepare required preparation.

US4948876 has described a kind of S-sulfo group cysteine keratin peptide that produces by enzyme hydrolysis, as the adjuvant of woolen knitwear and hair-dyeing.The author uses enzymic digestion to prepare low-molecular-weight peptide, and obtains required dissolubility.

US4895722 has discussed the application of many keratin catabolites (comprising those that make by chemical hydrolysis and enzyme hydrolysis) in the preparation cosmetics.

Keratin fiber (as people's hair, woollen fabrics and other animal fiber) is made up of the complex mixture of protein involved, and described protein all is keratin sectors of breakdown.These protein can be divided into following several classes according to the 26S Proteasome Structure and Function in the fiber:

Described intermediate filament protein matter (IFP) is modal fibrous proteins in fibrocortex;

High-sulfur protein (HSP) is the globular protein of finding in the substrate of fibrocortex and epidermis;

High glycine-tyrosine protein matter (HGTP), the most common in fibrocortex.

The ultrastructure of keratin fiber is known in the art, and by R.C.Marshall, D.F.G.Orwin and J.M.Gillespie at " structure of mammal hard keratin and biochemistry " (Structure andBiochemistry of Mammalian Hard Keratin), Electron Microscopy Reviews, 4, describe in detail in 47,1991.In the prior art of described protein as the beauty treatment composition, used keratin constitutes component as a kind of material hydrolysis and need not that the keratin source is divided into it.Since proteolysis, required numerous protein performance loss.The low-molecular-weight keratin peptide is reunited with the lower degree of order, forms its physical property ratio by the worse material of its deutero-high molecular weight keratin.In addition, follow the chemical method that keratin decomposes and the irreversible transformation of the cysteine that takes place has produced peptide prod, it has lost the core degree of functionality, and this can make it to distinguish mutually with other protein material.

Personal care formulations requires to use complete keratin, and it has kept many proper characteristics of natural keratin, and has the reactivity to the keratin base material.

Goal of the invention

The purpose of this invention is to provide the keratic personal care formulations of a kind of use, or being at least the public provides a kind of useful selection.

Summary of the invention

The invention provides a kind of segmental personal care formulations of keratin that comprises.

Described keratin fragment can be complete.

The present invention also provides a kind of personal care formulations, and wherein, described keratin fragment is hydrolyzed.

The present invention especially provides the sulfonated keratin of a kind of S-of comprising segmental personal care formulations.

The invention provides a kind of personal care formulations, wherein, described keratin fragment is from intermediate filament protein matter family.

The present invention also provides a kind of personal care formulations, and wherein, described keratin fragment is from high-sulfur protein family.

Described keratic cysteine content is about 4%.

The present invention also provides a kind of personal care formulations, and wherein, described keratin fragment is from high glycine-tyrosine protein matter family.

In described preparation, the complete segmental percentage ratio of S-sulfonation keratin is better less than 10 weight %.

Described ratio is more preferably 0.001-1 weight %.But described ratio can be the keratin fragment of 0.001-50%.

The present invention also provides a kind of segmental personal care formulations of about 0.001-50% keratin that comprises.

Described ratio better is 0.001-10%, is more preferably 0.001-1%.

The present invention also is provided for comprising the additive of the segmental personal care formulations of keratin.

Described personal care formulations comprises following:

Hair conditioning shampoo, health/facial cleansing agent/shampoo, hair care agent, hair jelly, mousse, style keeping liquid, eruption setting agent, solution before the hair-waving, hair-waving back solution, moisturiser, bath gel, foaming bath gels, mascara, nial polish, liquid foundation, shaving cream and lip pomade.

But, the present invention includes other personal care formulations.

The present invention also provides a kind of complete segmental personal care formulations of sulfonation keratin that comprises, and wherein, the segmental ratio of keratin is about 10% of described preparation.Described preparation is suitable for use as nial polish or fingernail glazing agent.

Described personal care formulations comprises the cosmetics carrier of approrpiate wts %.

Can add other composition improve as vitamin and inorganic matter as described in the protection effect of preparation.

Also can add sun protection factor with ultraviolet light protective nature.

The present invention also provides a kind of method of using personal care formulations of the present invention or additive.

Accompanying drawing describes in detail

Now, only the present invention is described by embodiment, wherein:

Fig. 1 has shown the Instron result of the test of the hair-waving fiber of handling with 5%SIFP.

Fig. 2 has shown the Instron result of the test of the hair-waving fiber of handling with 2%SIFP.

Fig. 3 has shown the Instron result of the test of the hair fiber of the bleaching of handling with 5%SIFP.

Fig. 4 has shown the lax Instron result who sends out fiber who handles with 2%SIFP.

Fig. 5 has shown under 50% relative humidity SIFP, SHSP and the SPEP substance to the hair that do not damage and damage.

Fig. 6 has shown the moisture-keeping function (moisturisation) when the hair relative humidity increases, and described hair is the hair of handling with SIFP, SHSP and SPEP that does not damage and damage.

Fig. 7 has shown conventional surfactants and SIFP, SHSP and the SPEP foaming effect under the condition that has and do not exist EDTA, and described EDTA is made by the test of Wei Lin Shi blender.

Fig. 8 has shown the foaming effect of the shampoo formulation that has and do not have SIFP, SHSP and SPEP.

Fig. 9 is the subjective assessment general introduction of shampoo formulation under the condition that has and do not exist SIFP.

Detailed Description Of The Invention

Described hard alpha-Keratin can be according to its biochemical property such as the alpha-Keratin from human hair, woollen fabrics, animal origin, angle, hoof or other mammal, and especially its molecular weight and amino acid form and is divided into concrete component. Table 1 has illustrated that the amino acid of determining by the conventional method of analysis of general keratin fragment known in the art forms, and this also is theme of the present invention. This relates to the acid hydrolysis of described analyte, and described acid hydrolysis changes into cysteine with all cystines and unstable cysteine derivative, is often registered as half-cysteine.

	SIFP and SIFP-pep	SHSP and SHSP-pep	SPEP	IFP	HSP	HGTP	Complete woollen fabrics
								Cya	0.4	1.7	0.7	0	0	0	0
Asp	7.9	2.6	8	9.6	2.3	3.3	5.9
								Glu	15.4	8.6	15	16.9	7.9	0.6	11.1
Ser	10.9	14.3	11.4	8.1	13.2	11.8	10.8
								G1y	8.1	9.1	8.4	5.2	6.2	27.6	8.6
His	0.9	0.8	0.9	0.6	0.7	1.1	0.8
								Arg	7.9	6.8	6.9	7.9	6.2	5.4	6.2
Thr	6.5	10.4	6.5	4.8	10.2	3.3	6.5
								Ala	7.5	3.6	7.5	7.7	2.9	1.5	5.2
Pro	5.4	12.6	5.7	3.3	12.6	5.3	6.6
								Tyr	1.1	1.8	1.2	2.7	2.1	15.0	3.8
Val	6.5	6.3	5.8	6.4	5.3	2.1	5.7
								Met	0.2	0	0.3	0.6	0	0	0.5
Lan	0.2	0.2	0.3	0	0	0	0
								Ile	3.7	2.9	3.4	3.8	2.6	0.2	3
Leu	8.9	3.9	8	10.2	3.4	5.5	7.2
								Phe	2.5	1.5	2.1	2	1.6	10.3	2.5
Lys	2.1	0.4	2.1	4.1	0.6	0.4	2.7
								Cys	4.2	12.4	4.6	6	22.1	6.0	13.1

Table 1 has illustrated that the amino acid of keratin fragment forms: SIFP matter (SIFP), the peptide (SIFP-pep) that derives from SIFP matter, S-sulfonation keratin High-sulphur protein matter (SHSP), the peptide (SHSP-pep) that derives from S-sulfonation keratin High-sulphur protein matter, S-sulfonation keratin peptide (SPEP) that the present invention is used. Intermediate filament protein matter (IFP), High-sulphur protein matter (HSP), high Gly-Tyr protein (HGTP) and Gillespie and Marshall are at " mutability in the protein of woollen fabrics and hair " (Variability in the proteins of wool and hair), Proc.Sixth Int.Wool Text. Res.Conf., Pretoria, 2,67-77, the complete woollen fabrics described in 1,980 one literary compositions. All residues are explained with mol%. S-sulfo group cysteine, cystine and cysteine are measured with S-carboxyl methyl cysteine, reduce afterwards and alkylation, and are designated as cys.

Table 2 has illustrated the molecular weight of determining by the conventional method of analysis of general keratin fragment known in the art, and this also is theme of the present invention. Traditional analysis method relates to uses reduction reaction that the cystine linkage in the keratin is cut off, and determines to be the quality of the protein (being similar to the not protein of keratinization state most) of natural, non cross-linked state. Use the polyacrylamide gel electrophoresis quality measurement. In the situation of peptide SPEP, use mass spectrograph to determine quality. Use these methods, described keratin is the solubility preparation under the condition of not carrying out any peptide bond hydrolysis, and has measured the accurate measured value of molecular weight.

The keratin fragment	Molecular weight/kD
		SIFP	40-60
SHSP	10-30
		SPEP，SPEP-pep，SHSP-pep	＜1
IFP	40-60
		HSP	10-30
HGTP	＜10

Table 2: the molecular weight of keratin fragment: SIFP matter (SIFP), the peptide (SIFP-pep) that derives from SIFP matter, S-sulfonation keratin High-sulphur protein matter (SHSP), the peptide (SHSP-pep) that derives from S-sulfonation keratin High-sulphur protein matter, S-sulfonation keratin peptide (SPEP) that the present invention is used. Intermediate filament protein matter (IFP), High-sulphur protein matter (HSP), high Gly-Tyr protein (HGTP) and Gillespie and Marshall are at " mutability in the protein of woollen fabrics and hair " (Variability in the proteins of wool and hair), Proc.Sixth Int.Wool Text.Res.Conf., Pretoria, 2,67-77, the complete woollen fabrics described in 1,980 one literary compositions.

Amino acid forms and molecular weight changes with the keratin type, between the kind and also in the kind of a kind, for example between the woollen fabrics from different sheep varieties. Table 1 and 2 given numerical value have been indicated described keratin source. But keratin or the keratin fragment of type take on a different character separately, and especially molecular weight and amino acid form.

Theme of the present invention is the preparation that comprises complete S-sulfonation keratin fragment. " complete " refers to not significantly hydrolysis of protein, and described hydrolysis is defined as by adding entry and makes bond fission. Gillespie is at " biochemistry of skin and physiology " (Biochemistry and physiology of the skin), volume 1, Goldsmith writes Oxford University Press, London, 1983, the 475-510 page or leaf) think that " complete " refers to be the protein of angling polymerization state, also represent the polypeptide subunit, they are compounded to form the complete keratin in woolen knitwear and the hair. In the present invention, " complete " refers to the described polypeptide subunit of Gillespie. These are equivalent to the keratin of its native form, by the angling process form two sulfur-crosslinked.

The keratin fragment is visibly different class in the keratin family, as known in the art intermediate filament protein matter, High-sulphur protein matter or high Gly-Tyr protein. Intermediate filament protein matter such as Orwin etc. describe (Electron Microscopy Reviews in detail in " structure of mammal hard keratin and biochemistry " (Structure and Biochemistry of Mammalian Hard Keratin) literary composition, 4,47,1991), refer to that also Gilliespie is at " biochemistry of skin and physiology " (Biochemistry and physiology of the skin), volume 1, Goldsmith writes Oxford University Press, London, 1983,475-510 page or leaf) described low prot th. The key feature of this protein family is that molecular weight is 40-60D, and cysteine content is about 4%. Described High-sulphur protein matter family also such as Orwin and Gillispie described in the same publication. This protein family has the heterogeneity of height, but it is characterized in that molecular weight is 10-30kD, and cysteine content is greater than 10%. The content of the cysteine of this ethnic subclass (ultra-high-sulfur(UHS) protein) is up to 34%. Described high Gly-Tyr protein family also such as Orwin and Gillispie described in the same publication. This race is also referred to as high tyrosine protein matter, it is characterized in that molecular weight less than 10kD, and tyrosine content is usually greater than 10%, and glycine content is usually greater than 20%.

In the present invention, " keratin fragment " is keratic pure form, and it mainly comprises (although and not exclusively comprising) above-mentioned a kind of different protein group. In the present invention, S-sulfonation keratin has the cysteine/cystine that mainly is S-sulfo group cysteine form, is commonly referred to Bunte salt. This high polarity group is given protein with solubility, although stable in solution, described S-sulfo group is unsettled cysteine derivative, is easy to and thio-alcohol (such as cysteine) and the reaction of other reducing agent. Cause S-sulfo group cysteine again to be converted into cysteine with the reducing agent reaction. S-sulfo group cysteine is chemically different with cysteic acid, although both all comprise SO₃ ^-Base. Cysteic acid irreversibly produces by oxidation cysteine or cystine, in case form, just can not be with the two sulfur-crosslinked cysteines that again form. S-sulfo group cysteine reacts with cysteine easily, forms easily two sulfur-crosslinked.

One aspect of the present invention is the personal care formulations that comprises SIFP matter (SIFP). These protein are characterised in that molecular weight is 40-60kD, and cysteine content determines to be about 4% by amino acid analysis. These materials can prepare by the whole bag of tricks, comprise as described in the NZ/PCT02/00125. These materials have good film-forming quality, and (such as described those methods of NZ/PCT02/00169) form again in every way. At least part of complete character from celloglobulin of described material character. Known intermediate filament protein matter is relevant with molecular level, is the basis that protein is formed again material. This material is useful beauty treatment character as the ability of film forming agent. In addition, described S-sulfo group is used in the personal care formulations, at this moment because its easy and thio-alcohol reaction forms covalent disulfide bonds. Thio-alcohol is the form of cysteine, especially in the hair that damages by method of reducing such as hair-waving. In addition, as high polar group, described S-sulfo group invests the hair surface of polar substrates as damaging because of oxidizing process and bleaching. For such hair, SIFP can form salt bridge and hydrogen bond, thereby gives lasting hair care effect.

The present invention is the cosmetic formulation that comprises S-sulfonation keratin High-sulphur protein matter (SHSP) on the other hand. These protein are characterised in that molecular weight is 10-30kD, and the cysteine content of determining by amino acid analysis is greater than 10%. This material can prepare by the whole bag of tricks (comprising the described method of NZ/PCT02/00125), full spherical protein for the matrix protein matter that comes from the keratin fiber cortex and epidermal cell, this material has the ability of repairing damaged hair, and especially this complete protein can infiltrate in the described fiber at the furcated end place. In addition, compare commercially available keratin derivatives, the ratio of cysteine commonly used is higher in the personal care formulations, when as the permanent waveform method of part, is attached to damaged hair or the ability that is attached on the shape hair is significant.

One aspect of the present invention is the keratin peptide from the keratin fragment. These peptides have the cysteine content that is similar to the component that peptide derives (about 4% concerning SIFP-pep, concerning SHSP-pep greater than 10%). Because molecular weight is low, these materials infiltrate the surface of hair and skin, provide cosmetology function in these base materials. Because derived from concrete keratin fragment and the cysteine that exists as S-sulfo group cysteine, these materials are different with other hydrolysis of keratin. Peptide source with variable cysteine is valuable especially in the cosmetic formulation.

One aspect of the present invention is the personal care formulations that comprises from large keratic S-sulfonation keratin peptide. These peptides are characterised in that molecular weight is about 1kD or following, and the cysteine content of determining by amino acid analysis is about 4%. This material can prepare by the whole bag of tricks (comprising the described method of NZ/PCT02/00125). Because cysteine exists with S-sulfo group form, this material is different from other hydrolysis of keratin. The material that this molecular weight is low can be by the infiltration of hair epidermis. This feature (the S-sulfo group that exists on the binding peptide and the reactivity of this group) forms a kind of for the cosmetic formulation especially composition of hairdressing product.

The keratic cysteine content that is characterised in that is higher than other oroteins. In some protein fragments from wool, cysteine content is up to 30%. Cysteine is a kind of known reducing agent, keratin components of the present invention is can be as reducing agent and the antioxidant of active component in the personal care formulations, is used for anti-ageing or reduces the hair that caused by free radical, pollutant and environmental injury and the oxidative damage of skin. The non-oxidizability of keratin fragment is measured as described in Table 3.

Sample	The antioxidation activity of surveying	The equivalent of 100% protein is active
			SPEP	281.86μmol TEAC/100mL	1879μmol TEAC/100mL
SIFP	207.92μmol TEAC/100mL	4158μmol TEAC/100mL
			SHSP	850μmol TEAC/100mL	5667μmol TEAC/100mL
The SIFP powder	2196μmol TEAC/100g	2196μmol TEAC/100g

Table 3: the non-oxidizability of keratin fragment. The result is expressed as the antioxidant capacity (μ mol TEAC/100g or μ mol TEAC/100mL) of Trolox equivalent in per 100 grams or the ml sample, and expression and 100g or ml sample have the Trolox amount (vitamin E) of same response. Each extract is carried out three analytes (with different concentration). Content take the protein concentration of specimen in use as the basis active (SPEP and SHSP15% solution; SIFP 5% solution).

Personal care formulations comprises any material or the preparation that will contact with any exterior section (comprising oral mucosa and tooth) of human body, and purpose is:

The smell of-change health;

Its outward appearance of-change;

-cleaning;

Its kilter of-maintenance; Or

-Fa is fragrant;

But, do not comprise the product of thinking medicine, therapeutant or utensil, food or nutrition or dietary supplement legally.

Also be included as any personal care formulations that improves described outward appearance.

Unless this paper explicitly calls for, in whole description and claims, word " comprises " etc. and to be meant the meaning that is included, with eliminating or exhaustive opposite, be " including but not limited to " consciousness.

Only pass through embodiment now, and the embodiment of reference example illustrates the present invention.

Embodiment

In each preparation, comprise " the keratin fragment " that to represent content.The keratin fragment is meant the sulfonated keratin peptide of SIFP, SIFP-pep, SHSP, SHSP-pep, HGTP or S-, and all as mentioned above.Unless have describedly in addition, provide the keratin fragment with the dilute aqueous form, and in preparation, comprise this solution of appropriate amount, obtain indicated keratin content of fragment.The concentration that is used for the general aqueous solution of keratin clip types is SIFP 5%, SHSP 15% and the sulfonated keratin peptide 15% of S-.Therefore, for make SIFP reach 0.5% keratin segmental shown in content, in preparation, to use 10% SIFP.Percentage table is shown w/v.

Sample formulation

Hair conditioning shampoo

Sodium lauryl sulfate 28% 25.0%

Lauryl polyoxyethylene-2-ether sodium sulfate 70% 4.0%

Cocoyl diethanolamine 70% 3.5%

Cocoamidopropyl (30%) 3.0%

Keratin fragment 0.5%

Sodium chloride is an amount of

Citric acid is an amount of

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

Step: A mixes 35.0g water, sodium laureth sulfate (Sodium laureth sulphate) and sodium lauryl sulfate; Be heated to 65 ℃ up to dissolving.Add cocoyl diethanolamine (cocamideDEA), and make it cooling.B mixes betanin and water, and adds among the phase A.Add the keratin fragment, with citric acid with pH regulator to 6.5.Add required antiseptic and spice, be adjusted to required thickness, and add remaining water with sodium chloride.

Hair jelly

Carbomer (carbomer, Carbopol Ultrez 10) 0.5%

EDTA disodium 0.05%

Glycerol 4.0%

Triethanolamine (20%) 3.0

Keratin fragment 0.45

Antiseptic is an amount of

Spice is an amount of

Water is in right amount to 100%

Step: A is heated to 70 ℃ with 60.0g water, and adds Carbopol, EDTA and glycerol.The violent mixing, cooling.Add triethanolamine with pH regulator to 6.3.Add the keratin fragment.Mix antiseptic and remaining water, and add.The violent mixing, and add required spice.

Bright and clean health/facial cleansing agent and shampoo

Ammonium lauryl sulfate 28% 25.0%

Laureth sulfo-succinic acid disodium 20.0%

Cocos nucifera oil acylamino-(cocamido) CAB 8.0%

Keratin fragment 0.5%

Sodium chloride is an amount of

Citric acid is an amount of

Spice (parfum) is an amount of

Antiseptic is an amount of

Water (aqua) is in right amount to 100%

The hair care agent

Hexadecyltrimethylammonium chloride 5.0%

Stearyl alcohol 4.5%

Keratin fragment 0.25%

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

Send out mousse

Keratin fragment 0.25%

H-tallow base trimethyl ammonium chloride 0.20%

Nonyl pheno (10) ether 0.35%

Alcohol 10.0%

Butane-48 10.0%

Water is in right amount to 100%

Style keeping liquid

Carbomer (Carbopol Ultrez 10) 2.0%

Mineral oil (lightweight) 0.20%

Keratin fragment 0.25%

Alcohol 37.5%

Spice is an amount of

Water is in right amount to 100%

The eruption setting agent

VA/ .beta.-methylacrylic acid esters/vinyl neodecanoic acid copolymer 1 .60%

(Resyn 28-2930)

Amino methyl propanol 0.15%

PEG-75 lanoline 0.20%

Keratin fragment 0.25%

Alcohol 65.05%

Butane 30 28.0%

Solution before the hair-waving

Triethanolamine lauryl sulfate 30.0%

Cocos nucifera oil acylamino-propyl-dimethyl amine oxide 10.0%

Cocoyl diethanolamine 7.5%

Cocoamidopropyl 20.0%

Cocoyl monoethanolamine 3.0%

Keratin fragment 0.5%

Spice is an amount of

Antiseptic is an amount of

Water is an amount of

Hair-waving back solution

Keratin fragment 0.5%

Cocos nucifera oil acylamino-propyl-dimethyl amine oxide 10.0%

Cetyl polyoxypropylene (5) polyoxyethylene (10) polyether phosphate 0.5%

(PPG-5-ceteth-10-phosphate)

Glycerol 3.0%

HYDROXY PROPYL METHYLCELLULOSE 1.5%

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

Moisturiser

The pure and mild 16/octodecyl alcohol polyoxyethylenes of 16/18 (cetaaryl) (20) ether

(ceteareth-20) 5.0％

16/octadecanol 2.0%

Mineral oil (lightweight) 5.0%

Keratin fragment 0.5%

Antiseptic 0.3%

Spice is an amount of

Water is in right amount to 100%

Handwashing liquid and refreshing body water

Polyglyceryl-3 methyl glucoside distearate 4.0%

Octadecyl/Shan Yu base Cera Flava (beeswaxate) 3.0%

Octyl dodecanol 4.0%

American Avocado Tree oil 6.0%

Mineral oil 3.0%

Simmondsia chinensis oil 2.0%

Keratin fragment 0.5%

Ceramide III 0.2%

Propylene glycol 3.0%

Antiseptic is an amount of

Spice (parfum) is an amount of

Water (aqua) is in right amount to 100%

Wrinkle resistant facial cream

Shan Yu acyl group sodium lactate 2.0%

16/octadecanol 3.0%

Tristerin 2.6%

Isopropyl palmitate 6.0%

Oleum Helianthi 6.0%

Keratin fragment 0.5%

Glycerol 3.0%

Magnesium Ascorbyl Phosphate (with) lecithin 6.0%

(Rovisome-C，R.I.T.A)

Antiseptic is an amount of

Water is in right amount to 100%

Facial moisturiser

Tetradecyl lactate 3.0%

Lanolin alcohol polyoxyethylene (25) (Laneth-25) and cetyl polyoxyethylene (25) ether 1.0%

(Ceteth-25) and oil base polyoxyethylene (25) ether (oleth-25) and stearyl polyoxyethylene (25) ether

(Steareth-25)(Solulan 25，Amerchol)

Mineral oil (70 viscosity) 16.5%

Vaseline 3.0%

Tocotrienol (Tocotrienol) 1.0

Carbomer 934 0.75%

Keratin fragment 0.5%

Triethanolamine (10% aqueous solution) 7.5%

Antiseptic is an amount of

Spice is an amount of

Water is in right amount to 100%

The refreshing body water of preserving moisture

Methyl glucoside dioleate 2.0%

Glucate SS 1.5%

Methyl gluceth (gluceth)-20 distearate 1.5%

16/octadecanol (with) 16/octodecyl alcohol polyoxyethylene (20) ether (Ceteareth-20) 1.5

Isopropyl palmitate 3.0%

Ceramide 3, hexyl Decanol 2.0%

Methyl gluceth (gluceth)-10 3.0%

Keratin fragment 0.5%

Carbomer 1,342 0.2%

Triethanolamine 0.2%

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

The cation emollient

Isostearoyl aminopropyl lauryl acetyl alkyl dimethyl ammonium chloride 5.0%

Lactamide MEA 3.0%

Neopentanoic acid iso stearyl ester 15.0%

Myristic acid Fructus Amomi Rotundus ester 1.0%

Spermol 4.0%

Glyceryl isostearate 3.5%

Keratin fragment 0.5%

Antiseptic is an amount of

Water is in right amount to 100%

The facial conditioner of man

Carbomer (Ultrez 10 Carbopol) 0.4%

Propylene glycol 1.0%

PPG-5-polyoxyethylene 0.55%

Beta glucan 2.0%

PEG-60 castor oil hydrogenated 0.5%

Triethanolamine (99%) 0.4%

Keratin fragment 0.5%

SD-39C alcohol (Quantum) 5.0%

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

The back wetting agent shaves

16/octodecyl alcohol polyoxyethylene-12 (with) 16/octodecyl alcohol polyoxyethylene-20 6.0%

(with) 16/octadecanol (with) cetin (with)

Tristerin (Emulgade SE, Henkel)

16/octadecanol 1.0%

Two caprylyl ethers 8.0%

Octyldodecanol 4.0%

Glycerol 3.0%

Carbomer (Ultrez 10 Carbopol) 0.3%

Keratin fragment 0.5%

Bisabolol 0.2%

Ethanol 3.0%

Water (with) hyaluronate sodium (with) Semen Tritici aestivi 4.0%

(common wheat) embryo tooth extract (with) saccharomyces cerevisiae

Extract (Eashave, Pentapharm)

Triethanolamine is an amount of

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

The antioxygen defrost

Glycerol (99.7%) 3.0%

Xanthan gum 0.15%

EDTA disodium 0.05%

Parleam 1.0%

Isopropyl palmitate 5.0%

Vaseline 0.75%

Dimethicone 0.75%

Encircle penta siloxanes 3.0%

Stearyl polyoxyethylene (2) ether (Steareth-2) 1.0%

PEG-100 stearate 1.9%

Spermol 2.0%

Ethylhexyl palmitate 3.0%

Polyacrylamide (with) C13-14] isoparaffin (with) 2.0%

(sepigel 305, Seppic) for lauryl polyoxyethylene (7) ether (laureth-7)

Keratin fragment 0.5%

Glycerol (with) water (with) European grape (vitis vinitera) 0.5%

Plant seed extract (Collaborative)

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

Liquid detergent

Lauryl polyoxyethylene sodium sulfate 50.0%

Cocoyl diethanolamine 3.0%

Keratin fragment 0.25%

Sodium chloride is an amount of

Antiseptic is an amount of

Citric acid is an amount of

Water is in right amount to 100%

Bath gel

Lauryl polyoxyethylene sodium sulfate 35.0%

Sodium N-lauroyl sarcosinate 5.0%

Cocoamidopropyl 10.0%

Cocos nucifera oil acylamino-propyl hydroxy sulfobetaines 5.0%

Glycerol 2.0%

Keratin fragment 0.15%

EDTA four sodium 0.25%

Citric acid is an amount of

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

The foaming bath gels

Triethanolamine lauryl sulfate 40.0%

Lauroyl diglycollic amide 10.0%

Linoleic acid diglycollic amide 7.0%

PEG-75 lanolin oil 5.0%

Keratin fragment 0.25%

EDTA four sodium 0.5%

Spice is an amount of

Antiseptic is an amount of

Dyestuff is an amount of

Water is in right amount to 100%

Nial polish

For this embodiment, being convenient to provides the keratin fragment with the S-sulphonic form of dried powder.

First coating

Keratin fragment (SIFP) 10.0%

Sodium hydroxide (4%) 10.0%

Keratin fragment (SHSP or SPEP) is an amount of

Sodium lauryl sulfate is an amount of

Dyestuff or pigment are an amount of

Water is in right amount to 100%

The fingernail glazing agent

Keratin fragment (SIFP) 10.0%

Keratin fragment (SHSP or sulfonated keratin peptide) is an amount of

Sodium hydroxide (4%) 10.0%

Sodium lauryl sulfate is an amount of

Water is in right amount to 100%

Sclerosing agent

Citric acid 21.0%

Water 79.0%

Mascara

PEG-8 3.0％

Xanthan gum 0.50%

Tetrahydroxypropyl ethylenediamine 1.3%

Brazil wax 8.0%

Cera Flava 4.0%

Isoeicosane 4.0%

Polyisobutylene 4.0%

Stearic acid 5.0%

Tristerin 1.0%

Keratin fragment 0.25%

Pigment 10.0%

Polyurethane-1 8.0%

VP/VA copolymer 2.0%

Antiseptic is an amount of

Spice is an amount of

Water is in right amount to 100%

Liquid foundation

Polysorbate80 0.1%

Potassium hydroxide 0.98%

Keratin fragment 0.25%

Titanium dioxide/Talcum, 80% 0.1%

Talcum 3.76%

Yellow iron oxide/Talcum, 80% 0.8%

Red iron oxide/Talcum, 80% 0.38%

Black iron oxide/Talcum, 80% 0.06%

Propylene glycol 6.0%

Magnesiumaluminumsilicate 1.0%

Cellulose gum 0.12%

Two-PPG-3 Fructus Amomi Rotundus ether adipate ester 12.0%

16/octadecanol (with) cetyl polyoxyethylene (20) ether (ceteth-20) 3.0%

Phosphoric acid (with) di(2-ethylhexyl)phosphate cetyl ester (Crodafos CS 20 Acid)

Stearyl polyoxyethylene (10) ether (Steareth-10) 2.0%

Spermol 0.62%

Stearyl polyoxyethylene (2) ether (Steareth-2) 0.5%

Antiseptic is an amount of

Water is in right amount to 100%

Shaving cream

Cocos nucifera oil sodium sulfate 5.0%

Keratin fragment 0.25%

Glycerol 7.0%

Lauryl sulfo-succinic acid disodium 50.0%

The EDTA disodium is an amount of

Sodium chloride is an amount of

Citric acid is an amount of

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

Lip pomade

Octyl dodecanol 22.0%

Oleyl alcohol 8.0%

Keratin fragment 0.16%

C30-45 alkyl methyl siloxanes (methicone) 20.0%

Lanolin oil 14.0%

Vaseline 5.0%

Bentonite 36 (Rheox) 0.6%

Tenox 20(Eastman) 0.1％

Pigment/Oleum Ricini 10.0%

Antiseptic is an amount of

Ring-type methylsiloxane (cyclomethicone) is in right amount to 100%

The agent of sulphite hair straightening

Carbomer (Carbopol 940) 1.5%

Ammonium hydrogen sulfate 9.0%

Diethylidene urea 10.0%

16/octodecyl alcohol polyoxyethylene (20) ether (Cetearth 20) 2.0%

Keratin fragment 0.5%

Spice is an amount of

Ammonium hydroxide 28% is an amount of to pH7.2

Water is in right amount to 100%

Curly hair straightens post neutralization solution

Sodium bicarbonate 2.35%

Sodium carbonate 2.94%

EDTA 0.15％

16/octodecyl alcohol polyoxyethylene (20) ether 0.2%

Keratin fragment 0.5%

Spice is an amount of

Water is in right amount to 100%

Pre-curly hair aligning hair conditioner

Cationic polyamine 2.0%

Imidazolidinyl urea 0.25%

Keratin fragment 0.5%

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

The agent (Lye) of alkali metal hydroxide aligning

Bentonite 1.0%

Sodium lauryl sulfate 1.5%

PEG-75 lanoline 1.5%

Vaseline 12.0%

16/octadecanol 12.0%

Sodium hydroxide 3.1%

Keratin fragment 0.5%

Spice is an amount of

Water is in right amount to 100%

The back shampoo of curly hair aligning

Sodium lauryl sulfate 10.0%

Cocos nucifera oil ether diethanolamine 3.0%

EDTA 0.2％

Keratin fragment 0.5%

Citric acid arrives pH5.0 in right amount

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

Hair oil/epidermis overlay film

Glycerol 5.5%

EDTA 0.07％

Carbomer (Carbopol Ultrez 10) 0.33%

Triethanolamine (20%) 1.0%

Keratin fragment 0.5%

Ethanol 10.0%

Antiseptic is an amount of

Water is in right amount to 100%

Stay the hair conditioner on the hair

Spermol 5.0%

Tristerin 3.0%

Vaseline 0.7%

Isopropyl myristate 1.5%

Polysorbate60 1.0%

Dimethicone (dimethiconol) and ring-type methylsiloxane (cyclomethicone) 4.0%

Glycerol 7.0%

EDTA 0.1％

D-panthenol 0.2%

Keratin fragment 0.5%

Ring-type methylsiloxane 10.0%

Spice is an amount of

Antiseptic is an amount of

Water is in right amount to 100%

Hair conditioner after marcelling

Poly-(dimethyl diallyl ammonium chloride) (Quaternum-40) 2.0%

Keratin fragment 0.5%

Amphoteric-2 4.0％

Hydroxyethyl-cellulose 2.0%

Phosphoric acid arrives pH4.5 in right amount

Spice is an amount of

Water is in right amount to 100%

Temporary hair dyeing shaping glue

Dimethicone copolyol (dimethicone copolyol) 1.5%

PPG-10 methyl glucose ether 1.0%

Polyvinyl pyrrolidone 2.5%

Triisopropanolamine 1.1%

Carbomer (Carbopol 940) 0.6%

Lauryl polyoxyethylene (23) ether (Laureth-23) 1.0%

Phenyl phenol 0.2%

Keratin fragment 0.5%

EDTA 0.01％

D﹠amp; The orange dyestuff 4 0.12% of C

Ext D﹠amp; C cudbear 2 0.02%

FD﹠amp; C weld 6 0.02%

Ethanol 5.0%

Spice is an amount of

Water is in right amount to 100%

Comprise the beauty treatment performance that the segmental preparation of keratin can improve hair.This illustrates by following examples.

Embodiment 1: reinforce

The Instron method

Before measuring, hair fiber is placed water with the Instron cupping machine.Load unit 10N, loading range 10%, speed 30mm/min, gauge length 15mm.

50 fibers of record single hair fiber is prolonged 2% and 20% required energy, and average.

Material

Hair-waving solution

8% TGA, with ammonia spirit with pH regulator to 8

The hair-waving nertralizer

2.5% hydrogen peroxide

Liquid lime chloride

9% hydrogen peroxide, 1% Ammonium persulfate., pH8.3

Hair straightening (relaxing) solution

2.5% sodium hydroxide

Lax nertralizer

9.5% citric acid

The hair-waving step

1. be immersed in the hair-waving solution 3 hours from identical source (Caucasian's) hair fiber (～4cm, length).

2. placed neutralization solution 30 minutes, and air-dry.

3. place and comprise the segmental solution of an amount of keratin 30 minutes.

4. the fiber of cleaning treatment, drying, and under the situation of " flush away " step, in 50% relative humidity and 23 ℃ of following equilibrate overnight.Described cleaning step can save in the step of " staying on the hair ".

5. on the Instron device, measure and elongate required energy.

Blanching step

1. immersed in the liquid lime chloride 3 hours from identical source (Caucasian's) hair fiber (～4cm, length).

2. place and comprise the segmental solution of appropriate amount keratin 30 minutes.

3. clean, drying, and in 50% relative humidity and 23 ℃ of following equilibrate overnight.

4. on the Instron device, measure and elongate required energy.

Relaxation step

1. immersed in the lax solution 30 minutes from identical source (Caucasian's) hair fiber (～4cm, length).

2. placed neutralization solution 5 minutes, and in RO water, cleaned also air-dry.

3. place and comprise the segmental solution of an amount of keratin 30 minutes.

4. clean, drying, and in 50% relative humidity and 23 ℃ of following equilibrate overnight.

5. on the Instron device, measure and elongate required energy.

Test example 1: the hair-waving step that the keratin fragment of use 5%SIFP is carried out (supplying with), i.e. 0.25% activity as 5% aqueous solution.As above-mentioned Instron cupping machine.Result such as table 4 and shown in Figure 1.

Explanation	At 2% o'clock average energy (mJ)	Si Tudunte t checks (P)	At 20% o'clock average energy (mJ)	P
					Not damage	0.0406		3.718
Scalded	0.0382		3.543
					Clean	0.0491	＜0.001	4.030	＜0.02
Stay on the hair	0.0515	＜0.001	3.871	＜0.03

The boiling hot mistake that table 4 is handled with 5%SIFP and the Instron result of the test of unmarred hair fiber.The result is expressed as the 2% and 20% required average energy (milli is burnt) of hair fiber being elongated gauge length (15mm).

This studies show that by hair-waving and dies down and all use the hair fiber that comprises the segmental solution-treated of keratin to recover intensity in flush away and " staying on the hair " step.Use Si Tudunte t check to measure statistically, with respect to the fiber that scalded, with scalded/fiber that keratin is handled elongates required energy to be increased, all remarkable under the concurrent present all situations.

Test example 2: the hair-waving step that the keratin fragment of use 2%SIFP is carried out (supplying with), i.e. 0.1% activity as 5% aqueous solution.As above-mentioned Instron cupping machine.Result such as table 5 and shown in Figure 2.

Explanation	Average energy at 2% o'clock	P	Average energy at 20% o'clock	P
					Not damage	0.0316		3.252
Scalded	0.0278		3.100
					Stay on the hair	0.0357	＜0.001	3.325	＜0.054

Table 5 is with the boiling hot mistake of 2%SIFP processing and the Instron result of the test of unmarred hair fiber.The result is expressed as the 2% and 20% required average energy (milli is burnt) of hair fiber being elongated gauge length (15mm).

This studies show that, is handling (as the part of " staying on the hair " step) afterwards with segmental 0.1% living solution of keratin, and the hair fiber that scalded is tough.Use Si Tudunte t check to analyze described difference statistically, remarkable on the concurrent present statistics (in 2% overtime p＜0.001; In 20% overtime p＜0.054).

Test example 3: the blanching step that the keratin fragment of use 5%SIFP is carried out (supplying with), i.e. 0.25% activity as 5% aqueous solution.As above-mentioned Instron cupping machine.Result such as table 6 and shown in Figure 3.

Explanation	Average energy at 20% o'clock	P
			Not damage	3.610
Bleaching	3.610
			Stay on the hair	4.004	＜0.03

Table 6 is with the bleaching of 5%SIFP processing and the Instron result of the test of unmarred hair fiber.The result is expressed as the 20% required average energy (milli is burnt) of hair fiber being elongated gauge length (15mm).

This hair fiber that studies show that described bleaching comprises the segmental solution-treated of 0.25% active keratin (as a part of staying step on the hair) strength-enhanced afterwards in use.Use Si Tudunte t check to analyze described difference statistically, on the concurrent present statistics remarkable (p＜0.03).

Test example 4: the relaxation step that the keratin fragment of use 2%SIFP is carried out (supplying with), i.e. 0.1% activity as 5% aqueous solution.As above-mentioned Instron cupping machine.Result such as table 7 and shown in Figure 4.

Explanation	Average energy at 20% o'clock	P
			Not damage	3.610
Lax	2.997
			Flush away	3.378	＜0.015

Table 7, the Instron result of the test of the lax and unmarred hair fiber of handling with 2%SIFP.The result is expressed as the 20% required average energy (milli is burnt) of hair fiber being elongated gauge length (15mm).

This studies show that described hair fiber through the hair straightening step comprises the segmental solution-treated of 0.1% active keratin (as the part of flush away step) strength-enhanced afterwards in use.Use Si Tudunte t check to analyze described difference statistically, on the concurrent present statistics remarkable (p＜0.015).

Test routine 1-4 and confirmed that described keratin fragment is for providing enhanced effect (increasing as elongating the required energy of independent hair fiber) according to the hair that scalds, bleaches and align commonly used in the cosmetic treatments.

Embodiment 2: the fastness (substantivity) of hair conditioner on hair

Keratin shampoo formulation weight %

Ammonium lauryl sulfate (28%) 25.0

Laureth sulfosuccinic disodium 20.0

Cocoamidopropyl 8.0

Antiseptic 0.3

Keratin fragment 0.5

Sodium chloride is an amount of

Water is in right amount to 100%

Test procedure

Use the 2-3g hair sample.Carrying out described experiment repeats twice.

Clean described sample with shampoo before use, remove residual conditioner.

Described sample or not damage are perhaps repeatedly permed or blanching step.

The described sample of balance under 50%RH, and accurately weigh.

Or the keratin fragment is applied to (with the 3.0ml/ sample concentration) on the sample by aqueous solution or as the part shampoo formulation

With finger tip Treatment Solution is diffused on the sample, makes it to absorb 1 minute, in the RO current, clean.

Described sample is air-dry, and before weighing under 50%RH balance 24 hours.

The result is summarized in table 8 and Fig. 5.

	Average weight gain under 50% relative humidity (%)
				The keratin fragment	Explanation	Shampoo	Solution
SIFP	Bleaching	0.51	0.56
					Scalded	0.41	0.55
	Not damage	0.74	0.82
				SHSP	Bleaching	0.96	0.46
	Scalded	0.66	0.35
					Not damage	0.28	0.06
SPEP	Bleaching	0.72	2.10
					Scalded	0.50	1.70
	Not damage	0.0	0.0

Table 8 is handled and untreated damage or the weightening finish percent of unmarred hair under 50% relative humidity with solution that comprises SIFP, SHSP and SPEP or shampoo formulation.

Describedly studies show that described SIFP keratin fragment has substantial effect to the hair that does not damage, scalded and bleach from aqueous solution and shampoo formulation.Described SHSP keratin fragment is also to having substantial effect from aqueous solution and shampoo formulation, when applying as solution rather than shampoo, as if bleach and degree that the hair that scalded absorbs bigger.The segmental molecular weight of described keratin is less than 1kD, and to from the bleaching of aqueous solution and shampoo and the hair that scalded substantial effect being arranged, it doesn't matter but it increases with the weight of not damaging hair.Can observe weight by aqueous solution increases greatlyyer, shows that the surfactant that exists in the shampoo can remove the keratin fragment.

These results show that different keratin fragments have different surface activitys to hair fiber.Compare the little SPEP of molecular weight, bigger fragment has the big ability that forms absorbed layer, and transmits the effect of conditioning and smooth (gloss).

Embodiment 3 insulation effects

Experimental procedure

Use the 2-3g hair sample.In the experiment of carrying out, the each processing repeated twice.

Clean described sample with having high level of surfactant (not nursing one's health) shampoo before use, remove residual conditioner.

Described sample or not damage are perhaps repeatedly permed or blanching step.

The described sample of balance is 24 hours under 50%RH, and accurately weighs.

The described sample of balance is 24 hours under 73%RH, and accurately weighs.

Calculate because humidity increases the weight differential that (when not having protein to handle) causes.

Handle sample repetition twice (as mentioned above) with comprising the segmental aqueous solution of keratin or comprising the segmental shampoo of keratin.

The described sample of balance is 24 hours under 50%RH, and weighs.

The described sample of balance is 24 hours under 73%RH, and weighs.

Calculate because humidity increases the weight differential that (handling with keratin solution or shampoo) causes.

The result is summarized in table 9 and Fig. 6.

	The weight % that increases owing to moisture absorption during to 70%RH at 50%RH
						The keratin fragment	Explanation	The shampoo of prealbumen matter	The protein shampoo	The shampoo of prealbumen matter	The protein shampoo
SIFP	Bleaching	3.6	2.7	3.2	2.7
							Scalded	3.6	3.15	3.6	3.25
	Not damage	4.15	3.1	4.1	3.15
						SHSP	Bleaching	3.85	3.45	3.5	3.4
	Scalded	3.9	3.35	3.3	3.45
							Not damage	3.65	3.0	3.5	3.4
SPEP	Bleaching	3.85	4.4	4.1	4.1
							Scalded	3.95	4.55	4.05	4.1
	Not damage	2.7	4.3	2.75	3.8

Table 9 is with the damage of solution that comprises SIFP, SHSP and SPEP or shampoo formulation processing and the percetage by weight that unmarred hair increases with relative humidity.

The described moisture-keeping function that studies show that increases or reduces according to the keratin fragment that applies.When applying as aqueous solution or shampoo, described SIFP keratin fragment has reduced that scalded, bleaching and moisture absorption unmarred hair under high humility.

Described SHSP fragment is less to the influence of moisture absorption under high humility, when when applying, having some to show that moisture-keeping function reduces by shampoo (preferred aqueous solutions).

When applying shampoo, SPEP has especially increased moisture absorption.

Embodiment 4: the foaming of preparation

Test procedure

The test of Wei Lin Shi blender

Method:

1. the solution for preparing 100mL 5% test material.

2. pour in the blender.

3. the high speed fusion is 1 minute.

4. all liq being poured into 500mL measures in the cylinder.

5. the amount of foaming is (100mL) and record immediately for record.

6. write down the foam volume after 5 minutes, represent (this will draw " low bubble " and measure) with mL.

Test example 7. relatively has the segmental foaminess of keratin of conventional surfactants, and the effect that adds 0.5% metal ion chelation agent ethylenediaminetetraacetic acid (EDTA)

Use the test of Wei Lin Shi blender.

The result is summarized in table 10 and Fig. 7.

Explanation	Initial volume (mL)	Volume after 5 minutes
			Sodium lauryl sulfate (SLS)	635	595
Tween 20	275	215
			Triton X-100	365	345
CTAB	240	230
			SIFP	70	65
SIFP+EDTA	130	125
			SHSP	285	285
SHSP+EDTA	365	265
			SPEP	150	0
SPEP+EDTA	250	10

Table 10, foam volume and stability in the test of Wei Lin Shi blender.The result is expressed as after the fusion and immediately foam volume after 5 minutes.

This studies show that described SIFP keratin fragment shows gentle foaminess, and forms stable foam.Described SHSP fragment shows medium foaming capacity, and forms very stable foam.SPEP forms unsettled foam.Add metal ion chelation agent EDTA and improved all segmental foaming capacities.

Test example 8, the foam performance of keratin fragment mixture

The mixing angle protein fragments, and use Wei Lin Shi blender to estimate foaminess.

The result is summarized in table 11.

Explanation	Initial volume (mL)	Volume after 5 minutes
			4％SIFP，1％SHSP	220	210
2.5％SIFP，2.5％SHSP	175	165
			1％SIFP，4％SHSP	120	110

Table 11, the foam volume and the stability of keratin fragment mixture in the test of Wei Lin Shi blender.The result is expressed as after the fusion and immediately foam volume after 5 minutes.

This studies show that the SIFP keratin fragment that SHSP keratin fragment is added less foaming has improved foaming capacity.

Test example 9 comprises the foaming of the segmental shampoo formulation of keratin

Above-mentioned shampoo formulation comprises 0.5% active keratin fragment.

The result of Wei Lin Shi blender test is summarized in table 12 and Fig. 8.

Explanation	Initial volume (mL)	Volume after 5 minutes
			Shampoo is only arranged	450	435
The SIFP shampoo	450	440
			The SHSP shampoo	470	450
The SPEP shampoo	440	430

Table 12, foam volume and the stability of the shampoo that has and do not have SIFP, SHSP and a SPEP in the test of Wei Lin Shi blender.The result is expressed as after the fusion and immediately foam volume after 5 minutes.

Known described protein has adverse effect to the foaming in the preparation.This studies show that SIFP keratin fragment is added shampoo formulation can not have adverse effect to foaming, and some evidence shows that foam stability increases.And, the SHSP fragment is added shampoo formulation improved foaming capacity, not compare with there being keratic situation, the foam after 5 minutes is more.Described SPEP keratin fragment can not suppress foam and form.

Embodiment 5: the segmental subjective assessment of keratin in the shampoo formulation

Method

Provide two kinds of not shampoo formulations of label (as mentioned above) to the volunteer, the individual 0.5%SIFP keratin fragment that comprises of one.

Require the volunteer within a week, to have one's hair wash many times as usual, repeat with another sample then with a kind of sample.

Then, require the volunteer to do questionnaire survey, but each sample is graded according to foaming capacity, gloss generation, hair quality cardability and outward appearance.

Numerical value is less bad relevant with effect, for example, but under the situation of combing, the extremely difficult combing of 1=, but the 6=cardability is fabulous.

Test example 10

Collect questionnaire survey, the record score also is averaged.

The result is summarized in table 13 and Fig. 9.

Attribute	Only use shampoo (average)	Shampoo+SIFP keratin fragment (average)
			Foaming	4.8	5.0
Gloss	2.6	3.6
			Texture	2.6	4.2
But cardability	2.6	3.8
			Outward appearance	2.0	3.2

Table 13 has and does not have the subjective assessment of the shampoo formulation of SIFP.The result is the average that the volunteer writes down.

This studies show that the volunteer does not observe because of adding the main variation that the keratin fragment causes shampoo formulation to foam.And, provide superior gloss, texture cardability and improved outward appearance but observe the segmental preparation that exists for of keratin, show that it plays the effect of hair care.

Though with reference to above embodiment the present invention has been described, should have recognized under the condition that does not deviate from scope of the present invention and can carry out many improvement and modification.

Industrial applicability

Composition described in the application can be widely used in the personal care product, such as shampoo, gel, conditioner, creme and washing agent, comprises cosmetics, such as NMF, lotion, creme and gel.

Claims (42)

1. personal care formulations, it comprises 0.15% to 50% the sulfonated keratin fragment of S-.

2. personal care formulations as claimed in claim 1 is characterized in that, described keratin fragment is complete.

3. personal care formulations as claimed in claim 1 is characterized in that, described keratin fragment is hydrolysis.

4. as each described personal care formulations of claim 1-3, it is characterized in that described keratin fragment is an intermediate filament protein matter family.

5. as each described personal care formulations of claim 1-3, it is characterized in that described keratin fragment is from high-sulfur protein family.

6. personal care formulations as claimed in claim 4 is characterized in that, the segmental cysteine content of described keratin is 4%.

7. personal care formulations as claimed in claim 5 is characterized in that, the segmental cysteine content of described keratin is greater than 10%.

8. as each described personal care formulations of claim 1-3, it is characterized in that described keratin fragment is from high glycine-tyrosine protein matter family.

9. personal care formulations as claimed in claim 1 is characterized in that, it comprises the sulfonated keratin fragment of S-of 0.15%-10%.

10. personal care formulations as claimed in claim 1 is characterized in that, it comprises the sulfonated keratin fragment of S-of 0.15%-1%.

11., it is characterized in that described keratin fragment is complete as claim 9 or 10 described personal care formulations.

12., it is characterized in that described keratin fragment is hydrolysis as claim 9 or 10 described personal care formulations.

13. personal care formulations as claimed in claim 11 is characterized in that, described keratin fragment is from intermediate filament protein matter family.

14. personal care formulations as claimed in claim 12 is characterized in that, described keratin fragment is from intermediate filament protein matter family.

15. personal care formulations as claimed in claim 11 is characterized in that, described keratin fragment is from high-sulfur protein family.

16. personal care formulations as claimed in claim 12 is characterized in that, described keratin fragment is from high-sulfur protein family.

17., it is characterized in that the segmental cysteine content of described keratin is 4% as claim 13 or 14 described personal care formulations.

18., it is characterized in that the segmental cysteine content of described keratin is greater than 10% as claim 15 or 16 described personal care formulations.

19. personal care formulations as claimed in claim 11 is characterized in that, described keratin fragment is from high glycine-tyrosine protein matter family.

20. personal care formulations as claimed in claim 12 is characterized in that, described keratin fragment is from high glycine-tyrosine protein matter family.

21. an additive that is used for personal care formulations, it comprises the sulfonated keratin fragment of 0.15%-50%S-.

22. additive as claimed in claim 21 is characterized in that, described keratin fragment is complete.

23. additive as claimed in claim 21 is characterized in that, described keratin fragment is hydrolysis.

24., it is characterized in that described keratin fragment is from intermediate filament protein matter family as each described additive of claim 21-23.

25., it is characterized in that described keratin fragment is from high-sulfur protein family as each described additive of claim 21-23.

26. additive as claimed in claim 24 is characterized in that, the segmental cysteine content of described keratin is 4%.

27. additive as claimed in claim 25 is characterized in that, the segmental cysteine content of described keratin is greater than 10%.

28., it is characterized in that described keratin fragment is from high glycine-tyrosine protein matter family as claim 22 or 23 described additives.

29. additive as claimed in claim 21 is characterized in that, it comprises the S-sulfonation keratin fragment of 0.15%-10%.

30. additive as claimed in claim 21 is characterized in that, it comprises the S-sulfonation keratin fragment of 0.15%-1%.

31., it is characterized in that described keratin fragment is complete as claim 29 or 30 described additives.

32., it is characterized in that described keratin fragment is hydrolysis as claim 29 or 30 described additives.

33. additive as claimed in claim 31 is characterized in that, described keratin fragment is an intermediate filament protein matter family.

34. additive as claimed in claim 32 is characterized in that, described keratin fragment is an intermediate filament protein matter family.

35. additive as claimed in claim 31 is characterized in that, described keratin fragment is from high-sulfur protein family.

36. additive as claimed in claim 32 is characterized in that, described keratin fragment is from high-sulfur protein family.

37., it is characterized in that the segmental cysteine content of described keratin is 4% as claim 33 or 34 described additives.

38., it is characterized in that the segmental cysteine content of described keratin is greater than 10% as claim 35 or 36 described additives.

39. additive as claimed in claim 31 is characterized in that, described keratin fragment is from high glycine-tyrosine protein matter family.

40. additive as claimed in claim 32 is characterized in that, described keratin fragment is from high glycine-tyrosine protein matter family.

41. use the method for claim each described personal care formulations of 1-20 or each described additive of claim 21-40.

42. according to the described method of claim 41, wherein said personal care formulations or described additive are used for hair treatment.

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