CH713107B1 - Synthetic camel organ extracts, process for their production and their use. - Google Patents

Abstract

Process for the production of synthetic camel organ extracts, the extracts produced with the process according to the invention and their use alone and as a component of cosmetic and dietetic compositions.

Description

Animal organ extracts are of great importance as raw materials for pharmaceuticals and cosmetics. Animal placenta extracts, in particular, are used on a large scale in pharmaceuticals and cosmetics because of the active ingredients they contain [cf. “Seifen-Öle-Fette-Wachse” 112, 211-218 (1986)] Supplements are added.

The frugal camel is becoming increasingly important as livestock, due to a) climate change, which leads to an increase in the desert areas of our earth, b) due to the unregulated growth of our earth's population, whose diet will at some point be questioned if we continue like this as before (e.g. too expensive pig and cattle breeding). The camel family includes: camel (300-950kg) with two humps (Asia), dromedary (300-500kg) with one hump (Africa), lama, alpacka, guanaco, vicuna. The humps are fat reserves.

Since 1947, the inventor has included the camel in his research on animal organ extracts from pigs, cattle, horses, and has also produced natural camel placenta extract (I) and camel thymus extract (II), for example, and their Composition carefully analyzed in order to create the conditions for the development of corresponding protein-free, synthetic extracts I (I, synthetic) and II (II, synthetic). A precursor to I and II was the synthetic injection preparation CELLRYL, Japan (based on calf blood SR 71) developed by the inventor, which was already characterized by cell metabolism-activating properties: Increase in ATP production in the mitochondria, measurable using the WARBURG method Increase factor by 1.8.

The object of the present invention is thus to provide a method for the production of synthetic camel organ extracts, and the resulting synthetic camel organ extracts, which preferably improve the biochemical activity profile of the known natural and synthetic animal organ extracts.

This object is achieved by the present invention. The synthetic camel organ extracts produced by the process according to the invention are distinguished, among other things, by their improved cell metabolism-activating properties and are used in many areas of everyday life, including as additives for cosmetics (for example in skin creams) or as food supplements.

Summary of the invention

To achieve the present object (1) a process for the production of synthetic camel organ extracts is provided, comprising mixing a. of one or more L-amino acids and their derivatives, b. a peptide fraction from a camel organ extract, c. of one or more nucleic acid components and their derivatives, d. and of one or more vitamins and mineral salts,

(2) The method according to item 1, wherein the L-amino acids and their derivatives are selected from the group comprising glycine, glutamic acid, alpha-alanine, aspartic acid, hydroxyproline, proline, serine, lysine, arginine, histidine, ornithine, asparagine , Valine, tyrosine, DL-threonine, phenylalanine, leucine, threonine, tryptophan, methionine, β-alanine, isoleucine, cysteine, creatinine and hippuric acid. (3) Process according to point 1 or 2, whereby in order to preserve the peptide fraction from camel organs, b1) blood-free and washed camel organ tissue is mechanically pulverized and mixed with a suitable solvent or solvent mixture, b2) this mixture is stored in a cool place for several days, and then centrifuged, b3) in the centrifugate by changing the osmotic conditions and simultaneous heating to 55-65 ° C peptides are cleaved from the proteins present, b4) the pH is lowered to 3.2 to 3.5 in order to by sufficiently long Discharge of air or oxygen to remove residual proteins b5) and finally sterile filtering. (4) Process according to one of items 1-3, the suitable solvent mixture being an ether and ethanol and, after step b3), an air mixture to remove the ether at a pH of 7.2 to 7.5 the centrifugate is passed through. (5) The method according to any one of items 1-4, wherein the peptide fraction is obtained from a camel plantenta extract, camel thymus extract or a camel liver extract. (6) Method according to one of items 1-5, wherein the nucleic acid components and their derivatives are selected from the group comprising adenine, adenosine, cytidine, guanine, cytosine, uracil, guanosine, uridine, hypoxanthine, xanthine, cycl. AMP, adenosine monophosphate, inosine, uric acid and orotic acid. (7) Method according to one of items 1-6, wherein the vitamins and mineral salts are selected from the group comprising pyridoxol-HCl, biotin, thiamine chloride, calcium pantothenate, tocopherol succinate, myo-inositol, nicotinamide, magnesium sulfate, magnesium aspartate, sodium dihydrogen phosphate monohydrate, zinc acetate , Cobalt gluconate and manganese gluconate. (8) Method according to one of points 1-7, further additives being selected from the group comprising glucose, sorbitol, mannitol, citric acid, malic acid, succinic acid, benzyl alcohol, glycerine, ethanol, N-methylglucamine, glucosamine, sodium lactate and sodium succinate. (9) Process according to any one of items 1-8, wherein for the production of the synthetic camel organ extracts a. 0.1-50 g / l, 2-10 g / l, or 4 g / l L-amino acids or L-amino acid derivatives, b. the peptide fraction resulting from 0.1-10 kg, from 1-5 kg, or from 2 kg, camel organ tissue per liter of synthetic camel organ extract, c. 0.01-50 g / l, 0.1-5 g / l, or 1.0 g / l nucleic acid components and their derivatives, d. 0.1-1.0 g / l, 0.6-0.9 g / l, or 0.7g / l vitamins and 0.1-20 g / l, 1-5 g / l, or 1.5g / l mineral salts, as well as e. and 0-200 g / l, 5-100 g / l, 80 g / l, 60 g / l or 20 g / l other additives, with 30-40% by volume of water at a pH between 6.0 and 7, 4 to be mixed. (Weight / volume and volume / volume data refer to the synthetic camel organ extract final volume). (10) The method according to any one of items 1-9, wherein for the preparation of the synthetic camel organ extracts a. as L-amino acids or L-amino acid derivatives glycine, glutamic acid each 0.3-0.4 g / l, alpha-alanine, aspartic acid, hydroxyproline, proline, serine, lysine, arginine each 0.2-0.4 g / l, Histidine, ornithine, asparagine, valine, tyrosine, DL-threonine. Phenylalanine, leucine 0.03-0.06 g / l each, threonine, tryptophan, methionine β-alanine 0.01-0.03 g / l each, isoleucine, cysteine, creatinine, hippuric acid <0.02 g / l each , Urea 0.5-0.9 g / l, b. as a peptide fraction, per liter of synthetic camel organ extract, the peptide fraction resulting from 2 kg of camel organ tissue, c. as nucleic acid components and their derivatives, adenine, adenosine, cytidine, guanine, cytosine, uracil, guanosine, uridine, hypoxanthine, xanthine, cycl.AMP, adenosine monophosphate each 0.01-0.03 g / l, inosine 0.08 g / l , Uric acid and orotic acid each 0.02-0.03 g / l, d. as vitamins pyridoxol-HCI 0.5-0.7 g / l, biotin 0.1 g / l, thiamine chloride, calcium pantothenate, tocopherol succinate <0.002 g / l each, myo-inositol nicotine creamid <0.03 g / l each , and as mineral salts magnesium sulfate, magnesium aspartate and sodium dihydrogen phosphate monohydrate each <0.07 g / l, zinc acetate 0.1 g / l, cobalt and manganese gluconate each 0.005 g / l. e. and as further additives glucose, sorbitol and mannitol each 0.1-0.5 g / l, citric acid 1-5 g / l, malic acid 1-2 g / l, succinic acid 5-15 g / l, ethanol 10-50 ml / l, benzyl alcohol 1-4 ml / l., glycerine 0.4-1.0 g / l, N-methylglucamine <0.5g / l, glucosamine 1-2.5 g / l, sodium lactate 1-4 g / l, sodium succinate 2- 15 g / l, mixed with 30-40% by volume of water at a pH between 6.0 and 7.4 (values refer to the final volume). (11) The method according to any one of items 1-10, wherein a yeast cell preparation is also admixed. (12) Method according to one of items 1-11, further components being added which increase the cell metabolic activity-increasing effect of the camel organ extracts and / or have a metabolic activity-increasing effect, selected from the group consisting of carbohydrates and carbohydrate derivatives, such as, for example Glucose, invert sugar, D-mannose, D-ribulose, L-erythrulose-1-phosphate, D-fructose-6-phosphate, DL-arabinose, N-methylhexosamine, aliphatic carboxylic acids with 3 to 6 carbon atoms and buffer substances. (13) Method according to one of items 1-12, further natural or synthetic mammalian organ extracts being admixed with the synthetic camel organ extracts. (14) Method according to one of items 1-13, further natural or synthetic plant extracts being added to the synthetic camel organ extracts. (15) Synthetic camel organ extracts and extract mixtures, as they can be obtained by the method according to one of the items 1-14. (16) The method according to any one of items 1-14, wherein the synthetic camel organ extracts are mixed with one or more camel connective tissue components. (17) Method according to point 16, wherein the camel connective tissue components are to be selected from the group comprising collagen types, elastins, fibronectins, creatines and hyaluronic acid.

Detailed description of the invention

According to a first aspect of the present invention, a method for producing synthetic camel organ extracts is provided. One or more L-amino acids and their derivatives, a peptide fraction from a camel organ extract, one or more nucleic acid components and their derivatives, one or more vitamins and mineral salts and optionally other additives, at 30-40% by volume (volume percent ) Water, preferably double-distilled (double-distilled), dissolved or mixed at a pH between 6.0 and 7.4. The individual components are mixed with stirring and under sterile conditions.

According to the present invention, the group of L-amino acids and L-amino acid derivatives is not restricted further. In one embodiment of the invention, suitable L-amino acids and L-amino acid derivatives are asparagine, aspartic acid, cysteine, cystine, glutamic acid, glutamine, alpha-alanine, beta-alanine, arginine, glycine, histidine, delta-hydroxylysine, hydroxyproline, leucine, isoleucine, Lysine, methionine, norleucine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, alpha-aminoadipic acid, alpha-aminobutyric acid (normal), gamma-amino-n-butyric acid, beta-amino-isobutyric acid, delta-aminolevulinic acid, carbamic acid , Citrulline, creatine, creatinine, cystathionine, cysteic acid, ergothioneine (betaine des thiolhistidine), glycocyamine (guinidine acetic acid), homoserine, ornithine, taurine, djenkolic acid (cysteine thioformacetal), guanidine salts, ornithuric acid, phenacetylic acid alanine, N-acetyl-L-arginine, N-acetylglycine, N-acetyl-L-hydroxyproline, N-acetyl-L-isoasparagine, N-acetyl-L-isoleucine, N-acetyl-L-leucine, N-acetyl- L-lysine, N-acetyl-L-methionine, N-acetylmura minic acid, N-acetyl-L-ornithine, N-acetyl-L-phenylalanine, N-acetyl-L-proline, 0-acetyl-L-serine, N-acetyl-L-threonine, N-acetyl-L-tryptophan, N-acetyl-L-valine, L-allo-isoleucine, L-allo-threonine, N-benzoyl-L-arginine, N-benzoyl-L-histidine, N-benzoyl-L-lysine, N-benzoyl-L- methionine, N-benzoyl-L-ornithine, N-benzoyl-L-phenylalanine, N-benzoyl-L-tryptophan, N-benzoyl-L-valine, S-benzoyl-L-cysteine, O-benzoyl-L-serine, O-benzoyl-L-tyrosine, L-carnosine (β-alanyl-L-histidine), N, O-diacetyl-L-threonine and O-phospho-L-serine.

In an alternative embodiment of the invention, the group of suitable L-amino acids and L-amino acid derivatives consists of glycine, glutamic acid, alpha-alanine, aspartic acid, hydroxyproline, proline, serine, lysine, arginine, histidine, ornithine, asparagine, valine, Tyrosine, DL-threonine, phenylalanine, leucine, threonine, tryptophan, methionine, β-alanine, isoleucine, cysteine, creatinine and hippuric acid.

According to the present invention, a peptide fraction from camel organs is obtained as follows. Blood-free and washed camel organ tissue is mechanically crushed to a pulp and mixed with one or more suitable solvents, kept refrigerated (preferably at <-10 ° C) for several days, e.g. 5, 10 or 15 days, and then centrifuged (e.g. with an explosion-proof drum centrifuge). A suitable solvent mixture is e.g. an ether and ethanol, preferably in a ratio of 13.3: 1. When the osmotic conditions of the centrifugate are changed and the centrifugate is heated to about 60 ° C, peptides are split off from the proteins contained in the centrifugate. Then air can be passed through at a pH of 7.2 to 7.5 to remove the solvent (ether). The pH is then lowered to approx. 3.4 in order to remove residual proteins by passing air or oxygen through them. Free from protein can be determined with sulfosalicylic acid. After sterile filtration (pore size 0.22 µm), the required peptide fraction (flow-through) is obtained.

Under camel organ or camel organ tissue according to the present invention, any organ or part of an organ (organ tissue) originating from a camel is to be understood. In an alternative embodiment of the invention, the camel organ or camel organ tissue is placenta, thymus gland, liver, spleen, heart muscle, connective tissue, or salivary gland. In a preferred embodiment of the invention, the camel organ or camel organ tissue is camel placenta, camel thymus, camel liver, or camel pancreas. In a further preferred embodiment of the invention, the camel organ or camel organ tissue is camel placenta or camel thymus.

The group of nucleic acid components and nucleic acid derivatives according to the present invention is not limited further. Examples of nucleic acid components and their derivatives are 2-aminopurine, hypoxanthine, 1-methylhypoxanthine, adenine, 2-methyladenine, 6-methylaminopurine, guanine, 1-methylguanine, 7-methylguanine, 2-methylamino-6-oxodihydropurine, 8-hydroxyguanine, isoguanine , 2,6-diaminopurine, xanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, 3,9-dimethylxanthine, uric acid, 1-methyluric acid, 9-methyluric acid, 1,9-dimethyluric acid, 3,7-dimethyluric acid, 1 , 3,7-trimethyluric acid, adenosine, 3'-amino-3'-deoxy-adenosine, 9-β-D-ribofuranosyl-6-methylaminopurine, 2'-deoxyinosine, 2'-adenylic acid, 3'-adenylic acid, 5 ' -Adenylic acid, adenosine-5'-diphosphoric acid, adenosine-5'-triphosphoric acid, deoxyadenylic acid, 2'-deoxyadenosine-5'-triphosphate, N-succinyladenylic acid, 3'-guanylic acid, guanosine-5'-diphosphoric acid, guanosine-5'- triphosphoric acid, inosine-3'-phosphoric acid, inosine-5'-phosphoric acid, inosine-5'-diphosphoric acid, xanthylic acid, xanthosine-5'-monophosphoric acid, guanosine-diphosphate mannose, guanosine-diphosphate-fr uctose, guanosine diphosphate fucose, diphosphopyridine nucleotide, triphosphopyridine nuelotide, cytosine, 5-methylcytosine, 5-hydroxymethylcytosine, cytimidine, thymine, uracil, willardiine, 5-aminouracil, 4,5-dihydrouridine, 4,5-dihydrouracil, 4-aminouracil 2'-deoxyuridine, 5-methyluridine, pseudouridine, orotidine, cytidine, 2'-deoxycytidine, 5-methylcytidine, thymidine, α-thymidine, cytidine-2'-monophosphate, cytidine-3'-monophosphate, cytidine-5'-monophosphate , Cytidine-5'-diphosphate, uridine-2'-monophosphate, uridine-3'-monophosphate, orotic acid, uridine-5'-monophosphate, uridine-5'-diphosphate, 5-methylcytidine-3'-monophosphate, orotidine-5 '-monophosphate, thymidine-5'-monophosphate, thymidine-5'-triphosphate, 2'-deoxycytidine-5'-monophosphate, 2'-deoxycytidine-5-diphosphate, uridine-diphosphate-glucose, uridine-diphosphate galactose, uridine-diphosphate-arabinose, uridine-xiphosphate-arabinose -glucuronic acid, uridine diphosphate-N-acetylgalactosamine, cytidine diphosphate-a-glycerine, cytidine diphosphate ribitol, cycl. AMP, cycl. GMP et al.

In a special embodiment of the invention, the group of nucleic acid components and nucleic acid derivatives consists of adenine, adenosine, cytidine, guanine, cytosine, uracil, guanosine, uridine, hypoxanthine, xanthine, cycl AMP, adenosine monophosphate, inosine, uric acid and orotic acid.

The group of vitamins and mineral salts in relation to the present invention includes all known vitamins and mineral salts. A non-exhaustive list of vitamins and mineral salts includes pyridoxol HCI, biotin, thiamine chloride, D-panthenol calcium pantothenate, tocopherol succinate, myo-inositol, nicotinamide, magnesium sulfate, magnesium aspartate, sodium dihydrogen phosphate monohydrate, zinc acetate, cobalt gluconate, and cobalt gluconate.

The group of further additives in relation to the present invention includes substances that improve the effectiveness and effect of the synthetic camel organ extracts, for example by improving the shelf life, the solubility of the individual components, the buffering or effectiveness. The group of other additives includes glucose, sorbitol, mannitol, citric acid, malic acid, succinic acid, benzyl alcohol, glycerine, ethanol, N-methylglucamine, sodium lactate and sodium succinate.

In an alternative embodiment of the method according to the invention, a. 0.1-50 g / l, 2-10 g / l, or 4 g / l L-amino acids or L-amino acid derivatives, b. the peptide fraction resulting from 0.1-10kg, from 1-5kg, or from 2kg, camel organ tissue per liter of synthetic camel organ extract, c. 0.01-50 g / l, 0.1-5 g / l, or 1 g / l nucleic acid components and their derivatives, d. 0.1-1.0 g / l, 0.6-0.9 g / l, or 0.7g / l vitamins and 0.1-20 g / l, 1-5 g / l, or 1.5g / l mineral salts, as well as e. and 0-200 g / l, 5-100 g / l, 80 g / l, 60 g / l or 20 g / l other additives, with 30-40% by volume of water at a pH between 6.0 and 7, 4 mixed (weight / volume and volume / volume data relate to the synthetic camel organ extract final volume).

In a further embodiment of the process according to the invention, a. as L-amino acids or L-amino acid derivatives glycine, glutamic acid each 0.3-0.4 g / l, alpha-alanine, aspartic acid, hydroxyproline, proline, serine, lysine, arginine each 0.2-0.4 g / l, Histidine, ornithine, asparagine, valine, tyrosine, DL-threonine. Phenylalanine, leucine 0.03-0.06 g / l each, threonine, tryptophan, methionine β-alanine 0.01-0.03 g / l each, isoleucine, cysteine, creatinine, hippuric acid <0.02 g / l each , Urea 0.5-0.9 g / l, b. as a peptide fraction, per liter of synthetic camel organ extract, the peptide fraction resulting from 2 kg of camel organ tissue, c. as nucleic acid components and their derivatives, adenine, adenosine, cytidine, guanine, cytosine, uracil, guanosine, uridine, hypoxanthine, xanthine, cycl.AMP, adenosine monophosphate each 0.01-0.03 g / l, inosine 0.08 g / l , Uric acid and orotic acid each 0.02-0.03 g / l, d. as vitamins pyridoxol-HCI 0.5-0.7 g / l, biotin 0.1 g / l, thiamine chloride, calcium pantothenate, tocopherol succinate <0.002 g / l each, myo-inositol nicotine creamid <0.03 g / l each , and as mineral salts magnesium sulfate, magnesium aspartate and sodium dihydrogen phosphate monohydrate each <0.07 g / l, zinc acetate 0.1 g / l, cobalt and manganese gluconate each 0.005 g / l. e. and as further additives glucose, sorbitol and mannitol each 0.1-0.5 g / l, citric acid 1-5 g / l, malic acid 1-2 g / l, succinic acid 5-15 g / l, ethanol 10-50 ml / l, benzyl alcohol 1-4 ml / l., glycerine 0.4-1.0 g / l, N-methylglucamine <0.5g / l, glucosamine 1.0-2.5 g / l, sodium lactate 1-4 g / l, Sodium succinate 2-15 g / l, mixed with 30-40% by volume of water at a pH between 6.0 and 7.4 (values refer to the final volume).

In an alternative embodiment of the invention, a special yeast cell preparation is added in the process for producing the synthetic camel organ extracts, which leads to a further increase in metabolic activity. An example of such a yeast cell preparation is H 38 (“75 Years Chemistry. Re-Reading”, Volume II, pages 448-449 (ISBN 978-1-882292-34-9). In a further alternative embodiment of the method according to the invention, FIG -500 ml / l, 10-150 ml / l or 50 ml / l (based on the synthetic camel organ extracts final volume) H 38 yeast cell preparation added Enhance the properties of camel organ extracts.

In a further alternative embodiment of the invention, in addition to the peptide extract, the amino acids and nucleic acids and their derivatives, the vitamins, mineral salts, and the other additives, further components are added which increase the cell metabolism activity-increasing effect of the camel organ extracts. Components that increase the cell metabolism activity-increasing effect of camel organ extracts include carbohydrates and carbohydrate derivatives, such as glucose, invert sugar, D-mannose, D-ribulose, L-erythrulose-1-phosphate, D-fructose-6-phosphate, D, L-arabinose, N-methylhexosamine, aliphatic carboxylic acids with 3 to 6 carbon atoms and buffer substances.

In a further embodiment of the invention, the synthetic camel organ extracts are admixed with further components which increase metabolic activity. The synthetic camel organ extracts according to the invention are effective substitutes or supplements for a large number of natural product extracts, in particular for natural or synthetic mammalian organ and plant extracts, in the form of placenta, thymus, blood, blood serum, spleen -, liver, heart muscle, elastin, collagen, connective tissue, amniotic fluid, umbilical cord, jellyfish and roe extracts and combinations thereof. The synthetic camel organ extracts according to the invention can be combined with such natural substance extracts, for example natural or synthetic mammalian organ and plant extracts.

Another aspect of the invention relates to the synthetic camel organ extracts produced by the method according to the invention. The composition and quality of the synthetic camel organ extracts can be validated with the aid of analytical methods customary in the state of the art. Analytical parameters that can be tested in this way include pH value, dry residue (5 h at 105 ° C), N content (according to Kjeldahl), amino acid determination: qualitatively using ninhydrin, using thin layer chromatography (TLC) and HPLC; Detection of peptides: using biuret reagent, protein-free (using sulfosalicylic acid); Nucleic acid components. TLC; and sterility tests according to DAB 10.

Table 1. Quality standard of protein-free, synthetic camel placenta extract (I, synthetic)

PH 6.7-7.5 dry substance 0.5-0.8% N 0.05-0.08% amino acids positive peptides (biuret) positive nucleic acid components positive hormones negative heavy metals <20 ppm sterility germ-free pyrogenicity pyrogen-free BSE BSE-free

The synthetic camel organ extracts described above can, individually or in the form of mixtures or combinations thereof with other collagen or other organ extracts from the camel and other organisms for the production of cosmetic formulations, such as cream formulations.

The synthetic camel organ extracts can also be used individually or in the form of mixtures or combinations, undiluted or diluted, in the form of capsules containing them for direct oral intake, for example as food supplements or food enhancers.

Feed supplement for camels by synthetic camel organ extract application We found: a) Corresponding applications on young animals of camels that did not want to eat enough: bad eater, showed success some time after such a treatment. It even had to be given soon be discontinued from such extracts, since the bad eatern became over eater, b) The camel organ extracts were suitable as food supplements for camels that were convalescent c) Use of camel organ extracts as injection preparations: Appropriate experiments were possible through mediation of the Hamburg friend Wolfgang Seidel, and also of the former rector of the UFSM, Santa Maria Professor Dr. Jose Mariano da Rocha Filho, with the camels of her sheik friend: The sheik has acquired the camel thymus extract as an injectable solution, which is produced according to the inventor's recipe, for several years since his camels had positive racing successes.

Just as the fermentation of normal baker's yeast can be increased by adding the metabolism-activating yeast preparations H 38 developed by the author, the activity of the camel organism can also be increased by applying metabolic-activating camel organ extracts. This is of interest for racing camels or for camels in convalescence.

The use of the synthetic camel organ extracts produced according to the invention, individually or in the form of mixtures or combinations thereof with other organ extracts, is not restricted to certain living beings. In one embodiment of the invention, the synthetic camel organ extracts according to the invention are applied to mammals; for example humans, camels, horses, cattle, sheep, chickens or pigs. In a further embodiment, the synthetic camel organ extracts according to the invention are applied to microorganisms or plants.

Production of cream formulations (water / oil emulsions): Since 1965 the inventor has realized the successful use of several protein-free (and enzyme-free) cosmetic additives, such as OMNITHYMUS, k-PFE + CELLRYL in EVANGYL (POLA).

The justification for the use of protein-free organ extracts in cosmetic additives was given by the inventor in “75 Years Chemistry: Re-Reading”, Volume II, page 320 (ISBN 978-1-882292-34-9), also there on page 524 there is a comparison of normal, synthetic and cell culture extracts.

According to the following composition, hand creams were formulated to which synthetic extract I was added. The final composition of the cream formulations was as follows:

Table 2

Soluble lanolin derivative 1.0 acetylated lanolin alcohols 5.0 liquid Multisterol extract 5.0 Vaseline 5.0 polyvinyl alcohol gel 0.75 10% NaOH 2.25 test substances I (I, synthetic) 0.5 ethoxylated fatty amines 3, 75 Perfume oil 0.3 water, double-distilled to 100% by weight (double-distilled: double-distilled)

To produce these cream formulations, the fat phase components and petroleum jelly were heated to approx. 75 ° C. At the same time, the PVA gel was dispersed in hot water at approx. 80 ° C. and gradually dissolved. The ethoxylated fatty amine with approx. 25 EO units and, if necessary, an emulsifier was added to the latter dispersion, after which the previously prepared heated fat phase was emulsified in it. After stirring for 5 minutes at the mixture temperature, the mixture was cooled to 60.degree. C., then 10% sodium hydroxide solution was added and the mixture was cooled further to below 40.degree. The formulations of the test solutions (synthetic extract I) were then added with slow stirring. The batch was homogenized well. The hand cream was packaged in a stable manner and showed improved skin conditioning compared with a batch without the addition of test substance.

Camel connective tissue components (camel collagens)

Manufacturing:

Camel skin was, after removing blood, meat and fat, crushed and then, depending on the objective, subjected to extraction with neutral salts or acids, stored in a freeze-dried state or as a solution (sorbic acid addition for preservation) (see Dtsch.Apotheker-Ztg .117: 1557-62 (1977)). Similar methods are described in the prior art for obtaining other mammalian collagens. Camel collagen: N 18% + - 0.3 Hydroxyproline content: 14-15%.

According to an alternative embodiment of the invention, the synthetic camel organ extracts produced by the process according to the invention are mixed with one or more camel connective tissue components. Examples of such connective tissue components are collagen types, elastins, fibronectins, creatines and hyaluronic acid.

Examples

Example 1

Production of synthetic camel placenta extract (I, synthetic) as a 1 liter batch The following components, calculated to a final volume of 1 l, were in a volume of double distilled with stirring and under sterile conditions. H20 dissolved, which corresponds to 3/10 to 4/10 of the final volume. The pH value was kept between 6.0 and 7.4: L-amino acids and derivatives: glycine, glutamic acid each 0.3-0.4g, a-alanine, aspartic acid, hydroxyproline, proline, serine, lysine, arginine each 0 , 2-0.4g, histidine, ornithine, asparagine, valine, tyrosine, DL-threonine. Phenylalanine, leucine 0.03-0.06g each, threonine, tryptophan, methionine β-alanine 0.01-0.03g each, isoleucine, cysteine, creatinine, hippuric acid <0.02g each, urea 0.5-0.9g . Peptide fraction from camel's placental glands: 10kg of thawed, blood-free washed glands comminuted in a tooth colloid mill (up to 0.3 mm pulp) were stored in 4 L ether + 300 ml ethanol in a cold room below -10 ° C for ten days, then in an explosion-proof drum centrifuge Cut. By changing the osmotic conditions of the centrifugate (+ 150gNaCl) and simultaneous heating to 60 ° C, the proteins contained in the centrifugate split off peptides; then air was passed through at pH 7.5 to remove the ether, and - after changing the pH to 3.4 with succinic acid - to remove residual proteins [pass air or oxygen through for a long enough time: test with sulfosalicylic acid]. After Millipore filtration (pore size 0.22 micrometers), the peptide fraction required in the example was obtained (flow-through), the amount obtained from 10 kg of camel placenta glands being sufficient for 5 L of synthetic I-extract, i.e. only 1/5 is used in the example. Nucleic acid components and derivatives: adenine, adenosine, cytidine, guanine, cytosine, uracil, guanosine, uridine, hypoxanthine, xanthine, cycl.AMP, adenosine monophosphate each 0.01-0.03g, inosine 0.08g, uric acid, orotic acid 0.02- 0.03g. Vitamins: Pyridoxol-HCI 0.5-0.7g, biotin 0.1g, thiamine chloride, calcium pantothenate, tocopherol succinate <0.002g each, myo-inositol nicotine creamid <0.03g each. Mineral salts: Mg sulfate, Mg aspartate, NaH2PO4-H2O each <0.07g, Zn acetate: 0.1g, Co-, Mn-gluconate each 0.005g. Further additives: glucose, sorbitol, mannitol each 0.1-0.5g, citric acid 1-5g, malic acid 1-2g, succinic acid 5-15g, ethanol 10-50ml, benzyl alcohol 1-4ml., Glycerine 0.4-1.0g / l , N-methylglucamine <0.5g, nalactate 1-4g, Na-succinate 2-15g, sodium chloride 1-10g, preservation if necessary without.

When preparing the solution, it should be noted that some components must be pre-dissolved and neutralized in NaOH or HCl, for example the amino acids Glu, Asp, Val, Tyr, Phe, Isoleu, Leu in 2N NaOH, xanthine in 3N NaOH, guanine in 3N HCl.

After filling up with redist. Water to 11 final volumes, the pH was adjusted again and sterile-filtered.

Example 2

Synthetic extract I plus H 38 (later Y 20): The production process was carried out as indicated for Example 1. Before making up to 1 l, 50 ml of H 38 (Y 20), a special yeast cell preparation, were added and mixed with redist. Water topped up to 11

The measured respiratory increase factor was 2.6.

Example 3

PADBERG method: The Padberg test evaluates the absorption of methylene blue into the skin, the amount of absorption being related to the dryness of the skin. This is based on the observation that the absorption of methylene blue applied to the skin is proportional to the dryness of the skin; so that the higher the roughness, the more methylene blue is absorbed.

For the implementation of the Padberg test usually 5-15 test persons aged 35-65 years are used. The test subjects are instructed not to use cosmetics on the skin areas that are examined for the test 3 days before the start of the test and during the test. For each test person, 6 test fields are marked on the inner sides of the forearm, whereby one of the test fields remains untreated. First of all, a Padberg test is carried out for each test field to determine the blank value. The test subjects then apply the substance to be tested to the test sites twice a day, in the morning and in the evening, over a period of 14 days. Another Padberg test is carried out four hours after the last application.

The Padberg test is carried out as for example in Journ. Soc. Cosmetic Chemicals 20, 719-728, [1969].

Results of the PADBERG tests: Table 3

In Table 3 below, the photometer values for a cream composition with or without the addition of synthetic extract I (placebo cream), expressed as a percentage of the value for treated skin and based on the initial value, are summarized. Table 3 also shows the calculated mean values.

The results show that the cream formulations with the addition of synthetic extract I can be used to smooth the skin. The same has also been shown when a synthetic thymus extract is used as an additive.

Table 3: Roughness test according to the PADBERG method with cream formulations (water / oil emulsions) with and without the addition of synthetic camel placenta extract (synthetic extract I) (duration of use 14 days). 44 81.0 60.1 43 91.0 64.8 50 80.0 40.0 56 76.5 41.2 51 75.3 70.0 40 95.5 40.4 65 89.0 68.0 66 97.6 55.0 62 92.6 60.0 44 77.0 62.8 mean values 85.6 56.4

Comparative example 4

WARBURG method - trial

In a WARBURG apparatus, the metabolic activity of products was determined with the help of manometric measurements, and in the case of the use of liver homogenate from rats or guinea pigs, the increase in breathing - determined by measuring the O2 uptake. According to the filling plan, the side bulbs of the vessels were filled with test solution or water. The course of the reaction can be compared with and without the addition of product. The vessels were connected to the manometers. The pressure gauges were hung in the thermostat and heated up to the target temperature. Then test solution or water was introduced from the side vessel into the main vessel by tilting. This is the actual start of the reaction. The individual readings were taken at intervals of 10 minutes, the results are entered in the measurement plan. The Warburg experiment lasts 90 minutes. The results provide the factors for increasing metabolic activity.

Measurement plan

Example: rye extract with a factor of 1.83. Control: 1.0 (pre-incubation 1 h at 37 ° C):

Table 4

[0049]

Table 5

Rye extract 1.83 control 1.0 pig placenta extract 1.85 bovine placenta extract 1.8

Example 5

In a manner analogous to Comparative Example 4, respiratory increase factors were measured for synthetic extract I, synthetic extract II, synthetic camel liver extract and for camel NGF (Nerve Growth Promoting Factor).

Table 6

Synthetic extract I 2.3 synthetic extract II 2.2-2.5 synthetic camel liver extract 2.3-2.4 camel NGF 2.4

During the BSE crisis, for example, camel organ extracts were able to replace the undesirable bovine placenta extracts: countries in which camels grow up were guaranteed to be BSE-free. The above value for camel NGF is significant: In clinical trials in Japan, camel NGF has shown positive results in the treatment of facial paralsis. Earlier results with NGF preparations from other animals have been reported in the author's “Re-Reading”, Volume II, page 295: ISBN 978-1-882292-34-9. The extraction of camel NGF was carried out according to the method described there for other mammals: Analysis data for Ve) that regulation for camel NGF: Yield: 1.8 g per kg of camel's salivary gland, with a biological activity in BU of 0.010-0.019 µg /G. Carrying out the biological identification and evaluation of camel NGF according to information such as those made by Montalcini, Cancer Research 14, 49ff (1954) for the extraction and characterization of mouse NGF. With the extraction of camel NGF from a camel's salivary gland, literature references were simultaneously refuted that there are no NGF factors in the salivary glands of large animals (collaboration with Prof. Dr. T. Yamamoto).

By combination, for example with H 38 (later Y 20), a special yeast cell preparation (75 Years Chemistry Re-Reading, Volume II (ISBN 978-1-828292), pages 448-449 and ref [11,14] ), the activity of this extract can be further increased.

Example 6

The following are the results of activity tests for synthetic extract I and, for comparison, pig placenta extract, as well as information on the working methods used:

Table 7

PADBERG test (as above) 56.4 (85.6) 64.5 (88.9) metamorphosis onset shortening Xenopus <1> 5-8 days 3-4 days wound healing (skin tension) <2> 240 235 <1> Description of the methodology in "Re-Reading", Part IV, p 448-456: IBSN 978-1-882292-36-3 <2> Methodology in "Cosmetics and Toiletries" 92, 25-27 (1977)

Beginning of metamorphosis in the camel placenta synthetic I 5-8 days before the control, whereas in the pig placenta synthetic I 3-4 days before the control (see Table 7).

Information on the sources of genetic resources according to Art. 49a PatG Camel organ extract not known Yeast cell preparations not known Mammalian organ extract not known

Claims (8)

A process for the preparation of synthetic camel organ extracts comprising mixing a. one or more L-amino acid (s) and / or derivatives thereof, b. a peptide fraction from a camel organ extract, c. one or more nucleic acid components and / or derivatives thereof, d. and one or more vitamins and mineral salts, with 30-40% by volume of water at a pH between 6.0 and 7.4. 2. The method according to claim 1, wherein i) the L-amino acids and their derivatives are selected from the group comprising glycine, glutamic acid, alpha-alanine, aspartic acid, hydroxyproline, proline, serine, lysine, arginine, histidine, ornithine, asparagine, valine, tyrosine, DL-threonine, phenylalanine , Leucine, threonine, tryptophan, methionine, β-alanine, isoleucine, cysteine, creatinine and hippuric acid. ii) the nucleic acid components and their derivatives are selected from the group comprising adenine, adenosine, cytidine, guanine, cytosine, uracil, guanosine, uridine, hypoxanthine, xanthine, cycl. AMP, adenosine monophosphate, inosine, uric acid and orotic acid iii) the vitamins and mineral salts are selected from the group comprising pyridoxol-HCl, biotin, thiamine chloride, calcium pantothenate, tocopherol succinate, myo-inositol, nicotinamide, magnesium sulfate, magnesium aspartate, sodium dihydrogen phosphate monohydrate, zinc acetate, cobalt gluconate and manganese gluconate iv) further additives are selected from the group comprising glucose, sorbitol, mannitol, citric acid, malic acid, succinic acid, benzyl alcohol, glycerol, ethanol, N-methylglucamine, sodium lactate and sodium succinate. 3. The method according to claim 1 or 2, wherein to preserve the peptide fraction from camel organs b1) blood-free and washed camel organ tissue is mechanically crushed to a pulp and mixed with one or more suitable solvent (s) or solvent mixture, b2) this mixture is stored refrigerated for several days and then centrifuged, b3) the peptides contained in the centrifugate are split off in the centrifugate by changing the osmotic conditions and simultaneous heating to 55-65 ° C, b4) the pH is lowered to 3.2 to 3.5 in order to remove the remaining proteins b5) and finally sterile filtering. 4. The method according to any one of claims 1-3, wherein a yeast cell preparation is also added. 5. The method according to any one of claims 1-4, wherein further components are added which increase the cell metabolic activity-increasing effect of the camel organ extracts and / or have their own metabolic activity-increasing effect selected from the group consisting of carbohydrates and carbohydrate derivatives, such as glucose, Invert sugar, D-mannose, D-ribulose, L-erythrulose-1-phosphate, D-fructose-6-phosphate, D, L-arabinose, N-methylhexosamine, aliphatic carboxylic acids with 3 to 6 carbon atoms and buffer substances. 6. The method according to any one of claims 1-5, wherein the synthetic camel organ extracts further natural or synthetic mammalian organ extracts are added. 7. The method according to any one of claims 1-6, wherein the synthetic camel organ extracts are mixed with one or more camel connective tissue components selected from the group comprising collagen types, elastins, fibronectins, creatines and hyaluronic acid. 8. Synthetic camel organ extracts obtained by the method according to any one of claims 1-6.