CH396925A - Process for the preparation of new pyrazolopyrimidines - Google Patents

Process for the preparation of new pyrazolopyrimidines

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Publication number
CH396925A
CH396925A CH1311064A CH1311064A CH396925A CH 396925 A CH396925 A CH 396925A CH 1311064 A CH1311064 A CH 1311064A CH 1311064 A CH1311064 A CH 1311064A CH 396925 A CH396925 A CH 396925A
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Switzerland
Prior art keywords
radical
lower alkyl
alkyl radical
groups
hydrogen atom
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CH1311064A
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German (de)
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Paul Dr Schmidt
Kurt Dr Eichenberger
Max Dr Wilhelm
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Ciba Geigy
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Priority to CH1311064A priority Critical patent/CH396925A/en
Publication of CH396925A publication Critical patent/CH396925A/en

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D231/00Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/10Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D231/14Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D231/38Nitrogen atoms
    • C07D231/40Acylated on said nitrogen atom

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)

Description

  

  
 



  Verfahren zur Herstellung neuer   Pyrazolopyrimidine   
Gegenstand der Erfindung ist ein Verfahren zur Herstellung von   Pyrazolo[3,4-djpyrimidinen    der Formel
EMI1.1     
 worin R6 einen Aralkyl- oder Heterocyclylalkylrest oder einen Alkylrest mit mehr als 2 Kohlenstoffatomen bedeutet, R3 für ein Wasserstoffatom oder einen niederen Alkylrest steht und   Rt    ein Wasserstoffatom, einen Alkylrest, einen Hydroxyalkylrest, einen Halogenalkylrest, einen Oxaalkyl-, Cycloalkyloder Cycloalkylalkylrest oder einen Aralkyl oder Heterocyclylalkylrest oder einen höchstens zweikernigen Aryl- oder heterocyclischen Rest bedeutet, oder ihrer Salze.



   In den neuen Verbindungen kommen als Alkylreste insbesondere niedere Alkylreste, wie Methyl-,   Sithyl-,    Propyl-, Isopropyl-, Butyl-, Isobutyl-, Pentyl-(l)-, Pentyl-(2)-, Pentyl-(3)-, 2-Methylbutyl (3)- oder Hexylreste, als Halogenalkylreste oder Hydroxyalkylreste beispielsweise Chloräthyl oder Hydroxyäthylreste und als Oxa-alkylreste z. B. 3-Oxa  pentyl-(5)-oder    3-Oxa-heptyl-(6)-reste in Betracht.



   Cycloalkyl- oder Cycloalkylalkylreste sind beispielsweise Cyclopentyl- oder Cyclohexylreste, oder Cyclopentyl- oder   Cyclohexyl-methyl-, -äthyl- oder    -propylreste.



   Als Aralkyl- oder Arylreste kommen insbesondere Phenylalkylreste, wie 1- oder 2-Phenyläthyloder Phenylmethylreste, oder Phenylreste in Frage, in denen die aromatischen   Kerne    Substituenten tragen können, wie niedere Alkylreste oder freie oder substituierte Hydroxy-,   Amino-oder    Mercaptogruppen, Halogenatome,   Trifluormethyl- oder    Nitrogruppen. In den genannten substituierten Hydroxy-, Mercapto- oder Aminogruppen sind die Substituenten insbesondere solche der obengenannten Art, vor allem niedere Alkylreste, so dass es sich z.

   B. um Methoxy-,   l2ithoxy-,    Propoxy- oder Butoxygruppen, entsprechende Alkylmercaptogruppen, Alkylendioxygruppen, wie Methylendioxygruppen, Mono- oder Dialkylaminogruppen, wie   Mono-oder    Dimethyl-,    -äthyl-, -propyl-, -butyl- oder -pentylaminogruppen    handelt. Als Halogenatome sind vor allem Fluor-, Chlor- oder Bromatome zu nennen. Die Aralkylreste können mehrere Arylreste enthalten, wie z. B. im Diphenylmethylrest.



   Als heterocyclische Reste bzw. Heterocyclylalkylreste seien beispielsweise Pyridyl-, Thienyl-, Furyl-, Thenyl- oder Furfurylreste, die im heterocyclischen Rest wie oben für die Arylreste angegeben substituiert sein können, genannt.



   Die neuen Verbindungen und ihre Salze besitzen wertvolle pharmakologische Eigenschaften. Insbesondere sind sie coronarerweiternd wirksam. Die neuen   Verbindungen können somit als Heilmittel, insbesondere bei Durchblutungsstörungen des Herzmuskels, aber auch als Zwischenprodukte zur Herstellung solcher Heilmittel dienen.



   Besonders wertvoll als coronarerweiternde Mittel sind Verbindungen der Formel
EMI2.1     
 und ihre tautomeren Formen und die Salze davon, worin   Rt    ein Wasserstoffatom, einen Niederalkylrest, z. B. Methyl, Äthyl, Propyl, Isopropyl, Butyl-(2), 3-Methyl-butyl-(2), Pentyl-(2) Pentyl-(3), einem Cycloalkylrest, z. B.

   Cyclopentyl oder Cyclohexyl, einen Hydroxyniederalkylrest, wie Hydroxyäthyl einen Halogenniederalkylrest, wie Chloräthyl, einen Oxaniederalkylrest, wie 3-Oxapentyl, oder einen   Arykest,    wie Phenylrest, bedeutet, wobei die Arylreste unsubstituiert oder durch Halogenatome, wie Chlor oder Brom, niedere Alkoxygruppen, wie Methoxy oder Äthoxy, niedere Alkylreste, wie Methyl, Äthyl, Propyl, Isopropyl, Butyl- tert.-Butyl, Methylendioxygruppen oder Trifluormethylgruppen, mono-, die oder trisubstituiert sein können, oder einen Pyridylrest darstellt, R3 Wasserstoff oder Niederalkyl ist und   R6    für einen Aralkyl-, wie einen Phenylniederalkyl-, vor allem Phenylmethylrest, steht, wobei die Arylreste, wie eben gezeigt, substituiert sein können.



   Ferner sind von Bedeutung die Verbindungen der Formel
EMI2.2     
 und ihre tautomeren Formen und die Salze davon, worin R1 einen Niederalkylrest, z. B. Methyl, Äthyl, Propyl, Isopropyl, Butyl-(2), 3-Methyl-butyl-(2), Pentyl-(2), Pentyl-(3), einen Cycloalkylrest, z. B.



  Cyclopentyl oder Cyclohexyl, einen Halogenniederalkylrest, wie Chloräthyl, einen Oxaniederalkylrest, wie 3-Oxapentyl, bedeutet und   R2    für Wasserstoff oder Niederalkyl steht und   R6    einen Alkylrest mit mehr als 2 Kohlenstoffatomen darstellt, z. B. Propyl, Isopropyl, Butyl, Isobutyl, Amyl oder Isoamyl bedeutet.



   Besonders wertvoll sind die Verbindungen der Formel
EMI2.3     
 und ihre tautomeren Formen und Salze davon, worin   Rt    einen niederen Alkylrest darstellt,   R5    einen niederen Alkylrest oder vor allem Wasserstoff und   R6    einen unsubstituierten oder im Phenylrest durch Chloratome, Methoxygruppen, Methylendioxygruppen, Methylgruppen oder Trifluormethylgruppen mono-, die oder trisubstituierten Benzylrest darstellt.



   Zu nennen sind besonders das 1 -Isopropyl-4-hydroxy-6-benzyl-pyrazolo   [3 ,4-d]pyrimidin,    das   1 -Isopropyl-4-hydroxy-6-(p-chlorbenzyl) -    pyrazolo[3   4-dipyrimidin,    das   l-Isopropyl-4-hydroxy-6-(m-methoxybenzyl)-       pyrazolo[3 4-d] pyrimidin,    das   l-IsopropylXhydroxy-6-(3', 4', 5'-trimethoxy-       phenylmethyl)-pyrazolo [3      ,4-djpyrimidin    und das   1 -Pentyl-(3 )-4hydroxy-6-benzyl-pyrazolo-       [3,4a]pyrimidin    und ihre Salze.



   Das erfindungsgemässe Verfahren zur Herstellung der neuen Verbindungen besteht darin, dass man in einem Nitril der Formel
EMI2.4     
 die Nitrilgruppe durch Hydrolyse in die Carbamylgruppe überführt, z. B. durch Behandlung mit Alkalien in Gegenwart von Oxydationsmitteln, wie Wasserstoffsuperoxyd, und das erhaltene Produkt cyclisiert. Dabei kann der Ringschluss gleichzeitig mit der Umwandlung in die Carbamylgruppe erfolgen.



   Die erhaltenen 4-Hydroxy-pyrazolopyrimidine können in üblicher Weise in ihre Salze mit Basen, z. B. in ihre Metallsalze, wie Alkalimetallsalze, umgewandelt werden, z. B. durch Lösen in Alkalilaugen. Die Salze ihrerseits lassen sich in die freien Hydroxyverbindungen umwandeln, zweckmässig durch Behandlung mit Säuren.



   Die neuen, pharmakologisch wertvollen Verbindungen, ihre Salze oder entsprechende Gemische können z. B. in Form pharmazeutischer Präparate Verwendung finden. Diese enthalten die genannten Verbindungen in Mischung mit einem für die enterale oder parenterale Applikation geeigneten pharmazeutischen organischen oder anorganischen Trägermaterial.  



   Die verfahrensgemäss erhaltenen Endstoffe sind aber auch wertvolle Zwischenprodukte, z. B. für die Herstellung der in den Schweizer Patenten Nrn. 390264 und 390 929 beschriebenen   4-Mer-    capto- oder   4-Amihoverbindungen.   



   Sofern die beim erfindungsgemässen Verfahren verwendeten Ausgangsstoffe neu sind, lassen sie sich nach an sich bekannten Methoden herstellen.



   Als Ausgangsstoffe werden gemäss der vorliegenden Erfindung vorzugsweise diejenigen verwendet, die zu den eingangs als besonders wertvoll geschilderten Endstoffen führen.



   Im nachfolgenden Beispiel sind die Temperaturen in Celsiusgraden angegeben.



   Beispiel
7,95 g 2-Isopropyl-3-(p-chlorphenylacetylamino)4-pyrazol-carbonsäure-nitril werden mit 27,2 cm3   10% iger    Kalilauge und 102   cms      3 % igem    Wasserstoffsuperoxyd während 10 Stunden auf 700 erwärmt.



  Anschliessend filtriert man die Reaktionslösung und säuert mit 2n Salzsäure auf pH 5 an, worauf sich das 1-Isopropyl-4-hydroxy-6-(p-chlorbenzyl) pyrazolo[3,4-d]pyrimidin der Formel
EMI3.1     
 in farblosen Kristallen vom F. 181-182  ausscheidet.



   Das als Ausgangsmaterial verwendete 2-Isopropyl-3-(p-chlorphenylacetylamino)-4-pyrazol-carbonsäure-nitril kann wie folgt hergestellt werden:
Zu 45,5 g   2-Isopropyl-3-amino-4-cyano-pyrazol    in 325 cm3 abs. Dioxan und 24   cm3    Pyridin gibt man tropfenweise unter Rühren eine Lösung von 55,8 g p-Chlorphenylessigsäurechlorid in 75 cm3 Dioxan bei einer Temperatur zwischen   10-150.    Nach dem Zutropfen rührt man noch eine Stunde bei 100 und dann noch 2 Stunden bei Raumtemperatur.

   Nach Zugabe von 100 cm3 Wasser und 200 cm3 2n Salzsäure kristallisiert das   2-Isopropyl-3-(p-chlorphenyl-    acetylamino)-4-pyrazolcarbonsäurenitril der Formel
EMI3.2     

In analoger Weise kann man die folgenden Verbindungen erhalten: a) 1-Isopropyl-4-hydroxy-6-benzyl-pyrazolo   [3 4-dipyrimidin,   
F. 165-166  (aus Alkohol). b) 1-Methyl-4-hydroxy-6-benzyl-pyrazolo  [3,4-d]pyrimidin,
F. 236-237  (aus Äthanol). c) 1-Methyl-4-hydroxy-6-(3',4',5'-trimethoxy phenyl-methyl)-pyrazolo[3,4-d]pyrimidin,
F. 2450 (aus Chloroform-Petroläther). d) 1-Isopropyl-4-hydroxy-6-(3',4',5'-trimethoxy phenyl-methyl)-pyrazolo[3,4-d]pyrimidin,
F. 195-196  (aus Alkohol). e) 1-Isopropyl-4-hydroxy-6-p-äthoxybenzyl pyrazolo[3,4-d]pyrimidin,
F. 175-176  (aus Alkohol). f) 1-sek.-Butyl-4-hydroxy-6-benzyl-pyrazolo  [3,4-d]pyrimidin,
F.

     15P1550    (aus Alkohol). g) 1-Cyclohexyl-4-hydroxy-6-benzyl-pyrazolo   [3 ,4-d]pyrimidin,   
F. 207-208  (aus Alkohol). h) 1-(3'-Pentyl)-4-hydroxy-6-benzyl-pyrazolo   [3 4-dipyrimidin,   
F 144-145  (aus abs. Alkohol). i) 1-Cyclopentyl-4-hydroxy-6-benzyl-pyrazolo  [3,4-d]pyrimidin,
F. 189-190  (aus abs.

   Alkohol). k) 1-(ss-Hydroxy-äthyl)-4-hydroxy-6-benzyl pyrazolo[3,4-d]pyrimidin,
F.   19P1950    (aus Alkohol). l) 1-Isopropyl-4-hydroxy-6-(m-methoxy-benzyl) pyrazolo[3,4-d]pyrimidin,
F. 155-158  (aus Alkohol). m) 1-[1'-Äthoxy-butyl-(3')]-4-hydroxy-6-benzyl pyrazolo[3,4-d]pyrimidin,
F. 111-112  (aus Methanol-Wasser). n)   l-Methyl-4-hydroxy-6-p-chlorbenzyl-pyrazolo-       [3 4-dipyrimidin,   
F. 268-270  (aus Dimethylformamid-Wasser). o) 1-Methyl-4-hydroxy-6-(2',3'-dimethoxy phenyl-methyl)-pyrazolo[3,4-d]pyrimidin,
F. 190-191  (aus Alkohol). p) 1-Phenyl-4-hydroxy-6-benzyl-pyrazolo   [3 ,4-d]pyrimidin,   
F. 264-265  (aus Chloroform-Petroläther). q) 1-Phenyl-4-hydroxy-6-(m-methoxybenzyl) pyrazolo[3,4-d]pyrimidin,
F.

   2350 (aus   Chloroform-Petroläther).    r)   1-α-Pyridyl-4-hydroxy-6-benzyl-pyrazolo-     [3,4-d]pyrimidin,
F.   ?      3600    (aus Dimethylformamid). s)   4-Hydroxy-6-benzyl-pyrazolo [3    ,4-d]pyrimidin,
F. 290-292  (aus Äthanol). t) 1-Isopropyl-4-hydroxy-6-(o-methoxy-benzyl) pyrazolo[3,4-d]pyrimidin,
F. 157-159  (aus Äthanol). u) 1-Isopropyl-4-hydroxy-6-(2'-methyl-3'-methoxy phenyl-methyl)-pyrazolo[3,4-d]pyrimidin,
F. 150-151  (aus   Athanol).     v)   l-Isopropyl-Phydroxy-6-diphenyImethyl-       pyrazolo[3,4-d]pyrimidin,   
F. 226-227  (aus Äthanol). w) 1-[3'-Methyl-butyl-(2')]-4-hydroxy-6-benzyl pyrazolo[3,4-d]pyrimidin,
F. 157-158  (aus Äthanol).    x) 1-Isopropyl-4-hydroxy-6-(α

  -phenyl-propyl)-    pyrazolo[3,4-d]pyrimidin,
F. 142-143  (aus Alkohol). y) 1-Isopropyl-4-hydroxy-6-(ss-phenyl-äthyl) pyrazolo[3,4-d]pyrimidin,
F.   12P1250    (aus Alkohol). z) 1-Isopropyl-4-hydroxy-6-(m-hydroxy-benzyl) pyrazolo[3,4-d]pyrimidin,
F. 226-227  (aus Alkohol). aa) 1-Isopropyl-4-hydroxy-6-isopropyl-pyrazolo   [3,4-dipyrimidin,   
F. 175-177  (aus Äthanol). bb) 1-Isopropyl-4-hydroxy-6-(2'-methyl-propyl) pyrazolo [3 ,4-d]pyrimidin,
F.   11P1160    (aus Äthanol).    cc) 1 -sek.-Butyl-4-hydroxy-6-isopropyl-pyrazolo-     [3   4-dipyrimidin,   
F. 146-148  (aus Ather-Petroläther). dd)   l-sek.-Butyl-Phydroxy-6-(2'-methyl-propy    pyrazolo[3,4-d]pyrimidin,
F.   115-116     (aus Äther-Petroläther).   



  
 



  Process for the preparation of new pyrazolopyrimidines
The invention relates to a process for the preparation of pyrazolo [3,4-djpyrimidines of the formula
EMI1.1
 where R6 is an aralkyl or heterocyclylalkyl radical or an alkyl radical with more than 2 carbon atoms, R3 is a hydrogen atom or a lower alkyl radical and Rt is a hydrogen atom, an alkyl radical, a hydroxyalkyl radical, a haloalkyl radical, an oxaalkyl, cycloalkyl or cycloalkylalkyl radical or an aralkyl or Heterocyclylalkyl radical or an at most binuclear aryl or heterocyclic radical, or their salts.



   In the new compounds, lower alkyl radicals such as methyl, sithyl, propyl, isopropyl, butyl, isobutyl, pentyl (1), pentyl (2), pentyl (3) - , 2-methylbutyl (3) - or hexyl radicals, as haloalkyl radicals or hydroxyalkyl radicals, for example chloroethyl or hydroxyethyl radicals and as oxa-alkyl radicals z. B. 3-Oxa pentyl (5) or 3-oxa-heptyl (6) radicals into consideration.



   Cycloalkyl or cycloalkylalkyl radicals are, for example, cyclopentyl or cyclohexyl radicals, or cyclopentyl or cyclohexyl-methyl, -ethyl or -propyl radicals.



   Particularly suitable aralkyl or aryl radicals are phenylalkyl radicals, such as 1- or 2-phenylethyl or phenylmethyl radicals, or phenyl radicals in which the aromatic nuclei can carry substituents, such as lower alkyl radicals or free or substituted hydroxy, amino or mercapto groups, halogen atoms, trifluoromethyl - or nitro groups. In the substituted hydroxy, mercapto or amino groups mentioned, the substituents are in particular those of the type mentioned above, especially lower alkyl radicals, so that there are, for.

   B. methoxy, l2ithoxy, propoxy or butoxy groups, corresponding alkyl mercapto groups, alkylenedioxy groups such as methylenedioxy groups, mono- or dialkylamino groups such as mono- or dimethyl, -ethyl, -propyl, -butyl or -pentylamino groups. Particularly fluorine, chlorine or bromine atoms should be mentioned as halogen atoms. The aralkyl radicals can contain several aryl radicals, such as. B. in the diphenylmethyl radical.



   Examples of heterocyclic radicals or heterocyclylalkyl radicals that may be mentioned are pyridyl, thienyl, furyl, thenyl or furfuryl radicals, which can be substituted in the heterocyclic radical as indicated above for the aryl radicals.



   The new compounds and their salts have valuable pharmacological properties. In particular, they are effective in expanding the coronary artery. The new compounds can thus serve as remedies, in particular for circulatory disorders of the heart muscle, but also as intermediate products for the production of such remedies.



   Compounds of the formula are particularly valuable as coronary expanding agents
EMI2.1
 and their tautomeric forms and the salts thereof, wherein Rt is hydrogen, lower alkyl, e.g. B. methyl, ethyl, propyl, isopropyl, butyl- (2), 3-methyl-butyl- (2), pentyl- (2) pentyl- (3), a cycloalkyl radical, e.g. B.

   Cyclopentyl or cyclohexyl, a hydroxy lower alkyl radical such as hydroxyethyl, a halo-lower alkyl radical such as chloroethyl, an oxane lower alkyl radical such as 3-oxapentyl, or an aryl radical such as phenyl radical, the aryl radicals being unsubstituted or by halogen atoms, such as chlorine or bromine, lower alkoxy groups such as methoxy or ethoxy, lower alkyl radicals such as methyl, ethyl, propyl, isopropyl, butyl tert-butyl, methylenedioxy groups or trifluoromethyl groups, mono-, which can be or trisubstituted, or represents a pyridyl radical, R3 is hydrogen or lower alkyl and R6 is an aralkyl - As a phenyl-lower alkyl, especially phenylmethyl radical, it being possible for the aryl radicals to be substituted, as just shown.



   The compounds of the formula are also of importance
EMI2.2
 and their tautomeric forms and the salts thereof, wherein R1 is a lower alkyl radical, e.g. B. methyl, ethyl, propyl, isopropyl, butyl- (2), 3-methyl-butyl- (2), pentyl- (2), pentyl- (3), a cycloalkyl radical, e.g. B.



  Cyclopentyl or cyclohexyl, a halo-lower alkyl radical such as chloroethyl, an oxane-lower alkyl radical such as 3-oxapentyl, and R2 is hydrogen or lower alkyl and R6 is an alkyl radical with more than 2 carbon atoms, e.g. B. propyl, isopropyl, butyl, isobutyl, amyl or isoamyl means.



   The compounds of the formula are particularly valuable
EMI2.3
 and their tautomeric forms and salts thereof, in which Rt is a lower alkyl radical, R5 is a lower alkyl radical or, in particular, hydrogen and R6 is an unsubstituted or phenyl radical mono- or tri-substituted benzyl radical by chlorine atoms, methoxy groups, methylenedioxy groups, methyl groups or trifluoromethyl groups.



   Particular mention should be made of 1-isopropyl-4-hydroxy-6-benzyl-pyrazolo [3,4-d] pyrimidine and 1-isopropyl-4-hydroxy-6- (p-chlorobenzyl) pyrazolo [3 4-dipyrimidine , 1-isopropyl-4-hydroxy-6- (m-methoxybenzyl) pyrazolo [3 4-d] pyrimidine, 1-isopropylXhydroxy-6- (3 ', 4', 5'-trimethoxyphenylmethyl) pyrazolo [3,4-djpyrimidine and the 1-pentyl- (3) -4-hydroxy-6-benzyl-pyrazolo- [3,4a] pyrimidine and their salts.



   The inventive method for the preparation of the new compounds consists in that one in a nitrile of the formula
EMI2.4
 the nitrile group converted into the carbamyl group by hydrolysis, e.g. B. by treatment with alkalis in the presence of oxidizing agents such as hydrogen peroxide, and the product obtained is cyclized. The ring closure can take place simultaneously with the conversion into the carbamyl group.



   The 4-hydroxy-pyrazolopyrimidines obtained can be converted into their salts with bases, eg. B. converted into their metal salts such as alkali metal salts, e.g. B. by dissolving in alkaline solutions. The salts, for their part, can be converted into the free hydroxy compounds, conveniently by treatment with acids.



   The new, pharmacologically valuable compounds, their salts or mixtures thereof can, for. B. find use in the form of pharmaceutical preparations. These contain the compounds mentioned in a mixture with a pharmaceutical, organic or inorganic carrier material suitable for enteral or parenteral administration.



   The end products obtained according to the process are also valuable intermediates, eg. B. for the preparation of the 4-mercapto or 4-amiho compounds described in Swiss Patents Nos. 390264 and 390 929.



   If the starting materials used in the process according to the invention are new, they can be produced by methods known per se.



   According to the present invention, the starting materials used are preferably those which lead to the end materials described at the beginning as being particularly valuable.



   In the following example, the temperatures are given in degrees Celsius.



   example
7.95 g of 2-isopropyl-3- (p-chlorophenylacetylamino) 4-pyrazole-carboxylic acid nitrile are heated to 700 for 10 hours with 27.2 cm3 of 10% strength potassium hydroxide solution and 102 cms of 3% hydrogen peroxide.



  The reaction solution is then filtered and acidified to pH 5 with 2N hydrochloric acid, whereupon the 1-isopropyl-4-hydroxy-6- (p-chlorobenzyl) pyrazolo [3,4-d] pyrimidine of the formula
EMI3.1
 separates in colorless crystals from F. 181-182.



   The 2-isopropyl-3- (p-chlorophenylacetylamino) -4-pyrazole-carboxylic acid nitrile used as starting material can be prepared as follows:
To 45.5 g of 2-isopropyl-3-amino-4-cyano-pyrazole in 325 cm3 of abs. Dioxane and 24 cm3 of pyridine are added dropwise with stirring to a solution of 55.8 g of p-chlorophenylacetic acid chloride in 75 cm3 of dioxane at a temperature between 10-150. After the dropwise addition, the mixture is stirred for a further hour at 100 and then for another 2 hours at room temperature.

   After adding 100 cm3 of water and 200 cm3 of 2N hydrochloric acid, the 2-isopropyl-3- (p-chlorophenyl-acetylamino) -4-pyrazolecarboxonitrile of the formula crystallizes
EMI3.2

The following compounds can be obtained in an analogous manner: a) 1-Isopropyl-4-hydroxy-6-benzyl-pyrazolo [3 4-dipyrimidine,
F. 165-166 (from alcohol). b) 1-methyl-4-hydroxy-6-benzyl-pyrazolo [3,4-d] pyrimidine,
F. 236-237 (from ethanol). c) 1-methyl-4-hydroxy-6- (3 ', 4', 5'-trimethoxy phenyl-methyl) -pyrazolo [3,4-d] pyrimidine,
F. 2450 (from chloroform-petroleum ether). d) 1-isopropyl-4-hydroxy-6- (3 ', 4', 5'-trimethoxy phenyl-methyl) -pyrazolo [3,4-d] pyrimidine,
F. 195-196 (from alcohol). e) 1-isopropyl-4-hydroxy-6-p-ethoxybenzyl pyrazolo [3,4-d] pyrimidine,
F. 175-176 (from alcohol). f) 1-sec-butyl-4-hydroxy-6-benzyl-pyrazolo [3,4-d] pyrimidine,
F.

     15P1550 (from alcohol). g) 1-Cyclohexyl-4-hydroxy-6-benzyl-pyrazolo [3, 4-d] pyrimidine,
F. 207-208 (from alcohol). h) 1- (3'-pentyl) -4-hydroxy-6-benzyl-pyrazolo [3 4-dipyrimidine,
F 144-145 (from absolute alcohol). i) 1-Cyclopentyl-4-hydroxy-6-benzyl-pyrazolo [3,4-d] pyrimidine,
F. 189-190 (from abs.

   Alcohol). k) 1- (ss-hydroxy-ethyl) -4-hydroxy-6-benzyl pyrazolo [3,4-d] pyrimidine,
F. 19P1950 (from alcohol). l) 1-isopropyl-4-hydroxy-6- (m-methoxy-benzyl) pyrazolo [3,4-d] pyrimidine,
F. 155-158 (from alcohol). m) 1- [1'-Ethoxy-butyl- (3 ')] - 4-hydroxy-6-benzyl pyrazolo [3,4-d] pyrimidine,
F. 111-112 (from methanol-water). n) l-methyl-4-hydroxy-6-p-chlorobenzyl-pyrazolo- [3 4-dipyrimidine,
F. 268-270 (from dimethylformamide-water). o) 1-methyl-4-hydroxy-6- (2 ', 3'-dimethoxy phenyl-methyl) -pyrazolo [3,4-d] pyrimidine,
F. 190-191 (from alcohol). p) 1-phenyl-4-hydroxy-6-benzyl-pyrazolo [3, 4-d] pyrimidine,
F. 264-265 (from chloroform-petroleum ether). q) 1-phenyl-4-hydroxy-6- (m-methoxybenzyl) pyrazolo [3,4-d] pyrimidine,
F.

   2350 (from chloroform-petroleum ether). r) 1- α-pyridyl-4-hydroxy-6-benzyl-pyrazolo- [3,4-d] pyrimidine,
Q.? 3600 (from dimethylformamide). s) 4-Hydroxy-6-benzyl-pyrazolo [3, 4-d] pyrimidine,
F. 290-292 (from ethanol). t) 1-isopropyl-4-hydroxy-6- (o-methoxy-benzyl) pyrazolo [3,4-d] pyrimidine,
F. 157-159 (from ethanol). u) 1-isopropyl-4-hydroxy-6- (2'-methyl-3'-methoxy-phenyl-methyl) -pyrazolo [3,4-d] pyrimidine,
F. 150-151 (from ethanol). v) l-isopropyl-phydroxy-6-diphenyimethylpyrazolo [3,4-d] pyrimidine,
F. 226-227 (from ethanol). w) 1- [3'-methyl-butyl- (2 ')] - 4-hydroxy-6-benzyl pyrazolo [3,4-d] pyrimidine,
F. 157-158 (from ethanol). x) 1-isopropyl-4-hydroxy-6 - (?

  -phenyl-propyl) - pyrazolo [3,4-d] pyrimidine,
F. 142-143 (from alcohol). y) 1-isopropyl-4-hydroxy-6- (ss-phenyl-ethyl) pyrazolo [3,4-d] pyrimidine,
F. 12P1250 (from alcohol). z) 1-isopropyl-4-hydroxy-6- (m-hydroxy-benzyl) pyrazolo [3,4-d] pyrimidine,
F. 226-227 (from alcohol). aa) 1-isopropyl-4-hydroxy-6-isopropyl-pyrazolo [3,4-dipyrimidine,
F. 175-177 (from ethanol). bb) 1-isopropyl-4-hydroxy-6- (2'-methyl-propyl) pyrazolo [3, 4-d] pyrimidine,
F. 11P1160 (from ethanol). cc) 1-sec-butyl-4-hydroxy-6-isopropyl-pyrazolo- [3 4-dipyrimidine,
F. 146-148 (from ether-petroleum ether). dd) l-sec-butyl-phydroxy-6- (2'-methyl-propy pyrazolo [3,4-d] pyrimidine,
F. 115-116 (from ether-petroleum ether).

 

Claims (1)

PATENTANSPRUCH Verfahren zur Herstellung von Pyrazolo[3,4-d]- pyrimidinen der Formel EMI4.1 worin R6 einen Aralkyl- oder Heterocyclylalkylrest oder einen Alkylrest mit mehr als 2 Kohlenstoffatomen bedeutet, R5 für ein Wasserstoffatom oder einen niederen Alkylrest steht und R1 ein Wasserstoffatom, einen Alkylrest, einen Hydroxyalkylrest, einen Halogenalkylrest, einen Oxaalkyl-, Cycloalkyloder Cycloalkylalkylrest oder einen Aralkyl- oder Heterocyclylalkylrest oder einen höchstens zweikernigen Aryl- oder heterocyclischen Rest bedeutet, oder ihrer Salze, dadurch gekennzeichnet, dass man in einem Nitril der Formel EMI4.2 die Nitrilgruppe durch Hydrolyse in die Carbamylgruppe überführt, und das erhaltene Produkt cyclisiert. PATENT CLAIM Process for the preparation of pyrazolo [3,4-d] pyrimidines of the formula EMI4.1 where R6 is an aralkyl or heterocyclylalkyl radical or an alkyl radical with more than 2 carbon atoms, R5 is a hydrogen atom or a lower alkyl radical and R1 is a hydrogen atom, an alkyl radical, a hydroxyalkyl radical, a haloalkyl radical, an oxaalkyl, cycloalkyl or cycloalkylalkyl radical or an aralkyl radical or Heterocyclylalkylrest or an at most binuclear aryl or heterocyclic radical, or their salts, characterized in that one in a nitrile of the formula EMI4.2 the nitrile group is converted into the carbamyl group by hydrolysis, and the product obtained is cyclized. UNTERANSPRÜCHE 1. Verfahren nach Patentanspruch, dadurch gekennzeichnet, dass man die Hydrolyse durch Behandlung mit Alkalien in Gegenwart von Oxydationsmitteln durchführt. SUBCLAIMS 1. The method according to claim, characterized in that the hydrolysis is carried out by treatment with alkalis in the presence of oxidizing agents. 2. Verfahren nach Unteranspruch 1, dadurch gekennzeichnet, dass man als Oxydationsmittel Wasserstoffsuperoxyd verwendet. 2. The method according to dependent claim 1, characterized in that the oxidizing agent used is hydrogen peroxide. 3. Verfahren nach Patentanspruch oder einem der Unteransprüche 1 und 2, dadurch gekennzeichnet, dass man von Ausgangsstoffen ausgeht, worin R, ein Wasserstoffatom, einen niederen Alkylrest, einen Cycloalkylrest, einen Hydroxyniederalkylrest, einen Halogenniederalkylrest, einen Oxaniederalkylrest oder einen gegebenenfalls durch Halogenatome, niedere Alkoxygruppen, niedere Alkylreste, Methylendioxygruppen oder Trifluoromethylgruppen mono-, die oder trisubstituierten Phenylrest oder einen Pyridylrest, R3 ein Wasserstoffatom oder einen niederen Alkylrest und R6 einen gegebenenfalls im Phenylrest durch Halogenatome, niedere Alkoxygruppen, niedere Alkylreste, Methylendioxygruppen oder Trifluoromethylgruppen mono-, dioder trisubstituierten Phenylniederalkylrest bedeutet. 3. The method according to claim or one of the subclaims 1 and 2, characterized in that starting materials are used in which R is a hydrogen atom, a lower alkyl radical, a cycloalkyl radical, a hydroxy lower alkyl radical, a halo-lower alkyl radical, an oxane-lower alkyl radical or an optionally halogen atom, lower Alkoxy groups, lower alkyl radicals, methylenedioxy groups or trifluoromethyl groups mono-, the or trisubstituted phenyl radical or a pyridyl radical, R3 is a hydrogen atom or a lower alkyl radical and R6 is optionally a phenyl radical through halogen atoms, lower alkoxy groups, lower alkyl radicals, methylenedioxy radicals or trifluoromethyl mono- or trifluoromethyl groups means. 4. Verfahren nach Patentanspruch oder einem der Unteransprüche 1 und 2, dadurch gekennzeichnet, dass man von Ausgangsstoffen ausgeht, worin R1 einen niederen Alkylrest, einen Cycloalkylrest, einen Halogenniederalkylrest oder einen Oxaniederalkylrest bedeutet, R5 für ein Wasserstoffatom oder einen niederen Alkylrest steht und Reinen Alkylrest mit mehr als 2 Kohlenstoffatomen bedeutet. 4. The method according to claim or one of the dependent claims 1 and 2, characterized in that starting materials are used in which R1 is a lower alkyl radical, a cycloalkyl radical, a halo-lower alkyl radical or an oxane-lower alkyl radical, R5 is a hydrogen atom or a lower alkyl radical and is a pure alkyl radical means having more than 2 carbon atoms. 5. Verfahren nach Patentanspruch oder einem der Unteransprüche 1 und 2, dadurch gekennzeichnet, dass man von Ausgangsstoffen ausgeht, worin R1 einen Cycloalkylrest oder einen Alkylrest, R3 ein Wasserstoffatom oder einen niederen Alkylrest und Rr einen niederen Alkylrest mit mindestens 3 Kohlenstoffatomen bedeutet. 5. The method according to claim or one of the dependent claims 1 and 2, characterized in that starting materials are used in which R1 is a cycloalkyl radical or an alkyl radical, R3 is a hydrogen atom or a lower alkyl radical and Rr is a lower alkyl radical with at least 3 carbon atoms. 6. Verfahren nach Patentanspruch oder einem der Unteransprüche 1 und 2, dadurch gekennzeichnet, dass man von Ausgangsstoffen ausgeht, worin R1 einen niederen Alkylrest, R3 ein Wasserstoffatom und R, einen unsubstituierten oder im Phenvlrest durch Chioratome, Methoxygruppen Methyiendioxy- gruppen, Methylgruppen oder Trifluoromethylgruppen mono-, die oder trisubstituierten Benzylrest bedeutet. 6. The method according to claim or one of the dependent claims 1 and 2, characterized in that starting materials are used in which R1 is a lower alkyl radical, R3 is a hydrogen atom and R is an unsubstituted or phenyl radical by chlorine atoms, methoxy groups, methyiendioxy groups, methyl groups or trifluoromethyl groups mono-, the or trisubstituted benzyl radical. 7. Verfahren nach Patentanspruch oder einem der Unteransprüche 1 und 2, dadurch gekennzeichnet, dass man von Verbindungen ausgeht, worin R1 den Isopropylrest, R5 ein Wasserstoffatom und R6 den pChlorbenzylrest bedeutet. 7. The method according to claim or one of the dependent claims 1 and 2, characterized in that one starts from compounds in which R1 is the isopropyl radical, R5 is a hydrogen atom and R6 is the p-chlorobenzyl radical.
CH1311064A 1960-05-11 1960-05-11 Process for the preparation of new pyrazolopyrimidines CH396925A (en)

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