CH276707A - Method and device for growing yeast. - Google Patents
Method and device for growing yeast.Info
- Publication number
- CH276707A CH276707A CH276707DA CH276707A CH 276707 A CH276707 A CH 276707A CH 276707D A CH276707D A CH 276707DA CH 276707 A CH276707 A CH 276707A
- Authority
- CH
- Switzerland
- Prior art keywords
- mash
- dependent
- vat
- yeast
- air
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/22—Perforated plates, discs or walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Mycology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Thermal Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
Verfahren und Einrichtung zur Züchtung von Hefe. Bei den bekannten Ileferiichtungsverfah- ren wird die Hefehaltige Maische durch ein gelochtes Rohrsystem vom Bottichboden ans belüftet. Wenn an Stelle des Rohrsystems feinporige Belüftungskörper benutzt werden, muss der Maische ein mit der aufsteigenden Luft gleichgerichteter Umlauf erteilt werden, da sieh sonst die kleinen Luftbläschen zu grossen Blasen vereinigen.
Der Luftgehalt h' der belüfteten Maische kann nach folgen der Formel berechnet werden:
EMI0001.0006
wobei i"" die Eintrittsgeschwindigkeit der Luft im Botticlrquerschnitt, v" die Aufstiegsge schwindigkeit der Luftbläschen und h das Vo- hirnen der hefehaltigen, unbelüfteten Maisehe bedeutet. Ist v" < ve, so vereinigen sieh die kleinen Bläschen zu grossen Blasen, deren Aiifstiegsgesehwindig-keit dann erheblich grö sser wird als die Eintrittsgeschwindigkeit ve, wodurch der Luftgehalt verringert wird.
Es wurde nun gefunden, dass der Luftge halt. der belüfteten Maische dadurch geregelt und vergrössert werden kann, dass man der Maische eine Abwärtsbewegung erteilt. Ist die Strömungsgeschwindigkeit der Maische v", beispielsweise angenähert gleich der Aufstiegs geschwindigkeit der Bläschen v;" so gilt, wie gefunden wurde, für das Luftvolumen fol gende Formel:
EMI0001.0019
Folgende Tabelle lässt erkennen, dass man durch Einstellung der Umlaufgeschwindigkeit der Maisehe das Luftvolumen in der belüf teten Maisehe ziemlich beliebig regeln kann.
EMI0001.0021
<I>97" <SEP> v, <SEP> 'Um <SEP> <B>h'</B></I>
<tb> .1 <SEP> 1 <SEP> 1 <SEP> 1/1, <SEP> h
<tb> 1 <SEP> \' <SEP> h
<tb> 1 <SEP> \',5 <SEP> ? <SEP> h Von dem Luftinhalt der Maische hängt die Diffusionsoberfläche ab, die für die Versor gung der Hefezellen mit Atmtungssauerstoff massgebend ist. Um die Gegenströmung v", zu erzielen, wird zweckmässig die in dem Bottich unter den Belüftungskörpern befindliche luft freie Maische durch eine Pumpe von oben her wieder in den Bottich zurückgeführt, wobei vorteilhaft die Nährlösung vor der Pumpe der Maisehe zugesetzt wird.
Dadurch wird nicht nur eine intensive Vermischung der Nähr lösung mit der umlaufenden Maische erreicht, sondern darüber hinaus die Verarmung der in den obern Flüssigkeitsschichten aufsteigen den Luftbläschen an Sauerstoff durch die grössere Nährlösungskonzentration kompen siert, während unmittelbar über dem Belüf tungssystem der kleinere Nährlösungsgehalt durch den grösseren Sauerstoffgehalt der Luftbläschen ausgeglichen wird.
Die umlaufende, luftfreie Maische kann ferner zwecks Abführung der während des Verhefungs- und Vergärungsprozesses entste henden Wärmemengen mit einem Kühler ver- sehen sein, wobei die sonst erforderlichen Kühlflächen wesentlich kleiner gehalten wer den können, da die umlaufende Maische prak tisch luftfrei ist.
Zur gleichmässigen Verteilung der mit der Nährlösung vermischten Maische über den ganzen Bottichquerschnitt kann die Maische z. B. durch ein Rohrsystem oder durch Düsen in Flüssigkeitsstrahlen zerteilt werden.
Während des Prozesses entstehen bekannt lich grosse Schaummengen. Um aber die Schaumdicke zu beschränken, ist zweckmässig auf der Flüssigkeit im Bottich ein ringförmi ger Schwimmer mit einer über demselben lie genden durchlöcherten Prellfläche vorgesehen, auf welche die Maischestrahlen auftreffen und den über die Prellfläche steigenden Schaum zerstören.
Es hat sich ferner als vorteilhaft erwiesen, das Belüftungssystem nicht unmittelbar am Boden des Bottichs, sondern in eine Teilhöhe des Verhefungsbottichs anzuordnen, und zwar zweckmässig verstellbar.
Zum Schluss sei erwähnt, dass das erfin dungsgemässe Verfahren sowohl bei Hefezüch- tungsverfahren mit periodischer Entnahme des Zuwachses wie auch mit ganz besonderem Vorteil bei dem Verfahren mit kontinuier licher Entnahme anwendbar ist.
Method and device for growing yeast. In the known Ileferiichtungverfah- ren, the yeast-containing mash is aerated through a perforated pipe system from the bottom of the tub. If fine-pored aeration bodies are used instead of the pipe system, the mash must be circulated in the same direction as the rising air, otherwise the small air bubbles will unite to form large bubbles.
The air content h 'of the aerated mash can be calculated using the following formula:
EMI0001.0006
where i "" means the entry speed of the air in the cross-section of the vat, v "the rate of ascent of the air bubbles and h the braining of the yeast-containing, unventilated corn cows. If v" is ve, the small bubbles combine to form large bubbles, the rise of which is The speed then becomes considerably greater than the entry speed ve, as a result of which the air content is reduced.
It has now been found that the air content. the aerated mash can be regulated and enlarged by giving the mash a downward movement. If the flow rate of the mash v ", for example approximately equal to the ascent rate of the bubbles v;" so, as was found, the following formula applies to the air volume:
EMI0001.0019
The following table shows that you can regulate the volume of air in the ventilated maize hay as you like by adjusting the speed of rotation of the maize coe
EMI0001.0021
<I> 97 "<SEP> v, <SEP> 'Um <SEP> <B>h'</B> </I>
<tb> .1 <SEP> 1 <SEP> 1 <SEP> 1/1, <SEP> h
<tb> 1 <SEP> \ '<SEP> h
<tb> 1 <SEP> \ ', 5 <SEP>? <SEP> h The diffusion surface depends on the air content of the mash, which is decisive for the supply of the yeast cells with respiratory oxygen. In order to achieve the countercurrent v ″, the air-free mash located in the tub under the aeration bodies is expediently returned to the tub from above by a pump, the nutrient solution advantageously being added to the corn herd before the pump.
This not only achieves an intensive mixing of the nutrient solution with the circulating mash, but also compensates for the depletion of oxygen in the air bubbles rising in the upper layers of the liquid due to the greater concentration of nutrient solution, while the smaller nutrient solution content directly above the ventilation system is compensated for by the greater one Oxygen content of the air bubbles is balanced.
The circulating, air-free mash can also be provided with a cooler for the purpose of dissipating the amounts of heat generated during the fermentation and fermentation process, whereby the otherwise required cooling surfaces can be kept much smaller since the circulating mash is practically air-free.
For even distribution of the mash mixed with the nutrient solution over the entire cross-section of the vat, the mash can e.g. B. be divided into jets of liquid by a pipe system or by nozzles.
As is well known, large amounts of foam are produced during the process. But in order to limit the foam thickness, a ringförmi ger float with a perforated baffle surface lying above the same lying lowing on which the mash jets impinge and destroy the foam rising over the baffle is useful on the liquid in the tub.
It has also proven to be advantageous not to arrange the ventilation system directly on the bottom of the vat, but rather in a partial height of the fermentation vat, and to be more specifically adjustable.
Finally, it should be mentioned that the method according to the invention can be used both in yeast cultivation methods with periodic removal of the increment and with particular advantage in the method with continuous removal.
Claims (1)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH276707T | 1949-06-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
CH276707A true CH276707A (en) | 1951-07-31 |
Family
ID=4480884
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CH276707D CH276707A (en) | 1949-06-17 | 1949-06-17 | Method and device for growing yeast. |
Country Status (3)
Country | Link |
---|---|
CH (1) | CH276707A (en) |
DE (1) | DE814134C (en) |
FR (1) | FR1000435A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL79006C (en) * | 1950-03-06 | 1955-09-15 | Eugen Georg Stich |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1015761B (en) * | 1954-09-24 | 1957-09-19 | Dr Vaclav Jonas | Fermentation tank for continuous fermentation in liquids |
DE1045951B (en) * | 1956-04-09 | 1958-12-11 | Ottomar Kurtz G M B H | Device for aerating yeast, wort and alcoholic liquids or the like. |
-
1949
- 1949-06-17 CH CH276707D patent/CH276707A/en unknown
- 1949-11-24 DE DEST277A patent/DE814134C/en not_active Expired
- 1949-11-29 FR FR1000435D patent/FR1000435A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL79006C (en) * | 1950-03-06 | 1955-09-15 | Eugen Georg Stich |
Also Published As
Publication number | Publication date |
---|---|
DE814134C (en) | 1951-09-20 |
FR1000435A (en) | 1952-02-12 |
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