CH276707A - Method and device for growing yeast. - Google Patents

Method and device for growing yeast.

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Publication number
CH276707A
CH276707A CH276707DA CH276707A CH 276707 A CH276707 A CH 276707A CH 276707D A CH276707D A CH 276707DA CH 276707 A CH276707 A CH 276707A
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Switzerland
Prior art keywords
mash
dependent
vat
yeast
air
Prior art date
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German (de)
Inventor
Stich Eugen
Original Assignee
Stich Eugen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Stich Eugen filed Critical Stich Eugen
Publication of CH276707A publication Critical patent/CH276707A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/02Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/12Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18Flow directing inserts
    • C12M27/22Perforated plates, discs or walls
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Thermal Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Description

  

  Verfahren und Einrichtung zur Züchtung von Hefe.    Bei den bekannten     Ileferiichtungsverfah-          ren    wird die     Hefehaltige    Maische durch ein  gelochtes Rohrsystem vom     Bottichboden    ans  belüftet. Wenn an Stelle des Rohrsystems  feinporige     Belüftungskörper    benutzt werden,  muss der Maische ein mit der aufsteigenden  Luft gleichgerichteter Umlauf erteilt werden,  da sieh sonst die kleinen Luftbläschen zu  grossen Blasen vereinigen.

   Der Luftgehalt  h' der belüfteten Maische kann nach folgen  der Formel berechnet werden:  
EMI0001.0006     
    wobei     i""    die Eintrittsgeschwindigkeit der Luft  im     Botticlrquerschnitt,        v"    die Aufstiegsge  schwindigkeit der Luftbläschen und h das     Vo-          hirnen    der hefehaltigen,     unbelüfteten    Maisehe  bedeutet. Ist     v"     <      ve,    so vereinigen sieh die  kleinen Bläschen zu grossen Blasen, deren       Aiifstiegsgesehwindig-keit    dann erheblich grö  sser wird als die Eintrittsgeschwindigkeit     ve,     wodurch der Luftgehalt verringert wird.  



  Es wurde nun gefunden, dass der Luftge  halt. der belüfteten Maische dadurch geregelt  und vergrössert werden kann, dass man der  Maische eine Abwärtsbewegung erteilt. Ist  die Strömungsgeschwindigkeit der Maische     v",     beispielsweise angenähert gleich der Aufstiegs  geschwindigkeit der Bläschen     v;"    so gilt, wie  gefunden wurde, für das Luftvolumen fol  gende Formel:  
EMI0001.0019     
    Folgende Tabelle lässt erkennen, dass man  durch Einstellung der Umlaufgeschwindigkeit       der    Maisehe das Luftvolumen in der belüf  teten Maisehe ziemlich beliebig regeln kann.

    
EMI0001.0021     
  
    <I>97" <SEP> v, <SEP> 'Um <SEP> <B>h'</B></I>
<tb>  .1 <SEP> 1 <SEP> 1 <SEP> 1/1, <SEP> h
<tb>  1 <SEP> \' <SEP> h
<tb>  1 <SEP> \',5 <SEP> ? <SEP> h       Von dem     Luftinhalt    der Maische hängt die  Diffusionsoberfläche ab, die für die Versor  gung der Hefezellen mit     Atmtungssauerstoff     massgebend ist. Um die Gegenströmung     v",    zu  erzielen, wird zweckmässig die in dem Bottich  unter den Belüftungskörpern befindliche luft  freie Maische durch eine Pumpe von oben her  wieder in den Bottich zurückgeführt, wobei  vorteilhaft die Nährlösung vor der Pumpe der  Maisehe zugesetzt wird.

   Dadurch wird nicht  nur eine intensive Vermischung der Nähr  lösung mit der umlaufenden Maische erreicht,  sondern darüber hinaus die Verarmung der  in den obern Flüssigkeitsschichten aufsteigen  den Luftbläschen an Sauerstoff durch die  grössere     Nährlösungskonzentration    kompen  siert, während unmittelbar über dem Belüf  tungssystem der kleinere     Nährlösungsgehalt     durch den grösseren Sauerstoffgehalt der  Luftbläschen ausgeglichen wird.  



  Die umlaufende, luftfreie     Maische    kann  ferner zwecks Abführung der während     des          Verhefungs-    und     Vergärungsprozesses    entste  henden Wärmemengen mit einem Kühler ver-      sehen sein, wobei die sonst erforderlichen  Kühlflächen wesentlich kleiner gehalten wer  den können, da die umlaufende Maische prak  tisch luftfrei ist.  



  Zur gleichmässigen Verteilung der mit der  Nährlösung vermischten Maische über den  ganzen     Bottichquerschnitt    kann die Maische  z. B. durch ein Rohrsystem oder durch Düsen  in Flüssigkeitsstrahlen zerteilt werden.  



  Während des Prozesses entstehen bekannt  lich grosse Schaummengen. Um aber die  Schaumdicke zu beschränken, ist zweckmässig  auf der Flüssigkeit im Bottich ein ringförmi  ger Schwimmer mit einer über demselben lie  genden durchlöcherten Prellfläche vorgesehen,  auf welche die     Maischestrahlen    auftreffen und  den über die Prellfläche steigenden     Schaum     zerstören.  



  Es hat sich ferner als vorteilhaft erwiesen,  das Belüftungssystem nicht unmittelbar am  Boden des Bottichs, sondern in eine Teilhöhe  des     Verhefungsbottichs    anzuordnen, und zwar  zweckmässig verstellbar.  



  Zum Schluss sei erwähnt, dass das erfin  dungsgemässe Verfahren sowohl bei     Hefezüch-          tungsverfahren    mit periodischer Entnahme  des Zuwachses wie auch mit ganz besonderem  Vorteil bei dem     Verfahren    mit kontinuier  licher Entnahme anwendbar ist.



  Method and device for growing yeast. In the known Ileferiichtungverfah- ren, the yeast-containing mash is aerated through a perforated pipe system from the bottom of the tub. If fine-pored aeration bodies are used instead of the pipe system, the mash must be circulated in the same direction as the rising air, otherwise the small air bubbles will unite to form large bubbles.

   The air content h 'of the aerated mash can be calculated using the following formula:
EMI0001.0006
    where i "" means the entry speed of the air in the cross-section of the vat, v "the rate of ascent of the air bubbles and h the braining of the yeast-containing, unventilated corn cows. If v" is ve, the small bubbles combine to form large bubbles, the rise of which is The speed then becomes considerably greater than the entry speed ve, as a result of which the air content is reduced.



  It has now been found that the air content. the aerated mash can be regulated and enlarged by giving the mash a downward movement. If the flow rate of the mash v ", for example approximately equal to the ascent rate of the bubbles v;" so, as was found, the following formula applies to the air volume:
EMI0001.0019
    The following table shows that you can regulate the volume of air in the ventilated maize hay as you like by adjusting the speed of rotation of the maize coe

    
EMI0001.0021
  
    <I> 97 "<SEP> v, <SEP> 'Um <SEP> <B>h'</B> </I>
<tb> .1 <SEP> 1 <SEP> 1 <SEP> 1/1, <SEP> h
<tb> 1 <SEP> \ '<SEP> h
<tb> 1 <SEP> \ ', 5 <SEP>? <SEP> h The diffusion surface depends on the air content of the mash, which is decisive for the supply of the yeast cells with respiratory oxygen. In order to achieve the countercurrent v ″, the air-free mash located in the tub under the aeration bodies is expediently returned to the tub from above by a pump, the nutrient solution advantageously being added to the corn herd before the pump.

   This not only achieves an intensive mixing of the nutrient solution with the circulating mash, but also compensates for the depletion of oxygen in the air bubbles rising in the upper layers of the liquid due to the greater concentration of nutrient solution, while the smaller nutrient solution content directly above the ventilation system is compensated for by the greater one Oxygen content of the air bubbles is balanced.



  The circulating, air-free mash can also be provided with a cooler for the purpose of dissipating the amounts of heat generated during the fermentation and fermentation process, whereby the otherwise required cooling surfaces can be kept much smaller since the circulating mash is practically air-free.



  For even distribution of the mash mixed with the nutrient solution over the entire cross-section of the vat, the mash can e.g. B. be divided into jets of liquid by a pipe system or by nozzles.



  As is well known, large amounts of foam are produced during the process. But in order to limit the foam thickness, a ringförmi ger float with a perforated baffle surface lying above the same lying lowing on which the mash jets impinge and destroy the foam rising over the baffle is useful on the liquid in the tub.



  It has also proven to be advantageous not to arrange the ventilation system directly on the bottom of the vat, but rather in a partial height of the fermentation vat, and to be more specifically adjustable.



  Finally, it should be mentioned that the method according to the invention can be used both in yeast cultivation methods with periodic removal of the increment and with particular advantage in the method with continuous removal.

 

Claims (1)

PATENTANSPRÜCHE: I. Verfahren zur Züchtung von Hefe in Bottichen unter Belüftung durch aufsteigende Luftbläschen, dadurch gekennzeichnet, dass die hefehaltige Maische abwärts durch den Bottich bewegt, unten dem Bottich entnom inen und von oben her wieder zugeführt wird. I1. Einrichtung zur Durchführung des Verfahrens nach dem Patentanspruch I, da durch gekennzeichnet, dass zwischen der am Boden des Bottichs befindlichen Ablaufstelle für die Maisehe und der an dessen obern Ende befindlicher Eintrittsstelle eine Umlauf pumpe eingeschaltet ist. PATENT CLAIMS: I. A method for growing yeast in vats with aeration by ascending air bubbles, characterized in that the yeast-containing mash is moved downward through the vat, removed from the vat at the bottom and fed back in from above. I1. Device for carrying out the method according to claim I, characterized in that a circulation pump is switched on between the outlet point for the corn cows located at the bottom of the tub and the entry point located at its upper end. UNTERANSPRÜCHE: 1. Verfahren nach Patentanspruch I, da durch gekennzeichnet, dass die Nährlösung der rücklaufenden, hefehaltigen Maische zugesetzt und innig mit ihr vermischt wird. 9. Verfahren nach Patentanspruch I und Unteranspruch 1, dadurch gekennzeichnet, dass die dem Bottich von oben zuströmende Maische gleichmässig über die Oberfläche der Maische verteilt wird. 3. Verfahren nach Patentanspruch I und den Unteransprüchen 1 und 2, dadurch ge kennzeichnet, dass die ausserhalb des Bottichs zurückströmende 1Iaische gekühlt. wird. 1. SUBClaims: 1. The method according to claim I, characterized in that the nutrient solution is added to the returning yeast-containing mash and is intimately mixed with it. 9. The method according to claim I and dependent claim 1, characterized in that the mash flowing into the vat from above is distributed evenly over the surface of the mash. 3. The method according to claim I and the dependent claims 1 and 2, characterized in that the outside of the tub flowing back 1Iaische cooled. becomes. 1. Verfahren nach Patentanspruch I und den Unteransprüchen 1 bis 3, dadurch ge kennzeichnet, da.ss die Einführung der Luft in die Maische in einem Abstand vom Boden des Bottichs erfolgt. 5. Einrichtung nach Patentanspruch<B>11,</B> dadurch gekennzeichnet, dass am obern Ende des Bottichs eine Verteilereinrichtung ange ordnet ist, so dass die einströmende Maische in Flüssigkeitsstrahlen gleichmässig über den Bottichquersehnitt verteilt werden kann. 6. Einrichtung nach Patentanspruch Il, dadurch gekennzeichnet, dass in der R.ücklauf- leitung für die Maische eine Kühleinrichtung vorgesehen ist. 7. Method according to claim 1 and the dependent claims 1 to 3, characterized in that the air is introduced into the mash at a distance from the bottom of the vat. 5. Device according to claim 11, characterized in that a distributor device is arranged at the upper end of the tub so that the inflowing mash can be evenly distributed over the tub cross-section in jets of liquid. 6. Device according to claim II, characterized in that a cooling device is provided in the return line for the mash. 7th Einrichtung nach Patentanspruch 1I, dadurch gekennzeichnet, dass in der Rüeklauf- leitung für die Maische vor der Umlaufpumpe Mittel zur Zuführung von Nährlösung vorge sehen sind. B. Device according to Patent Claim 1I, characterized in that means for supplying nutrient solution are provided in the return line for the mash upstream of the circulation pump. B. Einrichtung nach Patentanspruch 11 und Unteranspruch 5, dadurch gekennzeich net, da.ss zur Aufrechterhaltung einer be stimmten Schaumdeckendicke auf der Flüssig keit ein eine durehlöeherte Prellfläche tragen der ringförmiger Schwimmer angeordnet ist, so da.ss die von oben auftreffenden Flüssig keitsstrahlen den über die Prellfläche aufstei genden Schaum zerstören. 9. Einrichtung nach Patentanspruch 1I und den Unteransprüchen 5 bis 8, dadurch ge kennzeichnet, dass die Mittel zur Einführung der Luft in die Maische in einem Abstand über dem Boden des Bottichs angeordnet sind. 10. Device according to patent claim 11 and dependent claim 5, characterized in that, in order to maintain a certain foam cover thickness on the liquid, an annular float is arranged to wear a perforated baffle surface so that the jets of liquid falling from above over the baffle surface destroy rising foam. 9. Device according to claim 1I and the dependent claims 5 to 8, characterized in that the means for introducing the air into the mash are arranged at a distance above the bottom of the vat. 10. Einrichtung nach Patentanspruch II und den Unteransprüchen 5 bis 9, dadurch gekennzeichnet, dass die Mittel zur Einfüh rung der Luft in die Maisehe in der Höhe verstellbar angeordnet sind. 11. Einrichtung nach Patentanspruch Il und den Unteransprüchen 5 bis 10, dadurch gekennzeichnet, dass sie Mittel zur kontinuier- liehen Entnahme von Hefe aufweist. Device according to patent claim II and the dependent claims 5 to 9, characterized in that the means for introducing the air into the maize coarse are arranged adjustable in height. 11. Device according to claim II and the subclaims 5 to 10, characterized in that it has means for continuously borrowed removal of yeast.
CH276707D 1949-06-17 1949-06-17 Method and device for growing yeast. CH276707A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH276707T 1949-06-17

Publications (1)

Publication Number Publication Date
CH276707A true CH276707A (en) 1951-07-31

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Application Number Title Priority Date Filing Date
CH276707D CH276707A (en) 1949-06-17 1949-06-17 Method and device for growing yeast.

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CH (1) CH276707A (en)
DE (1) DE814134C (en)
FR (1) FR1000435A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL79006C (en) * 1950-03-06 1955-09-15 Eugen Georg Stich

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1015761B (en) * 1954-09-24 1957-09-19 Dr Vaclav Jonas Fermentation tank for continuous fermentation in liquids
DE1045951B (en) * 1956-04-09 1958-12-11 Ottomar Kurtz G M B H Device for aerating yeast, wort and alcoholic liquids or the like.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL79006C (en) * 1950-03-06 1955-09-15 Eugen Georg Stich

Also Published As

Publication number Publication date
DE814134C (en) 1951-09-20
FR1000435A (en) 1952-02-12

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