CH214112A - Process for the production of bacterial strains. - Google Patents

Process for the production of bacterial strains.

Info

Publication number
CH214112A
CH214112A CH214112DA CH214112A CH 214112 A CH214112 A CH 214112A CH 214112D A CH214112D A CH 214112DA CH 214112 A CH214112 A CH 214112A
Authority
CH
Switzerland
Prior art keywords
bacteria
nutrient medium
production
bacterial strains
medium
Prior art date
Application number
Other languages
German (de)
Inventor
Gesellschaft Fuer Chemis Basel
Original Assignee
Chem Ind Basel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chem Ind Basel filed Critical Chem Ind Basel
Publication of CH214112A publication Critical patent/CH214112A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/09—Lactobacillales, e.g. aerococcus, enterococcus, lactobacillus, lactococcus, streptococcus
    • A61K39/092—Streptococcus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Description

  

  



  Verfahren zur Herstellung von Bakterienstämmen.



   Die bekannten Nährboden bestehen aus   Alischungen    von zum Beispiel Agar-Agar, Gelatine, gekochtem Serum, durch Hitze be  handelten    Organen bezw.   Organextrakten    oder dergleichen. Solche   Nährböden    enthalten demnach von vornherein   körperfremde    Stoffe oder dann aus körpereigenen Stoffen in   kör-    perfremde übergeführte Stoffe.



   Es wurde nun gefunden, daB man zu neuartigen Bakterienstämmen gelangen kann, wenn man Bakterien auf Plasma in nicht körperfremden Medium züchtet und die er  haltenen    Bakterien von der abscheidbaren   Nährbodenflüssigkeit    trennt.



   Bakterien mit besonderer Wirkung werden derart gewonnen, dass man dem genannten Nährboden steriles Organ oder auch sterile Extrakte von Organen zusetzt.



  Da manche Bakterien bei Zusatz bestimmter Organe kein gutes Wachstum zeigen, ist es für bestimmte Zwecke angezeigt, den Zusatz des sterilen Organes oder des Organextraktes erst in einem Zeitpunkte vorzunehmen, wenn sich die Bakterien in dem Nährboden ohne diesen Zusatz gut entwickelt haben, das heisst nach Bebrütung.



   Das für die Nährboden erforderliche Plasma kann zum Beispiel durch sterile Ent  nahme    des Blutes von Tieren gewonnen oder auch nachträglich durch Filtration oder andere   MaBnahme    sterilisiert werden. Das Plasma kann sowohl in flüssiger wie auch fester Form Verwendung finden. In letzterer Form gewinnt man es leicht zum Beispiel durch Zusatz körpereigener,   gerinnungsför-    dernder Stoffe wie Thrombin oder solches enthaltender tierischer   Nativpräparate.   



   Die nach diesem Verfahren unter wechselnder Zusammensetzung des Nährbodens gewonnenen Bakterien rufen am Tier ver  schiedenartig    verlaufende Krankheiten hervor. Man kann also auf diese Weise Bakterien gewinnen, die spezifisch differenzierte krankmachende Eigenschaften besitzen. Dies hat besondere Bedeutung für die Herstellung von spezifischen Vaccinen und Immunseren. I) ie so   gezüchteten    Bakterien können auf dem gleichen Nährboden weiter iiberimpft werden.



   Beispiel :
Streptococcen, deren Virulenz an der Maus und am Kaninchen geprüft war, werden mit mehrfachen   Uberimpfungen    je 6 bis 8 Tage lang auf   Plasmanährboden    gezüchtet.



  Nach dieser Zeit wird wiederum die Virulenz geprüft. Es zeigt sich dabei, da? diese in bezug auf MÏuse voll erhalten blieb, dass sie aber in bezug auf Kaninchen eine Zunahme erfahren hat, indem sie akut tödliche   Erkran-      kungen    in gro?en Verdünnungen hervorruft, während von der Ziichtung nur chronische Erkrankungen erzielt wurden.   I)    ie erhaltenen Bakterien werden von der NÏhrbodenfl issigkeit abgetrennt.



  



  Process for the production of bacterial strains.



   The well-known nutrient medium consist of Alischungen of, for example, agar-agar, gelatin, boiled serum, BEZW organs treated by heat. Organ extracts or the like. Such nutrient media therefore contain exogenous substances from the outset or then from endogenous substances into exogenous substances.



   It has now been found that novel bacterial strains can be obtained if bacteria are grown on plasma in a non-exogenous medium and the bacteria obtained are separated from the separable culture medium.



   Bacteria with special effects are obtained in such a way that sterile organ or sterile extracts of organs are added to the above-mentioned nutrient medium.



  Since some bacteria do not grow well when certain organs are added, it is advisable for certain purposes to add the sterile organ or the organ extract only at a point in time when the bacteria in the nutrient medium have developed well without this addition, i.e. after Incubation.



   The plasma required for the nutrient medium can, for example, be obtained by sterile extraction of blood from animals or it can also be subsequently sterilized by filtration or other measures. The plasma can be used in both liquid and solid form. In the latter form, it is easy to obtain, for example, by adding endogenous, coagulation-promoting substances such as thrombin or native animal preparations containing such.



   The bacteria obtained by this process with changing composition of the nutrient medium cause various diseases in animals. In this way, bacteria can be obtained that have specifically differentiated disease-causing properties. This is of particular importance for the production of specific vaccines and immune sera. The bacteria thus grown can be further inoculated on the same culture medium.



   Example:
Streptococci, the virulence of which has been tested in mice and rabbits, are cultured on plasma culture medium for 6 to 8 days each with multiple inoculations.



  After this time, the virulence is checked again. It shows that there? this was fully preserved in relation to mice, but it has increased in relation to rabbits, in that it causes acute fatal diseases in large dilutions, whereas breeding only causes chronic diseases. I) The bacteria obtained are separated from the nutrient liquid.

Claims (1)

PATENTANSPRUCH : : Verfahren zur Herstellung von neuarti- gen Bakterienstämmen, dadurch gekennzeich- net, da¯ man Bakterien auf Plasma in nicht körperfremdem Medium züchtet und die erhaltenen Bakterien von der abscheidbaren Nährbodenflüssigkeit abtrennt. PATENT CLAIM:: Process for the production of novel bacterial strains, characterized in that bacteria are grown on plasma in a non-exogenous medium and the bacteria obtained are separated from the separable culture medium. UNTERANSPRUCHE : 1. Verfahren nach Patentanspruch, dadurch gekennzeichnet, da¯ man dem Nährboden steriles Organ zusetzt. SUBClaims: 1. The method according to claim, characterized in that sterile organ is added to the nutrient medium. 2. Verfahren nach Patentanspruch, dadurch gekennzeichnet, dass man dem Nährboden steriles Extrakt von Organen zusetzt. 2. The method according to claim, characterized in that sterile extract from organs is added to the nutrient medium. 3. Verfahren nach Patentanspruch und Unteranspruch l, dadurch gekennzeichnet, da¯ man den Zusatz zum Nährboden nach Bebrütung vornimmt. 3. The method according to claim and dependent claim l, characterized in that the addition to the nutrient medium is made after incubation. 4. Verfahren nach Patentanspruch und ITnteranspruch 2, dadurch gekennzeichnet, daB man den Zusatz zum Nährboden nach Bebrütung vornimmt. 4. The method as claimed in patent claim and sub-claim 2, characterized in that the addition to the nutrient medium is made after incubation.
CH214112D 1937-05-19 1937-05-19 Process for the production of bacterial strains. CH214112A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH214112T 1937-05-19
CH210423T 1937-05-19

Publications (1)

Publication Number Publication Date
CH214112A true CH214112A (en) 1941-03-31

Family

ID=25724855

Family Applications (1)

Application Number Title Priority Date Filing Date
CH214112D CH214112A (en) 1937-05-19 1937-05-19 Process for the production of bacterial strains.

Country Status (1)

Country Link
CH (1) CH214112A (en)

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