CA3219470A1 - Nanopore proteomics - Google Patents

Nanopore proteomics Download PDF

Info

Publication number
CA3219470A1
CA3219470A1 CA3219470A CA3219470A CA3219470A1 CA 3219470 A1 CA3219470 A1 CA 3219470A1 CA 3219470 A CA3219470 A CA 3219470A CA 3219470 A CA3219470 A CA 3219470A CA 3219470 A1 CA3219470 A1 CA 3219470A1
Authority
CA
Canada
Prior art keywords
pore
nanopore
prot
swiss
position corresponding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3219470A
Other languages
English (en)
French (fr)
Inventor
Florian Leonardus Rudolfus LUCAS
Roderick Corstiaan Abraham VERSLOOT
Giovanni Maglia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rijksuniversiteit Groningen
Original Assignee
Rijksuniversiteit Groningen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rijksuniversiteit Groningen filed Critical Rijksuniversiteit Groningen
Publication of CA3219470A1 publication Critical patent/CA3219470A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CA3219470A 2021-05-18 2022-05-18 Nanopore proteomics Pending CA3219470A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP21174437 2021-05-18
EP21174437.0 2021-05-18
PCT/NL2022/050266 WO2022245209A2 (en) 2021-05-18 2022-05-18 Nanopore proteomics

Publications (1)

Publication Number Publication Date
CA3219470A1 true CA3219470A1 (en) 2022-11-24

Family

ID=76011701

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3219470A Pending CA3219470A1 (en) 2021-05-18 2022-05-18 Nanopore proteomics

Country Status (10)

Country Link
US (1) US20240159768A1 (https=)
EP (1) EP4341278A2 (https=)
JP (1) JP2024522073A (https=)
KR (1) KR20240010007A (https=)
CN (1) CN117957243A (https=)
AU (1) AU2022277010A1 (https=)
CA (1) CA3219470A1 (https=)
IL (1) IL308635A (https=)
MX (1) MX2023013655A (https=)
WO (1) WO2022245209A2 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202112235D0 (en) * 2021-08-26 2021-10-13 Oxford Nanopore Tech Ltd Nanopore
EP4412624A4 (en) 2021-10-04 2025-08-20 Neuvivo Inc TREATMENT METHODS FOR ALS PATIENTS
KR20250116014A (ko) 2022-10-28 2025-07-31 리엑스유니버시테이트 그로닝겐 나노기공-기반 단백질 분석
CN120476132A (zh) * 2022-12-29 2025-08-12 深圳华大生命科学研究院 新型孔蛋白bcp34、其突变体及其应用
GB202305524D0 (en) * 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
CN120917151A (zh) * 2023-04-28 2025-11-07 清华大学 一种被修饰的多肽、多肽复合物、多肽混合物及控制多肽移位速度或表征多肽的系统和方法
WO2025003492A1 (en) * 2023-06-30 2025-01-02 Ecole Polytechnique Federale De Lausanne (Epfl) Uses of aerolysin nanopores
EP4491190A1 (en) * 2023-07-14 2025-01-15 Fundación Imdea Alimentación Small proteins for use as senolytic and senomorphic agents
WO2025138302A1 (zh) * 2023-12-30 2025-07-03 深圳华大生命科学研究院 蛋白测序文库的构建方法及蛋白质的测序方法
WO2025229321A1 (en) * 2024-04-30 2025-11-06 Portal Biotech Ltd. Nanopores and uses thereof for analysis of analytes
WO2026052817A1 (en) * 2024-09-06 2026-03-12 Oxford Nanopore Technologies Plc Method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0820927D0 (en) * 2008-11-14 2008-12-24 Isis Innovation Method
WO2020055246A1 (en) * 2018-09-11 2020-03-19 Rijksuniversiteit Groningen Biological nanopores having tunable pore diameters and uses thereof as analytical tools
CN112500459B (zh) * 2020-02-29 2023-04-07 南京大学 Aerolysin突变纳米孔及其在分子生物检测方面的应用
CN112480204A (zh) * 2020-04-13 2021-03-12 南京大学 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法

Also Published As

Publication number Publication date
WO2022245209A2 (en) 2022-11-24
IL308635A (en) 2024-01-01
KR20240010007A (ko) 2024-01-23
US20240159768A1 (en) 2024-05-16
EP4341278A2 (en) 2024-03-27
WO2022245209A3 (en) 2022-12-29
AU2022277010A1 (en) 2023-12-14
MX2023013655A (es) 2024-02-06
JP2024522073A (ja) 2024-06-11
CN117957243A (zh) 2024-04-30

Similar Documents

Publication Publication Date Title
US20240159768A1 (en) Nanopore proteomics
AU2019337340B2 (en) Biological nanopores having tunable pore diameters and uses thereof as analytical tools
Lucas et al. The manipulation of the internal hydrophobicity of FraC nanopores augments peptide capture and recognition
CN107109479B (zh) 具有改变的特征的α-溶血素变体
US20240288416A1 (en) Artificial nanopores and uses and methods relating thereto
EP3049814B1 (en) Method for determining the number or position(s) of two or more post-translational modifications (ptms) in a peptide, polypeptide or protein
AU2015310913A1 (en) Mutant CsgG pores
WO2025046236A1 (en) Methods for nanopore-based analyte detection
Wang et al. Reconstructed protein arrays from 3D HPLC/tandem mass spectrometry and 2D gels: complementary approaches to Porphyromonas gingivalis protein expression
US20260002925A1 (en) Nanopore systems and methods for single-molecule polymer profiling
Bonini et al. Single-molecule identification of full-length proteins with single-amino-acid resolution using nanopores
WO2025230413A1 (en) Ion-selective nanopore systems and methods for single-molecule polymer profiling.
Marekov Determination of Protein Contacts by Chemical Cross‐Linking With EDC and Mass Spectrometry
CN121758562A (zh) 一种极性带电残基工程化的MspA纳米孔及其制备方法和应用
Cordwell Advances in bacterial proteome analysis
Cordwell Microbial proteomics: how far have we come?

Legal Events

Date Code Title Description
MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 3RD ANNIV.) - STANDARD

Year of fee payment: 3

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250512

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250512