CA3213718A1 - Linked amplification tethered with exponential radiance - Google Patents

Linked amplification tethered with exponential radiance Download PDF

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Publication number
CA3213718A1
CA3213718A1 CA3213718A CA3213718A CA3213718A1 CA 3213718 A1 CA3213718 A1 CA 3213718A1 CA 3213718 A CA3213718 A CA 3213718A CA 3213718 A CA3213718 A CA 3213718A CA 3213718 A1 CA3213718 A1 CA 3213718A1
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CA
Canada
Prior art keywords
probe
probes
tertiary
readout
primary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3213718A
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English (en)
French (fr)
Inventor
Long Cai
Chee Huat Eng
Lincoln OMBELETS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology
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California Institute of Technology
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Filing date
Publication date
Application filed by California Institute of Technology filed Critical California Institute of Technology
Publication of CA3213718A1 publication Critical patent/CA3213718A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2543/00—Reactions characterised by the reaction site, e.g. cell or chromosome
    • C12Q2543/10—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA3213718A 2021-05-24 2022-03-24 Linked amplification tethered with exponential radiance Pending CA3213718A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163192554P 2021-05-24 2021-05-24
US63/192,554 2021-05-24
PCT/US2022/021826 WO2022250774A1 (en) 2021-05-24 2022-03-24 Linked amplification tethered with exponential radiance

Publications (1)

Publication Number Publication Date
CA3213718A1 true CA3213718A1 (en) 2022-12-01

Family

ID=81308076

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3213718A Pending CA3213718A1 (en) 2021-05-24 2022-03-24 Linked amplification tethered with exponential radiance

Country Status (7)

Country Link
US (1) US20240240237A1 (https=)
EP (1) EP4347874A1 (https=)
JP (1) JP2024520987A (https=)
KR (1) KR20240013728A (https=)
CN (1) CN117460837A (https=)
CA (1) CA3213718A1 (https=)
WO (1) WO2022250774A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025007154A1 (en) * 2023-06-30 2025-01-02 California Institute Of Technology Signal amplification methods and compositions for molecular target detection by iterative probe deposition
WO2025194315A1 (zh) * 2024-03-18 2025-09-25 深圳华大智造科技股份有限公司 核酸检测方法及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US8481268B2 (en) * 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
WO2011038403A1 (en) * 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
US8658361B2 (en) * 2010-10-21 2014-02-25 Advanced Cell Diagnostics, Inc. Ultra sensitive method for in situ detection of nucleic acids
CN103649331A (zh) * 2011-02-15 2014-03-19 莱卡生物系统纽卡斯尔有限责任公司 用于mrna的定位的原位检测的方法
CN116200462B (zh) * 2016-05-16 2025-10-03 布鲁克空间生物学公司 用于检测样品中目标核酸的方法
EP4556575A3 (en) * 2016-11-21 2025-10-08 Bruker Spatial Biology, Inc. A method for sequencing nucleic acids
CN118345145A (zh) * 2016-12-09 2024-07-16 乌尔蒂维尤股份有限公司 用于使用标记的核酸成像剂进行多路复用成像的改进方法
EP4063519B1 (en) * 2018-04-09 2023-09-13 Advanced Cell Diagnostics, Inc. Methods to further enhance signal amplification for the in situ detection of nucleic acids
EP3894583A4 (en) * 2018-12-13 2022-11-09 President and Fellows of Harvard College AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS

Also Published As

Publication number Publication date
JP2024520987A (ja) 2024-05-28
EP4347874A1 (en) 2024-04-10
WO2022250774A9 (en) 2023-04-13
KR20240013728A (ko) 2024-01-30
US20240240237A1 (en) 2024-07-18
WO2022250774A1 (en) 2022-12-01
CN117460837A (zh) 2024-01-26

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