CA3209006A1 - Methods for the treatment of gpp - Google Patents

Abstract

The present invention relates to the treatment of or alleviation of signs and symptoms of GPP or an acute phase flare-up of generalized pustular psoriasis (GPP) with anti-IL36R antibodies.

Description

METHODS FOR THE TREATMENT OF GPP
SEQUENCE LISTING
[0001] The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on January 31, 2022, is named 09-0719-W0-1-2022-03-03-SL.txt and is 147,456 bytes in size.
TECHNICAL FIELD OF THE INVENTION

[0002] The present invention relates to use of anti-IL-36R antibodies in methods and compositions for treatment of patients with generalized pustular psoriasis (GPP).
More specifically, the invention relates to the treatment of GPP or GPP flares in a patient by administering to the patient two 900 mg intravenous (i.v.) doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose. More specifically, the invention relates to the treatment of GPP
in a patient by administering to the patient a single 900 mg intravenous dose of spesolimab, if the GPP symptoms persist, administering an additional 900 mg intravenous dose one week after the initial dose.
BACKGROUND

[0003] GPP is a severe skin disease characterized by the repeated occurrence of acute flares caused by systemic inflammation affecting the skin and internal organs.
The classic presentation of acute GPP was first described as a recurrent pustular form of psoriasis by von Zumbusch in 1909. While GPP and plaque psoriasis can occur at the same time in an individual patient, GPP is distinct from plaque psoriasis in clinical presentation, pathophysiology, histopathology, response to therapies, epidemiology and genetics.

[0004] Therefore, it is very critical to differentiate GPP from plaque or erythrodermic psoriasis with secondary pustulation. The clinical presentation of GPP
is quite different from psoriasis vulgaris (PV) in its' episodic nature, often with normal appearing skin between very acute and severe disease flares. GPP is clinically characterized by the preponderance of pustules as the primary lesion on an erythematous base rather than red plaques covered with silvery scales representing the primary lesion of typical plaque psoriasis. In addition, the histopathological hallmarks of GPP are distinct spongiform pustules of Kogoj located in the subcorneal portion of the epidermis. GPP may be associated with systemic symptoms (fever, increased CRP and neutrophilia) and severe extra-cutaneous organ manifestations (liver, kidney failure, CV shock). While patients with GPP may have pre-existing or co-existing PV, it is possible to clinically distinguish patients with primary plaque disease (PV) who have a secondary pustular component from patients who have primary pustular disease (GPP) with a concomitant plaque component, based on the sequence of manifestations (primary lesion pustule rather than plaque) and the localization of a GPP pustule on an erythematous base rather than a Ps0 plaque.

[0005] As descriptions for GPP are discordant among standard dermatology textbooks, the European Rare And Severe Psoriasis Expert Network (ERASPEN) has defined consensus criteria that include as key diagnosis criteria for acute GPP the presence of primary, sterile, macroscopically visible pustules on non-acral skin (excluding cases where pustulation is restricted to psoriatic plaques), with or without systemic inflammation, with or without plaque-type psoriasis, either relapsing (>1 episode) or persistent (>3 months).

[0006] Chronic GPP describes the state in between disease flares that may be characterized by the complete absence of symptoms or the persistence of residual skin symptoms such as erythema and scaling and minor pustulation.

[0007] Current treatment options for controlling acute GPP and subcutaneous of response are limited and do not provide sustained efficacy. No treatments are currently approved for GPP in the US and EU, though retinoids, cyclosporine or methotrexate are being recommended. Although these treatments are described to be "remarkably effective or effective" in 70 ¨ 84% of patients (J Am Acad Dermatol.
2012;67(2):279-88) these data are based on a retrospective cohort study from Japan without clearly defined endpoints (Japanese Journal of Dermatology.
2010;120(4):815-39). Furthermore, these treatments cannot be used long-term due to side effects and contraindications (retinoids: teratogenicity, hair loss;
cyclosporine:
excessive hair growth, renal toxicity; MTX: liver toxicity).

[0008] Biologics (mostly TNF inhibitors, occasionally IL-1 or IL-17 inhibitors) are increasingly used to treat more severe, extensive or treatment resistant patients with GPP, based on small published case series. However, these drugs are also associated with limitations in efficacy (incomplete and delayed responses are frequent) and safety as well as contraindications (infusion reactions, tuberculosis, cardiovascular disease). Thus, a need exists in the art for novel targeted therapies for the treatment and/or prevention of GPP.
SUMMARY OF THE INVENTION

[0009] The present invention addresses the above need by providing biotherapeutics, in particular antibodies, which bind to IL-36R and provide therapeutic or prophylactic therapy for GPP including acute GPP and the associated signs and symptoms such as GPP flares.

[00010] In one aspect, the invention relates to the treatment of GPP in a patient by administering to the patient two 900 mg intravenous (i.v.) doses of an anti-IL-antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[00011] In another aspect, the invention relates to the treatment of GPP
in a patient by administering to the patient a single 900 mg intravenous dose of an anti-IL-antibody, if the GPP symptoms persist, administering an additional 900 mg intravenous dose an anti-IL-36R antibody one week after the initial dose.

[00012] In one aspect, the invention relates to a method of treating generalized pustular psoriasis (GPP) flares in a patient, said method comprising administering to the patient two 900 mg intravenous (i.v.) doses of an anti-IL-36R antibody;
wherein the second dose is administered less than 2 weeks after the first dose.

[00013] In one aspect, the invention relates to a method of treating GPP
in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[00014] In one aspect, the invention relates to a method of reducing or alleviating signs and symptoms of an acute phase flare-up of GPP in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[00015] In one aspect, the invention relates to a method of reducing the severity and duration of GPP symptoms, said method comprising including administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[00016] In one aspect, the invention relates to a method of treating a skin disorder associated with GPP, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[00017] In one aspect, the invention relates to a method of preventing the recurrence of GPP flares in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[00018] In one aspect, the invention relates to a method of reducing pain by at least 10% in a patient with GPP, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[00019]
In one aspect, the invention relates to a method of improving the quality of life by at least 10% in a patient with moderate to severe GPP symptoms, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[00020]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of 2.

[00021]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2.

[00022]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before the administration of the first i.v. dose.

[00023]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 after the administration of the first i.v. dose.

[00024]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before and after the administration of the first i.v. dose.

[00025]
In an embodiment relating to any of the above aspects, the second dose is administered after 1 week but less than 2 weeks from the first dose.

[00026]
In one aspect, the invention relates to a method of treating a GPP patient with a GPPGA pustulation subscore of 2, said method comprising the steps of:
(a) administering to the patient a first 900 mg intravenous (I.V.) dose of an anti-antibody; (b) assessing the GPPGA pustulation subscore of the patient and if the GPPGA pustulation subscore of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

[00027]
In one aspect, the invention relates to a method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of 2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (i.v.) dose of an anti-IL-36R antibody; (b) assessing the GPPGA total score of the patient and if the GPPGA total score of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

[00028]
In one aspect, the invention relates to a method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (i.v.) dose of an anti-IL-36R antibody;
(b) assessing the GPPGA scores of the patient and if the GPPGA total score of 2 and the GPPGA pustulation subscore of 2 of the patient persist after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

[00029]
In an embodiment relating to any of the above aspects or embodiments, an optional third 900 mg i.v. dose of the anti-IL-36R antibody is administered at 2 to 12 weeks after the second i.v. dose.

[00030]
In an embodiment relating to any of the above aspects or embodiments, the two-dose administration achieves one or more of the following results:
(a) a Generalized Pustular Psoriasis Global Assessment (GPPGA) pustulation subscore of 0 indicating in one week after administering the second i.v. dose;
and/or (b) a GPPGA total score of 0 or 1 in one week after administering the second i.v. dose.

[00031]
In an embodiment relating to any of the above aspects or embodiments, the results are maintained for up to and at least 12 weeks following the administration of the second i.v. dose.

[00032] In an embodiment relating to any of the above aspects or embodiments, the method comprises administering to the patient a prophylactically effective amount of the anti-IL-36R antibody in one or more subcutaneous doses after the last i.v.
dose administered.

[00033] In an embodiment relating to any of the above aspects or embodiments, each of the one or more subcutaneous doses comprises 150 mg, 225 mg, 300 mg, 450 mg, or 600 mg of said anti-IL-36R antibody.

[00034] In an embodiment relating to any of the above aspects or embodiments, 1, 2, 3 or more subcutaneous doses are administered to the patient and wherein a first subcutaneous dose is administered after the last intravenous dose.

[00035] In an embodiment relating to any of the above aspects or embodiments, a first subcutaneous dose is administered 2 to 8 weeks, 4 to 6 weeks, 2 weeks, 4 weeks, 6 weeks or 8 weeks, 12 weeks, 16 weeks, 20 weeks after the last intravenous dose, and subsequent subcutaneous doses are administered at 2, 4, 6, 8, 10 or 12 weeks intervals after the first subcutaneous dose.

[00036] In an embodiment relating to any of the above aspects or embodiments, the patient remains in clinical remission as measured by a GPPGA total score of 0 or 1 for at least 12, 24, 36, 48, 60 or 72 weeks following the last i.v. or subcutaneous dose.

[00037] In an embodiment relating to any of the above aspects or embodiments, the anti-IL-36R antibody comprises: a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID NO:

35, 102, 103, 104, 105 106 or 140 (L-CDR2); the amino acid sequence of SEQ ID
NO:
44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).

[00038] In an embodiment relating to any of the above aspects or embodiments, the anti-IL-36R antibody comprises:

I. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 102 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
II. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 103 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
III. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 104 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
IV. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 105 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).

V. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 106 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
VI. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 140 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).

[00039] In an embodiment relating to any of the above aspects or embodiments, the anti-IL-36R antibody comprises:
(i) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 87;or (ii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 88; or (iii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 89; or (iv) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 87;or (V) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 88; or (vi) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 89; or (vii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 85; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 100; or (viii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 85; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:101; or (ix) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 86; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 100; or (x) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 86; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:101.

[00040] In an embodiment relating to any of the above aspects or embodiments, the anti-IL-36R antibody comprises:
i. a light chain comprising the amino acid sequence of SEQ ID NO:
115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 125; or ii. a light chain comprising the amino acid sequence of SEQ ID NO:
115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 126; or iii. a light chain comprising the amino acid sequence of SEQ ID NO:
115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 127; or iv. a light chain comprising the amino acid sequence of SEQ ID NO:
118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 125; or v. a light chain comprising the amino acid sequence of SEQ ID NO:
118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 126; or vi. a light chain comprising the amino acid sequence of SEQ ID NO:
118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 127; or vii. a light chain comprising the amino acid sequence of SEQ ID NO:
123; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 138; or viii. a light chain comprising the amino acid sequence of SEQ ID NO:
123; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 139; or ix. a light chain comprising the amino acid sequence of SEQ ID NO:
124; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 138.

[00041] In an embodiment relating to any of the above aspects or embodiments, the anti-IL-36R antibody is spesolimab

[00042] Additional features and advantages of the present invention will be set forth in the description below, and in part will be apparent from the description, or may be learned by practice of the subject technology. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to provide further explanation of the present invention as claimed.
BRIEF DESCRIPTION OF THE DRAWINGS

[00043] The accompanying drawings, which are included to provide further understanding of the present invention and are incorporated in and constitute a part of this specification, illustrate aspects of the subject technology and together with the description serve to explain the principles of the present invention.

[00044] FIG. 1 shows a CONSORT flow diagram for the trial described in Example 1. *Exclusion by other include trial completion, global recruitment target achieved and patients who did not flared within the 6-month screening period. tPatients were blinded to randomized treatment, but could be eligible to an open-label dose of spesolimab at Day 8. tPatients who did not complete 16 weeks residual period after last spesolimab dose were not rolled over into the OLE trial. Patients who did not continue in the OLE trial were to be followed for 16 weeks after the last dose of trial medication, which is the latest timepoint of trial medication given during the trial (e.g.
day 1, day 8 if OL spesolimab is given, rescue with OL spesolimab if given).
OL, open-label; OLE, open-label extension; SoC, standard of care.

[00045] FIG. 2 shows the trial design as described in Example 1. *Day 2-7:
Escape treatment (SoC) may be offered in case of disease worsening defined as worsening of clinical status or GPP skin and/or systemic symptoms as defined by the investigator.
tAfter day 8¨week 12: only one rescue dose with OL spesolimab is permitted if a patient who has previously achieved clinical response (GPPGA 0/1) to initial treatment, either with spesolimab or placebo at day 1, or escape medication or OL
spesolimab at day 8, experiences a recurrence of a GPP flare (2-point increase in the GPPGA score and the pustular component of GPPGA 2). Subsequent flares will be treated with SoC per physician's choice. tPatients who do not require rescue treatment with OL spesolimab are to be followed until week 12 (EoT) prior to entering into OLE trial. Patients who receive rescue treatment with OL spesolimab between weeks 2 and week 6 are to be followed until week 12 (EoT) prior to entering the OLE

trial. If at week 12 they qualify to enter the OLE trial, then the EoT will be considered for these patients. Patients who do not qualify to enter the OLE trial are to be followed for 16 weeks (EoT/week 16-28) after the last dose of trial medication, which is the latest timepoint of trial medication given during the trial (e.g. day 1, day 8 if OL
spesolimab is given, rescue with OL spesolimab if given). EoT, end of trial;
GPP, generalized pustular psoriasis; GPPGA, Generalized Pustular Psoriasis Physician Global Assessment; i.v., intravenous; OL, open-label, OLE, open-label extension; R, randomisation; SD, single dose; SoC, standard of care.

[00046] FIG. 3 shows the primary and key secondary outcomes. Panel A shows the percentage patients who had a Generalized Pustular Psoriasis Physician Global Assessment (GPPGA) pustulation subscore of 0 (complete pustule clearance) at week 1 after spesolimab or placebo treatment. Panel B shows the percentage patients who had global GPPGA score of 0 or 1 (clear or almost clear skin) at week 1 after a single intravenous dose of 900 mg spesolimab or placebo treatment. Cl, confidence interval.

[00047] FIG. 4 shows GPPGA Pustulation Subscore Over Time by Randomized Treatment at Day 1. Panels A and B show the proportion of patients by Generalized Pustular Psoriasis Physician Global Assessment (GPPGA) pustulation subscores at week 1 (day 8) and over time after receiving a single intravenous dose of placebo (panel A) or 900 mg spesolimab (panel B), respectively. Dashed line represents proportion of patients achieving GPPGA pustulation subscore of 0. *For this analysis, any use of escape medication, or open-label spesolimab at day 8, or rescue medication with open-label spesolimab at each timepoint were categorized separately (gray bars).

[00048] Fig. 5 shows GPPGA Total Score Over Time by Randomized Treatment at Day 1. Panels A and B show the proportion of patients by Generalized Pustular Psoriasis Physician Global Assessment (GPPGA) total score at week 1 (day 8) and over time after receiving a single intravenous dose of placebo (panel A) or 900 mg spesolimab (panel B), respectively. Dashed line represents proportion of patients achieving GPPGA Total score of 0 or 1. *For this analysis, any use of escape medication, or open-label spesolimab at day 8, or rescue medication with open-label spesolimab at each timepoint were categorized separately (gray bars).

[00049] FIG. 6 shows GPPGA Pustulation Subscore Over Time by Randomized Treatment at Day 1 and Open-Label Spesolimab Treatment at Day 8. At day 8, patients with a GPPGA total score 2 and a Generalized Pustular Psoriasis Physician Global Assessment (GPPGA) pustulation subscore 2 could receive an open-label spesolimab. GPPGA pustulation subscores from baseline are shown for: all patients randomized to spesolimab with or without receiving an open-label spesolimab dose on day 8 (panel A); patients randomized to spesolimab who did not receive an open-label spesolimab dose on day 8 (panel B); patients randomized to spesolimab who received an open-label spesolimab dose on day 8 (panel C); and placebo who received an open-label spesolimab dose on day 8 (panel D). Dashed line represents proportion of patients achieving GPPGA pustulation subscore of 0. *For this analysis, any use of escape medication, or open-label spesolimab at day 8, or rescue medication with open-label spesolimab at each timepoint were categorized separately (gray bars).

[00050] FIG. 7 shows GPPGA Total Score Over Time by Randomized Treatment at Day 1 and Open-Label Spesolimab Treatment at Day 8. At day 8, patients with GPPGA total score 2 and GPPGA pustulation subscore 2 could receive an open-label spesolimab. GPPGA total scores from baseline are shown for: all patients randomized to spesolimab with or without receiving an open-label spesolimab dose on day 8 (panel A); patients randomized to spesolimab who did not receive an open-label spesolimab dose on day 8 (panel B); patients randomized to spesolimab who received an open-label spesolimab dose on day 8 (panel C); and placebo who received an open-label spesolimab dose on day 8 (panel D). Dashed line represents proportion of patients achieving GPPGA Total score of 0 or 1. *For this analysis, any use of escape medication, or open-label spesolimab at day 8, or rescue medication with open-label spesolimab at each timepoint were categorized separately (gray bars).
GPP, Generalized Pustular Psoriasis; GPPGA, Generalized Pustular Psoriasis Physician Global Assessment.

[00051] Fig. 8 shows the Treatment Response in Patients Who Received Up to Two Doses of Spesolimab at Day 1 and Optional Dose at Day 8 in Spesolimab Arm.
Panel A shows the GPPASI results; panel B shows the GPPASI 75 results; panel C shows the Pain VAS results; panel D shows the DLQI results; panel E shows the Neutrophils Over Time in Patients With Neutrophils Above the Upper Limits of Normal at Baseline (7 x 109/L); panel F shows the C-Reactive Protein Over Time in Patients Who had Levels Above the Upper Limits of Normal at Baseline W 0 mg/L). The dataset includes observed cases in patients randomized to spesolimab who received up to two doses of spesolimab, including patients who received open-label spesolimab at day 8.
The arrowhead indicates the days of intravenous spesolimab administration. For this analysis, any values post open-label spesolimab at day 8 are used, but any values post use of escape medication, or rescue medication with spesolimab are not used and imputed as the worst outcome in the calculation of median and quartiles.
DLQI, Dermatology Life Quality Index; GPPASI, Generalized Pustular Psoriasis Area and Severity Index; GPPASI 75, 75% or greater improvement in the Psoriasis Area and Severity Index for Generalized Pustular Psoriasis; IQR, interquartile range;
pain VAS, Pain visual analog scale.

[00052] FIG. 9 shows change from baseline in GPPGA scores (including GPPGA

pustulation subscore and GPPGA total score) in three patients after receiving a first and a second dose of spesolimab on days 1 and 8, respectively. Panel A shows the changes in GPPGA scores (as well as skin erythema, scaling/crusting) in patient 1250001012 after receiving a first intravenous (i.v.) dose of 900 mg spesolimab and second i.v. dose of 900 mg spesolimab after 1 week. Panel B shows the changes in GPPGA scores (as well as skin erythema, scaling/crusting) in patient after receiving a first i.v. dose of 900 mg spesolimab and second i.v. dose of 900 mg spesolimab after 1 week. Panel C shows the changes in GPPGA scores (as well as skin erythema, scaling/crusting) in patient 1458001002 after receiving a first i.v. dose of 900 mg spesolimab and second i.v. dose of 900 mg spesolimab after 1 week.

[00053] FIG. 10 shows the PRO survey for PSS, Pain VAS, FACIT-Fatigue, and DLQI scores. High total scores indicate a large impairment or intense severity, except for FACIT-Fatigue, for which higher scores represent less fatigue. DLQI, Dermatology Life Quality Index; FACIT-Fatigue, Functional Assessment of Chronic Illness Therapy¨Fatigue; PRO, patient-reported outcome; PSS, Psoriasis Symptom Scale;
VAS, visual analogue scale.

[00054] FIG. 11 shows the absolute change from baseline in PRO scores over time.
Panel A shows the change from baseline in Pain VAS scores, Panel B shows the change from baseline in PSS scores, Panel C shows the change from baseline in FACIT-Fatigue scores, Panel D shows the change from baseline in DLQI scores.
Efficacy results for all randomised patients using the intention-to-treat analysis showed that all four PROs continued to improve over time; however, no statistical significance versus placebo was observed during Week 1. The placebo curve begins to converge with the spesolimab curve after administration of OL spesolimab at Day 8. Cl, confidence interval; DLQI, Dermatology Life Quality Index; FACIT-Fatigue, Functional Assessment of Chronic Illness Therapy¨Fatigue; IV, intravenous; OL, open-label; PRO, patient-reported outcome; PSS, Psoriasis Symptom Scale; VAS, visual analogue scale.

[00055] FIG. 12 shows the distribution of maximum ADA titers in female and male patients with GPP after i.v. administration of spesolimab.
DETAILED DESCRIPTION OF THE INVENTION
[00050] In the following detailed description, numerous specific details are set forth to provide a full understanding of the present invention. It will be apparent, however, to one ordinarily skilled in the art that the subject technology may be practiced without some of these specific details. In other instances, well-known structures and techniques have not been shown in detail so as not to obscure the present invention.
[00051] A phrase such as "an aspect" does not imply that such aspect is essential to the present invention or that such aspect applies to all configurations of the subject technology. A disclosure relating to an aspect may apply to all configurations, or one or more configurations. An aspect may provide one or more examples of the disclosure. A phrase such as "an aspect" may refer to one or more aspects and vice versa. A phrase such as "an embodiment" does not imply that such embodiment is essential to the subject technology or that such embodiment applies to all configurations of the subject technology. A disclosure relating to an embodiment may apply to all embodiments, or one or more embodiments. An embodiment may provide one or more examples of the disclosure.
[00052] The inventors have surprisingly discovered inter alia that the interleukin-36 pathway inhibition with a single dose of a humanized anti-interleukin-36R
(anti-IL-36R) monoclonal antibody of the present invention resulted in the rapid and sustained remission of clinical symptoms in patients with acute generalized pustular psoriasis and that no recurrence of GPP flares were observed in 20 weeks after the single dose administration.
[00053] The invention therefore relates to compositions and methods for treating and/or prophylaxis of GPP and its signs and symptoms. More specifically, the invention relates to compositions and methods for treating and/or prophylaxis of moderate to severe GPP, acute GPP, chronic GPP, and/or GPP flares in a mammal with an anti-IL36R antibody or an antigen-binding fragment thereof of the present invention. The compositions and methods include administering to the mammal a therapeutically effective amount of an anti-IL-36R antibody or an antigen-binding fragment thereof, wherein the anti-IL-36R antibody is administered in one intravenous dose. In an embodiment, the anti-IL-36R antibody is administered in one or more intravenous doses which is/are optionally followed by one or more subcutaneous doses.
[00054] Without wishing to be bound by this theory it is believed that anti-antibodies or antigen-binding fragments thereof bind to human anti-IL-36R and thus interfere with the binding of IL-36 agonists, and in doing so block at least partially the signaling cascade from the IL-36R to inflammatory mediators. The anti-IL36R
antibodies of the present invention are disclosed in U.S. Patent No. 9,023,995 or W02013/074569, the entire content of each of which is incorporated herein by reference.
[00055] Acute GPP flares of varying severity occur in most patients and may be idiopathic or triggered by external stimuli, such as infection, corticosteroid use or withdrawal, stress or pregnancy. Moderate or severe GPP flares cause significant morbidity and mortality due to tender, painful skin lesions, extreme fatigue, high fever, peripheral blood neutrophilia and acute phase response and sepsis. The acute phase is associated with a mean duration of hospitalization of 10 days (range 3-44 days).
The observed mortality rate of 7% reported in a retrospective study with 102 GPP
cases seen in a tertiary hospital in Johor, Malaysia is likely an underestimate as not all GPP patients were included in the study. Mortality rates are also likely underestimated due to lack of identifying the cause of death as GPP and are largely driven by infectious complications and extra-cutaneous organ manifestations such as renal, hepatic, respiratory and cardiac failure. After responding to treatment or spontaneous flare cessation, it is estimated that up to 50% of patients may suffer from chronic GPP characterized by persistent erythema and scaling that may also include joint symptoms.

[00056] Based on the limitations described above, current therapeutic options are not suitable for life-long treatment and do not provide sustained responses in most patients. Therefore, there is a high need to develop (i) a highly effective treatment with rapid onset of action for patients presenting with an acute GPP flare; and (ii) to develop an effective treatment of chronic GPP, which reliably prevents the occurrence of flares and is safe and tolerable for lifelong treatment.

[00057] The classic presentation of GPP flares as described by von Zumbusch is strongly correlated with polymorphisms in the 1L36-R signaling pathway.
Individuals with loss-of-function mutations of the IL36RN gene which encodes an endogenous IL36R antagonist (IL-36RN) have dramatically higher incidence of GPP, indicating that uncontrolled upregulation of IL36 signaling due to defective IL36RN antagonism leads to the inflammatory episodes observed in GPP. Genetic human studies have demonstrated the occurrence of GPP clusters in families with a loss of function mutation in IL36RN, which results in uncontrolled IL36R signaling. Mutations in other genes linked to the IL36 pathway such as CARD14 also lead to GPP. A recently published gene expression study indicates sustained activation of IL-1 and IL-36 in GPP, inducing neutrophil chemokine expression, infiltration, and pustule formation, suggesting that the IL-1/ IL-36 inflammatory axis is a potent driver of disease pathology in GPP. Moreover, a recent meta-analysis investigated 233 published GPP
cases. They found that 49 (21.0%) of 233 cases carried recessive IL36RN
alleles.
Those 49 recessive IL36RN alleles defined a GPP phenotype characterized by early onset and high risk of systemic inflammation.

[00058] IL36R is a cell surface receptor involved in inflammatory responses in skin and gut. It is a novel member of the !UR family that forms a heterodimeric complex with the !UR accessory protein. The heterodimeric IL36R system with stimulating (IL36a, 1L3613, IL36y) and inhibitory ligands (IL36Ra) shares a number of structural and functional similarities to other members of the IL1/1L1R family, such as IL1, IL18 and IL33 (R17-3602). All IL1 family members (IL1a, IL113, IL18, IL36a, 1L3613, IL36y, and IL38) signal through a unique, cognate receptor protein which, upon ligand binding, recruits the common IL1RacP subunit and activates NFkB and MAP kinase pathways in receptor-positive cell types. In human skin tissues, IL36R is expressed in keratinocytes, dermal fibroblasts and infiltrating myeloid cells. IL36R
activation in skin tissue drives the production of inflammatory mediators (e.g. CCL20, MIP-113, TNF-a, IL12, IL17, IL23, TGF13) and modulates the expression of tissue remodeling genes (e.g. MMPs, TGF13). Therefore, the link between GPP and mutations in the is somewhat analogous to the well-established neonatal onset of sterile multifocal osteomyelitis, periostitis, and pustulosis caused by absence of interleukin-1¨receptor antagonist. In this case, absence of the receptor antagonist allows unopposed action of interleukin-1, resulting in life-threatening systemic inflammation with skin and bone involvement. These clinical features responded to empirical treatment with the recombinant interleukin-1¨receptor antagonist anakinra.
I. Definitions

[00059] The term "about" shall generally mean an acceptable degree of error or variation for the quantity measured given the nature or precision of the measurements.
Typical, exemplary degrees of error or variation are within 5% or within 3% or within 1% of a given value or range of values. For example, the expression of "about 100"
includes 105 and 95 or 103 and 97 or 101 and 99, and all values in between (e.g., 95.1, 95.2, etc. for range of 95-105; or 97.1, 97.2, etc. for the range of 97-103; 99.1, 99.2, etc. for the range of 99-101). Numerical quantities given herein are approximates unless stated otherwise, meaning that the term "about" can be inferred when not expressly stated.

[00060] A "pharmaceutical composition" refers in this context to a liquid or powder preparation which is in such form as to permit the biological activity of the active ingredient(s) to be unequivocally effective, and which contains no additional components which are significantly toxic to the subjects to which the composition would be administered. Such compositions are sterile. A "powder" refers to a freeze-dried or lyophilized or a spray-dried pharmaceutical composition for parenteral use.
The powder is reconstituted or dissolved typically in water. Lyophilisation is a low temperature dehydration process which involves freezing the product, lowering pressure, then removing the ice by sublimation. Freeze drying results in a high quality product because of the low temperature used in processing. For a well-developed lyophilized formulation, the shape and appearance of the product is maintained over time and the quality of the rehydrated product is excellent. Spray drying is another method of producing a dry powder from a liquid or slurry by rapidly drying with a hot gas and with the goal of achieving a consistent particle size distribution.

[00061] As used herein, the terms "intravenous dose", "subcutaneous dose,"
in addition to their ordinary meanings, refer to the temporal sequence of administration of the anti-IL-36R antibody. Thus, the "intravenous dose" is the dose which is administered at the beginning of the treatment regimen (also referred to as the "baseline dose"); it may also be referred to as an "initial dose" or "induction dose." The "subcutaneous dose" is the dose which is administered after the intravenous dose, which may also be referred to as a "subsequent dose" or "maintenance dose."
The intravenous, subcutaneous doses may all contain the same amount of anti-IL-36R

antibody or an antigen binding fragment thereof, but generally may differ from one another in terms of the amount of the antibody administered or the frequency of administration. In an embodiment, the intravenous dose is equal or larger than the subcutaneous dose. An "intravenous dose" which may be interchangeably referred to as an "initial dose" or "induction dose" can be a single dose or, alternatively, a set of doses. The subcutaneous dose which may also be referred to as a "subsequent dose" or "maintenance dose" can be a single dose or, alternatively, a set of doses for administration.

[00062] In certain embodiments, the amount of the anti-IL-36R antibody contained in the induction/initial/intravenous and maintenance/subsequent/subcutaneous doses varies from one another during the course of treatment. In certain embodiments, the one or more initial/induction/intravenous doses each comprise a first amount of the antibody or antigen-binding fragment thereof and the one or more maintenance/subsequent/subcutaneous doses each comprise a second amount of the antibody or antigen-binding fragment thereof. In some embodiments, the first amount of antibody or fragment thereof is 1.5x, 2x, 2.5x, 3x, 3.5x, 4x, or 5x the second or subsequent amount of the antibody or antigen-binding fragment thereof. In certain embodiments, one or more (e.g., 1, 2, 3, 4, or 5 or more) initial doses are administered at the beginning of the treatment regimen as "loading doses" or "leading doses"
followed by subsequent doses that are administered on a less frequent basis (e.g., "maintenance doses"). In one embodiment, the intravenous dose, the induction dose or the initial dose is about 210 mg, 300 mg, 350 mg, 450 mg, 600 mg, 700 mg, mg, 800 mg, 850 mg or 900 mg of the anti-IL-36R antibody. In one embodiment, the subcutaneous dose, the maintenance dose or the subsequent dose is about 150, mg or 300 mg. In another embodiment, the subcutaneous dose or maintenance or subsequent dose is administered at least two weeks following the intravenous, induction or initial dose.

[00063] The terms, "antibody", "anti-IL-36R antibody", "humanized anti-IL-antibody", "humanized anti-IL-36R epitope antibody", and "variant humanized anti-IL-36R epitope antibody" specifically encompass monoclonal antibodies (including full length monoclonal antibodies), polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies), antibodies with minor modifications such as N- and/or C-terminal truncation, and antibody fragments such as variable domains and other portions of antibodies that exhibit a desired biological activity, e.g., IL-36R binding.

[00064] The term "monoclonal antibody" (mAb) refers to an antibody that is highly specific, being directed against a single antigenic determinant, an "epitope".

Therefore, the modifier "monoclonal" is indicative of antibodies directed to the identical epitope and is not to be construed as requiring production of the antibody by any particular method. It should be understood that monoclonal antibodies can be made by any technique or methodology known in the art; including e.g., the hybridoma method ( Kohler et al., 1975, Nature 256:495), or recombinant DNA methods known in the art (see, e.g., U.S. Pat. No. 4,816,567), or methods of isolation of monoclonal recombinantly produced using phage antibody libraries, using techniques described in Clackson et al., 1991, Nature 352: 624-628, and Marks et al., 1991, J. Mol.
Biol.
222: 581-597.

[00065] The term "monomer" refers to a homogenous form of an antibody. For example, for a full-length antibody, monomer means a monomeric antibody having two identical heavy chains and two identical light chains.

[00066] Chimeric antibodies consist of the heavy and light chain variable regions of an antibody from one species (e.g., a non-human mammal such as a mouse) and the heavy and light chain constant regions of another species (e.g., human) antibody and can be obtained by linking the DNA sequences encoding the variable regions of the antibody from the first species (e.g., mouse) to the DNA sequences for the constant regions of the antibody from the second (e.g. human) species and transforming a host with an expression vector containing the linked sequences to allow it to produce a chimeric antibody. Alternatively, the chimeric antibody also could be one in which one or more regions or domains of the heavy and/or light chain is identical with, homologous to, or a variant of the corresponding sequence in a monoclonal antibody from another immunoglobulin class or isotype, or from a consensus or germline sequence. Chimeric antibodies can include fragments of such antibodies, provided that the antibody fragment exhibits the desired biological activity of its parent antibody, for example binding to the same epitope (see, e.g., U.S. Pat. No. 4,816,567;
and Morrison et al., 1984, Proc. Natl. Acad. Sci. USA 81:6851-6855).

[00067] The term "intravenous infusion" refers to introduction of an agent into the vein of an animal or human patient over a period of time greater than approximately 15 minutes, generally between approximately 30 to 90 minutes.

[00068] The term "intravenous bolus" or "intravenous push" refers to drug administration into a vein of an animal or human such that the body receives the drug in approximately 15 minutes or less, generally 5 minutes or less.

[00069] The term "subcutaneous administration" refers to introduction of an agent under the skin of an animal or human patient, preferable within a pocket between the skin and underlying tissue, by relatively slow, sustained delivery from a drug receptacle. Pinching or drawing the skin up and away from underlying tissue may create the pocket.

[00070] The terms "treatment" and "therapy" and the like, as used herein, are meant to include therapeutic as well as prophylactic, or suppressive measures for a disease or disorder leading to any clinically desirable or beneficial effect, including but not limited to alleviation or relief of one or more symptoms, regression, slowing or cessation of progression of the disease or disorder. Thus, for example, the term treatment includes the administration of an agent prior to or following the onset of a symptom of a disease or disorder thereby preventing or removing one or more signs of the disease or disorder. As another example, the term includes the administration of an agent after clinical manifestation of the disease to combat the symptoms of the disease. Further, administration of an agent after onset and after clinical symptoms have developed where administration affects clinical parameters of the disease or disorder, such as the degree of tissue injury or the amount or extent of metastasis, whether or not the treatment leads to amelioration of the disease, comprises "treatment" or "therapy" as used herein. Moreover, as long as the compositions of the invention either alone or in combination with another therapeutic agent alleviate or ameliorate at least one symptom of a disorder being treated as compared to that symptom in the absence of use of the humanized anti-IL-36R antibody composition, the result should be considered an effective treatment of the underlying disorder regardless of whether all the symptoms of the disorder are alleviated or not.

[00071] The term "prophylactically effective amount" is used to refer to an amount effective, at dosages and for periods of time necessary, to achieve the desired prophylactic result. Typically, a prophylactic dose is used in subjects prior to the onset of a GPP flare and/or prior to the onset of symptoms of GPP such as to prevent or inhibit the occurrence of acute flares. In an embodiment, a subcutaneous dose as contemplated herein is a prophylactic dose that is used in a patient with acute GPP, after the intravenous dose, to prevent a possible recurrence of the GPP flares in the patient.
II. Antibodies

[00072] The anti-IL36R antibodies of the present invention are disclosed in U.S.
Patent No. 9,023,995 or W02013/074569, the entire content of each of which is incorporated herein by reference.

[00073] In one aspect, described and disclosed herein are anti-IL-36R
antibodies, in particular humanized anti-IL-36R antibodies, and compositions and articles of manufacture comprising one or more anti-IL-36R antibody, in particular one or more humanized anti-IL-36R antibody of the present invention. Also described are binding agents that include an antigen-binding fragment of an anti-IL-36 antibody, in particular a humanized anti-IL-36R antibody.
Mode of action

[00074] An anti-IL-36R antibody of the present invention is a humanized antagonistic monoclonal IgG1 antibody that blocks human IL36R signaling.
Binding of an anti-IL-36R antibody of the present invention to IL36R is anticipated to prevent the subsequent activation of IL36R by cognate ligands (IL36 a, 13 and y) and downstream activation of pro-inflammatory and pro-fibrotic pathways with the aim to reduce epithelial cell/ fibroblast/ immune cell-mediated inflammation and interrupt the inflammatory response that drives pathogenic cytokine production in generalized pustular psoriasis (GPP). As provided herein, an anti-IL-36R antibody of the present invention has been tested and proved to be effective in treating patients with acute Generalized Pustular Psoriasis (GPP), a severe inflammatory skin disease driven by uncontrolled IL36 activity.

[00075] IL-36R is also known as IL-1RL2 and IL-1Rrp2. It has been reported that agonistic IL-36 ligands (a, 13, or y) initiate the signaling cascade by engaging the IL-36 receptor which then forms a heterodimer with the IL-1 receptor accessory protein (IL-1RAcP). IL-36 antagonist ligands (IL-36RA/IL1F5, IL-38/ILF10) inhibit the signaling cascade.

[00076] Variable regions and CDRs of representative antibodies of the present invention are disclosed below:
Anti-IL-36R Mouse Antibody Sequences

[00077] Variable regions and CDRs of representative mouse lead antibodies of the present invention (mouse leads) are shown below:
Light Chain Variable Region (VK) Amino Acid Sequences >33D10612vK Protein (antibody 33D10) QIVLTQSPAIMSASLGERVTMTCTASSSVSSSYLHWYQKKPGSSPKLWVYSTSNLASGVPVRF
SGSGSGTSYSLTISSMEAEDAATYYCHQHHRSPVTFGSGTKLEMK (SEQ ID NO: 1) >17208612 vK protein (antibody 17208) DIQMTQSPASQSASLGESVTFTCLASQTIGTWLAWYQQRPGKSPQLLIYAATSLADGVPSRFS
GSGSGTQFSFNIRSLQAEDFASYYCQQVYTTPLTFGGGTKLEIK (SEQ ID NO: 2) >67E7E8 vK protein (antibody 67E7) D IQMTQSPASQSASLG ESVTFTCLASQTIGTWLGWYQQKPG KSPQLLIYRSTTLADGVPS RFS
GSGSGTKFSFKISSLQAADFASYYCQQLYSAPYTFGGGTKLEIR (SEQ ID NO: 3) >7808D1 vK Protein (antibody 7808) DVLLTQTPLSLPVSLGDQASISCRSSQNIVHSNGNTYLQWYLQKPGQSPKLLIYKVSNRFSGVP
DRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPFTFGAGTKLELK (SEQ ID NO: 4) >81A1 D1 vK Protein (antibody 81A1) DIQMTQTTSSLSASLGDRVTISCRASQDIYKYLNWYQQKPDGTLKLLIYYTSGLHSGVPSRFSG
SGSGTDFSLTISNLEPEDIATYFCQQDSKFPWTFGGDTKLEIK (SEQ ID NO: 5) >81134E11 vK Protein (antibody 81134) QIVLTQSPAIMSASLGERVTMTCTASSSVSSSYFHWYQQKPGSSPKLWIYRTSNLASGVPGRF
SGSGSGTSYSLTISSMEAEDAATYYCHQFHRSPLTFGAGTKLELK (SEQ ID NO: 6) >7305010 vK protein (antibody 7305) DIVMTQSQKFLSTSVGVRVSVTCKASQDVGTNVLWYQQKIGQSPKPLIYSASYRHSGVPDRFT
GSGSGTDFTLIISNVQSEDLAEYFCQQYSRYPLTFGPGTKLELK (SEQ ID NO: 7) >73F6F8 vK protein (antibody 73F6) D IVMTQSQKFLSTSVGVRVSVTCKASQDVGTNVLWYQQKIGQSP KALIYSASYRHSGVP DRFT
GSGSGTDFTLIITNVQSEDLAEYFCQQYSRYPLTFGPGTKLELK (SEQ ID NO: 8) >76E10E8 vK protein (antibody 76E10) DIVMTQSQKFMSATVGGRVNITCKASQNVGRAVAWYQQKPGQSPKLLTHSASNRYTGVPDRF
TGSGSGTDFTLTITNMQSEDLADYFCQQYSSYPLTFGAGTKLDLK (SEQ ID NO: 9) >89A12138 vK protein (antibody 89Al2) DIQMTQSPASQSASLGESVTFSCLASQTIGTWLGWYQQKPGKSPQLLIYRATSLADGVPSRFS
GSGSGTNFSFKISSLQAEDLASYYCQQLYSGPYTFGGGTKLEIR (SEQ ID NO: 10) Heavy Chain Variable Region (VH) Amino Acid Sequences >33D10B12vH Protein (antibody 33D10) QVQLQQSGTELLKPGASVKLSCKASGNTVTSYWMHWVKQRPGQGLEWIGEILPSTGRTNYNE
NFKGKAMLTVDKSSSTAYMQLSSLASEDSAVYYCTIVYFGNPWFAYWGQGTLVTVSA (SEQ
ID NO: 11) >17208612 vH protein (antibody 17208) EVQLQQSGPELVKPGASVKLSCKASGYTFTDNYMNWVRQSHGKSLEWIGRVNPSNGDTKYN
QNFKGKATLTVDKSLSTAYMQLNGLTSEDSAVYYCGRTKNFYSSYSYDDAMDYWGQGTSVTV
SS (SEQ ID NO: 12) >67E7E8 vH protein (antibody 67E7) EVQLQQSGAEFVRPGASVKFSCTASGFNIKDDYIHWVRQRPEQGLEWVGRIDPANGNTKYAP
KFQDKATITADTSSNTAYLQLSSLTSEDTAVYYCAKSFPNNYYSYDDAFAYWGQGTLVTVSA
(SEQ ID NO: 13) >7808D1 vH Protein (antibody 7808) QVQLKESGPVLVAPSQSLSITCTVSGFSLTKFGVHWIRQTPGKGLEWLGVIWAGGPTNYNSAL
MSRLTISKDISQSQVFLRIDSLQTDDTAMYYCAKQIYYSTLVDYWGQGTSVTVSS (SEQ ID NO:
14) >81A1D1 vH Protein (antibody 81A1) QVQLKESGPGLVAPSQSLFITCTVSGFSLSSYEINWVRQVPGKGLEWLGVIWTGITTNYNSALI
SRLSISKDNSKSLVFLKMNSLQTDDTAIYYCARGTGTGFYYAMDYWGQGTSVTVSS (SEQ ID
NO: 15) >8164E11 vH Protein (antibody 8164) QVQLQQPGADFVRPGASMRLSCKASGYSFTSSWIHWVKQRPGQGLEWIGEINPGNVRTNYN
ENFRNKATLTVDKSSTTAYMQLRSLTSADSAVYYCTVVFYGEPYFPYWGQGTLVTVSA (SEQ
ID NO: 16) >7305010 vH Protein (antibody 7305) QVQLKESGPGLVAPSQSLSITCTVSGFSLTNYAVHWVRQFPGKGLEWLGVIWSDGSTDFNAP
FKSRLSINKDNSKSQVFFKMNSLQIDDTAIYYCARKGGYSGSWFAYWGQGTLVTVSA (SEQ ID
NO: 17) >73F6F8 vH protein (antibody 73F6) QVQLKESGPGLVAPSQSLSITCTVSGFSLTNYAVHWVRQFPGKGLEWLGVIWSDGSTDYNAP
FKSRLSINKDNSKSQVFFKMNSLQTDDTAIYYCARKGGYSGSWFAYWGQGTLVTVSA (SEQ
ID NO: 18) >76E10E8 vH protein (antibody 76E10) QVQLKESGPVLVAPSQSLSITCTVSGFSLTNYGVHWVRQPPGKGLEWLGVIWPVGSTNYNSA
LMSRLSIHKDNSKSQVFLRMNSLQTDDTAIYYCAKMDWDDFFDYWGQGTTLTVSS(SEQ ID
NO: 19) >89Al2B8 vH Protein (antibody 89Al2) EVQLQQSGAELVRPGASVRLSCTASGFNIKDDYIHWVRQRPKQGLEWLGRIDPANGNTKYDP
RFQDKATITADTSSNTAYLHLSSLTSEDTAVYYCAKSFPDNYYSYDDAFAYWGQGTLVTVSA
(SEQ ID NO: 20) Light chain CDR-1 (L-CDR1) Amino Acid Sequences >33D10G1 L-CDR1 TASSSVSSSYLH (SEQ ID NO: 21) >172C8B12 L-CDR1 LASQTIGTWLA (SEQ ID NO: 22) >67E7E8 L-CDR1 LASQTIGTWLG (SEQ ID NO: 23) >78C8D1 L-CDR1 RSSQNIVHSNGNTYLQ (SEQ ID NO: 24) >81A1D1 L-CDR1 RASQDIYKYLN (SEQ ID NO: 25) >81134E11 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >73C5C10 L-CDR1 KASQDVGTNVL (SEQ ID NO: 27) >73F6F8 L-CDR1 KASQDVGTNVL (SEQ ID NO: 27) >76E10E8 L-CDR1 KASQNVGRAVA (SEQ ID NO: 28) >89Al2B8 L-CDR1 LASQTIGTWLG (SEQ ID NO: 29) Light chain CDR-2 (L-CDR2) Amino Acid Sequences >33D10612 L-CDR2 STSNLAS (SEQ ID NO: 30) >172C8B12 L-CDR2 AATSLAD ( SEQ ID NO: 31) >67E7E8 L-CDR2 RSTTLAD (SEQ ID NO: 32) >78C8D1 L-CDR2 KVSNRFS (SEQ ID NO: 33) >81A1D1 L-CDR2 YTSGLHS (SEQ ID NO: 34) >81134E11 L-CDR2 RTSNLAS (SEQ ID NO: 35) >73C5C10 L-CDR2 SASYRHS (SEQ ID NO: 36) >73F6F8 L-CDR2 SASYRHS (SEQ ID NO: 36) >76E10E8 L-CDR2 SASNRYT (SEQ ID NO: 37) >89Al2B8 L-CDR2 RATSLAD (SEQ ID NO: 38) Light chain CDR-3 (L-CDR3) Amino Acid Sequences >33D10612 L-CDR3 HQHHRSPVT (SEQ ID NO: 39) >172C8B12 L-CDR3 QQVYTTPLT (SEQ ID NO: 40) >67E7E8 L-CDR3 QQLYSAPYT (SEQ ID NO: 41) >78C8D1 L-CDR3 FQGSHVPFT (SEQ ID NO: 42) >81A1D1 L-CDR3 QQDSKFPWT (SEQ ID NO: 43) >81134E11 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >73C5C10 L-CDR3 QQYSRYPLT (SEQ ID NO: 45) >73F6F8 L-CDR3 QQYSRYPLT (SEQ ID NO: 45) >76E10E8 L-CDR3 QQYSSYPLT (SEQ ID NO: 46) >89Al2B8 L-CDR3 QQLYSGPYT (SEQ ID NO: 47) Heavy chain CDR-1 (H-CDR1) Amino Acid Sequences >33D10612 H-CDR1 GNTVTSYWMH (SEQ ID NO: 48) >172C8B12 H-CDR1 GYTFTDNYMN (SEQ ID NO: 49) >67E7E8 H-CDR1 GFNIKDDYIH (SEQ ID NO: 50) >78C8D1 H-CDR1 GFSLTKFGVH (SEQ ID NO: 51) >81A1D1 H-CDR1 GFSLSSYEIN (SEQ ID NO: 52) >81134E11 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >73C5C10 H-CDR1 GFSLTNYAVH (SEQ ID NO: 54) >73F6F8 H-CDR1 GFSLTNYAVH (SEQ ID NO: 54) >76E10E8 H-CDR1 GFSLTNYGVH (SEQ ID NO: 55) >89Al2B8 H-CDR1 GFNIKDDYIH (SEQ ID NO: 56) Heavy chain CDR-2 (H-CDR2) Amino Acid Sequences >33D10612 H-CDR2 EILPSTGRTNYNENFKG (SEQ ID NO: 57) >172C8B12 H-CDR2 RVNPSNGDTKYNQNFKG (SEQ ID NO: 58) >67E7E8 H-CDR2 RIDPANGNTKYAPKFQD (SEQ ID NO: 59) >78C8D1 H-CDR2 VIWAGGPTNYNSALMS (SEQ ID NO: 60) >81A1D1 H-CDR2 VIWTGITTNYNSALIS (SEQ ID NO: 61) >81134E11 H-CDR2 EINPGNVRTNYNENF (SEQ ID NO: 62) >73C5C10 H-CDR2 VIWSDGSTDFNAPFKS (SEQ ID NO: 63) >73F6F8 H-CDR2 VIWSDGSTDYNAPFKS (SEQ ID NO: 64) >76E10E8 H-CDR2 VIWPVGSTNYNSALMS (SEQ ID NO: 65) >89Al2B8 H-CDR2 RIDPANGNTKYDPRFQD (SEQ ID NO: 66) Heavy chain CDR-3 (H-CDR3) Amino Acid Sequences >33D10612 H-CDR3 VYFGNPWFAY (SEQ ID NO: 67) >172C8B12 H-CDR3 TKNFYSSYSYDDAMDY (SEQ ID NO: 68) >67E7E8 H-CDR3 SFPNNYYSYDDAFAY (SEQ ID NO: 69) >78C8D1 H-CDR3 QIYYSTLVDY (SEQ ID NO: 70) >81A1D1 H-CDR3 GTGTGFYYAMDY (SEQ ID NO: 71) >81134E11 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >73C5C10 H-CDR3 KGGYSGSWFAY (SEQ ID NO: 73) >73F6F8 H-CDR3 KGGYSGSWFAY (SEQ ID NO: 73) >76E10E8 H-CDR3 MDWDDFFDY (SEQ ID NO: 74) >89Al2B8 H-CDR3 SFPDNYYSYDDAFAY (SEQ ID NO: 75) Anti-IL-36R Mouse CDR Sequences A summary of the CDR sequences of the lead mouse antibodies is shown below:
Antibody H-CDR Sequences L-CDR Sequences 33D10 GNTVTSYWMH (H-CDR1) TASSSVSSSYLH (L-SEQ ID No: 48 CDR1) SEQ ID No: 21 EILPSTGRTNYNENFKG STSNLAS (L-CDR2) SEQ
(H-CDR2) SEQ ID No: 57 ID No: 30 VYFGNPWFAY (H-CDR3) HQHHRSPVT (L-CDR3) SEQ ID No: 67 SEQ ID No: 39 172C8 GYTFTDNYMN (H-CDR1) LASQTIGTWLA (L-CDR1) SEQ ID No: 49 SEQ ID No: 22 RVNPSNGDTKYNQNFKG AATSLAD (L-CDR2) SEQ
(H-CDR2) SEQ ID No: 58 ID No: 31 TKNFYSSYSYDDAMDY QQVYTTPLT (L-CDR3) (H-CDR3) SEQ ID No: 68 SEQ ID No: 40 67E7 GFNIKDDYIH (H-CDR1) LASQTIGTWLG (L-CDR1) SEQ ID No: 50 SEQ ID No: 23 RIDPANGNTKYAPKFQD RSTTLAD (L-CDR2) SEQ
(H-CDR2) SEQ ID No: 59 ID No: 32 SFPNNYYSYDDAFAY (H- QQLYSAPYT (L-CDR3) CDR3) SEQ ID No: 69 SEQ ID No: 41 78C8 GFSLTKFGVH (H-CDR1) RSSQNIVHSNGNTYLQ (L-SEQ ID No: 51 CDR1) SEQ ID No: 24 VIWAGGPTNYNSALMS KVSNRFS (L-CDR2) SEQ
(H-CDR2) SEQ ID No: 60 ID No: 33 QIYYSTLVDY (H-CDR3) FQGSHVPFT (L-CDR3) SEQ ID No: 70 SEQ ID No: 42 81A1 GFSLSSYEIN (H-CDR1) RASQDIYKYLN (L-CDR1) SEQ ID No: 52 SEQ ID No: 25 YTSGLHS (L-CDR2) SEQ
VIWTGITTNYNSALIS (H- ID No: 34 CDR2) SEQ ID No: 61 QQDSKFPWT (L-CDR3) SEQ ID No: 43 GTGTGFYYAMDY (H-CDR3) SEQ ID No: 71 81134 GYSFTSSWIH (H-CDR1) TASSSVSSSYFH (L-SEQ ID No: 53 CDR1) SEQ ID No: 26 RTSNLAS (L-CDR2) SEQ
EINPGNVRTNYNENF (H- ID No: 35 CDR2) SEQ ID No: 62 HQFHRSPLT (L-CDR3) SEQ ID No: 44 VFYGEPYFPY (H-CDR3) SEQ ID No: 72 73C5 GFSLTNYAVH (H-CDR1) KASQDVGTNVL (L-CDR1) SEQ ID No: 54 SEQ ID No: 27 VIWSDGSTDFNAPFKS (H- SASYRHS (L-CDR2) SEQ
CDR2) SEQ ID No: 63 ID No: 36 KGGYSGSWFAY (H- QQYSRYPLT (L-CDR3) CDR3) SEQ ID No: 73 SEQ ID No: 45 73F6 GFSLTNYAVH (H-CDR1) KASQDVGTNVL (L-CDR1) SEQ ID No: 54 SEQ ID No:27 VIWSDGSTDYNAPFKS SASYRHS (L-CDR2) SEQ
(H-CDR2) SEQ ID No: 64 ID No: 36 KGGYSGSWFAY (H- QQYSRYPLT (L-CDR3) CDR3) SEQ ID No: 73 SEQ ID No: 45 76E10 GFSLTNYGVH (H-CDR1) KASQNVGRAVA (L-CDR1) SEQ ID No: 55 SEQ ID No: 28 VIWPVGSTNYNSALMS SASNRYT (L-CDR2) SEQ
(H-CDR2) SEQ ID No: 65 ID No: 37 MDWDDFFDY (H-CDR3) QQYSSYPLT (L-CDR3) SEQ ID No: 74 SEQ ID No: 46 89Al2 GFNIKDDYIH (H-CDR1) LASQTIGTWLG (L-CDR1) SEQ ID No: 56 SEQ ID No: 29 RIDPANGNTKYDPRFQD RATSLAD (L-CDR2) SEQ
(H-CDR2) SEQ ID No: 66 ID No: 38 QQLYSGPYT (L-CDR3) SFPDNYYSYDDAFAY (H- SEQ ID No: 47 CDR3) SEQ ID No: 75 Anti-IL-36R Humanized Antibody Sequences

[00078] Human framework sequences were selected for the mouse leads based on the framework homology, CDR structure, conserved canonical residues, conserved interface packing residues and other parameters to produce humanized variable regions (see Example 5).

[00079] Representative humanized variable regions derived from antibodies and 73C5 are shown below.
Light Chain Variable Region (VK) Amino Acid Sequences >81B4vK32_3 vK protein EIVLTQSPGTLSLSPGERATMSCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSTLASGI
PDRFSGSGSGTDFTLTISRLEPEDAATYYCHQFHRSPLTFGQGTKLEIK (SEQ ID NO:
76) >81B4vK32 105 vK protein EIVLTQSPGTLSLSPGERATMSCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSILASGV
PDRFSGSGSGTDFTLTISRLEPEDFATYYCHQFHRSPLTFGQGTKLEIK (SEQ ID NO:
77) >81B4vK32 116 vK protein EIVLTQSPGTLSLSPGERATMSCTASSSVSSSYFHWYQQKPGQAPRLWIYRTSRLASG
VPDRFSGSGSGTDFTLTISRLEPEDAATYYCHQFHRSPLTFGQGTKLEIK (SEQ ID NO:
78) >81B4vK32 127 vK protein EIVLTQSPGTLSLSPGERATMTCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSRLASG
VPDRFSGSGSGTDFTLTISRLEPEDFAVYYCHQFHRSPLTFGQGTKLEIK (SEQ ID NO:
79) >81B4vK32 138 vK protein QIVLTQSPGTLSLSPGERATMTCTASSSVSSSYFHWYQQKPGQAPRLWIYRTSRLAS
GVPDRFSGSGSGTDFTLTISRLEPEDAATYYCHQFHRSPLTFGAGTKLEIK (SEQ ID
NO: 80) >81B4vK32 140 vK protein QIVLTQSPGTLSLSPGERVTMSCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSQLASG
IPDRFSGSGSGTDFTLTISRLEPEDAATYYCHQFHRSPLTFGQGTKLEIK (SEQ ID NO:
81) >81B4vK32 141 vK protein QIVLTQSPGTLSLSPGERATMTCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSKLASG
VPDRFSGSGSGTDFTLTISRLEPEDFATYYCHQFHRSPLTFGQGTKLEIK (SEQ ID NO:
82) >81 B4vK32 147 vK protein EIVLTQSPGTLSLSPGERATMSCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSHLASGI
PGRFSGSGSGTDFTLTISRLEPEDAAVYYCHQFHRSPLTFGQGTKLEIK (SEQ ID NO:
83) 1 >73C5vK39_2 vK protein E IVMTQSPATLSVSPGVRATLSCKASQDVGTNVLWYQQKPGQAP RPLIYSASYRHSG I
PDRFSGSGSGTEFTLTISSLQSEDFAEYFCQQYSRYPLTFGQGTKLEIK (SEQ ID NO:
84) >73C5vK39_7 vK protein E IVMTQSPATLSVSPGVRATLSCKASQDVGTNVLWYQQKPGQAP RPLIYSASYRHSG I
PDRFSGSGSGTEFTLTISSLQSEDFAVYYCQQYSRYPLTFGQGTKLEIK (SEQ ID NO:
85) >73C5vK39 15 vK protein E IVMTQSPATLSVSPGVRATLSCKASQDVGTNVLWYQQKPGQAP RPLIYSASYRHSG I
PARFSGSGSGTEFTLTISSLQSEDFAEYYCQQYSRYPLTFGQGTKLEIK (SEQ ID NO:
86) Heavy Chain Variable Region (VH) Amino Acid Sequences >81B4vH33 49 vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWI HWVRQAPGQG LE WIG El N PG NV
RTNYN ENFRNKATMTVDTSISTAYM ELSRLRSDDTAVYYCAVVFYG E PYFPYWGQGT
LVTVSS (SEQ ID NO: 87) >81B4vH33 85T vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWI HWVRQRPGQG LE WIG El N PG NV
RTNYN ENFRN RVTMTVDTS ISTAYMELS RLRSDDTAVYYCTVVFYG E PYFPYWGQGT
LVTVSS (SEQ ID NO: 88) >81B4vH33 90 vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWIHWVKQAPGQG LEWMGE IN PG NV
RTNYNENFRNKVTMTVDTSISTAYMELSRLRSDDTAVYYCTVVFYGEPYFPYWGQGT
LVTVSS (SEQ ID NO: 89) >81B4vH33 93 vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWIHWVRQRPGQG LEWMGE IN PG NV
RTNYN ENFRN RATLTRDTS ISTAYME LSRLRSDDTAVYYCAVVFYG E PYFPYWGQGTL
VTVSS (SEQ ID NO: 90) >81B4vH50 22 vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWIHWVRQRPGQG LEWMGE ILPGVV
RTNYNENFRNKVTMTVDTSISTAYMELSRLRSDDTAVYYCTVVFYGEPYFPYWGQGT
LVTVSS (SEQ ID NO: 91) >81B4vH50 30 vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSW I HWVRQRPGQG LE WIG El N PGAV
RTNYN EN F RN RVTMTVDTSISTAYMELSRLRSDDTAVYYCTVVFYGE PYFPYWGQGT
LVTVSS (SEQ ID NO: 92) >81B4vH51 13 vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSW I HWVRQAPGQG LE WIG El N PG LVR
TNYN EN F RN KVTMTVDTS ISTAYME LS RL RSDDTAVYYCAVVFYG E PYF PYWGQGTL
VTVSS (SEQ ID NO: 93) >81B4vH51 15 vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSW I HWVRQAPGQG LE WIG El NPGAVR
TNYN EN F RN KVTMTVDTS ISTAYME LS RL RSDDTAVYYCAVVFYG E PYF PYWGQGTL
VTVSS (SEQ ID NO: 94) >81B4vH52 83 vH Protein QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSW I HWVRQAPGQG LE WIG El NPGSVR
TNYN EN F RN KATMTVDTS ISTAYME LS RL RSDDTAVYYCAVVFYG E PYF PYWGQGTL
VTVSS (SEQ ID NO: 95) >73C5vH46_4 vH Protein QVQLQESG PG LVKPSETLSITCTVSG FSLTDYAVHW I RQP PG KG LEW IGVIWSDGSTD
YNA PFKS RVT I N KDTS KSQVSFKMSSVQAADTAVYYCARKGGYSGSWFAYWGQGTL
VTVSS (SEQ ID NO: 96) >73C5vH46 19 vH Protein QVQLQESG PG LVKPSETLSITCTVSG FSLTDYAVHW I RQP PG KG LEW IGVIWSDGSTD
YNAPFKSRVTISKDTSKNQVSLKMNSLTTDDTAVYYCARKGGYSGSWFAYWGQGTLV
TVSS (SEQ ID NO: 97) >73C5vH46_40 vH Protein QVQLQESG PG LVKPSETLSITCTVSG FSLTDYAVHW I RQP PG KG LEW IGVIWSDGSTD
YNAPFKSRVTISKDNSKSQVSLKMNSVTVADTAVYYCARKGGYSGSWFAYWGQGTL
VTVSS (SEQ ID NO: 98) >73C5vH47 65 vH Protein QVQLQESG PG LVKPSETLSITCTVSG FSLTDYAVHWVRQP PG KGLEWIGVIWSDGST
DYNAPFKSRVTISKDTSKNQVSFKLSSVTVDDTAVYYCARKGGYSGSWFAYWGQGTL
VTVSS (SEQ ID NO: 99) >73C5vH47 77 vH Protein QVQLQESGPGLVAPSETLSLTCTVSGFSLTDYAVHWIRQFPGKGLEWIGVIWSDGSTD
FNAPFKSRVTISKDTSKNQVSFKLSSVTTDDTAVYYCARKGGYSGSWFAYWGQGTLV
TVSS (SEQ ID NO: 100) >73C5vH58 91 vH Protein QVQLQESGPGLVKPSETLSITCTVSGFSLTDYAVHWIRQPPGKGLEWIGVIWSDGSTD
YNAPFKSRVTISKDNSKSQVSFKMSSVTADDTAVYYCARKGGYSGSWFAYWGQGTL
VTVSS (SEQ ID NO: 101) The CDR sequences from the humanized variable regions derived from antibodies 8164 and 73C5 shown above are depicted below.
L-CDR1 Amino Acid Sequences >81B4vK32_3 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >81B4vK32_105 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >81B4vK32_116 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >81B4vK32_127 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >81B4vK32_138 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >81B4vK32_140 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >81B4vK32_141 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >81B4vK32_147 L-CDR1 TASSSVSSSYFH (SEQ ID NO: 26) >73C5vK39_2 L-CDR1 KASQDVGTNVL (SEQ ID NO: 27) >73C5vK39_7 L-CDR1 KASQDVGTNVL (SEQ ID NO: 27) >73C5vK39 15 L-CDR1 KASQDVGTNVL (SEQ ID NO: 27) L-CDR2 Amino Acid Sequences >81B4vK32 3 L-CDR2 (SEQ ID 102) RTSTLAS
>81B4vK32 105 L-CDR2 (SEQ ID 103) RTSILAS
>81B4vK32 116 L-CDR2 (SEQ ID 104) RTSRLAS
>81B4vK32 127 L-CDR2 (SEQ ID 104) RTSRLAS
>81B4vK32 138 L-CDR2 (SEQ ID 104) RTSRLAS
>81B4vK32 140 L-CDR2 (SEQ ID 105) RTSQLAS
>81B4vK32 141 L-CDR2 (SEQ ID 106) RTSKLAS
>81B4vK32 147 L-CDR2 (SEQ ID 140) RTSH LAS
>73C5vK39_2 L-CDR2 SASYRHS (SEQ ID NO: 36) >73C5vK39_7 L-CDR2 SASYRHS (SEQ ID NO: 36) >73C5vK39_15 L-CDR2 SASYRHS (SEQ ID NO: 36) L-CDR3 Amino Acid Sequences >81B4vK32_3 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >81B4vK32_105 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >81B4vK32_116 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >81B4vK32_127 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >81B4vK32_138 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >81B4vK32_140 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >81B4vK32_141 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >81B4vK32_147 L-CDR3 HQFHRSPLT (SEQ ID NO: 44) >73C5vK39_2 L-CDR3 QQYSRYPLT (SEQ ID NO: 45) >73C5vK39_7 L-CDR3 QQYSRYPLT (SEQ ID NO: 45) >73C5vK39_15 L-CDR3 QQYSRYPLT (SEQ ID NO: 45) H-CDR1 Amino Acid Sequences >81B4vH33_49 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >81B4vH33_85T H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >81B4vH33_90 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >81B4vH33 93 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >81B4vH50_22 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >81B4vH50_30 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >81B4vH51_13 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >81B4vH51_15 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >81B4vH52_83 H-CDR1 GYSFTSSWIH (SEQ ID NO: 53) >73C5vH46_4 H-CDR1 GFSLTDYAVH (SEQ ID NO: 107) >73C5vH46_19 H-CDR1 GFSLTDYAVH (SEQ ID NO: 107) >73C5vH46_40 H-CDR1 GFSLTDYAVH (SEQ ID NO: 107) >73C5vH47_65 H-CDR1 GFSLTDYAVH (SEQ ID NO: 107) >73C5vH47_77 H-CDR1 GFSLTDYAVH (SEQ ID NO: 107) >73C5vH58_91 H-CDR1 GFSLTDYAVH (SEQ ID NO: 107) H-CDR2 Amino Acid Sequences >81B4vH33_49 H-CDR2 EINPGNVRTNYNENF (SEQ ID NO: 62) >81B4vH33_85T H-CDR2 EINPGNVRTNYNENF (SEQ ID NO: 62) >81B4vH33_90 H-CDR2 EINPGNVRTNYNENF (SEQ ID NO: 62) >81B4vH33_93 H-CDR2 EINPGNVRTNYNENF (SEQ ID NO: 62) >81B4vH50_22 H-CDR2 EILPGVVRTNYNENF (SEQ ID NO: 108) >81B4vH50_30 H-CDR2 EINPGAVRTNYNENF (SEQ ID NO: 109) >81B4vH51_13 H-CDR2 EINPGLVRTNYNENF (SEQ ID NO: 110) >81B4vH51_15 H-CDR2 EINPGAVRTNYNENF (SEQ ID NO: 109) >81B4vH52_83 H-CDR2 EINPGSVRTNYNENF (SEQ ID NO: 111) >73C5vH46_4 H-CDR2 VIWSDGSTDYNAPFKS (SEQ ID NO: 64) >73C5vH46_19 H-CDR2 VIWSDGSTDYNAPFKS (SEQ ID NO: 64) >73C5vH46_40 H-CDR2 VIWSDGSTDYNAPFKS (SEQ ID NO: 64) >73C5vH47_65 H-CDR2 VIWSDGSTDYNAPFKS (SEQ ID NO: 64) >73C5vH47_77 H-CDR2 VIWSDGSTDFNAPFKS (SEQ ID NO: 63) >73C5vH58_91 H-CDR2 VIWSDGSTDYNAPFKS (SEQ ID NO: 64) H-CDR3 Amino Acid Sequences >81B4vH33_49 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >81B4vH33_85T H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >81B4vH33_90 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >81B4vH33_93 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >81B4vH50_22 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >81B4vH50_30 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >81B4vH51_13 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >81B4vH51_15 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >81B4vH52_83 H-CDR3 VFYGEPYFPY (SEQ ID NO: 72) >73C5vH46_4 H-CDR3 KGGYSGSWFAY (SEQ ID NO: 73) >73C5vH46_19 H-CDR3 KGGYSGSWFAY (SEQ ID NO: 73) >73C5vH46_40 H-CDR3 KGGYSGSWFAY (SEQ ID NO: 73) >73C5vH47_65 H-CDR3 KGGYSGSWFAY (SEQ ID NO: 73) >73C5vH47_77 H-CDR3 KGGYSGSWFAY (SEQ ID NO: 73) >73C5vH58_91 H-CDR3 KGGYSGSWFAY (SEQ ID NO: 73)

[00080] In one aspect, a variable region of the present invention is linked to a constant region. For example, a variable region of the present invention is linked to a constant region shown below to form a heavy chain or a light chain of an antibody.

Heavy Chain Constant region linked downstream of a humanized variable heavy region:
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQ
SSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPE
AAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH NAKTK
PREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQV
YTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFL
YSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO: 112) Light Chain Constant region linked downstream of a humanized variable light region:
RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTE
QDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID
NO:113)

[00081] Representative light chain and heavy chain sequences of the present invention are shown below (humanized variable regions derived from antibodies 8164 and 73C5 linked to constant regions).
Light Chain Amino Acid Sequences >81B4yK32_3 Light Chain E IVLTQSPGTLSLSPGE RATMSCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSTLASG I
PDRFSGSGSGTDFTLTISRLEPEDAATYYCHQFHRSPLTFGQGTKLEIKRTVAAPSVFI
FPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYS
LSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 114) >81B4yK32_105 Light Chain E IVLTQSPGTLSLSPGE RATMSCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSILASGV
PDRFSGSGSGTDFTLTISRLEPEDFATYYCHQFHRSPLTFGQGTKLEIKRTVAAPSVFIF
PPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSL
SSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 115) >81B4yK32_116 Light Chain E IVLTQSPGTLSLSPGE RATMSCTASSSVSSSYFHWYQQKPGQAPRLWIYRTSRLASG
VPDRFSGSGSGTDFTLTISRLEPEDAATYYCHQFHRSPLTFGQGTKLEIKRTVAAPSVF
IFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTY
SLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 116) >81B4vK32 127 Light Chain E IVLTQSPGTLSLSPGE RATMTCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSRLASG
VPDRFSGSGSGTDFTLTISRLEPEDFAVYYCHQFHRSPLTFGQGTKLEIKRTVAAPSVF
IFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTY
SLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 117) >81B4vK32 138 Light Chain QIVLTQSPGTLSLSPGERATMTCTASSSVSSSYFHWYQQKPGQAPRLWIYRTSRLAS
GVPDRFSGSGSGTDFTLTISRLEPEDAATYYCHQFHRSPLTFGAGTKLEIKRTVAAPSV
FIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDST
YSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 118) >81B4vK32 140 Light Chain QIVLTQSPGTLSLSPGERVTMSCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSQLASG
IPDRFSGSGSGTDFTLTISRLEPEDAATYYCHQFHRSPLTFGQGTKLEIKRTVAAPSVFI
FPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYS
LSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 119) >81 B4vK32 141 Light Chain QIVLTQSPGTLSLSPGERATMTCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSKLASG
VPDRFSGSGSGTDFTLTISRLEPEDFATYYCHQFHRSPLTFGQGTKLEIKRTVAAPSVF
IFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTY
SLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 120) >81B4vK32 147 Light Chain E IVLTQSPGTLSLSPGE RATMSCTASSSVSSSYFHWYQQKPGQAPRLLIYRTSHLASG I
PG RFSGSGSGTDFTLTIS RLE P EDAAVYYCHQFH RS PLTFGQGTKLE I K RTVAAPSVFI
FPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYS
LSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 121) >73C5vK39 2 Light Chain E IVMTQSPATLSVSPGVRATLSCKASQDVGTNVLWYQQKPGQAP RPLIYSASYRHSG I
P DRFSGSGSGTEFTLT ISSLQSEDFAEYFCQQYSRY PLTFGQGTKLE I KRTVAA PSVFI
FPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYS
LSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 122) >73C5vK39 7 Light Chain E IVMTQSPATLSVSPGVRATLSCKASQDVGTNVLWYQQKPGQAP RPLIYSASYRHSG I
PDRFSGSGSGTEFTLTISSLOSEDFAVYYCQQYSRYPLTFGQGTKLEIKRTVAAPSVFI
FPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYS
LSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 123) >73C5vK39 15 Light Chain E IVMTQSPATLSVSPGVRATLSCKASQDVGTNVLWYQQKPGQAP RPLIYSASYRHSG I
PARFSGSGSGTEFTLTISSLQSE DFAEYYCQQYSRYPLTFGQGTKLE IKRTVAAPSVFI
FPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYS
LSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO: 124) Heavy Chain Amino Acid Sequences >81B4vH33 49 Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWI HWVRQAPGQG LE WIG El N PG NV
RTNYNENFRNKATMTVDTSISTAYMELSRLRSDDTAVYYCAVVFYGE PYFPYWGQGT
LVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHT
FPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCP
PCPAPEAAGGPSVFLFPPKPKDTLM IS RTPEVTCVVVDVSH ED PEVKFNWYVDGVEV
HNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ
PRE PQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLD
SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
125) >81B4vH33 85T Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWI HWVRQRPGQG LE WIG El N PG NV
RTNYNENFRNRVTMTVDTSISTAYMELSRLRSDDTAVYYCTVVFYGE PYFPYWGQGT
LVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHT
FPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCP
PCPAPEAAGGPSVFLFPPKPKDTLM IS RTPEVTCVVVDVSH ED PEVKFNWYVDGVEV
HNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ
PRE PQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLD
SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
126) >81B4vH33 90 Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWIHWVKQAPGQG LEWMGE IN PG NV
RTNYNENFRNKVTMTVDTSISTAYMELSRLRSDDTAVYYCTVVFYGEPYFPYWGQGT
LVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHT
FPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCP
PCPAPEAAGGPSVFLFPPKPKDTLM IS RTPEVTCVVVDVSH ED PEVKFNWYVDGVEV
HNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ
PRE PQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLD
SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
127) >81B4vH33 93 Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWIHWVRQRPGQGLEWMGE IN PGNV
RTNYNENFRN RATLTRDTSISTAYMELSRLRSDDTAVYYCAVVFYGEPYFPYWGQGTL
VTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF
PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP P
CPAPEAAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH
NAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP
REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
128) >81B4vH50 22 Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWIHWVRQRPGQGLEWMGEILPGVV
RTNYNENFRNKVTMTVDTSISTAYMELSRLRSDDTAVYYCTVVFYGEPYFPYWGQGT
LVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHT
FPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCP
PCPAPEAAGG PSVFLFP PKPKDTLM IS RTPEVTCVVVDVSH ED PEVKFNWYVDGVEV
HNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ
PRE PQVYTLP PSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTP PVLD
SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
129) >81B4vH50 30 Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWI HWVRQRPGQG LE WIG El N PGAV
RTNYNENFRNRVTMTVDTSISTAYMELSRLRSDDTAVYYCTVVFYGE PYFPYWGQGT
LVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHT
FPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCP
PCPAPEAAGG PSVFLFP PKPKDTLM IS RTPEVTCVVVDVSH ED PEVKFNWYVDGVEV
HNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQ
PRE PQVYTLP PSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTP PVLD
SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
130) >81B4vH51 13 Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWI HWVRQAPGQG LE WIG El N PG LVR
TNYN EN FRN KVTMTVDTS ISTAYME LS RLRSDDTAVYYCAVVFYG E PYFPYWGQGTL
VTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF
PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP P
CPAPEAAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH
NAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP
REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
131) >81B4vH51 15 Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWI HWVRQAPGQG LE WIG El NPGAVR
TNYN EN FRN KVTMTVDTS ISTAYME LS RLRSDDTAVYYCAVVFYG E PYFPYWGQGTL
VTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF
PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP P
CPAPEAAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH
NAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP
REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
132) >81B4vH52 83 Heavy Chain QVQLVQSGAEVKKPGASVKVSCKASGYSFTSSWI HWVRQAPGQG LE WIG El NPGSVR
TNYN EN FRN KATMTVDTS ISTAYME LS RLRSDDTAVYYCAVVFYG E PYFPYWGQGTL
VTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF
PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP P
CPAPEAAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH
NAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP
REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
133) >73C5vH46_4 Heavy Chain QVQLQESG PGLVKPSETLSITCTVSGFSLTDYAVHWI RQP PGKGLEWIGVIWSDGSTD
YNAPFKSRVTINKDTSKSQVSFKMSSVQAADTAVYYCARKGGYSGSWFAYWGQGTL
VTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF
PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP P
CPAPEAAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH
NAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP
REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
134) >73C5vH46 19 Heavy Chain QVQLQESG PGLVKPSETLSITCTVSGFSLTDYAVHWI RQP PGKGLEWIGVIWSDGSTD
YNAPFKSRVTISKDTSKNQVSLKMNSLTTDDTAVYYCARKGGYSGSWFAYWGQGTLV
TVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFP
AVLQSSG LYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP PC
PAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHN
AKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPR
EPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSD
GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
135) >73C5vH46_40 Heavy Chain QVQLQESG PG LVKPSETLSITCTVSGFSLTDYAVHWI RQP PGKG LEWIGVIWSDGSTD
YNAPFKSRVTISKDNSKSQVSLKMNSVTVADTAVYYCARKGGYSGSWFAYWGQGTL
VTVSSASTKG PSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF
PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP P
CPAPEAAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH
NAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP
REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
136) >73C5vH47 65 Heavy Chain QVQLQESG PG LVKPSETLSITCTVSGFSLTDYAVHWVRQPPGKGLEWIGVIWSDGST
DYNAPFKSRVTISKDTSKNQVSFKLSSVTVDDTAVYYCARKGGYSGSWFAYWGQGTL
VTVSSASTKG PSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF
PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP P
CPAPEAAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH
NAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP
REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
137) >73C5vH47 77 Heavy Chain QVQLQESG PG LVAPSETLSLTCTVSGFSLTDYAVHWI RQFPGKGLEWIGVIWSDGSTD
FNAPFKSRVTISKDTSKNQVSFKLSSVTTDDTAVYYCARKGGYSGSWFAYWGQGTLV
TVSSASTKG PSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFP
AVLQSSG LYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP PC
PAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHN
AKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPR
EPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSD
GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
138) >73C5vH58 91 Heavy Chain QVQLQESG PG LVKPSETLSITCTVSGFSLTDYAVHWI RQP PGKG LEWIGVIWSDGSTD
YNAPFKSRVTISKDNSKSQVSFKMSSVTADDTAVYYCARKGGYSGSWFAYWGQGTL
VTVSSASTKG PSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF
PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN H KPSNTKVDKRVE PKSCDKTHTCP P
CPAPEAAGG PSVFLFPPKPKDTLM ISRTPEVTCVVVDVSH ED PEVKFNWYVDGVEVH
NAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP
REPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO:
139)

[00082] The CDRs listed above are defined using the Chothia numbering system (AI-Lazikani et al., (1997) JMB 273, 927-948).

[00083] In one aspect, an antibody of the present invention comprises 3 light chain CDRs and 3 heavy chain CDRs, for example as set forth above.

[00084] In one aspect, an antibody of the present invention comprises a light chain and a heavy chain variable region as set forth above. In one aspect, a light chain variable region of the invention is fused to a light chain constant region, for example a kappa or lambda constant region. In one aspect, a heavy chain variable region of the invention is fused to a heavy chain constant region, for example IgA, IgD, IgE, IgG
or IgM, in particular, IgGi, IgG2, IgG3 or !gat.

[00085] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 125 (Antibody B1).

[00086] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 126 (Antibody B2).

[00087] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 127 (Antibody B3).

[00088] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 125 (Antibody B4).

[00089] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 126 (Antibody B5).

[00090] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 127 Antibody B6).

[00091] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 123; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 138 (Antibody C3).

[00092] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 123; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 139 (Antibody C2).

[00093] The present invention provides an anti-IL-36R antibody comprising a light chain comprising the amino acid sequence of SEQ ID NO: 124; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 138 (Antibody Cl)

[00094] Representative antibodies of the present invention are shown below.
Table B.
Anti Light Chain Sequences Heavy Chain Sequences body ASSSVSSSYFHWYQQKPGQAPR SFTSSWIHWVRQAPGQGLEWIGEINPGN
LLIYRTSILASGVPDRFSGSGSGT VRTNYNENFRNKATMTVDTSISTAYMEL
DFTLTISRLEPEDFATYYCHQFHR SRLRSDDTAVYYCAVVFYGEPYFPYWG
SPLTFGQGTKLEIKRTVAAPSVFIF QGTLVTVSSASTKGPSVFPLAPSSKSTS
PPSDEQLKSGTASVVCLLNNFYP GGTAALGCLVKDYFPEPVTVSWNSGALT
REAKVQWKVDNALQSGNSQESV SGVHTFPAVLQSSGLYSLSSVVTVPSSS
TEQDSKDSTYSLSSTLTLSKADYE LGTQTYICNVNHKPSNTKVDKRVEPKSC
KHKVYACEVTHQGLSSPVTKSFN DKTHTCPPCPAPEAAGGPSVFLFPPKPK
RGEC (SEQ ID NO: 115) DTLMISRTPEVTCVVVDVSHEDPEVKFN
WYVDGVEVHNAKTKPREEQYNSTYRVV
SVLTVLHQDWLNGKEYKCKVSNKALPAP
IEKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 125) ASSSVSSSYFHWYQQKPGQAPR SFTSSWIHWVRQRPGQGLEWIGEINPG
LLIYRTSILASGVPDRFSGSGSGT NVRTNYNENFRNRVTMTVDTSISTAYME
DFTLTISRLEPEDFATYYCHQFHR LSRLRSDDTAVYYCTVVFYGEPYFPYWG
SPLTFGQGTKLEIKRTVAAPSVFIF QGTLVTVSSASTKGPSVFPLAPSSKSTS
PPSDEQLKSGTASVVCLLNNFYP GGTAALGCLVKDYFPEPVTVSWNSGALT
REAKVQWKVDNALQSGNSQESV SGVHTFPAVLQSSGLYSLSSVVTVPSSS
TEQDSKDSTYSLSSTLTLSKADYE LGTQTYICNVNHKPSNTKVDKRVEPKSC
KHKVYACEVTHQGLSSPVTKSFN DKTHTCPPCPAPEAAGGPSVFLFPPKPK
RGEC (SEQ ID NO: 115) DTLMISRTPEVTCVVVDVSHEDPEVKFN

WYVDGVEVHNAKTKPREEQYNSTYRVV
SVLTVLHQDWLNGKEYKCKVSNKALPAP
IEKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 126) ASSSVSSSYFHWYQQKPGQAPR SFTSSWI HWVKQA PGQG LEWMG E I N PG
LLIYRTSILASGVPDRFSGSGSGT NVRTNYNENFRNKVTMTVDTSISTAYME
DFTLTISRLE PE DFATYYCHQFH R LSRLRSDDTAVYYCTVVFYGEPYFPYWG
SPLTFGQGTKLE I KRTVAA PSVFI F QGTLVTVSSASTKG PSVFPLAPSSKSTS
PPSDEQLKSGTASVVCLLNNFYP GGTAALGCLVKDYFPE PVTVSWNSGALT
REAKVQWKVDNALQSGNSQESV SGVHTFPAVLQSSGLYSLSSVVTVPSSS
TEQDSKDSTYSLSSTLTLSKADYE LGTQTYICNVNHKPSNTKVDKRVE PKSC
KHKVYACEVTHQGLSSPVTKSFN DKTHTCP PCPAPEAAGG PSVFLFP PKPK
RGEC (SEQ ID NO: 115) DTLMISRTPEVTCVVVDVSHEDPEVKFN
WYVDGVEVHNAKTKPREEQYNSTYRVV
SVLTVLHQDWLNGKEYKCKVSNKALPAP
IEKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 127) ASSSVSSSYFHWYQQKPGQAPR SFTSSWI HWVRQAPGQG LEW IGE IN PG N
LWIYRTSRLASGVPDRFSGSGSG VRTNYNENFRNKATMTVDTSISTAYMEL
TDFTLTISRLEPEDAATYYCHQFH SRLRSDDTAVYYCAVVFYGEPYFPYWG
RS PLTFGAGTKLE I K RTVAA PSVFI QGTLVTVSSASTKG PSVFPLAPSSKSTS
FP PSDEQLKSGTASVVCLLN N FYP GGTAALGCLVKDYFPE PVTVSWNSGALT
REAKVQWKVDNALQSGNSQESV SGVHTFPAVLQSSGLYSLSSVVTVPSSS
TEQDSKDSTYSLSSTLTLSKADYE LGTQTYICNVNHKPSNTKVDKRVE PKSC
KHKVYACEVTHQGLSSPVTKSFN DKTHTCP PCPAPEAAGG PSVFLFP PKPK
RGEC (SEQ ID NO: 118) DTLMISRTPEVTCVVVDVSHEDPEVKFN
WYVDGVEVHNAKTKPREEQYNSTYRVV
SVLTVLHQDWLNGKEYKCKVSNKALPAP
IEKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 125) ASSSVSSSYFHWYQQKPGQAPR SFTSSWI HWVRQ RPGQG LEW IGE IN PG
LWIYRTSRLASGVPDRFSGSGSG NVRTNYNENFRN RVTMTVDTSISTAYME
TDFTLTISRLEPEDAATYYCHQFH LSRLRSDDTAVYYCTVVFYGEPYFPYWG
RS PLTFGAGTKLE I K RTVAA PSVFI QGTLVTVSSASTKG PSVFPLAPSSKSTS
FP PSDEQLKSGTASVVCLLN N FY P GGTAALGCLVKDYFPE PVTVSWNSGALT
REAKVQWKVDNALQSGNSQESV SGVHTFPAVLQSSGLYSLSSVVTVPSSS
TEQDSKDSTYSLSSTLTLSKADYE LGTQTYICNVNHKPSNTKVDKRVE PKSC
KHKVYACEVTHQGLSSPVTKSFN DKTHTCP PCPAPEAAGG PSVFLFP PKPK
RGEC (SEQ ID NO: 118) DTLMISRTPEVTCVVVDVSHEDPEVKFN
WYVDGVEVHNAKTKPREEQYNSTYRVV
SVLTVLHQDWLNGKEYKCKVSNKALPAP
IEKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 126) QIVLTQSPGTLSLSPGE RATMTCT QVQLVQSGAEVKKPGASVKVSCKASGY
ASSSVSSSYFHWYQQKPGQAPR SFTSSWI HWVKQA PGQG LEWMG E I N PG
LWIYRTSRLASGVPDRFSGSGSG NVRTNYNENFRNKVTMTVDTSISTAYME
TDFTLTISRLEPEDAATYYCHQFH LSRLRSDDTAVYYCTVVFYGEPYFPYWG
RS PLTFGAGTKLE I K RTVAA PSVFI QGTLVTVSSASTKG PSVFPLAPSSKSTS
FP PSDEQLKSGTASVVCLLN N FY P GGTAALGCLVKDYFPE PVTVSWNSGALT
REAKVQWKVDNALQSGNSQESV SGVHTFPAVLQSSGLYSLSSVVTVPSSS
TEQDSKDSTYSLSSTLTLSKADYE LGTQTYICNVNHKPSNTKVDKRVE PKSC
KHKVYACEVTHQGLSSPVTKSFN DKTHTCP PCPAPEAAGG PSVFLFP PKPK
RGEC (SEQ ID NO: 118) DTLMISRTPEVTCVVVDVSHEDPEVKFN
WYVDGVEVHNAKTKPREEQYNSTYRVV
SVLTVLHQDWLNGKEYKCKVSNKALPAP
IEKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 127) Table C
An Light Chain Sequences Heavy Chain Sequences body Cl EIVMTQSPATLSVSPGVRATLSCK QVQLQESG PG LVAPS ETLSLTCTVSG FS
ASQDVGTNVLWYQQKPGQAP RP LTDYAVHWI RQFPG KG LEWIGVIWSDGS
LIYSASYRHSG I PARFSGSGSGTE TDFNAPFKSRVTISKDTSKNQVSFKLSSV
FTLTISSLQSEDFAEYYCQQYSRY TTDDTAVYYCARKGGYSGSWFAYWGQ
PLTFGQGTKLE IK RTVAAPSVFI FP GTLVTVSSASTKG PSVFPLAPSSKSTSG
PSDEQLKSGTASVVCLLNNFYPR GTAALGCLVKDYFPEPVTVSWNSGALTS
EAKVQWKVDNALQSGNSQESVT GVHTFPAVLQSSGLYSLSSVVTVPSSSL
EQDSKDSTYSLSSTLTLSKADYEK GTQTYICNVNHKPSNTKVDKRVE PKSCD
HKVYACEVTHQGLSSPVTKSFN R KTHTCP PCPAPEAAGG PSVFLFP PK PKD
GEC (SEQ ID NO: 124) TLMISRTPEVTCVVVDVSHEDPEVKFNW
YVDGVEVHNAKTKPREEQYNSTYRVVS
VLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 138) ASQDVGTNVLWYQQKPGQAP RP TDYAVHWI RQ P PG KG LEWIGVIWSDGST
LIYSASYRHSG I P D RFSGSGSGTE DYNAPFKSRVTISKDNSKSQVSFKMSSV
FTLTISSLQSEDFAVYYCQQYSRY TAD DTAVYYCA RKGGYSGSWFAYWGQ
PLTFGQGTKLE IK RTVAAPSVFI FP GTLVTVSSASTKG PSVFPLAPSSKSTSG
PSDEQLKSGTASVVCLLNNFYPR GTAALGCLVKDYFPEPVTVSWNSGALTS
EAKVQWKVDNALQSGNSQESVT GVHTFPAVLQSSGLYSLSSVVTVPSSSL
EQDSKDSTYSLSSTLTLSKADYEK GTQTYICNVNHKPSNTKVDKRVE PKSCD
HKVYACEVTHQGLSSPVTKSFN R KTHTCP PCPAPEAAGG PSVFLFP PK PKD
GEC (SEQ ID NO: 123) TLMISRTPEVTCVVVDVSHEDPEVKFNW
YVDGVEVHNAKTKPREEQYNSTYRVVS
VLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 139) ASQDVGTNVLWYQQKPGQAP RP LTDYAVHWI RQFPG KG LEWIGVIWSDGS
LIYSASYRHSG I P D RFSGSGSGTE TDFNAPFKSRVTISKDTSKNQVSFKLSSV
FTLTISSLQSEDFAVYYCQQYSRY TTDDTAVYYCARKGGYSGSWFAYWGQ
PLTFGQGTKLE IK RTVAAPSVFI FP GTLVTVSSASTKG PSVFPLAPSSKSTSG
PSDEQLKSGTASVVCLLNNFYPR GTAALGCLVKDYFPEPVTVSWNSGALTS
EAKVQWKVDNALQSGNSQESVT GVHTFPAVLQSSGLYSLSSVVTVPSSSL
EQDSKDSTYSLSSTLTLSKADYEK GTQTYICNVNHKPSNTKVDKRVE PKSCD
KTHTCP PCPAPEAAGG PSVFLFP PK PKD

HKVYACEVTHQGLSSPVTKSFN R TLM ISRTPEVTCVVVDVSHEDPEVKFNW
GEC (SEQ ID NO: 123) YVDGVEVHNAKTKPREEQYNSTYRVVS
VLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSREEMTK
NQVSLTCLVKGFYPSDIAVEWESNGQPE
NNYKTTPPVLDSDGSFFLYSKLTVDKSR
WQQGNVFSCSVMHEALHNHYTQKSLSL
SPGK (SEQ ID NO: 138)

[00095]
In some aspects, the humanized antibody displays blocking activity, whereby it decreases the binding of IL-36 ligand to IL-36 receptor by at least 45%, by at least 50%, by at least 55%, by at least 60%, by at least 65%, by at least 70%, by at least 75%, by at least 80%, by at least 85%, by at least 90%, or by at least 95%. The ability of an antibody to block binding of IL-36 ligand to the IL-36 receptor can be measured using competitive binding assays known in the art. Alternatively, the blocking activity of an antibody can be measured by assessing the biological effects of IL-36, such as the production of IL-8, IL-6, and GM-CSF to determine if signaling mediated by the IL-36 receptor is inhibited.

[00096]
In a further aspect, the present invention provides a humanized anti-IL-36R
antibody having favorable biophysical properties. In one aspect, a humanized anti-IL-36R antibody of the present invention is present in at least 90% monomer form, or in at least 92% monomer form, or in at least 95% monomer form in a buffer. In a further aspect, a humanized anti-IL-36R antibody of the present invention remains in at least 90% monomer form, or in at least 92% monomer form, or in at least 95% monomer form in a buffer for one month or for four months.

[00097]
In one aspect, a humanized antibody of the present invention is Antibody B1, Antibody B2, Antibody B3, Antibody B4, Antibody B5, Antibody B6, Antibody Cl, Antibody C2, or Antibody C3. Accordingly, in one embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID NO:115 and the heavy chain sequence of SEQ ID NO:125 (Antibody B1). In another embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID NO:115 and the heavy chain sequence of SEQ ID NO:126 (Antibody B2). In another embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID NO:115 and the heavy chain sequence of SEQ ID
NO:127 (Antibody B3). In another embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID NO:118 and the heavy chain sequence of SEQ ID NO:125 (Antibody B4). In another embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID

NO:118 and the heavy chain sequence of SEQ ID NO:126 (Antibody B5). In another embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID NO:118 and the heavy chain sequence of SEQ ID NO:127 (Antibody B6). In another embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID NO:124 and the heavy chain sequence of SEQ ID NO:138 (Antibody Cl). In another embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID NO:123 and the heavy chain sequence of SEQ ID NO:139 (Antibody C2). In another embodiment, a humanized antibody of the present invention comprises the light chain sequence of SEQ ID NO:123 and the heavy chain sequence of SEQ ID NO:138 (Antibody C3).

[00098] In a further embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ ID NO:115 and the heavy chain sequence of SEQ ID NO:125 (Antibody B1). In another embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ ID NO:115 and the heavy chain sequence of SEQ ID NO:126 (Antibody B2). In another embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ ID NO:115 and the heavy chain sequence of SEQ ID NO:127 (Antibody B3). In another embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ ID NO:118 and the heavy chain sequence of SEQ ID
NO:125 (Antibody B4). In another embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ ID NO:118 and the heavy chain sequence of SEQ ID NO:126 (Antibody B5). In another embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ
ID
NO:118 and the heavy chain sequence of SEQ ID NO:127 (Antibody B6). In another embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ ID NO:124 and the heavy chain sequence of SEQ ID NO:138 (Antibody Cl). In another embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ ID NO:123 and the heavy chain sequence of SEQ ID NO:139 (Antibody C2). In another embodiment, a humanized antibody of the present invention consists of the light chain sequence of SEQ ID NO:123 and the heavy chain sequence of SEQ ID NO:138 (Antibody C3).

[00099] In some embodiments, the humanized anti-IL-36R antibodies, including antigen-binding fragments thereof, such as heavy and light chain variable regions, comprise an amino acid sequence of the residues derived from Antibody B1, Antibody B2, Antibody B3, Antibody B4, Antibody B5, Antibody B6, Antibody Cl, Antibody C2, or Antibody C3.

[000100] In a further embodiment, the present invention provides an anti-IL-antibody or antigen-binding fragment thereof that competitively binds to human anti-IL-36R with an antibody of the present invention, for example Antibody B1, Antibody B2, Antibody B3, Antibody B4, Antibody B5, Antibody B6, Antibody Cl, Antibody or Antibody C3 described herein. The ability of an antibody or antigen-binding fragment to competitively bind to IL-36R can be measured using competitive binding assays known in the art.

[000101] The humanized anti-IL-36R antibodies optionally include specific amino acid substitutions in the consensus or germline framework regions. The specific substitution of amino acid residues in these framework positions can improve various aspects of antibody performance including binding affinity and/or stability, over that demonstrated in humanized antibodies formed by "direct swap" of CDRs or HVLs into the human germline framework regions.

[000102] In some embodiments, the present invention describes other monoclonal antibodies with a light chain variable region having the amino acid sequence set forth in any one of SEQ ID NO:1-10. In some embodiments, the present invention describes other monoclonal antibodies with a heavy chain variable region having the amino acid sequence set forth in any one of SEQ ID NO:11-20. Placing such CDRs into FRs of the human consensus heavy and light chain variable domains will yield useful humanized antibodies of the present invention.

[000103] In particular, the present invention provides monoclonal antibodies with the combinations of light chain variable and heavy chain variable regions of SEQ
ID
NO:1/11, 2/12, 3/13, 4/14, 5/15, 6/16, 7/17, 8/18, 9/19, 10/20. Such variable regions can be combined with human constant regions.

[000104] In some embodiments, the present invention describes other humanized antibodies with light chain variable region sequences having the amino acid sequence set forth in any one of SEQ ID NO:76-86. In some embodiments, the present invention describes other humanized antibodies with heavy chain variable region sequences having the amino acid sequence set forth in any one of SEQ ID NO:87-101. In particular, the present invention provides monoclonal antibodies with the combinations of light chain variable and heavy chain variable regions of SEQ
ID NO:
77/89, 80/88, 80/89, 77/87, 77/88, 80/87, 86/100, 85/101, 85/100. Such variable regions can be combined with human constant regions.

[000105] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:77 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:77 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:89 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:89. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000106] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:80 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:80 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:88 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:88. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000107] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:80 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:80 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:89 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:89. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000108] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:77 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:77 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:87 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:87. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000109] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:77 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:77 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:88 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:88. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000110] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:80 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:80 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:87 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:87. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000111] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:86 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:86 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:100 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:100. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000112] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:85 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:85 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:101 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:101. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000113] In a further embodiment, the present invention relates to an anti-antibody or antigen-binding fragment thereof comprising a humanized light chain variable domain comprising the CDRs of SEQ ID NO:85 and framework regions having an amino acid sequence at least 90% identical, at least 93% identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain light chain amino acid sequence of SEQ ID NO:85 and a humanized heavy chain variable domain comprising the CDRs of SEQ ID NO:100 and framework regions having an amino acid sequence at least 90% identical, at least 93%
identical or at least 95% identical to the amino acid sequence of the framework regions of the variable domain heavy chain amino acid sequence of SEQ ID NO:100. In one embodiment, the anti-IL-36R antibody is a humanized monoclonal antibody.

[000114] In some specific embodiments, the humanized anti-IL-36R antibodies disclosed herein comprise at least a heavy or a light chain variable domain comprising the CDRs or HVLs of the murine monoclonal antibodies or humanized antibodies as disclosed herein and the FRs of the human germline heavy and light chain variable domains.

[000115] In one further aspect, the present invention provides an anti-IL-antibody or antigen-binding fragment thereof comprising a light chain CDR1 (L-CDR1) sequence of any one of SEQ ID NO:21-29; a light chain CDR2 (L-CDR2) sequence of any one of SEQ ID NO:30-38; a light chain CDR3 (L-CDR3) sequence of any one of SEQ ID NO:39-47; a heavy chain CDR1 (H-CDR1) sequence of any one of SEQ ID
NO:48-56; a heavy chain CDR2 (H-CDR2) sequence of any one of SEQ ID NO:57-66; and a heavy chain CDR3 (H-CDR3) sequence of any one of SEQ ID NO:67-75. In one aspect, the anti-IL-36R antibody or antigen-binding fragment thereof comprises a light chain variable region comprising a L-CDR1 listed above, a L-CDR2 listed above and a L-CDR3 listed above, and a heavy chain variable region comprising a H-listed above, a H-CDR2 listed above and a H-CDR3 listed above.

[000116] In a further aspect, the present invention provides an anti-IL-36R
antibody or antigen-binding fragment thereof comprising:
a) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:21, 30, 39, 48, 57 and 67, respectively; or b) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:22, 31, 40, 49, 58 and 68, respectively; or c) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:23, 32, 41, 50, 59 and 69, respectively; or d) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:24, 33, 42, 51, 60 and 70, respectively; or e) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:25, 34, 43, 52, 61 and 71, respectively; or f) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:26, 35, 44, 53, 62 and 72, respectively; or g) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:27, 36, 45, 54, 63 and 73, respectively; or h) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:27, 36, 45, 54, 64 and 74, respectively; or i) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:27, 36, 45, 54, 64 and 73, respectively; or j) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:28, 37, 46, 55, 65 and 74, respectively; or k) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:29, 38, 47, 56, 66 and 75, respectively.
In a further aspect, the present invention provides an anti-IL-36R antibody or antigen-binding fragment thereof comprising:
a) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:26, 103, 44, 53, 62 and 72, respectively; or b) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:26, 104, 44, 53, 62 and 72, respectively; or c) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:27, 36, 45, 107, 63 and 73, respectively; or d) a L-CDR1, a L-CDR2, a L-CDR3, a H-CDR1, a H-CDR2 and a H-CDR3 sequence of SEQ ID NO:27, 36, 45, 107, 64 or 73, respectively.

[000117] In one aspect, the anti-IL-36R antibody or antigen-binding fragment thereof comprises a light chain variable region comprising a L-CDR1, L-CDR2 and L-CDR3 combination listed above, and a heavy chain variable region comprising a H-CDR1, H-CDR2 and H-CDR3 combination listed above.

[000118] In specific embodiments, it is contemplated that chimeric antibodies with switched CDR regions (i.e., for example switching one or two CDRs of one of the mouse antibodies or humanized antibody derived therefrom with the analogous CDR
from another mouse antibody or humanized antibody derived therefrom) between these exemplary immunoglobulins may yield useful antibodies.

[000119] In certain embodiments, the humanized anti-IL-36R antibody is an antibody fragment. Various antibody fragments have been generally discussed above and there are techniques that have been developed for the production of antibody fragments.
Fragments can be derived via proteolytic digestion of intact antibodies (see, e.g., Morimoto et al., 1992, Journal of Biochemical and Biophysical Methods 24:107-117;
and Brennan et al., 1985, Science 229:81). Alternatively, the fragments can be produced directly in recombinant host cells. For example, Fab'-SH fragments can be directly recovered from E. coli and chemically coupled to form F(ab')2 fragments (see, e.g., Carter et al., 1992, Bio/Technology 10:163-167). By another approach, F(ab')2 fragments can be isolated directly from recombinant host cell culture. Other techniques for the production of antibody fragments will be apparent to the skilled practitioner. Accordingly, in one aspect, the present invention provides antibody fragments comprising the CDRs described herein, in particular one of the combinations of L-CDR1, L-CDR2, L-CDR3, H-CDR1, H-CDR2 and H-CDR3 described herein. In a further aspect, the present invention provides antibody fragments comprising the variable regions described herein, for example one of the combinations of light chain variable regions and heavy chain variable regions described herein.

[000120] Certain embodiments include an F(ab')2 fragment of a humanized anti-IL-36R antibody comprise a light chain sequence of any of SEQ ID NO: 115 or 118 in combination with a heavy chain sequence of SEQ ID NO: 125, 126 or 127. Such embodiments can include an intact antibody comprising such an F(ab')2.

[000121] Certain embodiments include an F(ab')2 fragment of a humanized anti-IL-36R antibody comprise a light chain sequence of any of SEQ ID NO: 123 or 124 in combination with a heavy chain sequence of SEQ ID NO: 138 or 139. Such embodiments can include an intact antibody comprising such an F(ab')2.

[000122] In some embodiments, the antibody or antibody fragment includes a constant region that mediates effector function. The constant region can provide antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP) and/or complement-dependent cytotoxicity (CDC) responses against an anti-IL-36R expressing target cell. The effector domain(s) can be, for example, an Fc region of an Ig molecule.

[000123] The effector domain of an antibody can be from any suitable vertebrate animal species and isotypes. The isotypes from different animal species differ in the abilities to mediate effector functions. For example, the ability of human immunoglobulin to mediate CDC and ADCC/ADCP is generally in the order of IgIVP---IgG1::---IgG3>IgG2>IgG4 and IgG1,,--IgG3>IgG2/IgM/IgG4, respectively.
Murine immunoglobulins mediate CDC and ADCC/ADCP generally in the order of murine IgM,---IgG3 IgG2b>IgG2a IgG1 and IgG2b>IgG2a>lgG1 IgG3, respectively. In another example, murine IgG2a mediates ADCC while both murine IgG2a and IgM
mediate CDC.
III. Pharmaceutical Doses and Administration

[000124] Anti-IL-36R antibodies of the present invention are typically administered to a patient as a pharmaceutical composition in which the antagonist is admixed with a pharmaceutically acceptable carrier or excipient, see, e. g., Remington's Pharmaceutical Sciences and US. Pharmacopeia: National Formulary, Mack Publishing Company, Easton, Pa. (1984). The pharmaceutical composition may be formulated in any manner suitable for the intended route of administration.
Examples of pharmaceutical formulations include lyophilized powders, slurries, aqueous solutions, suspensions and sustained release formulations (see, e. g. , Hardman et al.
(2001) Goodman and Gilman's The Pharmacological Basis of Therapeutics, McGraw-Hill, New York, N. Y. ; Gennaro (2000) Remington: The Science and Practice of Pharmacy, Lippincott, Williams, and Wilkins, New York, N. Y.; Avis et al.(eds.) (1993) Pharmaceutical Dosage Forms: Parenteral Medications, Marcel Dekker, NY;
Lieberman et al. (eds.) (1990) Pharmaceutical Dosage Forms: Tablets, Marcel Dekker, NY; Lieberman et al. (eds. ) (1990) Pharmaceutical Dosage Forms:
Disperse Systems, Marcel Dekker, NY; Weiner and Kotkoskie (2000) Excipient Toxicity and Safety, Marcel Dekker, Inc. , New York, N. Y. ). Suitable routes of administration include intravenous injection (including intraarterial injection) and subcutaneous injection.

[000125] Representative examples of doses and dose regimens according to the present invention are disclosed in Table A. Although, doses 900 mg and 750 mg have been exemplified, similar dose regimens equally apply to doses 210 mg, 300 mg, mg, 450 mg, 600 mg, 700 mg and 800 mg.
Table A: Doses and Dose Regimens C 0 .- 0 .- 0 c 0 00 .-o o o o o 0 -0 o o .->. - >, o o ... 0 .... 0 o>.= o -0 ,- up ..., > o E z > o > - =__- -_. o E
z mt c Ci -' '0 =-= z z µ... Z Z c LL = u_ = - 1:4 z u) u_ g z -0 900 2 Second dose 1 week - - -after the first dose 900 2 Second dose 1 week 150 1 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 150 2 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 150 3 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week - - -after the first dose 900 2 Second dose 1 week 225 1 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 225 2 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 225 3 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 300 1 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 300 2 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 300 3 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 600 1 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 600 2 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 2 Second dose 1 week 600 3 or more At 2, 4, 6, 8, 10 or after the first dose 12 weeks intervals 900 3 Second dose 1 week - - -after the first dose;
third dose 2, 4, 6, 8, or 12 weeks after the second dose 900 3 Second dose 1 week 150 1 or more At 2, 4, 6, 8, 10 or after the first dose;
12 weeks third dose 1, 2, 4, 6, 8, 10 or 12 weeks intervals after the second dose 900 3 Second dose 1 week 150 2 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals 10 or 12 weeks after the second dose 900 3 Second dose 1 week 150 3 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals 10 or 12 weeks after the second dose 900 3 Second dose 1 week 225 1 or more At 2, 4, 6, 8, 10 or after the first dose;
12 weeks third dose 2, 4, 6, 8, 10 or 12 weeks after intervals the second dose 900 3 Second dose 1 week 225 2 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals 10 or 12 weeks after the second dose 900 3 Second dose 1 week 225 3 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals 10 or 12 weeks after the second dose 900 3 Second dose 1 week 300 1 or more At 2, 4, 6, 8, 10 or after the first dose;
12 weeks third dose 2, 4, 6, 8, 10 or 12 weeks after intervals the second dose 900 3 Second dose 1 week 300 2 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals 10 or 12 weeks after the second dose 900 3 Second dose 1 week 300 3 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals 10 or 12 weeks after the second dose 900 3 Second dose 1 week 600 1 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals or 12 weeks after the second dose 900 3 Second dose 1 week 600 2 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals 10 or 12 weeks after the second dose 900 3 Second dose 1 week 600 3 or more At 2, 4, 6, 8, 10 or after the first dose; 12 weeks third dose 2, 4, 6, 8, intervals 10 or 12 weeks after the second dose

[000126] In one embodiment, 1, 2 or 3 or more intravenous dose(s) is/are administered to the patient in a dose regimen listed in Table Aõ wherein a first subcutaneous dose is administered 2 to 8 weeks, 4 to 6 weeks, 2 weeks, 4 weeks, 6 weeks or 8 weeks, 12 weeks, 16 weeks, 20 weeks after the last intravenous dose, and subsequent subcutaneous doses are administered at 2, 4, 6, 8, 10 or 12 weeks intervals after the first subcutaneous dose.

[000127] In one aspect, the invention relates to the treatment of GPP in a patient by administering to the patient two 900 mg intravenous (i.v.) doses of an anti-IL-antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[000128] In another aspect, the invention relates to the treatment of GPP
in a patient by administering to the patient a single 900 mg intravenous dose of an anti-IL-antibody, if the GPP symptoms persist, administering an additional 900 mg intravenous dose an anti-IL-36R antibody one week after the initial dose.

[000129] In one aspect, the invention relates to a method of treating generalized pustular psoriasis (GPP) flares in a patient, said method comprising administering to the patient two 900 mg intravenous (i.v.) doses of an anti-IL-36R antibody;
wherein the second dose is administered less than 2 weeks after the first dose.

[000130] In one aspect, the invention relates to a method of treating GPP
in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[000131] In one aspect, the invention relates to a method of reducing or alleviating signs and symptoms of an acute phase flare-up of GPP in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[000132] In one aspect, the invention relates to a method of reducing the severity and duration of GPP symptoms, said method comprising including administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[000133] In one aspect, the invention relates to a method of treating a skin disorder associated with GPP, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[000134] In one aspect, the invention relates to a method of preventing the recurrence of GPP flares in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[000135] In one aspect, the invention relates to a method of reducing pain by at least 10% in a patient with GPP, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[000136] In one aspect, the invention relates to a method of improving the quality of life by at least 10% in a patient with moderate to severe GPP symptoms, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

[000137]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of 2.

[000138]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2.

[000139]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before the administration of the first i.v. dose.

[000140]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 after the administration of the first i.v. dose.

[000141]
In an embodiment relating to any of the above aspects, the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before and after the administration of the first i.v. dose.

[000142]
In an embodiment relating to any of the above aspects, the second dose is administered after 1 week but less than 2 weeks from the first dose.

[000143]
In one aspect, the invention relates to a method of treating a GPP patient with a GPPGA pustulation subscore of 2, said method comprising the steps of:
(a) administering to the patient a first 900 mg intravenous (I.V.) dose of an anti-antibody; (b) assessing the GPPGA pustulation subscore of the patient and if the GPPGA pustulation subscore of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

[000144]
In one aspect, the invention relates to a method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of 2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (i.v.) dose of an anti-IL-36R antibody; (b) assessing the GPPGA total score of the patient and if the GPPGA total score of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

[000145]
In one aspect, the invention relates to a method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (i.v.) dose of an anti-IL-36R antibody;
(b) assessing the GPPGA scores of the patient and if the GPPGA total score of 2 and the GPPGA pustulation subscore of 2 of the patient persist after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

[000146]
In an embodiment relating to any of the above aspects or embodiments, an optional third 900 mg i.v. dose of the anti-IL-36R antibody is administered at 2 to 12 weeks after the second i.v. dose.

[000147]
In an embodiment relating to any of the above aspects or embodiments, the two-dose administration achieves one or more of the following results:
(a) a Generalized Pustular Psoriasis Global Assessment (GPPGA) pustulation subscore of 0 indicating in one week after administering the second i.v. dose;
and/or (b) a GPPGA total score of 0 or 1 in one week after administering the second i.v. dose.

[000148]
In an embodiment relating to any of the above aspects or embodiments, the results are maintained for up to and at least 12 weeks following the administration of the second i.v. dose.

[000149]
In an embodiment relating to any of the above aspects or embodiments, the method comprises administering to the patient a prophylactically effective amount of the anti-IL-36R antibody in one or more subcutaneous doses after the last i.v.
dose administered.

[000150] In an embodiment relating to any of the above aspects or embodiments, each of the one or more subcutaneous doses comprises 150 mg, 225 mg, 300 mg, 450 mg, or 600 mg of said anti-IL-36R antibody.

[000151] In an embodiment relating to any of the above aspects or embodiments, 1, 2, 3 or more subcutaneous doses are administered to the patient and wherein a first subcutaneous dose is administered after the last intravenous dose.

[000152] In an embodiment relating to any of the above aspects or embodiments, a first subcutaneous dose is administered 2 to 8 weeks, 4 to 6 weeks, 2 weeks, 4 weeks, 6 weeks or 8 weeks, 12 weeks, 16 weeks, 20 weeks after the last intravenous dose, and subsequent subcutaneous doses are administered at 2, 4, 6, 8, 10 or 12 weeks intervals after the first subcutaneous dose.

[000153] In an embodiment relating to any of the above aspects or embodiments, the patient remains in clinical remission as measured by a GPPGA total score of 0 or 1 for at least 12, 24, 36, 48, 60 or 72 weeks following the last i.v. or subcutaneous dose.

[000154] In an embodiment relating to any of the above aspects or embodiments, the mammal or the patient is evaluated for improved Clinical Remission as defined by: (a) Generalized Pustular Psoriasis Global Assessment (GPPGA) score of 0 or 1 at Week 1; (b) GPPGA pustulation subscore of 0 indicating no visible pustules at Week 1; (c) Psoriasis Area and Severity Index for Generalized Pustular Psoriasis (GPPASI) 75 at Week 4; (d) Change from baseline in Pain Visual Analog Scale (VAS) score at Week 4; (e) Change from baseline in Psoriasis Symptom Scale (PSS) score at Week 4;
(f) Change from baseline in Functional Assessment of Chronic Illness Therapy (FACIT) Fatigue score at Week 4; (g) GPPGA 0 or 1 at Week 4; (h) GPPGA pustulation subscore of 0 indicating no visible pustules at Week 4; (i) GPPASI 50 at Week 1 and 4; or (j) Change in GPPASI pustule, erythema or scaling severity subscore from baseline at Week 1 and 4. In a related embodiment, proportion of patients with a response to the administration is statistically significantly higher as compared to patients on placebo for any of the end points recited.

Pharmaceutical Compositions and Administration Thereof

[000155] The antibodies of the present invention can be administered either alone or in combination with other agents. Examples of antibodies for use in such pharmaceutical compositions are those that comprise an antibody or antibody fragment having the light chain variable region amino acid sequence of any of SEQ
ID NO: 1-10. Examples of antibodies for use in such pharmaceutical compositions are also those that comprise a humanized antibody or antibody fragment having the heavy chain variable region amino acid sequence of any of SEQ ID NO: 11-20.

[000156] Further examples of antibodies for use in such pharmaceutical compositions are also those that comprise a humanized antibody or antibody fragment having the light chain variable region amino acid sequence of any of SEQ ID
NO:76-86. Preferred antibodies for use in such pharmaceutical compositions are also those that comprise a humanized antibody or antibody fragment having the heavy chain variable region amino acid sequence of any of SEQ ID NO:87-101.

[000157] Further examples of antibodies for use in such pharmaceutical compositions are also those that comprise a humanized antibody or antibody fragment having the light chain variable region and heavy chain variable region of any of SEQ
ID NO: 77 and 89, SEQ ID NO: 80 and 88, SEQ ID NO: 80 and 89, SEQ ID NO: 77 and 87, SEQ ID NO: 77 and 88, SEQ ID NO: 80 and 87, SEQ ID NO: 86 and 100, SEQ ID NO: 85 and 101, or SEQ ID NO: 85 and 10.

[000158] Further examples of antibodies for use in such pharmaceutical compositions are also those that comprise a humanized antibody having the light chain region amino acid sequence of any of SEQ ID NO:115, 118, 123 or 124.
Preferred antibodies for use in such pharmaceutical compositions are also those that comprise humanized antibody having the heavy chain variable region amino acid sequence of any of SEQ ID NO:125, 126, 127, 138 or 139.

[000159] Further examples of antibodies for use in such pharmaceutical compositions are also those that comprise Antibody B1, Antibody B2, Antibody B3, Antibody B4, Antibody B5, Antibody B6, Antibody Cl, Antibody C2 or Antibody C3.

[000160] Various delivery systems are known and can be used to administer the IL-36R binding agent. Methods of introduction include but are not limited to intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, epidural, and oral routes. The IL-36R binding agent can be administered, for example by infusion, bolus or injection, and can be administered together with other biologically active agents such as chemotherapeutic agents. Administration can be systemic or local. In preferred embodiments, the administration is by subcutaneous injection.
Formulations for such injections may be prepared in for example prefilled syringes that may be administered once every other week.

[000161] In one aspect, the invention provides an article of manufacture comprising a subcutaneous administration device, which delivers to a patient a fixed dose of an antibody of the present invention. In some embodiments, the subcutaneous administration device is a pre-filled syringe, an autoinjector, or a large volume infusion device. For example, MyDose TM product from Roche, a single use infusion device that enables the subcutaneous administration of large quantities of liquid medication, may be used as the administration device. Numerous reusable pen and autoinjector delivery devices have applications in the subcutaneous delivery of a pharmaceutical composition of the present invention. Examples include, but are not limited to AUTOPENTm (Owen Mumford, Inc., Woodstock, UK), DISETRONICTm pen (Disetronic Medical Systems, Bergdorf, Switzerland), HUMALOG MIX 75/25TM pen, HUMALOGTm pen, HUMALIN 70/3OTM pen (Eli Lilly and Co., Indianapolis, Ind.), NOVOPENTM I, ll and III (Novo Nordisk, Copenhagen, Denmark), NOVOPEN JUNIORTM (Novo Nordisk, Copenhagen, Denmark), BDTM pen (Becton Dickinson, Franklin Lakes, N.J.), OPTIPENTm, OPTIPEN PROTM, OPTIPEN STARLETTm, and OPTICLIKTm (Sanofi-Aventis, Frankfurt, Germany), to name only a few. Examples of disposable pen delivery devices having applications in subcutaneous delivery of a pharmaceutical composition of the present invention include, but are not limited to the SOLOSTARTm pen (Sanofi-Aventis), the FLEXPENTM (Novo Nordisk), and the KWIKPENTM (Eli Lilly), the SURECLICKTM Autoinjector (Amgen, Thousand Oaks, Calif.), the PENLETTm (Haselmeier, Stuttgart, Germany), the EPIPEN (Dey, L.P.), and the HUMIRATm Pen (Abbott Labs, Abbott Park III.), YPSOMATETm, YPSOMATE 2.25TM, VAIROJECTTm (Ypsomed AG, Burgdorf, Switzerland) to name only a few. Additional information relating to example delivery devices that could be used with an antibody of the present invention may be found, for example, in CH705992A2, W02009/040602, W02016/169748, W02016/179713.

[000162] In specific embodiments, the IL-36R binding agent composition is administered by injection, by means of a catheter, by means of a suppository, or by means of an implant, the implant being of a porous, non-porous, or gelatinous material, including a membrane, such as a silastic membrane, or a fiber.
Typically, when administering the composition, materials to which the anti-IL-36R
antibody or agent does not absorb are used.

[000163] In other embodiments, the anti-IL-36R antibody or agent is delivered in a controlled release system. In one embodiment, a pump may be used (see, e.g., Langer, 1990, Science 249:1527-1533; Sefton, 1989, CRC Crit. Ref. Biomed. Eng.

14:201; Buchwald et al., 1980, Surgery 88:507; Saudek et al., 1989, N. Engl.
J. Med.
321:574). In another embodiment, polymeric materials can be used. (See, e.g., Medical Applications of Controlled Release (Langer and Wise eds., CRC Press, Boca Raton, Fla., 1974); Controlled Drug Bioavailability, Drug Product Design and Performance (Smolen and Ball eds., Wiley, New York, 1984); Ranger and Peppas, 1983, Macromol. Sci. Rev. Macromol. Chem. 23:61. See also Levy et al., 1985, Science 228:190; During et al., 1989, Ann. Neural. 25:351; Howard et al., 1989, J.
Neurosurg. 71:105.) Other controlled release systems are discussed, for example, in Langer, supra.

[000164] An IL-36R binding agent (e.g., an anti-IL-36R antibody) can be administered as pharmaceutical compositions comprising a therapeutically effective amount of the binding agent and one or more pharmaceutically compatible ingredients.

[000165] In one embodiment, the anti-IL-36R antibody or an antigen binding fragment thereof (disclosed herein) is present in a pharmaceutical formulation (as described in co-pending PCT publication No. 20200185479, published on September 17, 2020, the entire content of which is hereby incorporated herein by reference in its entirety) suitable for administration to a mammal or patient according to any one of the aspects described herein. Various examples to this embodiment are described as numbered clauses (1, 2, 3, etc.) below for convenience. These are provided as examples and do not limit the subject technology. It is noted that any of the dependent clauses may be combined in any combination, and placed into a respective independent clause, e.g., clause 1. The other clauses can be presented in a similar manner.
1. A method of treating generalized pustular psoriasis (GPP) flares in a patient, said method comprising administering to the patient two 900 mg intravenous (i.v.) doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.
2. A method of treating GPP in a patient by administering to the patient a single 900 mg intravenous dose of an anti-IL-36R antibody, if the GPP symptoms persist, administering an additional 900 mg intravenous dose an anti-IL-36R
antibody one week after the initial dose.
3. A method of treating GPP in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody;
wherein the second dose is administered less than 2 weeks after the first dose.
4. A method of reducing or alleviating signs and symptoms of an acute phase flare-up of GPP in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.
5. A method of reducing the severity and duration of GPP symptoms, said method comprising including administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.
6. A method of treating a skin disorder associated with GPP, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.
7. A method of preventing the recurrence of GPP flares in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.
8. A method of reducing pain by at least 10% in a patient with GPP, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.
9. A method of improving the quality of life by at least 10% in a patient with moderate to severe GPP symptoms, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.
10. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of 2.
11. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2.
12. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before the administration of the first i.v. dose.

13. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 after the administration of the first i.v. dose.
14. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before and after the administration of the first i.v.
dose.
15. The method according to any of the preceding clauses, wherein the second dose is administered after 1 week but less than 2 weeks from the first dose.
16. A method of treating a GPP patient with a GPPGA pustulation subscore of 2, said method comprising the steps of: (a) administering to the patient a first mg intravenous (I.V.) dose of an anti-IL-36R antibody; (b) assessing the GPPGA pustulation subscore of the patient and if the GPPGA pustulation subscore of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.
17. A method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of 2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (i.v.) dose of an anti-IL-36R antibody; (b) assessing the GPPGA total score of the patient and if the GPPGA total score of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.
18. A method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA pustulation subscore of 2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (I.V.) dose of an anti-IL-36R antibody; (b) assessing the GPPGA
scores of the patient and if the GPPGA total score of 2 and the GPPGA

pustulation subscore of 2 of the patient persist after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.
19. The method according to any of the preceding clauses, wherein an optional third 900 mg i.v. dose of the anti-IL-36R antibody is administered at 2 to 12 weeks after the second i.v. dose.
20. The method according to any of the preceding clauses, wherein the two-dose administration achieves one or more of the following results:
(a) a Generalized Pustular Psoriasis Global Assessment (GPPGA) pustulation subscore of 0 indicating in one week after administering the second i.v. dose;

and/or (b) a GPPGA total score of 0 or 1 in one week after administering the second i.v. dose.
21. The method of clause 20, wherein the results are maintained for up to and at least 12 weeks following the administration of the second i.v. dose.
22. The method according to any of the preceding clauses, wherein the method further comprises administering to the patient a prophylactically effective amount of the anti-IL-36R antibody in one or more subcutaneous doses after the last i.v. dose administered.
23. The method according clause 22, wherein each of the one or more subcutaneous doses comprises 150 mg, 225 mg, 300 mg, 450 mg, or 600 mg of said anti-IL-36R antibody.
24. The method according to clause 23, wherein 1, 2, 3 or more subcutaneous doses are administered to the patient and wherein a first subcutaneous dose is administered after the last intravenous dose.
25. The method according to clause 24, wherein a first subcutaneous dose is administered 2 to 8 weeks, 4 to 6 weeks, 2 weeks, 4 weeks, 6 weeks or 8 weeks, 12 weeks, 16 weeks, 20 weeks after the last intravenous dose, and subsequent subcutaneous doses are administered at 2, 4, 6, 8, 10 or 12 weeks intervals after the first subcutaneous dose.
26. The method according to any of the preceding clauses, wherein the patient remains in clinical remission as measured by a GPPGA total score of 0 or 1 for at least 12, 24, 36, 48, 60 or 72 weeks following the last i.v. or subcutaneous dose.
27. The method according to any of the preceding clauses, wherein the anti-antibody comprises: a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 35, 102, 103, 104, 105 106 or 140 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
28. The method according to any of the preceding clauses, wherein the anti-antibody comprises:
I. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 102 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
II. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 103 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
III, a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 104 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
IV. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 105 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
V. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 106 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
VI. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 140 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
29. The method according to any of the preceding clauses, wherein the anti-antibody comprises:
(i) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 87;or (ii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 88; or (iii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 89; or (iv) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 87;or (v) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 88; or (vi) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 89; or (vii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 85; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 100; or (viii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 85; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:101; or (ix) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 86; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 100; or (x) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 86; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:101.
30. The method according to any of the preceding clauses, wherein the anti-IL-36R
antibody comprises:
i. a light chain comprising the amino acid sequence of SEQ ID NO: 115;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
125; or ii. a light chain comprising the amino acid sequence of SEQ ID NO: 115;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
126; or iii. a light chain comprising the amino acid sequence of SEQ ID NO: 115;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
127; or iv. a light chain comprising the amino acid sequence of SEQ ID NO: 118;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
125; or v. a light chain comprising the amino acid sequence of SEQ ID NO: 118;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
126; or vi. a light chain comprising the amino acid sequence of SEQ ID NO: 118;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
127; or vii. a light chain comprising the amino acid sequence of SEQ ID NO: 123;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
138; or viii. a light chain comprising the amino acid sequence of SEQ ID NO: 123;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
139; or ix. a light chain comprising the amino acid sequence of SEQ ID NO: 124;
and a heavy chain comprising the amino acid sequence of SEQ ID NO:
138.
31. The method according to any of the preceding clauses, wherein the anti-antibody is spesolimab.
32. A method of treating generalized pustular psoriasis (GPP) flares in a patient, said method comprising administering to the patient two intravenous (i.v.) doses of 900 mg spesolimab; wherein the second dose is administered less than 2 weeks after the first dose.
33. A method of treating GPP in a patient, said method comprising administering to the patient two intravenous doses of 900 mg spesolimab; wherein the second dose is administered less than 2 weeks after the first dose.
34. A method of reducing or alleviating signs and symptoms of an acute phase flare-up of GPP in a patient, said method comprising administering to the patient two intravenous doses of 900 mg spesolimab; wherein the second dose is administered less than 2 weeks after the first dose.
35. A method of reducing the severity and duration of GPP symptoms, said method comprising including administering to the patient two intravenous doses of 900 mg spesolimab; wherein the second dose is administered less than 2 weeks after the first dose.
36. A method of treating a skin disorder associated with GPP, said method comprising administering to the patient two intravenous doses of 900 mg spesolimab; wherein the second dose is administered less than 2 weeks after the first dose.
37. A method of preventing the recurrence of GPP flares in a patient, said method comprising administering to the patient two intravenous doses of 900 mg spesolimab; wherein the second dose is administered less than 2 weeks after the first dose.
38. A method of reducing pain by at least 10% in a patient with GPP, said method comprising administering to the patient two intravenous doses of 900 mg spesolimab; wherein the second dose is administered less than 2 weeks after the first dose.
39. A method of improving the quality of life by at least 10% in a patient with moderate to severe GPP symptoms, said method comprising administering to the patient two intravenous doses of 900 mg spesolimab; wherein the second dose is administered less than 2 weeks after the first dose.
40. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of 2.
41. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2.
42. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before the administration of the first i.v. dose.

43. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 after the administration of the first i.v. dose.
44. The method according to any of the preceding clauses, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before and after the administration of the first i.v.
dose.
45. The method according to any of the preceding clauses, wherein the second dose is administered after 1 week but less than 2 weeks from the first dose.
46. A method of treating a GPP patient with a GPPGA pustulation subscore of 2, said method comprising the steps of: (a) administering to the patient a first intravenous (i.v.) dose of 900 mg spesolimab; (b) assessing the GPPGA
pustulation subscore of the patient and if the GPPGA pustulation subscore of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second (i.v.) dose of 900 mg spesolimab less than 2 weeks after the first dose.
47. A method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of 2, said method comprising the steps of: (a) administering to the patient a first intravenous (i.v.) dose of 900 mg spesolimab;
(b) assessing the GPPGA total score of the patient and if the GPPGA total score of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second (i.v.) dose of 900 mg spesolimab less than 2 weeks after the first dose.
48. A method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA pustulation subscore of 2, said method comprising the steps of: (a) administering to the patient a first intravenous (I.V.) dose of 900 mg spesolimab; (b) assessing the GPPGA
scores of the patient and if the GPPGA total score of 2 and the GPPGA

pustulation subscore of 2 of the patient persist after 1 week from the first dose, then administering to the patient a second (i.v.) dose of 900 mg spesolimab less than 2 weeks after the first dose.
49. The method according to any of the preceding clauses, wherein an optional third i.v. dose of 900 mg spesolimab is administered at 2 to 12 weeks after the second i.v. dose.
50. The method according to any of the preceding clauses, wherein the two-dose administration achieves one or more of the following results:
(a) a Generalized Pustular Psoriasis Global Assessment (GPPGA) pustulation subscore of 0 indicating in one week after administering the second i.v. dose;

and/or (b) a GPPGA total score of 0 or 1 in one week after administering the second i.v. dose.
51. The method of clause 50, wherein the results are maintained for up to and at least 12 weeks following the administration of the second i.v. dose.
52. The method according to any of the preceding clauses, wherein the method further comprises administering to the patient a prophylactically effective amount of spesolimab in one or more subcutaneous doses after the last i.v.
dose administered.
53. The method according clause 52, wherein each of the one or more subcutaneous doses comprises 150 mg, 225 mg, 300 mg, 450 mg, or 600 mg spesolimab.
54. The method according to clause 53, wherein 1, 2, 3 or more subcutaneous doses are administered to the patient and wherein a first subcutaneous dose is administered after the last intravenous dose.
55. The method according to clause 54, wherein a first subcutaneous dose is administered 2 to 8 weeks, 4 to 6 weeks, 2 weeks, 4 weeks, 6 weeks or 8 weeks, 12 weeks, 16 weeks, 20 weeks after the last intravenous dose, and subsequent subcutaneous doses are administered at 2, 4, 6, 8, 10 or 12 weeks intervals after the first subcutaneous dose.
56. The method according to any of the preceding clauses, wherein the patient remains in clinical remission as measured by a GPPGA total score of 0 or 1 for at least 12, 24, 36, 48, 60 or 72 weeks following the last i.v. or subcutaneous dose.
57. The method according to any of the preceding clauses, wherein the patient experiences an improvement in Psoriasis Symptom Scale (PSS) score within 1, 4 or 12 weeks after receiving the dose(s) of spesolimab; wherein the improvement is at least 10% when the PSS score after the anti-IL-36R antibody or spesolimab administration is compared to the PSS score at baseline in the patient before the administration.
58. The method according to any of the preceding clauses, wherein the patient experiences an improvement in Functional Assessment of Chronic Illness Therapy - Fatigue (FACIT-Fatigue) score within 1, 4 or 12 weeks after receiving the dose(s) of spesolimab; wherein the improvement is at least 10% when the FACIT-Fatigue score after the anti-IL-36R antibody or spesolimab administration is compared to the FACIT-Fatigue score at baseline in the patient before the administration.
59. The method according to any of the preceding clauses, wherein the patient experiences an improvement in Dermatology Quality of Life Index (DLQI), Psoriasis System Scale (PSS), Visual Analogue Scale (VAS) or Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-Fatigue) score within 1, 4 or 12 weeks after receiving the dose(s) of spesolimab; wherein the improvement is at least 10% when the DQLI, PSS, VAS or FACIT-Fatigue score after the anti-IL-36R antibody or spesolimab administration at week 1, 4 or 12 is compared to the DQLI, PSS, VAS or FACIT-Fatigue score at baseline in the patient before the administration.

60. A method of improving Psoriasis Symptom Scale (PSS), Dermatology Quality of Life Index (DLQI), Visual Analogue Scale (VAS) or Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-Fatigue) score in a patient with GPP, said method comprising administering to the patient one 900 mg intravenous (i.v.) dose of spesolimab and optional second 900 mg i.v. dose of spesolimab; wherein the second optional dose of spesolimab is administered less than 2 weeks after the first dose if the GPP symptoms (e.g., a GPPGA
total score of and/or a GPPGA pustulation subscore of 2) in the patient persist.
61. A method of improving Functional Assessment of Chronic Illness Therapy -Fatigue (FACIT-Fatigue) score in a patient with GPP, said method comprising administering to the patient one 900 mg intravenous (i.v.) dose of spesolimab and optional second 900 mg i.v. dose of spesolimab; wherein the second optional dose of spesolimab is administered less than 2 weeks after the first dose if the GPP symptoms (e.g., a GPPGA total score of and/or a GPPGA
pustulation subscore of 2) in the patient persist.
62. A method of improving Dermatology Quality of Life Index (DLQI) score in a patient with GPP, said method comprising administering to the patient one 900 mg intravenous (i.v.) dose of spesolimab and optional second 900 mg i.v. dose of spesolimab; wherein the second optional dose of spesolimab is administered less than 2 weeks after the first dose if the GPP symptoms (e.g., a GPPGA
total score of and/or a GPPGA pustulation subscore of 2) in the patient persist.
63. The method according to any of clauses 59 to 61, wherein the improvement is at least 10% when the score after the spesolimab administration at week 1, 4 or 12 is compared to the score at baseline in the patient before the administration.

64.
The method according to any of the preceding clauses, wherein the spesolimab administration(s) result(s) in a lower maximum ADA titers at week(s) 2, 4, 6, 8, and/or 12 of the treatment.

[000166]
Further, the pharmaceutical composition can be provided as a pharmaceutical kit comprising (a) a container containing a IL-36R binding agent (e.g., an anti-IL-36R antibody) in lyophilized form and (b) a second container containing a pharmaceutically acceptable diluent (e.g., sterile water) for injection. The pharmaceutically acceptable diluent can be used for reconstitution or dilution of the lyophilized anti-IL-36R antibody or agent. Optionally associated with such container(s) can be a notice in the form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceuticals or biological products, which notice reflects approval by the agency of manufacture, use or sale for human administration.

[000167]
Such combination therapy administration can have an additive or synergistic effect on disease parameters (e.g., severity of a symptom, the number of symptoms, or frequency of relapse).

[000168]
With respect to therapeutic regimens for combinatorial administration, in a specific embodiment, an anti-IL-36R antibody or IL-36R binding agent is administered concurrently with a therapeutic agent. In another specific embodiment, the therapeutic agent is administered prior or subsequent to administration of the anti-IL-36R
antibody or IL-36R binding agent, by at least an hour and up to several months, for example at least an hour, five hours, 12 hours, a day, a week, a month, or three months, prior or subsequent to administration of the anti-IL-36R antibody or IL-36R binding agent.

[000169]
The invention is further described in the following examples, which are not intended to limit the scope of the invention.
Examples Example 1: Trial of Spesolimab for Generalized Pustular Psoriasis ABSTRACT

[000170] Background: Generalized pustular psoriasis is a rare, life-threatening, inflammatory skin disease characterized by widespread eruption of sterile pustules.
Dysregulated interleukin-36 signaling is involved in its pathogenesis. We compared spesolimab, a humanized anti-interleukin-36 receptor monoclonal antibody with placebo in a phase 2 trial in patients with flares of generalized pustular psoriasis.

[000171] Methods: Patients with a flare of generalized pustular psoriasis were randomly assigned in a 2:1 ratio to a single 900 mg intravenous dose of spesolimab or placebo. The primary endpoint was a Generalized Pustular Psoriasis Physician Global Assessment (GPPGA) pustulation subscore of 0 (range 0 to 4, 0 indicating no pustules and 4 indicating severe pustulation) at week 1. The key secondary endpoint was a GPPGA total score of 0 or 1 (clear or almost clear) at week 1 (range 0 to 4, higher scores indicating greater disease severity).

[000172] Results: A total of 85 patients were screened and 53 were enrolled: 35 were assigned to receive spesolimab and 18 to receive placebo. Baseline GPPGA
pustulation subscores were 3 in 46% and 39%, and 4 in 37% and 33%, of the spesolimab and placebo groups, respectively. At the end of week 1, a pustulation subscore of 0 was achieved in 19/35 (54%) of patients receiving spesolimab versus 1/18 (6%) receiving placebo (difference, 49 percentage points; 95% confidence interval [Cl] 21.5 to 67.2]; P<0.001). At the end of week 1, a GPPGA total score of 0 or 1 was achieved by 15/35 (43%) of patients in the spesolimab group and by (11%) of patients in the placebo group (difference, 32 percentage points; 95%
Cl, 2.2 to 52.7]; P=0.024). Drug reactions with eosinophilia and systemic symptoms were reported in two spesolimab-treated patients. Infections occurred in 17% of spesolimab-treated patients at week 1. Anti-drug antibodies were detected in (46%) patients who received at least one dose of spesolimab.

[000173] Conclusions: In a 1 week phase 2 trial in patients with a flare of generalized pustular psoriasis, the IL-36 receptor inhibitor spesolimab, resulted in higher rates of lesion clearance than placebo but was associated with more infections and systemic drug reactions. Longer and larger trials are required to determine the effect and risks of spesolimab in pustular psoriasis. (Funded by Boehringer Ingelheim;
ClinicalTrials.gov identifier: NCT03782792).
INTRODUCTION

[000174] Generalized pustular psoriasis (GPP) is a rare, potentially life-threatening, autoinflammatory skin disease characterized by widespread eruption of sterile, visible pustules that occurs with or without systemic symptoms of pain, fever, general malaise, fatigue, and extracutaneous manifestations such as arthritis and neutrophilic cholangitis. The clinical course of GPP can be relapsing with recurrent flares, or persistent with intermittent flares. Mortality rates range from 2% to 16% and have been attributed to septic shock and cardiorespiratory failure. The frequency of flares varies among patients, and flares may be spontaneous or triggered by upper respiratory tract infections, stress, medication, medication withdrawal and pregnancy. The disease has an adverse impact on quality of life.

[000175] There are no approved therapies for the disease in the United States or Europe and management has included cyclosporine, retinoids, methotrexate and biologics. Biologic agents that inhibit tumor necrosis factor-alpha (adalimumab, infliximab, certolizumab pegol), interleukin (IL)-17/1L-17 receptor (secukinumab, brodalumab, ixekizumab), and IL-23 (risankizumab, guselkumab) are approved for use in Japan, Taiwan and Thailand based on small trials and studies of these drugs in plaque psoriasis and small non-randomized trials in GPP.

[000176] The role of the IL-36 pathway in GPP is supported by the finding of loss-of-function mutations in the IL-36 receptor antagonist gene (IL36RN), and associated genes (CARD14, AP1S3, SERPINA3, MPO), and the overexpression of IL-36 cytokines in GPP skin lesions. Clinical improvements with spesolimab, a humanized anti-IL-36 receptor monoclonal antibody, were observed in an open-label phase study in seven patients presenting with a GPP flare.

[000177] We conducted a phase 2 randomized trial to investigate the efficacy and safety of spesolimab versus placebo in patients presenting with a GPP flare.
Because acute and severe flares of this disorder are life-threatening, a single dose of the drug with a placebo-controlled period of 1 week was chosen for the trial design and patients from both arms were offered the opportunity to receive open-label spesolimab on day 8. At week 12, the end of the trial, patients were offered enrolment into a separate open-label extension trial of spesolimab (ClinicalTrials.gov identifier:
NCT03886246).
METHODS
Trial design

[000178]
This phase 2, multicenter, randomized, double-blind, placebo-controlled trial was conducted between 20 February 2019 and 5 January 2021, and enrolled patients from 37 sites in 12 countries. Patients presenting with a GPP flare were randomly assigned in a 2:1 ratio to receive a single intravenous dose of 900 mg spesolimab or placebo (Figure 1). Randomization was performed using an interactive response system with stratification factor of Japanese race versus non-Japanese race. Patients and investigators were masked to treatment group assignment administered on day 1 throughout the trial until the database was locked for analyses.
On day 8, patients from both arms were eligible to receive an open-label, single, intravenous dose of 900 mg spesolimab (which led to a cross-over from placebo to spesolimab for some patients) if they had persistent symptoms based on a pre-defined threshold that consisted of a GPP Physician Global Assessment (GPPGA) total score at the end of week 1 (range from 0 [clear skin] to 4 [severe disease]) and a clinician assessment of GPP severity based on a modified PGA and a GPPGA pustulation subscore at week 1 (range from 0 [no visible pustules] to 4 [severe pustulation].
The GPPGA total score is the average of the sub-scores [pustulation, erythema, and scaling]; (see the Supplementary information below). After week 1, rescue treatment with a single intravenous dose of 900 mg spesolimab could be administered in case of reoccurrence of a flare (defined as point increase in both the GPPGA total score and the pustulation subscore after first attaining a GPPGA total score of 0 or 1).
Patients who achieved clinical improvement and completed the trial without flare symptoms were eligible to enter a 5-year open-label extension trial (ClinicalTrials.gov identifier: NCT03886246). Escape treatment with standard of care, per the treating physician's choice, was allowed for patients who experienced worsening of disease that required immediate treatment during week 1, and for patients with disease worsening who did not qualify for a rescue medication with open-label spesolimab after week 1. The use of escape treatment in the first week was essential for patient safety because flares of GPP are potentially life threatening. Any patient who received escape medication was considered not achieving a response (non response) in the analysis for the primary and key secondary evaluation at week 1. Details of the trial design are in the protocol described below and in Figure 2.

[000179] Patients aged 18-75 years were eligible for enrollment if they had a history of GPP consistent with criteria for diagnosis according to European Rare And Severe Psoriasis Expert Network (ERASPEN) criteria1. Analyses of coding sequences for the three main GPP-associated genes (IL36RN, CARD14, AP1S3) were performed on DNA extracts from blood samples and patients were enrolled without regard to mutation status. Patients had to have a GPP flare of moderate-to-severe intensity (defined as total GPPGA score 3, new or worsening pustules, a GPPGA
pustulation subscore 2, and 5 /0 body surface area with erythema and the presence of pustules). Key exclusion criteria included patients with plaque psoriasis without pustules or with pustules restricted to psoriatic plaques, drug-triggered acute generalized exanthematous pustulosis, immediate life-threatening flare of GPP
requiring intensive care treatment, and requirement for current treatment with methotrexate, cyclosporine, or retinoids, or any restricted medication.
Endpoints

[000180] The primary endpoint was the achievement of a GPPGA pustulation subscore of 0 (clear) at the end of week 1. The key secondary endpoint was a GPPGA
score of 0 or 1 (clear or almost clear) at the end of week 1. Secondary endpoints were primarily at week 4, when some patients might have received open-label spesolimab on day 8. Secondary endpoints at week 4 included: a 75% or greater improvement in Psoriasis Area and Severity Index for Generalized Pustular Psoriasis (GPPASI
75;

GPPASI is an adaptation of the PASI score in which induration is replaced by a pustule component; scores range from 0 [least severe] to 72 [most severe], change from baseline in visual analog pain scale (VAS, range from 0 [no pain] to 100 [severe pain]), change from baseline in Psoriasis Symptom Scale (PSS; patient-reported psoriasis pain, redness, itching, and burning; range from 0 to 16, with higher scores indicating more severe symptoms), and change from baseline in Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-Fatigue; patient-reported impact of fatigue on daily activities; range from 0 to 52, with lower scores indicating greater impact). There were two additional secondary endpoints assessed at week 1 and/or 4 as listed below and the protocol and statistical analysis plan.

[000181] Details of trial outcomes, as well as a list of exploratory endpoints are listed in Supplementary Table S3 below.

[000182] Safety events at week 1 (through the first 8 days of the study) and through week 12 included treatment-emergent and serious adverse events. Adverse events were assessed by the trial investigators, who were masked to treatment assignments until after the database lock for the final analysis of the trial. During the course of the trial, adverse events occurring were collected, documented on the electronic case reports, and reported to the sponsor by the investigator. The intensity of the adverse events was assessed by the investigators and graded according to RCTC Version 2.0 developed by the Outcome Measures in Rheumatology (OMERACT) organization.
Statistical analysis

[000183] A sample size of 51 patients was estimated to provide 90 /0 power to detect any difference between spesolimab and placebo with assumed response rates of 0.6 and 0.1, respectively, for both the primary and key secondary endpoints, and a type I
error of <0.025 (one-sided), which can be considered as a type I error of <0.05 with a two-sided test. The primary endpoint and key secondary endpoint were analyzed using the randomized set with an exact Suissa¨Shuster Z-pooled test. This is a one-sided test; two-sided P-value was reported by doubling the one-sided P-value.30 Confidence intervals (95% Cl) around the risk difference were calculated using the Chan and Zhang method for the primary and all binary secondary endpoints. The fixed sequence test was used to control the familywise type I error. The primary and key secondary endpoints (both assessed at day 8 [week 1]) were tested in a hierarchical manner at a two-sided level of P<0.05. If the primary endpoint failed to reach a significant difference between the trial groups, the key secondary outcome would not be tested. The protocol and statistical analysis plan called for hierarchical testing of 4 subsequent secondary endpoints (GPPASI 75 and change from baseline in: pain VAS;
PSS; and FACIT-Fatigue), all at week 4; however, randomization to trial groups no longer pertained after week 1 as 15 of 18 patients assigned to placebo received open-label spesolimab on day 8 and were imputed with non-response or the worst possible outcome. Therefore, comparisons by randomized treatment as originally planned were non-informative and the changes at week 1 and 4 for these endpoints are reported descriptively by the following groups: All patients randomized to spesolimab (patients who received one or two doses), patients randomized to a single dose of spesolimab only (patients received spesolimab on day 1 only), patients randomized to spesolimab who received open-label day 8 spesolimab (Patients who qualified for a second dose on day 8), and patients randomized to placebo who received open-label spesolimab on day 8 (patients who received one dose of spesolimab on day 8).
For Binary outcomes, patients with missing data were considered not to have reached the respective endpoint. For continuous outcomes, last observed case carried forward was used for imputation.

[000184] Post hoc sensitivity analyses of the primary and key secondary endpoints using linear regression with adjustment for the imbalanced covariates at baseline, including gender, race and GPPASI were performed but no conclusions can be drawn from these data.
RESULTS
Patients

[000185] Of 85 patients screened, 53 were enrolled: 35 assigned to 900 mg spesolimab and 18 to placebo (Figure 1; Table 1). Baseline demographic and disease characteristics differed between arms in respect to the percentage of female participants (60.0% and 83.3%, in the spesolimab and placebo arms, respectively) and the proportion of Asian participants (45.7% and 72.2%, respectively).
Baseline median GPPASI total score was 27.4 in the spesolimab arm and 20.9 in the placebo arm (Table 1). At the time of randomization, 18.9% of patients had a GPPGA
total score of 4 (severe), and most patients had a GPPGA pustulation subscore of 3 or 4 (high or very high density of pustules) and impaired quality of life and clinical burden as indicated by Dermatology Life Quality Index (DLQI), pain VAS, FACIT-Fatigue, and PSS (Supplementary Table S3). Seven patients, five in the spesolimab arm and two in the placebo arm, had IL36RN mutations (Table 1). Most patients had no (38 patients without) or AP1S3 (42 without) mutations (Supplementary Table S4).
Table 1. Demographics and Baseline Characteristics for Current Flare Characteristic Spesolimab Placebo (N=35) (N=18) Mean age (SD) - yr 43.2 (12.1) 42.6 (8.4) Mean weight (SD) - kg 73.7 (24.0) 68.8 (26.6) Female - no. (%) 21 (60.0) 15 (83.3) Asian race* - no. (%) 16 (45.7) 13 (72.2) White race* - no. (%) 19 (54.3) 5 (27.8) GPPGA total score.' 3 - no. (%) 28 (80.0) 15 (83.3) 4- no. (%) 7 (20.0) 3 (16.7) GPPGA pustulation subscore 2- no. (%) 6 (17.1) 5 (27.8) 3 - no. (%) 16 (45.7) 7 (38.9) 4- no. (%) 13 (37.1) 6 (33.3) Median GPPASI total score 27.4 (15.5-36.8) 20.9 (12.0-32.0) (IQR) IL36RN mutationll Yes- no. (%) 5(14.3) 2(11.1) No - no. (%) 24 (68.6) 12 (66.7) *Race was reported by the patient.
tGeneralized Pustular Psoriasis Physician Global Assessment (GPPGA) scores range from 0 (clear skin) to 4 (severe disease).

tGPPGA pustulation subscores range from 0 (no visible pustules) to 4 (very high-density pustules with pustular lakes).
Psoriasis Area and Severity Index for Generalized Pustular Psoriasis (GPPASI) scores range from 0 (least severe) to 72 (most severe).
'Five patients had homozygous mutations (four p.Leu27Pro; one p.Ser113Leu), and two patients had heterozygous mutations (p.Ser113Leu; p.Ser113Leu/p.Va144Met). At the date of database lock (18 January 2021), DNA sequencing (targeted re-sequencing IIlumina Miseq, Illumina Inc., San Diego, CA) was not yet completed in three patients and there were missing samples from seven patients.
IQR, interquartile range; SD, standard deviation.

[000186] A total of 52 of the 53 enrolled patients completed the first week of the trial.
Data for one patient in the spesolimab arm was missing for primary and key secondary endpoints and was imputed to no response. At day 8, 12 patients (34.3%) in the spesolimab group and 15 patients (83.3%) in the placebo group received an open-label dose of spesolimab. After day 8, 32 patients (91.4%) initially randomized to spesolimab and 17 patients (94.4%) initially randomized to placebo completed the 12-week follow-up period, during which four and two patients, respectively, required rescue treatment with spesolimab. After completing 12 weeks of treatment, 39 patients enrolled into the open-label extension trial (Figure 1).
Efficacy Primary and key secondary efficacy endpoints

[000187] At the end of week 1, 19 patients (54.3%) randomized to spesolimab versus one patient (5.6%) randomized to placebo achieved a GPPGA pustulation subscore of 0 (no visible pustules); (difference, 48.7 percentage points; 95%
confidence interval [Cl], 21.5 to 67.2; P<0.001; Table 2, Figure 3A). A GPPGA total score of 0 or 1 (clear or almost clear skin) at week 1 was achieved in 15 patients (42.9%) randomized to spesolimab versus two patients (11.1%) randomized to placebo (difference, 31.7 percentage points; 95% Cl, 2.2 to 52.7; P=0.024; Table 2, Figure 3B). The GPPGA
pustulation subscores and the GPPGA total scores over time only considering randomized treatment at day 1 are shown in Figures 4 and 5.
Table 2. Primary and Key Secondary Efficacy Endpoints Spesolimab Placebo (N=35) (N= 18) Primary endpoint GPPGA pustulation subscore of 0 at week 1 Responders ¨ no. (%) 19 (54.3) 1 (5.6) Difference vs placebo 48.7 percentage points (95% Cl) (21.5 to 67.2) P-value* <0.001 Key secondary endpoint GPPGA total score of 0 or 1 at week 1 Responders ¨ no. (%) 15 (42.9) 2 (11.1) Difference vs placebo 31.7 percentage points (95% Cl) (2.2 to 52.7) P-value* 0.024 P-value* 0.024 *By Suissa-Shuster Z-pooled test, two-sided P-value.
Cl, confidence interval; GPPGA, Generalized Pustular Psoriasis Physician Global Assessment.

[000188] Post-hoc sensitivity analyses of the primary and key secondary endpoints to adjust for the observed baseline imbalance in gender, race, and GPPASI were consistent with the primary analysis. (Supplementary Tables S5 and S6).
Exploratory efficacy endpoints post day 8 by randomized treatment at day 1 and open-label spesolimab treatment at day 8

[000189] After week 1, 15/18 patients assigned to placebo received open-label spesolimab on day 8, thus planned hierarchical testing of comparative secondary endpoints at week 4 were non-informative. Instead, the secondary endpoints were reported descriptively by four groups that reflected the treatment paths post day 8: All patients randomized to spesolimab (patients who received one [day 1 only] or two doses [day 1 plus day 8]; N=35), patients randomized to a single dose of spesolimab only (day 1; N=23), patients randomized to spesolimab who received open-label day 8 spesolimab (day 1 plus day 8; N=12), and patients randomized to placebo who received open-label spesolimab on day 8 (N=15). Descriptive analyses for GPPGA

pustulation subscore of 0 and GPPGA total score of 0 or 1 for these subgroups over time are reported in the Supplementary information (below) and Figures 6 and 7 and secondary endpoints including GPPASI 75 and change from baseline in: pain VAS, PSS, and FACIT-Fatigue at week 4 are reported in the Supplementary information below.

[000190] In addition, for the group of patients randomized to spesolimab who received one (day 1) or two doses (day 1 plus day 8; N=35) of spesolimab, descriptive results for GPPASI, GPPASI 75, pain VAS, DLQI, neutrophil counts, and C-reactive protein levels over 12 weeks are reported in Figure 8. Changes from baseline in GPPGA scores (including GPPGA pustulation, erythema and scaling/crusting subscores and GPPGA total score) in three patients after receiving a first and a second dose of spesolimab on days 1 and 8, respectively are shown in Figure 9.
Safety

[000191] Through the first week of randomized treatment, adverse events were reported in 65.7% of patients assigned to spesolimab and 55.6% in the placebo arm.
Pyrexia occurred in 5.7% and 22.2% of the patients receiving spesolimab and placebo, respectively; all pyrexia events occurred in the context of the underlying GPP
flare but the drug causing pyrexia cannot be excluded (Table 3). Infections were reported in 17.1% and 5.6% of patients in the spesolimab and placebo groups, respectively, through the first week (Supplementary Table S8); At week 1, in the spesolimab group, there were two cases of urinary tract infections, for all other infections, there was one case of each (see footnote to Supplementary Table S8).
Serious adverse events were reported in 5.7% of patients with spesolimab and none of the patients receiving placebo.
Table 3. Adverse Events Summary*

n (%) Week 1 Week 121 [rate/100 patient-years]
Spesolimab Placebo Spesolimab (N=35) (N=18) (N=51) Any adverse event 23 (65.7) 10 (55.6) 42 (82.4) [5874.7] [4623.4] [981.5]
Severe adverse event (RCTC 2 (5.7) 1 (5.6) 5 (9.8) grade 3 or 4) [309.5] [304.4] [40.9]
Investigator-defined drug-related 10 (28.6) 5 (27.8) 28 (54.9) adverse event [1747.6] [1773.1] [353.5]
Serious adverse event 2 (5.7) 0 (0) 6 (11.8) [309.5] [0] [49.7]
j Death 0 0 0 j Adverse event leading to 0 0 0 discontinuation of treatment ..
., Common adverse events , Pyrexia 2 (5.7) 4 (22.2) 5 (9.8) [313.5] [1404.8] [41.3]
Dizziness 0 2 (11.1) 0 [619.1] ..
..
.......................................................... i .......
Serious adverse events .......................................................... i .......
Drug reaction with eosinophilia and 1 (2.9) 0 2 (3.9) systemic symptom [154.1] [15.9]
Urinary tract 1 (2.9) 0 1 (2.0) infection [154.1] [7.8]
Drug-induced liver 1 (2.9) 0 1 (2.0) injury [154.1] [7.8]
..
Arthritis 1 (2.9) 0 1 (2.0) [152.2] [7.8]
..
Chronic plaque 0 0 1 (2.0) psoriasis worsening ll [7.8]
Influenza 0 0 1 (2.0) [7.7]
Squamous cell carcinoma of skin 0 0 1 (2.0) [7.7]

*All adverse events occurring between start of treatment and end of the residual effect period (16 weeks after the last dose of trial) were considered 'treatment-emergent'. Adverse events were coded using the Medical Dictionary for Drug Regulatory Activities version 23.1. The adverse event severity was graded according with the Rheumatology Common Toxicity Criteria (RCTC) version 2.0 safety analysis set. Pustular psoriasis was excluded as an adverse event from this safety analysis.
tDataset at week 12 includes patients randomized to spesolimab who received up to three doses of spesolimab and patients initially randomized to placebo who received open-label spesolimab at or post day 8. All adverse events from the first use of spesolimab to the residual effect period of the last spesolimab dose are included.
tCommon adverse events are reported in 0% of patients in any treatment group.
Drug-induced liver injury was reflected by an increase of transaminases and was considered a systemic symptom of drug reaction with eosinophilia and systemic symptom.
Chronic plaque psoriasis worsening capture events that were reflective of non-pustular psoriasis; these events were not captured in the efficacy outcomes.

[000192] At week 12, 82.4% of patients who received at least one dose of spesolimab (including those initially randomized to placebo who received open-label spesolimab at day 8) had an adverse event, and 11.8% had a serious adverse event; within the spesolimab group, the proportions of patients with adverse events remained unchanged or increased while the time-adjusted incidence rates decreased from week 1 to week 12 (Table 3). Infections were reported in 47.1% of patients; there were three cases each of urinary tract infection and influenza; there were two cases of each:
folliculitis, otitis externa, upper respiratory tract infection, pustule; for all other infections, there was one case of each (see footnote to Supplementary Table S8).
Symptoms observed in two patients receiving spesolimab were reported as drug reactions with eosinophilia and systemic symptoms (DRESS) with RegiSCAR scores of 1 and 3.33 Further details of these two case reports can be found in the Supplementary information below. Anti-drug antibodies (ADAs) were detected with a median time of 2.3 weeks after spesolimab administration. ADAs were detected in 23/50 (46%) patients who received at least one dose of spesolimab. The majority (87%) of the ADA-positive patients (40% of total treated) were also Nab positive and the NAb status appeared to be associated with the titer value. No dose-dependency observed; however, surprisingly the maximum ADA titers appear lowered in GPP
patients (n=4!) who received two doses by Day 8 vs 1 dose only.
DISCUSSION

[000193] This randomized trial of a single intravenous dose of the humanized anti-IL-36 receptor monoclonal antibody, spesolimab in patients with a flare of G PP, showed that at 1 week there was better clearance of lesions compared to placebo.
Overall, infections were more frequent with spesolimab, although there was no apparent pattern regarding pathogen and affected organs, and two patients treated with spesolimab had drug reactions with eosinophilia and systemic symptoms with RegiSCAR scores of 1 and 3.

[000194] At the end of the randomized 1 week period, a third of patients in the spesolimab group and most patients in the placebo group received open-label spesolimab and were followed for 12 weeks. As 15 of 18 randomized placebo patients received open-label spesolimab, the true effect of spesolimab versus placebo could not be reflected after week 1 in this trial. This highlights one of the inherent challenges of conducting a randomized placebo-controlled trial in patients with this disease; the episodic nature and severity of GPP flares (potentially life threatening) presented several challenges in the design of this study including limiting the duration as to how long patients could ethically be expected to undertake placebo treatment in this trial;
further, the clinical course is heterogeneous and can be characterized as a relapsing disease with recurrent flares, or a persistent disease with intermittent flares, making selection of appropriate timings for study endpoints difficult.

[000195] As noted above, this trial has limitations, including the small number of enrolled patients (typical for a rare disease); however, the effect size for the primary and key secondary endpoints at week 1 were large and significant. The randomized period was limited to 1 week and the option for patients, at week 1, to receive open-label treatment with spesolimab, if a pre-specified threshold for severity was still met, meant most patients on placebo received spesolimab, and thus comparative analyses after week 1 were non-informative; as such hierarchical tests of secondary endpoints defined at week 4, were not reported here. There were some baseline imbalances between the treatment arms (gender, race, and GPPASI) that occurred at random;

however, post hoc sensitivity analyses of the primary and key secondary endpoints adjusted for the imbalances were consistent with the primary analysis.

[000196] Despite these challenges, the rapid control of flare episodes in this study with a single infusion of spesolimab, builds on findings from a previous open-label study of spesolimab and supports the hypothesis that IL-36 is a central driver of the pathogenesis of GPP. Improvements over time in measures including CRP, neutrophil counts, pain, PSS, FACIT-Fatigue, DLQI are indicative that spesolimab acts systemically beyond the skin and suggests spesolimab has the potential to improve of the quality of life of patients experiencing a GPP flare. Chronic administration of spesolimab is being evaluated with a subcutaneous formulation in an ongoing 5-year open-label extension (ClinicalTrials.gov identifier: NCT03886246) and the prevention of flares trial Effisayil 2 (ClinicalTrials.gov identifier: NCT04399837).

[000197] In conclusion, in this phase 2 trial, GPP flares treated with intravenous spesolimab resulted in higher rates of clearance of pustular lesions compared with placebo but was associated with infections and systemic reactions. Longer and larger trials are required to determine the effect and safety of spesolimab in GPP.
Supplementary Information Generalized Pustular Psoriasis Physician Global Assessment (GPPGA)

[000198] GPPGA relies on the clinical assessment of the patient's skin presentation.
It is a modified Physician Global Assessment (PGA), a physician's assessment of psoriatic lesions, which has been adapted to the evaluation of patients with generalized pustular psoriasis (GPP). The investigator (or qualified site personnel) scores the erythema, pustules and scaling of all psoriatic lesions from 0 to 4 (see table below). Each component is graded separately, the average is calculated and the final GPPGA is determined from this composite score (composite mean score =
(erythema + pustules + scaling)/3; a total GPPGA score of 0 mean = 0 for all three components, a score of 1 means 0 to <1.5, score of 2 means 1.5 to <2.5, score of 3 means 2.5 to <3.5, and a score of 4 mean 3.5). A lower score indicates a lesser severity, with 0 relating to 'clear' and 1 relating to 'almost clear'. To receive a score of 0 or 1, the patient should be afebrile in addition to the skin presentation requirements.

Psoriasis Area and Severity Index for Generalized Pustular Psoriasis (GPPASI)

[000199] The GPPASI is an adaptation of the PASI, an established measure of severity and area of psoriatic lesions in patients with psoriasis, for patients with GPP.
Similar adaptions have been used for palmoplantar psoriasis. In the GPPASI, the induration component has been substituted by a pustules component. It is a tool that provides a numeric scoring for the patient's overall generalized pustular psoriasis disease state, ranging from 0 to 72. It is a linear combination of the percent of skin surface area (body region area score) that is affected and the severity (scored on a five-point scale, ranging from 0 [least severe] to 4 [most severe]) of erythema, pustules, and scaling (desquamation) over four body regions (head, upper limb, trunk, and lower limb)*.
Score Erythema Pustules Scaling 0 Normal or post- No visible pustules No scaling or crusting (clear) inflammatory hyperpigmentation 1 Faint, diffuse pink or Low density occasional Superficial focal scaling (almost slight red small discrete pustules or crusting restricted to clear) (noncoalescent) periphery of lesions 2 Light red Moderate density Predominantly fine (mild) grouped discrete small scaling or crusting pustules (noncoalescent) 3 Bright red High density pustules Moderate scaling or (moderate) with some coalescence crusting covering most or all lesions 4 Deep fiery red Very high-density Severe scaling or (severe) pustules with pustular crusting covering most lakes or all lesions *Individual score per body region = body region factor (head = 0.1, upper limb = 0.2, trunk = 0.3, lower limb = 0.4) x body region area score x sum of component severity scores in body region.
Total GPPASI score = sum of individual score from all body regions.
Inclusion and exclusion criteria Inclusion criteria

[000200] Patients will be enrolled (screened) into the trial, if they meet the following criteria:
la. Patients with a GPPGA score of 0 or 1 and a known and documented history of GPP (per ERASPEN criteria) regardless of IL36RN mutation status, and in addition with previous evidence of fever, and/or asthenia, and/or myalgia, and/or elevated C-reactive protein, and/or leukocytosis with peripheral blood neutrophilia (above the upper limit of normal [ULN]), OR
lb. Patients with a flare of moderate-to-severe intensity meeting the ERASPEN
criteria of GPP, with a known and documented history of GPP (per ERASPEN
criteria) regardless of IL36RN mutation status, and in addition with previous evidence of fever, and/or asthenia, and/or myalgia, and/or elevated C-reactive protein, and/or leukocytosis with peripheral blood neutrophilia (above ULN), OR
lc. Patients experiencing their first episode of a GPP flare of moderate-to-severe intensity with evidence of fever, and/or asthenia, and/or myalgia, and/or elevated C-reactive protein, and/or leukocytosis with peripheral blood neutrophilia (above ULN).
For these patients, the diagnosis will be confirmed retrospectively by a central external expert/committee.
2. Patients may or may not be receiving background treatment with retinoids and/or methotrexate and/or cyclosporine. Patients must discontinue retinoids/methotrexate/cyclosporine prior to receiving the first dose of spesolimab or placebo.
3. Male or female patients, aged 18-75 years at screening.
4. Signed and dated written informed consent prior to admission to the trial in accordance with ICH-GCP and local legislation prior to start of any screening procedures.
5. Women of childbearing potential must be ready and able to use highly effective methods of birth control per ICH M3 (R2) that result in a low failure rate of less than 1% per year when used consistently and correctly. A woman is considered of childbearing potential (WOCBP), i.e. fertile, following menarche and until becoming postmenopausal unless permanently sterile. Permanent sterilization methods include hysterectomy, bilateral salpingectomy and bilateral oophorectomy. Tubal ligation is not a method of permanent sterilization. A postmenopausal state is defined as no menses for 12 months without an alternative medical cause.
Exclusion criteria

[000201] Patients will not be screened or treated if any of the following criteria apply:
1. Patients with synovitis¨acne¨pustulosis¨hyperostosis¨osteitis syndrome.
2. Patients with primary erythrodermic psoriasis vulgaris.
3. Patients with primary plaque psoriasis vulgaris without presence of pustules or with pustules that are restricted to psoriatic plaques.
4. Drug-triggered acute generalized exanthematous pustulosis.
5. Immediate life-threatening flare of GPP or requiring intensive care treatment, according to the investigator's judgement. Life-threatening complications mainly include, but are not limited to, cardiovascular/cytokine-driven shock, pulmonary distress syndrome or renal failure.
6. Severe, progressive or uncontrolled hepatic disease, defined as >3-fold ULN

elevation in aspartate transaminase or alanine transaminase or alkaline phosphatase, or >2-fold ULN elevation in total bilirubin.
7. Treatment with:
a. Any restricted medication, or any drug considered likely to interfere with the safe conduct of the trial, as assessed by the investigator.
b. Any prior exposure to spesolimab or another IL36R inhibitor.
8. Patients with dose escalation of their maintenance therapy with cyclosporine and/or methotrexate and/or retinoids within the 2 weeks prior to receiving the first dose of spesolimab/ placebo.

9. The initiation of systemic agents such as cyclosporine and/or retinoids and/or methotrexate 2 weeks prior to receiving the first dose of spesolimab/placebo.
10. Patients with congestive heart disease, as assessed by the investigator.
11. Active systemic infections (fungal and bacterial disease) during the last 2 weeks prior to receiving first drug administration, as assessed by the investigator.
12. Increased risk of infectious complications (e.g. recent pyogenic infection, any congenital or acquired immunodeficiency [e.g. human immunodeficiency virus (HIV)], past organ or stem cell transplantation), as assessed by the investigator.
13. Relevant chronic or acute infections including HIV or viral hepatitis. For patients screened while having a flare (inclusion criteria lb or 1c), if Visit 1 HIV or viral hepatitis results are not available in time for randomization, these patients may receive randomized treatment as long as the investigator has ruled out active disease based on available documented history (i.e. negative HIV and viral hepatitis test results) within 3 months prior to Visit 2. A patient can be re-screened if the patient was treated and is cured from acute infection.
14. Active or latent tuberculosis (TB):
QuantiFERON (or if applicable, T-Spot ) TB test will be performed at screening. If the result is positive, the patient may participate in the trial if further work up (according to local practice/guidelines) establishes conclusively that the patient has no evidence of active tuberculosis. Patients with active TB must be excluded. If presence of latent tuberculosis is established, then treatment should have been initiated and maintained according to local country guidelines. For patients screened while having a flare (inclusion criteria lb or 1c), if the TB test results are not available in time for randomization, these patients may receive randomized treatment (provided they meet all other inclusion/exclusion criteria) as long as the investigator has ruled out active disease based on available documented history (i.e. negative for active TB) within 3 months prior to Visit 2.

15. History of allergy/hypersensitivity to a systemically administered trial medication agent or its excipients.
16. Any documented active or suspected malignancy or history of malignancy within years prior to screening, except appropriately treated basal or squamous cell carcinoma of the skin or in situ carcinoma of uterine cervix.
17. Currently enrolled in another investigational device or drug trial, or less than 30 days since ending another investigational device or drug trial(s) or receiving other investigational treatment(s).
18. Women who are pregnant, nursing or who plan to become pregnant while in the trial. Women who stop nursing before the trial drug administration do not need to be excluded from participating; they should refrain from breastfeeding up to 16 weeks after the trial drug administration.
19. Major surgery (major according to the investigator's assessment) performed within 12 weeks prior to receiving the first dose of trial drug or planned during the trial, e.g.
hip replacement, aneurysm removal, stomach ligation, as assessed by the investigator.
20. Evidence of a current or previous disease, medical condition (including chronic alcohol or drug abuse or any condition) other than GPP, surgical procedure, psychiatric or social problems, medical examination finding (including vital signs and electrocardiogram) or laboratory value at the screening outside the reference range that in the opinion of the investigator is clinically significant and would make the trial participant unreliable to adhere to the protocol, comply with all trial visits/procedures or to complete the trial, compromise the safety of the patient or compromise the quality of the data.

Supplementary Table S3. Efficacy Outcomes and Description Outcome Timepoint(s) Description measure Primary outcome GPPGA Week 1 No visible pustules pustulation subscore of 0 Key secondary outcome GPPGA score of 0 Week 1 Composite mean score = (erythema +
pustules +
or 1 scaling)/3; total GPPGA score given is 0 if mean is 0, and 1 if mean 0 to <1.5 for all three components:
Erythema, 0 (normal or post-inflammatory hyperpigmentation) to 4 (deep fiery red) Pustules, 0 (no visible pustules) to 4 (very high-density pustules with pustular lakes) Scaling, 0 (no scaling or crusting) to 4 (severe scaling or crusting covering most or all lesions) Secondary endpoints GPPASI 75 Week 4 75% improvement in GPPASI total score.
Composite mean score = sum of individual score (as defined per GPPGA) from all body regions. Individual score per body region = body region factor (head = 0.1, upper limb = 0.2, trunk = 0.3, lower limb = 0.4) x body region area score x sum of component severity scores in body region. Patients' overall GPPASI ranges from 0 to 72 Change from Week 4 PRO providing a range of scores from 0 to 100 in a baseline in pain continuous visual scale of 100 mm in length to indicate VAS score the severity of GPP pain during the previous week.
A
higher score indicates greater pain intensity Change from Week 4 PRO providing a range of 0 (none) to 4 (very severe) to baseline in PSS assess severity of pain, redness, itching, and burning score symptoms during the past 24 hours. The symptom scores are added to an unweighted total score, ranging from 0 to 16 Change from Week 4 PRO consisting of a 13-item questionnaire that assesses baseline in FACIT- self-reported fatigue and its impact upon daily activities Fatigue score and function during the previous week (7 days).
Responses of "not at all", "a little", "somewhat", "quite a bit", and "very much" are available for each question, and correspond to scores of 0, 1, 2, 3 and 4, respectively.
The total score ranges from 0 to 52. Items are reversed to provide a score in which lower scores indicate greater fatigue.
GPPGA score of 0 Week 4 or 1 GPPGA Week 4 pustulation subscore of 0 GPPASI 50 Weeks 1 and 4 50% improvement in GPPASI total score CGI, Clinical Global Impression; DLQI, Dermatology Life Quality Index; EQ-5D-5L, 5-level EuroQo1-5 dimensions; FACIT, Functional Assessment of Chronic Illness Therapy; GPP, generalized pustular psoriasis; GPPASI, Generalized Pustular Psoriasis Area and Severity Index;
GPPGA, Generalized Pustular Psoriasis Physician Global Assessment; IV, intravenous; JDA, Japanese Dermatological Association; OL, open-label; PRO, patient-reported outcome; PSS, Psoriasis Symptom Scale; VAS, visual analog scale.
Supplementary Table S4. Absence or Presence of Potential Pathogenic Variations of CARD14 and AP1S3 Mutations Based on Genotyping Spesolimab Placebo Tot (N=35) (N=18) (N=5 CARD14 mutationt*
Yes ¨ no. (%) 5 (14.3) 0 (0.0) 5 (9.
No ¨ no. (%) 24 (68.6) 14 (77.8) 38 (71 DNA sequencing not available ¨ no. (%) 6 (17.1) 4 (22.2) 10 (1E
AP1S3 mutationt Yes ¨ no. (%) 1 (2.9) 0 (0.0) 1 (1.
No ¨ no. (%) 28 (80.0) 14 (77.8) 42 DNA sequencing not available ¨ no. (%) 6 (17.1) 4 (22.2) 10 (1E
At the date of database lock (18 January 2021), DNA sequencing (targeted re-sequencing IIlumina Miseq, IIlumina Inc., San Diego, CA) was not yet completed in three patients and there were missing samples from seven patients. *Positive patients had heterozygous mutations (two p.Ser200Asn; one p.Arg682Trp; one p.Pro506Leu; one p.Ser539Arg). One patient in the spesolimab arm carried double CARD14 and IL36RN mutations (p.Ser200Asn and p.Ser113Leu, respectively).tThe single positive patient had a heterozygous mutation (p.Phe4Cys). One patient in the spesolimab arm carried double AP1S3 and IL36RN mutations (p.Phe4Cys and p.Leu27Pro, respectively).
Supplementary Table S5. Post-hoc Sensitivity Analysis for the Primary Endpoint with Linear Regression Adjusting for Gender, Race, and Baseline GPPASI

Spesolimab Placebo Spesolimab Placeb Gender Female Male Responders/total patients 11/21 1/15 8/14 0/3 Adjusted risk difference (95% CI)* 0.482 (0.269, 0.695) P-value for treatment t <0.001 Race Asian White Responders/total patients 10/16 1/13 9/19 0/5 Adjusted risk difference (95% Cl)* 0.522 (0.314, 0.729) P-value for treatment t <0.001 Baseline GPPASI value Adjusted risk difference (95% Cl)* 0.469 (0.254, 0.683) P-value for treatment t <0.001 *Based on a linear regression model using the primary endpoint as the dependent variable, and treatment assignment plus the respective covariate as independent variable. 95% Cl and P-value based on robust SE. tP-values for treatment difference are based on a linear regression model.
Cl, confidence interval;
GPPSI, Psoriasis Area and Severity Index for Generalized Pustular Psoriasis;
SE, standard error.
Supplementary Table S6. Post-hoc Sensitivity Analysis for the Key Secondary Endpoint with Linear Regression Adjusting for Gender, Race, and Baseline GPPASI
Spesolimab Placebo Spesolimab Placeb Gender Female Male Responders/total patients 10/21 2/15 5/14 0/3 Adjusted risk difference (95% Cl)* 0.346 (0.113, 0.579) P-value for treatment t 0.005 Race Asian White Responders/total patients 8/16 2/13 7/19 0/5 Adjusted risk difference (95% Cl)* 0.354 (0.125, 0.583) P-value for treatment t 0.004 Baseline GPPASI value Adjusted risk difference (95% Cl)* 0.297 (0.058, 0.535) P-value for treatment t 0.018 *Based on a linear regression model using the primary endpoint as the dependent variable, and treatment assignment plus the respective covariate as independent variable. 95% Cl and P-value based on robust SE. tP-values for treatment difference are based on a linear regression model.
Cl, confidence interval;
GPPSI, Psoriasis Area and Severity Index for Generalized Pustular Psoriasis;
SE, standard error.

Exploratory Efficacy Endpoints Post day 8 by Randomized Treatment at Day 1 and Open Label Spesolimab Treatment at Day 8. Descriptive analyses for GPPGA
pustulation subscore of 0 and GPPGA total score of 0 or 1 by four groups:

[000202] In patients, originally randomized to spesolimab, who received spesolimab on day 1 with or without open-label spesolimab on day 8 (N=35), 19 patients (54%) achieved a GPPGA pustulation subscore of 0 and 15 patients (43%) achieved a GPPGA total score of 0 or 1 at the end of week 1; at week 12, 21 patients (60%) had a GPPGA pustulation subscores of 0 and GPPGA total scores of 0 or 1 (Figures 5 and 6).Of the 23 patients randomized to spesolimab who did not receive open-label spesolimab on day 8, 19 (83%) achieved a GPPGA pustulation subscore of 0 and (65%) achieved a GPPGA total score of 0 or 1 at the end of week 1; at week 12, these scores were reported in 15 (65%) and 16 (70%) patients, respectively (Figures and 6). In patients, originally randomized to spesolimab, who received open-label spesolimab on day 8 (N=12), five patients (42%) achieved a GPPGA pustulation subscore of 0 and two patients (17%) achieved a GPPGA total score of 0 or 1 at the end of week 2; at week 12, six patients (50%) and seven patients (58%) had GPPGA pustulation subscores of 0 and GPPGA total scores of 0 or 1 (Figures 5 and 6). Of the 15 patients in the placebo group who received open-label spesolimab on day 8, 11(73%) achieved a GPPGA pustulation subscore of 0 and eight (53%) achieved a GPPGA total score of 0 or 1 at the end of week 2 (seven days post-open-label spesolimab); at week 12, these scores were reported in six (40%) and eight (53%) patients, respectively (Figures 5 and 6).
Descriptive analyses for the planned hierarchical secondary endpoints GPPASI
75 and change from baseline in: pain VAS; PSS; and FACIT-Fatigue at week 4 by four groups

[000203] For GPPASI 75 at week 4, in patients, originally randomized to spesolimab, who received spesolimab on day 1 with or without open-label spesolimab on day 8, 18/35 (51%) achieved a GPPASI 75. Of the 23 patients randomized to spesolimab who did not receive open-label spesolimab on day 8, (70%) achieved a GPPASI 75 at week 4 (Supplementary Table S7). Of the 12 patients in the spesolimab group and 15 patients in the placebo group who received an open-label dose of spesolimab at the end of 1 week, two (17%) and six (40%) patients, respectively, achieved a GPPASI 75 at week 4 (Supplementary Table S7).

[000204] For pain VAS at week 4, in patients, originally randomized to spesolimab, who received spesolimab on day 1 with or without open-label spesolimab on day 8, there was a median (interquartile range; IQR) change from baseline of -53.4 (-77.8, -20.2) (Supplementary Table S7). Of the 23 patients randomized to spesolimab who did not receive open-label spesolimab on day 8, there was a median (IQR) change from baseline of -63.1 (-79.8, -22.5). Of the 12 patients in the spesolimab group and 15 patients in the placebo group who received an open-label dose of spesolimab there were median (IQR) changes from baseline of -44.6 (-71.2, -17.3) and -54.3 (-79.0, 33.3), respectively (Supplementary Table S7).

[000205] For PSS at week 4, in patients, originally randomized to spesolimab, who received spesolimab on day 1 with or without open-label spesolimab on day 8, there was a median (IQR) change from baseline of -7.0 (-10.0, -3.0) (Supplementary Table S7). Of the 23 patients randomized to spesolimab who did not receive open-label spesolimab on day 8, there was a median (IQR) change from baseline of -7.0 (-11.0, -2.0). Of the 12 patients in the spesolimab group and 15 patients in the placebo group who received an open-label dose of spesolimab there were median (IQR) changes from baseline of -6.0 (-7.0, -3.5) and -5.0 (-9.0, -2.0), respectively (Supplementary Table S7).

[000206] For FACIT-Fatigue at week 4, in patients originally randomized to spesolimab, who received spesolimab on day 1 with or without open-label spesolimab on day 8, there was a median (IQR) change from baseline of 22.0 (1.0, 31.0) (Supplementary Table S7). Of the 23 patients randomized to spesolimab who did not receive open-label spesolimab on day 8, there was a median (IQR) change from baseline of 22.0 (3.0, 35.0) (Supplementary Table S7). Of the 12 patients in the spesolimab group and 15 patients in the placebo group who received an open-label dose of spesolimab there were median (IQR) changes from baseline of 15.0 (5.5, 28.5) and 16.0 (-19.0, 26.0), respectively (Supplementary Table S7).
Supplementary Table S7. GPPASI 75, Pain VAS, PSS, and FACIT-Fatigue at Week 1 and 4 by Randomized Treatment at Day 1 and Open-Label Spesolimab Treatment at Day 8.

GPPASI 75 Baseline (day 1) Week 1 (day 8) Week 4 Proportion of patients - no. (%) (95% Cl) Spesolimab randomized all (N=35) 4 (11.4) 18 (51.4) (4.5, 26.0) (35.6, 67.0) Spesolimab randomized 900 mg single 4 (17.4) 16 (69.6) dose only (N=23) (7.0, 37.1) (49.1, 84.4) Spesolimab randomized 900 mg single 0 (0.0) 2 (16.7) dose + open label day 8 spesolimab (0.0, 24.2) (4.7,44.8) (N=12) Placebo randomized + open label day 8 0 (0.0) 6 (40.0) spesolimab (N=15) (0.0, 20.4) (19.8, 64.3) Baseline (day 1) Week 1 (day 8) Week 4 Median absolute Median absolute Median (IQR) change from change from Pain VAS baseline (IQR) _________________________________ baseline (IQR) Spesolimab randomized all (N=35) 79.8 -21.3 -53.4 (20, 100) (-55.3, -3.1) (-77.9, -20.2) Spesolimab randomized 900 mg single 79.8 -27.6 -63.1 dose only (N=23) (62.8, 87.8) (-56.3, -9.6) (-79.8, -22.5) Spesolimab randomized 900 mg single 78.7 -9.2 -44.6 dose + open label day 8 spesolimab (75.3, 86.0) (-36.5, -1.0) (-71.2, -17.3) (N=12) Placebo randomized + open label day 8 71.9 -18.8 -54.3 spesolimab (N=15) (50.0, 89.4) (-38.8, 18.0) (-79.0, 33.3) Baseline (day 1) Week 1 (day 8) Week 4 Median (IQR) Median absolute Median absolute change from change from P55 baseline (IQR) baseline (IQR) Spesolimab randomized all (N=35) 11.0 -4.0 -7.0 (3.0, 16.0) (-7.0, 0.0) (-10.0, -3.0) Spesolimab randomized 900 mg single 11.0 -5.0 -7.0 dose only (N=23) (9.0, 12.0) (-8.0, -1.0) (-11.0, -2.0) Spesolimab randomized 900 mg single 10.5 0.0 -6.0 dose + open label day 8 spesolimab (9.0, 13.5) (-3.5, 2.5) (-7.0,-3.5) (N=12) Placebo randomized + open label day 8 10.0 -1.0 -5.0 spesolimab (N=15) (9.0, 11.0) (-5.0, 2.0) (-9.0, -2.0) Baseline (day 1) Week 1 (day 8) Week 4 Median (IQR) Median absolute Median absolute changefrom change from FACIT-Fatigue baseline (IQR) baseline (IQR) Spesolimab randomized all (N=35) 14.0 7.0 22.0 (1.0, 49.0) (1.0, 20.0) (1.0, 31.0) Spesolimab randomized 900 mg single 13.0 12.0 22.0 dose only (N=23) (6.0, 31.0) (2.0, 20.0) (3.0, 35.0) Spesolimab randomized 900 mg single 17.0 4.5 15.0 dose + open label day 8 spesolimab (10.0, 25.5) (0.5, 16.5) (5.5, 28.5) (N=12) Placebo randomized + open label day 8 17.0 3.0 16.0 spesolimab (N=15) (6.0, 33.0) (-3.0, 11.0) (-19.0, 26.0) This exploratory dataset includes the analyses originally planned for hierarchical testing of secondary endpoints (GPPASI 75 and change from baseline in: pain VAS; PSS; and FACIT-Fatigue) all at week 4, in the statistical analysis plan; however, randomization to trial groups no longer pertained after week 1 as 15 of 18 patients assigned to placebo received open-label spesolimab on day 8.
For this analysis, any values post open-label spesolimab at day 8 are used, but any values post use of escape medication, or rescue medication with spesolimab are not used and imputed as the worst outcome in the calculation of median and quartiles. CI, confidence interval; GPPASI 75, 75% or greater improvement in the Psoriasis Area and Severity Index for Generalized Pustular Psoriasis; FACIT-Fatigue, Functional Assessment of Chronic Illness Therapy-Fatigue; IQR, interquartile range; Pain VAS, pain visual analog scale; PSS, Psoriasis Symptom Scale.
Descriptive analyses for GPPASI, GPPASI 75, pain VAS, DLQI, neutrophil counts and C-reactive protein levels by the group of patients randomized to spesolimab who received one (day 1) or two doses (day 1 plus day 8); N=35) of spesolimab:

[000207] The median percent improvement in the GPPASI score from baseline was 43% at week 1 and progressively increased up to 82% at week 12 (Figure 7A). A
GPPASI 75 was achieved in 13 patients (37%) at week 2, in 18 patients (51%) at week 4, 21 patients (60%) at week 8 and 20 patients (57%) at week 12 (Figure 7B).
Rapid reductions in pain VAS score and improvements in DLQI scores were achieved through week 4 and through week 12 (Figure 7C and 7D). In patients, with elevated baseline neutrophils, counts were normalized within 1 week of receiving spesolimab (Figure 7E). Median C-reactive protein (CRP) normalized within 2 weeks in patients with elevated baseline CRP 10mg/L), who received one or two doses of spesolimab (Figure 7F).
Supplementary Table S8. Summary of Adverse Events by System Organ Class n (%) Week 1 Week 12*
[rate/100 patient-years]
Spesolimab Placebo Spesolimab (N=35) (N=18) (N=51) Adverse events by System Organ Class*
Skin and subcutaneous tissue 5 (14.3) 2 (11.1) 14 (27.5) disorders [822.6] [593.9]
[137.9]
6 (17.1) 1 (5.6) 24 (47.1) Infections and infestations*
[987.2] [292.2]
[262.8]
General disorders and 9 (25.7) 5 (27.8) 13 (25.5) administration site conditions [1543.3] [1756.0]
[127.5]
4 (11.4) 2 (11.1) 7 (13.7) Investigations [664.1] [619.1] [61.5]

Blood and lymphatic system 1 (2.9) 2 (11.1) 4 (7.8) disorders [154.1] [640.8] [32.9]

Metabolism and nutrition 3 (8.6) 2 (11.1) 4 (7.8) disorder [484.8] [624.4] [32.4]

4 (11.4) 3 (16.7) 7 (13.7) Nervous system disorders [655.2] [961.2] [60.5]

Musculoskeletal and 4 (11.4) 2 (11.1) 11 (21.6) connective tissue [627.0] [624.4]
[101.6]
2 (5.7) 1 (5.6) 13 (25.5) Gastrointestinal disorder [309.5] [292.2]
[118.5]
All AEs occurring between start of treatment and end of the residual effect period (16 weeks after the last dose of trial) were considered 'treatment-emergent'. AEs were coded using the Medical Dictionary for Drug Regulatory Activities version 23.1. The AE severity was graded according with the Rheumatology Common Toxicity Criteria version 2.0 safety analysis set.
Pustular psoriasis was excluded as an adverse event from this safety analysis.*Dataset at week 12 includes patients randomized to spesolimab who received up to three doses of spesolimab, and patients initially randomized to placebo who received open-label spesolimab at day 8. All adverse events from the first use of spesolimab to the residual effect period of the last use of spesolimab are included. tAdverse AEs by System Organ Class are reported in 10`)/0 of patients in any treatment group.
Example 2 Spesolimab treatment improves pain, symptoms of psoriasis, fatigue and quality of life in patients with generalized pustular psoriasis: Patient-reported outcomes results from the Effisayil 1 study

[000208] Introduction & Objectives: Generalized Pustular Psoriasis (GPP) is a rare and potentially life-threatening skin disease, characterised by widespread sterile pustules and recurrent flares. Signs and symptoms associated with GPP include pain, fever and fatigue, which can impact activities of daily living and overall quality of life (QoL). Spesolimab has been reported to rapidly improve pustular and skin clearance in patients presenting with a GPP flare. The objective of this analysis was to evaluate patient reported outcomes (PROs) on measures of pain, symptoms of psoriasis, fatigue and impact on overall QoL in patients treated with spesolimab from the Effisayil 1 study.

[000209] Materials & Methods: Effisayil 1 (NCT03782792) was a multicentre, randomised, double-blind, placebo-controlled study in patients with a GPP
flare.
Eligible patients (n=53) were randomly assigned (2:1) to receive a single intravenous (IV) dose of open-label 900 mg spesolimab or placebo on Day 1, followed for 12 weeks. At Week 1, patients were eligible to receive an open-label, single IV
dose of 900 mg spesolimab if they had a GPP Physician Global Assessment (GPPGA) score and did not receive escape medication before day 8. The secondary and further endpoints were assessed by the Psoriasis Symptom Scale (PSS; 0-16, with higher scores indicating more severe symptoms), pain Visual Analogue Scale (pain VAS;

[no pain] to 100 [severe pain]), Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-Fatigue; 0-52, with lower scores indicating greater impact) and the Dermatology Life Quality Index (DLQI; 0 [no effect] to 30 [extremely large effect]) over time through to the end of the study. Patients who received escape treatment (an optional standard of care treatment administered to patients within 1 week of their first dose) or rescue medication (an optional 900mg IV dose of spesolimab administered anytime from Day 8 onwards) are considered as non-responders for later timepoints in analysis.

[000210] Results: Patients were randomised to receive 900 mg spesolimab (n=35) or placebo (n=18). At baseline, patients had a high clinical burden and impaired QoL as indicated by median PSS (11.0), pain VAS (79.8), FACIT-Fatigue (14.0) and DLQI

(19.5) scores (Table 9). Improvements in PSS, Pain VAS, FACIT-Fatigue and DLQI

scores were observed from baseline to Week 12 (Table). Pain improved as early as Week 1, with further improvements at Week 4 that were maintained to Week 12.
For the other PROs (PSS, FACTI-Fatigue and DLO!), improvements were also seen as early as Week 1 but continued to improve through to Week 12.

[000211] .. Conclusions: Patients receiving 900 mg spesolimab IV and an optional second dose at day 8 showed marked improvements in PROs of pain, symptoms of psoriasis, fatigue and overall QoL. These improvements were seen early and were maintained for up to 12 weeks. These findings suggest that up to 2 doses of spesolimab can result in rapid pustular clearance of GPP flares which were associated with improvements in patient-reported outcomes.
Table 9. Baseline and absolute change from baseline in PROs of the spesolimab arm (n=35) .................................................... _____________________ ................................ õõ..õ.õõ..õ.õõ.õ.õõ.õõõõ.õõõõ.õõ..õ.õõ.õ.õõõ
..
Patient-Reportei Outcomes Madt.art L. ...........
(KIR) PSS Pain-VAS iFACIT-latigue DLQ1 Baseline i 114 (9,0-12.0) 79.3 (70.5-87.8) 14..0 (LiDl---28õ0) 19.$ (1.6.a-2sm I Week I t -4.0 (-7,0, 0:0) -213 (-553, -3.1) 7,01:14, 20,0) ......................................................................... ----t 'Week 4 -7J0 (40,0, -3..0) -53,4 (-77,9, -20,2) 20,0 OA. 34,0) 93 (45,0, I Wk 1.2: -5 (-lag NR) -52.6(17,9, NR) I 24..0 (UR, 32r0) 415 (-ISA ID) All datasets include patients randomized to spesolimab who received up to two doses of spesolimab, including 12 patients who received an open-label, single, intravenous dose of 900 mg spesolimab (second dose) at week 1.
Any values post open label spesolimab at week 1 are used, but any use of escape medication, or rescue mediation with spesolimab represents non-response.
IQR, Interquartile range.
Example 3 Spesolimab treatment improves pain, symptoms of psoriasis, fatigue and quality of life in patients with generalized pustular psoriasis: Patient-reported outcomes from the Effisayil 1 study

[000212] PURPOSE: To evaluate PROs on measures of pain, symptoms of psoriasis, fatigue and impact on overall quality of life (QoL) in patients treated with spesolimab in the EffisayilTM 1 study.

SUBSTITUTE SHEET (RULE 26)

[000213] INTRODUCTION: GPP is a rare, potentially life-threatening, neutrophilic skin disease characterised by widespread eruption of sterile, visible pustules, and can occur with or without systemic inflammation. In the multicentre, randomised, double-blind, placebo-controlled EffisayilTM 1 study (NCT03782792) in patients presenting with a GPP flare, spesolimab treatment led to rapid pustular and skin clearance within 1 week. GPP flares are associated with a high clinical burden in PROs including symptoms such as pain, itching and fatigue, which all impact the patient's overall QoL.
Here we explore PROs in patients with a GPP flare receiving spesolimab treatment.

[000214] METHODS: Patients (N=53) were randomised (2:1) to receive placebo or 900 mg of spesolimab on Day 1 and were followed for 12 weeks. Patients were able to receive standard of care (SoC) treatment any time after receiving their first dose of spesolimab or placebo on Day 1 and before Day 8. Patients who did not receive SoC
treatment during Week 1 were eligible to receive open-label (OL) spesolimab on Day 8 and one rescue dose of OL spesolimab between Day 8 and Week 12.

[000215] Spesolimab arm (n=35): One dose of spesolimab at Day 1 (n=23);
optional second dose of OL spesolimab at Day 8 (n=12); optional third dose of OL
spesolimab between Weeks 1 and 12 (n=2).

[000216] Placebo arm (n=18): optional first dose of OL spesolimab at Day 8 (n=15);
optional second dose of OL spesolimab between Weeks 1 and 12 (n=1).

[000217] Patients who already received their one rescue dose of spesolimab were treated with escape treatment (SoC) for any subsequent flares. All randomised patients were included in this analysis. The observed cases irrespective of any use of escape treatment, OL spesolimab on Day 8 or rescue spesolimab treatment after Day 8 (representing the intention-to-treat principle) are summarised descriptively. To monitor any changes in outcomes, patients completed the following PRO
questionnaires throughout the study: Psoriasis Symptom Scale [PSS], pain visual analogue scale (pain VAS), Functional Assessment of Chronic Illness Therapy¨
Fatigue (FACIT-Fatigue) and the Dermatology Life Quality Index (DLQI; Figure 10).

All four PROs were measured on Day 1, Day 8 and Weeks 2-4, 8 and 12. PSS
scores were also measured every day during Week 1 (data for days 4-7 not shown).

[000218] CONCLUSION: In this study, patients who received 900 mg of intravenous spesolimab showed clinically significant improvements from baseline in the PROs of pain, symptoms of psoriasis, fatigue and overall quality of life. The clear separation of the spesolimab and placebo curve occurred early during the placebo-controlled period (Week 1) (Figure 11). This suggests that spesolimab results in the rapid improvement of PROs with considerable improvement in fatigue and pain. PRO scores continued to improve up to Week 4 and were sustained through to Week 12 (Figure 11).
Example 4 Sustained treatment effect of spesolimab over 12 weeks for generalized pustular psoriasis flares; results from the Effisayil-1 study

[000219] The objective of this study was to analyze the effects of spesolimab over 12 weeks for treatment of patients presenting with a flare of generalized pustular psoriasis.

[000220] In EffisayilTM 1, a double-blind, randomized, placebo-controlled study in patients presenting with a flare of generalized pustular psoriasis (GPP), a rare, life-threatening autoinflammatory disease, spesolimab, an anti-IL-36 receptor antibody, led to rapid clearance (within 1-week) of pustular and skin lesions. Here, we explore the effects of spesolimab over the 12-week duration of the study (intention-to-treat analysis).

[000221] Patients (n=53) were randomized to receive a single intravenous dose of 900 mg spesolimab (n=35) or placebo (n=18) on Day 1. At Day 8, patients were eligible to receive an open-label, dose of spesolimab if they presented persistent symptoms; comprising 12 in the spesolimab group (34.3%) and 15 (83.3%) placebo.
Of patients initially randomized to spesolimab who received up to two doses (n=35), 61.8% and 84.4% achieved a GPPGA pustulation subscore of 0, and 50.0% and 81.3% a GPPGA total score of 0/1 by Weeks 1 and 12, respectively. Of patients initially randomized to placebo that received open-label spesolimab at Day 8, 83.3% and 80.0% had a GPPGA pustulation subscore of 0 and 72.2% and 93.3% had a GPPGA
total score of 0/1 by Weeks 2 (1-week post-spesolimab) and 12, respectively.
After Day 8, 32 and 17 patients randomized to spesolimab and placebo respectively, completed the 12-week follow-up period, during which four and two patients, required rescue treatment with spesolimab for a new flare episode.

[000222] Spesolimab demonstrated rapid and sustained clinical improvements over 12 weeks. These data further support spesolimab as a potential therapeutic option for patients with a G PP flare.
Example 5 RESULTS OF CLINICAL IMMUNOGENICITY EVALUATION ¨ Trial 1368-0013:
Testing spesolimab (BI 655130) in Patients With a Flare-up of a Skin Disease Called Generalized Pustular Psoriasis

[000223] Analysis methods: Blood samples for assessment of ADA/NAb were collected from subjects (patients) before the initiation and during the course of spesolimab treatment, and at end of study/discontinuation. All samples were first analyzed in the ADA screening assay and only those found to be putative positive were subsequently assessed in the ADA confirmatory assay. Only samples that confirmed positive were titrated to obtain a titer value. In addition, development of NAbs was evaluated only in subjects with ADA-positive samples.

[000224] Evaluation of spesolimab immunogenicity was performed using data from all evaluable subjects, defined as subjects who had a baseline immunogenicity assessment and at least 1 post-baseline value. For spesolimab treated subjects, baseline samples were considered to be the last sample obtained before initiation of active treatment. For patients who were not treated with spesolimab, baseline samples were the last sample before receiving placebo treatment.

[000225] Trial background: This was a randomized, placebo-controlled, double-blind, parallel-group, single-dose trial with 2 treatment groups (900 mg i.v.
spesolimab and placebo on Day 1). Patients could qualify to receive an open-label (OL) treatment with 900 mg i.v. spesolimab on Day 8 and rescue treatment with spesolimab after Day 8, depending on their GPPGA total score and the GPPGA pustulation subscore.
Patients were offered to roll over into the open-label extension (OLE) trial 1368-0025 if they met the inclusion criteria of this OLE trial. The follow-up period of trial 1 368-001 3 was 12 to 28 weeks, depending on the timing of the last spesolimab dose in trial 0013 and on whether patients continued in the OLE trial.

[000226] A total of 53 male and female patients with GPP were randomized in a 2:1 ratio to receive a single dose of spesolimab 900 mg i.v. (35 patients) or placebo (18 patients) on Day 1. Spesolimab as open-label dose on Day 8 was administered to patients overall and as rescue medication after Day 8 to a total of 6 patients.

[000227] More female (N=36) than male (N=17) patients were randomized. All patients were Asian (N=29) or White (N=24). The mean (StD) age was 43.0 (10.9) years and the mean (StD) weight was 72.0 (24.7) kg.

[000228] PK and ADA/NAb samples were collected predose, Day 4, Week 1, 2, 3, 4, 8, and end of study. For patients who did not enter the OLE 1368-0025 trial, the End-of-study Visit is 16 weeks after the last spesolimab dose. For patients who entered the OLE trial, the End-of-study Visit is Week 12, or 6 weeks after the last dose if patient received a rescue dose during Week 7-12.

[000229] Anti-drug antibody/ Neutralizing antibody (ADA/Nab) Responses: In trial 1368-0013, out of 53 randomized patients, 2 patients who were randomized to placebo on Day 1 did not receive any spesolimab treatment during the trial.
All ADA
samples from these 2 patients were negative. Of 51 spesolimab treated patients, 50 patients were ADA evaluable with available ADA assessments pre- and post-baseline, including 1 patient who received placebo on Day 1 and an OL rescue spesolimab after Day 8. All 50 ADA-evaluable patients were ADA-negative at baseline.

[000230] The incidence rate of ADA+ patients observed in 1368-0013 is comparable with that from the proof-of-concept trial 1368-0011. Overall, 23 (46%) of the evaluable and spesolimab-treated patients were ADA positive after treatment, and 27 patients (54%) were ADA negative through the trial duration. Twelve patients (24%) had a maximum titer of greater than 4000. The majority (87%) of the ADA-positive patients (40% of total treated) were also NAb positive and the NAb status appeared to be associated with the titer value. All ADA samples with titer value greater than 4000 were NAb positive. For ADA samples with titer value less than 4000, some were neutralizing, while others were not. The lower the titer values, the more likely the ADA
samples were NAb negative. In ADA-positive patients, ADA developed early with a median onset time of 2.3 weeks and reached maximum titer at a median time of 11.7 weeks. In approximately 75% of patients, maximum titer occurred at the last sample collected. The time to maximum ADA titer and the titer itself may be influenced by the duration of the trial and collection times. In NAb-positive patients, Nab was detected at a median onset time of 6.7 weeks. At the end of the trial (12 to 17 weeks after the first active dose), the ADA was resolved in 4 out of 23 ADA-positive patients.
Nineteen (38% of total ADA evaluable) patients remained ADA positive, 18 (36%) patients remained NAb positive, and 12 (24%) patients had a titer greater than 4000.

[000231] The ADA incidence rate was similar between patients treated with 1 dose of spesolimab and those who received 2 doses. However, the maximum ADA titers were observed to be lower in patients who received 2 doses of i.v. spesolimab within the first 8 days (Day 1 and Day 8) compared with patients who received only 1 i.v.
dose (Table 10, Figure 12).
Table 10. Trial 1368-0013: Incidence and kinetics of spesolimab ADAs with i.v.

spesolimab treatment Patients who received Patients who received Patients who received at least 1 dose of only 1 dose of 2 doses of spesolimab i.v. during spesolimab i.v. ..
spesolimab i.v.
the trial' (either on Day 1 (on both Day 1 or Day 8)2 and Day 8)2 Total, N ( /0) 50(100%) 37 (100%) 12(100%) Baseline3 ADA 0 (0%) 0 (0%) 0 (0%) positive, N ( /0) ADA negative'', N ( /0) 27 (54%) 20 (54%) 6 (50%) Treatment-induced 23 (46%) 17 (46%) 6 (50%) ADA positive5, N ( /0) Transient positive6, N 0 (0%) 0 (0%) 0 (0%) (94,) Potentially persistent 23 (46%) 17 (46%) 6 (50%) positive7, N ( /0) Persistent positive, N 0 (0%) 0 (0%) 0 (0%) (94,) Patients with 12 (24%) 10 (27%) 2 (17%) maximum titer >4000, N ( /0) Last sample ADA 19 (38%) 14 (38%) 5 (42%) positive, N ( /0) NAb positive9, N ( /0) 20 (40%) 14 (38%) 6 (50%) Last sample NAb 18 (36%) 13 (35%) 5 (42%) positive, N ( /0) ADA onset [week] 2.3 (1.0 to 11.7) 3.0 (1.0 to 11.7) 2.0 (1.7 to 4.0) (median, min to max) Time of maximum titer 11.7 (2.0 to 16.7) 11.6 (2.0 to 16.3) 12.9 (2.0 to 16.7) [week] (median, min to max) Maximum titer 7200 43200 3240 (median, min to max) (180 to 3 600 000) (180 to 3 600 000) (360 to 21 600) NAb onset [week] 6.7 (1.8 to 11.7) 7.3 (1.8 to 11.7) 3.6 (2 to 8.1) (median, min to max) 1 Also included a patient who did not receive any spesolimab on Days 1 or 8 but received a rescue treatment with spesolimab i.v. at Week 6.
2 Regardless of whether a patient received rescue treatment with spesolimab i.v. after Day 8 3 The last sample obtained before initiation of active spesolimab treatment 4'5'6'7'8'9 As defined in Section 5.2.1 of the BLA

[000232] In addition, females appeared to have higher immunogenicity response.
The ADA incidence rate was 58% and 24% in females and males, respectively. The percentage of patients with maximum titer of greater than 4000 was 30% in females vs. 12% in males (Table 11). Considering the small number of ADA positive male patients, there appear to be no significant differences in the maximum titers between genders.
Table 11. Trial 1368-0013: ADA incidence in female and male patients with GPP after i.v. administration of spesolimab Total Female Male N (spesolimab treated and 50 33 17 evaluable) ADA incidence 46% (23/50) 58% (19/33) 24%
(4/17) % of patient with maximum titer 24% (12/50) 30% (10/33) 12%
(2/17) >4000

[000233] Relationship of treatment-emergent ADA/NAb to efficacy

[000234] For the analysis, ADA titer groups were defined based on terciles of the maximal ADA titer across patients. The maximal ADA titer observed in the trial was used to distinguish 3 groups based on the maximum ADA titer ([33.3%
percentile, 1440], [>33.3% percentile and 66.6% percentile],and [>66.6% percentile, 43200]). In addition, the ADA vs. efficacy analysis differentiated between NAb-negative and NAb-positive patients. For the patients included in the NAb-negative group, all samples were NAb-negative or ADA-negative. The patients included in the NAb-positive group had at least 1 sample that was NAb-positive.

[000235] Generally, in trial 1368-0013, the proportion of patients with a GPPGA
pustulation subscore of 0 or a GPPGA total score of 0 or 1 over time was similar for ADA-negative and ADA-positive patients. When patients were grouped according to the terciles defined above, the response rates over time were generally similar across all 3 titer groups. Therefore, in general, this is also true when patients are categorized by having titer values below 4000 and above 4000. At Week 12, there appeared to be a drop in the response rate for patients with titer values greater than 43200.
From the 8 patients with titer values greater than 43200, 4 had a reduction in the respective score while the other 4 did not. The drop in response rate was less strong with respect to the score itself, because 3 of the 4 patients had an increase by 1 point.
Example 6 Spesolimab treatment improves CGI scores via the JDA severity index in patients with GPP

[000236] In the EffisayilTM 1 trial, patients with a generalized pustular psoriasis (G PP) flare achieved pustular and skin clearance after receiving spesolimab. We report change from baseline in JDA severity index clinical global impression (CG I) scores at Week 1. Patients (N=53) were randomly assigned (2:1) to receive intravenous spesolimab 900 mg or placebo on Day 1 and followed for 12 weeks. In the spesolimab arm (n=31), 67.7% of patients saw improvement (scores 1-3), and 32.3% saw no improvement (scores 4-5). In the placebo arm (n=15), 53.3% of patients saw improvement, and 46.7% saw no improvement. A score of 1 (very much improved) was achieved by 76.2% of spesolimab improvers. In conclusion, spesolimab treatment improved JDA CGI scores in patients with a GPP flare compared with placebo.

[000237] While certain aspects and embodiments of the invention have been described, these have been presented by way of example only, and are not intended to limit the scope of the invention. Indeed, the novel methods and systems described herein may be embodied in a variety of other forms without departing from the spirit thereof. The accompanying claims and their equivalents are intended to cover such forms or modifications as would fall within the scope and spirit of the invention.

[000238] All patents and/or publications including journal articles cited in this disclosure are expressly incorporated herein by reference.

Claims (30)

Claims

1. A method of treating generalized pustular psoriasis (GPP) flares in a patient, said method comprising administering to the patient two 900 mg intravenous (i.v.) doses of an anti-IL-36R antibody; wherein the second dose is administered less than weeks after the first dose.

2. A method of treating GPP in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

3. A method of reducing or alleviating signs and symptoms of an acute phase flare-up of GPP in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

4. A method of reducing the severity and duration of GPP symptoms, said method comprising including administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

5. A method of treating a skin disorder associated with GPP, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R
antibody; wherein the second dose is administered less than 2 weeks after the first dose.

6. A method of preventing the recurrence of GPP flares in a patient, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

7. A method of reducing pain by at least 10% in a patient with GPP, said method comprising administering to the patient two 900 mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

8. A method of improving the quality of life by at least 10% in a patient with moderate to severe GPP symptoms, said method comprising administering to the patient two mg intravenous doses of an anti-IL-36R antibody; wherein the second dose is administered less than 2 weeks after the first dose.

9. The method according to any of the preceding claims, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of 2.

10.The method according to any of the preceding claims, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2.

11.The method according to any of the preceding claims, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before the administration of the first i.v. dose.

12.The method according to any of the preceding claims, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 after the administration of the first i.v. dose.

13.The method according to any of the preceding claims, wherein the patient has a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA
pustulation subscore of 2 before and after the administration of the first i.v. dose.

14.The method according to any of the preceding claims, wherein the second dose is administered after 1 week but less than 2 weeks from the first dose.

15.A method of treating a GPP patient with a GPPGA pustulation subscore of 2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (I.V.) dose of an anti-IL-36R antibody; (b) assessing the GPPGA
pustulation subscore of the patient and if the GPPGA pustulation subscore of 2 of the patient persists after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

16.A method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of >=2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (i.v.) dose of an anti-IL-36R antibody;
(b) assessing the GPPGA total score of the patient and if the GPPGA total score of of the patient persists after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

17.A method of treating a GPP patient with a GPP Physician Global Assessment (GPPGA) total score of and a GPPGA pustulation subscore of 2, said method comprising the steps of: (a) administering to the patient a first 900 mg intravenous (I.V.) dose of an anti-IL-36R antibody; (b) assessing the GPPGA scores of the patient and if the GPPGA total score of 2 and the GPPGA pustulation subscore of 2 of the patient persist after 1 week from the first dose, then administering to the patient a second 900 mg (i.v.) dose of spesolimab less than 2 weeks after the first dose.

18.The method according to any of the preceding claims, wherein an optional third 900 mg i.v. dose of the anti-IL-36R antibody is administered at 2 to 12 weeks after the second i.v. dose.

19.The method according to any of the preceding claims, wherein the two-dose administration achieves one or more of the following results in:
(a) a Generalized Pustular Psoriasis Global Assessment (GPPGA) pustulation subscore of 0 indicating in one week after administering the second i.v. dose;
(b) a GPPGA total score of 0 or 1 in one week after administering the second i.v.
dose; and/or (c) a lower maximum ADA titer at 2, 4, 6, 8, 10 or 12 weeks after administering the second i.v. dose.

20.The method of claim 19, wherein the results are maintained for up to and at least 12 weeks following the administration of the second i.v. dose.

21.The method according to any of the preceding claims, wherein the method further comprises administering to the patient a prophylactically effective amount of the anti-IL-36R antibody in one or more subcutaneous doses after the last i.v. dose administered.

22.The method according claim 21, wherein each of the one or more subcutaneous doses comprises 150 mg, 225 mg, 300 mg, 450 mg, or 600 mg of said anti-IL-36R
antibody.

23.The method according to claim 22, wherein 1, 2, 3 or more subcutaneous doses are administered to the patient and wherein a first subcutaneous dose is administered after the last intravenous dose.

24.The method according to claim 23, wherein a first subcutaneous dose is administered 2 to 8 weeks, 4 to 6 weeks, 2 weeks, 4 weeks, 6 weeks or 8 weeks, weeks, 16 weeks, 20 weeks after the last intravenous dose, and subsequent subcutaneous doses are administered at 2, 4, 6, 8, 10 or 12 weeks intervals after the first subcutaneous dose.

25. The method according to any of the preceding claims, wherein the patient remains in clinical remission as measured by a GPPGA total score of 0 or 1 for at least 12, 24, 36, 48, 60 or 72 weeks following the last i.v. or subcutaneous dose.

26.The method according to any of the preceding claims, wherein the anti-IL-antibody comprises: a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID NO: 35, 102, 103, 104, 105 106 or 140 (L-CDR2); the amino acid sequence of SEQ ID NO:
44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).

27.The method according to any of the preceding claims, wherein the anti-IL-antibody comprises:
I. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 102 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
II. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 103 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
III. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 104 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
IV. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 105 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
V. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 106 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).
VI. a) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 26 (L-CDR1); the amino acid sequence of SEQ ID
NO: 140 (L-CDR2); the amino acid sequence of SEQ ID NO: 44 (L-CDR3); and b) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 53 (H-CDR1); the amino acid sequence of SEQ ID NO: 62, 108, 109, 110 or 111 (H-CDR2); the amino acid sequence of SEQ ID NO: 72 (H-CDR3).

28.The method according to any of the preceding claims, wherein the anti-IL-antibody comprises:
(i) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 87;or (ii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 88; or (iii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 77; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 89; or (iv) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 87;or (v) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 88; or (vi) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 80; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 89; or (vii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 85; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 100; or (viii) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 85; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:101; or (ix) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 86; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 100; or (x) a light chain variable region comprising the amino acid sequence of SEQ ID NO: 86; and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:101.

29.The method according to any of the preceding claims, wherein the anti-IL-antibody comprises:
i. a light chain comprising the amino acid sequence of SEQ ID NO:
115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 125; or ii. a light chain comprising the amino acid sequence of SEQ ID NO:
115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 126; or iii. a light chain comprising the amino acid sequence of SEQ ID NO:
115; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 127; or iv. a light chain comprising the amino acid sequence of SEQ ID NO:
118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 125; or v. a light chain comprising the amino acid sequence of SEQ ID NO:
118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 126; or vi. a light chain comprising the amino acid sequence of SEQ ID NO:
118; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 127; or vii. a light chain comprising the amino acid sequence of SEQ ID NO:
123; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 138; or viii. a light chain comprising the amino acid sequence of SEQ ID NO:
123; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 139; or ix. a light chain comprising the amino acid sequence of SEQ ID NO:
124; and a heavy chain comprising the amino acid sequence of SEQ ID NO: 138.

30. The method according to any of the preceding claims, wherein the anti-IL-antibody is spesolimab.