CA3204355A1 - Biocapteur electrochimique jetable base sur la deshydrogenase et la diaphorase dependantes de nad(p) - Google Patents

Biocapteur electrochimique jetable base sur la deshydrogenase et la diaphorase dependantes de nad(p)

Info

Publication number
CA3204355A1
CA3204355A1 CA3204355A CA3204355A CA3204355A1 CA 3204355 A1 CA3204355 A1 CA 3204355A1 CA 3204355 A CA3204355 A CA 3204355A CA 3204355 A CA3204355 A CA 3204355A CA 3204355 A1 CA3204355 A1 CA 3204355A1
Authority
CA
Canada
Prior art keywords
reagent
nad
dehydrogenase
base layer
electrode
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3204355A
Other languages
English (en)
Inventor
Jianhong Pei
Joseph Bedard
Aaron Tram
Samantha Mosley
Chung Chang Young
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nova Biomedical Corp
Original Assignee
Nova Biomedical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nova Biomedical Corp filed Critical Nova Biomedical Corp
Publication of CA3204355A1 publication Critical patent/CA3204355A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90209—Oxidoreductases (1.) acting on NADH or NADPH (1.6), e.g. those with a heme protein as acceptor (1.6.2) (general), Cytochrome-b5 reductase (1.6.2.2) or NADPH-cytochrome P450 reductase (1.6.2.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90212—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Biocapteur jetable comprenant une surface de détection ayant au moins une partie d'électrode de travail et une partie d'électrode de référence, un premier réactif disposé sur la partie d'électrode de travail contenant une déshydrogénase dépendante de NAD(P), NAD(P)+, une diaphorase, et une forme oxydée d'un médiateur redox formant une électrode de travail, et un matériau d'électrode de référence disposé sur la partie d'électrode de référence formant une électrode de référence.
CA3204355A 2021-02-25 2021-02-25 Biocapteur electrochimique jetable base sur la deshydrogenase et la diaphorase dependantes de nad(p) Pending CA3204355A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2021/019515 WO2022182344A1 (fr) 2021-02-25 2021-02-25 Biocapteur électrochimique jetable basé sur la déshydrogénase et la diaphorase dépendantes de nad(p)

Publications (1)

Publication Number Publication Date
CA3204355A1 true CA3204355A1 (fr) 2022-09-01

Family

ID=83049562

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3204355A Pending CA3204355A1 (fr) 2021-02-25 2021-02-25 Biocapteur electrochimique jetable base sur la deshydrogenase et la diaphorase dependantes de nad(p)

Country Status (7)

Country Link
EP (1) EP4298234A1 (fr)
JP (1) JP2024508623A (fr)
KR (1) KR20230150262A (fr)
CN (1) CN117616132A (fr)
CA (1) CA3204355A1 (fr)
MX (1) MX2023008281A (fr)
WO (1) WO2022182344A1 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5508171A (en) * 1989-12-15 1996-04-16 Boehringer Mannheim Corporation Assay method with enzyme electrode system
US8617366B2 (en) * 2005-12-12 2013-12-31 Nova Biomedical Corporation Disposable urea sensor and system for determining creatinine and urea nitrogen-to-creatinine ratio in a single device
US20170121754A1 (en) * 2014-06-20 2017-05-04 Abbott Diabetes Care Inc. Test Strip, Meter, and Method for Assaying Enzyme Activity
SI3423591T1 (sl) * 2016-03-04 2024-03-29 Abbott Diabetes Care Inc., Od NAD(P) odvisni odzivni encimi, elektrode in senzorji ter postopki za izdelavo in uporabo le-teh
US20210219876A1 (en) * 2020-01-21 2021-07-22 Nova Biomedical Corporation Disposable electrochemical biosensor based on NAD(P)-dependent dehydrogenase and diaphorase

Also Published As

Publication number Publication date
MX2023008281A (es) 2023-10-09
EP4298234A1 (fr) 2024-01-03
WO2022182344A1 (fr) 2022-09-01
KR20230150262A (ko) 2023-10-30
CN117616132A (zh) 2024-02-27
JP2024508623A (ja) 2024-02-28

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