CA3196827A1 - Compositions and methods for inhibiting gene expression - Google Patents
Compositions and methods for inhibiting gene expressionInfo
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Abstract
The present disclosure relates to expression repressors and expression repression systems for modulating expression of a target gene in a cell.
Description
COMPOSITIONS AND METHODS FOR INHIBITING GENE EXPRESSION
CROSS-REFERENCE TO RELATED APPLICATIONS
The present application claims the benefit of U.S. provisional applications 63/082,555 filed September 24, 2020 and 63/242,957 filed September 10, 2021. The contents of the aforementioned applications are hereby incorporated by reference in their entirety.
SEQUENCE LISTING
The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on September 17, 2021, is named 02057-7017W0_SL.txt and is 694,831 bytes in size.
BACKGROUND
Mis regulation of gene expression is the underlying cause of many diseases (e.g., in mammals, e.g., humans). While techniques exist that modulate gene expression for short periods of time, the treatment of many diseases calls for stable, long-term modulation of gene expression. There is a need for novel tools, systems, and methods to stably alter, e.g., decrease, expression of disease associated genes.
SUMMARY
The disclosure provides, among other things, expression repressors and expression repression systems that may be used to modulate, e.g., decrease, expression of a target gene.
In some aspects, an expression repressor comprises a DNA targeting moiety and a repressor domain capable of modulating (e.g., decreasing) the expression of a target gene. In some embodiments, an expression repressor comprises a DNA targeting moiety, a first repressor domain, and a second repressor domain. In some embodiments, the first repressor domain is different from the second repressor domain.
In some embodiments, the first repressor domain is identical to the second repressor domain.
In some aspects, the disclosure features an expression repression system comprising a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain. In some embodiments, the first DNA-targeting moiety specifically binds a first DNA
sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence.
In some embodiments, the first repressor domain is different from the second repressor domain. In some embodiments, an expression repression system comprises: (i) a first expression repressor comprising a first DNA-targeting moiety, a first repressor domain, and a second repressor domain, and (ii) a second
CROSS-REFERENCE TO RELATED APPLICATIONS
The present application claims the benefit of U.S. provisional applications 63/082,555 filed September 24, 2020 and 63/242,957 filed September 10, 2021. The contents of the aforementioned applications are hereby incorporated by reference in their entirety.
SEQUENCE LISTING
The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on September 17, 2021, is named 02057-7017W0_SL.txt and is 694,831 bytes in size.
BACKGROUND
Mis regulation of gene expression is the underlying cause of many diseases (e.g., in mammals, e.g., humans). While techniques exist that modulate gene expression for short periods of time, the treatment of many diseases calls for stable, long-term modulation of gene expression. There is a need for novel tools, systems, and methods to stably alter, e.g., decrease, expression of disease associated genes.
SUMMARY
The disclosure provides, among other things, expression repressors and expression repression systems that may be used to modulate, e.g., decrease, expression of a target gene.
In some aspects, an expression repressor comprises a DNA targeting moiety and a repressor domain capable of modulating (e.g., decreasing) the expression of a target gene. In some embodiments, an expression repressor comprises a DNA targeting moiety, a first repressor domain, and a second repressor domain. In some embodiments, the first repressor domain is different from the second repressor domain.
In some embodiments, the first repressor domain is identical to the second repressor domain.
In some aspects, the disclosure features an expression repression system comprising a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain. In some embodiments, the first DNA-targeting moiety specifically binds a first DNA
sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence.
In some embodiments, the first repressor domain is different from the second repressor domain. In some embodiments, an expression repression system comprises: (i) a first expression repressor comprising a first DNA-targeting moiety, a first repressor domain, and a second repressor domain, and (ii) a second
2 expression repressor comprising a second DNA-targeting moiety and a first repressor domain. In some embodiments, all three of the repressor domains are different. In some embodiments, at least two repressor domains are identical. In some embodiments, an expression repression system comprises: (i) a first expression repressor comprising a first DNA-targeting moiety, a first repressor domain, and a second repressor domain, and (ii) a second expression repressor comprising a second DNA-targeting moiety, a first repressor domain and a second repressor domain. In some embodiments, all four of the repressor domains are different. In some embodiments, at least two repressor domains are identical.
Generally, modulation of expression of a target gene by an expression repression system involves the binding of the first expression repressor and second expression repressor to the first and second DNA
sequences, respectively. Binding of the first and second DNA sequences localizes the functionalities of the first and second repressor domains to those sites. Without wishing to be bound by theory, in some embodiments it is thought that employing the functionalities of both the first and second repressor domains stably represses expression of a target gene associated with or comprising the first and/or second DNA sequences, e.g., wherein the first and/or second DNA sequences are or comprise sequences of the target gene or one or more operably linked transcription control elements.
In some aspects, the disclosure provides an expression repressor or an expression repression system comprising: a targeting moiety that binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of SEQ ID NO: 1-21, wherein the expression repressor or the expression repression system is capable of decreasing the expression of a target gene.
In some aspects, the disclosure provides an expression repressor comprising: a DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds to a transcription regulatory element (e.g., a promoter or transcription start site (TSS)) operably linked to a target gene, or a sequence proximal to said transcription regulatory element; and a repressor domain. In some embodiments, an expression repressor comprises a DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds to a transcription regulatory element (e.g., a promoter or transcription start site (TSS)) operably linked to a target gene, or a sequence proximal to said transcription regulatory element, a first repressor domain, and a second repressor domain. In some embodiments, the first repressor domain is identical to the second repressor domain. In some embodiments, the first repressor domain is not identical to the second repressor domain.
In some aspects, the disclosure provides an expression repression system comprising: (i) a first expression repressor comprising a first DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds
Generally, modulation of expression of a target gene by an expression repression system involves the binding of the first expression repressor and second expression repressor to the first and second DNA
sequences, respectively. Binding of the first and second DNA sequences localizes the functionalities of the first and second repressor domains to those sites. Without wishing to be bound by theory, in some embodiments it is thought that employing the functionalities of both the first and second repressor domains stably represses expression of a target gene associated with or comprising the first and/or second DNA sequences, e.g., wherein the first and/or second DNA sequences are or comprise sequences of the target gene or one or more operably linked transcription control elements.
In some aspects, the disclosure provides an expression repressor or an expression repression system comprising: a targeting moiety that binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of SEQ ID NO: 1-21, wherein the expression repressor or the expression repression system is capable of decreasing the expression of a target gene.
In some aspects, the disclosure provides an expression repressor comprising: a DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds to a transcription regulatory element (e.g., a promoter or transcription start site (TSS)) operably linked to a target gene, or a sequence proximal to said transcription regulatory element; and a repressor domain. In some embodiments, an expression repressor comprises a DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds to a transcription regulatory element (e.g., a promoter or transcription start site (TSS)) operably linked to a target gene, or a sequence proximal to said transcription regulatory element, a first repressor domain, and a second repressor domain. In some embodiments, the first repressor domain is identical to the second repressor domain. In some embodiments, the first repressor domain is not identical to the second repressor domain.
In some aspects, the disclosure provides an expression repression system comprising: (i) a first expression repressor comprising a first DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds
3 PCT/US2021/051945 to a transcription regulatory element (e.g., a promoter or transcription start site (TSS)) operably linked to a target gene, or a sequence proximal to said transcription regulatory element; and a first repressor domain and a second expression repressor comprising a second DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds to a transcription regulatory element (e.g., a promoter or transcription start site (TSS)) operably linked to a target gene, or a sequence proximal to said transcription regulatory element; and a first repressor domain. In some embodiments, the first repressor domains of the first expression repressor and the second expression repressors are identical. In some embodiments, the first repression domains of the first and the second expression pressors are different. In some embodiments, .. the first DNA-targeting moiety and the second DNA-targeting moiety are identical. In some embodiments, the first DNA-targeting moiety and the second DNA-targeting moiety are different. In some embodiments, an expression repression system comprises a first expression repressor comprising a first DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds to a transcription regulatory element (e.g., a promoter or transcription start site (TSS)) operably linked to a target gene, or a sequence proximal to said transcription regulatory element, a first repressor domain, and a second repressor domain and a second expression repressor comprising a second DNA-targeting moiety (wherein optionally the DNA-targeting moiety comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/Cas protein), that binds to a transcription regulatory element (e.g., a promoter or transcription start site (TSS)) operably linked to a target gene, or a sequence proximal to said transcription regulatory element; a first repressor domain and a second repressor domain. In some embodiments, the first repressor domains of the first expression repressor and the second expression repressors are identical.
In some embodiments, the first repression domains of the first and the second expression pressors are different. In some embodiments, the second repressor domains of the first expression repressor and the second expression repressors are identical. In some embodiments, the second repression domains of the first and the second expression pressors are different.
In some aspects, the disclosure features a nucleic acid encoding an expression repressor or a component thereof (e.g., a gRNA). In some aspects, the disclosure is directed to a nucleic acid encoding the first expression repressor, second expression repressor, both, or a component thereof (e.g., a gRNA).
In some aspects, the disclosure is directed to a vector comprising a nucleic acid described herein. In some aspects, the disclosure is directed to a cell comprising an expression repressor, an expression repression system, nucleic acid, or vector described herein. In some aspects, the disclosure is directed to a lipid nanoparticle comprising a vector, a nucleic acid, an expression repression system, or an expression repressor described herein. In some aspects, the disclosure is directed to a reaction mixture comprising an
In some embodiments, the first repression domains of the first and the second expression pressors are different. In some embodiments, the second repressor domains of the first expression repressor and the second expression repressors are identical. In some embodiments, the second repression domains of the first and the second expression pressors are different.
In some aspects, the disclosure features a nucleic acid encoding an expression repressor or a component thereof (e.g., a gRNA). In some aspects, the disclosure is directed to a nucleic acid encoding the first expression repressor, second expression repressor, both, or a component thereof (e.g., a gRNA).
In some aspects, the disclosure is directed to a vector comprising a nucleic acid described herein. In some aspects, the disclosure is directed to a cell comprising an expression repressor, an expression repression system, nucleic acid, or vector described herein. In some aspects, the disclosure is directed to a lipid nanoparticle comprising a vector, a nucleic acid, an expression repression system, or an expression repressor described herein. In some aspects, the disclosure is directed to a reaction mixture comprising an
4 expression repressor, an expression repression system, a nucleic acid, a vector, or a lipid nanoparticle described herein. In some aspects, the disclosure is directed to a pharmaceutical composition comprising an expression repression system, nucleic acid, or vector described herein.
In some aspects, the disclosure is directed to a method of decreasing expression of a target gene comprising providing an expression repressor or an expression repression system described herein and contacting the target gene and/or one or more operably linked transcription control elements with the expression repressor or the expression repression system, thereby decreasing expression of the target gene.
In some aspects, the disclosure is directed to a method of treating a condition associated with over-expression of a target gene in a subject, comprising administering an expression repressor or an expression repression system, nucleic acid, or vector described herein to the subject, thereby treating the condition.
In some aspects, the disclosure is directed to a method of treating a condition associated with mis-regulation of a target gene in a subject, comprising administering an expression repressor or an expression repression system, nucleic acid, or vector described herein to the subject, thereby treating the condition.
Additional features of any of the aforesaid methods or compositions include one or more of the following enumerated embodiments.
Those skilled in the art will recognize or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following enumerated embodiments.
All publications, patent applications, patents, and other references (e.g., sequence database reference numbers) mentioned herein are incorporated by reference in their entirety. For example, all GenBank, Unigene, and Entrez sequences referred to herein, e.g., in any Table herein, are incorporated by reference. Unless otherwise specified, the sequence accession numbers specified herein, including in any Table herein, refer to the database entries current as of September 23, 2019.
When one gene or protein references a plurality of sequence accession numbers, all of the sequence variants are encompassed.
ENUMERATED EMBODIMENTS
1. An expression repressor comprising:
a DNA-targeting moiety and a repressor domain, wherein the expression repressor is capable of decreasing expression of a target gene.
2. The expression repressor of embodiment 1, wherein the DNA-targeting moiety binds to a transcription regulatory element (e.g., a promoter, an enhancer, a super enhancer, or transcription start site (TSS)) operably linked to the target gene or binds to a sequence proximal to said transcription regulatory element.
In some aspects, the disclosure is directed to a method of decreasing expression of a target gene comprising providing an expression repressor or an expression repression system described herein and contacting the target gene and/or one or more operably linked transcription control elements with the expression repressor or the expression repression system, thereby decreasing expression of the target gene.
In some aspects, the disclosure is directed to a method of treating a condition associated with over-expression of a target gene in a subject, comprising administering an expression repressor or an expression repression system, nucleic acid, or vector described herein to the subject, thereby treating the condition.
In some aspects, the disclosure is directed to a method of treating a condition associated with mis-regulation of a target gene in a subject, comprising administering an expression repressor or an expression repression system, nucleic acid, or vector described herein to the subject, thereby treating the condition.
Additional features of any of the aforesaid methods or compositions include one or more of the following enumerated embodiments.
Those skilled in the art will recognize or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following enumerated embodiments.
All publications, patent applications, patents, and other references (e.g., sequence database reference numbers) mentioned herein are incorporated by reference in their entirety. For example, all GenBank, Unigene, and Entrez sequences referred to herein, e.g., in any Table herein, are incorporated by reference. Unless otherwise specified, the sequence accession numbers specified herein, including in any Table herein, refer to the database entries current as of September 23, 2019.
When one gene or protein references a plurality of sequence accession numbers, all of the sequence variants are encompassed.
ENUMERATED EMBODIMENTS
1. An expression repressor comprising:
a DNA-targeting moiety and a repressor domain, wherein the expression repressor is capable of decreasing expression of a target gene.
2. The expression repressor of embodiment 1, wherein the DNA-targeting moiety binds to a transcription regulatory element (e.g., a promoter, an enhancer, a super enhancer, or transcription start site (TSS)) operably linked to the target gene or binds to a sequence proximal to said transcription regulatory element.
5 3. The expression repressor of embodiment 1 or 2, wherein the repressor domain is covalently linked to the DNA-targeting moiety.
4. The expression repressor of embodiments 1 or 2, wherein the repressor domain is linked to the DNA-targeting moiety via a linker.
5. The expression repressor of any of embodiment1-4, wherein the repressor domain is C-terminal of the DNA-targeting moiety.
4. The expression repressor of embodiments 1 or 2, wherein the repressor domain is linked to the DNA-targeting moiety via a linker.
5. The expression repressor of any of embodiment1-4, wherein the repressor domain is C-terminal of the DNA-targeting moiety.
6. The expression repressor of any of embodiments 1-4, wherein the repressor domain is N-terminal of the DNA-targeting moiety.
7. The expression repressor of any of embodiments1-6, wherein the repressor domain is encoded by a nucleotide sequence chosen from any of SEQ ID NOs: 47-56, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
8. The expression repressor of any of embodiments1-7, wherein the repressor domain comprises an amino acid sequence according to any of SEQ ID NOs: 57-66, 90 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
9. The expression repressor of any of the preceding embodiments, wherein the repressor domain is MQ1 or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 57 or 90 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
10. The expression repressor of any of the preceding embodiments, wherein the repressor domain is DNMT1 or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 58 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
11. The expression repressor of any of the preceding embodiments, wherein the repressor domain is DNMT3a/3L, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 59 or 60 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
.. 12. The expression repressor of any of the preceding embodiments, wherein the repressor domain is KRAB, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 61 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
13. The expression repressor of any of the preceding embodiments, wherein the repressor domain is G9A, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 62 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or .. having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is N-terminal of the DNA- targeting moiety.
14. The expression repressor of any of the preceding embodiments, wherein the repressor domain is HDAC8, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 63 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
15. The expression repressor of any of the preceding embodiments, wherein the repressor domain is LSD1, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 64 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
16. The expression repressor of any of the preceding embodiments, wherein the repressor domain is EZH2, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 64 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is N-terminal of the DNA-targeting moiety.
17. The expression repressor of any of the preceding embodiments, wherein the repressor domain is FOG1, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 66 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
18. The expression repressor of any of the preceding embodiments, which further comprises a second repressor domain.
19. The expression repressor of embodiment 18, wherein the DNA-targeting moiety is situated between the repressor domain and the second repressor domain.
20. An expression repressor comprising:
a DNA-targeting moiety, a first repressor domain and a second repressor domain, wherein the expression repressor is capable of decreasing expression of a target gene.
21. The expression repressor of any preceding embodiment, wherein the DNA-targeting moiety binds to a transcription regulatory element (e.g., a promoter, an enhancer, a super enhancer, or transcription start site (TSS)) operably linked to the target gene or binds to a sequence proximal to said transcription regulatory element.
22. The expression repressor of any of embodiments 18-21, wherein the first repressor domain is identical to the second repressor domain.
23. The expression repressor of any of embodiments 18-21, wherein the first repressor domain is not identical to the second repressor domain.
24. The expression repressor of any of embodiments 18-23, wherein the first repressor domain and the second repressor domain are covalently linked to the DNA-targeting moiety.
25. The expression repressor of any of embodiments18-24, wherein the first repressor domain is linked to the DNA-targeting moiety via a first linker and the second repressor domain is linked to the DNA-targeting moiety via a second linker.
26. The expression repressor of embodiment 25, wherein the first linker is identical to the second linker.
27. The expression repressor of embodiment 25, wherein the first linker is not identical to the second linker.
28. The expression repressor of any of embodiments 18-27, wherein the first repressor domain is N-terminal of the DNA-targeting moiety.
29. The expression repressor of any of embodiments 18-28, wherein the second repressor domain is C-terminal of the DNA-targeting moiety.
30. The expression repressor of any of embodiments 25-29, wherein the C-terminal end of the first repressor domain is linked to the N-terminal end of the DNA-targeting moiety via the first linker and the N-terminal end of the second repressor domain is linked to the C-terminal end of the DNA-targeting moiety via the second linker.
31. The expression repressor of any of embodiments 18-30, wherein:
the first repressor domain is EZH2, or a functional variant or fragment thereof, e.g., wherein the first repressor domain comprises an amino acid sequence of SEQ ID NO: 64 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the first repressor domain is N-terminal of the DNA-targeting moiety; and the second repressor domain is KRAB, or a functional variant or fragment thereof, e.g., wherein the second repressor domain comprises an amino acid sequence of SEQ ID NO: 61 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13,
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
.. 12. The expression repressor of any of the preceding embodiments, wherein the repressor domain is KRAB, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 61 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
13. The expression repressor of any of the preceding embodiments, wherein the repressor domain is G9A, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 62 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or .. having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is N-terminal of the DNA- targeting moiety.
14. The expression repressor of any of the preceding embodiments, wherein the repressor domain is HDAC8, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 63 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
15. The expression repressor of any of the preceding embodiments, wherein the repressor domain is LSD1, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 64 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is C-terminal of the DNA-targeting moiety.
16. The expression repressor of any of the preceding embodiments, wherein the repressor domain is EZH2, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 64 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, wherein optionally the repressor domain is N-terminal of the DNA-targeting moiety.
17. The expression repressor of any of the preceding embodiments, wherein the repressor domain is FOG1, or a functional variant or fragment thereof, e.g., wherein the repressor domain comprises an amino acid sequence of SEQ ID NO: 66 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
18. The expression repressor of any of the preceding embodiments, which further comprises a second repressor domain.
19. The expression repressor of embodiment 18, wherein the DNA-targeting moiety is situated between the repressor domain and the second repressor domain.
20. An expression repressor comprising:
a DNA-targeting moiety, a first repressor domain and a second repressor domain, wherein the expression repressor is capable of decreasing expression of a target gene.
21. The expression repressor of any preceding embodiment, wherein the DNA-targeting moiety binds to a transcription regulatory element (e.g., a promoter, an enhancer, a super enhancer, or transcription start site (TSS)) operably linked to the target gene or binds to a sequence proximal to said transcription regulatory element.
22. The expression repressor of any of embodiments 18-21, wherein the first repressor domain is identical to the second repressor domain.
23. The expression repressor of any of embodiments 18-21, wherein the first repressor domain is not identical to the second repressor domain.
24. The expression repressor of any of embodiments 18-23, wherein the first repressor domain and the second repressor domain are covalently linked to the DNA-targeting moiety.
25. The expression repressor of any of embodiments18-24, wherein the first repressor domain is linked to the DNA-targeting moiety via a first linker and the second repressor domain is linked to the DNA-targeting moiety via a second linker.
26. The expression repressor of embodiment 25, wherein the first linker is identical to the second linker.
27. The expression repressor of embodiment 25, wherein the first linker is not identical to the second linker.
28. The expression repressor of any of embodiments 18-27, wherein the first repressor domain is N-terminal of the DNA-targeting moiety.
29. The expression repressor of any of embodiments 18-28, wherein the second repressor domain is C-terminal of the DNA-targeting moiety.
30. The expression repressor of any of embodiments 25-29, wherein the C-terminal end of the first repressor domain is linked to the N-terminal end of the DNA-targeting moiety via the first linker and the N-terminal end of the second repressor domain is linked to the C-terminal end of the DNA-targeting moiety via the second linker.
31. The expression repressor of any of embodiments 18-30, wherein:
the first repressor domain is EZH2, or a functional variant or fragment thereof, e.g., wherein the first repressor domain comprises an amino acid sequence of SEQ ID NO: 64 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the first repressor domain is N-terminal of the DNA-targeting moiety; and the second repressor domain is KRAB, or a functional variant or fragment thereof, e.g., wherein the second repressor domain comprises an amino acid sequence of SEQ ID NO: 61 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13,
12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the second repressor domain is C-terminal of the DNA-targeting moiety.
32. The expression repressor of any of embodiments 18-30, wherein:
the first repressor domain is G9A, or a functional variant or fragment thereof, e.g., wherein the first repressor domain comprises an amino acid sequence of SEQ ID NO: 62 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the first repressor domain is N-terminal of the DNA-targeting moiety; and the second repressor domain is KRAB, or a functional variant or fragment thereof, e.g., wherein the second repressor domain comprises an amino acid sequence of SEQ ID NO: 61 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the second repressor domain is C-terminal of the DNA-targeting moiety.
33. The expression repressor of any of embodiments 18-30, wherein:
the first repressor domain is FOG1, or a functional variant or fragment thereof, e.g., wherein the first repressor domain comprises an amino acid sequence of SEQ ID NO: 66 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the first repressor domain is N-terminal of the DNA-targeting moiety; and the second repressor domain is FOG1, or a functional variant or fragment thereof, e.g., wherein the second repressor domain comprises an amino acid sequence of SEQ ID NO: 66 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the second repressor domain is C-terminal of the DNA-targeting moiety.
34. The expression repressor of any of embodiments 1-33, wherein the DNA-targeting moiety binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ
ID NO: 1-21.
5 35. The expression repressor of any of embodiments 1-34, wherein the DNA-targeting moiety comprises a CRISPR/Cas molecule (e.g., a catalytically inactive CRISPR/Cas protein), a zinc finger domain, or a TAL effector molecule.
36. The expression repressor of embodiment 35, wherein the CRISPR/Cas molecule comprises one or 10 more mutations selected from DlOA, D839A, H840A, and N863A.
37. The expression repressor of any of embodiments 1-36, wherein the DNA-targeting moiety binds to a promoter region of a target gene, an enhancer region (e.g., a super enhancer region), or an anchor sequence.
38. The expression repressor of any of embodiments 1-37, wherein the repressor domain comprises DNMT1, DNMT3a/3L, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1, 39. The expression repressor of any of embodiments 18-38, wherein the first repressor domain comprising, DNMT1, DNMT3a/31, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1, and the second repressor domain comprises DNMT1, DNMT3a/31, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1, 40. The expression repressor of any of embodiments 1-39, wherein the expression repressor is encoded by a nucleotide sequence chosen from any of SEQ ID NOs: 22-32, 92, 95 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
41. The expression repressor of any of embodiments 1-40, wherein the expression repressor comprises an amino acid sequence chosen from any of SEQ ID Nos: 33-36, 38-44, 67-69, 93, 96, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
42. The expression repressor of any of embodiments1-41, wherein the DNA-targeting moiety is encoded by a nucleotide sequence chosen from any of SEQ ID NOs: 45 or 89 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
43. The expression repressor of any of embodiments 1-42, wherein the DNA-targeting moiety comprises an amino acid sequence according to any of SEQ ID NOs: 46 or 88, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
44. The expression repressor of any of embodiments 18-43, wherein, (i) the first repressor domain is encoded by a nucleotide sequence chosen from any of SEQ ID
NOs: 47-56, 90 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and (ii) the second repressor domain is encoded by a nucleotide sequence chosen from any of SEQ ID
NOs: 47-56, 90 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
45. The expression repressor of any of embodiments 18-43, wherein, (i) the first repressor domain comprises an amino acid sequence according to any of SEQ ID
NOs: 57-66, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and (ii) the second repressor domain comprises an amino acid sequence according to any of SEQ ID
NOs: 57-66, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
46. The expression repressor of any preceding embodiments, wherein the DNA-targeting moiety comprises a TAL effector molecule, a CRISPR/Cas molecule, a zinc finger domain, a tetR domain, a meganuclease domain, or an oligonucleotide.
47. The expression repressor of any preceding embodiments, wherein the DNA-targeting moiety further comprises a gRNA, e.g., a gRNA that binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ ID NOs: 1-21, e.g., wherein the gRNA comprises a sequence that comprises at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ
ID NOs: 1-21.
48. The expression repressor of any of embodiments 35-47, wherein the DNA-targeting domain comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/ Cas protein, and a gRNA, e.g., a gRNA that binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ ID NOs: 1-21, e.g., wherein the gRNA comprises a sequence that comprises at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ
ID NOs: 1-21 and the repressor domain comprises a moiety chosen from DNMT1, DNMt3a/31, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1.
49. The expression repressor of embodiment 35-47, wherein the DNA-targeting domain comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/ Cas protein, and a gRNA, e.g., a gRNA that binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ ID NOs: 1-21, e.g., wherein the gRNA comprises a sequence that comprises at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ ID NOs: 1-21, the first repressor domain comprises a moiety chosen from DNMT1, DNM T3a/3L, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1 and the second domain comprises a moiety chosen from DNMT1, DNMt3a/3L, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1.
50. The expression repressor of any of embodiments 35-49, wherein the CRISPR/Cas molecule comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof.
51. The expression repressor of any of embodiments 35-50, wherein the CRISPR/Cas molecule comprises a catalytically inactive CRISPR/Cas protein, e.g., dCas9.
52. The expression repressor of any of the preceding embodiments, which: (i) comprises one or more nuclear localization signal sequences (NLS), or (ii) does not comprise an NLS.
53. The expression repressor of any of the preceding embodiments, comprising a first NLS at the N
terminus, e.g., wherein the first NLS has a sequence of SEQ ID NO: 86.
54. The expression repressor of any of the preceding embodiments, comprising an NLS, e.g., a second NLS, at the C terminus, e.g., having a sequence of SEQ ID NO: 87.
32. The expression repressor of any of embodiments 18-30, wherein:
the first repressor domain is G9A, or a functional variant or fragment thereof, e.g., wherein the first repressor domain comprises an amino acid sequence of SEQ ID NO: 62 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the first repressor domain is N-terminal of the DNA-targeting moiety; and the second repressor domain is KRAB, or a functional variant or fragment thereof, e.g., wherein the second repressor domain comprises an amino acid sequence of SEQ ID NO: 61 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the second repressor domain is C-terminal of the DNA-targeting moiety.
33. The expression repressor of any of embodiments 18-30, wherein:
the first repressor domain is FOG1, or a functional variant or fragment thereof, e.g., wherein the first repressor domain comprises an amino acid sequence of SEQ ID NO: 66 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the first repressor domain is N-terminal of the DNA-targeting moiety; and the second repressor domain is FOG1, or a functional variant or fragment thereof, e.g., wherein the second repressor domain comprises an amino acid sequence of SEQ ID NO: 66 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and the second repressor domain is C-terminal of the DNA-targeting moiety.
34. The expression repressor of any of embodiments 1-33, wherein the DNA-targeting moiety binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ
ID NO: 1-21.
5 35. The expression repressor of any of embodiments 1-34, wherein the DNA-targeting moiety comprises a CRISPR/Cas molecule (e.g., a catalytically inactive CRISPR/Cas protein), a zinc finger domain, or a TAL effector molecule.
36. The expression repressor of embodiment 35, wherein the CRISPR/Cas molecule comprises one or 10 more mutations selected from DlOA, D839A, H840A, and N863A.
37. The expression repressor of any of embodiments 1-36, wherein the DNA-targeting moiety binds to a promoter region of a target gene, an enhancer region (e.g., a super enhancer region), or an anchor sequence.
38. The expression repressor of any of embodiments 1-37, wherein the repressor domain comprises DNMT1, DNMT3a/3L, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1, 39. The expression repressor of any of embodiments 18-38, wherein the first repressor domain comprising, DNMT1, DNMT3a/31, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1, and the second repressor domain comprises DNMT1, DNMT3a/31, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1, 40. The expression repressor of any of embodiments 1-39, wherein the expression repressor is encoded by a nucleotide sequence chosen from any of SEQ ID NOs: 22-32, 92, 95 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
41. The expression repressor of any of embodiments 1-40, wherein the expression repressor comprises an amino acid sequence chosen from any of SEQ ID Nos: 33-36, 38-44, 67-69, 93, 96, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
42. The expression repressor of any of embodiments1-41, wherein the DNA-targeting moiety is encoded by a nucleotide sequence chosen from any of SEQ ID NOs: 45 or 89 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
43. The expression repressor of any of embodiments 1-42, wherein the DNA-targeting moiety comprises an amino acid sequence according to any of SEQ ID NOs: 46 or 88, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
44. The expression repressor of any of embodiments 18-43, wherein, (i) the first repressor domain is encoded by a nucleotide sequence chosen from any of SEQ ID
NOs: 47-56, 90 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and (ii) the second repressor domain is encoded by a nucleotide sequence chosen from any of SEQ ID
NOs: 47-56, 90 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
45. The expression repressor of any of embodiments 18-43, wherein, (i) the first repressor domain comprises an amino acid sequence according to any of SEQ ID
NOs: 57-66, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto, and (ii) the second repressor domain comprises an amino acid sequence according to any of SEQ ID
NOs: 57-66, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
46. The expression repressor of any preceding embodiments, wherein the DNA-targeting moiety comprises a TAL effector molecule, a CRISPR/Cas molecule, a zinc finger domain, a tetR domain, a meganuclease domain, or an oligonucleotide.
47. The expression repressor of any preceding embodiments, wherein the DNA-targeting moiety further comprises a gRNA, e.g., a gRNA that binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ ID NOs: 1-21, e.g., wherein the gRNA comprises a sequence that comprises at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ
ID NOs: 1-21.
48. The expression repressor of any of embodiments 35-47, wherein the DNA-targeting domain comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/ Cas protein, and a gRNA, e.g., a gRNA that binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ ID NOs: 1-21, e.g., wherein the gRNA comprises a sequence that comprises at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ
ID NOs: 1-21 and the repressor domain comprises a moiety chosen from DNMT1, DNMt3a/31, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1.
49. The expression repressor of embodiment 35-47, wherein the DNA-targeting domain comprises a CRISPR/Cas molecule, e.g., a catalytically inactive CRISPR/ Cas protein, and a gRNA, e.g., a gRNA that binds a genomic locus comprising at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ ID NOs: 1-21, e.g., wherein the gRNA comprises a sequence that comprises at least 14, 15, 16, 17, 18, 19, or 20 nucleotides of the sequence of any of SEQ ID NOs: 1-21, the first repressor domain comprises a moiety chosen from DNMT1, DNM T3a/3L, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1 and the second domain comprises a moiety chosen from DNMT1, DNMt3a/3L, MQ1, KRAB, G9A, HDAC8, LSD1, EZH2, or FOG1.
50. The expression repressor of any of embodiments 35-49, wherein the CRISPR/Cas molecule comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof.
51. The expression repressor of any of embodiments 35-50, wherein the CRISPR/Cas molecule comprises a catalytically inactive CRISPR/Cas protein, e.g., dCas9.
52. The expression repressor of any of the preceding embodiments, which: (i) comprises one or more nuclear localization signal sequences (NLS), or (ii) does not comprise an NLS.
53. The expression repressor of any of the preceding embodiments, comprising a first NLS at the N
terminus, e.g., wherein the first NLS has a sequence of SEQ ID NO: 86.
54. The expression repressor of any of the preceding embodiments, comprising an NLS, e.g., a second NLS, at the C terminus, e.g., having a sequence of SEQ ID NO: 87.
13 55. The expression repressor of any of the preceding embodiments, wherein the first and the second NLS
have the same sequence.
56. The expression repressor of any of embodiments 52-55, wherein the first and the second NLS have different sequences.
57. The expression repressor of any of the preceding embodiments, which comprises an epitope tag.
58. The expression repressor of embodiment 57, wherein the epitope tag is an HA tag, e.g., an HA tag comprising an amnio acid seq of SEQ ID NO: 80.
59. The expression repressor of any of embodiments 37-58, wherein the anchor sequence comprises the sequence of SEQ ID NO: 81 or 82, or a sequence with no more than 8, 7, 6, 5, 4, 3, 2, or 1 alteration relative thereto.
60. The expression repressor of any of embodiments 37-58, wherein the anchor sequence comprises a sequence according to SEQ ID NO: 83 or 84, or a sequence with no more than 8, 7, 6, 5, 4, 3, 2, or 1 alteration relative thereto.
61. The expression repressor of any of embodiments 37-60, wherein the anchor sequence is on the same chromosome as the gene.
62. The expression repressor of any of embodiments 37-61, wherein the anchor sequence is upstream of the target gene (e.g., upstream of the TSS or upstream of the promoter).
63. The expression repressor of any of embodiments 37-61 wherein the anchor sequence is downstream of the target gene (e.g., downstream of the TSS or upstream of the promoter).
64. The expression repressor of any of the preceding embodiments, wherein binding of the expression repressor to the target gene locus decreases expression of the target gene in a cell by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression of the target gene in the absence of the expression repressor.
have the same sequence.
56. The expression repressor of any of embodiments 52-55, wherein the first and the second NLS have different sequences.
57. The expression repressor of any of the preceding embodiments, which comprises an epitope tag.
58. The expression repressor of embodiment 57, wherein the epitope tag is an HA tag, e.g., an HA tag comprising an amnio acid seq of SEQ ID NO: 80.
59. The expression repressor of any of embodiments 37-58, wherein the anchor sequence comprises the sequence of SEQ ID NO: 81 or 82, or a sequence with no more than 8, 7, 6, 5, 4, 3, 2, or 1 alteration relative thereto.
60. The expression repressor of any of embodiments 37-58, wherein the anchor sequence comprises a sequence according to SEQ ID NO: 83 or 84, or a sequence with no more than 8, 7, 6, 5, 4, 3, 2, or 1 alteration relative thereto.
61. The expression repressor of any of embodiments 37-60, wherein the anchor sequence is on the same chromosome as the gene.
62. The expression repressor of any of embodiments 37-61, wherein the anchor sequence is upstream of the target gene (e.g., upstream of the TSS or upstream of the promoter).
63. The expression repressor of any of embodiments 37-61 wherein the anchor sequence is downstream of the target gene (e.g., downstream of the TSS or upstream of the promoter).
64. The expression repressor of any of the preceding embodiments, wherein binding of the expression repressor to the target gene locus decreases expression of the target gene in a cell by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression of the target gene in the absence of the expression repressor.
14 65. The expression repressor of any of the preceding embodiments, wherein binding of the expression repressor to the target gene locus appreciably decreases expression of the target gene for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22 days.
66. The expression repressor of any of embodiments 18-65, wherein the first and/or second repressor domain comprises a DNA methyltransferase, a histone methyltransferase, a histone deacetylase, a histone demethylase, or a recruiter of a histone modifying complex.
67. An expression repression system comprising:
a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain, wherein the first DNA-targeting moiety specifically binds a first DNA
sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence, and wherein the first repressor domain is different from the second repressor domain.
68. An expression repression system comprising:
a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain, wherein the first or second repressor domain comprises an MQ1 domain or functional variant or fragment thereof, wherein the first repressor domain is different from the second repressor domain.
69. An expression repression system of embodiment 67 or 68, wherein the first expression repressor further comprises a third repressor domain.
70. An expression repression system of any of embodiments 67-69, wherein the second expression repressor further comprises a fourth repressor domain.
71. The expression repression system of any of embodiments 67-70, wherein the first DNA-targeting moiety specifically binds a first DNA sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence.
5 72. The expression repression system of any of embodiments 67-70, wherein the first DNA-targeting moiety and the second DNA-targeting moiety specifically bind the same DNA
sequence.
73. The expression repression system of any of embodiments 67-72, wherein the first DNA-targeting moiety comprises a first CRISPR/Cas molecule comprising a 10 first CRISPR/Cas protein and first guide RNA, and the second DNA-targeting moiety comprises a second CRISPR/Cas molecule comprising a second CRISPR/Cas protein and a second guide RNA.
74. The expression repression system of embodiment 73, wherein the first CRISPR/Cas protein does not bind the second guide RNA, e.g., binds with a KD
66. The expression repressor of any of embodiments 18-65, wherein the first and/or second repressor domain comprises a DNA methyltransferase, a histone methyltransferase, a histone deacetylase, a histone demethylase, or a recruiter of a histone modifying complex.
67. An expression repression system comprising:
a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain, wherein the first DNA-targeting moiety specifically binds a first DNA
sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence, and wherein the first repressor domain is different from the second repressor domain.
68. An expression repression system comprising:
a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain, wherein the first or second repressor domain comprises an MQ1 domain or functional variant or fragment thereof, wherein the first repressor domain is different from the second repressor domain.
69. An expression repression system of embodiment 67 or 68, wherein the first expression repressor further comprises a third repressor domain.
70. An expression repression system of any of embodiments 67-69, wherein the second expression repressor further comprises a fourth repressor domain.
71. The expression repression system of any of embodiments 67-70, wherein the first DNA-targeting moiety specifically binds a first DNA sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence.
5 72. The expression repression system of any of embodiments 67-70, wherein the first DNA-targeting moiety and the second DNA-targeting moiety specifically bind the same DNA
sequence.
73. The expression repression system of any of embodiments 67-72, wherein the first DNA-targeting moiety comprises a first CRISPR/Cas molecule comprising a 10 first CRISPR/Cas protein and first guide RNA, and the second DNA-targeting moiety comprises a second CRISPR/Cas molecule comprising a second CRISPR/Cas protein and a second guide RNA.
74. The expression repression system of embodiment 73, wherein the first CRISPR/Cas protein does not bind the second guide RNA, e.g., binds with a KD
15 of at least 10, 20, 50, 100, 1000, or 10,000 nM, and the second CRISPR/Cas protein does not bind the first guide RNA, e.g., binds with a KD of at least 10, 20, 50, 100, 1000, or 10,000 nM.
75. The expression repression system of either of embodiments 73 or 74:
wherein the first CRISPR/Cas protein comprises a different amino acid sequence than the second CRISPR/Cas protein.
76. The expression repression system of any of embodiments 67-75, wherein the first or second DNA-targeting moiety comprises a CRISPR/Cas molecule comprising a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof.
77. The expression repression system of any of embodiments 67-76, wherein the first DNA-targeting moiety comprises a first CRISPR/Cas molecule comprising a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof, and the second DNA-targeting moiety comprises a second CRISPR/Cas molecule comprising a different Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof.
78. The expression repression system of any of embodiments 70-77, wherein the first, second, third, or fourth repressor domain comprises a histone methyltransferase activity.
75. The expression repression system of either of embodiments 73 or 74:
wherein the first CRISPR/Cas protein comprises a different amino acid sequence than the second CRISPR/Cas protein.
76. The expression repression system of any of embodiments 67-75, wherein the first or second DNA-targeting moiety comprises a CRISPR/Cas molecule comprising a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof.
77. The expression repression system of any of embodiments 67-76, wherein the first DNA-targeting moiety comprises a first CRISPR/Cas molecule comprising a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof, and the second DNA-targeting moiety comprises a second CRISPR/Cas molecule comprising a different Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof.
78. The expression repression system of any of embodiments 70-77, wherein the first, second, third, or fourth repressor domain comprises a histone methyltransferase activity.
16 79. The expression repression system of embodiment 70-78, wherein the first, second, third, or fourth repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, or a functional variant or fragment of any thereof, e.g., a SET domain of any thereof.
80. The expression repression system of any of embodiments 70-79, wherein the first, second, third, or fourth repressor domain comprises a histone demethylase activity (e.g., a lysine demethylase activity).
81. The expression repression system of any of embodiment 70-80, wherein the first, second, third, or fourth repressor domain comprises a protein chosen from KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066 (or a functional variant or fragment of any thereof.
82. The expression repression system of any of embodiments 70-81, wherein the first, second, third, or fourth repressor domain comprises a histone deacetylase activity.
83. The expression repression system of embodiment 70-82, wherein the first, second, third, or fourth repressor domain comprises a protein chosen from HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof.
84. The expression repression system of any of embodiments 70-83, wherein the first, second, second, third, or fourth repressor domain comprises a DNA methyltransferase activity.
85. The expression repression system of embodiment 70-84, wherein the first, second, third, or fourth repressor domain comprises a protein chosen from MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, or a functional variant or fragment of any thereof.
86. The expression repression system of any of embodiments 70-85, wherein the first, second, third, or fourth repressor domain comprises a transcription repressor activity.
80. The expression repression system of any of embodiments 70-79, wherein the first, second, third, or fourth repressor domain comprises a histone demethylase activity (e.g., a lysine demethylase activity).
81. The expression repression system of any of embodiment 70-80, wherein the first, second, third, or fourth repressor domain comprises a protein chosen from KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066 (or a functional variant or fragment of any thereof.
82. The expression repression system of any of embodiments 70-81, wherein the first, second, third, or fourth repressor domain comprises a histone deacetylase activity.
83. The expression repression system of embodiment 70-82, wherein the first, second, third, or fourth repressor domain comprises a protein chosen from HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof.
84. The expression repression system of any of embodiments 70-83, wherein the first, second, second, third, or fourth repressor domain comprises a DNA methyltransferase activity.
85. The expression repression system of embodiment 70-84, wherein the first, second, third, or fourth repressor domain comprises a protein chosen from MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, or a functional variant or fragment of any thereof.
86. The expression repression system of any of embodiments 70-85, wherein the first, second, third, or fourth repressor domain comprises a transcription repressor activity.
17 87. The expression repression system of embodiment 70-86, wherein the first, second, third, or fourth repressor domain comprises a protein chosen from KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment of any thereof.
.. 88. The expression repression system of any of embodiments 67-87, wherein:
the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, .. EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, .. DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
89. The expression repression system of any of embodiments 69-88, wherein:
(i) the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and (ii) the third repressor domain comprises a protein (e.g., a different protein) chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B,
.. 88. The expression repression system of any of embodiments 67-87, wherein:
the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, .. EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, .. DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
89. The expression repression system of any of embodiments 69-88, wherein:
(i) the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and (ii) the third repressor domain comprises a protein (e.g., a different protein) chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B,
18 KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
90. The expression repression system of any of embodiments 69-89, wherein:
(i) the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, (ii) the second repressor domain comprises a protein (e.g., different protein) chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and (iii) the third repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, .. DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
91. The expression repression system of any of embodiments 70-90, wherein:
90. The expression repression system of any of embodiments 69-89, wherein:
(i) the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, (ii) the second repressor domain comprises a protein (e.g., different protein) chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and (iii) the third repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, .. DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
91. The expression repression system of any of embodiments 70-90, wherein:
19 (i) the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, (ii) the second repressor domain comprises a protein (e.g., a different protein) chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, (iii) the third repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof and (iv) the fourth repressor domain comprises a protein (e.g., a different protein) chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
92. The expression repression system of any of embodiments 70-91, wherein:
(i) the second repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, 5 HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and (ii) the fourth repressor domain comprises a protein (e.g., a different protein) chosen from 10 SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, 15 DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
93. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a histone
92. The expression repression system of any of embodiments 70-91, wherein:
(i) the second repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, 5 HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and (ii) the fourth repressor domain comprises a protein (e.g., a different protein) chosen from 10 SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, 15 DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
93. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a histone
20 demethylase activity.
94. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a histone deacetylase activity.
95. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a DNA
methyltransferase activity.
96. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a DNA
demethylase activity.
94. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a histone deacetylase activity.
95. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a DNA
methyltransferase activity.
96. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a DNA
demethylase activity.
21 97. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a transcription repressor activity.
98. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a histone methyltransferase activity (e.g., wherein the histone methyltransferase activities are the same or different from each other).
99. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a histone deacetylase activity.
100. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a DNA
methyltransferase activity.
101. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a DNA demethylase activity.
102. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a transcription repressor activity.
103. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a histone demethylase activity (e.g., wherein the histone demethylase activities are the same or different from each other).
104. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a DNA
methyltransferase activity.
98. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a histone methyltransferase activity (e.g., wherein the histone methyltransferase activities are the same or different from each other).
99. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a histone deacetylase activity.
100. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a DNA
methyltransferase activity.
101. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a DNA demethylase activity.
102. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a transcription repressor activity.
103. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a histone demethylase activity (e.g., wherein the histone demethylase activities are the same or different from each other).
104. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a DNA
methyltransferase activity.
22 105. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a DNA demethylase activity.
106. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a transcription repressor activity.
107. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a histone deacetylase activity (e.g., wherein the histone deacetylase activities are the same or different from each other).
108. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a DNA
demethylase activity.
109. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a transcription repressor activity.
110. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a DNA
methyltransferase activity (e.g., wherein the DNA methyltransferase activities are the same or different from each other).
111. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA demethylase activity and the second repressor domain comprises a transcription repressor activity.
112. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA demethylase activity and the second repressor domain comprises a DNA demethylase activity (e.g., wherein the DNA demethylase activities are the same or different from each other).
106. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a transcription repressor activity.
107. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a histone deacetylase activity (e.g., wherein the histone deacetylase activities are the same or different from each other).
108. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a DNA
demethylase activity.
109. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a transcription repressor activity.
110. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a DNA
methyltransferase activity (e.g., wherein the DNA methyltransferase activities are the same or different from each other).
111. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA demethylase activity and the second repressor domain comprises a transcription repressor activity.
112. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a DNA demethylase activity and the second repressor domain comprises a DNA demethylase activity (e.g., wherein the DNA demethylase activities are the same or different from each other).
23 113. The expression repression system of any of embodiments 67-92, wherein the first repressor domain comprises a transcription repressor activity and the second repressor domain comprises a transcription repressor activity (e.g., wherein the transcription repressor activities are the same or different from each other).
114. The expression repression system of any of embodiments 67-113, wherein:
the first repressor domain comprises a protein selected from: KRAB, a SET
domain (e.g., the SET domain of SETDB1, EZH2, G9A, or SUV39H1), histone demethylase LSD1, FOG1 (e.g., the N-terminal residues of FOG1), KAP1, or a functional variant or fragment of any thereof; and the second repressor domain comprises a protein selected from: DNMT3A (e.g., human DNMT3A), DNMT3B, DNMT3L, DNMT3A/3L complex, bacterial MQ1, or a functional variant or fragment of any thereof.
115. The expression repression system of any of embodiments 67-114, wherein:
the first repressor domain comprises a protein selected from: EZH2, G9A, SUV39H1, FOG1, or a functional variant or fragment of any thereof; and the second repressor domain comprises a protein selected from: DNMT3A (e.g., human DNMT3A), DNMT3B, DNMT3L, DNMT3A/3L, DNMT1, LSD1, FOG1, KRAB, HDAC8, bacterial MQ1, or a functional variant or fragment of any thereof.
116. The expression repression system of any of embodiments67-115, wherein:
(i) the first repressor domain comprises a protein selected from: KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KAP1, HDAC8, MQ1, DNMT1, DNMT3A, DNMT3a/31, or a functional variant or fragment of any thereof;
and (ii) the second repressor domain comprises a protein selected from: KRAB, FOG1, G9A, LSD1, HDAC8, EZH2, DNMT3a/31, MQ1, DNMT1, DBMT3A, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L or a functional variant or fragment of any thereof.
117. The expression repression system of any of embodiments 67-116, wherein the first repressor domain comprises KRAB, and the second repressor domain comprises DNMT3A (e.g., human DNMT3A).
114. The expression repression system of any of embodiments 67-113, wherein:
the first repressor domain comprises a protein selected from: KRAB, a SET
domain (e.g., the SET domain of SETDB1, EZH2, G9A, or SUV39H1), histone demethylase LSD1, FOG1 (e.g., the N-terminal residues of FOG1), KAP1, or a functional variant or fragment of any thereof; and the second repressor domain comprises a protein selected from: DNMT3A (e.g., human DNMT3A), DNMT3B, DNMT3L, DNMT3A/3L complex, bacterial MQ1, or a functional variant or fragment of any thereof.
115. The expression repression system of any of embodiments 67-114, wherein:
the first repressor domain comprises a protein selected from: EZH2, G9A, SUV39H1, FOG1, or a functional variant or fragment of any thereof; and the second repressor domain comprises a protein selected from: DNMT3A (e.g., human DNMT3A), DNMT3B, DNMT3L, DNMT3A/3L, DNMT1, LSD1, FOG1, KRAB, HDAC8, bacterial MQ1, or a functional variant or fragment of any thereof.
116. The expression repression system of any of embodiments67-115, wherein:
(i) the first repressor domain comprises a protein selected from: KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KAP1, HDAC8, MQ1, DNMT1, DNMT3A, DNMT3a/31, or a functional variant or fragment of any thereof;
and (ii) the second repressor domain comprises a protein selected from: KRAB, FOG1, G9A, LSD1, HDAC8, EZH2, DNMT3a/31, MQ1, DNMT1, DBMT3A, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L or a functional variant or fragment of any thereof.
117. The expression repression system of any of embodiments 67-116, wherein the first repressor domain comprises KRAB, and the second repressor domain comprises DNMT3A (e.g., human DNMT3A).
24 118. The expression repression system of any of embodiments 67-115, wherein the first repressor domain comprises G9A or a functional variant or fragment thereof, and the second repressor domain comprises KRAB or a functional variant or fragment thereof.
119. The expression repression system of any of embodiments 67-118, wherein the first repressor domain comprises LSD1 or a functional variant or fragment thereof and the second repressor domain comprises KRAB or a functional variant or fragment thereof.
120. The expression repression system of any of embodiments 67-119, wherein the first repressor domain comprises KRAB or a functional variant or fragment thereof and the second repressor domain comprises bacterial MQ1 or a functional variant or fragment thereof.
121. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety; and the second repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the second repressor domain is N-terminal of the second DNA-targeting moiety.
122. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain of comprises LSD1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the second repressor domain is N-terminal of the second DNA-targeting moiety.
123. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises MQ1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
124. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises LSD1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
125. The expression repression system of any of embodiments 67-120, wherein:
5 the first repressor domain comprises LSD1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the second repressor domain is N-terminal of the second DNA-targeting moiety.
10 126. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety; and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
127. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety; and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, .. wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
128. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises MQ1 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
.. 129. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises MQ1 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety 130. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
131. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises FOG1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises FOG1 or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
132. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises LSD1 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
133. The expression repression system of any of embodiments 69-130, wherein:
the first repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises LSD1 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
134. The expression repression system of any of embodiments 67-133, wherein the first DNA-targeting moiety is selected from a TAL effector molecule, a CRISPR/Cas molecule, a zinc finger domain, a tetR
domain, a meganuclease, or an oligonucleotide.
135. The expression repression system of any of embodiments 67-134, wherein the second DNA-targeting moiety is selected from a TAL effector molecule, a CRISPR/Cas molecule, a zinc finger domain, a tetR domain, a meganuclease, or an oligonucleotide.
136. The expression repression system of any of embodiments67-135, wherein the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein (e.g., that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof) and a guide RNA.
137. The expression repression system of any of embodiments67-136, wherein the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein (e.g., that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof) and a guide RNA.
138. The expression repression system of any of embodiments67-137, wherein:
the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof), and the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a different CRISPR/Cas protein that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof).
139. The expression repression system of any of embodiments 135-138, wherein:
the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule encoded by a nucleic acid sequence of SEQ ID NO: 45 or 89, and the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule encoded by a nucleic acid sequence of SEQ ID NO: 45 or 89.
140. The expression repression system of any of embodiments 135-138, wherein:
the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising an amino acid sequence of SEQ ID NO: 46 or 88, and the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising an amino acid sequence of SEQ ID NO: 46 or 88.
141. The expression repression system of any of embodiments 67-140, wherein the first or the second DNA-targeting moiety specifically binds to a promoter sequence or to DNA
proximal to a promoter sequence, e.g., a promoter sequence operably linked to a target gene.
142. The expression repression system of any of embodiments 67-141, wherein the first or the second DNA-targeting moiety specifically binds to an enhancer sequence or to DNA
proximal to an enhancer sequence, e.g., an enhancer sequence that affects expression of and/or is operably linked to a target gene.
143. The expression repression system of any of embodiments 67-142, wherein the first or the second DNA targeting moiety specifically binds to a target gene, e.g., sequence comprised between the transcription start site and stop codon of a target gene.
144. The expression repression system of any of embodiments 67-143, wherein the first or the second DNA targeting moiety specifically binds to the transcription start site of a target gene.
145. The expression repression system of any of embodiments 67-144, wherein the first or the second DNA targeting moiety specifically binds to an intron, e.g., a splice site associated with an intron, of a target gene.
146. The expression repression system of any of embodiments 67-145, wherein the first or the second DNA targeting moiety specifically binds to an exon of a target gene.
147. The expression repression system of any of embodiments 67-146, wherein first or the second DNA
targeting moiety specifically binds to an anchor sequence.
148. The expression repression system of embodiment 147, wherein the anchor sequence comprises the sequence of SEQ ID NO: 81 or 82, or a sequence with no more than 8, 7, 6, 5, 4, 3, 2, or 1 alterations relative thereto.
149. The expression repression system of embodiment 147, wherein the anchor sequence comprises the sequence of SEQ ID NO: 83 or 84, or a sequence with no more than 8, 7, 6, 5, 4, 3, 2, or 1 alterations relative thereto.
150. The expression repression system of any of embodiments 147-149, wherein the anchor sequence is on the same chromosome as the gene.
151. The expression repression system of any of embodiments 147-149, wherein the anchor sequence is on a different chromosome as the gene.
152. The expression repression system of any of embodiments 147-151, wherein the anchor sequence is upstream of the target gene (e.g., upstream of the TSS or upstream of the promoter).
153. The expression repression system of any of embodiments 148-151, wherein the anchor sequence is downstream of the target gene (e.g., downstream of the TSS or upstream of the promoter).
154. The expression repression system of any of embodiments 67-141, wherein the first DNA sequence is a first site within or proximal to a promoter operably linked to a target gene and the second DNA
sequence is a second site within or proximal to the promoter operably linked to the target gene.
155. The expression repression system of any of embodiments 67-142, wherein the first DNA sequence is within or proximal to a promoter operably linked to a target gene and the second DNA sequence is a within or proximal to an enhancer that affects expression of or is operably linked to the target gene.
156. The expression repression system of any of embodiments 67-142, wherein the first DNA sequence is within or proximal to an enhancer that affects expression of or is operably linked to a target gene and the second DNA sequence is a within or proximal to a promoter operably linked to the target gene.
157. The expression repression system of any of embodiments 67-141 or 143, wherein the first DNA
sequence comprises the transcription start site of a target gene and the second DNA sequence is within or proximal to a promoter operably linked to the target gene.
158. The expression repression system of any of embodiments 67-140, 142 or 143, wherein the first DNA
sequence comprises the transcription start site of a target gene and the second DNA sequence is within or proximal to an enhancer that affects expression of or is operably linked to the target gene.
5 159. The expression repression system of any of embodiments 67-141 or 143, wherein the first DNA
sequence within or proximal to a promoter operably linked to a target gene and the second DNA sequence is the transcription start site of the target gene.
160. The expression repression system of any of embodiments 67-140, 142, or 143, wherein the first 10 DNA sequence is within or proximal to an enhancer that affects expression of or is operably linked to a target gene and the second DNA sequence is the transcription start site of the target gene.
161. The expression repression system of any of embodiments 67-160, wherein the distance between the first DNA sequence and the second DNA sequence is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 15 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 base pairs or any size therebetween, e.g., 20-500 base pairs.
20 .. 162.The expression repression system of any of embodiments 67-161, wherein the distance between the first DNA sequence and the second DNA sequence is no more than 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 base pairs or any size therebetween, e.g., 20-500 base pairs.
163. The expression repression system of any of embodiments 67-162, wherein the first DNA sequence and the second DNA sequence are situated on different nucleic acids, e.g., different chromosomes.
164. The expression repression system of any of embodiments 67-163, wherein the target gene is 13-2-microglobulin, MYC, HSPA1B, GATA1, APOB, FOXP3, CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL6, CXCL7, and/or CXCL8.
165. The expression repression system of any of embodiments 73-164, wherein the first guide RNA
comprises a sequence specific to, e.g., complementary to, the first DNA
sequence and the second guide RNA comprises a sequence specific to, e.g., complementary to, the second DNA
sequence.
166. The expression repression system of any of embodiments 67-165, wherein the first DNA-targeting moiety does not appreciably bind to the second DNA sequence and the second DNA-targeting moiety does not appreciably bind to the first DNA sequence.
167. The expression repression system of any of embodiments 67-166, wherein the first DNA-targeting moiety binds the second DNA sequence with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or 100,000 nM, and the second DNA-targeting moiety binds the first DNA
sequence with binds with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or 100,000 nM.
168. The expression repression system of any of embodiments 67-167, wherein binding of the first expression repressor to the first DNA sequence decreases expression of a target gene, e.g., a target gene operably linked to the first DNA sequence, in a cell.
169. The expression repression system of embodiment 168, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the first expression repressor, e.g., as measured by ELISA or as described in Examples 2-4.
170. The expression repression system of any of embodiments 67-169, wherein binding of the first expression repressor to the first DNA sequence appreciably decreases expression of a target gene, e.g., a target gene operably linked to the first DNA sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1,2, 3, 4, 5, 6,7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
171. The expression repression system of any of embodiments 67-170, wherein binding of the second expression repressor to the second DNA sequence decreases expression of a target gene, e.g., a target gene operably linked to the second DNA sequence, in a cell.
172. The expression repression system of embodiment 171, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the second expression repressor, e.g., as measured by ELISA or as described in Examples 2-4.
173. The expression repression system of any of embodiments 67-172, wherein binding of the second expression repressor to the second DNA sequence appreciably decreases expression of a target gene, e.g., a target gene operably linked to the second DNA sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
174. The expression repression system of any of embodiments 67-173, wherein binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA
.. sequence decreases expression of a target gene, e.g., a target gene operably linked to the first DNA
sequence and/or the second DNA sequence, in a cell.
175. The expression repression system of embodiment 174, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the first and second expression repressors, e.g., as measured by ELISA or as described in Examples 2-4.
176. The expression repression system of any of embodiments 1-175, wherein binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA
sequence appreciably decreases expression of a target gene, e.g., a target gene operably linked to the first DNA sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 hours, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
177. The expression repression system of any of embodiments 174-176, wherein the decrease in .. expression resulting from the binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA sequence is greater than the decrease in expression resulting from the binding of the first expression repressor to the first DNA
sequence or the binding of the second expression repressor to the second DNA sequence individually.
178. The expression repression system of embodiment 177, wherein the binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA sequence decreases expression 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 50x, or 100x more than either the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually, e.g., as measured by ELISA or as described in Examples 2-4.
179. The expression repression system of embodiment 174-178, wherein the decrease in expression resulting from the binding of the first expression repressor to the first DNA
sequence and the second expression repressor to the second DNA sequence persists for a longer time (e.g., more hours, days, or cell divisions) than the decrease in expression resulting from the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually.
180. The expression repression system of embodiment 179, wherein the binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA sequence decreases expression 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 50x, or 100x longer (e.g., as measured in hours, days, or cell divisions) than either the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually, e.g., as measured by ELISA or as described in Examples 2-4.
181. The expression repression system of any of embodiments 174-180, wherein the cell is dividing at a rate of no more than once per 24, 36, 48, 60, or 72 hours (and optionally, at least once per 24, 36, 48, 60, or 72 hours).
182. The expression repression system of any of embodiments 174-181, wherein the cell is dividing at a rate of at least once per 24, 36, 48, 60, or 72 hours (and optionally, no more than once per 24, 36, 48, 60, or 72 hours).
183. The expression repression system of any of embodiments 174-182, wherein expression is appreciably decreased indefinitely (e.g., for a time period greater than can be experimentally measured).
184. The expression repression system of any of embodiments 67-183, wherein the first DNA-targeting moiety comprises an S. pyo genes CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. pyogenes dCas9, e.g., a dCas9 comprising an amino acid sequence of SEQ ID NO: 46; or a S. aureus CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. aureus dCas9, e.g., a dCas9 comprising an amino acid sequence of SEQ
ID NO: 88 and the second DNA-targeting moiety comprises an S. pyogenes CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. pyogenes dCas9, e.g., a dCas9 comprising an amino acid sequence of SEQ ID NO: 46; or a CRISPR/Cas molecule comprising a S.
aureus CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S.
aureus dCas9, e.g., a dCas9 comprising an amino acid sequence of SEQ ID NO: 88.
185. The expression repression system of any of embodiments 67-184, wherein the first DNA-targeting moiety comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein, e.g., a catalytically inactive dCas9, e.g., a dCas9, e.g., a dCas9 comprising one or more mutations selected from D 10A, D839A, H840A, and N863A mutations; and the second DNA-targeting moiety comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein, e.g., a catalytically inactive dCas9, e.g., a dCas9, e.g., a dCas9 comprising one or more mutations selected from DlOA, D839A, H840A, and N863A mutations.
186. The expression repression system of any of embodiments 67-185, wherein the first expression repressor comprises a first DNA-targeting moiety comprising a CRISPR/Cas molecule comprising an S. pyogenes CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. pyogenes dCas9, and a first repressor domain comprising KRAB or a functional variant or fragment thereof; and the second expression repressor comprises a second DNA-targeting moiety comprising a CRISPR/Cas molecule comprising a S. aureus CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. aureus dCas9, and a second repressor domain comprising bacterial MQ1 or a functional variant or fragment thereof.
187. A polypeptide comprising:
a DNA-targeting moiety; and a repressor domain comprising bacterial MQ1 or a functional variant or fragment thereof.
188. A polypeptide comprising:
a DNA-targeting moiety;
a first repressor domain comprising MQ1, DNMT1, DNMT3a/31, KRAB, G9A, HDAC8, LSD1, EZH2, FOG1 or a functional variant or fragment thereof, and a second repressor domain comprising MQ1, DNMT1, DNMT3a/31, KRAB, G9A, HDAC8, LSD1, EZH2, FOG1 or a functional variant or fragment thereof.
189. The polypeptide of embodiment 88a, wherein the DNA-targeting moiety is situated between the first 5 repressor domain and the second repressor domain.
190. A nucleic acid encoding the first expression repressor of the expression repression system of any of embodiments 67-189.
10 191. A nucleic acid encoding the second expression repressor of the expression repression system of any of embodiments 67-189.
192. A nucleic acid encoding the expression repression system, e.g., the first expression repressor and the second expression repressor, of any of embodiments 67-189.
193. The nucleic acid of any of embodiments 190-192, which is an RNA, e.g., an mRNA.
194. An expression repression system comprising:
a first nucleic acid comprising a sequence encoding the first expression repressor of the expression repression system of any of embodiments 67-189; and a second nucleic acid comprising a sequence encoding the second expression repressor of the expression repression system of any of embodiments 67-189.
195. A nucleic acid encoding the polypeptide of any of embodiments 187-189.
196. The nucleic acid any of embodiments 190-193 or 195, wherein the nucleic acid comprises mRNA.
197. A vector comprising the nucleic acid encoding the expression repressor or the expression repression system of any preceding embodiment.
198. A lipid nanoparticle comprising the expression repressor, expression repression system, nucleic acid, mRNA, or vector of any of the preceding embodiments.
199. The lipid nanoparticle of embodiment 198 comprising an ionizable lipid, e.g., a cationic lipid, e.g., MC3, SSOP.
200. The lipid nanoparticle of embodiment 198 or 199, further comprising one or more of neutral lipids, ionizable amine-containing lipids, biodegradable alkyn lipids, steroids, phospholipids, polyunsaturated lipids, structural lipids (e.g., sterols), PEG, cholesterol, or polymer conjugated lipids.
201. A reaction mixture comprising the expression repressor, expression repression system, nucleic acid, vector, or lipid nanoparticle of any of the preceding embodiments.
202. The reaction mixture of embodiment 201 further comprising a cell.
203. A cell comprising the expression repressor, expression repression system, nucleic acid, mRNA, vector, or nanoparticle of any of embodiments 1-202.
204. The cell of embodiment 203, wherein the cell is selected from a hepatocyte, a neuronal cell, an endothelial cell, a myocyte, and a lymphocyte.
205. A cell produced by a method comprising:
providing i) a cell and ii) an expression repressor, expression repression system, polypeptide, nucleic acid, vector, lipid nanoparticle, or reaction mixture of any of embodiments 1-202; and contacting the cell with the expression repressor, expression repression system, polypeptide, nucleic acid, vector, lipid nanoparticle, or reaction mixture.
206. A pharmaceutical composition comprising the expression repressor, expression repression system, nucleic acid, polypeptide, vector, lipid nanoparticle, or reaction mixture of any of embodiments 1-202, and at least one pharmaceutically acceptable excipient or carrier.
207. The expression repression system of any of embodiments 1-202, wherein the first expression repressor further comprises an additional moiety selected from: a tagging or monitoring moiety, a cleavable moiety (e.g., a cleavable moiety positioned between a DNA-targeting moiety and a repressor domain or at the N- or C-terminal end of a polypeptide), a small molecule, a membrane translocating polypeptide, or a pharmacoagent moiety.
208. The expression repression system of any of embodiments 1-202, wherein the second expression repressor further comprises an additional moiety selected from: a tagging or monitoring moiety, a cleavable moiety (e.g., a cleavable moiety positioned between a DNA-targeting moiety and a repressor domain or at the N- or C-terminal end of a polypeptide), a small molecule, a membrane translocating polypeptide, or a pharmacoagent moiety.
209. A method of decreasing expression of a target genomic sequence in a cell, comprising:
providing the expression repressor, expression repression system, nucleic acid, polypeptide, vector, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition of any of embodiments 1-204 or 206; and contacting the cell with the expression repressor, expression repression system, nucleic acid, polypeptide, vector, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition thereby decreasing expression of the target genomic sequence.
210. A method of epigenetically modifying a target genomic sequence in a cell, comprising:
providing an expression repressor, expression repression system, nucleic acid, vector, polypeptide, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition of any of embodiments 1-204 or 206; and contacting the cell with the expression repressor, expression repression system, nucleic acid, .. vector, polypeptide, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition, thereby epigenetically modifying the target genomic sequence.
211. The method off embodiment 209 or 210, wherein the target genomic sequence is:
a target gene (e.g., a site within a target gene, e.g., an exon, intron, or splice site), e.g., the gene encoding I3-2-microglobulin (I32M), the gene encoding MYC, the gene encoding HSPA1B, or the gene encoding GATA1, a transcription control element operably linked to a target gene, or an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to the target gene.
212. The method of embodiment211, wherein the epigenetic modification results in a decrease in expression of the target gene.
213. The expression repressor, expression repression system, cell, pharmaceutical composition, nucleic acid, vector, or method of any of embodiments174-212, wherein the cell is a mammalian cell, e.g., a human cell.
214. The method of embodiment213, wherein the method is performed ex vivo (e.g., in a sample from a subject) or in vivo (e.g., by administering the expression repression system to a subject).
215. The method of any of embodiments209-214, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in an otherwise similar cell in the absence of the expression repression system.
216. The method of any of embodiments209-214, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in an otherwise similar cell that has not been contacted with the expression repression system.
217. The method of any of embodiments209-216, wherein expression is decreased for a time period of at least 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
218. The method of any of embodiments215-217, wherein the decrease in expression comprises a decrease in the level of RNA, e.g., mRNA, encoded by the target gene.
219. The method of any of embodiments215-218, wherein the decrease in expression comprises a decrease in the level of a protein encoded by the target gene.
220. The method of any of embodiments 209-219, wherein providing comprises contacting the cell with a nucleic acid, e.g., vector, encoding the expression repression system to the cell comprising the target gene.
221. The method of any of embodiments 209-220, wherein the nucleic acid encoding the first expression repressor is disposed on a first nucleic acid molecule and the nucleic acid encoding the second expression repressor is disposed on a second nucleic acid molecule.
222. The method of any of embodiments 209-221, wherein the nucleic acid encoding the first expression repressor is disposed on the same nucleic acid molecule as the nucleic acid encoding the second expression repressor.
223. The method of any of embodiments 209-222, wherein administering comprises contacting the cell with the first nucleic acid molecule and the second nucleic acid molecule together, e.g., simultaneously.
224. The method of any of embodiments 209-223, wherein administering comprises contacting the cell with the first nucleic acid molecule and the second nucleic acid molecule separately, e.g., sequentially.
225. The method of any of embodiments 209-224, wherein providing comprises contacting the cell with a vesicle comprising the expression repression system or a nucleic acid, e.g., vector, encoding the expression repression system.
226. The method of any of embodiments 209-225, wherein providing comprises contacting the cell with a lipid nanoparticle (LNP) comprising the expression repression system or a nucleic acid, e.g., vector, encoding the expression repression system.
227. The method of any of embodiments 209-226, wherein the expression repression system comprises a first expression repressor and a second expression repressor, and providing comprises administering the first expression repressor and, separately, administering the second expression repressor.
228. The method of any of embodiments 209-227, wherein the expression repression system comprises a first expression repressor and a second expression repressor and providing comprises administering the first expression repressor and the second expression repressor together, e.g., simultaneously.
229. A method of treating a condition associated with over-expression of a target gene in a subject, comprising:
administering the expression repressor, expression repression system, nucleic acid, vector, polypeptide, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition of any of embodiments 1-204 or 206-208 to the subject, thereby treating a condition associated with over-expression of a target gene in a subject.
230. A method of treating a condition associated with mis-regulation of a target gene in a subject, comprising:
administering the expression repressor, expression repression system, nucleic acid, vector, polypeptide, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition of any of 5 embodiments 1-204 or 206-208 to the subject, thereby treating a condition associated with mis-regulation of a target gene in a subject.
231. A method of making a cell comprising an expression repression system, the method comprising:
providing the expression repressor, expression repression system, nucleic acid, vector, 10 polypeptide, or pharmaceutical composition of any of embodiments 1-201 or 206-208; and contacting the cell with the expression repressor, expression repression system, nucleic acid, vector, or pharmaceutical composition, thereby making a cell comprising an expression repression system.
15 232. A method of making a nucleic acid encoding an expression repression system, the method comprising:
providing a first nucleic acid encoding the first expression repressor of the expression repression system of any of embodiments 67-186, 207, or 208;
providing a second nucleic acid encoding the second expression repressor of the expression 20 repression system of any of embodiments 67-186, 9207, or 208;
combining (e.g., ligating or recombining) the first nucleic acid and the second nucleic acid into a single nucleic acid molecule, thereby making a nucleic acid encoding an expression repression system.
119. The expression repression system of any of embodiments 67-118, wherein the first repressor domain comprises LSD1 or a functional variant or fragment thereof and the second repressor domain comprises KRAB or a functional variant or fragment thereof.
120. The expression repression system of any of embodiments 67-119, wherein the first repressor domain comprises KRAB or a functional variant or fragment thereof and the second repressor domain comprises bacterial MQ1 or a functional variant or fragment thereof.
121. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety; and the second repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the second repressor domain is N-terminal of the second DNA-targeting moiety.
122. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain of comprises LSD1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the second repressor domain is N-terminal of the second DNA-targeting moiety.
123. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises MQ1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
124. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises LSD1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
125. The expression repression system of any of embodiments 67-120, wherein:
5 the first repressor domain comprises LSD1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the second repressor domain is N-terminal of the second DNA-targeting moiety.
10 126. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety; and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
127. The expression repression system of any of embodiments 67-120, wherein:
the first repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety; and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, .. wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
128. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety; and the second repressor domain comprises MQ1 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
.. 129. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises MQ1 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety 130. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises HDAC8 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
131. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises FOG1 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises FOG1 or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
132. The expression repression system of any of embodiments 69-120, wherein:
the first repressor domain comprises EZH2 or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises LSD1 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
133. The expression repression system of any of embodiments 69-130, wherein:
the first repressor domain comprises G9A or a functional variant or fragment thereof, wherein optionally the first repressor domain is N-terminal of the first DNA-targeting moiety, the third repressor domain comprises KRAB or a functional variant or fragment thereof, wherein optionally the third repressor domain is C-terminal of the first DNA-targeting moiety, and the second repressor domain comprises LSD1 or a functional variant or fragment thereof, wherein optionally the second repressor domain is C-terminal of the second DNA-targeting moiety.
134. The expression repression system of any of embodiments 67-133, wherein the first DNA-targeting moiety is selected from a TAL effector molecule, a CRISPR/Cas molecule, a zinc finger domain, a tetR
domain, a meganuclease, or an oligonucleotide.
135. The expression repression system of any of embodiments 67-134, wherein the second DNA-targeting moiety is selected from a TAL effector molecule, a CRISPR/Cas molecule, a zinc finger domain, a tetR domain, a meganuclease, or an oligonucleotide.
136. The expression repression system of any of embodiments67-135, wherein the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein (e.g., that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof) and a guide RNA.
137. The expression repression system of any of embodiments67-136, wherein the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein (e.g., that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof) and a guide RNA.
138. The expression repression system of any of embodiments67-137, wherein:
the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof), and the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a different CRISPR/Cas protein that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof).
139. The expression repression system of any of embodiments 135-138, wherein:
the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule encoded by a nucleic acid sequence of SEQ ID NO: 45 or 89, and the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule encoded by a nucleic acid sequence of SEQ ID NO: 45 or 89.
140. The expression repression system of any of embodiments 135-138, wherein:
the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising an amino acid sequence of SEQ ID NO: 46 or 88, and the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising an amino acid sequence of SEQ ID NO: 46 or 88.
141. The expression repression system of any of embodiments 67-140, wherein the first or the second DNA-targeting moiety specifically binds to a promoter sequence or to DNA
proximal to a promoter sequence, e.g., a promoter sequence operably linked to a target gene.
142. The expression repression system of any of embodiments 67-141, wherein the first or the second DNA-targeting moiety specifically binds to an enhancer sequence or to DNA
proximal to an enhancer sequence, e.g., an enhancer sequence that affects expression of and/or is operably linked to a target gene.
143. The expression repression system of any of embodiments 67-142, wherein the first or the second DNA targeting moiety specifically binds to a target gene, e.g., sequence comprised between the transcription start site and stop codon of a target gene.
144. The expression repression system of any of embodiments 67-143, wherein the first or the second DNA targeting moiety specifically binds to the transcription start site of a target gene.
145. The expression repression system of any of embodiments 67-144, wherein the first or the second DNA targeting moiety specifically binds to an intron, e.g., a splice site associated with an intron, of a target gene.
146. The expression repression system of any of embodiments 67-145, wherein the first or the second DNA targeting moiety specifically binds to an exon of a target gene.
147. The expression repression system of any of embodiments 67-146, wherein first or the second DNA
targeting moiety specifically binds to an anchor sequence.
148. The expression repression system of embodiment 147, wherein the anchor sequence comprises the sequence of SEQ ID NO: 81 or 82, or a sequence with no more than 8, 7, 6, 5, 4, 3, 2, or 1 alterations relative thereto.
149. The expression repression system of embodiment 147, wherein the anchor sequence comprises the sequence of SEQ ID NO: 83 or 84, or a sequence with no more than 8, 7, 6, 5, 4, 3, 2, or 1 alterations relative thereto.
150. The expression repression system of any of embodiments 147-149, wherein the anchor sequence is on the same chromosome as the gene.
151. The expression repression system of any of embodiments 147-149, wherein the anchor sequence is on a different chromosome as the gene.
152. The expression repression system of any of embodiments 147-151, wherein the anchor sequence is upstream of the target gene (e.g., upstream of the TSS or upstream of the promoter).
153. The expression repression system of any of embodiments 148-151, wherein the anchor sequence is downstream of the target gene (e.g., downstream of the TSS or upstream of the promoter).
154. The expression repression system of any of embodiments 67-141, wherein the first DNA sequence is a first site within or proximal to a promoter operably linked to a target gene and the second DNA
sequence is a second site within or proximal to the promoter operably linked to the target gene.
155. The expression repression system of any of embodiments 67-142, wherein the first DNA sequence is within or proximal to a promoter operably linked to a target gene and the second DNA sequence is a within or proximal to an enhancer that affects expression of or is operably linked to the target gene.
156. The expression repression system of any of embodiments 67-142, wherein the first DNA sequence is within or proximal to an enhancer that affects expression of or is operably linked to a target gene and the second DNA sequence is a within or proximal to a promoter operably linked to the target gene.
157. The expression repression system of any of embodiments 67-141 or 143, wherein the first DNA
sequence comprises the transcription start site of a target gene and the second DNA sequence is within or proximal to a promoter operably linked to the target gene.
158. The expression repression system of any of embodiments 67-140, 142 or 143, wherein the first DNA
sequence comprises the transcription start site of a target gene and the second DNA sequence is within or proximal to an enhancer that affects expression of or is operably linked to the target gene.
5 159. The expression repression system of any of embodiments 67-141 or 143, wherein the first DNA
sequence within or proximal to a promoter operably linked to a target gene and the second DNA sequence is the transcription start site of the target gene.
160. The expression repression system of any of embodiments 67-140, 142, or 143, wherein the first 10 DNA sequence is within or proximal to an enhancer that affects expression of or is operably linked to a target gene and the second DNA sequence is the transcription start site of the target gene.
161. The expression repression system of any of embodiments 67-160, wherein the distance between the first DNA sequence and the second DNA sequence is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 15 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 base pairs or any size therebetween, e.g., 20-500 base pairs.
20 .. 162.The expression repression system of any of embodiments 67-161, wherein the distance between the first DNA sequence and the second DNA sequence is no more than 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 base pairs or any size therebetween, e.g., 20-500 base pairs.
163. The expression repression system of any of embodiments 67-162, wherein the first DNA sequence and the second DNA sequence are situated on different nucleic acids, e.g., different chromosomes.
164. The expression repression system of any of embodiments 67-163, wherein the target gene is 13-2-microglobulin, MYC, HSPA1B, GATA1, APOB, FOXP3, CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL6, CXCL7, and/or CXCL8.
165. The expression repression system of any of embodiments 73-164, wherein the first guide RNA
comprises a sequence specific to, e.g., complementary to, the first DNA
sequence and the second guide RNA comprises a sequence specific to, e.g., complementary to, the second DNA
sequence.
166. The expression repression system of any of embodiments 67-165, wherein the first DNA-targeting moiety does not appreciably bind to the second DNA sequence and the second DNA-targeting moiety does not appreciably bind to the first DNA sequence.
167. The expression repression system of any of embodiments 67-166, wherein the first DNA-targeting moiety binds the second DNA sequence with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or 100,000 nM, and the second DNA-targeting moiety binds the first DNA
sequence with binds with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or 100,000 nM.
168. The expression repression system of any of embodiments 67-167, wherein binding of the first expression repressor to the first DNA sequence decreases expression of a target gene, e.g., a target gene operably linked to the first DNA sequence, in a cell.
169. The expression repression system of embodiment 168, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the first expression repressor, e.g., as measured by ELISA or as described in Examples 2-4.
170. The expression repression system of any of embodiments 67-169, wherein binding of the first expression repressor to the first DNA sequence appreciably decreases expression of a target gene, e.g., a target gene operably linked to the first DNA sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1,2, 3, 4, 5, 6,7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
171. The expression repression system of any of embodiments 67-170, wherein binding of the second expression repressor to the second DNA sequence decreases expression of a target gene, e.g., a target gene operably linked to the second DNA sequence, in a cell.
172. The expression repression system of embodiment 171, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the second expression repressor, e.g., as measured by ELISA or as described in Examples 2-4.
173. The expression repression system of any of embodiments 67-172, wherein binding of the second expression repressor to the second DNA sequence appreciably decreases expression of a target gene, e.g., a target gene operably linked to the second DNA sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
174. The expression repression system of any of embodiments 67-173, wherein binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA
.. sequence decreases expression of a target gene, e.g., a target gene operably linked to the first DNA
sequence and/or the second DNA sequence, in a cell.
175. The expression repression system of embodiment 174, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the first and second expression repressors, e.g., as measured by ELISA or as described in Examples 2-4.
176. The expression repression system of any of embodiments 1-175, wherein binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA
sequence appreciably decreases expression of a target gene, e.g., a target gene operably linked to the first DNA sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 hours, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
177. The expression repression system of any of embodiments 174-176, wherein the decrease in .. expression resulting from the binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA sequence is greater than the decrease in expression resulting from the binding of the first expression repressor to the first DNA
sequence or the binding of the second expression repressor to the second DNA sequence individually.
178. The expression repression system of embodiment 177, wherein the binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA sequence decreases expression 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 50x, or 100x more than either the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually, e.g., as measured by ELISA or as described in Examples 2-4.
179. The expression repression system of embodiment 174-178, wherein the decrease in expression resulting from the binding of the first expression repressor to the first DNA
sequence and the second expression repressor to the second DNA sequence persists for a longer time (e.g., more hours, days, or cell divisions) than the decrease in expression resulting from the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually.
180. The expression repression system of embodiment 179, wherein the binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA sequence decreases expression 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 50x, or 100x longer (e.g., as measured in hours, days, or cell divisions) than either the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually, e.g., as measured by ELISA or as described in Examples 2-4.
181. The expression repression system of any of embodiments 174-180, wherein the cell is dividing at a rate of no more than once per 24, 36, 48, 60, or 72 hours (and optionally, at least once per 24, 36, 48, 60, or 72 hours).
182. The expression repression system of any of embodiments 174-181, wherein the cell is dividing at a rate of at least once per 24, 36, 48, 60, or 72 hours (and optionally, no more than once per 24, 36, 48, 60, or 72 hours).
183. The expression repression system of any of embodiments 174-182, wherein expression is appreciably decreased indefinitely (e.g., for a time period greater than can be experimentally measured).
184. The expression repression system of any of embodiments 67-183, wherein the first DNA-targeting moiety comprises an S. pyo genes CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. pyogenes dCas9, e.g., a dCas9 comprising an amino acid sequence of SEQ ID NO: 46; or a S. aureus CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. aureus dCas9, e.g., a dCas9 comprising an amino acid sequence of SEQ
ID NO: 88 and the second DNA-targeting moiety comprises an S. pyogenes CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. pyogenes dCas9, e.g., a dCas9 comprising an amino acid sequence of SEQ ID NO: 46; or a CRISPR/Cas molecule comprising a S.
aureus CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S.
aureus dCas9, e.g., a dCas9 comprising an amino acid sequence of SEQ ID NO: 88.
185. The expression repression system of any of embodiments 67-184, wherein the first DNA-targeting moiety comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein, e.g., a catalytically inactive dCas9, e.g., a dCas9, e.g., a dCas9 comprising one or more mutations selected from D 10A, D839A, H840A, and N863A mutations; and the second DNA-targeting moiety comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein, e.g., a catalytically inactive dCas9, e.g., a dCas9, e.g., a dCas9 comprising one or more mutations selected from DlOA, D839A, H840A, and N863A mutations.
186. The expression repression system of any of embodiments 67-185, wherein the first expression repressor comprises a first DNA-targeting moiety comprising a CRISPR/Cas molecule comprising an S. pyogenes CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. pyogenes dCas9, and a first repressor domain comprising KRAB or a functional variant or fragment thereof; and the second expression repressor comprises a second DNA-targeting moiety comprising a CRISPR/Cas molecule comprising a S. aureus CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. aureus dCas9, and a second repressor domain comprising bacterial MQ1 or a functional variant or fragment thereof.
187. A polypeptide comprising:
a DNA-targeting moiety; and a repressor domain comprising bacterial MQ1 or a functional variant or fragment thereof.
188. A polypeptide comprising:
a DNA-targeting moiety;
a first repressor domain comprising MQ1, DNMT1, DNMT3a/31, KRAB, G9A, HDAC8, LSD1, EZH2, FOG1 or a functional variant or fragment thereof, and a second repressor domain comprising MQ1, DNMT1, DNMT3a/31, KRAB, G9A, HDAC8, LSD1, EZH2, FOG1 or a functional variant or fragment thereof.
189. The polypeptide of embodiment 88a, wherein the DNA-targeting moiety is situated between the first 5 repressor domain and the second repressor domain.
190. A nucleic acid encoding the first expression repressor of the expression repression system of any of embodiments 67-189.
10 191. A nucleic acid encoding the second expression repressor of the expression repression system of any of embodiments 67-189.
192. A nucleic acid encoding the expression repression system, e.g., the first expression repressor and the second expression repressor, of any of embodiments 67-189.
193. The nucleic acid of any of embodiments 190-192, which is an RNA, e.g., an mRNA.
194. An expression repression system comprising:
a first nucleic acid comprising a sequence encoding the first expression repressor of the expression repression system of any of embodiments 67-189; and a second nucleic acid comprising a sequence encoding the second expression repressor of the expression repression system of any of embodiments 67-189.
195. A nucleic acid encoding the polypeptide of any of embodiments 187-189.
196. The nucleic acid any of embodiments 190-193 or 195, wherein the nucleic acid comprises mRNA.
197. A vector comprising the nucleic acid encoding the expression repressor or the expression repression system of any preceding embodiment.
198. A lipid nanoparticle comprising the expression repressor, expression repression system, nucleic acid, mRNA, or vector of any of the preceding embodiments.
199. The lipid nanoparticle of embodiment 198 comprising an ionizable lipid, e.g., a cationic lipid, e.g., MC3, SSOP.
200. The lipid nanoparticle of embodiment 198 or 199, further comprising one or more of neutral lipids, ionizable amine-containing lipids, biodegradable alkyn lipids, steroids, phospholipids, polyunsaturated lipids, structural lipids (e.g., sterols), PEG, cholesterol, or polymer conjugated lipids.
201. A reaction mixture comprising the expression repressor, expression repression system, nucleic acid, vector, or lipid nanoparticle of any of the preceding embodiments.
202. The reaction mixture of embodiment 201 further comprising a cell.
203. A cell comprising the expression repressor, expression repression system, nucleic acid, mRNA, vector, or nanoparticle of any of embodiments 1-202.
204. The cell of embodiment 203, wherein the cell is selected from a hepatocyte, a neuronal cell, an endothelial cell, a myocyte, and a lymphocyte.
205. A cell produced by a method comprising:
providing i) a cell and ii) an expression repressor, expression repression system, polypeptide, nucleic acid, vector, lipid nanoparticle, or reaction mixture of any of embodiments 1-202; and contacting the cell with the expression repressor, expression repression system, polypeptide, nucleic acid, vector, lipid nanoparticle, or reaction mixture.
206. A pharmaceutical composition comprising the expression repressor, expression repression system, nucleic acid, polypeptide, vector, lipid nanoparticle, or reaction mixture of any of embodiments 1-202, and at least one pharmaceutically acceptable excipient or carrier.
207. The expression repression system of any of embodiments 1-202, wherein the first expression repressor further comprises an additional moiety selected from: a tagging or monitoring moiety, a cleavable moiety (e.g., a cleavable moiety positioned between a DNA-targeting moiety and a repressor domain or at the N- or C-terminal end of a polypeptide), a small molecule, a membrane translocating polypeptide, or a pharmacoagent moiety.
208. The expression repression system of any of embodiments 1-202, wherein the second expression repressor further comprises an additional moiety selected from: a tagging or monitoring moiety, a cleavable moiety (e.g., a cleavable moiety positioned between a DNA-targeting moiety and a repressor domain or at the N- or C-terminal end of a polypeptide), a small molecule, a membrane translocating polypeptide, or a pharmacoagent moiety.
209. A method of decreasing expression of a target genomic sequence in a cell, comprising:
providing the expression repressor, expression repression system, nucleic acid, polypeptide, vector, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition of any of embodiments 1-204 or 206; and contacting the cell with the expression repressor, expression repression system, nucleic acid, polypeptide, vector, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition thereby decreasing expression of the target genomic sequence.
210. A method of epigenetically modifying a target genomic sequence in a cell, comprising:
providing an expression repressor, expression repression system, nucleic acid, vector, polypeptide, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition of any of embodiments 1-204 or 206; and contacting the cell with the expression repressor, expression repression system, nucleic acid, .. vector, polypeptide, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition, thereby epigenetically modifying the target genomic sequence.
211. The method off embodiment 209 or 210, wherein the target genomic sequence is:
a target gene (e.g., a site within a target gene, e.g., an exon, intron, or splice site), e.g., the gene encoding I3-2-microglobulin (I32M), the gene encoding MYC, the gene encoding HSPA1B, or the gene encoding GATA1, a transcription control element operably linked to a target gene, or an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to the target gene.
212. The method of embodiment211, wherein the epigenetic modification results in a decrease in expression of the target gene.
213. The expression repressor, expression repression system, cell, pharmaceutical composition, nucleic acid, vector, or method of any of embodiments174-212, wherein the cell is a mammalian cell, e.g., a human cell.
214. The method of embodiment213, wherein the method is performed ex vivo (e.g., in a sample from a subject) or in vivo (e.g., by administering the expression repression system to a subject).
215. The method of any of embodiments209-214, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in an otherwise similar cell in the absence of the expression repression system.
216. The method of any of embodiments209-214, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in an otherwise similar cell that has not been contacted with the expression repression system.
217. The method of any of embodiments209-216, wherein expression is decreased for a time period of at least 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
218. The method of any of embodiments215-217, wherein the decrease in expression comprises a decrease in the level of RNA, e.g., mRNA, encoded by the target gene.
219. The method of any of embodiments215-218, wherein the decrease in expression comprises a decrease in the level of a protein encoded by the target gene.
220. The method of any of embodiments 209-219, wherein providing comprises contacting the cell with a nucleic acid, e.g., vector, encoding the expression repression system to the cell comprising the target gene.
221. The method of any of embodiments 209-220, wherein the nucleic acid encoding the first expression repressor is disposed on a first nucleic acid molecule and the nucleic acid encoding the second expression repressor is disposed on a second nucleic acid molecule.
222. The method of any of embodiments 209-221, wherein the nucleic acid encoding the first expression repressor is disposed on the same nucleic acid molecule as the nucleic acid encoding the second expression repressor.
223. The method of any of embodiments 209-222, wherein administering comprises contacting the cell with the first nucleic acid molecule and the second nucleic acid molecule together, e.g., simultaneously.
224. The method of any of embodiments 209-223, wherein administering comprises contacting the cell with the first nucleic acid molecule and the second nucleic acid molecule separately, e.g., sequentially.
225. The method of any of embodiments 209-224, wherein providing comprises contacting the cell with a vesicle comprising the expression repression system or a nucleic acid, e.g., vector, encoding the expression repression system.
226. The method of any of embodiments 209-225, wherein providing comprises contacting the cell with a lipid nanoparticle (LNP) comprising the expression repression system or a nucleic acid, e.g., vector, encoding the expression repression system.
227. The method of any of embodiments 209-226, wherein the expression repression system comprises a first expression repressor and a second expression repressor, and providing comprises administering the first expression repressor and, separately, administering the second expression repressor.
228. The method of any of embodiments 209-227, wherein the expression repression system comprises a first expression repressor and a second expression repressor and providing comprises administering the first expression repressor and the second expression repressor together, e.g., simultaneously.
229. A method of treating a condition associated with over-expression of a target gene in a subject, comprising:
administering the expression repressor, expression repression system, nucleic acid, vector, polypeptide, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition of any of embodiments 1-204 or 206-208 to the subject, thereby treating a condition associated with over-expression of a target gene in a subject.
230. A method of treating a condition associated with mis-regulation of a target gene in a subject, comprising:
administering the expression repressor, expression repression system, nucleic acid, vector, polypeptide, lipid nanoparticle, reaction mixture, cell, or pharmaceutical composition of any of 5 embodiments 1-204 or 206-208 to the subject, thereby treating a condition associated with mis-regulation of a target gene in a subject.
231. A method of making a cell comprising an expression repression system, the method comprising:
providing the expression repressor, expression repression system, nucleic acid, vector, 10 polypeptide, or pharmaceutical composition of any of embodiments 1-201 or 206-208; and contacting the cell with the expression repressor, expression repression system, nucleic acid, vector, or pharmaceutical composition, thereby making a cell comprising an expression repression system.
15 232. A method of making a nucleic acid encoding an expression repression system, the method comprising:
providing a first nucleic acid encoding the first expression repressor of the expression repression system of any of embodiments 67-186, 207, or 208;
providing a second nucleic acid encoding the second expression repressor of the expression 20 repression system of any of embodiments 67-186, 9207, or 208;
combining (e.g., ligating or recombining) the first nucleic acid and the second nucleic acid into a single nucleic acid molecule, thereby making a nucleic acid encoding an expression repression system.
25 233. A cell treated or produced by the method of any of embodiments 209-228 or 230.
234. The cell of embodiment 233, wherein the cell does not comprise the expression repression system, e.g., at least one component (e.g., all components) of the expression repression system.
30 235. The cell of either of embodiments 233 or 234, wherein expression of the target gene is decreased relative to a cell not contacted, treated, or produced by the method of any of embodiments 209-228 or 230.
236. A kit comprising a container comprising a composition comprising an expression repressor, an expression repression system, one or more nucleic acids encoding said expression repressor or an expression repression system, a vector, a lipid nanoparticle, or a pharmaceutical composition of any of embodiments 1-208 and a set of instructions comprising at least one method for modulating, e.g., .. decreasing the expression of a gene within a cell with said composition.
DEFINITIONS
Anchor Sequence: The term "anchor sequence" as used herein, refers to a nucleic acid sequence recognized by a nucleating agent that binds sufficiently to form an anchor sequence-mediated conjunction, e.g., a complex. In some embodiments, an anchor sequence comprises one or more CTCF
binding motifs. In some embodiments, an anchor sequence is not located within a gene coding region. In some embodiments, an anchor sequence is located within an intergenic region.
In some embodiments, an anchor sequence is not located within either of an enhancer or a promoter. In some embodiments, an anchor sequence is located at least 400 bp, at least 450 bp, at least 500 bp, at least 550 bp, at least 600 bp, at least 650 bp, at least 700 bp, at least 750 bp, at least 800 bp, at least 850 bp, at least 900 bp, at least 950 bp, or at least lkb away from any transcription start site. In some embodiments, an anchor sequence is located within a region that is not associated with genomic imprinting, monoallelic expression, and/or monoallelic epigenetic marks. In some embodiments, the anchor sequence has one or more functions selected from binding an endogenous nucleating polypeptide (e.g., CTCF), interacting with a second anchor sequence to form an anchor sequence mediated conjunction, or insulating against an enhancer that is outside the anchor sequence mediated conjunction. In some embodiments of the present disclosure, technologies are provided that may specifically target a particular anchor sequence or anchor sequences, without targeting other anchor sequences (e.g., sequences that may contain a nucleating agent (e.g., CTCF) binding motif in a different context); such targeted anchor sequences may be referred to as the "target anchor sequence". In some embodiments, sequence and/or activity of a target anchor sequence is modulated while sequence and/or activity of one or more other anchor sequences that may be present in the same system (e.g., in the same cell and/or in some embodiments on the same nucleic acid molecule ¨
e.g., the same chromosome) as the targeted anchor sequence is not modulated.
In some embodiments, the anchor sequence comprises or is a nucleating polypeptide binding motif. In some embodiments, the anchor sequence is adjacent to a nucleating polypeptide binding motif.
Anchor Sequence-Mediated Conjunction: The term "anchor sequence-mediated conjunction" as used herein, refers to a DNA structure, in some cases, a complex, that occurs and/or is maintained via physical interaction or binding of at least two anchor sequences in the DNA by one or more polypeptides, such as nucleating polypeptides, or one or more proteins and/or a nucleic acid entity (such as RNA or DNA), that bind the anchor sequences to enable spatial proximity and functional linkage between the anchor sequences (see, e.g. Figure 1).
Associated with: Two events or entities are "associated" with one another, as that term is used herein, if presence, level, form and/or function of one is correlated with that of the other. For example, in some embodiments, a particular entity (e.g., polypeptide, genetic signature, metabolite, microbe, etc.) is considered to be associated with a particular disease, disorder, or condition, if its presence, level, form and/or function correlates with incidence of and/or susceptibility to the disease, disorder, or condition (e.g., across a relevant population). In some embodiments, two or more entities are physically "associated" with one another if they interact, directly or indirectly, so that they are and/or remain in physical proximity with one another. In some embodiments, two or more entities that are physically associated with one another are covalently linked to one another; in some embodiments, two or more entities that are physically associated with one another are not covalently linked to one another but are .. non-covalently associated, for example by means of hydrogen bonds, van der Waals interaction, hydrophobic interactions, magnetism, and combinations thereof. In some embodiments, a DNA sequence is "associated with" a target genomic or transcription complex when the nucleic acid is at least partially within the target genomic or transcription complex, and expression of a gene in the DNA sequence is affected by formation or disruption of the target genomic or transcription complex.
Domain: As used herein, the term "domain" refers to a section or portion of an entity. In some embodiments, a "domain" is associated with a particular structural and/or functional feature of the entity so that, when the domain is physically separated from the rest of its parent entity, it substantially or entirely retains the particular structural and/or functional feature.
Alternatively, or additionally, in some embodiments, a domain may be or include a portion of an entity that, when separated from that (parent) entity and linked with a different (recipient) entity, substantially retains and/or imparts on the recipient entity one or more structural and/or functional features that characterized it in the parent entity. In some embodiments, a domain is or comprises a section or portion of a molecule (e.g., a small molecule, carbohydrate, lipid, nucleic acid, polypeptide, etc.). In some embodiments, a domain is or comprises a section of a polypeptide. In some such embodiments, a domain is characterized by a particular structural element (e.g., a particular amino acid sequence or sequence motif, alpha-helix character, beta-sheet character, coiled-coil character, random coil character, etc.), and/or by a particular functional feature (e.g., binding activity, enzymatic activity, folding activity, signaling activity, etc.).
Expression repressor: As used herein, the term "expression repressor" refers to an agent or entity with one or more functionalities that decrease expression of a target gene in a cell and that specifically binds to a DNA sequence (e.g., a DNA sequence associated with a target gene, or a transcription control element operably linked to a target gene). An expression repressor comprises at least one DNA-targeting moiety and at least one repressor domain.
Expression repression system: As used herein, the term "expression repression system" refers to a plurality of expression repressors which decrease expression of a target gene in a cell. In some embodiments, an expression repression system comprises a first expression repressor and a second expression repressor, wherein the first expression repressor and second expression repressor (or nucleic acids encoding the first expression repressor and second expression repressor) are present together in a single composition, mixture, or pharmaceutical composition. In some embodiments, an expression repression system comprises a first expression repressor and a second expression repressor, wherein the first expression repressor and second expression repressor (or nucleic acids encoding the first expression repressor and second expression repressor) are present in separate compositions or pharmaceutical compositions. In some embodiments, the first expression repressor and the second expression repressor are present in the same cell at the same time. In some embodiments, the first expression repressor and the second expression repressor are not present in the same cell at the same time, e.g., they are present sequentially. For example, the first expression repressor may be present in a cell for a first time period, and then the second expression repressor may be present in the cell for a second time period, wherein the first and second time periods may be overlapping or non-overlapping.
Genomic complex: As used herein, the term "genomic complex" is a complex that brings .. together two genomic sequence elements that are spaced apart from one another on one or more chromosomes, via interactions between and among a plurality of protein and/or other components (potentially including, the genomic sequence elements). In some embodiments, the genomic sequence elements are anchor sequences to which one or more protein components of the complex binds. In some embodiments, a genomic complex may comprise an anchor sequence-mediated conjunction. In some embodiments, a genomic sequence element may be or comprise a CTCF binding motif, a promoter and/or an enhancer. In some embodiments, a genomic sequence element includes at least one or both of a promoter and/or regulatory site (e.g., an enhancer). In some embodiments, complex formation is nucleated at the genomic sequence element(s) and/or by binding of one or more of the protein component(s) to the genomic sequence element(s). As will be understood by those skilled in the art, in some embodiments, co-localization (e.g., conjunction) of the genomic sites via formation of the complex alters DNA topology at or near the genomic sequence element(s), including, in some embodiments, between them. In some embodiments, a genomic complex comprises an anchor sequence-mediated conjunction, which comprises one or more loops. In some embodiments, a genomic complex as described herein is nucleated by a nucleating polypeptide such as, for example, CTCF
and/or Cohesin. In some embodiments, a genomic complex as described herein may include, for example, one or more of CTCF, Cohesin, non-coding RNA (e.g., eRNA), transcriptional machinery proteins (e.g., RNA polymerase, one or more transcription factors, for example selected from the group consisting of TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIH, etc.), transcriptional regulators (e.g., Mediator, P300, enhancer-binding proteins, repressor-binding proteins, histone modifiers, etc.), etc. In some embodiments, a genomic complex as described herein includes one or more polypeptide components and/or one or more nucleic acid components (e.g., one or more RNA components), which may, in some embodiments, be interacting with one another and/or with one or more genomic sequence elements (e.g., anchor sequences, promoter sequences, regulatory sequences (e.g., enhancer sequences)) so as to constrain a stretch of genomic DNA
into a topological configuration (e.g., a loop) that it does not adopt when the complex is not formed.
Nucleic acid: As used herein, in its broadest sense, the term "nucleic acid"
refers to any compound and/or substance that is or can be incorporated into an oligonucleotide chain. In some embodiments, a nucleic acid is a compound and/or substance that is or can be incorporated into an oligonucleotide chain via a phosphodiester linkage. As will be clear from context, in some embodiments, "nucleic acid" refers to an individual nucleic acid residue (e.g., a nucleotide and/or nucleoside); in some embodiments, "nucleic acid" refers to an oligonucleotide chain comprising individual nucleic acid residues. In some embodiments, a "nucleic acid" is or comprises RNA; in some embodiments, a "nucleic acid" is or comprises DNA. In some embodiments, a nucleic acid is, comprises, or consists of one or more natural nucleic acid residues. In some embodiments, a nucleic acid is, comprises, or consists of one or more nucleic acid analogs. In some embodiments, a nucleic acid analog differs from a nucleic acid in that it does not utilize a phosphodiester backbone. For example, in some embodiments, a nucleic acid is, comprises, or consists of one or more "peptide nucleic acids", which are known in the art and have peptide bonds instead of phosphodiester bonds in the backbone, are considered within the scope of the present disclosure. Alternatively, or additionally, in some embodiments, a nucleic acid has one or more phosphorothioate and/or 5'-N-phosphoramidite linkages rather than phosphodiester bonds. In some embodiments, a nucleic acid is, comprises, or consists of one or more natural nucleosides (e.g., adenosine, thymidine, guanosine, cytidine, uridine, deoxyadenosine, deoxythymidine, deoxy guanosine, and deoxycytidine). In some embodiments, a nucleic acid is, comprises, or consists of one or more nucleoside analogs (e.g., 2-aminoadenosine, 2-thiothymidine, inosine, pyrrolo-pyrimidine, 3 -methyl adenosine, 5-methylcytidine, C-5 propynyl-cytidine, C-5 propynyl-uridine, 2-aminoadenosine, C5-bromouridine, C5-fluorouridine, C5-iodouridine, C5-propynyl-uridine, C5 -propynyl-cytidine, C5-methylcytidine, 2-aminoadenosine, 7-deazaadenosine, 7-deazaguanosine, 8-oxoadenosine, 8-oxoguanosine, 0(6)-methylguanine, 2-thiocytidine, methylated bases, intercalated bases, and combinations thereof). In some embodiments, a nucleic acid comprises one or more modified sugars (e.g., 2'-fluororibose, ribose, 2'-deoxyribose, arabinose, and hexose) as compared with those in natural nucleic acids. In some embodiments, a nucleic acid has a nucleotide sequence that encodes a functional gene product such as an RNA or protein. In some embodiments, a nucleic acid includes one or more introns. In some embodiments, nucleic acids are prepared by one or more of isolation from a natural source, enzymatic 5 synthesis by polymerization based on a complementary template (in vivo or in vitro), reproduction in a recombinant cell or system, and chemical synthesis. In some embodiments, a nucleic acid is at least 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 20, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 600, 700, 800, 900, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000 or more residues long. In some 10 embodiments, a nucleic acid is partly or wholly single stranded; in some embodiments, a nucleic acid is partly or wholly double stranded. In some embodiments a nucleic acid has a nucleotide sequence comprising at least one element that encodes, or is the complement of a sequence that encodes, a polypeptide. In some embodiments, a nucleic acid has enzymatic activity.
Operably linked: As used herein, the phrase "operably linked" refers to a juxtaposition wherein 15 the components described are in a relationship permitting them to function in their intended manner. A
transcription control element "operably linked" to a functional element, e.g., gene, is associated in such a way that expression and/or activity of the functional element, e.g., gene, is achieved under conditions compatible with the transcription control element. In some embodiments, "operably linked" transcription control elements are contiguous (e.g., covalently linked) with coding elements, e.g., genes, of interest; in 20 some embodiments, operably linked transcription control elements act in trans to or otherwise at a distance from the functional element, e.g., gene, of interest. In some embodiments, operably linked means two nucleic acid sequences are comprised on the same nucleic acid molecule. In a further embodiment, operably linked may further mean that the two nucleic acid sequences are proximal to one another on the same nucleic acid molecule, e.g., within 1000, 500, 100, 50, or 10 base pairs of each other 25 or directly adjacent to each other.
Peptide, Polyp eptide, Protein: As used herein, the terms "peptide,"
"polypeptide," and "protein"
refer to a compound comprised of amino acid residues covalently linked by peptide bonds, or by means other than peptide bonds. A protein or peptide must contain at least two amino acids, and no limitation is 30 placed on the maximum number of amino acids that can comprise a protein's or peptide's sequence.
Polypeptides include any peptide or protein comprising two or more amino acids joined to each other by peptide bonds or by means other than peptide bonds. As used herein, the term refers to both short chains, which also commonly are referred to in the art as peptides, oligopeptides, and oligomers, for example, and to longer chains, which generally are referred to in the art as proteins, of which there are many types.
Repressor domain: As used herein, the term "repressor domain" refers to a domain with one or more functionalities that decrease expression of a target gene in a cell when appropriately localized in the nucleus of a cell. In some embodiments, a repressor domain is or comprises a polypeptide. In some embodiments, a repressor domain is or comprises a polypeptide and a nucleic acid. A functionality associated with a repressor domain may directly affect expression of a target gene, e.g., blocking recruitment of a transcription factor that would stimulate expression of the gene. A functionality associated with a repressor domain may indirectly affect expression of a target gene, e.g., introducing epigenetic modifications or recruiting other factors that introduce epigenetic modifications that induce a change in chromosomal topology that inhibits expression of a target gene.
Specific binding: As used herein, the term "specific binding" refers to an ability to discriminate between possible binding partners in the environment in which binding is to occur. In some embodiments, a binding agent that interacts with one particular target when other potential targets are present is said to "bind specifically" to the target with which it interacts.
In some embodiments, specific binding is assessed by detecting or determining degree of association between the binding agent and its .. partner; in some embodiments, specific binding is assessed by detecting or determining degree of dissociation of a binding agent-partner complex. In some embodiments, specific binding is assessed by detecting or determining ability of the binding agent to compete with an alternative interaction between its partner and another entity. In some embodiments, specific binding is assessed by performing such detections or determinations across a range of concentrations.
Substantially: As used herein, the term "substantially" refers to the qualitative condition of exhibiting total or near-total extent or degree of a characteristic or property of interest. One of ordinary skill in the art will understand that biological and chemical phenomena rarely, if ever, go to completion and/or proceed to completeness or achieve or avoid an absolute result. The term "substantially" may therefore be used in some embodiments herein to capture potential lack of completeness inherent in many biological and chemical phenomena.
Symptoms are reduced: As used herein, the phrase "symptoms are reduced" may be used when one or more symptoms of a particular disease, disorder or condition is reduced in magnitude (e.g., intensity, severity, etc.) and/or frequency. In some embodiments, a delay in the onset of a particular symptom is considered one form of reducing the frequency of that symptom.
Target: An agent or entity is considered to "target" another agent or entity, in accordance with the present disclosure, if it binds specifically to the targeted agent or entity under conditions in which they come into contact with one another. In some embodiments, for example, an antibody (or antigen-binding fragment thereof) targets its cognate epitope or antigen. In some embodiments, a nucleic acid having a particular sequence targets a nucleic acid of substantially complementary sequence.
Target gene: As used herein, the term "target gene" means a gene that is targeted for modulation, e.g., of expression. In some embodiments, a target gene is part of a targeted genomic complex (e.g., a gene that has at least part of its genomic sequence as part of a target genomic complex, e.g. inside an anchor sequence-mediated conjunction), which genomic complex is targeted by one or more modulating agents as described herein. In some embodiments, modulation comprises inhibition of expression of the target gene. In some embodiments, a target gene is modulated by contacting the target gene or a transcription control element operably linked to the target gene with an expression repression system, e.g., expression repressor(s), described herein. In some embodiments, a target gene is aberrantly expressed (e.g., over-expressed) in a cell, e.g., a cell in a subject (e.g., patient).
DNA-targeting moiety: As used herein, in the context of expression repressors, the term "DNA-targeting moiety" means a domain that specifically binds to a DNA sequence (e.g., a DNA sequence associated with a target gene, or a transcription control element operably linked to a target gene).
Therapeutic agent: As used herein, the phrase "therapeutic agent" refers to an agent that, when administered to a subject, has a therapeutic effect and/or elicits a desired biological and/or pharmacological effect. In some embodiments, a therapeutic agent is any substance that can be used to alleviate, ameliorate, relieve, inhibit, prevent, delay onset of, reduce severity of, and/or reduce incidence of one or more symptoms or features of a disease, disorder, and/or condition.
In some embodiments, a therapeutic agent comprises an expression repression system, e.g., an expression repressor, described herein. In some embodiments, a therapeutic agent comprises a nucleic acid encoding an expression repression system, e.g., an expression repressor, described herein. In some embodiments, a therapeutic agent comprises a pharmaceutical composition described herein.
Therapeutically effective amount: As used herein, the term "therapeutically effective amount"
means an amount of a substance (e.g., a therapeutic agent, composition, and/or formulation) that elicits a desired biological response when administered as part of a therapeutic regimen. In some embodiments, a therapeutically effective amount of a substance is an amount that is sufficient, when administered to a subject suffering from or susceptible to a disease, disorder, and/or condition, to treat, diagnose, prevent, and/or delay the onset of the disease, disorder, and/or condition. As will be appreciated by those of ordinary skill in this art, an effective amount of a substance may vary depending on such factors as desired biological endpoint(s), substance to be delivered, target cell(s) or tissue(s), etc. For example, in some embodiments, an effective amount of compound in a formulation to treat a disease, disorder, and/or condition is an amount that alleviates, ameliorates, relieves, inhibits, prevents, delays onset of, reduces severity of and/or reduces incidence of one or more symptoms or features of the disease, disorder, and/or condition. In some embodiments, a therapeutically effective amount is administered in a single dose; in some embodiments, multiple unit doses are required to deliver a therapeutically effective amount.
BRIEF DESCRIPTION OF THE DRAWING
The following detailed description of the embodiments of the invention will be better understood when read in conjunction with the appended drawings. For the purpose of illustrating the invention, there .. are shown in the drawings embodiments, which are presently exemplified. It should be understood, however, that the invention is not limited to the precise arrangement and instrumentalities of the embodiments shown in the drawings.
Figure 1 shows a Genome Browser view showing key features of the region near the transcription start site of I32M. RNAseq (top level) shows mRNA expression from the I32M gene. A
previously described positive control (Cas9 targeting, GD-21547) is shown in green. Blue guides (GD-28228 and GD-28229) were used to target Sp-dCas9-KRAB and orange guides (GD-28171, GD-28172 and GD-28173) were used to target Sa-dCas9-MQ1 to the CpG Island in the I32M
promoter region (second level from the top). Guides were chosen based on hypomethylation of the promoter region (depicted in the CpG Methylation track, bottom level), where methylation runs from 0 to 1 (0 to 100%) and where higher methylation is depicted by red bars. Each bar represents a CpG site.
Figure 2 shows a graph of percent change in I3-2-microglobulin expression (mRNA) in HepG2 cells with treatment with a variety of expression repressors or controls. I32M
mRNA levels shown are relative to the untreated control (gray). Points show biological replicates.
Bars show the average and error bars indicate standard deviations. The guide ID number is shown on the x-axis.
Samples treated with dCas9 are shown in magenta; dCas9-KRAB in green; sa-dCas9-MQ1 in light blue; a combination of dCas9-KRAB and sa-dCas9-MQ1 in dark blue. A 132M-knockout positive control with Cas9 is shown in orange.
Figure 3 shows a graph of percent change in I3-2-microglobulin mRNA with treatment with a variety of expression repressors or controls after 72 hours (left), and a graph of flow cytometry data of 13-2-microglobulin protein levels with treatment with a variety of expression repressors or controls after 72 hours (right). (Left) Treatment with dCas9-KRAB (green) and Sa-dCas9-MQ1 (light blue) effectors, separately and together (dark blue) results in a decrease in I32M mRNA
relative to untreated cells (grey) and cells treated with dCas9 (magenta). (Right) Flow cytometry revealed populations with decreased I32M
immunofluorescence in cells treated with dCas9-KRAB and Sa-dCas9-MQ1 relative to untreated cells .. and cells treated with dCas9.
Figure 4 shows a graph of the fold change in I3-2-microglobulin mRNA with treatment with a variety of expression repressors or controls over a 22-day period. Over a 22-day period, non-transfected (blue line) and dCas9 transfected (red line) cells don't change I32M
expression, whereas dCas9-KRAB
(green line) transfected cells have I32M repressed in the initial days post-transfection, with maximum effect on day 2, but the repression is lost after day 5. SadCas9-MQ1 (purple line) transfected cells show significant repression (up to 85% by day 4) and the repression is retained and then slowly lost as seen by line remaining just under the dotted line in the figure. dCas9-KRAB + SadCas9-MQ1 combined transfection (orange line) did not give any added repression than what is already observed for SadCas9 alone (purple line).
Figure 5 shows a graph of the fold change in I3-2-microglobulin mRNA with treatment with a variety of expression repressors or controls over an 18-day period. Over an 18-day period untreated cells do not change I32M expression, whereas cells transfected with dCas9-MQ1, dCas9-DNMT3a/3L (m), dCas9-DNMT3a/3L (hs), dCas9-DNMT1 and dCas9-DNMT3B, show significant I32M
repression post-.. transfection.
Figure 6 shows a graph of the fold change in MYC expression in K-562 cells when treated with different expression repressors or expression repression systems that target the CTCF site located upstream of MYC gene. The data showed that, at least, cells treated with dCas9-DNMT3a/3L (h), dCas9-KRAB, FOG1-dCas9-FOG1, G9A-dCas9 + EZH2-dCas9, dCas9-MQ1 + EZH2-dCas9-KRAB, dCas9-LSD1+G9A-dCas9, dCas9-MQ1+dCas9-HDAC8, and dCas9-MQ1+G9A-dCas9-KRAB showed repression of MYC expression at 48 hour and/or 72-hour time point.
Figure 7 shows a graph of the fold change in I32M expression in K-562 cells when treated with different expression repressors or expression repression systems that target a region upstream of the I32M
promoter. The data showed that, at least, cells treated with dCas9-HDAC8, dCas9-LSD1, dCas9-MQ1, FOG1-dCas9- FOG1, G9A-dCas9-KRAB, dCas9-DNMT3a/3L (h), G9A-dCas9, EZH2-dCas9-KRAB, dCas9-KRAB, EZH2-dCas9-KRAB + dCas9-HDAC8, dCas9-LSD1 + EZH2-dCas9-KRAB, dCas9-LSD1 + dCas9-HDAC8, dCas9-KRAB + FOG1-dCas9- FOG1, dCas9-MQ1 + G9A-dCas9-KRAB, dCas9 + EZH2-dCas9, dCas9-MQ1 + EZH2-dCas9-KRAB, and dCas9-MQ1 + dCas9-HDAC8 showed repression of I32M expression at 48 hour, 72 hour and/or 144 hour time point.
Figure 8 shows a graph of the fold change in HSPA1B expression in K-562 cells when treated with different expression repressors or expression repression systems that target a region downstream of the HSPA1B promoter. The data showed that, at least, cells treated with dCas9-HDAC8, EZH2-dCas9, dCas9-MQ1, dCas9-DNMT1, dCas9-DNMT3a/3L (h), dCas9-DNMT3a/3L (m), G9A-dCas9-KRAB, FOG1-dCas9-FOG1, G9A-dCas9, dCas9-KRAB, G9A-dCas9 + dCas9-HDAC8, dCas9-LSD1 +
dCas9-KRAB, dCas9-LSD1+EZH2-dCas9, G9A-dCas9+EZH2-dCas9, dCas9-LSD1 + EZH2-dCas9-KRAB, EZH2-dCas9 +dCas9-HDAC8, dCas9-LSD1+dCas9-HDAC8, dCas9-MQ1 + EZH2-dCas9-KRAB, dCas9-MQ1+dCas9-HDAC8, G9A-dCas9-KRAB+EZH2-dCas9-KRAB, dCas9-LSD1+G9A-dCas9 and dCas9-MQ1+G9A-dCas9-KRAB showed repression of HSPA1B expression at 48 hour, 72 hour and/or 144 hour time point.
Figure 9 shows a graph of the fold change in GATA1 expression in K-562 cells when treated with different expression repressors or expression repression systems. The data showed that, at least, cells treated with dCas9-HDAC8, dCas9-LSD1+dCas9-HDAC8, dCas9-MQ1+dCas9-HDAC8, and dCas9+dCas9-HDAC8 showed repression of GATA1 expression at 48-hour, 72 hour and/or 144 hour 5 time point.
DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS
10 The present disclosure provides technologies for modulating, e.g., decreasing, expression of a target gene in cell, e.g., in a subject or patient, through the use of an expression repressor or an expression repression system. In some embodiments, an expression repressor comprises a DNA binding moiety and at least one repressor domain. In some embodiments, an expression repressor comprises two repressor domains. In some embodiments an expression repressor comprises two identical repressor domains. In 15 some embodiments an expression repressor comprises two non-identical repressor domains.
In some embodiments, an expression repression system comprises two or more expression repressors, each comprising a DNA-targeting moiety and at least one repressor domain. In some embodiments, the DNA-targeting moieties target two or more different DNA sequences (e.g., each expression repressor may target a different DNA sequence). Without wishing to be bound by theory, the use of an expression 20 repression system comprising expression repressors targeting two or more different DNA sequences may be advantageous over similar systems targeting a single DNA sequence because individual expression repressors will not compete with one another or compete less with one another for binding to their target DNA sequences, thereby achieving superior localization of the various functionalities of the repressor domains. In some embodiments, it can be advantageous to use two different DNA¨targeting moieties to 25 precisely target two different repressors to two different defined locations, e.g., a first expression repressor to a first location upstream of the transcription start site and a second expression repressor to a second location comprising the transcription start site. In some embodiments, the expression repressors comprise two or more different repressor domains (e.g., each expression repressor comprises a different repressor domain from each other expression repressor). Without wishing to be bound by theory, the use 30 of an expression repression system comprising expression repressors comprising two or more repressor domains may be advantageous over similar systems comprising a single repressor domain due to synergistic effects on expression of a target gene associated with the concerted action of the two or more repressor domains.
Expression Repressors Expression repressors of the present disclosure may comprise a DNA-targeting moiety and at least one repressor domain. In some embodiments, an expression repressor may comprise 1, 2, 3, 4, 5, 6, or more repressor domains. In some embodiments, an expression repressor targets two or more different DNA sequences (e.g., a Pt and 2nd, 3rd, 4th, 5th, 6th, 7th, 8th, V,--sth, 10th, 11th, 12th, and/or further DNA
sequence, and optionally no more than a 20th, 19th, 18th, 17th, 16th, 15th, 14th, 13th, 12th, llth, 10th, 9th, 8th, 6th, 5th, 4th, 3rd, or 2nd DNA sequence). In some embodiments, an expression repressor comprises a DNA-targeting moiety and a plurality of repressor domains (e.g., 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or more repressor domains (and optionally, less than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, or 2 repressor domains)) each of which may be the same or different from another of the more than one repressor domains.
In some embodiments, an expression repressor comprises a first repressor domain and a second repressor domain, wherein the first repressor domain is not identical to the second repressor domain. In some embodiments, an expression repressor comprises a first repressor domain and a second repressor domain, wherein the first repressor domain is identical to the second repressor domain. In some embodiments, the DNA-targeting moiety is situated between the first repressor domain and the second repressor domain.
An expression repressor may comprise a plurality of repressor domains, where each repressor domain comprises a different functionality than the other repressor domains.
For example, an expression repressor may comprise two repressor domains, where the first repressor domain comprises DNA
methylase functionality and the second repressor domain comprises a transcriptional repressor functionality. In some embodiments, an expression repressor comprises repressor domains whose functionalities are complementary to one another with regard to decreasing expression of a target gene, where the functionalities together enable inhibition of expression and, optionally, do not inhibit or negligibly inhibit expression when present individually. In some embodiments, an expression repressor comprises a plurality of repressor domains, wherein each repressor domain complements other repressor domains, each repressor domain decreases expression of a target gene.
In some embodiments, an expression repressor comprises a combination of repressor domains whose functionalities synergize with one another with regard to decreasing expression of a target gene.
Without wishing to be bound by theory, in some embodiments, epigenetic modifications to a genomic locus are cumulative, in that multiple transcription activating epigenetic markers (e.g., multiple different types of epigenetic markers and/or more extensive marking of a given type) individually together inhibit expression more effectively than individual modifications alone (e.g., producing a greater decrease in expression and/or a longer-lasting decrease in expression). In some embodiments, an expression repressor comprises a plurality of repressor domains, wherein each repressor domain synergizes with other repressor domains, e.g., each repressor domain decreases expression of a target gene. In some embodiments, an expression repressor (comprising a plurality of repressor domains which synergize with one another) is more effective at inhibiting expression of a target gene than an expression repressor comprising an individual repressor domain. In some embodiments, an expression repressor comprising said plurality of repressor domains is at least 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.55x, 1.6x, 1.65x, 1.7x, 1.75x, 1.8x, 1.85x, 1.9x, 1.95x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 30x, 40x, 50x, 60x, 70x, 80x, 90x, or 100x as effective at decreasing expression of a target gene than an expression repressor comprising an individual repressor domain.
In some embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain that are covalently linked, e.g., by a peptide bond. In some embodiments, the DNA-targeting moiety and the repressor domain are situated on the same polypeptide chain, e.g., connected by one or more peptide bonds and/or a linker. In some embodiments, the expression repressor is or comprises .. a fusion molecule, e.g., comprising the DNA-targeting moiety and the repressor domain linked by a peptide bond and/or a linker. In some embodiments, an expression repressor comprises a targeting moiety and a plurality of effector moieties, wherein the targeting moiety and the plurality of effector moieties are covalently linked, e.g., by peptide bonds (e.g., the targeting moiety and plurality of effector moieties are all connected by a series of covalent bonds, although each individual moiety may not share a covalent .. bond with every other effector moiety). In some embodiments, the expression repressor comprises a DNA-targeting moiety that is linked to the N-terminal of a repressor domain on the same polypeptide chain. In some embodiments, the expression repressor comprises a DNA-targeting moiety that is linked to the C-terminal of a repressor domain on the same polypeptide chain. In some embodiments, the expression repressor comprises a DNA-targeting moiety that is linked to the C-terminal of a first repressor domain and is linked to the N-terminal of a second repressor domain on the same polypeptide chain. In some embodiments, the expression repressor comprises a DNA-targeting moiety that is disposed N-terminal of a repressor domain on the same polypeptide chain. In some embodiments, the expression repressor comprises a DNA-targeting moiety that is disposed C-terminal of a repressor domain on the same polypeptide chain. In some embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain that are covalently linked by a non-peptide bond. In some embodiments, a DNA-targeting moiety is conjugated to a repressor domain by a non-peptide bond. In some embodiments, an expression repressor comprises a DNA-targeting moiety and a plurality of repressor domains, wherein the DNA-targeting moiety and the plurality of repressor domains are covalently linked, e.g., by peptide bonds (e.g., the DNA-targeting moiety and plurality of repressor domains are all connected by a series of covalent bonds, although each individual domain or moiety may not share a covalent bond with every other domain or moiety).
In other embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain that are not covalently linked, e.g., that are non-covalently associated with one another.
In some embodiments, an expression repressor comprises a DNA-targeting moiety that non-covalently binds to a repressor domain or vice versa. In some embodiments, an expression repressor comprises a DNA-targeting moiety and a plurality of repressor domains, wherein the DNA-targeting moiety and at least one repressor domain are not covalently linked, e.g., are non-covalently associated with one another, and wherein the DNA-targeting moiety and at least one other repressor domain are covalently linked, e.g., by a peptide bond.
In general, an expression repressor as described herein binds (e.g., via a DNA-targeting moiety) a genomic sequence element proximal to and/or operably linked to a target gene.
In some embodiments, binding of the expression repressor to the genomic sequence element modulates (e.g., decreases) expression of the target gene. For example, binding of an expression repressor comprising a repressor domain that recruits or inhibits recruitment of components of the transcription machinery to the genomic sequence element may modulate (e.g., decrease) expression of the target gene.
As a further example, binding of an expression repressor comprising a repressor domain with an enzymatic activity (e.g., an epigenetic modifying moiety) may modulate (e.g., decrease) expression of the target gene) through the localized enzymatic activity of the repressor domain. As a further example, both binding of an expression repressor to a genomic sequence element and the localized enzymatic activity of an expression repressor may contribute to the resulting modulation (e.g., decrease) in expression of the target gene.
In some embodiments, an expression repressor comprises a repressor domain wherein the repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, an expression repressor comprises a first repressor domain wherein the repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof and a second repressor domain wherein the repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain wherein the C-terminal end of the repressor domain, e.g., a repressor domain chosen from EZH1, EZH2, G9A, SUV39H1, FOG1, SETDB1, or SETDB2, and the N-terminal end of the DNA-5 targeting moiety are covalently linked. In some embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain wherein the N-terminal end of the repressor domain, e.g., a repressor domain chosen from LSD1, HDAC8, MQ1, DNMT1, DNMT3a/3L, FOG1, or KRAB, and the C-terminal end of the DNA-targeting moiety are covalently linked. In some embodiments, an expression repressor comprises a DNA-targeting moiety, a first repressor domain and second repressor domain, 10 wherein, the C-terminal end of the first repressor domain, e.g., a repressor domain chosen from EZH1, EZH2, G9A, SUV39H1, FOG1, SETDB1, or SETDB2, and the N-terminal end of the DNA-targeting moiety are covalently linked and the C-terminal end of the DNA-targeting moiety and the N-terminal end of the second repressor domain, e.g., a repressor domain chosen from LSD1, HDAC8, MQ1, DNMT1, DNMT3a/3L, FOG1, or KRAB are covalently linked.
15 In some embodiments, an expression repressor as disclosed herein is present in a composition, pharmaceutical composition, or mixture.
While several of the embodiments herein describe systems comprising a plurality of expression repressors, it is understood that the present application also provides the expression repressors individually, e.g., for use as a single agent, or for use in a combination with a generic second therapy that 20 need not be specified herein.
In some embodiments, an expression repressor described herein is part of an expression repression system, e.g., as described below.
Expression Repression Systems 25 Expression repression systems of the present disclosure may comprise two or more expression repressors. In some embodiments, an expression repression system comprises 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or more expression repressors (and optionally no more than 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, or 2). In some embodiments, an expression repression system targets two or more different DNA sequences (e.g., a 1st and 2nd, 3rd, 4th, 5th, 6th, 7th, 8th, V,--sth, 10th, 11th 12th, and/or further DNA sequence, and optionally 30 no more than a 20th, 19th, 18th, 17th, 16th, 15th, 14th, 13th, 12th, llth, 10th, 9th, 8th, 6th, 5th, 4th, 3rd, or 2nd DNA
sequence). In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each member of the plurality of expression repressors does not detectably bind, e.g., does not bind, to another member of the plurality of expression repressors. In some embodiments, an expression repression system comprises a first expression repressor and a second expression repressor, wherein the first expression repressor does not detectably bind, e.g., does not bind, to the second expression repressor.
In some embodiments, an expression repression system of the present disclosure comprises two or more expression repressors, wherein the expression repressors are present together in a composition, pharmaceutical composition, or mixture. In some embodiments, an expression repression system of the present disclosure comprises two or more expression repressors, wherein one or more expression repressors is not admixed with at least one other expression repressor. For example, an expression repression system may comprise a first expression repressor and a second expression repressor, wherein the presence of the first expression repressor in the nucleus of a cell does not overlap with the presence of the second expression repressor in the nucleus of the same cell, wherein the expression repression system achieves a decrease in expression of a target gene via the non-overlapping presences of the first and second expression repressors.
In some embodiments, the expression repressors of an expression repression system each comprise a different DNA-targeting moiety (e.g., the first, second, third, or further expression repressors each comprise different DNA-targeting moieties from one another). For example, an expression repression system may comprise a first expression repressor and a second expression repressor wherein the first expression repressor comprises a first DNA-targeting moiety (e.g., a Cas9 molecule, TAL
effector molecule, or Zn Finger domain), and the second expression repressor comprises a second DNA-targeting moiety (e.g., a Cas9 molecule, TAL effector molecule, or Zn Finger domain) different from the first DNA-targeting moiety. In some embodiments, different can mean comprising distinct types of DNA-targeting moiety, e.g., the first DNA-targeting moiety comprises a Cas9 molecule, and the second DNA-targeting moiety comprises a TAL effector molecule. In other embodiments, different can mean comprising distinct variants of the same type of DNA-targeting moiety, e.g., the first DNA-targeting moiety comprises a first Cas9 molecule (e.g., from a first species) and the second DNA-targeting moiety comprises a second Cas9 molecule (e.g., from a second species). In an embodiment, when an expression repression system comprises two or more DNA-targeting moieties of the same type, e.g., two or more Cas9 molecules, the DNA-targeting moieties specifically bind two or more different DNA sequences. For example, in an expression repression system comprising two or more Cas9 molecules, the two or more Cas9 molecules may be chosen or altered such that they only appreciably bind the gRNA corresponding to their target DNA sequence (e.g., and do not appreciably bind the gRNA
corresponding to the target of another Cas9 molecule). In a further example, in an expression repression system comprising two or more TAL effector molecules, the two or more TAL effector molecules may be chosen or altered such that they only appreciably bind to their target DNA sequence (e.g., and do not appreciably bind the target DNA
sequence of another TAL effector molecule).
In some embodiments, an expression repression system comprises three or more expression repressors and two or more expression repressors comprise the same DNA-targeting moiety. For example, an expression repression system may comprise three expression repressors, wherein the first and second expression repressors both comprise a first DNA-targeting moiety and the third expression repressor .. comprises a second different DNA-targeting moiety. For a further example, an expression repression system may comprise four expression repressors, wherein the first and second expression repressors both comprise a first DNA-targeting moiety and the third and fourth expression repressors comprises a second different DNA-targeting moiety. For a further example, an expression repression system may comprise five expression repressors, wherein the first and second expression repressors both comprise a first DNA-targeting moiety, the third and fourth expression repressors both comprise a second different DNA-targeting moiety, and the fifth expression repressor comprises a third different DNA-targeting moiety. As described above, different can mean comprising different types of DNA-targeting moieties or comprising distinct variants of the same type of DNA-targeting moiety.
In some embodiments, the expression repressors of an expression repression system each bind to a different DNA sequence (e.g., the first, second, third, or further expression repressors each bind DNA
sequences that are different from one another). For example, an expression repression system may comprise a first expression repressor and a second expression repressor wherein the first expression repressor binds to a first DNA sequence, and the second expression repressor binds to a second DNA
sequence. In some embodiments, different can mean that: there is at least one position that is not identical between the DNA sequence bound by one expression repressor and the DNA
sequence bound by another expression repressor, or that there is at least one position present in the DNA sequence bound by one expression repressor that is not present in the DNA sequence bound by another expression repressor. For example, a first expression repressor may bind to a first exemplary DNA
sequence 5'-ATGATTGGATTTA-3' (SEQ ID NO: 97), and a second expression repressor may bind to a second exemplary DNA sequence 5'- TGATTGGATTTAG-3' (SEQ ID NO: 98); in said example, the first and second exemplary DNA sequences are different. For a further example, a first expression repressor may bind to a first exemplary DNA sequence 5'-ATGATTgGATTTA-3' (SEQ ID NO: 99), and a second expression repressor may bind to a second exemplary DNA sequence 5'-ATGATTcGATTTA-3' (SEQ
ID NO: 100); in said example, the first and second exemplary DNA sequences are different. In some embodiments, the first DNA sequence may be situated on a first genomic DNA
strand and the second DNA sequence may be situated on a second genomic DNA strand. In some embodiments, the first DNA
sequence may be situated on the same genomic DNA strand as the second DNA
sequence.
In some embodiments, an expression repression system comprises three or more expression repressors and two or more expression repressors bind the same DNA sequence.
For example, an expression repression system may comprise three expression repressors, wherein the first and second expression repressors both bind a first DNA sequence, and the third expression repressor binds a second different DNA sequence. For a further example, an expression repression system may comprise four expression repressors, wherein the first and second expression repressors both bind a first DNA sequence and the third and fourth expression repressors both bind a second DNA
sequence. For a further example, an expression repression system may comprise five expression repressors, wherein the first and second expression repressors both bind a first DNA sequence, the third and fourth expression repressors both bind a second DNA sequence, and the fifth expression repressor binds a third DNA sequence. As described above, different can mean that there is at least one position that is not identical between the DNA sequence bound by one expression repressor and the DNA sequence bound by another expression repressor, or that there is at least one position present in the DNA sequence bound by one expression repressor that is not present in the DNA sequence bound by another expression repressor.
In some embodiments, an expression repression system comprises two or more (e.g., two) expression repressors and a plurality (e.g., two) of the expression repressors comprise DNA-targeting moieties that bind to different DNA sequences. In such embodiments, a first DNA-targeting moiety may bind to a first DNA sequence and a second DNA-targeting moiety may bind to a second DNA sequence, wherein the first and the second DNA sequences are different and do not overlap. In some such embodiments, the first DNA sequence is separated from the second DNA sequence by at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 base pairs (and optionally, no more than 500, 400, 300, 200, 100, 95, 90, 85, 80, 75, 70, 65, 60, 55, or 50 base pairs). In some such embodiments, the first DNA sequence is separated from the second DNA sequence by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 base pairs (and optionally, no base pairs, e.g., the first and second sequence are directly adjacent one another).
In some embodiments, the expression repressors of an expression repression system each comprise a different repressor domain (e.g., the first, second, third, or further expression repressors each comprise a different repressor domain from one another). For example, an expression repression system may comprise a first expression repressor and a second expression repressor wherein the first expression repressor comprises a first repressor domain (e.g., comprising a histone methyltransferase or functional fragment thereof), and the second expression repressor comprises a second repressor domain (e.g., comprising a DNA methyltransferase or functional fragment thereof) different from the first repressor domain. In some embodiments, different can mean comprising distinct types of repressor domain, e.g., the first repressor domain comprises a histone methyltransferase and the second repressor domain comprises a DNA methyltransferase, or the first repressor domain comprises a histone methyltransferase and the second repressor domain comprises a small molecule inhibitor of an enzyme. In other embodiments, different can mean comprising distinct variants of the same type of repressor domain, e.g., the first repressor domain comprises a first histone methyltransferase (e.g., having a first site specificity or amino acid sequence) and the second repressor domain comprises a second histone methyltransferase (e.g., .. having a second site specificity or amino acid sequence).
In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor domain, wherein the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, .. KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a .. functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, .. SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor .. domain, wherein the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, .. DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, 5 RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the third repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, 10 SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, an expression repression system comprises: (i) a first expression repressor comprising a first repressor domain and a third repressor domain, and (ii) a second expression repressor 15 comprising a second repressor domain and optionally a fourth repressor domain, wherein the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, 20 HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, 25 SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, 30 FOG1, SUZ12, or a functional variant or fragment thereof, the third repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B
(i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof and the fourth repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, .. DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, the first repressor domain comprises a histone methyltransferase activity (e.g., SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, or a functional variant or fragment of any thereof, e.g., a SET domain of any thereof) and the second repressor domain comprises a histone demethylase activity (e.g., KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, or a functional variant or fragment of any thereof). In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a histone deacetylase activity (e.g., HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof). In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a DNA
methyltransferase activity (e.g., MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, or a functional variant or fragment of any thereof). In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a DNA
demethylase activity. In some embodiments the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a transcription repressor activity (e.g., KRAB, MeCP2, HP1, RBBP4, .. REST, FOG1, SUZ12, or a functional variant or fragment of any thereof). In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a different histone methyltransferase activity. In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises the same histone methyltransferase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a histone deacetylase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a DNA methyltransferase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a DNA
demethylase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a transcription repressor activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a different histone demethylase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises the same histone demethylase activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a DNA
methyltransferase activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a DNA demethylase activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a transcription repressor activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a different histone deacetylase activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises the same histone deacetylase activity. In some embodiments, the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a DNA demethylase activity. In some embodiments, the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a transcription repressor activity. In some embodiments, the first repressor domain comprises a DNA
methyltransferase activity and the second repressor domain comprises a different DNA methyltransferase activity. In some embodiments, the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises the same DNA methyltransferase activity. In some embodiments, the first repressor domain comprises a DNA demethylase activity and the second repressor domain comprises a transcription repressor activity. In some embodiments, the first repressor domain comprises a DNA
demethylase activity and the second repressor domain comprises a different DNA
demethylase activity. In some embodiments, the first repressor domain comprises a DNA demethylase activity and the second .. repressor domain comprises the same DNA demethylase activity. In some embodiments, the first repressor domain comprises a transcription repressor activity and the second repressor domain comprises a different transcription repressor activity. In some embodiments, the first repressor domain comprises a transcription repressor activity and the second repressor domain comprises the same transcription repressor activity.
In some embodiments, an expression repression system comprises three or more expression repressors and two or more expression repressors comprise the same DNA-targeting moiety. For example, an expression repression system may comprise three expression repressors, wherein the first and second expression repressors both comprise a first repressor domain and the third expression repressor comprises a second different repressor domain. For a further example, an expression repression system may comprise four expression repressors, wherein the first and second expression repressors both comprise a first repressor domain and the third and fourth expression repressors comprises a second different repressor domain. For a further example, an expression repression system may comprise five expression repressors, wherein the first and second expression repressors both comprise a first repressor domain, the third and fourth expression repressors both comprise a second different repressor domain, and the fifth expression repressor comprises a third different repressor domain. As described above, different can mean comprising different types of repressor domain or comprising distinct variants of the same type of repressor domain.
In some embodiments, two repressor domains comprise a histone methyltransferase activity (e.g., SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, or a functional variant or fragment of any thereof, e.g., a SET domain of any thereof) and the other repressor domain comprises a histone demethylase activity (e.g., KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, or a functional variant or fragment of any thereof). In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a histone deacetylase activity (e.g., HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof). In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a DNA methyltransferase activity (e.g., MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, or a functional variant or fragment of any thereof). In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a DNA demethylase activity. In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a transcription repressor activity (e.g., KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment of any thereof). In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a different histone methyltransferase activity. In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises the same histone methyltransferase activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a histone deacetylase activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a DNA
methyltransferase activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a DNA demethylase activity.
In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a transcription repressor activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a different histone demethylase activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises the same histone demethylase activity. In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises a DNA
methyltransferase activity. In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises a DNA demethylase activity.
In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises a transcription repressor activity. In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises a different histone deacetylase activity. In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises the same histone deacetylase activity. In some embodiments, two repressor domains comprise a DNA methyltransferase activity and the other repressor domain comprises a DNA
demethylase activity. In some embodiments, two repressor domains comprise a DNA methyltransferase activity and the other repressor domain comprises a transcription repressor activity. In some embodiments, two repressor domains comprise a DNA methyltransferase activity and the other repressor domain comprises a different DNA methyltransferase activity. In some embodiments, two repressor domains comprise a DNA methyltransferase activity and the other repressor domain comprises the same DNA methyltransferase activity. In some embodiments, two repressor domains comprise a DNA
demethylase activity and the other repressor domain comprises a transcription repressor activity. In some embodiments, two repressor domains comprise a DNA demethylase activity and the other repressor domain comprises a different DNA demethylase activity. In some embodiments, two repressor domains comprise a DNA demethylase activity and the other repressor domain comprises the same DNA
demethylase activity. In some embodiments, two repressor domains comprise a transcription repressor activity and the other repressor domain comprises a different transcription repressor activity. In some embodiments, two repressor domains comprise a transcription repressor activity and the other repressor domain comprises the same transcription repressor activity.
In some embodiments, two or more (e.g., all) expression repressors of an expression repression system are not covalently associated with each other, e.g., each expression repressor is not covalently associated with any other expression repressor. In another embodiment, two or more expression repressors of an expression repression system are covalently associated with one another. In an 5 embodiment, an expression repression system comprises a first expression repressor and a second expression repressor disposed on the same polypeptide, e.g., as a fusion molecule, e.g., connected by a peptide bond and optionally a linker. In an embodiment, an expression repression system comprises a first expression repressor and a second expression repressor that are connected by a non-peptide bond, e.g., are conjugated to one another.
Linkers An expression repressor or an expression repression system as disclosed herein may comprise one or more linkers. A linker may connect a DNA-targeting moiety to a repressor domain, a repressor domain to another repressor domain, or a DNA-targeting moiety to another DNA-targeting moiety. A linker may be a chemical bond, e.g., one or more covalent bonds or non-covalent bonds. In some embodiments, a linker is covalent. In some embodiments, a linker is non-covalent. In some embodiments, a linker is a peptide linker. Such a linker may be between 2-30, 5-30, 10-30, 15-30, 20-30, 25-30, 2-25, 5-25, 10-25, 15-25, 20-25, 2-20, 5-20, 10-20, 15-20, 2-15, 5-15, 10-15, 2-10, 5-10, or 2-5 amino acids in length, or greater than or equal to 2, 5, 10, 15, 20, 25, or 30 amino acids in length (and optionally up to 50, 40, 30, 25, 20, 15, 10, or 5 amino acids in length). In some embodiments, a linker can be used to space a first domain or moiety from a second domain or moiety, e.g., a DNA-targeting moiety from a repressor domain. In some embodiments, for example, a linker can be positioned between a DNA-targeting moiety and a repressor domain, e.g., to provide molecular flexibility of secondary and tertiary structures. A
linker may comprise flexible, rigid, and/or cleavable linkers described herein. In some embodiments, a linker includes at least one glycine, alanine, and serine amino acids to provide for flexibility. In some embodiments, a linker is a hydrophobic linker, such as including a negatively charged sulfonate group, polyethylene glycol (PEG) group, or pyrophosphate diester group. In some embodiments, a linker is cleavable to selectively release a moiety (e.g., polypeptide) from a modulating agent, but sufficiently stable to prevent premature cleavage.
In some embodiments, one or more moieties and/or domains of an expression repressor described herein are linked with one or more linkers. In some embodiments, an expression repression may comprise a linker situated between the DNA-targeting moiety and the repressor domain. In some embodiments, an expression repressor may comprise a first linker situated between the DNA-targeting moiety and the first repressor domain, and a second linker situated between the DNA-targeting moiety and the second repressor domain. In some embodiments, the first and the second linker may be identical.
In some embodiments, the first and the second linker may be different.
As will be known by one of skill in the art, commonly used flexible linkers have sequences consisting primarily of stretches of Gly and Ser residues ("GS" linker).
Flexible linkers may be useful for joining domains/moieties that require a certain degree of movement or interaction and may include small, non-polar (e.g., Gly) or polar (e.g., Ser or Thr) amino acids. Incorporation of Ser or Thr can also maintain the stability of a linker in aqueous solutions by forming hydrogen bonds with water molecules, and therefore reduce unfavorable interactions between a linker and moieties/domains.
Rigid linkers are useful to keep a fixed distance between domains/moieties and to maintain their independent functions. Rigid linkers may also be useful when a spatial separation of domains is critical to preserve the stability or bioactivity of one or more components in the fusion.
Rigid linkers may have an alpha helix-structure or Pro-rich sequence, (XP)11, with X designating any amino acid, preferably Ala, Lys, or Glu.
Cleavable linkers may release free functional domains in vivo. In some embodiments, linkers may be cleaved under specific conditions, such as presence of reducing reagents or proteases. In vivo cleavable linkers may utilize reversible nature of a disulfide bond. One example includes a thrombin-sensitive sequence (e.g., PRS) between the two Cys residues. In vitro thrombin treatment of CPRSC
results in the cleavage of a thrombin-sensitive sequence, while a reversible disulfide linkage remains intact. Such linkers are known and described, e.g., in Chen et al. 2013.
Fusion Protein Linkers: Property, Design and Functionality. Adv Drug Deliv. Rev. 65(10): 1357-1369. /n vivo cleavage of linkers in fusions may also be carried out by proteases that are expressed in vivo under certain conditions, in specific cells or tissues, or constrained within certain cellular compartments. Specificity of many proteases offers slower cleavage of the linker in constrained compartments.
Examples of molecules suitable for use in linkers described herein include a negatively charged sulfonate group; lipids, such as a poly (--CH2--) hydrocarbon chains, such as polyethylene glycol (PEG) group, unsaturated variants thereof, hydroxylated variants thereof, amidated or otherwise N-containing variants thereof; noncarbon linkers; carbohydrate linkers; phosphodiester linkers, or other molecule capable of covalently linking two or more components of an expression repressor. Non-covalent linkers are also included, such as hydrophobic lipid globules to which the polypeptide is linked, for example through a hydrophobic region of a polypeptide or a hydrophobic extension of a polypeptide, such as a series of residues rich in leucine, isoleucine, valine, or perhaps also alanine, phenylalanine, or even tyrosine, methionine, glycine, or other hydrophobic residue. Components of an expression repressor may be linked using charge-based chemistry, such that a positively charged component of an expression repressor is linked to a negative charge of another component.
Nucleic acids In one aspect, the disclosure provides nucleic acid sequences encoding an expression repressor, an expression repression system, a DNA-targeting moiety and/or a repressor domain as described herein.
A skilled artisan is aware that the nucleic acid sequences of RNA are identical to the corresponding DNA
sequences, except that typically thymine (T) is replaced by uracil (U). It will be understood that when a nucleotide sequence is represented by a DNA sequence (e.g., comprising, A, T, G, C), this disclosure also provides the corresponding RNA sequence (e.g., comprising, A, U, G, C) in which "U" replaces "T."
Conventional notation is used herein to describe polynucleotide sequences: the left-hand end of a single-stranded polynucleotide sequence is the 5'-end; the left-hand direction of a double-stranded polynucleotide sequence is referred to as the 5'-direction.
It will be appreciated by those skilled in the art that due to the degeneracy of the genetic code, a multitude of nucleotide sequences encoding an expression repressor comprising DNA-targeting moiety and/or a repressor domain as described herein may be produced, some of which have similarity, e.g., 90%, 95%, 96%, 97%, 98%, or 99% identity to the nucleic acid sequences disclosed herein. For instance, codons AGA, AGG, CGA, CGC, CGG, and CGU all encode the amino acid arginine.
Thus, at every position in the nucleic acid molecules of the disclosure where an arginine is specified by a codon, the codon can be altered to any of the corresponding codons described above without altering the encoded polypeptide.
In some embodiments a nucleic acid sequence encoding an expression repressor comprising a DNA-targeting moiety and/or one or more repressor domains may be part or all of a codon-optimized coding region, optimized according to codon usage in mammals, e.g., humans. In some embodiments, a nucleic acid sequence encoding a DNA-targeting moiety and/or one or more repressor domains is codon optimized for increasing the protein expression and/or increasing the duration of protein expression. In some embodiments, a protein produced by the codon optimized nucleic acid sequence is at least 1%, at least 2%, at least 5%, at least 10%, at least 15%, at least 20%, at least 30%, at least 40%, or at least 50%
higher compared to levels of the protein when encoded by a nucleic acid sequence that is not codon optimized.
In one aspect, the disclosure is directed to a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, e.g., wherein the repressor domain is or comprises MQ1, e.g., bacterial MQ1, or a functional variant or fragment thereof. In some embodiments, MQ1 is Spiroplasma monobiae MQ1, e.g., MQ1 from strain ATCC 33825 and/or corresponding to Uniprot ID
P15840. In some embodiments, MQ1 repressor domain is encoded by a nucleotide sequence of SEQ ID
NO: 47. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ ID
NO: 47 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
AGCAAGGTGGAGAACAAGACCAAGAAGCTGCGGGTGTTCGAGGCCTTCGCCGGCATCGGCG
CCCAGCGGAAGGCCCTGGAGAAGGTGCGGAAGGACGAGTACGAGATCGTGGGCCTGGCCG
AGTGGTACGTGCCCGCCATCGTGATGTACCAGGCCATCCACAACAACTTCCACACCAAGCTG
GAGTACAAGAGCGTGAGCCGGGAGGAGATGATCGACTACCTGGAGAACAAGACCCTGAGCT
GGAACAGCAAGAACCCCGTGAGCAACGGCTACTGGAAGCGGAAGAAGGACGACGAGCTGA
AGATCATCTACAACGCCATCAAGCTGAGCGAGAAGGAGGGCAACATCTTCGACATCCGGGA
CCTGTACAAGCGGACCCTGAAGAACATCGACCTGCTGACCTACAGCTTCCCCTGCCAGGACC
TGAGCCAGCAGGGCATCCAGAAGGGCATGAAGCGGGGCAGCGGCACCCGGAGCGGCCTGCT
GTGGGAGATCGAGCGGGCCCTGGACAGCACCGAGAAGAACGACCTGCCCAAGTACCTGCTG
ATGGAGAACGTGGGCGCCCTGCTGCACAAGAAGAACGAGGAGGAGCTGAACCAGTGGAAG
CAGAAGCTGGAGAGCCTGGGCTACCAGAACAGCATCGAGGTGCTGAACGCCGCCGACTTCG
GCAGCAGCCAGGCCCGGCGGCGGGTGTTCATGATCAGCACCCTGAACGAGTTCGTGGAGCT
GCCCAAGGGCGACAAGAAGCCCAAGAGCATCAAGAAGGTGCTGAACAAGATCGTGAGCGA
GAAGGACATCCTGAACAACCTGCTGAAGTACAACCTGACCGAGTTCAAGAAAACCAAGAGC
AACATCAACAAGGCCAGCCTGATCGGCTACAGCAAGTTCAACAGCGAGGGCTACGTGTACG
ACCCCGAGTTCACCGGCCCCACCCTGACCGCCAGCGGCGCCAACAGCCGGATCAAGATCAA
GGACGGCAGCAACATCCGGAAGATGAACAGCGACGAGACCTTCCTGTACATCGGCTTCGAC
AGCCAGGACGGCAAGCGGGTGAACGAGATCGAGTTCCTGACCGAGAACCAGAAGATCTTCG
TGTGCGGCAACAGCATCAGCGTGGAGGTGCTGGAGGCCATCATCGACAAGATCGGCGGC
(SEQ ID NO: 47) In some embodiments, MQ1 comprises an amino acid sequence of SEQ ID NO: 90. In some embodiments, MQ1 comprises an amino acid sequence of SEQ ID NO: 57. In some embodiments, an effector domain described herein comprises SEQ ID NO: 90 or 57, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
MSKVENKTKKLRVFEAFAGIGAQRKALEKVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLE
YKSVSREEMIDYLENKTLSWNSKNPVSNGYWKRKKDDELKIIYNAIKLSEKEGNIFDIRDLYKRT
LKNIDLLTYSFPCQDLS QQGIQKGMKRGSGTRSGLLWEIERALD S TEKNDLPKYLLMENVGALL
HKKNEEELNQWKQKLESLGYQNSIEVLNAADFGS S QARRRVFMISTLNEFVELPKGDKKPKSIK
KVLNKIVSEKDILNNLLKYNLTEFKKTKSNINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSR
IKIKDGSNIRKMNSDETFLYIGFDSQDGKRVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGG (SEQ
ID NO: 90) SKVENKTKKLRVFEAFAGIGAQRKALEKVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEY
KSVSREEMIDYLENKTLSWNSKNPVSNGYWKRKKDDELKIIYNAIKLSEKEGNIFDIRDLYKRTL
KNIDLLTYSFPCQDLSQQGIQKGMKRGSGTRSGLLWEIERALDSTEKNDLPKYLLMENVGALLH
KKNEEELNQWKQKLESLGYQNSIEVLNAADFGSSQARRRVFMISTLNEFVELPKGDKKPKSIKK
VLNKIVSEKDILNNLLKYNLTEFKKTKSNINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRI
KIKDGSNIRKMNSDETFLYIGFDSQDGKRVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGG (SEQ
ID NO: 57) In some embodiments, MQ1 for use in a polypeptide described herein is a variant, e.g., comprising one or more mutations, relative to wildtype MQ1 (e.g., SEQ ID NO: 90 or SEQ ID NO:
57). In some embodiments, an MQ1 variant comprises one or more amino acid substitutions, deletions, or insertions relative to wildtype MQ1. In some embodiments, an MQ1 variant comprises a K297P substitution. In some embodiments, an MQ1 variant comprises a N299C substitution. In some embodiments, an MQ1 variant comprises a E301Y substitution. In some embodiments, an MQ1 variant comprises a Q147L
substitution (e.g., and has reduced DNA methyltransferase activity relative to wildtype MQ1). In some embodiments, an MQ1 variant comprises K297P, N299C, and E301Y substitutions (e.g., and has reduced DNA binding affinity relative to wildtype MQ1). In some embodiments, an MQ1 variant comprises Q147L, K297P, N299C, and E301Y substitutions (e.g., and has reduced DNA
methyltransferase activity and DNA binding affinity relative to wildtype MQ1). In some embodiments, the polypeptide comprises one or more linkers described herein, e.g., connecting a moiety/domain to another moiety/domain. In some embodiments, the polypeptide comprises a DNA-targeting moiety that is or comprises a CRISPR/Cas molecule, e.g., comprising a CRISPR/Cas protein, e.g., a dCas9 protein. In some embodiments, the polypeptide is a fusion protein comprising a repressor domain that is or comprises MQ1 and a DNA-targeting moiety that is or comprises a CRISPR/Cas molecule, e.g., comprising a CRISPR/Cas protein, e.g., a dCas9 protein. In some embodiments, the polypeptide comprises an additional moiety described herein. In some embodiments, the polypeptide decreases expression of a target gene (e.g., a target gene described herein). In some embodiments, the polypeptide may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene or transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising MQ1, e.g., bacterial MQ1, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide 5 .. comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises Krueppel-associated box (KRAB) e.g., as according to NP_056209.2 or the protein encoded by NM_015394.5 or a functional variant or fragment thereof. In some embodiments, KRAB is a synthetic KRAB construct. In some embodiments, KRAB comprises an amino acid sequence of SEQ ID NO: 61:
DAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVIL
RLEKGEEPWLVEREIHQETHPDSETAFEIKSSV (SEQ ID NO: 61) In some embodiments, the KRAB repressor domain is encoded by a nucleotide sequence of SEQ
ID NO: 51. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ
ID NO: 51 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
GACGCCAAGAGCCTGACCGCCTGGAGCCGGACCCTGGTGACCTTCAAGGACGTGTTCGTGG
ACTTCACCCGGGAGGAGTGGAAGCTGCTGGACACCGCCCAGCAGATCCTGTACCGGAACGT
GATGCTGGAGAACTACAAGAACCTGGTGAGCCTGGGCTACCAGCTGACCAAGCCCGACGTG
ATCCTGCGGCTGGAGAAGGGCGAGGAGCCCTGGCTGGTGGAGCGGGAGATCCACCAGGAGA
CCCACCCCGACAGCGAGACCGCCTTCGAGATCAAGAGCAGCGTG (SEQ ID NO: 51) In some embodiments, KRAB for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the KRAB sequence of SEQ ID
NO: 61. In some embodiments, an KRAB variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 61.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises KRAB and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the KRAB sequence of SEQ ID NO: 61, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, wherein the repressor domain is or comprises DNMT1, e.g., human DNMT1, or a functional variant or fragment thereof In some embodiments, DNMT1 is human DNMT1, e.g., corresponding to Gene ID 1786, e.g., corresponding to UniPort ID P26358.2. In some embodiments, DNMT1 comprises an amino acid sequence of SEQ ID NO: 58. In some embodiments, a repressor domain described herein comprises a sequence according to SEQ ID NO: 58 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto:
VDLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILL
KLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYY
RPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGE
KLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISY
NGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMA
RKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLY
GRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVG
NAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD (SEQ ID NO: 58) In some embodiments, DNMT1 is encoded by a nucleotide sequence of SEQ ID NO:
48. In some embodiments, a nucleic acid described herein comprises a sequence of SEQ ID
NO: 48 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto:
GTGGATCTGAGGACACTCGACGTGTTTAGCGGATGCGGCGGACTCTCCGAAGGCTTCCACCA
AGCCGGAATTTCCGACACACTCTGGGCCATTGAGATGTGGGACCCCGCCGCTCAAGCCTTCA
GACTGAATAATCCCGGCTCCACCGTGTTCACCGAGGACTGCAACATTCTGCTGAAGCTGGTG
ATGGCTGGCGAAACCACCAACTCTAGAGGCCAGAGGCTGCCCCAGAAGGGAGATGTGGAAA
TGCTCTGTGGAGGCCCTCCTTGCCAAGGCTTCTCCGGCATGAACAGGTTCAACTCTAGAACA
TACAGCAAGTTCAAGAACTCTCTGGTCGTGAGCTTTCTGAGCTACTGCGACTACTATAGACC
TAGGTTCTTTCTGCTGGAGAACGTGAGAAATTTCGTGTCCTTCAAGAGGAGCATGGTGCTGA
AGCTGACACTGAGGTGTCTGGTGAGGATGGGCTACCAGTGCACATTCGGAGTGCTGCAAGCT
GGCCAGTACGGCGTGGCCCAGACCAGAAGGAGGGCCATCATTCTGGCTGCTGCCCCCGGCG
AGAAACTCCCTCTGTTCCCCGAGCCCCTCCACGTGTTCGCCCCTAGAGCTTGCCAGCTGAGC
GTGGTGGTCGACGATAAGAAGTTCGTGAGCAACATCACAAGGCTGTCCAGCGGACCCTTCA
GAACCATTACCGTGAGGGATACCATGTCCGACCTCCCCGAGGTGAGGAATGGCGCCAGCGC
TCTGGAGATTTCCTACAACGGCGAACCTCAGAGCTGGTTCCAAAGGCAGCTGAGAGGCGCTC
AGTATCAGCCCATTCTGAGGGACCACATCTGCAAAGATATGAGCGCTCTGGTGGCCGCTAGA
ATGAGACATATTCCTCTGGCCCCCGGCAGCGACTGGAGAGATCTGCCCAATATTGAGGTGAG
ACTCAGCGACGGAACAATGGCTAGAAAACTGAGGTACACCCATCATGATAGAAAGAACGGA
AGGAGCAGCAGCGGCGCTCTGAGAGGAGTGTGTAGCTGCGTGGAAGCTGGCAAGGCTTGCG
ATCCCGCCGCTAGGCAGTTCAATACCCTCATCCCTTGGTGTCTGCCTCACACCGGCAACAGA
CACAATCATTGGGCTGGACTGTATGGAAGGCTCGAATGGGACGGCTTTTTCAGCACCACCGT
GACCAATCCCGAACCTATGGGCAAGCAAGGAAGGGTGCTCCACCCCGAGCAGCATAGAGTC
GTGTCCGTGAGAGAATGCGCTAGAAGCCAAGGCTTCCCCGACACCTATAGACTGTTCGGCAA
CATTCTGGATAAGCACAGACAAGTGGGAAATGCTGTCCCTCCTCCTCTGGCCAAGGCTATCG
GACTGGAGATCAAGCTGTGTATGCTCGCCAAAGCTAGGGAGAGCGCTTCCGCCAAGATTAA
GGAGGAGGAGGCCGCCAAGGAC (SEQ ID NO: 48) In some embodiments, DNMT1 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to a DNMT sequence of SEQ ID NO:
58. In some embodiments, the effector domain comprises one or more amino acid substitutions, deletions, or insertions relative to wild type DNMT1. In some embodiments, the polypeptide is a fusion protein comprising a repressor domain that is or comprises DNMT1 and a targeting moiety. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising DNMT1, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, wherein the repressor domain is or comprises DNMT3a/3L complex, or a functional variant or fragment thereof. In some embodiments, the DNMT3a/3L complex is a fusion construct. In some embodiments the DNMT3a/3L complex comprises DNMT3A, e.g., human DNMT3A, e.g., as according to NP_072046.2 or the protein encoded by NM_022552.4). In some embodiments the DNMT3a/3L
complex comprises mouse DNMT3A, e.g., as according to NP_031898 or the protein encoded by NM_007872. In some embodiments the DNMT3a/3L complex comprises human DNMT3L (e.g., as according to NP_787063.1 or the protein encoded by NM_175867.3). In some embodiments the DNMT3a/3L
complex comprises mouse DNMT3L (e.g., as according to NP_001075164 or the protein encoded by NM_001081695). In some embodiments, DNMT3a/3L comprises an amino acid sequence of SEQ ID NO:59 or 60. In some embodiments, a repressor domain described herein comprises SEQ ID NO: 59 or SEQ ID NO: 60, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
NHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRH
QGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDA
RPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRP
LASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFG
FPVHYTDV SNMSRLARQRLLGRSWS VPVIRHLFAPLKEYFACVS SGNSNANSRGPSFS SGLVPLS
LRGSHMNPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKD
VEEWGPFDLVYGATPPLGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDL
DVASRFLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALV SEEELSLLAQNKQS SKLAAKW
PTKLVKNCFLPLREYFKYFSTELTSSL (SEQ ID NO: 59) NHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRH
QGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDA
RPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRP
LASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFG
FPVHYTDV SNMSRLARQRLLGRSWS VPVIRHLFAPLKEYFACVS SGNSNANSRGPSFS SGLVPLS
LRGSHMGPMEIYKTVSAWKRQPVRVLSLFRNIDKVLKSLGFLESGSGSGGGTLKYVEDVTNVVR
RDVEKWGPFDLVYGSTQPLGS SCDRCPGWYMFQFHRILQYALPRQES QRPFFWIFMDNLLLTED
DQETTTRFLQTEAVTLQDVRGRDYQNAMRVWSNIPGLKSKHAPLTPKEEEYLQAQVRSRSKLD
APKVDLLVKNCLLPLREYFKYFSQNSLPL (SEQ ID NO: 60) In some embodiments, DNMT3a/3L is encoded by a nucleotide sequence of SEQ ID
NO: 49 or SEQ ID NO: 50. In some embodiments, a nucleic acid described herein comprises a sequence of SEQ ID
NO: 49 or 50 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
AACCACGACCAGGAGTTCGACCCCCCCAAGGTGTACCCCCCCGTGCCCGCCGAGAAGCGGA
AGCCCATCCGGGTGCTGAGCCTGTTCGACGGCATCGCCACCGGCCTGCTGGTGCTGAAGGAC
CTGGGCATCCAGGTGGACCGGTACATCGCCAGCGAGGTGTGCGAGGACAGCATCACCGTGG
GCATGGTGCGGCACCAGGGCAAGATCATGTACGTGGGCGACGTGCGGAGCGTGACCCAGAA
GCACATCCAGGAGTGGGGCCCCTTCGACCTGGTGATCGGCGGCAGCCCCTGCAACGACCTG
AGCATCGTGAACCCCGCCCGGAAGGGCCTGTACGAGGGCACCGGCCGGCTGTTCTTCGAGTT
CTACCGGCTGCTGCACGACGCCCGGCCCAAGGAGGGCGACGACCGGCCCTTCTTCTGGCTGT
TCGAGAACGTGGTGGCCATGGGCGTGAGCGACAAGCGGGACATCAGCCGGTTCCTGGAGAG
CAACCCCGTGATGATCGACGCCAAGGAGGTGAGCGCCGCCCACCGGGCCCGGTACTTCTGG
GGCAACCTGCCCGGCATGAACCGGCCCCTGGCCAGCACCGTGAACGACAAGCTGGAGCTGC
AGGAGTGCCTGGAGCACGGCCGGATCGCCAAGTTCAGCAAGGTGCGGACCATCACCACCCG
GAGCAACAGCATCAAGCAGGGCAAGGACCAGCACTTCCCCGTGTTCATGAACGAGAAGGAG
GACATCCTGTGGTGCACCGAGATGGAGCGGGTGTTCGGCTTCCCCGTGCACTACACCGACGT
GAGCAACATGAGCCGGCTGGCCCGGCAGCGGCTGCTGGGCCGGAGCTGGAGCGTGCCCGTG
ATCCGGCACCTGTTCGCCCCCCTGAAGGAGTACTTCGCCTGCGTGAGCAGCGGCAACAGCAA
CGCCAACAGCCGGGGCCCCAGCTTCAGCAGCGGCCTGGTGCCCCTGAGCCTGCGGGGCAGC
CACATGAATCCTCTGGAGATGTTCGAGACAGTGCCCGTGTGGAGAAGGCAACCCGTGAGGG
TGCTGAGCCTCTTCGAGGACATTAAGAAGGAGCTGACCTCTCTGGGCTTTCTGGAATCCGGC
AGCGACCCCGGCCAGCTGAAACACGTGGTGGACGTGACCGACACAGTGAGGAAGGACGTGG
AAGAGTGGGGCCCCTTTGACCTCGTGTATGGAGCCACACCTCCTCTCGGCCACACATGCGAT
AGGCCTCCCAGCTGGTATCTCTTCCAGTTCCACAGACTGCTCCAGTACGCCAGACCTAAGCC
CGGCAGCCCCAGACCCTTCTTCTGGATGTTCGTGGACAATCTGGTGCTGAACAAGGAGGATC
TGGATGTGGCCAGCAGATTTCTGGAGATGGAACCCGTGACAATCCCCGACGTGCATGGCGG
CTCTCTGCAGAACGCCGTGAGAGTGTGGTCCAACATCCCCGCCATTAGAAGCAGACACTGGG
CTCTGGTGAGCGAGGAGGAACTGTCTCTGCTGGCCCAGAATAAGCAGTCCTCCAAGCTGGCC
GCCAAGTGGCCCACCAAGCTGGTGAAGAACTGCTTTCTGCCTCTGAGGGAGTATTTCAAGTA
TTTCAGCACCGAACTGACCAGCAGCCTG (SEQ ID NO: 49) AACCACGACCAGGAGTTCGACCCCCCCAAGGTGTACCCCCCCGTGCCCGCCGAGAAGCGGA
AGCCCATCCGGGTGCTGAGCCTGTTCGACGGCATCGCCACCGGCCTGCTGGTGCTGAAGGAC
CTGGGCATCCAGGTGGACCGGTACATCGCCAGCGAGGTGTGCGAGGACAGCATCACCGTGG
GCATGGTGCGGCACCAGGGCAAGATCATGTACGTGGGCGACGTGCGGAGCGTGACCCAGAA
GCACATCCAGGAGTGGGGCCCCTTCGACCTGGTGATCGGCGGCAGCCCCTGCAACGACCTG
AGCATCGTGAACCCCGCCCGGAAGGGCCTGTACGAGGGCACCGGCCGGCTGTTCTTCGAGTT
CTACCGGCTGCTGCACGACGCCCGGCCCAAGGAGGGCGACGACCGGCCCTTCTTCTGGCTGT
TCGAGAACGTGGTGGCCATGGGCGTGAGCGACAAGCGGGACATCAGCCGGTTCCTGGAGAG
CAACCCCGTGATGATCGACGCCAAGGAGGTGAGCGCCGCCCACCGGGCCCGGTACTTCTGG
GGCAACCTGCCCGGCATGAACCGGCCCCTGGCCAGCACCGTGAACGACAAGCTGGAGCTGC
AGGAGTGCCTGGAGCACGGCCGGATCGCCAAGTTCAGCAAGGTGCGGACCATCACCACCCG
GAGCAACAGCATCAAGCAGGGCAAGGACCAGCACTTCCCCGTGTTCATGAACGAGAAGGAG
GAGCAACATGAGCCGGCTGGCCCGGCAGCGGCTGCTGGGCCGGAGCTGGAGCGTGCCCGTG
ATCCGGCACCTGTTCGCCCCCCTGAAGGAGTACTTCGCCTGCGTGAGCAGCGGCAACAGCAA
CGCCAACAGCCGGGGCCCCAGCTTCAGCAGCGGCCTGGTGCCCCTGAGCCTGCGGGGCAGC
CACATGGGCCCCATGGAGATCTACAAGACCGTGAGCGCCTGGAAGCGGCAGCCCGTGCGGG
AGCGGCAGCGGCGGCGGCACCCTGAAGTACGTGGAGGACGTGACCAACGTGGTGCGGCGGG
ACGTGGAGAAGTGGGGCCCCTTCGACCTGGTGTACGGCAGCACCCAGCCCCTGGGCAGCAG
CTGCGACCGGTGCCCCGGCTGGTACATGTTCCAGTTCCACCGGATCCTGCAGTACGCCCTGC
CCCGGCAGGAGAGCCAGCGGCCCTTCTTCTGGATCTTCATGGACAACCTGCTGCTGACCGAG
GGGGCCGGGACTACCAGAACGCCATGCGGGTGTGGAGCAACATCCCCGGCCTGAAATCCAA
GCACGCCCCCCTGACCCCCAAGGAGGAGGAGTACCTGCAGGCCCAGGTGCGGAGCCGGAGC
AAGCTGGACGCCCCCAAGGTGGACCTGCTGGTGAAGAACTGCCTGCTGCCCCTGCGGGAGT
ACTTCAAGTACTTCAGCCAGAACAGCCTGCCCCTG (SEQ ID NO: 50) In some embodiments, DNMT3a/3L for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the DNMT3a/3L of SEQ ID NO:
59 or SEQ ID NO: 60. In some embodiments, an DNMT3a/3L variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 59 or SEQ ID
NO: 60. In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises DNMT3a/3L and a DNA-targeting moiety. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising DNMT3a/3L, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, wherein the repressor domain is or comprises DNMT3b, e.g., human DNMT3b, or a functional variant or fragment thereof. In some embodiments the DNMT3b is human DNMT3b e.g., as according to NP_008823.1 or A0X21819.1, or the protein encoded by NM_006892.4 or KX447429.) In some embodiments, DNMT3b comprises an amino acid sequence of SEQ ID NO: 85. In some embodiments, a repressor domain described herein comprises SEQ ID NO: 85, or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
DNMT3b MKGDTRHLNGEEDAGGRED SILVNGAC SD QS SD SPPILEAIRTPEIRGRRS S SRLS KREVS SLLS YT
QDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRN
HVDESPVEFPATRS LRRRATAS AGTPWPSPPS S YLTIDLTDDTEDTHGTPQS S S TPYARLAQD S QQ
GGMESPQVEADSGDGDSSEYQVSADKLVALGLFSQHFNLATFNKLVSYRKAMYHALEKARVR
AGKTFPSSPGDSLEDQLKPMLEWAHGGFKPTGIEGLKPNNTQPENKTRRRTADDSATSDYCPAP
KRLKTNCYNNGKDRGDEDQSREQMASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCR
DRFLELFYMYDDDGYQSYCTVCCEGRELLLCSNTSCCRCFCVECLEVLVGTGTAAEAKLQEPWS
CYMCLPQRCHGVLRRRKDWNVRLQAFFTSDTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGY
LVLKELGIKVGKYVASEVCEESIAVGTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCND
LSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRFLECN
PVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEYNRIAKLKKVQTITTKSNSIK
QGKNQLFPVVMNGKEDVLWCTELERIFGFPVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAP
LKDYFACE (SEQ ID NO: 85) In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises G9A e.g., as according to NP_001350618.1 or the protein encoded by NM_001363689.1 or a functional variant or fragment thereof. In some embodiments, G9A comprises an amino acid sequence of SEQ ID NO: 62:
GNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCV
DDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQL
YRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYG
NISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGS
EKCKHSAEAIALEQSRLARLD (SEQ ID NO: 62) In some embodiments, the G9A repressor domain is encoded by a nucleotide sequence of SEQ ID
NO: 52. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ ID
NO: 52 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
GGAAATAGGGCTATCAGAACCGAGAAGATCATCTGTAGGGACGTGGCTAGAGGCTACGAGA
ACGTGCCCATTCCTTGCGTGAATGGCGTGGATGGCGAACCTTGCCCCGAGGACTACAAATAC
ATCTCCGAGAACTGCGAAACCAGCACAATGAACATCGACAGAAACATCACCCACCTCCAGC
ACTGCACATGTGTGGATGACTGCTCCTCCAGCAACTGTCTGTGCGGCCAGCTCTCCATCAGA
TGCTGGTACGACAAGGACGGCAGACTGCTGCAAGAGTTCAACAAGATCGAACCCCCTCTCA
TCTTCGAGTGTAACCAAGCTTGCAGCTGCTGGAGAAACTGCAAGAATAGAGTGGTCCAGAG
CGGCATCAAGGTGAGACTGCAACTGTACAGAACCGCCAAGATGGGATGGGGAGTGAGGGCT
CTGCAAACCATTCCCCAAGGCACCTTCATCTGCGAATACGTGGGCGAACTGATCTCCGACGC
CGAAGCTGACGTGAGAGAGGACGACAGCTATCTCTTCGATCTGGACAATAAGGACGGCGAG
GTGTACTGCATCGACGCTAGATATTACGGCAACATCTCTAGATTCATCAACCACCTCTGCGA
TCCCAACATCATTCCCGTGAGGGTGTTCATGCTGCACCAAGATCTGAGGTTCCCTAGAATCG
CCTTCTTCAGCTCTAGAGACATCAGAACCGGCGAGGAGCTGGGCTTCGATTACGGCGATAGA
TTCTGGGACATCAAGTCCAAGTACTTCACATGCCAGTGCGGCAGCGAGAAGTGTAAGCACA
GCGCTGAGGCCATTGCTCTGGAGCAGTCTAGACTGGCCAGACTGGAT (SEQ ID NO: 52) In some embodiments, G9A for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the G9A
sequence of SEQ ID NO: 62. In some embodiments, an G9A variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 62.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises G9A and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the G9A sequence of SEQ ID NO: 62, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises HDAC8, e.g., as according to NP_001159890 or the protein encoded by NM_001166418 or a functional variant or fragment thereof. In some embodiments, HDAC8 comprises an amino acid sequence of SEQ ID NO: 63:
EEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEE
MATFHTDAYLQHLQKVS QEGDDDHPD SIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLID GM
CKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTS
KVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNP
KAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTG
VILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV (SEQ ID NO:
63) In some embodiments, the HDAC8 repressor domain is encoded by a nucleotide sequence of SEQ ID NO: 53. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ ID NO: 53 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
GAGGAGCCCGAGGAGCCCGCCGATAGCGGACAATCTCTGGTGCCCGTCTACATCTACAGCC
CCGAATATGTGAGCATGTGTGATTCCCTCGCCAAGATCCCTAAGAGAGCCAGCATGGTGCAT
TCTCTGATCGAGGCCTACGCTCTGCATAAGCAAATGAGGATCGTGAAGCCCAAGGTCGCCAG
CATGGAAGAGATGGCCACCTTTCACACCGATGCCTACCTCCAACATCTCCAGAAGGTGTCCC
AAGAGGGCGACGACGACCACCCCGACTCCATTGAGTACGGACTGGGCTATGATTGCCCCGC
CACCGAGGGCATCTTTGACTATGCCGCCGCTATCGGCGGAGCTACCATCACAGCCGCCCAGT
GTCTGATTGATGGCATGTGCAAGGTCGCCATCAACTGGTCCGGAGGCTGGCATCATGCCAAG
AAGGATGAGGCCTCCGGCTTCTGTTATCTGAATGACGCCGTGCTGGGCATTCTGAGACTGAG
GAGGAAATTCGAGAGGATTCTGTACGTGGATCTGGATCTGCATCACGGAGATGGAGTCGAA
GATGCCTTCAGCTTCACCAGCAAGGTGATGACAGTCTCTCTGCACAAGTTCTCCCCCGGCTTC
TTTCCCGGAACCGGCGACGTGTCCGACGTGGGACTGGGCAAGGGAAGGTACTACAGCGTGA
ACGTGCCCATTCAAGACGGCATCCAAGACGAGAAGTACTACCAGATCTGCGAGTCCGTGCTC
AAGGAGGTCTACCAAGCCTTCAATCCTAAGGCTGTCGTGCTCCAACTGGGAGCTGATACCAT
TGCTGGCGATCCCATGTGCAGCTTCAATATGACACCCGTCGGAATCGGCAAGTGCCTCAAGT
ACATCCTCCAGTGGCAGCTCGCCACCCTCATTCTCGGAGGAGGCGGATACAATCTGGCTAAT
ACCGCCAGATGCTGGACCTATCTGACCGGCGTGATTCTGGGCAAAACACTGAGCAGCGAAA
TCCCCGACCACGAGTTTTTCACCGCTTACGGCCCCGACTACGTGCTGGAGATCACCCCCAGC
TGCAGACCCGATAGAAACGAACCCCATAGAATCCAGCAAATTCTGAACTATATCAAGGGCA
ACCTCAAGCACGTCGTG (SEQ ID NO: 53) In some embodiments, HDAC8 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the HDAC8 sequence of SEQ ID
NO: 63. In some embodiments, an HDAC8 variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 63.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises HDAC8 and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the HDAC8 sequence of SEQ ID NO: 63, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises LSD1 e.g., as according to NP_055828.2 or the protein encoded by NM_015013.4 or a functional variant or fragment thereof. In some embodiments, KRAB
comprises an amino acid sequence of SEQ ID NO: 64:
LSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKK
EPPRASPPGGLAEPPGSAGPQAGPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLA
NLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEA
ACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERH
GLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGN
YVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRL
LEATSYLSHQLDFNVLNNKHVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKM
VNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEA
NPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVA
LAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPP
LVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSS
GNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYT
LPRQATPGVPAQQSPSM (SEQ ID NO: 64) 10 In some embodiments, the LSD1 repressor domain is encoded by a nucleotide sequence of SEQ
ID NO: 54. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ
ID NO: 54 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
AGCTGGCCCCGGAACAGCTGGCGGAAGCGAAAACGGAAGCGAAGTGGCTGCCCAGCCCGCC
GGACTGTCCGGACCCGCCGAGGTGGGACCCGGCGCTGTCGGCGAAAGGACCCCCAGAAAAA
AGGAGCCTCCTAGAGCCAGCCCTCCCGGCGGACTCGCCGAACCTCCCGGCAGCGCTGGACCT
CAAGCCGGACCTACAGTGGTGCCCGGCAGCGCTACACCTATGGAGACCGGCATCGCTGAGA
ATGAGTCTCTGGCTAACCTCAGCGAGGACGAGTACTACTCCGAGGAGGAAAGGAATGCCAA
GGCCGAGAAGGAGAAGAAGCTGCCCCCTCCTCCCCCTCAAGCCCCCCCCGAGGAGGAGAAC
GAGTCCGAGCCCGAGGAACCCAGCGGAGTGGAAGGAGCCGCCTTTCAGTCCAGACTGCCCC
ACGACAGAATGACATCCCAAGAGGCCGCTTGCTTTCCCGACATTATTTCCGGCCCTCAGCAG
GATCCAGCTGACCTTCGAGGCTACCCTCCAACAGCTGGAGGCCCCCTACAATAGCGATACCG
TGCTGGTGCACAGAGTGCACAGCTATCTGGAGAGGCACGGCCTCATTAACTTCGGCATTTAC
AAGCGGATCAAGCCCCTGCCCACCAAGAAAACAGGCAAGGTGATCATCATCGGCAGCGGCG
TGAGCGGCCTGGCCGCCGCCCGGCAGCTGCAGAGCTTCGGCATGGACGTGACCCTGCTGGA
CTGGGCGCCATGGTGGTGACCGGCCTGGGCGGCAACCCCATGGCCGTGGTGAGCAAGCAGG
TGAACATGGAGCTGGCCAAGATCAAGCAGAAGTGCCCCCTGTACGAGGCCAACGGCCAGGC
CGTGCCCAAGGAGAAGGACGAGATGGTGGAGCAGGAGTTCAACCGGCTGCTGGAGGCCACC
AGCTACCTGAGCCACCAGCTGGACTTCAACGTGCTGAACAACAAGCACGTGAGCCTGGGCC
AGGCCCTGGAGGTGGTGATCCAGCTGCAGGAGAAGCACGTGAAGGACGAGCAGATCGAGC
ACTGGAAGAAGATCGTGAAAACACAGGAGGAGCTGAAGGAGCTGCTGAACAAGATGGTGA
ACCTGAAGGAGAAGATCAAGGAGCTGCACCAGCAGTACAAGGAGGCCAGCGAGGTGAAGC
CCCCCCGGGACATCACCGCCGAGTTCCTGGTCAAAAGCAAGCACCGGGACCTGACCGCCCT
GTGCAAGGAGTACGACGAGCTGGCCGAGACCCAGGGCAAGCTGGAGGAGAAGCTGCAGGA
GCTGGAGGCCAACCCCCCCAGCGACGTGTACCTGAGCAGCCGGGACCGGCAGATCCTGGAC
TGGCACTTCGCCAACCTGGAGTTCGCCAACGCCACCCCCCTGAGCACCCTGAGCCTGAAGCA
CTGGGACCAGGACGACGACTTCGAGTTCACCGGCAGCCACCTGACCGTGCGGAACGGCTAC
AGCTGCGTGCCCGTGGCCCTGGCCGAGGGCCTGGACATCAAGCTGAACACCGCCGTGCGGC
AGGTGCGGTACACCGCCAGCGGCTGCGAGGTGATCGCCGTGAACACCCGGAGCACCAGCCA
GACCTTCATCTACAAGTGCGACGCCGTGCTGTGCACCCTGCCCCTGGGCGTGCTGAAGCAGC
AGCCCCCCGCCGTGCAGTTCGTGCCCCCCCTGCCCGAGTGGAAAACAAGCGCCGTGCAGCG
GATGGGCTTCGGCAACCTGAACAAGGTGGTGCTGTGCTTCGACCGGGTGTTCTGGGACCCCA
GCGTGAACCTGTTCGGCCACGTGGGCAGCACCACCGCCAGCCGGGGCGAGCTGTTCCTGTTC
TGGAACCTGTACAAGGCCCCCATCCTGCTGGCCCTGGTGGCCGGCGAGGCCGCCGGCATCAT
GGAGAACATCAGCGACGACGTGATCGTGGGCCGGTGCCTGGCCATCCTGAAGGGCATCTTC
GGCAGCAGCGCCGTGCCCCAGCCCAAGGAGACCGTGGTGAGCCGGTGGCGGGCCGACCCCT
GGGCCCGGGGCAGCTACAGCTACGTGGCCGCCGGCAGCAGCGGCAACGACTACGACCTGAT
GGCCCAGCCCATCACCCCCGGCCCCAGCATCCCCGGCGCCCCCCAGCCCATCCCCCGGCTGT
TCTTCGCCGGCGAGCACACCATCCGGAACTACCCCGCCACCGTGCACGGCGCCCTGCTGAGC
GGCCTGCGGGAGGCAGGACGGATCGCCGACCAGTTCCTGGGCGCCATGTACACCCTGCCCC
GGCAGGCCACCCCCGGCGTGCCCGCCCAGCAGAGCCCCAGCATG (SEQ ID NO: 54) In some embodiments, LSD1 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the LSD1 sequence of SEQ ID NO: 64. In some embodiments, an LSD1 variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 64.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises LSD1 and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the LSD1 sequence of SEQ ID NO: 64, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises EZH2, e.g., as according to NP-004447.2 or the protein encoded by NM_004456.5 or a functional variant or fragment thereof. In some embodiments, EZH2 comprises an amino acid sequence of SEQ ID NO: 65:
GQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFS SNRQKILERTEILNQEWKQRRI
QPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNI
PYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEE
REEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECT
PNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ
HLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNS SRPS TPTINVLESKDTD SDREAGTETGGEN
NDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLI
GTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQP
CDHPRQPCD S SCPCVIA QNFCEKFCQC S SECQNRFPGCRC KAQCNTKQCPCYLAVRECDPDLCLT
CGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEAD
RRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKR
AIQTGEELFFDYRYSQADALKYVGIEREMEIP (SEQ ID NO: 65) In some embodiments, the EZH2 repressor domain is encoded by a nucleotide sequence of SEQ
ID NO: 55. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ
ID NO: 55 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
GGCCAGACCGGCAAGAAGAGCGAGAAGGGCCCCGTGTGCTGGCGGAAGCGGGTGAAGAGC
GAGTACATGCGGCTGCGGCAGCTGAAGCGGTTCCGGCGGGCCGACGAGGTGAAGAGCATGT
TCAGCAGCAACCGGCAGAAGATCCTGGAGCGGACCGAGATCCTGAACCAGGAGTGGAAGCA
GCGGCGAATCCAGCCCGTGCACATCCTGACCAGCGTGAGCAGCCTGCGGGGCACCCGGGAG
TGCAGCGTGACCAGCGACCTGGACTTCCCCACCCAGGTGATCCCCCTAAAGACCCTGAACGC
CGTGGCCAGCGTGCCCATCATGTACAGCTGGAGCCCCCTGCAGCAGAACTTCATGGTGGAGG
ACGAGACCGTGCTGCACAACATCCCCTACATGGGCGACGAGGTGCTGGACCAGGACGGCAC
CTTCATCGAGGAGCTGATCAAGAACTACGACGGCAAGGTGCACGGCGACCGGGAGTGCGGC
TTCATCAACGACGAGATCTTCGTGGAGCTGGTGAACGCCCTGGGCCAGTACAACGACGACG
ACGACGACGACGACGGCGACGACCCCGAGGAGCGGGAGGAGAAGCAGAAGGACCTGGAGG
ACCACCGGGACGACAAGGAGAGCCGGCCCCCCCGGAAGTTCCCCAGCGACAAGATCTTCGA
GGCCATCAGCAGCATGTTCCCCGACAAGGGCACCGCCGAGGAGCTGAAGGAGAAGTACAAG
GAGCTGACCGAGCAGCAGCTGCCCGGCGCCCTGCCCCCCGAGTGCACCCCCAACATCGACG
GCCCCAACGCCAAGAGCGTGCAGCGGGAGCAGAGCCTGCACAGCTTCCACACCCTGTTCTG
CCGGCGGTGCTTCAAGTACGACTGCTTCCTGCACCCCTTCCACGCCACCCCCAACACCTACA
AGCGGAAGAACACCGAGACCGCCCTGGACAACAAGCCCTGCGGCCCCCAGTGCTACCAGCA
CCTGGAGGGCGCCAAGGAGTTCGCCGCCGCCCTGACCGCCGAGCGGATCAAGACCCCCCCC
AAGCGGCCCGGCGGCCGGCGGCGGGGCCGGCTGCCCAACAACAGCAGCCGGCCCAGCACCC
CCACCATCAACGTGCTGGAGAGCAAGGACACCGACAGCGACCGGGAGGCCGGCACCGAGA
CCGGCGGCGAGAACAACGACAAGGAGGAGGAGGAGAAGAAGGACGAGACCAGCAGCAGC
AGCGAGGCCAACAGCCGGTGCCAGACCCCCATCAAGATGAAGCCCAACATCGAGCCCCCCG
AGAACGTGGAGTGGAGCGGCGCCGAGGCCAGCATGTTCCGGGTGCTGATCGGCACCTACTA
CGACAACTTCTGCGCCATCGCCCGGCTGATCGGCACCAAGACCTGCCGGCAGGTGTACGAGT
TCCGGGTGAAGGAGAGCAGCATCATCGCCCCCGCCCCCGCCGAGGACGTGGACACCCCCCC
CCGGAAGAAGAAGCGGAAGCACCGGCTGTGGGCCGCCCACTGCCGGAAGATCCAGCTGAAG
AAGGACGGCAGCAGCAACCACGTGTACAACTACCAGCCCTGCGACCACCCCCGGCAGCCCT
GCGACAGCAGCTGCCCCTGCGTGATCGCCCAGAACTTCTGCGAGAAGTTCTGCCAGTGCAGC
AGCGAGTGCCAGAACCGGTTCCCCGGCTGCCGGTGCAAGGCCCAGTGCAACACCAAGCAGT
GCCCCTGCTACCTGGCCGTGCGGGAGTGCGACCCCGACCTGTGCCTGACCTGCGGCGCCGCC
GACCACTGGGACAGCAAGAACGTGAGCTGCAAGAACTGCAGCATCCAGCGGGGCAGCAAG
AAGCACCTGCTGCTGGCCCCCAGCGACGTGGCCGGCTGGGGCATCTTCATCAAGGACCCCGT
GCAGAAGAACGAGTTCATCAGCGAGTACTGCGGCGAGATCATCAGCCAGGACGAGGCCGAC
CGGCGGGGCAAGGTGTACGACAAGTACATGTGCAGCTTCCTGTTCAACCTGAACAACGACTT
CGTGGTGGACGCCACCCGGAAGGGCAACAAGATCCGGTTCGCCAACCACAGCGTGAACCCC
AACTGCTACGCCAAGGTGATGATGGTGAACGGCGACCACCGGATCGGCATCTTCGCCAAGC
GGGCCATCCAGACCGGCGAGGAGCTGTTCTTCGACTACCGGTACAGCCAGGCCGACGCCCT
GAAGTACGTGGGCATCGAGCGGGAGATGGAGATCCCC (SEQ ID NO: 55) In some embodiments, EZH2 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the EZH2 sequence of SEQ ID NO: 65. In some embodiments, an EZH2 variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 65.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises EZH2 and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the EZH2 sequence of SEQ ID NO: 65, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises FOG1 e.g., as according to NP_722520.2 or the protein encoded by NM_153813.3 or a functional variant or fragment thereof. In some embodiments, FOG1 comprises an amino acid sequence of SEQ ID NO: 66:
SRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPEAPSP (SEQ ID NO: 66) In some embodiments, the FOG1 repressor domain is encoded by a nucleotide sequence of SEQ
ID NO: 56. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ
ID NO: 56 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
AGCAGAAGGAAGCAGAGCAACCCCAGACAAATCAAGAGATCTCTGGGCGACATGGAGGCC
AGAGAGGAAGTGCAGCTGGTGGGCGCCAGCCACATGGAGCAGAAGGCTACAGCCCCCGAG
GCCCCCAGCCCC (SEQ ID NO: 56) In some embodiments, FOG1 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the FOG 1 sequence of SEQ ID NO: 66.
In some embodiments, an FOG1 variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 66.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises FOG1 and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some 10 embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor 15 domain comprising the FOG1 sequence of SEQ ID NO: 66, or a functional variant or fragment thereof.
In some embodiments, provided technologies are described as comprising a gRNA
that specifically targets a target gene. In some embodiments, the target gene is an oncogene, a tumor suppressor, or a MYC mis-regulation disorder related gene. In some embodiments, the target gene is 20 MYC. In some embodiments, the target gene is an MHC class I molecule, e.g., I32M. In some embodiments, the target gene encodes a heat shock protein, e.g., HSPA1B. In some embodiments, the target gene is a transcription factor, e.g., GATAl.
In some embodiments, technologies provided herein include methods of delivering one or more 25 expression repressors or expression repression systems described herein to a subject, e.g., to a nucleus of a cell or tissue of a subject, by linking such a moiety to a DNA-targeting moiety as part of a fusion molecule.
In some embodiments, an expression repressor comprises a nuclear localization sequence (NLS).
30 In some embodiments, the expression repressor comprises an NLS, e.g., an 5V40 NLS at the N-terminus.
In some embodiments, the expression repressor comprises an NLS, e.g., a nucleoplasmin NLS at the C-terminus. In some embodiments, the expression repressor comprises a first NLS
at the N-terminus and a second NLS at the C-terminus. In some embodiments the first and the second NLS
have the same sequence. In some embodiments, the first and the second NLS have different sequences. In some embodiments, the expression repression repressor comprises an SV40 NLS, e.g., the expression repressor comprises a sequence according to PKKKRK (SEQ ID NO: 86). In some embodiments, the expression repressor comprises an epitope tag, e.g., an HA tag: YPYDVPDYA (SEQ ID NO:
80). For example, the expression repressor may comprise two copies of the epitope tag.
While an epitope tag is useful in many research contexts, it is sometimes desirable to omit an epitope tag in a therapeutic context. Accordingly, in some embodiments, the expression repressor lacks an epitope tag. In some embodiments, an expression repressor described herein comprises a sequence provided herein (or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto), but lacking the HA tag of SEQ ID NO: 80. In some embodiments, a nucleic acid described herein comprises a sequence provided herein (or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto), but lacking a region encoding the HA tag of SEQ ID NO: 80. In some embodiments, the expression repressor comprises a nucleoplasmin NLS, e.g., the expression repressor comprises a sequence of KRPAATKKAGQAKKK (SEQ ID NO: 87). In some embodiments, the expression repressor does not comprise an NLS. In some embodiments, the expression repressor does not comprise an epitope tag. In some embodiments the expression repressor does not comprise an HA tag. In some embodiments, the expression repressor does not comprise an HA tag sequence according to SEQ ID
NO: 80.
DNA-Targeting Moieties DNA-targeting moieties may specifically bind a DNA sequence, e.g., a DNA
sequence associated with a target gene, e.g., binds, a genomic sequence element (e.g., a promoter, a TSS, or an anchor sequence) in, proximal to, and/or operably linked to a target gene. Any molecule or compound that specifically binds a DNA sequence may be used as a DNA-targeting moiety.
In some embodiments, a DNA-targeting moiety targets, e.g., binds, a component of a genomic complex (e.g., ASMC). In some embodiments, a DNA-targeting moiety targets, e.g., binds, an expression control sequence (e.g., a promoter or enhancer) operably linked to a target gene. In some embodiments, a DNA-targeting moiety targets, e.g., binds, a target gene, or a part of a target gene. The target of a DNA-targeting moiety may be referred to as its targeted component. A targeted component may be any genomic sequence element operably linked to a target gene, or the target gene itself, including but not limited to a promoter, enhancer, anchor sequence, exon, intron, UTR encoding sequence, a splice site, or a transcription start site. In some embodiments, a DNA-targeting moiety binds specifically to one or more target anchor sequences (e.g., within a cell) and not to non-targeted anchor sequences (e.g., within the same cell).
In some embodiments, a DNA-targeting moiety may be or comprise a CRISPR/Cas molecule, a TAL effector molecule, a Zn finger domain, peptide nucleic acid (PNA) or a nucleic acid molecule. In some embodiments, an expression repressor comprises one DNA-targeting moiety.
In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each member of the plurality of expression repressors comprises a DNA-targeting moiety, wherein each DNA-targeting moiety does not detectably bind, e.g., does not bind, to another DNA-targeting moiety. In some embodiments, an expression repression system comprises a first expression repressor comprising a first DNA-targeting moiety and a second expression repressor comprising a second DNA-targeting moiety, wherein the first DNA-targeting moiety does not detectably bind, e.g., does not bind, to the second DNA-targeting moiety. In some embodiments, an expression repression system comprises a first expression repressor comprising a first DNA-targeting moiety and a second expression repressor comprising a second DNA-targeting moiety, wherein the first DNA-targeting moiety does not detectably bind, e.g., does not bind, to another first DNA-targeting moiety, and the second DNA-targeting moiety does not detectably bind, e.g., does not bind, to another second DNA-targeting moiety. In some embodiments, a DNA-targeting moiety for use in the compositions and methods described herein is functional (e.g., binds to a DNA sequence) in a monomeric, e.g., non-dimeric, state.
In some embodiments, binding of a targeting moiety to a targeted component decreases binding affinity of the targeted component for another transcription factor, genomic complex component, or genomic sequence element. In some embodiments, a DNA-targeting moiety binds to its target sequence with a KD of less than or equal to 500, 450, 400, 350, 300, 250, 200, 150, 100, 50, 40, 30, 20, 10, 9, 8, 7, 6, 5, 4, 3, 2, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0.09, 0.08, 0.07, 0.06, 0.05, 0.04, 0.03, 0.02, 0.01, 0.005, 0.002, or 0.001 nM (and optionally, a KD of at least 50, 40, 30, 20, 10, 9, 8, 7, 6, 5, 4, 3, 2, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0.09, 0.08, 0.07, 0.06, 0.05, 0.04, 0.03, 0.02, 0.01, 0.005, 0.002, or 0.001 nM). In some embodiments, a DNA-targeting moiety binds to its target sequence with a KD of 0.001 nM to 500 nM, e.g., 0.1 nM to 5 nM, e.g., about 0.5 nM. In some embodiments, a DNA-targeting moiety binds to a non-target sequence with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or 100,000 nM (and optionally, does not appreciably bind to a non-target sequence). In some embodiments, a DNA-targeting moiety does not bind to a non-target sequence.
In some embodiments, a DNA-targeting moiety comprises a nucleic acid sequence complementary to a targeted component, e.g., a promoter of a target gene. In some embodiments, a targeting moiety comprises a nucleic acid sequence that is at least 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100%
complementary to a targeted component.
In some embodiments, the DNA-targeting moiety of an expression repressor comprises no more than 100, 90, 80, 70, 60, 50, 40, 30, or 20 nucleotides (and optionally at least 10, 20, 30, 40, 50, 60, 70, 80, or 90 nucleotides). In some embodiments, an expression repressor or a repressor domain of a fusion molecule, comprises no more than 2000, 1900, 1800, 1700, 1600, 1500, 1400, 1300, 1200, 1100, 1000, 900, 800, 700, 600, 500, 400, 300, 200, or 100 amino acids (and optionally at least 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, or 1900 amino acids). In some embodiments, an expression repressor or the effector moiety of a fusion molecule, comprises 100-2000, 100-1900, 100-1800, 100-1700, 100-1600, 100-1500, 100-1400, 100-1300, 100-1200, 100-1100, 100-1000, 100-900, 100-800, 100-700, 100-600, 100-500, 100-400, 100-300, 100-200, 200-2000, 200-1900, 200-1800, 200-1700, 200-1600, 200-1500, 200-1400, 200-1300, 200-1200, 200-1100, 200-1000, 200-900, 200-800, 200-700, 200-600, 200-500, 200-400, 200-300, 300-2000, 300-1900, 300-1800, 300-1700, 300-1600, 300-1500, 300-1400, 300-1300, 300-1200, 300-1100, 300-1000, 300-900, 300-800, 300-700, 300-600, 300-500, 300-400, 400-2000, 400-1900, 400-1800, 400-1700, 400-1600, 400-1500, 400-1400, 400-1300, 400-1200, 400-1100, 400-1000, 400-900, 400-800, 400-700, 400-600, 400-500, 500-2000, 500-1900, 500-1800, 500-1700, 500-1600, 500-1500, 500-1400, 500-1300, 500-1200, 500-1100, 500-1000, 500-900, 500-800, 500-700, 500-600, 600-2000, 600-1900, 600-1800, 600-1700, 600-1600, 600-1500, 600-1400, 600-1300, 600-1200, 600-1100, 600-1000, 600-900, 600-800, 600-700, 700-2000, 700-1900, 700-1800, 700-1700, 700-1600, 700-1500, 700-1400, 700-1300, 700-1200, 700-1100, 700-1000, 700-900, 700-800, 800-2000, 800-1900, 800-1800, 800-1700, 800-1600, 800-1500, 800-1400, 800-1300, 800-1200, 800-1100, 800-1000, 800-900, 900-2000, 900-1900, 900-1800, 900-1700, 900-1600, 900-1500, 900-1400, 900-1300, 900-1200, 900-1100, 900-1000, 1000-2000, 1000-1900, 1000-1800, 1000-1700, 1000-1600, 1000-1500, 1000-1400, 1000-1300, 1000-1200, 1000-1100, 1100-2000, 1100-1900, 1100-1800, 1100-1700, 1100-1600, 1100-1500, 1100-1400, 1100-1300, 1100-1200, 1200-2000, 1200-1900, 1200-1800, 1200-1700, 1200-1600, 1200-1500, 1200-1400, 1200-1300, 1300-2000, 1300-1900, 1300-1800, 1300-1700, 1300-1600, 1300-1500, 1300-1400, 1400-2000, 1400-1900, 1400-1800, 1400-1700, 1400-1600, 1400-1500, 1500-2000, 1500-1900, 1500-1800, 1500-1700, 1500-1600, 1600-2000, 1600-1900, 1600-1800, 1600-1700, 1700-2000, 1700-1900, 1700-1800, 1800-2000, 1800-1900, or 1900-2000 amino acids.
An expression repressor or an expression repression system as disclosed herein, may comprise nucleic acid, e.g., one or more nucleic acids. In some embodiments, a nucleic acid is or comprises RNA;
in some embodiments, a nucleic acid is or comprises DNA. In some embodiments, a nucleic acid is or comprises more than 50% ribonucleotides. In some embodiments, a nucleic acid is, comprises, or consists of one or more natural nucleic acid residues. In some embodiments, a nucleic acid is, comprises, or consists of one or more nucleic acid analogs. In some embodiments, a nucleic acid analog differs from a nucleic acid in that it does not utilize a phosphodiester backbone. For example, in some embodiments, a nucleic acid is, comprises, or consists of one or more peptide nucleic acids.
Alternatively, or additionally, in some embodiments, a nucleic acid has one or more phosphorothioate and/or 5'-N-phosphoramidite linkages rather than phosphodiester bonds. In some embodiments, a nucleic acid is, comprises, or consists of one or more natural nucleosides (e.g., adenosine, thymidine, guanosine, cytidine, uridine, deoxyadenosine, deoxythymidine, deoxy guanosine, and deoxycytidine). In some embodiments, a nucleic .. acid is, comprises, or consists of one or more nucleoside analogs (e.g., 2-aminoadenosine, 2-thiothymidine, inosine, pyrrolo-pyrimidine, 3 -methyl adenosine, 5-methylcytidine, C-5 propynyl-cytidine, C-5 propynyl-uridine, 2-aminoadenosine, C5-bromouridine, C5-fluorouridine, C5-iodouridine, C5-propynyl-uridine, C5 -propynyl-cytidine, C5-methylcytidine, 2-aminoadenosine, 7-deazaadenosine, 7-deazaguanosine, 8-oxoadenosine, 8-oxoguanosine, 0(6)-methylguanine, 2-thiocytidine, methylated bases, intercalated bases, and combinations thereof). In some embodiments, a nucleic acid comprises one or more modified sugars (e.g., 2'-fluororibose, ribose, 2'-deoxyribose, arabinose, and hexose) as compared with those in natural nucleic acids. In some embodiments, a nucleic acid has a nucleotide sequence that encodes a functional gene product such as an RNA or protein. In some embodiments, a nucleic acid includes one or more introns. In some embodiments, nucleic acids are prepared by one or more of .. isolation from a natural source, enzymatic synthesis by polymerization based on a complementary template (in vivo or in vitro), reproduction in a recombinant cell or system, and chemical synthesis. In some embodiments, a nucleic acid is at least 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 20, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 600, 700, 800, 900, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000 or more residues long. In some embodiments, nucleic acids may have a length from about 2 to about 5000 nts, about 10 to about 100 nts, about 50 to about 150 nts, about 100 to about 200 nts, about 150 to about 250 nts, about 200 to about 300 nts, about 250 to about 350 nts, about 300 to about 500 nts, about 10 to about 1000 nts, about 50 to about 1000 nts, about 100 to about 1000 nts, about 1000 to about 2000 nts, about 2000 to about 3000 nts, about 3000 to about 4000 nts, about 4000 to about 5000 nts, or any range therebetween. In some embodiments, a nucleic acid is partly or wholly single stranded; in some embodiments, a nucleic acid is partly or wholly double stranded. In some embodiments a nucleic acid has a nucleotide sequence comprising at least one element that encodes, or is the complement of a sequence that encodes, a polypeptide. In some embodiments, a nucleic acid has enzymatic activity.
In some embodiments, a DNA-targeting moiety comprises or is nucleic acid.
In some embodiments, a nucleic acid that may be included in a moiety may be or comprise DNA, RNA, and/or an artificial or synthetic nucleic acid or nucleic acid analog or mimic. For example, in some embodiments, a nucleic acid may be or include one or more of genomic DNA
(gDNA), complementary 5 DNA (cDNA), a peptide nucleic acid (PNA), a peptide-nucleic acid mixmer, a peptide- oligonucleotide conjugate, a locked nucleic acid (LNA), a bridged nucleic acid (BNA), a polyamide, a triplex- forming oligonucleotide, an antisense oligonucleotide, tRNA, mRNA, rRNA, miRNA, gRNA, siRNA or other RNAi molecule (e.g., that targets a non-coding RNA as described herein and/or that targets an expression product of a particular gene associated with a targeted genomic complex as described herein), etc. A
10 nucleic acid sequence may include modified oligonucleotides (e.g., chemical modifications, such as modifications that alter backbone linkages, sugar molecules, and/or nucleic acid bases) and/or artificial nucleic acids. In some embodiments, a nucleic acid sequence includes, but is not limited to, genomic DNA, cDNA, peptide nucleic acids (PNA) or peptide oligonucleotide conjugates, locked nucleic acids (LNA), bridged nucleic acids (BNA), polyamides, triplex forming oligonucleotides, modified DNA, 15 antisense DNA oligonucleotides, tRNA, mRNA, rRNA, modified RNA, miRNA, gRNA, and siRNA or other RNA or DNA molecules. In some embodiments, a nucleic acid may include one or more residues that is not a naturally occurring DNA or RNA residue, may include one or more linkages that is/are not phosphodiester bonds (e.g., that may be, for example, phosphorothioate bonds, etc.), and/or may include one or more modifications such as, for example, a 2'0 modification such as 2'-0MeP. A variety of 20 nucleic acid structures useful in preparing synthetic nucleic acids is known in the art (see, for example, W02017/0628621 and W02014/012081) those skilled in the art will appreciate that these may be utilized in accordance with the present disclosure.
In some embodiments, a nucleic acid described herein comprises one or more nucleoside analogs.
In some embodiments, a nucleic acid sequence may include in addition or as an alternative to one or more 25 natural nucleosides, e.g., purines or pyrimidines, e.g., adenine, cytosine, guanine, thymine, and uracil, one or more nucleoside analogs. In some embodiments, a nucleic acid sequence includes one or more nucleoside analogs. A nucleoside analog may include, but is not limited to, a nucleoside analog, such as 5-fluorouracil; 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 4-methylbenzimidazole, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethy1-2-thiouridine, 5-30 carboxymethylaminomethyluracil, dihydrouracil, dihydrouridine, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethy1-2-thiouracil, beta-D-mannosylqueosine, 5'-methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v), 5-methy1-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w, 2,6-diaminopurine, 3-nitropyrrole, inosine, thiouridine, queuosine, wyosine, diaminopurine, isoguanine, isocytosine, diaminopyrimidine, 2,4-difluorotoluene, isoquinoline, pyrrolo[2,3-13]pyridine, and any others that can base pair with a purine or a pyrimidine side chain.
CRISPR/Cas Domains In some embodiments, a DNA-targeting moiety is or comprises a CRISPR/Cas molecule. A
CRISPR/Cas molecule comprises a protein involved in the clustered regulatory interspaced short palindromic repeat (CRISPR) system, e.g., a Cas protein, and optionally a guide RNA, e.g., single guide RNA (sgRNA). In some embodiments, the gRNA comprised by the CRISPR/Cas molecule is noncovalently bound by the CRISPR/Cas protein.
CRISPR systems are adaptive defense systems originally discovered in bacteria and archaea.
CRISPR systems use RNA-guided nucleases termed CRISPR-associated or "Cas"
endonucleases (e. g., Cas9 or Cpfl) to cleave foreign DNA. For example, in a typical CRISPR/Cas system, an endonuclease is directed to a target nucleotide sequence (e. g., a site in the genome that is to be sequence-edited) by sequence-specific, non-coding "guide RNAs" that target single- or double-stranded DNA sequences.
Three classes (I-III) of CRISPR systems have been identified. The class II
CRISPR systems use a single Cas endonuclease (rather than multiple Cas proteins). One class II CRISPR
system includes a type II Cas endonuclease such as Cas9, a CRISPR RNA ("crRNA"), and a trans-activating crRNA ("tracrRNA").
The crRNA contains a "guide RNA", typically about 20-nucleotide RNA sequence that corresponds to a target DNA sequence. crRNA also contains a region that binds to the tracrRNA
to form a partially double-stranded structure which is cleaved by RNase III, resulting in a crRNA/tracrRNA hybrid. A
.. crRNA/tracrRNA hybrid then directs Cas9 endonuclease to recognize and cleave a target DNA sequence.
A target DNA sequence must generally be adjacent to a "protospacer adjacent motif' ("PAM") that is specific for a given Cas endonuclease; however, PAM sequences appear throughout a given genome.
CRISPR endonucleases identified from various prokaryotic species have unique PAM sequence requirements; examples of PAM sequences include 5'-NGG (Streptococcus pyogenes), 5'-NNAGAA
(Streptococcus thermophilus CRISPR1), 5' -NGGNG (Streptococcus thermophilus CRISPR3), and 5'-NNNGATT (Neisseria meningiditis). Some endonucleases, e.g., Cas9 endonucleases, are associated with G-rich PAM sites, e. g., 5'-NGG, and perform blunt-end cleaving of the target DNA at a location 3 nucleotides upstream from (5' from) the PAM site. Another class II CRISPR
system includes the type V
endonuclease Cpfl, which is smaller than Cas9; examples include AsCpfl (from Acidaminococcus sp.) and LbCpfl (from Lachnospiraceae sp.). Cpfl-associated CRISPR arrays are processed into mature crRNAs without the requirement of a tracrRNA; in other words, a Cpfl system requires only Cpfl nuclease and a crRNA to cleave a target DNA sequence. Cpfl endonucleases, are associated with T-rich PAM sites, e. g., 5'-TTN. Cpfl can also recognize a 5'-CTA PAM motif. Cpfl cleaves a target DNA by introducing an offset or staggered double-strand break with a 4- or 5-nucleotide 5' overhang, for example, cleaving a target DNA with a 5-nucleotide offset or staggered cut located 18 nucleotides downstream from (3' from) from a PAM site on the coding strand and 23 nucleotides downstream from the PAM site on the complimentary strand; the 5-nucleotide overhang that results from such offset cleavage allows more precise genome editing by DNA insertion by homologous recombination than by insertion at blunt-end cleaved DNA. See, e.g., Zetsche et al. (2015) Cell, 163:759 ¨ 771.
A variety of CRISPR associated (Cas) genes or proteins can be used in the technologies provided by the present disclosure and the choice of Cas protein will depend upon the particular conditions of the method. Specific examples of Cas proteins include class II systems including Casl, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9, Cas10, Cpfl, C2C1, or C2C3. In some embodiments, a Cas protein, e.g., a Cas9 protein, may be from any of a variety of prokaryotic species. In some embodiments a particular Cas protein, e.g., a particular Cas9 protein, is selected to recognize a particular protospacer-adjacent motif (PAM) sequence. In some embodiments, a DNA-targeting moiety includes a sequence targeting polypeptide, such as a Cas protein, e.g., Cas9. In certain embodiments a Cas protein, e.g., a Cas9 protein, may be obtained from a bacteria or archaea or synthesized using known methods.
In certain embodiments, a Cas protein may be from a gram-positive bacterium or a gram-negative bacterium. In certain embodiments, a Cas protein may be from a Streptococcus (e.g., S. pyogenes, or a S. thermophilus), a Francisella (e.g., an F. novicida), a Staphylococcus (e.g., an S. aureus), an Acidaminococcus (e.g., an Acidaminococcus sp. BV3L6), a Neisseria (e.g., an N. meningitidis), a Cryptococcus, a Corynebacterium, a Haemophilus, a Eubacterium, a Pasteurella, a Prevotella, a Veillonella, or a Marinobacter.
.. In some embodiments, a Cas protein requires a protospacer adjacent motif (PAM) to be present in or adjacent to a target DNA sequence for the Cas protein to bind and/or function.
In some embodiments, the PAM is or comprises, from 5' to 3', NGG, YG, NNGRRT, NNNRRT, NGA, TYCV, TATV, NTTN, or NNNGATT, where N stands for any nucleotide, Y stands for C or T, R stands for A or G, and V stands for A or C or G. In some embodiments, a Cas protein is a protein listed in Table 1. In some embodiments, a Cas protein comprises one or more mutations altering its PAM.
In some embodiments, a Cas protein comprises E1369R, E1449H, and R1556A mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a Cas protein comprises E782K, N968K, and R1015H mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a Cas protein comprises D1135V, R1335Q, and T1337R mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a Cas protein comprises S542R and K607R mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a Cas protein comprises S542R, K548V, and N552R mutations or analogous substitutions to the amino acids corresponding to said positions.
Table 1 Name Enzym Species # of PAM Mutations to alter Mutations to make AAs PAM recognition catalytically dead FnCas Cas9 Francisella 1629 5'-NGG- Wt 9 novicida 3' FnCas Cas9 Francisella 1629 5'-YG-3' E1369R/E1449H/R1 D11A/H969A/N995A
9 RHA novicida 556A
SaCas Cas9 Staphylococc 1053 5'- Wt D1OA/H557A
9 us aureus NNGRR
T-3' SaCas Cas9 Staphylococc 1053 5'- E782K/N968K/R10 D1OA/H557A
9 us aureus NNNRR 15H
KKH T-3' SpCas Cas9 Streptococcu 1368 5'-NGG- Wt 9 s pyogenes 3' N863A
SpCas Cas9 Streptococcu 1368 5'-NGA- D1135V/R1335Q/T D1OA/D839A/H840A/
9 VQR s pyogenes 3' 1337R N863A
AsCpf Cpfl Acidaminoco 1307 5'-TYCV- S542R/K607R E993A
1 RR ccus sp. 3' AsCpf Cpfl Acidaminoco 1307 5'-TATV- S542R/K548V/N552 E993A
1 RVR ccus sp. 3' FnCpf Cpfl Francisella 1300 5'-NTTN- Wt 1 novicida 3' A
NmCa Cas9 Neisseria 1082 5'- Wt s9 meningitidis NNNGA N611A
TT-3' In some embodiments, the Cas protein is modified to deactivate the nuclease, e.g., nuclease deficient Cas9. Whereas wild-type Cas9 generates double-strand breaks (DSBs) at specific DNA
sequences targeted by a gRNA, a number of CRISPR endonucleases having modified functionalities are available, for example: a "nickase" version of Cas9 generates only a single-strand break; a catalytically inactive Cas9 ("dCas9") does not cut target DNA. In some embodiments, dCas9 binding to a DNA
sequence may interfere with transcription at that site by steric hindrance. In some embodiments, a DNA-targeting moiety is or comprises a catalytically inactive Cas9, e.g., dCas9.
In some embodiments, a DNA-targeting moiety is or comprises a catalytically inactive mutant Cas9, e.g., Cas9m4. Many catalytically inactive Cas9 proteins are known in the art. In some embodiments, dCas9 comprises mutations in each endonuclease domain of the Cas protein, e.g., DlOA and H840A
mutations.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a DllA
mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a H969A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a N995A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises D11A, H969A, and N995A mutations or analogous substitutions to the amino acids corresponding to said positions.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a DlOA
mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a H557A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises DlOA and H557A
mutations or analogous substitutions to the amino acids corresponding to said positions.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D839A
mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a H840A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a N863A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises DlOA and D839A mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises DlOA, D839A, and H840A mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises DlOA, D839A, H840A, and N863A mutations or analogous substitutions to the amino acids corresponding to said positions.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a E993A
mutation or an analogous substitution to the amino acid corresponding to said position.
5 In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D917A
mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a E1006A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D1255A mutation or an analogous 10 substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises D917A, E1006A, and D1255A
mutations or analogous substitutions to the amino acids corresponding to said positions.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D16A
mutation or an analogous substitution to the amino acid corresponding to said position. In some 15 embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D587A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a H588A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a N611A mutation or an analogous substitution to the amino acid 20 corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises D16A, D587A, H588A, and N611A mutations or analogous substitutions to the amino acids corresponding to said positions.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, wherein the 25 one or more DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a Cas protein, e.g., catalytically inactive Cas9 protein, e.g., dCas9, e.g., dCas9m4, or a functional variant or fragment thereof. In some embodiments, dCas9 comprises an amino acid sequence of SEQ ID
NO: 46 or 88:
DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEA
TRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVA
NQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDL
AEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIK
RYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEE
LLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPL
ARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFT
VYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGV
EDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMK
QLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSR
ERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD INRLSDYDVAAIV
PQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAER
GGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDF
QFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKAT
AKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWD KGRDFATVRKVLSMPQVNIV KKTE
V QTGGFSKESILPKRNS D KLIARKKDWDPKKYGGFD SPTVAYSVLVVAKVEKGKSKKLKSVKEL
LGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLASAGELQKGNELALP
SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAY
NKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRID
LSQLGGD (SEQ ID NO: 46) AKRNYILGLAIGIT SVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRS KRGARRLKRRR
RHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLS QKLSEEEFSAALLHLAKRRGVHNVNEVE
EDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKA
YHQLD QSFIDTYIDLLETRRTYYEGPGEGS PFGWKDIKEWYEMLMGHCTYFPEELRSV KYAYNA
DLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTG
KPEFTNLKVYHDIKDITARKEIIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKG
YTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFI
QS IKVINAIIKKYGLPND IIIELAREKNS KDAQKMINEMQKRNRQTNERIEEIIRTTGKENAKYLIEK
IKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDAIIPRS VS FDNS FNNKVLVKQEENSKKGNRTPF
QYLSS SD S KISYETFKKHILNLAKGKGRIS KTKKEYLLEERDINRFSV QKD FINRNLVDTRYATRG
LMNLLRS YFRVNNLDVKV KS INGGFTS FLRRKWKFKKERNKGYKHHAEDALIIANADFIFKEWK
KLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQIKHIKDFKDYKYSHRVDKKPNRKLI
NDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQY
GDEKNPLYKYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKP
YRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYKNDLIKIN
GELYRVIGVNNDLLNRIEVNMIDITYREYLENMNDKRPPHIIKTIASKTQSIKKYSTDILGNLYEVK
SKKHPQIIKKG (SEQ ID NO: 88) In some embodiments, the dCas9 is encoded by a nucleic acid sequence of SEQ ID
NO: 45 or 89:
GACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCA
CCGACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAG
CATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACC
CGGCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGC
AGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGA
GAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTG
GACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGG
ACAGCACCGACAAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTC
CGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGT
TCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGG
CGTGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTG
ATCGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCC
TGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCT
GAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTAC
GCCGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCG
GGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAG
CACCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACA
AGGAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAG
CCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAG
CTGCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCA
GCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTC
TACCCCTTCCTGAAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTA
CTACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAG
GAGACCATCACCCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCT
TCATCGAGCGGATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCA
CAGCCTGCTGTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCG
AGGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCT
GTTCAAGACCAACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATC
GAGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCA
CCTACCACGACCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGA
GGACATCCTGGAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAG
GAGCGGCTGAAAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGC
GGCGGTACACCGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCA
GAGCGGCAAGACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGC
AGCTGATCCACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGG
CCAGGGCGACAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAG
GGCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGC
CCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGA
ACAGCCGGGAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCC
TGAAGGAGCACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCT
GCAGAACGGCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTAC
GACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGC
TGACCCGGAGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGA
AGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTT
CGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATC
AAGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCC
GGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCT
GAAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATC
AACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCA
AGAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCG
GAAGATGATCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTAC
AGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGC
GGCCCCTGATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTT
CGCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTG
CAGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCG
CCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTA
CAGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAA
GGAGCTGCTGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTC
CTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACA
GCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAA
GGGCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACG
AGAAGCTGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACA
AGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGC
CGACGCCAACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGG
GAGCAGGCCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTT
CAAGTACTTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGAC
GCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCT
GGGCGGCGAC (SEQ ID NO: 45) GCCAAGCGGAACTACATCCTGGGCCTGGCCATCGGCATCACCAGCGTGGGCTACGGCATCAT
CGACTACGAGACCCGGGACGTGATCGACGCCGGCGTGCGGCTGTTCAAGGAGGCCAACGTG
GAGAACAACGAGGGCCGGCGGAGCAAGCGGGGCGCCCGGCGGCTGAAGCGGCGGCGGCGG
CACCGGATCCAGCGGGTGAAGAAGCTGCTGTTCGACTACAACCTGCTGACCGACCACAGCG
AGCTGAGCGGCATCAACCCCTACGAGGCCCGGGTGAAGGGCCTGAGCCAGAAGCTGAGCGA
GGAGGAGTTCAGCGCCGCCCTGCTGCACCTGGCCAAGCGGCGGGGCGTGCACAACGTGAAC
GAGGTGGAGGAGGACACCGGCAACGAGCTGAGCACCAAGGAGCAGATCAGCCGGAACAGC
AAGGCCCTGGAGGAGAAGTACGTGGCCGAGCTGCAGCTGGAGCGGCTGAAGAAGGACGGC
GAGGTGCGGGGCAGCATCAACCGGTTCAAGACCAGCGACTACGTGAAGGAGGCCAAGCAGC
TGCTGAAGGTGCAGAAGGCCTACCACCAGCTGGACCAGAGCTTCATCGACACCTACATCGA
CCTGCTGGAGACCCGGCGGACCTACTACGAGGGCCCCGGCGAGGGCAGCCCCTTCGGCTGG
AAGGACATCAAGGAGTGGTACGAGATGCTGATGGGCCACTGCACCTACTTCCCCGAGGAGC
TGCGGAGCGTGAAGTACGCCTACAACGCCGACCTGTACAACGCCCTGAACGACCTGAACAA
CCTGGTGATCACCCGGGACGAGAACGAGAAGCTGGAGTACTACGAGAAGTTCCAGATCATC
GAGAACGTGTTCAAGCAGAAGAAGAAGCCCACCCTGAAGCAGATCGCCAAGGAGATCCTGG
TGAACGAGGAGGACATCAAGGGCTACCGGGTGACCAGCACCGGCAAGCCCGAGTTCACCAA
CCTGAAGGTGTACCACGACATCAAGGACATCACCGCCCGGAAGGAGATCATCGAGAACGCC
GAGCTGCTGGACCAGATCGCCAAGATCCTGACCATCTACCAGAGCAGCGAGGACATCCAGG
AGGAGCTGACCAACCTGAACAGCGAGCTGACCCAGGAGGAGATCGAGCAGATCAGCAACCT
GAAGGGCTACACCGGCACCCACAACCTGAGCCTGAAGGCCATCAACCTGATCCTGGACGAG
CTGTGGCACACCAACGACAACCAGATCGCCATCTTCAACCGGCTGAAGCTGGTGCCCAAGA
AGGTGGACCTGAGCCAGCAGAAGGAGATCCCCACCACCCTGGTGGACGACTTCATCCTGAG
CCCCGTGGTGAAGCGGAGCTTCATCCAGAGCATCAAGGTGATCAACGCCATCATCAAGAAG
TACGGCCTGCCCAACGACATCATCATCGAGCTGGCCCGGGAGAAGAACAGCAAGGACGCCC
AGAAGATGATCAACGAGATGCAGAAGCGGAACCGGCAGACCAACGAGCGGATCGAGGAGA
TCATCCGGACCACCGGCAAGGAGAACGCCAAGTACCTGATCGAGAAGATCAAGCTGCACGA
CATGCAGGAGGGCAAGTGCCTGTACAGCCTGGAGGCCATCCCCCTGGAGGACCTGCTGAAC
AACCCCTTCAACTACGAGGTGGACGCCATCATCCCCCGGAGCGTGAGCTTCGACAACAGCTT
CAACAACAAGGTGCTGGTGAAGCAGGAGGAGAACAGCAAGAAGGGCAACCGGACCCCCTT
CCAGTACCTGAGCAGCAGCGACAGCAAGATCAGCTACGAGACCTTCAAGAAGCACATCCTG
AACCTGGCCAAGGGCAAGGGCCGGATCAGCAAGACCAAGAAGGAGTACCTGCTGGAGGAG
CGGGACATCAACCGGTTCAGCGTGCAGAAGGACTTCATCAACCGGAACCTGGTGGACACCC
GGTACGCCACCCGGGGCCTGATGAACCTGCTGCGGAGCTACTTCCGGGTGAACAACCTGGA
CGTGAAGGTGaaatccATCAACGGCGGCTTCACCAGCTTCCTGCGGCGGAAGTGGAAGTTCAAG
AAGGAGCGGAACAAGGGCTACAAGCACCACGCCGAGGACGCCCTGATCATCGCCAACGCCG
ACTTCATCTTCAAGGAGTGGAAGAAGCTGGACAAGGCCAAGAAGGTGATGGAGAACCAGAT
GTTCGAGGAGAAGCAGGCCGAGAGCATGCCCGAGATCGAGACCGAGCAGGAGTACAAGGA
GATCTTCATCACCCCCCACCAGATCAAGCACATCAAGGACTTCAAGGACTACAAGTACAGCC
ACCGGGTGGACAAGAAGCCCAACCGGAAGCTGATCAACGACACCCTGTACAGCACCCGGAA
GGACGACAAGGGCAACACCCTGATCGTGAACAACCTGAACGGCCTGTACGACAAGGACAAC
GACAAGCTGAAGAAGCTGATCAACAAGAGCCCCGAGAAGCTGCTGATGTACCACCACGACC
CCCAGACCTACCAGAAGCTGAAGCTGATCATGGAGCAGTACGGCGACGAGAAGAACCCCCT
GTACAAGTACTACGAGGAGACCGGCAACTACCTGACCAAGTACAGCAAGAAGGACAACGGC
CCCGTGATCAAGAAGATCAAGTACTACGGCAACAAGCTGAACGCCCACCTGGACATCACCG
ACGACTACCCCAACAGCCGGAACAAGGTGGTGAAGCTGAGCCTGAAGCCCTACCGGTTCGA
CGTGTACCTGGACAACGGCGTGTACAAGTTCGTGACCGTGAAGAACCTGGACGTGATCAAG
AAGGAGAACTACTACGAGGTGAACAGCAAGTGCTACGAGGAGGCCAAGAAGCTGAAGAAG
ATCAGCAACCAGGCCGAGTTCATCGCCAGCTTCTACAAGAACGACCTGATCAAGATCAACG
GCGAGCTGTACCGGGTGATCGGCGTGAACAACGACCTGCTGAACCGGATCGAGGTGAACAT
GATCGACATCACCTACCGGGAGTACCTGGAGAACATGAACGACAAGCGGCCCCCCCACATC
ATCAAGACCATCGCCAGCAAGACCCAGAGCATCAAGAAGTACAGCACCGACATCCTGGGCA
ACCTGTACGAGGTGaaatccAAGAAGCACCCCCAGATCATCAAGAAGGGC (SEQ ID NO: 89) Guide RNA (gRNA) In some embodiments, a DNA-targeting moiety may comprise a Cas molecule comprising or linked (e.g., covalently) to a gRNA. A gRNA is a short synthetic RNA composed of a "scaffold" sequence necessary for Cas-protein binding and a user-defined ¨20 nucleotide targeting sequence for a genomic target. In practice, guide RNA sequences are generally designed to have a length of between 17 ¨ 24 nucleotides (e.g., 19, 20, or 21 nucleotides) and be complementary to the targeted nucleic acid sequence. Custom gRNA generators and algorithms are available commercially for use in the design of effective guide RNAs. Gene editing has also been achieved using a chimeric "single guide RNA"
("sgRNA"), an engineered (synthetic) single RNA molecule that mimics a naturally occurring crRNA-tracrRNA complex and contains both a tracrRNA (for binding the nuclease) and at least one crRNA
(to guide the nuclease to the sequence targeted for editing). Chemically modified sgRNAs have also been demonstrated to be effective for use with Cas proteins; see, for example, Hendel et al. (2015) Nature Biotechnol., 985 ¨991.
In some embodiments, a gRNA comprises a nucleic acid sequence that is complementary to a DNA sequence associated with a target gene. In some embodiments, the DNA
sequence is, comprises, or overlaps an expression control element that is operably linked to the target gene. In some embodiments, a gRNA comprises a nucleic acid sequence that is at least 90, 95, 99, or 100%
complementary to a DNA
sequence associated with a target gene. In some embodiments, a gRNA for use with a DNA-targeting moiety that comprises a Cas molecule is an sgRNA.
In some embodiments, a gRNA for use with a CRISPR/Cas molecule specifically binds a target sequence associated with I3-2-microglobulin expression. Such a gRNA may comprise a target-binding sequence selected from:
GD-28228: TCTCCTTGGTGGCCCGCCGT (SEQ ID NO: 1), GD-28229: GTCCCAAAGGCGCGGCGCTG (SEQ ID NO: 2), GD-28171: CCCTGCTCCCCGCCGAAAGGG (SEQ ID NO: 3), GD-28172: CTCTGGCTCCCCCAGCGCAGC (SEQ ID NO: 4), or GD-28173: GTGAACGCGTGGAGGGGCGCT (SEQ ID NO: 5).
In some embodiments, a gRNA for use with a CRISPR/Cas domain specifically binds a target sequence associated with CTCF. In some embodiments, a gRNA for use with a CRISPR/Cas domain specifically binds a target sequence associated with the promoter. In some embodiments the gRNA binds a target sequence listed in Table 3.
Table 3: Exemplary gRNA sequences Guide Target Target sequence Genomic SEQ ID NO:
Coordinates GD- MYC chr8:128746082- 11 GD- MYC chr8:128746177- 12 GD- I32M chr15:45003632- 13 GD- I32M chr15:45003545- 14 GD- HSPA1B chr6:31795352- 17 GD- HSPA1B chr6:31795429- 18 GD- GATA1 chrX:48641126- 19 GD- GATA1 chrX:48641245- 20 GD- I32M GGCGCGCACCCCAGATCGGA chr15 :45003481- 21 29499 promoter 45003503 In some embodiments, an expression repressor system comprises a first expression repressor comprising a first DNA-targeting moiety and a second expression repressor comprising a second DNA-targeting moiety, wherein the first DNA-targeting moiety comprises or is a first CRISPR/Cas molecule and the second DNA-targeting moiety comprises or is a second CRISPR/Cas molecule. In some embodiments, the first CRISPR/Cas molecule comprises a first CRISPR/Cas protein and first guide RNA, and the second CRISPR/Cas molecule comprises a second CRISPR/Cas protein and a second guide RNA.
In some embodiments, the first CRISPR/Cas protein does not appreciably bind (e.g., does not bind) the second guide RNA, e.g., binds with a KD of at least 10, 20, 50, 100, 1000, or 10,000 nM, and the second .. CRISPR/Cas protein does not appreciably bind (e.g., does not bind) the first guide RNA, e.g., binds with a KD of at least 10, 20, 50, 100, 1000, or 10,000 nM.
TAL effector domains In some embodiments, a DNA-targeting moiety is or comprises a TAL effector molecule. A TAL
effector molecule, e.g., a TAL effector molecule that specifically binds a DNA
sequence, comprises a plurality of TAL effector domains or fragments thereof, and optionally one or more additional portions of naturally occurring TAL effectors (e.g., N- and/or C-terminal of the plurality of TAL effector domains).
Many TAL effectors are known to those of skill in the art and are commercially available, e.g., from Thermo Fisher Scientific.
TALEs are natural effector proteins secreted by numerous species of bacterial pathogens including the plant pathogen Xanthomonas which modulates gene expression in host plants and facilitates bacterial colonization and survival. The specific binding of TAL effectors is based on a central repeat domain of tandemly arranged nearly identical repeats of typically 33 or 34 amino acids (the repeat-variable di-residues, RVD domain).
Members of the TAL effectors family differ mainly in the number and order of their repeats. The number of repeats ranges from 1.5 to 33.5 repeats and the C-terminal repeat is usually shorter in length (e.g., about 20 amino acids) and is generally referred to as a "half-repeat".
Each repeat of the TAL
effector feature a one-repeat-to-one-base-pair correlation with different repeat types exhibiting different base-pair specificity (one repeat recognizes one base-pair on the target gene sequence). Generally, the smaller the number of repeats, the weaker the protein-DNA interactions. A
number of 6.5 repeats has been shown to be sufficient to activate transcription of a reporter gene (Scholze et al., 2010).
Repeat to repeat variations occur predominantly at amino acid positions 12 and 13, which have therefore been termed "hypervariable" and which are responsible for the specificity of the interaction with the target DNA promoter sequence, as shown in Table 2 listing exemplary repeat variable di-residues (RVD) and their correspondence to nucleic acid base targets.
Table 2 ¨ RVDs and Nucleic Acid Base Specificity Target Possible RVD Amino Acid Combinations A NI NN CI HI KI
NN GN SN VN LN DN QN EN HN RH NK AN FN
HD RD KD ND AD
NG HG VG IG EG MG YG AA EP VA QG KG RG
Accordingly, it is possible to modify the repeats of a TAL effector to target specific DNA sequences.
Further studies have shown that the RVD NK can target G. Target sites of TAL
effectors also tend to include a T flanking the 5' base targeted by the first repeat, but the exact mechanism of this recognition is not known. More than 113 TAL effector sequences are known to date. Non-limiting examples of TAL
effectors from Xanthomonas include, Hax2, Hax3, Hax4, AvrXa7, AvrXa10 and AvrB
s3.
Accordingly, the TAL effector domain of the TAL effector molecule of the present invention may be derived from a TAL effector from any bacterial species (e.g., Xanthomonas species such as the African strain of Xanthomonas myzae pv. Oiyzae (Yu et al. 2011), Xanthomonas campestris pv. raphani strain 756C and Xanthomonas myzae pv. olyzico/astrain BL5256 (Bogdanove et al. 2011).
As used herein, the TAL effector domain in accordance with the present invention comprises an RVD
domain as well as flanking sequence(s) (sequences on the N-terminal and/or C-terminal side of the RVD domain) also from the naturally occurring TAL effector. It may comprise more or fewer repeats than the RVD of the naturally occurring TAL effector. The TAL effector molecule of the present invention is designed to target a given DNA sequence based on the above code and others known in the art. The number of TAL
effector domains (e.g., repeats (monomers or modules)) and their specific sequence are selected based on the desired DNA target sequence. For example, TAL effector domains, e.g., repeats, may be removed or added in order to suit a specific target sequence. In an embodiment, the TAL
effector molecule of the present invention comprises between 6.5 and 33.5 TAL effector domains, e.g., repeats. In an embodiment, TAL effector molecule of the present invention comprises between 8 and 33.5 TAL effector domains, e.g., repeats, e.g., between 10 and 25 TAL effector domains, e.g., repeats, e.g., between 10 and 14 TAL
effector domains, e.g., repeats.
In some embodiments, the TAL effector molecule comprises TAL effector domains that correspond to a perfect match to the DNA target sequence. In some embodiments, a mismatch between a repeat and a target base-pair on the DNA target sequence is permitted as along as it allows for the function of the expression repression system, e.g., the expression repressor comprising the TAL effector molecule. In general, TALE binding is inversely correlated with the number of mismatches. In some embodiments, the TAL effector molecule of an expression repressor of the present invention comprises no more than 7 mismatches, 6 mismatches, 5 mismatches, 4 mismatches, 3 mismatches, 2 mismatches, or 1 mismatch, and optionally no mismatch, with the target DNA sequence. Without wishing to be bound by theory, in general the smaller the number of TAL effector domains in the TAL
effector molecule, the smaller the number of mismatches will be tolerated and still allow for the function of the expression repression system, e.g., the expression repressor comprising the TAL effector molecule. The binding affinity is thought to depend on the sum of matching repeat-DNA combinations.
For example, TAL
effector molecules having 25 TAL effector domains or more may be able to tolerate up to 7 mismatches.
In addition to the TAL effector domains, the TAL effector molecule of the present invention may comprise additional sequences derived from a naturally occurring TAL effector.
The length of the C-terminal and/or N-terminal sequence(s) included on each side of the TAL
effector domain portion of the TAL effector molecule can vary and be selected by one skilled in the art, for example based on the studies of Zhang et al. (2011). Zhang et al., have characterized a number of C-terminal and N-terminal truncation mutants in Hax3 derived TAL-effector based proteins and have identified key elements, which contribute to optimal binding to the target sequence and thus activation of transcription. Generally, it was found that transcriptional activity is inversely correlated with the length of N-terminus. Regarding the C-terminus, an important element for DNA binding residues within the first 68 amino acids of the Hax 3 sequence was identified. Accordingly, in some embodiments, the first 68 amino acids on the C-terminal side of the TAL
effector domains of the naturally occurring TAL effector is included in the TAL effector molecule of an expression repressor of the present invention. Accordingly, in an embodiment, a TAL effector molecule of the present invention comprises 1) one or more TAL effector domains derived from a naturally occurring TAL effector; 2) at least 70, 80, 90, 100, 110, 120, 130, 140, 150, 170, 180, 190, 200, 220, 230, 240, 250, 260, 270, 280 or more amino acids from the naturally occurring TAL
effector on the N-terminal side of the TAL effector domains; and/or 3) at least 68, 80, 90, 100, 110, 120, 130, 140, 150, 170, 180, 190, 200, 220, 230, 240, 250, 260 or more amino acids from the naturally occurring TAL effector on the C-terminal side of the TAL effector.
Zn finger domains In some embodiments, a DNA-targeting moiety is or comprises a Zn finger domain. A Zn finger domain comprises a Zn finger protein, e.g., a naturally occurring Zn finger protein or engineered Zn finger protein, or fragment thereof. Many Zn finger proteins are known to those of skill in the art and are commercially available, e.g., from Sigma-Aldrich.
In some embodiments, a Zn finger domain comprises a non-naturally occurring Zn finger protein that is engineered to bind to a target DNA sequence of choice. See, for example, Beerli, et al. (2002) Nature Biotechnol. 20:135-141; Pabo, et al. (2001) Ann. Rev. Biochem. 70:313-340; Isalan, et al. (2001) Nature Biotechnol. 19:656-660; Segal, et al. (2001) Curr. Opin. Biotechnol.
12:632-637; Choo, et al.
(2000) Curr. Opin. Struct. Biol. 10:411-416; U.S. Pat. Nos. 6,453,242;
6,534,261; 6,599,692; 6,503,717;
6,689,558; 7,030,215; 6,794,136; 7,067,317; 7,262,054; 7,070,934; 7,361,635;
7,253,273; and U.S. Patent Publication Nos. 2005/0064474; 2007/0218528; 2005/0267061, all incorporated herein by reference in their entireties.
An engineered Zn finger protein may have a novel binding specificity, compared to a naturally .. occurring Zn finger protein. Engineering methods include, but are not limited to, rational design and various types of selection. Rational design includes, for example, using databases comprising triplet (or quadruplet) nucleotide sequences and individual Zn finger amino acid sequences, in which each triplet or quadruplet nucleotide sequence is associated with one or more amino acid sequences of zinc fingers which bind the particular triplet or quadruplet sequence. See, for example, U.S. Pat. Nos. 6,453,242 and 6,534,261, incorporated by reference herein in their entireties.
Exemplary selection methods, including phage display and two-hybrid systems, are disclosed in U.S. Pat. Nos. 5,789,538; 5,925,523; 6,007,988; 6,013,453; 6,410,248;
6,140,466; 6,200,759; and 6,242,568; as well as International Patent Publication Nos. WO 98/37186; WO
98/53057; WO 00/27878;
and WO 01/88197 and GB 2,338,237. In addition, enhancement of binding specificity for zinc finger .. proteins has been described, for example, in International Patent Publication No. WO 02/077227.
In addition, as disclosed in these and other references, zinc finger domains and/or multi-fingered zinc finger proteins may be linked together using any suitable linker sequences, including for example, linkers of 5 or more amino acids in length. See, also, U.S. Pat. Nos.
6,479,626; 6,903,185; and 7,153,949 for exemplary linker sequences 6 or more amino acids in length. The proteins described herein may .. include any combination of suitable linkers between the individual zinc fingers of the protein. In addition, enhancement of binding specificity for zinc finger binding domains has been described, for example, in co-owned International Patent Publication No. WO 02/077227.
Zn finger proteins and methods for design and construction of fusion proteins (and polynucleotides encoding same) are known to those of skill in the art and described in detail in U.S. Pat.
Nos. 6,140,0815; 789,538; 6,453,242; 6,534,261; 5,925,523; 6,007,988;
6,013,453; and 6,200,759;
International Patent Publication Nos. WO 95/19431; WO 96/06166; WO 98/53057;
WO 98/54311; WO
00/27878; WO 01/60970; WO 01/88197; WO 02/099084; WO 98/53058; WO 98/53059; WO
98/53060;
WO 02/016536; and WO 03/016496.
In addition, as disclosed in these and other references, Zn finger proteins and/or multi-fingered Zn finger proteins may be linked together, e.g., as a fusion protein, using any suitable linker sequences, including for example, linkers of 5 or more amino acids in length. See, also, U.S. Pat. Nos. 6,479,626;
6,903,185; and 7,153,949 for exemplary linker sequences 6 or more amino acids in length. The Zn finger domains described herein may include any combination of suitable linkers between the individual zinc finger proteins and/or multi-fingered Zn finger proteins of the Zn finger domain.
In certain embodiments, the DNA-targeting moiety comprises a Zn finger domain comprising an engineered zinc finger protein that binds (in a sequence-specific manner) to a target DNA sequence. In some embodiments, the Zn finger domain comprises one Zn finger protein or fragment thereof. In other embodiments, the Zn finger domain comprises a plurality of Zn finger proteins (or fragments thereof), e.g., 2, 3, 4, 5, 6 or more Zn finger proteins (and optionally no more than 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, or 2 Zn finger proteins). In some embodiments, the Zn finger domain comprises at least three Zn finger proteins. In some embodiments, the Zn finger domain comprises four, five or six fingers. In some embodiments, the Zn finger domain comprises 8, 9, 10, 11 or 12 fingers. In some embodiments, a Zn finger domain comprising three Zn finger proteins recognizes a target DNA
sequence comprising 9 or 10 nucleotides. In some embodiments, a Zn finger domain comprising four Zn finger proteins recognizes a target DNA sequence comprising 12 to 14 nucleotides. In some embodiments, a Zn finger domain comprising six Zn finger proteins recognizes a target DNA sequence comprising 18 to 21 nucleotides.
In some embodiments, a Zn finger domain comprises a two-handed Zn finger protein. Two handed zinc finger proteins are those proteins in which two clusters of zinc finger proteins are separated by intervening amino acids so that the two zinc finger domains bind to two discontinuous target DNA
sequences. An example of a two-handed type of zinc finger binding protein is SIP1, where a cluster of four zinc finger proteins is located at the amino terminus of the protein and a cluster of three Zn finger proteins is located at the carboxyl terminus (see Remade, et al. (1999) EMBO
Journal 18(18):5073-5084).
Each cluster of zinc fingers in these proteins is able to bind to a unique target sequence and the spacing between the two target sequences can comprise many nucleotides.
Nucleic acid molecule In some embodiments, a DNA-targeting moiety is or comprises a DNA-binding domain from a nuclease.
For example, the recognition sequences of homing endonucleases and meganucleases such as I-SceI, I-CeuI, PI-PspI, PI-Sce, I-SceIV, I-CsmI, I-PanI, I-SceII, I-PpoI, I-SceIII, I-CreI, I-TevI, I-TevII and I-TevIII are known. See also U.S. Pat. Nos. 5,420,032; 6,833,252; Belfort, et al. (1997) Nucleic Acids Res.
25:3379-3388; Dujon, et al. (1989) Gene 82:115-118; Perler, et al. (1994) Nucleic Acids Res. 22:1125-1127; Jasin (1996) Trends Genet. 12:224-228; Gimble, et al. (1996) J. Mol.
Biol. 263:163-180; Argast, et al. (1998) J. Mol. Biol. 280:345-353 and the New England Biolabs catalogue. In addition, the DNA-binding specificity of homing endonucleases and meganucleases can be engineered to bind non-natural target sites. See, for example, Chevalier, et al. (2002) Molec. Cell 10:895-905; Epinat, et al. (2003) Nucleic Acids Res. 31:2952-2962; Ashworth, et al. (2006) Nature 441:656-659;
Paques, et al. (2007) Current Gene Therapy 7:49-66; U.S. Patent Publication No. 2007/0117128.
In some embodiments, a DNA-targeting moiety comprises or is nucleic acid. In some embodiments, a nucleic acid that may be included in a DNA-targeting moiety, may be or comprise DNA, RNA, and/or an artificial or synthetic nucleic acid or nucleic acid analog or mimic. For example, in some embodiments, a nucleic acid may be or include one or more of genomic DNA
(gDNA), complementary DNA (cDNA), a peptide nucleic acid (PNA), a peptide- oligonucleotide conjugate, a locked nucleic acid (LNA), a bridged nucleic acid (B NA), a polyamide, a triplex- forming oligonucleotide, an antisense oligonucleotide, tRNA, mRNA, rRNA, miRNA, gRNA, siRNA or other RNAi molecule (e.g., that targets a non-coding RNA as described herein and/or that targets an expression product of a particular gene associated with a targeted genomic complex as described herein), etc. In some embodiments, a nucleic acid may include one or more residues that is not a naturally occurring DNA or RNA residue, may include one or more linkages that is/are not phosphodiester bonds (e.g., that may be, for example, phosphorothioate bonds, etc.), and/or may include one or more modifications such as, for example, a 2'0 modification such as 2'-0MeP. A variety of nucleic acid structures useful in preparing synthetic nucleic acids is known in the art (see, for example, W02017/0628621 and W02014/012081) those skilled in the art will appreciate that these may be utilized in accordance with the present disclosure.
A nucleic acid suitable for use in an expression repressor, e.g., in the DNA-targeting moiety, may include, but is not limited to, DNA, RNA, modified oligonucleotides (e.g., chemical modifications, such as modifications that alter backbone linkages, sugar molecules, and/or nucleic acid bases), and artificial nucleic acids. In some embodiments, a nucleic acid includes, but is not limited to, genomic DNA, cDNA, peptide nucleic acids (PNA) or peptide oligonucleotide conjugates, locked nucleic acids (LNA), bridged nucleic acids (BNA), polyamides, triplex forming oligonucleotides, modified DNA, antisense DNA
oligonucleotides, tRNA, mRNA, rRNA, modified RNA, miRNA, gRNA, and siRNA or other RNA or DNA molecules.
In some embodiments, a DNA-targeting moiety comprises a nucleic acid with a length from about 15-200, 20-200, 30-200, 40-200, 50-200, 60-200, 70-200, 80-200, 90-200, 100-200, 110-200, 120-200, 130-200, 140-200, 150-200, 160-200, 170-200, 180-200, 190-200, 215-190, 20-190, 30-190, 40-190, 50-190, 60-190, 70-190, 80-190, 90-190, 100-190, 110-190, 120-190, 130-190, 140-190, 150-190, 160-190, 170-190, 180-190, 15-180, 20-180, 30-180, 40-180, 50-180, 60-180, 70-180, 80-180, 90-180, 100-180, 110-180, 120-180, 130-180, 140-180, 150-180, 160-180, 170-180, 15-170, 20-170, 30-170, 40-170, 50-170, 60-170, 70-170, 80-170, 90-170, 100-170, 110-170, 120-170, 130-170, 140-170, 150-170, 160-170, 15-160, 20-160, 30-160, 40-160, 50-160, 60-160, 70-160, 80-160, 90-160, 100-160, 110-160, 120-160, 130-160, 140-160, 150-160, 215-150, 20-150, 30-150, 40-150, 50-150, 60-150, 70-150, 80-150, 90-150, 100-150, 110-150, 120-150, 130-150, 140-150, 15-140, 20-140, 30-140, 40-140, 50-140, 60-140, 70-140, 80-140, 90-140, 100-140, 110-140, 120-140, 130-140, 15-130, 20-130, 30-130, 40-130, 50-130, 60-130, 70-130, 80-130, 90-130, 100-130, 110-130, 120-130, 215-120, 20-120, 30-120, 40-120, 50-120, 60-120, 70-120, 80-120, 90-120, 100-120, 110-120, 15-110, 20-110, 30-110, 40-110, 50-110, 60-110, 70-110, 80-110, 90-110, 100-110, 15-100, 20-100, 30-100, 40-100, 50-100, 60-100, 70-100, 80-100, 90-100, 15-90, 20-90, 30-90, 40-90, 50-90, 60-90, 70-90, 80-90, 15-80, 20-80, 30-80, 40-80, 50-80, 60-80, 70-80, 15-70, 20-70, 30-70, 40-70, 50-70, 60-70, 15-60, 20-60, 30-60, 40-60, 50-60, 15-50, 20-50, 30-50, 40-50, 15-40, 20-40, 30-40, 15-30, 20-30, or 15-20 nucleotides, or any range therebetween.
Repressor Domains Expression repressors of the present disclosure comprise one or more repressor domains. A
repressor domain has one or more functionality that, when used as part of an expressor repressor or an expression repression system described herein, decreases expression of a target gene in a cell.
In some embodiments, a repressor domain comprises a histone modifying functionality, e.g., a histone methyltransferase, histone demethylase, or histone deacetylase activity. In some embodiments, a histone methyltransferase functionality comprises H3K9 targeting methyltransferase activity. In some embodiments, a histone methyltransferase functionality comprises H3K56 targeting methyltransferase activity. In some embodiments, a histone methyltransferase functionality comprises H3K27 targeting methyltransferase activity. In some embodiments, a histone methyltransferase or demethylase functionality transfers one, two, or three methyl groups. In some embodiments, a histone demethylase functionality comprises H3K4 targeting demethylase activity. In some embodiments, a repressor domain is or comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, or a functional variant or fragment of any thereof, e.g., a SET domain of any thereof. In some embodiments, a repressor domain is or comprises a protein chosen from KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, or a functional variant or fragment of any thereof. In some embodiments, a repressor domain is or comprises a protein chosen from HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof.
In some embodiments, a repressor domain comprises an epigenetic modifying moiety. In some embodiments, a repressor domain comprises a DNA modifying functionality, e.g., a DNA
methyltransferase. In some embodiments, a repressor domain is or comprises a protein chosen from MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L or a functional variant or fragment of any thereof.
In some embodiments, a repressor domain comprises a transcription repressor.
In some embodiments the transcription repressor blocks recruitment of a factor that stimulates or promotes transcription, e.g., of the target gene. In some embodiments, the transcription repressor recruits a factor that inhibits transcription, e.g., of the target gene. In some embodiments, a repressor domain, e.g., transcription repressor, is or comprises a protein chosen from KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment of any thereof.
In some embodiments a repressor domain promotes epigenetic modification, e.g., directly, or indirectly. For example, a repressor domain can indirectly promote epigenetic modification by recruiting an endogenous protein that epigenetically modifies the chromatin. In some embodiments, a repressor domain can directly promote epigenetic modification by catalyzing epigenetic modification, wherein the repressor domain comprises enzymatic activity and directly places an epigenetic mark on the chromatin.
In some embodiments, a repressor domain comprises a protein having a functionality described herein. In some embodiments, a repressor domain is or comprises a protein selected from:
KRAB (e.g., as according to NP_056209.2 or the protein encoded by NM_015394.5, e.g., as according to SEQ ID NO: 61);
a SET domain (e.g., the SET domain of:
SETDB1 (e.g., as according to NP_001353347.1 or the protein encoded by NM_001366418.1);
EZH2 (e.g., as according to NP-004447.2 or the protein encoded by NM_004456.5, e.g., as according to SEQ ID NO: 65);
G9A (e.g., as according to NP_001350618.1 or the protein encoded by NM_001363689.1; e.g., as according to SEQ ID NO: 62); or SUV39H1 (e.g., as according to NP_003164.1 or the protein encoded by NM_003173.4));
histone demethylase LSD1 (e.g., as according to NP_055828.2 or the protein encoded by NM_015013.4, e.g., as according to SEQ ID NO: 64);
FOG1 (e.g., the N-terminal residues of FOG1) (e.g., as according to NP_722520.2 or the protein encoded by NM_153813.3, e.g., as according to SEQ ID NO: 66);
KAP1 (e.g., as according to NP_005753.1 or the protein encoded by NM_005762.3); or HDAC8 (e.g., as according to NP_001159890 or the protein encoded by NM_001166418, e.g., as according to SEQ ID NO: 63);
a functional fragment or variant of any thereof, or a polypeptide with a sequence that has at least 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identity to any of the above-referenced sequences. In some embodiments, a repressor domain is or comprises a protein selected from:
DNMT3A (e.g., human DNMT3A) (e.g., as according to NP_072046.2 or the protein encoded by NM_022552.4, e.g., as according to SEQ ID NO: 58);
DNMT3B (e.g., as according to or A0X21819.1 .. or the protein encoded by NM_006892.4 or I(X447429);
DNMT3L (e.g., as according to NP_787063.1 or the protein encoded by NM_175867.3);
DNMT3A/3L complex (e.g., as according to SEQ ID NO: 59 or 60), bacterial MQ1 (e.g., as according to CAA35058.1 or P15840.3, e.g., as according to SEQ ID NO: 57 or 90);
a functional fragment of any thereof, or a polypeptide with a sequence that has at least 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identity to any of the above-referenced sequences.
.. In some embodiments, a repressor domain is or comprises a polypeptide. In some embodiments, a repressor domain has enzymatic activity.
In some embodiments, a DNA-binding domain comprises a helix-hairpin-helix (HhH) motif.
DNA-binding proteins with a HhH structural motif may be involved in non-sequence-specific DNA
.. binding that occurs via the formation of hydrogen bonds between protein backbone nitrogens and DNA
phosphate groups.
In some embodiments, a DNA-binding domain comprises a helix-loop-helix (HLH) motif. DNA-binding proteins with an HLH structural motif are transcriptional regulatory proteins and are principally related to a wide array of developmental processes. An HLH structural motif is longer, in terms of residues, than HTH or HhH motifs. Many of these proteins interact to form homo-and hetero-dimers. A
structural motif is composed of two long helix regions, with an N-terminal helix binding to DNA, while a complex region allows the protein to dimerize.
In some embodiments, a DNA-binding domain comprises a leucine zipper motif. In some transcription factors, a dimer binding site with DNA forms a leucine zipper.
This motif includes two amphipathic helices, one from each subunit, interacting with each other resulting in a left-handed coiled-coil super secondary structure. A leucine zipper is an interdigitation of regularly spaced leucine residues in one helix with leucines from an adjacent helix. Mostly, helices involved in leucine zippers exhibit a heptad sequence (abcdefg) with residues a and d being hydrophobic and other residues being hydrophilic.
Leucine zipper motifs can mediate either homo- or heterodimer formation.
In some embodiments, a DNA-binding domain comprises a Zn finger domain, where a Zn++ ion is coordinated by 2 Cys and 2 His residues. Such a transcription factor includes a trimer with the stoichiometry 1313 'a. An apparent effect of Zn++ coordination is stabilization of a small complex structure instead of hydrophobic core residues. Each Zn-finger interacts in a conformationally identical manner with successive triple base pair segments in the major groove of the double helix. Protein-DNA
interaction is determined by two factors: (i) H-bonding interaction between a-helix and DNA segment, mostly between Arg residues and Guanine bases. (ii) H-bonding interaction with DNA phosphate backbone, mostly with Arg and His. An alternative Zn-finger motif chelates Zn++ with 6 Cys.
An exemplary repressor domain may include, but is not limited to: ubiquitin, bicyclic peptides as ubiquitin ligase inhibitors, transcription factors, DNA and protein modification enzymes such as topoisomerases, topoisomerase inhibitors such as topotecan, DNA
methyltransferases such as the DNMT
family (e.g., DNMT3A, DNMT3B, DNMT3L), protein methyltransferases (e.g., viral lysine methyltransferase (vSET), protein-lysine N-methyltransferase (SMYD2), deaminases (e.g., APOBEC, UG1), histone methyltransferases such as enhancer of zeste homolog 2 (EZH2), PRMT1, histone-lysine-N-methyltransferase (Setdbl), histone methyltransferase (SET2), euchromatic histone-lysine N-methyltransferase 2 (G9A), histone-lysine N-methyltransferase (SUV39H1), and G9a), histone deacetylase (e.g., HDAC1, HDAC2, HDAC3, HDAC8), enzymes with a role in DNA
demethylation (e.g., the TET family enzymes catalyze oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and higher oxidative derivatives), protein demethylases such as KDM1A and lysine-specific histone demethylase 1 (LSD1), transcription repressors (e.g., KRAB, FOG1), helicases such as DHX9, deacetylases (e.g., sirtuin 1, 2, 3, 4, 5, 6, or 7), kinases, phosphatases, DNA-intercalating agents such as ethidium bromide, SYBR
green, and proflavine, efflux pump inhibitors such as peptidomimetics like phenylalanine arginyl 13-naphthylamide or quinoline derivatives, nuclear receptor activators and inhibitors, proteasome inhibitors, competitive inhibitors for enzymes such as those involved in lysosomal storage diseases, protein synthesis inhibitors, nucleases (e.g., Cpfl, Cas9, zinc finger nuclease), fusions of one or more thereof (e.g., dCas9-DNMT, dCas9-APOBEC, dCas9-UG1), and specific domains from proteins, such as a KRAB domain.
In some embodiments, a candidate domain may be determined to be suitable for use as a repressor domain by methods known to those of skill in the art. For example, a candidate repressor domain may be tested by assaying whether, when the candidate repressor domain is present in the nucleus of a cell and appropriately localized (e.g., to a target gene or transcription control element operably linked to said target gene, e.g., via a DNA-targeting moiety), the candidate repressor domain decreases expression of the target gene in the cell, e.g., decreases the level of RNA
transcript encoded by the target gene (e.g., as measured by RNASeq or Northern blot) or decreases the level of protein encoded by the target gene (e.g., as measured by ELISA).
In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each member of the plurality of expression repressors comprises a repressor domain, wherein each repressor domain does not detectably bind, e.g., does not bind, to another repressor domain.
In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor domain, wherein the first repressor domain does not detectably bind, e.g., does not bind, to the second repressor domain. In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor domain, wherein the first repressor domain does not detectably bind, e.g., does not bind, to another first repressor domain, and the second repressor domain does not detectably bind, e.g., does not bind, to another second repressor domain. In some embodiments, a repressor domain for use in the compositions and methods described herein is functional in a monomeric, e.g., non-dimeric, state.
In some embodiments, a repressor domain is or comprises an epigenetic modifying moiety, e.g., that modulates the two-dimensional structure of chromatin (i.e., that modulate structure of chromatin in a way that would alter its two-dimensional representation).
Epigenetic modifying moieties useful in methods and compositions of the present disclosure include agents that affect epigenetic markers, e.g., DNA methylation, histone methylation, histone acetylation, histone sumoylation, histone phosphorylation, and RNA-associated silencing. Exemplary epigenetic enzymes that can be targeted to a genomic sequence element as described herein include DNA
methylases (e.g., DNMT3a, DNMT3b, DNMTL), DNA demethylation (e.g., the TET
family), histone methyltransferases, histone deacetylase (e.g., HDAC1, HDAC2, HDAC3), sirtuin 1, 2, 3, 4, 5, 6, or 7, lysine-specific histone demethylase 1 (LSD1), histone-lysine-N-methyltransferase (Setdbl), euchromatic histone-lysine N-methyltransferase 2 (G9a), histone-lysine N-methyltransferase (SUV39H1), enhancer of zeste homolog 2 (EZH2), viral lysine methyltransferase (vSET), histone methyltransferase (SET2), and protein-lysine N-methyltransferase (SMYD2). Examples of such epigenetic modifying agents are described, e.g., in de Groote et al. Nuc. Acids Res. (2012):1-18.
In some embodiments, an expression repressor, e.g., comprising an epigenetic modifying moiety, useful herein comprises or is a construct described in Koferle et al. Genome Medicine 7.59 (2015):1-3incorporated herein by reference. For example, in some embodiments, an expression repressor comprises or is a construct found in Table 1 of Koferle et al., e.g., histone deacetylase, histone methyltransferase, DNA demethylation, or H3K4 and/or H3K9 histone demethylase described in Table 1 (e.g., dCas9-p300, TALE-TET1, ZF-DNMT3A, or TALE-LSD1).
Additional Moieties An expression repressor may further comprise one or more additional moieties (e.g., in addition to one or more DNA-targeting moieties and one or more repressor domains). In some embodiments, an additional moiety is selected from a tagging or monitoring moiety, a cleavable moiety (e.g., a cleavable moiety positioned between a DNA-targeting moiety and a repressor domain or at the N- or C-terminal end of a polypeptide), a small molecule, a membrane translocating polypeptide, or a pharmacoagent moiety.
Exemplary Expression Repressors The following exemplary expression repressors are presented for illustration purposes only and are not intended to be limiting.
In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. aureus dCas9, and a repressor domain comprising MQ1, e.g., bacterial MQ1. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NO: 91 (e.g., a plasmid encoding the expression repressor), SEQ ID NO: 22 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor) and/or SEQ ID NO: 92 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 22, 91 or 92 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto. In some embodiments, the DNA-targeting moiety is encoded by the nucleic acid sequence of SEQ ID NO: 45 or 89 and/or the repressor domain is encoded by the nucleic acid sequence of SEQ ID NO: 47.
dCas9-MQ1 (MR-28125) mRNA sequence:
AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAAGCGGCCCGCCG
CCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGCCCGGGACAGCAAGGTGGAGAACA
AGACCAAGAAGCTGCGGGTGTTCGAGGCCTTCGCCGGCATCGGCGCCCAGCGGAAGGCCCT
GGAGAAGGTGCGGAAGGACGAGTACGAGATCGTGGGCCTGGCCGAGTGGTACGTGCCCGCC
ATCGTGATGTACCAGGCCATCCACAACAACTTCCACACCAAGCTGGAGTACAAGAGCGTGA
GCCGGGAGGAGATGATCGACTACCTGGAGAACAAGACCCTGAGCTGGAACAGCAAGAACCC
CGTGAGCAACGGCTACTGGAAGCGGAAGAAGGACGACGAGCTGAAGATCATCTACAACGCC
ATCAAGCTGAGCGAGAAGGAGGGCAACATCTTCGACATCCGGGACCTGTACAAGCGGACCC
TGAAGAACATCGACCTGCTGACCTACAGCTTCCCCTGCCAGGACCTGAGCCAGCAGGGCATC
CAGAAGGGCATGAAGCGGGGCAGCGGCACCCGGAGCGGCCTGCTGTGGGAGATCGAGCGG
GCCCTGGACAGCACCGAGAAGAACGACCTGCCCAAGTACCTGCTGATGGAGAACGTGGGCG
CCCTGCTGCACAAGAAGAACGAGGAGGAGCTGAACCAGTGGAAGCAGAAGCTGGAGAGCC
TGGGCTACCAGAACAGCATCGAGGTGCTGAACGCCGCCGACTTCGGCAGCAGCCAGGCCCG
GCGGCGGGTGTTCATGATCAGCACCCTGAACGAGTTCGTGGAGCTGCCCAAGGGCGACAAG
AAGCCCAAGAGCATCAAGAAGGTGCTGAACAAGATCGTGAGCGAGAAGGACATCCTGAAC
AACCTGCTGAAGTACAACCTGACCGAGTTCAAGAAAACCAAGAGCAACATCAACAAGGCCA
GCCTGATCGGCTACAGCAAGTTCAACAGCGAGGGCTACGTGTACGACCCCGAGTTCACCGG
CCCCACCCTGACCGCCAGCGGCGCCAACAGCCGGATCAAGATCAAGGACGGCAGCAACATC
CGGAAGATGAACAGCGACGAGACCTTCCTGTACATCGGCTTCGACAGCCAGGACGGCAAGC
GGGTGAACGAGATCGAGTTCCTGACCGAGAACCAGAAGATCTTCGTGTGCGGCAACAGCAT
CAGCGTGGAGGTGCTGGAGGCCATCATCGACAAGATCGGCGGCCCCAGCAGCGGCGGCAAG
CGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTAC
GACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTC
TGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTA
GGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 22) Sa-dCas9-MQ1 (PL-27695) Plasmid DNA sequence:
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACA
GCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTG
GCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCAT
ATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGC
CATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAG
CTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGT
CACGACGTTGTAAAACGACGGCCAGTGAATTGACGCGTATTGGGATGGTACCTAATACGACT
CACTATAAGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCC
CCCAAGAAGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGCCAAGCGGAACTACA
TCCTGGGCCTGGCCATCGGCATCACCAGCGTGGGCTACGGCATCATCGACTACGAGACCCGG
GACGTGATCGACGCCGGCGTGCGGCTGTTCAAGGAGGCCAACGTGGAGAACAACGAGGGCC
GGCGGAGCAAGCGGGGCGCCCGGCGGCTGAAGCGGCGGCGGCGGCACCGGATCCAGCGGG
TGAAGAAGCTGCTGTTCGACTACAACCTGCTGACCGACCACAGCGAGCTGAGCGGCATCAA
CCCCTACGAGGCCCGGGTGAAGGGCCTGAGCCAGAAGCTGAGCGAGGAGGAGTTCAGCGCC
GCCCTGCTGCACCTGGCCAAGCGGCGGGGCGTGCACAACGTGAACGAGGTGGAGGAGGACA
CCGGCAACGAGCTGAGCACCAAGGAGCAGATCAGCCGGAACAGCAAGGCCCTGGAGGAGA
AGTACGTGGCCGAGCTGCAGCTGGAGCGGCTGAAGAAGGACGGCGAGGTGCGGGGCAGCA
TCAACCGGTTCAAGACCAGCGACTACGTGAAGGAGGCCAAGCAGCTGCTGAAGGTGCAGAA
GGCCTACCACCAGCTGGACCAGAGCTTCATCGACACCTACATCGACCTGCTGGAGACCCGGC
GGACCTACTACGAGGGCCCCGGCGAGGGCAGCCCCTTCGGCTGGAAGGACATCAAGGAGTG
GTACGAGATGCTGATGGGCCACTGCACCTACTTCCCCGAGGAGCTGCGGAGCGTGAAGTAC
GCCTACAACGCCGACCTGTACAACGCCCTGAACGACCTGAACAACCTGGTGATCACCCGGG
ACGAGAACGAGAAGCTGGAGTACTACGAGAAGTTCCAGATCATCGAGAACGTGTTCAAGCA
GAAGAAGAAGCCCACCCTGAAGCAGATCGCCAAGGAGATCCTGGTGAACGAGGAGGACAT
CAAGGGCTACCGGGTGACCAGCACCGGCAAGCCCGAGTTCACCAACCTGAAGGTGTACCAC
GACATCAAGGACATCACCGCCCGGAAGGAGATCATCGAGAACGCCGAGCTGCTGGACCAGA
TCGCCAAGATCCTGACCATCTACCAGAGCAGCGAGGACATCCAGGAGGAGCTGACCAACCT
GAACAGCGAGCTGACCCAGGAGGAGATCGAGCAGATCAGCAACCTGAAGGGCTACACCGG
CACCCACAACCTGAGCCTGAAGGCCATCAACCTGATCCTGGACGAGCTGTGGCACACCAAC
GACAACCAGATCGCCATCTTCAACCGGCTGAAGCTGGTGCCCAAGAAGGTGGACCTGAGCC
AGCAGAAGGAGATCCCCACCACCCTGGTGGACGACTTCATCCTGAGCCCCGTGGTGAAGCG
GAGCTTCATCCAGAGCATCAAGGTGATCAACGCCATCATCAAGAAGTACGGCCTGCCCAAC
GACATCATCATCGAGCTGGCCCGGGAGAAGAACAGCAAGGACGCCCAGAAGATGATCAACG
AGATGCAGAAGCGGAACCGGCAGACCAACGAGCGGATCGAGGAGATCATCCGGACCACCG
GCAAGGAGAACGCCAAGTACCTGATCGAGAAGATCAAGCTGCACGACATGCAGGAGGGCA
AGTGCCTGTACAGCCTGGAGGCCATCCCCCTGGAGGACCTGCTGAACAACCCCTTCAACTAC
GAGGTGGACGCCATCATCCCCCGGAGCGTGAGCTTCGACAACAGCTTCAACAACAAGGTGC
TGGTGAAGCAGGAGGAGAACAGCAAGAAGGGCAACCGGACCCCCTTCCAGTACCTGAGCAG
CAGCGACAGCAAGATCAGCTACGAGACCTTCAAGAAGCACATCCTGAACCTGGCCAAGGGC
AAGGGCCGGATCAGCAAGACCAAGAAGGAGTACCTGCTGGAGGAGCGGGACATCAACCGG
TTCAGCGTGCAGAAGGACTTCATCAACCGGAACCTGGTGGACACCCGGTACGCCACCCGGG
GCCTGATGAACCTGCTGCGGAGCTACTTCCGGGTGAACAACCTGGACGTGAAGGTGaaatccAT
CAACGGCGGCTTCACCAGCTTCCTGCGGCGGAAGTGGAAGTTCAAGAAGGAGCGGAACAAG
GGCTACAAGCACCACGCCGAGGACGCCCTGATCATCGCCAACGCCGACTTCATCTTCAAGGA
GTGGAAGAAGCTGGACAAGGCCAAGAAGGTGATGGAGAACCAGATGTTCGAGGAGAAGCA
GGCCGAGAGCATGCCCGAGATCGAGACCGAGCAGGAGTACAAGGAGATCTTCATCACCCCC
CACCAGATCAAGCACATCAAGGACTTCAAGGACTACAAGTACAGCCACCGGGTGGACAAGA
AGCCCAACCGGAAGCTGATCAACGACACCCTGTACAGCACCCGGAAGGACGACAAGGGCAA
CACCCTGATCGTGAACAACCTGAACGGCCTGTACGACAAGGACAACGACAAGCTGAAGAAG
CTGATCAACAAGAGCCCCGAGAAGCTGCTGATGTACCACCACGACCCCCAGACCTACCAGA
AGCTGAAGCTGATCATGGAGCAGTACGGCGACGAGAAGAACCCCCTGTACAAGTACTACGA
GGAGACCGGCAACTACCTGACCAAGTACAGCAAGAAGGACAACGGCCCCGTGATCAAGAA
GATCAAGTACTACGGCAACAAGCTGAACGCCCACCTGGACATCACCGACGACTACCCCAAC
AGCCGGAACAAGGTGGTGAAGCTGAGCCTGAAGCCCTACCGGTTCGACGTGTACCTGGACA
ACGGCGTGTACAAGTTCGTGACCGTGAAGAACCTGGACGTGATCAAGAAGGAGAACTACTA
CGAGGTGAACAGCAAGTGCTACGAGGAGGCCAAGAAGCTGAAGAAGATCAGCAACCAGGC
CGAGTTCATCGCCAGCTTCTACAAGAACGACCTGATCAAGATCAACGGCGAGCTGTACCGG
GTGATCGGCGTGAACAACGACCTGCTGAACCGGATCGAGGTGAACATGATCGACATCACCT
ACCGGGAGTACCTGGAGAACATGAACGACAAGCGGCCCCCCCACATCATCAAGACCATCGC
CAGCAAGACCCAGAGCATCAAGAAGTACAGCACCGACATCCTGGGCAACCTGTACGAGGTG
aaatccAAGAAGCACCCCCAGATCATCAAGAAGGGCAAGCGGCCCGCCGCCACCAAGAAGGCC
GGCCAGGCCAAGAAGAAGAAGGCCCGGGACAGCAAGGTGGAGAACAAGACCAAGAAGCTG
CGGGTGTTCGAGGCCTTCGCCGGCATCGGCGCCCAGCGGAAGGCCCTGGAGAAGGTGCGGA
AGGACGAGTACGAGATCGTGGGCCTGGCCGAGTGGTACGTGCCCGCCATCGTGATGTACCA
GGCCATCCACAACAACTTCCACACCAAGCTGGAGTACAAGAGCGTGAGCCGGGAGGAGATG
ATCGACTACCTGGAGAACAAGACCCTGAGCTGGAACAGCAAGAACCCCGTGAGCAACGGCT
ACTGGAAGCGGAAGAAGGACGACGAGCTGAAGATCATCTACAACGCCATCAAGCTGAGCGA
GAAGGAGGGCAACATCTTCGACATCCGGGACCTGTACAAGCGGACCCTGAAGAACATCGAC
CTGCTGACCTACAGCTTCCCCTGCCAGGACCTGAGCCAGCAGGGCATCCAGAAGGGCATGA
AGCGGGGCAGCGGCACCCGGAGCGGCCTGCTGTGGGAGATCGAGCGGGCCCTGGACAGCAC
CGAGAAGAACGACCTGCCCAAGTACCTGCTGATGGAGAACGTGGGCGCCCTGCTGCACAAG
AAGAACGAGGAGGAGCTGAACCAGTGGAAGCAGAAGCTGGAGAGCCTGGGCTACCAGAAC
AGCATCGAGGTGCTGAACGCCGCCGACTTCGGCAGCAGCCAGGCCCGGCGGCGGGTGTTCA
TGATCAGCACCCTGAACGAGTTCGTGGAGCTGCCCAAGGGCGACAAGAAGCCCAAGAGCAT
CAAGAAGGTGCTGAACAAGATCGTGAGCGAGAAGGACATCCTGAACAACCTGCTGAAGTAC
AACCTGACCGAGTTCAAGAAaACCAAGAGCAACATCAACAAGGCCAGCCTGATCGGCTACA
GCAAGTTCAACAGCGAGGGCTACGTGTACGACCCCGAGTTCACCGGCCCCACCCTGACCGCC
AGCGGCGCCAACAGCCGGATCAAGATCAAGGACGGCAGCAACATCCGGAAGATGAACAGC
GACGAGACCTTCCTGTACATCGGCTTCGACAGCCAGGACGGCAAGCGGGTGAACGAGATCG
AGTTCCTGACCGAGAACCAGAAGATCTTCGTGTGCGGCAACAGCATCAGCGTGGAGGTGCT
GGAGGCCATCATCGACAAGATCGGCGGCCCCAGCAGCGGCGGCAAGCGGCCCGCCGCCACC
AAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTAC
GCCTGAGcggccgcttaattaagctgccttctgcggggcttgccttctggccatgcccttcttctctcccttgcacctg tacctcttggtctttgaataaa gcctgagtaggaagtctagaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa aaaaaaaaaaaaaaaaa aaattgtcttcttcatcgcctgcagATCCCAATGGCGCGCCGAGCTTGGCTCGAGCATGGTCATAGCTGTTTC
CTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT
AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC
TTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA
GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTT
CGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGA
CGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTA
TCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC
CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGG
TGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGT
TACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTG
ATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCAT
GAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAA
TCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTAGAAAAACTCATCGAGCATCAAATGA
AACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT
GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGA
TTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAA
GTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCT
TTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAA
ACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGAC
AATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATT
TTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCAGGGATCGCAGTGGT
GAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAAT
TCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCA
TGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGA
TTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAA
TCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTA
TTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAA
ATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAAC
CATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC (SEQ ID
NO: 91) Sa-dCas9-MQ1 (MR-28126) Expressed mRNA sequence:
AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGCCAAGCGGAACTACATCCTGGG
CCTGGCCATCGGCATCACCAGCGTGGGCTACGGCATCATCGACTACGAGACCCGGGACGTG
ATCGACGCCGGCGTGCGGCTGTTCAAGGAGGCCAACGTGGAGAACAACGAGGGCCGGCGGA
GCAAGCGGGGCGCCCGGCGGCTGAAGCGGCGGCGGCGGCACCGGATCCAGCGGGTGAAGA
AGCTGCTGTTCGACTACAACCTGCTGACCGACCACAGCGAGCTGAGCGGCATCAACCCCTAC
GAGGCCCGGGTGAAGGGCCTGAGCCAGAAGCTGAGCGAGGAGGAGTTCAGCGCCGCCCTGC
TGCACCTGGCCAAGCGGCGGGGCGTGCACAACGTGAACGAGGTGGAGGAGGACACCGGCA
ACGAGCTGAGCACCAAGGAGCAGATCAGCCGGAACAGCAAGGCCCTGGAGGAGAAGTACG
TGGCCGAGCTGCAGCTGGAGCGGCTGAAGAAGGACGGCGAGGTGCGGGGCAGCATCAACC
GGTTCAAGACCAGCGACTACGTGAAGGAGGCCAAGCAGCTGCTGAAGGTGCAGAAGGCCTA
CCACCAGCTGGACCAGAGCTTCATCGACACCTACATCGACCTGCTGGAGACCCGGCGGACCT
ACTACGAGGGCCCCGGCGAGGGCAGCCCCTTCGGCTGGAAGGACATCAAGGAGTGGTACGA
GATGCTGATGGGCCACTGCACCTACTTCCCCGAGGAGCTGCGGAGCGTGAAGTACGCCTACA
ACGCCGACCTGTACAACGCCCTGAACGACCTGAACAACCTGGTGATCACCCGGGACGAGAA
CGAGAAGCTGGAGTACTACGAGAAGTTCCAGATCATCGAGAACGTGTTCAAGCAGAAGAAG
AAGCCCACCCTGAAGCAGATCGCCAAGGAGATCCTGGTGAACGAGGAGGACATCAAGGGCT
ACCGGGTGACCAGCACCGGCAAGCCCGAGTTCACCAACCTGAAGGTGTACCACGACATCAA
GGACATCACCGCCCGGAAGGAGATCATCGAGAACGCCGAGCTGCTGGACCAGATCGCCAAG
ATCCTGACCATCTACCAGAGCAGCGAGGACATCCAGGAGGAGCTGACCAACCTGAACAGCG
AGCTGACCCAGGAGGAGATCGAGCAGATCAGCAACCTGAAGGGCTACACCGGCACCCACAA
CCTGAGCCTGAAGGCCATCAACCTGATCCTGGACGAGCTGTGGCACACCAACGACAACCAG
ATCGCCATCTTCAACCGGCTGAAGCTGGTGCCCAAGAAGGTGGACCTGAGCCAGCAGAAGG
AGATCCCCACCACCCTGGTGGACGACTTCATCCTGAGCCCCGTGGTGAAGCGGAGCTTCATC
CAGAGCATCAAGGTGATCAACGCCATCATCAAGAAGTACGGCCTGCCCAACGACATCATCA
TCGAGCTGGCCCGGGAGAAGAACAGCAAGGACGCCCAGAAGATGATCAACGAGATGCAGA
AGCGGAACCGGCAGACCAACGAGCGGATCGAGGAGATCATCCGGACCACCGGCAAGGAGA
ACGCCAAGTACCTGATCGAGAAGATCAAGCTGCACGACATGCAGGAGGGCAAGTGCCTGTA
CAGCCTGGAGGCCATCCCCCTGGAGGACCTGCTGAACAACCCCTTCAACTACGAGGTGGAC
GCCATCATCCCCCGGAGCGTGAGCTTCGACAACAGCTTCAACAACAAGGTGCTGGTGAAGC
AGGAGGAGAACAGCAAGAAGGGCAACCGGACCCCCTTCCAGTACCTGAGCAGCAGCGACA
GCAAGATCAGCTACGAGACCTTCAAGAAGCACATCCTGAACCTGGCCAAGGGCAAGGGCCG
GATCAGCAAGACCAAGAAGGAGTACCTGCTGGAGGAGCGGGACATCAACCGGTTCAGCGTG
CAGAAGGACTTCATCAACCGGAACCTGGTGGACACCCGGTACGCCACCCGGGGCCTGATGA
ACCTGCTGCGGAGCTACTTCCGGGTGAACAACCTGGACGTGAAGGTGaaatccATCAACGGCGG
CTTCACCAGCTTCCTGCGGCGGAAGTGGAAGTTCAAGAAGGAGCGGAACAAGGGCTACAAG
CACCACGCCGAGGACGCCCTGATCATCGCCAACGCCGACTTCATCTTCAAGGAGTGGAAGA
AGCTGGACAAGGCCAAGAAGGTGATGGAGAACCAGATGTTCGAGGAGAAGCAGGCCGAGA
GCATGCCCGAGATCGAGACCGAGCAGGAGTACAAGGAGATCTTCATCACCCCCCACCAGAT
CAAGCACATCAAGGACTTCAAGGACTACAAGTACAGCCACCGGGTGGACAAGAAGCCCAAC
CGGAAGCTGATCAACGACACCCTGTACAGCACCCGGAAGGACGACAAGGGCAACACCCTGA
TCGTGAACAACCTGAACGGCCTGTACGACAAGGACAACGACAAGCTGAAGAAGCTGATCAA
CAAGAGCCCCGAGAAGCTGCTGATGTACCACCACGACCCCCAGACCTACCAGAAGCTGAAG
CTGATCATGGAGCAGTACGGCGACGAGAAGAACCCCCTGTACAAGTACTACGAGGAGACCG
GCAACTACCTGACCAAGTACAGCAAGAAGGACAACGGCCCCGTGATCAAGAAGATCAAGTA
CTACGGCAACAAGCTGAACGCCCACCTGGACATCACCGACGACTACCCCAACAGCCGGAAC
AAGGTGGTGAAGCTGAGCCTGAAGCCCTACCGGTTCGACGTGTACCTGGACAACGGCGTGT
ACAAGTTCGTGACCGTGAAGAACCTGGACGTGATCAAGAAGGAGAACTACTACGAGGTGAA
CAGCAAGTGCTACGAGGAGGCCAAGAAGCTGAAGAAGATCAGCAACCAGGCCGAGTTCATC
GCCAGCTTCTACAAGAACGACCTGATCAAGATCAACGGCGAGCTGTACCGGGTGATCGGCG
TGAACAACGACCTGCTGAACCGGATCGAGGTGAACATGATCGACATCACCTACCGGGAGTA
CCTGGAGAACATGAACGACAAGCGGCCCCCCCACATCATCAAGACCATCGCCAGCAAGACC
CAGAGCATCAAGAAGTACAGCACCGACATCCTGGGCAACCTGTACGAGGTGaaatccAAGAAG
CACCCCCAGATCATCAAGAAGGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCA
AGAAGAAGAAGGCCCGGGACAGCAAGGTGGAGAACAAGACCAAGAAGCTGCGGGTGTTCG
AGGCCTTCGCCGGCATCGGCGCCCAGCGGAAGGCCCTGGAGAAGGTGCGGAAGGACGAGTA
CGAGATCGTGGGCCTGGCCGAGTGGTACGTGCCCGCCATCGTGATGTACCAGGCCATCCACA
ACAACTTCCACACCAAGCTGGAGTACAAGAGCGTGAGCCGGGAGGAGATGATCGACTACCT
GGAGAACAAGACCCTGAGCTGGAACAGCAAGAACCCCGTGAGCAACGGCTACTGGAAGCG
GAAGAAGGACGACGAGCTGAAGATCATCTACAACGCCATCAAGCTGAGCGAGAAGGAGGG
CAACATCTTCGACATCCGGGACCTGTACAAGCGGACCCTGAAGAACATCGACCTGCTGACCT
ACAGCTTCCCCTGCCAGGACCTGAGCCAGCAGGGCATCCAGAAGGGCATGAAGCGGGGCAG
CGGCACCCGGAGCGGCCTGCTGTGGGAGATCGAGCGGGCCCTGGACAGCACCGAGAAGAAC
GACCTGCCCAAGTACCTGCTGATGGAGAACGTGGGCGCCCTGCTGCACAAGAAGAACGAGG
AGGAGCTGAACCAGTGGAAGCAGAAGCTGGAGAGCCTGGGCTACCAGAACAGCATCGAGG
TGCTGAACGCCGCCGACTTCGGCAGCAGCCAGGCCCGGCGGCGGGTGTTCATGATCAGCAC
CCTGAACGAGTTCGTGGAGCTGCCCAAGGGCGACAAGAAGCCCAAGAGCATCAAGAAGGTG
CTGAACAAGATCGTGAGCGAGAAGGACATCCTGAACAACCTGCTGAAGTACAACCTGACCG
AGTTCAAGAAaACCAAGAGCAACATCAACAAGGCCAGCCTGATCGGCTACAGCAAGTTCAA
CAGCGAGGGCTACGTGTACGACCCCGAGTTCACCGGCCCCACCCTGACCGCCAGCGGCGCC
AACAGCCGGATCAAGATCAAGGACGGCAGCAACATCCGGAAGATGAACAGCGACGAGACC
TTCCTGTACATCGGCTTCGACAGCCAGGACGGCAAGCGGGTGAACGAGATCGAGTTCCTGAC
CGAGAACCAGAAGATCTTCGTGTGCGGCAACAGCATCAGCGTGGAGGTGCTGGAGGCCATC
ATCGACAAGATCGGCGGCCCCAGCAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCG
GCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGcggccg cttaattaagctgccttctgcggggcttgccttctggccatgcccttcttctctcccttgcacctgtacctcttggtct ttgaataaagcctgagtaggaagtcta gaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa aa (SEQ ID NO:
92) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 93, 33, 67, or 68. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 93, 33, 67, 68, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
Sa-dCas9-MQ1 Protein sequence:
MAPKKKRKVGIHGVPAAAKRNYILGLAIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRR
SKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLA
KRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYV
KEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYF
PEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKEILV
NEEDIKGYRVT STGKPEFTNLKVYHDIKDITARKEIIENAELLD QIAKILTIY QS S EDIQEELTNLNS
ELT QEEIEQIS NLKGYTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLS QQKEIPTTL
VDDFILSPVVKRSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEII
RTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDAIIPRSVSFDNSFNNKVLV
.. KQEENS KKGNRTPFQYLSS SD S KISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFI
NRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKS INGGFT SFLRRKWKFKKERNKGYKHHAED
ALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQIKHIKDFKDYK
YSHRVDKKPNRKLINDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLKKLINKSPEKLLMYHHD
PQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYS KKDNGPVIKKIKYYGNKLNAHLDITDD
YPNS RNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNS KCYEEAKKLKKIS NQ
AEFIAS FYKNDLIKINGELYRVIGVNNDLLNRIEVNMIDITYREYLENMND KRPPHIIKTIAS KT QSI
KKYSTDILGNLYEVKSKKHPQIIKKGKRPAATKKAGQAKKKKARDSKVENKTKKLRVFEAFAGI
GAQRKALEKVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEYKSVSREEMIDYLENKTLSW
NS KNPV SNGYWKRKKDDELKIIYNAIKLS EKEGNIFDIRDLYKRTLKNIDLLTYSFPC QDLS QQGI
QKGMKRGSGTRSGLLWEIERALDSTEKNDLPKYLLMENVGALLHKKNEEELNQWKQKLESLG
YQNSIEVLNAADFGS S QARRRVFMISTLNEFVELPKGDKKPKSIKKVLNKIVSEKDILNNLLKYNL
TEFKKTKSNINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRIKIKDGSNIRKMNSDETFLYIG
FDS QDGKRVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGGPSSGGKRPAATKKAGQAKKKKGSY
PYDVPDYA (SEQ ID NO: 93) dCas9-MQ1 amino acid sequence MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGD KRPAATKKAGQAKKKKARD S KVENKTKKLRVFEAFAGIGAQRKALE
KVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEYKSV SREEMIDYLENKTLSWNS KNPVS N
GYWKRKKDDELKIIYNAIKLSEKEGNIFDIRDLYKRTLKNIDLLTYSFPCQDLSQQGIQKGMKRG
SGTRSGLLWEIERALDSTEKNDLPKYLLMENVGALLHKKNEEELNQWKQKLESLGYQNSIEVLN
AADFGS SQARRRVFMISTLNEFVELPKGDKKPKSIKKVLNKIVSEKDILNNLLKYNLTEFKKTKS
NINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRIKIKDGSNIRKMNSDETFLYIGFDS QDGK
RVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGGPSSGGKRPAATKKAGQAKKKKGSYPYDVPDY
A (SEQ ID NO: 33) Sa-dCas9-MQ1 without HA tag MAPKKKRKVGIHGVPAAAKRNYILGLAIGITS VGYGIIDYETRDVIDAGVRLFKEANVENNEGRR
S KRGARRLKRRRRHRIQRV KKLLFDYNLLTDHSELS GINPYEARVKGLS QKLSEEEFSAALLHLA
KRRGVHNVNEVEEDTGNELSTKEQISRNS KALEEKYVAELQLERLKKDGEVRGSINRFKTSDYV
KEAKQLLKV QKAYHQLD QSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYF
PEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKEILV
NEEDIKGYRVT STGKPEFTNLKVYHDIKDITARKEIIENAELLD QIAKILTIY QS S EDIQEELTNLNS
ELT QEEIEQIS NLKGYTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLS QQKEIPTTL
VDDFILSPVVKRSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEII
RTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDAIIPRSVSFDNSFNNKVLV
KQEENS KKGNRTPFQYLSS SD S KISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFI
NRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKS INGGFT SFLRRKWKFKKERNKGYKHHAED
ALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQIKHIKDFKDYK
YSHRVDKKPNRKLINDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLKKLINKSPEKLLMYHHD
PQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYS KKDNGPVIKKIKYYGNKLNAHLDITDD
YPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAKKLKKISNQ
AEFIAS FYKNDLIKINGELYRVIGVNNDLLNRIEVNMIDITYREYLENMND KRPPHIIKTIAS KT QSI
KKYSTDILGNLYEVKSKKHPQIIKKGKRPAATKKAGQAKKKKARDSKVENKTKKLRVFEAFAGI
GAQRKALEKVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEYKSVSREEMIDYLENKTLSW
NS KNPV SNGYWKRKKDDELKIIYNAIKLS EKEGNIFDIRDLYKRTLKNIDLLTYSFPC QDLS QQGI
QKGMKRGSGTRSGLLWEIERALDS TEKNDLPKYLLMENVGALLHKKNEEELNQWKQKLESLG
YQNSIEVLNAADFGS S QARRRVFMISTLNEFVELPKGDKKPKSIKKVLNKIVSEKDILNNLLKYNL
TEFKKTKSNINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRIKIKDGSNIRKMNSDETFLYIG
FDS QDGKRVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGGPSSGGKRPAATKKAGQAKKKKGS
(SEQ ID NO: 67) .. dCas9-MQ1 without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGD KRPAATKKAGQAKKKKARD S KVENKTKKLRVFEAFAGIGAQRKALE
KVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEYKSVSREEMIDYLENKTLSWNSKNPVSN
GYWKRKKDDELKIIYNAIKLSEKEGNIFDIRDLYKRTLKNIDLLTYSFPCQDLSQQGIQKGMKRG
SGTRSGLLWEIERALDSTEKNDLPKYLLMENVGALLHKKNEEELNQWKQKLESLGYQNSIEVLN
AADFGSSQARRRVFMISTLNEFVELPKGDKKPKSIKKVLNKIVSEKDILNNLLKYNLTEFKKTKS
NINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRIKIKDGSNIRKMNSDETFLYIGFDSQDGK
RVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGGPSSGGKRPAATKKAGQAKKKKGS (SEQ ID
NO: 68) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising KRAB, e.g., a KRAB domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 94 (e.g., a plasmid encoding the expression repressor) and/or 95 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 94 or 95 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
Sp-dCas9-KRAB (PL-27687) Plasmid DNA sequence:
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACA
GCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTG
GCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCAT
ATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGC
CATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAG
CTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGT
CACGACGTTGTAAAACGACGGCCAGTGAATTGACGCGTATTGGGATGGTACCTAATACGACT
CACTATAAGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCC
CCCAAGAAGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGC
ATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGG
TGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCT
GATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACC
GCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCA
ACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGA
GGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTAC
CACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGG
CCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTG
ATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGC
AGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGC
CATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCC
GGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCA
ACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTA
CGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGG
CCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGAT
CACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTG
ACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCG
ACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTA
CAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTG
AACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGA
TCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAG
GACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCT
GGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGaaatccGAGGAGACCATCACCCCCTG
GAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACC
AACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGT
ACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCC
CGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGG
AAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCG
TGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCT
GAAGATCATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGA
CATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAaACCT
ACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTG
GGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATC
CTGGACTTCCTGaaatccGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACA
GCCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCA
CGAGCACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTG
AAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCG
AGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGA
AGCGGATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGG
AGAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACAT
GTACGTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTG
CCCCAGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGG
CCCGGGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACT
GGCGGCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGC
CGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAG
ACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACG
ACGAGAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGaaatccAAGCTGGTGAGCG
ACTTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCAC
GACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGA
GCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGA
GCAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCA
AGACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGG
CGAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTG
AGCATGCCCCAGGTGAACATCGTGAAGAAaACCGAGGTGCAGACCGGCGGCTTCAGCAAGG
AGAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCC
CAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAG
GTGGAGAAGGGCAAGAGCAAGAAGCTGaaatccGTGAAGGAGCTGCTGGGCATCACCATCATG
GAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGG
TGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCG
GAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGC
AAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGG
ACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGA
GCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCTG
AGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCACC
TGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGAC
CGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCA
CCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAAGCGGCCCGCCGC
CACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGCCAGCGACGCCAAGAGCCTGACCGC
CTGGAGCCGGACCCTGGTGACCTTCAAGGACGTGTTCGTGGACTTCACCCGGGAGGAGTGG
AAGCTGCTGGACACCGCCCAGCAGATCCTGTACCGGAACGTGATGCTGGAGAACTACAAGA
ACCTGGTGAGCCTGGGCTACCAGCTGACCAAGCCCGACGTGATCCTGCGGCTGGAGAAGGG
CGAGGAGCCCTGGCTGGTGGAGCGGGAGATCCACCAGGAGACCCACCCCGACAGCGAGACC
GCCTTCGAGATCAAGAGCAGCGTGAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCG
GCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGcggccg cttaattaagctgccttctgcggggcttgccttctggccatgcccttcttctctcccttgcacctgtacctcttggtct ttgaataaagcctgagtaggaagtcta gaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa aattgtcttcttcatcgcc tgcagATCCCAATGGCGCGCCGAGCTTGGCTCGAGCATGGTCATAGCTGTTTCCTGTGTGAAAT
TGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGG
GTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGG
GAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG
TATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCG
AGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCA
GGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTG
CTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTC
GTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGA
AGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC
AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTA
TCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA
CGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT
TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTAC
GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATA
TATGAGTAAACTTGGTCTGACAGTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTT
ATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAA
ACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGT
CCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATC
ACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTT
GTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTC
ATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAA
CAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGA
ATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCAGGGATCGCAGTGGTGAGTAACC
ATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAG
CCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAG
AAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGA
CATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGC
CTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTATTGAAGC
ATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACA
AATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTA
TCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC (SEQ ID NO: 94) Sp-dCas9-KRAB (MR-28122) Expressed mRNA sequence:
AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGaaatccGAGGAGACCATCACCCCCTGGAACTTCGA
GGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGAC
AAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCG
TGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTTCCT
GAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTGACC
GTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGATCA
GCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGATCAT
CAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTGCTG
ACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAaACCTACGCCCACCT
GTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTG
AGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCC
TGaaatccGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTT
CAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAGCACAT
CGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTG
GACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGATGGCCC
GGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCGGATCG
AGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAACACCC
AGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTACGTGGA
CCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCCAGAGC
TTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCGGGGCA
AGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCGGCAGC
TGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAGCGGGG
CGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCCGGCAG
ATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACG
ACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGaaatccAAGCTGGTGAGCGACTTCCGGA
AGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACGACGCCTA
CCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAGCGAGTTC
GTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAGA
TCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAG
ATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGCGAGACCG
GCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGAGCATGCC
CCAGGTGAACATCGTGAAGAAaACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGAGCATC
CTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGT
ACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGGTGGAGAA
GGGCAAGAGCAAGAAGCTGaaatccGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGAG
CAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAG
GACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGA
TGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGCAAGTACGT
GAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGGACAACGAG
CAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCA
GCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCTGAGCGCCTA
CAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACC
CTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAAGCG
GTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTG
TACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAAGCGGCCCGCCGCCACCAAGA
AGGCCGGCCAGGCCAAGAAGAAGAAGGCCAGCGACGCCAAGAGCCTGACCGCCTGGAGCC
GGACCCTGGTGACCTTCAAGGACGTGTTCGTGGACTTCACCCGGGAGGAGTGGAAGCTGCTG
GACACCGCCCAGCAGATCCTGTACCGGAACGTGATGCTGGAGAACTACAAGAACCTGGTGA
GCCTGGGCTACCAGCTGACCAAGCCCGACGTGATCCTGCGGCTGGAGAAGGGCGAGGAGCC
CTGGCTGGTGGAGCGGGAGATCCACCAGGAGACCCACCCCGACAGCGAGACCGCCTTCGAG
ATCAAGAGCAGCGTGAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCC
AAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGcggccgcttaattaagctgc cttctgcggggcttgccttctggccatgcccttcttctctcccttgcacctgtacctcttggtctttgaataaagcctg agtaggaagtctagaaaaaaaaaaa aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa (SEQ ID
NO: 95) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 96 or 72. In some embodiments, an expression repressor described herein comprises a an amino acid sequence of SEQ ID NO: 72 or 96 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
Sp-dCas9-KRAB Protein sequence:
MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDKRPAATKKAGQAKKKKASDAKSLTAWSRTLVTFKDVFVDFTREEWK
LLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIK
SSVSGGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 96) MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLSQLGGDKRPAATKKAGQAKKKKASDAKSLTAWSRTLVTFKDVFVDFTREEWK
LLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIK
SSVSGGKRPAATKKAGQAKKKKGS (SEQ ID NO: 72) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising DNMT1, e.g., a DNMT1 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 23 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 23 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT1 (MR-29419) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCT
CAGTGGATCTGAGGACACTCGACGTGTTTAGCGGATGCGGCGGACTCTCCGAAGGCTTCCAC
CAAGCCGGAATTTCCGACACACTCTGGGCCATTGAGATGTGGGACCCCGCCGCTCAAGCCTT
CAGACTGAATAATCCCGGCTCCACCGTGTTCACCGAGGACTGCAACATTCTGCTGAAGCTGG
TGATGGCTGGCGAAACCACCAACTCTAGAGGCCAGAGGCTGCCCCAGAAGGGAGATGTGGA
AATGCTCTGTGGAGGCCCTCCTTGCCAAGGCTTCTCCGGCATGAACAGGTTCAACTCTAGAA
CATACAGCAAGTTCAAGAACTCTCTGGTCGTGAGCTTTCTGAGCTACTGCGACTACTATAGA
CCTAGGTTCTTTCTGCTGGAGAACGTGAGAAATTTCGTGTCCTTCAAGAGGAGCATGGTGCT
GAAGCTGACACTGAGGTGTCTGGTGAGGATGGGCTACCAGTGCACATTCGGAGTGCTGCAA
GCTGGCCAGTACGGCGTGGCCCAGACCAGAAGGAGGGCCATCATTCTGGCTGCTGCCCCCG
GCGAGAAACTCCCTCTGTTCCCCGAGCCCCTCCACGTGTTCGCCCCTAGAGCTTGCCAGCTG
AGCGTGGTGGTCGACGATAAGAAGTTCGTGAGCAACATCACAAGGCTGTCCAGCGGACCCT
TCAGAACCATTACCGTGAGGGATACCATGTCCGACCTCCCCGAGGTGAGGAATGGCGCCAG
CGCTCTGGAGATTTCCTACAACGGCGAACCTCAGAGCTGGTTCCAAAGGCAGCTGAGAGGC
GCTCAGTATCAGCCCATTCTGAGGGACCACATCTGCAAAGATATGAGCGCTCTGGTGGCCGC
TAGAATGAGACATATTCCTCTGGCCCCCGGCAGCGACTGGAGAGATCTGCCCAATATTGAGG
TGAGACTCAGCGACGGAACAATGGCTAGAAAACTGAGGTACACCCATCATGATAGAAAGAA
CGGAAGGAGCAGCAGCGGCGCTCTGAGAGGAGTGTGTAGCTGCGTGGAAGCTGGCAAGGCT
TGCGATCCCGCCGCTAGGCAGTTCAATACCCTCATCCCTTGGTGTCTGCCTCACACCGGCAA
CAGACACAATCATTGGGCTGGACTGTATGGAAGGCTCGAATGGGACGGCTTTTTCAGCACCA
CCGTGACCAATCCCGAACCTATGGGCAAGCAAGGAAGGGTGCTCCACCCCGAGCAGCATAG
AGTCGTGTCCGTGAGAGAATGCGCTAGAAGCCAAGGCTTCCCCGACACCTATAGACTGTTCG
GCAACATTCTGGATAAGCACAGACAAGTGGGAAATGCTGTCCCTCCTCCTCTGGCCAAGGCT
ATCGGACTGGAGATCAAGCTGTGTATGCTCGCCAAAGCTAGGGAGAGCGCTTCCGCCAAGA
TTAAGGAGGAGGAGGCCGCCAAGGACGGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCA
CCAAGAAGGCCGGTCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTA
CGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCT
TCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAA (SEQ ID NO: 23) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 34 or 69. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 34 or 69, or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT
MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDIN
RLSDYDVAAIVPQSFLKDD SIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLIT Q
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSVDLRTLDVFSGCGGLSEGFHQ
AGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEML
CGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLR
CLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKK
FVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICK
DMSALVAARMRHIPLAPGSDWRDLPNIEVRLSD GTMARKLRYTHHDRKNGRS S SGALRGVC SC
VEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVL
HPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESAS
AKIKEEEAAKDGGGGSGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 34) dCas9-DNMT without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNL SDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSVDLRTLDVFSGCGGLSEGFHQ
AGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEML
CGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLR
CLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKK
FVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICK
DMSALVAARMRHIPLAPGSDWRDLPNIEVRLSD GTMARKLRYTHHDRKNGRS S SGALRGVC SC
VEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVL
HPEQHRVV SVRECARS QGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESAS
AKIKEEEAAKDGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID NO: 69) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising DNMT3a/3L. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 24 (e.g., a nucleic acid (e.g., cDNA) encoding a fully human expression repressor) and/or 25 (e.g., a nucleic acid (e.g., cDNA) encoding a chimeric expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 24 or 25 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT 3a/3L (h) (MR-29414) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGCCGGCGGCG
GCGGCAGCGGCGGCGGCGGCAGCGGCGGCGGCGGCAGCGGCCCCAAGAAGAAGCGGAAGG
TGGCCGCCGCCGGCAGCAACCACGACCAGGAGTTCGACCCCCCCAAGGTGTACCCCCCCGT
GCCCGCCGAGAAGCGGAAGCCCATCCGGGTGCTGAGCCTGTTCGACGGCATCGCCACCGGC
CTGCTGGTGCTGAAGGACCTGGGCATCCAGGTGGACCGGTACATCGCCAGCGAGGTGTGCG
AGGACAGCATCACCGTGGGCATGGTGCGGCACCAGGGCAAGATCATGTACGTGGGCGACGT
GCGGAGCGTGACCCAGAAGCACATCCAGGAGTGGGGCCCCTTCGACCTGGTGATCGGCGGC
AGCCCCTGCAACGACCTGAGCATCGTGAACCCCGCCCGGAAGGGCCTGTACGAGGGCACCG
GCCGGCTGTTCTTCGAGTTCTACCGGCTGCTGCACGACGCCCGGCCCAAGGAGGGCGACGAC
CGGCCCTTCTTCTGGCTGTTCGAGAACGTGGTGGCCATGGGCGTGAGCGACAAGCGGGACAT
CAGCCGGTTCCTGGAGAGCAACCCCGTGATGATCGACGCCAAGGAGGTGAGCGCCGCCCAC
CGGGCCCGGTACTTCTGGGGCAACCTGCCCGGCATGAACCGGCCCCTGGCCAGCACCGTGA
ACGACAAGCTGGAGCTGCAGGAGTGCCTGGAGCACGGCCGGATCGCCAAGTTCAGCAAGGT
GCGGACCATCACCACCCGGAGCAACAGCATCAAGCAGGGCAAGGACCAGCACTTCCCCGTG
TTCATGAACGAGAAGGAGGACATCCTGTGGTGCACCGAGATGGAGCGGGTGTTCGGCTTCC
CCGTGCACTACACCGACGTGAGCAACATGAGCCGGCTGGCCCGGCAGCGGCTGCTGGGCCG
GAGCTGGAGCGTGCCCGTGATCCGGCACCTGTTCGCCCCCCTGAAGGAGTACTTCGCCTGCG
TGAGCAGCGGCAACAGCAACGCCAACAGCCGGGGCCCCAGCTTCAGCAGCGGCCTGGTGCC
CCTGAGCCTGCGGGGCAGCCACATGAATCCTCTGGAGATGTTCGAGACAGTGCCCGTGTGGA
GAAGGCAACCCGTGAGGGTGCTGAGCCTCTTCGAGGACATTAAGAAGGAGCTGACCTCTCT
GGGCTTTCTGGAATCCGGCAGCGACCCCGGCCAGCTGAAACACGTGGTGGACGTGACCGAC
ACAGTGAGGAAGGACGTGGAAGAGTGGGGCCCCTTTGACCTCGTGTATGGAGCCACACCTC
CTCTCGGCCACACATGCGATAGGCCTCCCAGCTGGTATCTCTTCCAGTTCCACAGACTGCTCC
AGTACGCCAGACCTAAGCCCGGCAGCCCCAGACCCTTCTTCTGGATGTTCGTGGACAATCTG
GTGCTGAACAAGGAGGATCTGGATGTGGCCAGCAGATTTCTGGAGATGGAACCCGTGACAA
TCCCCGACGTGCATGGCGGCTCTCTGCAGAACGCCGTGAGAGTGTGGTCCAACATCCCCGCC
ATTAGAAGCAGACACTGGGCTCTGGTGAGCGAGGAGGAACTGTCTCTGCTGGCCCAGAATA
AGCAGTCCTCCAAGCTGGCCGCCAAGTGGCCCACCAAGCTGGTGAAGAACTGCTTTCTGCCT
CTGAGGGAGTATTTCAAGTATTTCAGCACCGAACTGACCAGCAGCCTGAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACG
ACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCT
GGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAG
GAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 24) dCas9-DNMT3a/3L (m) (MR-28195) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGCCGGCGGCG
GCGGCAGCGGCGGCGGCGGCAGCGGCGGCGGCGGCAGCGGCCCCAAGAAGAAGCGGAAGG
TGGCCGCCGCCGGCAGCAACCACGACCAGGAGTTCGACCCCCCCAAGGTGTACCCCCCCGT
GCCCGCCGAGAAGCGGAAGCCCATCCGGGTGCTGAGCCTGTTCGACGGCATCGCCACCGGC
CTGCTGGTGCTGAAGGACCTGGGCATCCAGGTGGACCGGTACATCGCCAGCGAGGTGTGCG
AGGACAGCATCACCGTGGGCATGGTGCGGCACCAGGGCAAGATCATGTACGTGGGCGACGT
GCGGAGCGTGACCCAGAAGCACATCCAGGAGTGGGGCCCCTTCGACCTGGTGATCGGCGGC
AGCCCCTGCAACGACCTGAGCATCGTGAACCCCGCCCGGAAGGGCCTGTACGAGGGCACCG
GCCGGCTGTTCTTCGAGTTCTACCGGCTGCTGCACGACGCCCGGCCCAAGGAGGGCGACGAC
CGGCCCTTCTTCTGGCTGTTCGAGAACGTGGTGGCCATGGGCGTGAGCGACAAGCGGGACAT
CAGCCGGTTCCTGGAGAGCAACCCCGTGATGATCGACGCCAAGGAGGTGAGCGCCGCCCAC
CGGGCCCGGTACTTCTGGGGCAACCTGCCCGGCATGAACCGGCCCCTGGCCAGCACCGTGA
ACGACAAGCTGGAGCTGCAGGAGTGCCTGGAGCACGGCCGGATCGCCAAGTTCAGCAAGGT
GCGGACCATCACCACCCGGAGCAACAGCATCAAGCAGGGCAAGGACCAGCACTTCCCCGTG
TTCATGAACGAGAAGGAGGACATCCTGTGGTGCACCGAGATGGAGCGGGTGTTCGGCTTCC
CCGTGCACTACACCGACGTGAGCAACATGAGCCGGCTGGCCCGGCAGCGGCTGCTGGGCCG
GAGCTGGAGCGTGCCCGTGATCCGGCACCTGTTCGCCCCCCTGAAGGAGTACTTCGCCTGCG
TGAGCAGCGGCAACAGCAACGCCAACAGCCGGGGCCCCAGCTTCAGCAGCGGCCTGGTGCC
CCTGAGCCTGCGGGGCAGCCACATGGGCCCCATGGAGATCTACAAGACCGTGAGCGCCTGG
AAGCGGCAGCCCGTGCGGGTGCTGAGCCTGTTCCGGAACATCGACAAGGTGCTGAAATCCC
TGGGCTTCCTGGAGAGCGGCAGCGGCAGCGGCGGCGGCACCCTGAAGTACGTGGAGGACGT
GACCAACGTGGTGCGGCGGGACGTGGAGAAGTGGGGCCCCTTCGACCTGGTGTACGGCAGC
ACCCAGCCCCTGGGCAGCAGCTGCGACCGGTGCCCCGGCTGGTACATGTTCCAGTTCCACCG
GATCCTGCAGTACGCCCTGCCCCGGCAGGAGAGCCAGCGGCCCTTCTTCTGGATCTTCATGG
ACAACCTGCTGCTGACCGAGGACGACCAGGAGACCACCACCCGGTTCCTGCAGACCGAGGC
CGTGACCCTGCAGGACGTGCGGGGCCGGGACTACCAGAACGCCATGCGGGTGTGGAGCAAC
ATCCCCGGCCTGAAATCCAAGCACGCCCCCCTGACCCCCAAGGAGGAGGAGTACCTGCAGG
CCCAGGTGCGGAGCCGGAGCAAGCTGGACGCCCCCAAGGTGGACCTGCTGGTGAAGAACTG
CCTGCTGCCCCTGCGGGAGTACTTCAAGTACTTCAGCCAGAACAGCCTGCCCCTGAGCGGCG
GCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACC
CCTACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTG
CCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTG
AGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 25) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 35, 36, 70, or 71. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 35, 36, 70, or 71 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT3a/3L (h) MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSNHDQEFDPPKVYPPVP
AEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQ
KHIQEWGPFDLVIGGSPCNDLS IVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFEN
VVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLE
HGRIAKFS KVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLAR
QRLLGRSWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMNPLEMFETVP
VWRRQPVRVLS LFEDIKKELT SLGFLESGS DPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPP
LGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPD
VHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQS SKLAAKWPTKLVKNCFLPLREYF
KYFSTELTSSLSGGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 35) dCas9-DNMT3a/31 (m) MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSNHDQEFDPPKVYPPVP
AEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQ
KHIQEWGPFDLVIGGSPCNDLS IVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFEN
VVAMGVSDKRDISRFLESNPVMIDAKEVS AAHRARYFWGNLPGMNRPLASTVNDKLELQECLE
HGRIAKFS KVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLAR
QRLLGRSWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMNPLEMFETVP
VWRRQPVRVLS LFEDIKKELT SLGFLESGS DPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPP
LGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPD
VHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQS SKLAAKWPTKLVKNCFLPLREYF
KYFSTELTSSLSGGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 36) dCas-DNMT3a/3L (h) without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSNHDQEFDPPKVYPPVP
AEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQ
KHIQEWGPFDLVIGGSPCNDLS IVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFEN
VVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLE
HGRIAKFS KVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLAR
QRLLGRSWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMNPLEMFETVP
VWRRQPVRVLS LFEDIKKELT SLGFLESGS DPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPP
LGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPD
VHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQS SKLAAKWPTKLVKNCFLPLREYF
KYFSTELTSSLSGGKRPAATKKAGQAKKKKGS (SEQ ID NO: 70) dCas9-DNMT3a/3L (m) without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSNHDQEFDPPKVYPPVP
AEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQ
KHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFEN
VVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLE
HGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLAR
QRLLGRSWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMNPLEMFETVP
VWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPP
LGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPD
VHGGS LQNAVRVWS NIPAIRSRHWALV SEEELS LLAQNKQS SKLAAKWPTKLVKNCFLPLREYF
KYFSTELTSSLSGGKRPAATKKAGQAKKKKGS (SEQ ID NO: 71) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising G9A, e.g., a G9A
domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 26 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 26 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
G9A-dCas9 (MR-29387) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCTCGGGCGGGGGTGGCTCAGGAAA
TAGGGCTATCAGAACCGAGAAGATCATCTGTAGGGACGTGGCTAGAGGCTACGAGAACGTG
CCCATTCCTTGCGTGAATGGCGTGGATGGCGAACCTTGCCCCGAGGACTACAAATACATCTC
CGAGAACTGCGAAACCAGCACAATGAACATCGACAGAAACATCACCCACCTCCAGCACTGC
ACATGTGTGGATGACTGCTCCTCCAGCAACTGTCTGTGCGGCCAGCTCTCCATCAGATGCTG
GTACGACAAGGACGGCAGACTGCTGCAAGAGTTCAACAAGATCGAACCCCCTCTCATCTTCG
AGTGTAACCAAGCTTGCAGCTGCTGGAGAAACTGCAAGAATAGAGTGGTCCAGAGCGGCAT
CAAGGTGAGACTGCAACTGTACAGAACCGCCAAGATGGGATGGGGAGTGAGGGCTCTGCAA
ACCATTCCCCAAGGCACCTTCATCTGCGAATACGTGGGCGAACTGATCTCCGACGCCGAAGC
TGACGTGAGAGAGGACGACAGCTATCTCTTCGATCTGGACAATAAGGACGGCGAGGTGTAC
TGCATCGACGCTAGATATTACGGCAACATCTCTAGATTCATCAACCACCTCTGCGATCCCAA
CATCATTCCCGTGAGGGTGTTCATGCTGCACCAAGATCTGAGGTTCCCTAGAATCGCCTTCTT
CAGCTCTAGAGACATCAGAACCGGCGAGGAGCTGGGCTTCGATTACGGCGATAGATTCTGG
GACATCAAGTCCAAGTACTTCACATGCCAGTGCGGCAGCGAGAAGTGTAAGCACAGCGCTG
AGGCCATTGCTCTGGAGCAGTCTAGACTGGCCAGACTGGATGGAGGTGGCGGATCGGGAGA
CAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACC
GACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCA
TCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCG
GCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAG
GAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGA
GCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGA
CGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGAC
AGCACCGACAAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCG
GGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTC
ATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCG
TGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGAT
CGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTG
GGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGA
GCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGC
CGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGG
TGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCA
CCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAG
GAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCC
AGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCT
GCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGC
ATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTA
CCCCTTCCTGAAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACT
ACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGA
GACCATCACCCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTC
ATCGAGCGGATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACA
GCCTGCTGTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGA
GGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTG
TTCAAGACCAACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCG
AGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCAC
CTACCACGACCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAG
GACATCCTGGAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGG
AGCGGCTGAAAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCG
GCGGTACACCGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAG
AGCGGCAAGACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCA
GCTGATCCACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGC
CAGGGCGACAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGG
GCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCC
CGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAA
CAGCCGGGAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCT
GAAGGAGCACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTG
CAGAACGGCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACG
ACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCT
GACCCGGAGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAA
GAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTC
GACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCA
AGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCG
GATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTG
AAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCA
ACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAA
GAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGG
AAGATGATCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACA
GCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCG
GCCCCTGATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTC
GCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGC
AGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGC
CCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTAC
AGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAG
GAGCTGCTGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCT
GGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGC
CTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGG
GCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAG
AAGCTGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAG
CACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCG
ACGCCAACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGA
GCAGGCCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCA
AGTACTTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGC
CACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTG
GGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAG
AAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCT
GCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGG
TCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID
NO: 26) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 38 or 73. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 38 or 73 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
G9a-dCas9 MAPKKKRKVGIHGVPAASGGGGSGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYI
SENCETSTMNIDRNITHLQHCTCVDDCS S SNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQ
ACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVRED
D SYLFDLDNKDGEVYCIDARYYGNIS RFINHLCDPNIIPVRVFMLHQDLRFPRIAFFS S RD IRTGEE
LGFDYGDRFWDIKS KYFTCQCGSEKCKHSAEAIALEQSRLARLDGGGGSGD KKYS IGLAIGTNS V
GWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICY
LQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD
KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILS
ARLS KS RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKS NFDLAEDAKLQLS KDTYDDDLDNL
LAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL
PEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNG
SIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITP
WNFEEVVD KGASAQS FIERMTNFD KNLPNEKVLPKHS LLYEYFTVYNELTKVKYVTEGMRKPA
FLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDK
DFLDNEENED ILEDIVLTLTLFEDREMIEERLKTYAHLFDD KVMKQLKRRRYTGWGRLSRKLING
IRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGD SLHEHIANLAGSPAIKK
GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP
VENTQLQNEKLYLYYLQNGRDMYVD QELDINRLSDYDVAAIVPQSFLKDD S IDNKVLTRSD KA
RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQI
TKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNA
VVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGE
IRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIAR
KKDWDPKKYGGFD S PTVAYSVLVVAKVEKGKS KKLKSVKELLGITIMERS SFEKNPIDFLEAKG
YKEVKKDLIIKLPKYSLFELENGRKRMLAS AGELQKGNELALPSKYVNFLYLASHYEKLKGSPE
DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLT
NLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS QLGGDSGGKRPAATKKAG
QAKKKKGSYPYDVPDYA (SEQ ID NO: 38) G9A-dCas9 without HA tag MAPKKKRKVGIHGVPAASGGGGSGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYI
SENCETSTMNIDRNITHLQHCTCVDDCS SSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQ
ACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVRED
D SYLFDLDNKDGEVYCIDARYYGNIS RFINHLCDPNIIPVRVFMLHQDLRFPRIAFFS S RD IRTGEE
LGFDYGDRFWDIKS KYFTCQCGSEKCKHSAEAIALEQSRLARLDGGGGSGD KKYS IGLAIGTNS V
GWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICY
LQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD
KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILS
ARLS KSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKS NFDLAEDAKLQLSKDTYDDDLDNL
LAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL
PEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNG
SIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITP
WNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPA
FLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDK
DFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLING
IRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKK
GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP
VENTQLQNEKLYLYYLQNGRDMYVD QELDINRLSDYDVAAIVPQSFLKDD S IDNKVLTRSDKA
RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQI
TKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNA
VVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGE
IRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIAR
KKDWDPKKYGGFD S PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERS SFEKNPIDFLEAKG
YKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE
DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLT
NLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS QLGGDSGGKRPAATKKAG
QAKKKKGS (SEQ ID NO: 73) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising HDAC8, e.g., a HDAC8 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 27 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 27 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-HDAC8 (MR-29439) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCT
CAGAGGAGCCCGAGGAGCCCGCCGATAGCGGACAATCTCTGGTGCCCGTCTACATCTACAG
CCCCGAATATGTGAGCATGTGTGATTCCCTCGCCAAGATCCCTAAGAGAGCCAGCATGGTGC
ATTCTCTGATCGAGGCCTACGCTCTGCATAAGCAAATGAGGATCGTGAAGCCCAAGGTCGCC
AGCATGGAAGAGATGGCCACCTTTCACACCGATGCCTACCTCCAACATCTCCAGAAGGTGTC
CCAAGAGGGCGACGACGACCACCCCGACTCCATTGAGTACGGACTGGGCTATGATTGCCCC
GCCACCGAGGGCATCTTTGACTATGCCGCCGCTATCGGCGGAGCTACCATCACAGCCGCCCA
GTGTCTGATTGATGGCATGTGCAAGGTCGCCATCAACTGGTCCGGAGGCTGGCATCATGCCA
AGAAGGATGAGGCCTCCGGCTTCTGTTATCTGAATGACGCCGTGCTGGGCATTCTGAGACTG
AGGAGGAAATTCGAGAGGATTCTGTACGTGGATCTGGATCTGCATCACGGAGATGGAGTCG
AAGATGCCTTCAGCTTCACCAGCAAGGTGATGACAGTCTCTCTGCACAAGTTCTCCCCCGGC
TTCTTTCCCGGAACCGGCGACGTGTCCGACGTGGGACTGGGCAAGGGAAGGTACTACAGCG
TGAACGTGCCCATTCAAGACGGCATCCAAGACGAGAAGTACTACCAGATCTGCGAGTCCGT
GCTCAAGGAGGTCTACCAAGCCTTCAATCCTAAGGCTGTCGTGCTCCAACTGGGAGCTGATA
CCATTGCTGGCGATCCCATGTGCAGCTTCAATATGACACCCGTCGGAATCGGCAAGTGCCTC
AAGTACATCCTCCAGTGGCAGCTCGCCACCCTCATTCTCGGAGGAGGCGGATACAATCTGGC
TAATACCGCCAGATGCTGGACCTATCTGACCGGCGTGATTCTGGGCAAAACACTGAGCAGCG
AAATCCCCGACCACGAGTTTTTCACCGCTTACGGCCCCGACTACGTGCTGGAGATCACCCCC
AGCTGCAGACCCGATAGAAACGAACCCCATAGAATCCAGCAAATTCTGAACTATATCAAGG
GCAACCTCAAGCACGTCGTGGGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCACCAAGAA
GGCCGGTCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGA
GCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCC
CTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA (SEQ ID NO: 27) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 39 or 74. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 39 or 74 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-HDAC8 MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNL SDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSEEPEEPAD SGQSLVPVYIYS PE
YVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEG
DDDHPD S IEYGLGYDCPATEGIFDYAAAIGGATITAAQCLID GMCKVAINWSGGWHHAKKDEAS
GFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVS
DVGLGKGRYYS VNVPIQDGIQDEKYYQICES VLKEVYQAFNPKAVVLQLGADTIAGDPMC SFN
MTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLS SEIPDHEFFTAYGPD
YVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVVGGGGSGKRPAATKKAGQAKKKKGSYPYDVP
DYA (SEQ ID NO: 39) dCas9-HDAC8 without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
.. QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGD S GGKRPAAT KKAGQAKKKKS GGGGSEEPEEPAD SGQSLVPVYIYS PE
YVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEG
DDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEAS
GFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVS
DVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFN
MTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPD
YVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVVGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID
NO: 74) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising LSD1, e.g., a LSD1 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 28 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 28 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-LSD1 (MR-29129) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGAGAGCGGCGGCGGCA
GCCTCTCCGGAAAGAAAGCCGCCGCTGCCGCTGCCGCCGCTGCTGCTGCCGCTACCGGCACA
GAAGCTGGCCCCGGAACAGCTGGCGGAAGCGAAAACGGAAGCGAAGTGGCTGCCCAGCCC
GCCGGACTGTCCGGACCCGCCGAGGTGGGACCCGGCGCTGTCGGCGAAAGGACCCCCAGAA
AAAAGGAGCCTCCTAGAGCCAGCCCTCCCGGCGGACTCGCCGAACCTCCCGGCAGCGCTGG
ACCTCAAGCCGGACCTACAGTGGTGCCCGGCAGCGCTACACCTATGGAGACCGGCATCGCT
GAGACCCCCGAGGGAAGGAGAACCAGCAGAAGGAAGAGAGCCAAGGTGGAGTACAGAGAG
ATGGATGAGTCTCTGGCTAACCTCAGCGAGGACGAGTACTACTCCGAGGAGGAAAGGAATG
CCAAGGCCGAGAAGGAGAAGAAGCTGCCCCCTCCTCCCCCTCAAGCCCCCCCCGAGGAGGA
GAACGAGTCCGAGCCCGAGGAACCCAGCGGAGTGGAAGGAGCCGCCTTTCAGTCCAGACTG
CCCCACGACAGAATGACATCCCAAGAGGCCGCTTGCTTTCCCGACATTATTTCCGGCCCTCA
GCAGACCCAGAAGGTGTTTCTGTTCATTAGAAATAGAACACTCCAGCTGTGGCTCGACAACC
CCAAGATCCAGCTGACCTTCGAGGCTACCCTCCAACAGCTGGAGGCCCCCTACAATAGCGAT
ACCGTGCTGGTGCACAGAGTGCACAGCTATCTGGAGAGGCACGGCCTCATTAACTTCGGCAT
TTACAAGCGGATCAAGCCCCTGCCCACCAAGAAAACAGGCAAGGTGATCATCATCGGCAGC
GGCGTGAGCGGCCTGGCCGCCGCCCGGCAGCTGCAGAGCTTCGGCATGGACGTGACCCTGC
TGGAGGCCCGGGACCGGGTGGGCGGCCGGGTGGCCACCTTCCGGAAGGGCAACTACGTGGC
CGACCTGGGCGCCATGGTGGTGACCGGCCTGGGCGGCAACCCCATGGCCGTGGTGAGCAAG
CAGGTGAACATGGAGCTGGCCAAGATCAAGCAGAAGTGCCCCCTGTACGAGGCCAACGGCC
AGGCCGTGCCCAAGGAGAAGGACGAGATGGTGGAGCAGGAGTTCAACCGGCTGCTGGAGG
CCACCAGCTACCTGAGCCACCAGCTGGACTTCAACGTGCTGAACAACAAGCACGTGAGCCT
GGGCCAGGCCCTGGAGGTGGTGATCCAGCTGCAGGAGAAGCACGTGAAGGACGAGCAGATC
GAGCACTGGAAGAAGATCGTGAAAACACAGGAGGAGCTGAAGGAGCTGCTGAACAAGATG
GTGAACCTGAAGGAGAAGATCAAGGAGCTGCACCAGCAGTACAAGGAGGCCAGCGAGGTG
AAGCCCCCCCGGGACATCACCGCCGAGTTCCTGGTCAAAAGCAAGCACCGGGACCTGACCG
CCCTGTGCAAGGAGTACGACGAGCTGGCCGAGACCCAGGGCAAGCTGGAGGAGAAGCTGCA
GGAGCTGGAGGCCAACCCCCCCAGCGACGTGTACCTGAGCAGCCGGGACCGGCAGATCCTG
GACTGGCACTTCGCCAACCTGGAGTTCGCCAACGCCACCCCCCTGAGCACCCTGAGCCTGAA
GCACTGGGACCAGGACGACGACTTCGAGTTCACCGGCAGCCACCTGACCGTGCGGAACGGC
TACAGCTGCGTGCCCGTGGCCCTGGCCGAGGGCCTGGACATCAAGCTGAACACCGCCGTGC
GGCAGGTGCGGTACACCGCCAGCGGCTGCGAGGTGATCGCCGTGAACACCCGGAGCACCAG
CCAGACCTTCATCTACAAGTGCGACGCCGTGCTGTGCACCCTGCCCCTGGGCGTGCTGAAGC
AGCAGCCCCCCGCCGTGCAGTTCGTGCCCCCCCTGCCCGAGTGGAAAACAAGCGCCGTGCA
GCGGATGGGCTTCGGCAACCTGAACAAGGTGGTGCTGTGCTTCGACCGGGTGTTCTGGGACC
CCAGCGTGAACCTGTTCGGCCACGTGGGCAGCACCACCGCCAGCCGGGGCGAGCTGTTCCTG
TTCTGGAACCTGTACAAGGCCCCCATCCTGCTGGCCCTGGTGGCCGGCGAGGCCGCCGGCAT
CATGGAGAACATCAGCGACGACGTGATCGTGGGCCGGTGCCTGGCCATCCTGAAGGGCATC
TTCGGCAGCAGCGCCGTGCCCCAGCCCAAGGAGACCGTGGTGAGCCGGTGGCGGGCCGACC
CCTGGGCCCGGGGCAGCTACAGCTACGTGGCCGCCGGCAGCAGCGGCAACGACTACGACCT
GATGGCCCAGCCCATCACCCCCGGCCCCAGCATCCCCGGCGCCCCCCAGCCCATCCCCCGGC
TGTTCTTCGCCGGCGAGCACACCATCCGGAACTACCCCGCCACCGTGCACGGCGCCCTGCTG
AGCGGCCTGCGGGAGGCAGGACGGATCGCCGACCAGTTCCTGGGCGCCATGTACACCCTGC
CCCGGCAGGCCACCCCCGGCGTGCCCGCCCAGCAGAGCCCCAGCATGAGCGGCGGCAAGCG
GCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGA
CGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTG
GCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGG
AAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 28) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 40 or 75. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 40 or 75, or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-LSD1 MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDD SIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLIT Q
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLD KVLSAYNKHRD KPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYT STKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKESGGGSLSGKKAAAAAAAAAAAATGT
EAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASPPGGLAEPPGSAGPQA
GPTVVPGSATPMETGIAETPEGRRTS RRKRAKVEYREMDES LANLSEDEYYSEEERNAKAEKEK
KLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTS QEAACFPDIISGPQQTQKVFLFIRNR
TLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVI
IIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAV
V SKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATS YLSHQLDFNVLNNKHVS
LGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPP
RD ITAEFLVKS KHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLS SRDRQILDWHFANL
EFANATPLS TLS LKHWD QDDDFEFTGSHLTVRNGYS CVPVALAEGLDIKLNTAVRQVRYTAS GC
EVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVL
CFDRVFWDPS VNLFGHVGSTTAS RGELFLFWNLYKAPILLALVAGEAAGIMENIS DDVIVGRCLA
ILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGS SGNDYDLMAQPITPGPSIPGAPQPIPR
LFFAGEHTIRNYPATVHGALLSGLREAGRIAD QFLGAMYTLPRQATPGVPA QQS PS MSGGKRPA
ATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 40) dCas9-LSD1 without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNS VGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYL QEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNL SDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDIN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKESGGGSLSGKKAAAAAAAAAAAATGT
EAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASPPGGLAEPPGSAGPQA
GPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLANLSEDEYYSEEERNAKAEKEK
KLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTS QEAACFPDIISGPQQTQKVFLFIRNR
TLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVI
IIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAV
VSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKHVS
LGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPP
RD ITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLS SRDRQILDWHFANL
EFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGC
EVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVL
CFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLA
ILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPR
LFFAGEHTIRNYPATVHGALLSGLREAGRIAD QFLGAMYTLPRQATPGVPA QQSPS MSGGKRPA
ATKKAGQAKKKKGS (SEQ ID NO: 75) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising EZH2, e.g., a EZH2 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 29 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 29 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
EZH2-dCas9 (MR-28938) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCGGCAGCGGCGGCAGCGGCCAGACCGGCAAGAAGAGCGAGAAGG
GCCCCGTGTGCTGGCGGAAGCGGGTGAAGAGCGAGTACATGCGGCTGCGGCAGCTGAAGCG
GTTCCGGCGGGCCGACGAGGTGAAGAGCATGTTCAGCAGCAACCGGCAGAAGATCCTGGAG
CGGACCGAGATCCTGAACCAGGAGTGGAAGCAGCGGCGAATCCAGCCCGTGCACATCCTGA
CCAGCGTGAGCAGCCTGCGGGGCACCCGGGAGTGCAGCGTGACCAGCGACCTGGACTTCCC
CACCCAGGTGATCCCCCTAAAGACCCTGAACGCCGTGGCCAGCGTGCCCATCATGTACAGCT
GGAGCCCCCTGCAGCAGAACTTCATGGTGGAGGACGAGACCGTGCTGCACAACATCCCCTA
CATGGGCGACGAGGTGCTGGACCAGGACGGCACCTTCATCGAGGAGCTGATCAAGAACTAC
GACGGCAAGGTGCACGGCGACCGGGAGTGCGGCTTCATCAACGACGAGATCTTCGTGGAGC
TGGTGAACGCCCTGGGCCAGTACAACGACGACGACGACGACGACGACGGCGACGACCCCGA
GGAGCGGGAGGAGAAGCAGAAGGACCTGGAGGACCACCGGGACGACAAGGAGAGCCGGCC
CCCCCGGAAGTTCCCCAGCGACAAGATCTTCGAGGCCATCAGCAGCATGTTCCCCGACAAGG
GCACCGCCGAGGAGCTGAAGGAGAAGTACAAGGAGCTGACCGAGCAGCAGCTGCCCGGCG
CCCTGCCCCCCGAGTGCACCCCCAACATCGACGGCCCCAACGCCAAGAGCGTGCAGCGGGA
GCAGAGCCTGCACAGCTTCCACACCCTGTTCTGCCGGCGGTGCTTCAAGTACGACTGCTTCC
TGCACCCCTTCCACGCCACCCCCAACACCTACAAGCGGAAGAACACCGAGACCGCCCTGGA
CAACAAGCCCTGCGGCCCCCAGTGCTACCAGCACCTGGAGGGCGCCAAGGAGTTCGCCGCC
GCCCTGACCGCCGAGCGGATCAAGACCCCCCCCAAGCGGCCCGGCGGCCGGCGGCGGGGCC
GGCTGCCCAACAACAGCAGCCGGCCCAGCACCCCCACCATCAACGTGCTGGAGAGCAAGGA
CACCGACAGCGACCGGGAGGCCGGCACCGAGACCGGCGGCGAGAACAACGACAAGGAGGA
GGAGGAGAAGAAGGACGAGACCAGCAGCAGCAGCGAGGCCAACAGCCGGTGCCAGACCCC
CATCAAGATGAAGCCCAACATCGAGCCCCCCGAGAACGTGGAGTGGAGCGGCGCCGAGGCC
AGCATGTTCCGGGTGCTGATCGGCACCTACTACGACAACTTCTGCGCCATCGCCCGGCTGAT
CGGCACCAAGACCTGCCGGCAGGTGTACGAGTTCCGGGTGAAGGAGAGCAGCATCATCGCC
CCCGCCCCCGCCGAGGACGTGGACACCCCCCCCCGGAAGAAGAAGCGGAAGCACCGGCTGT
GGGCCGCCCACTGCCGGAAGATCCAGCTGAAGAAGGACGGCAGCAGCAACCACGTGTACAA
CTACCAGCCCTGCGACCACCCCCGGCAGCCCTGCGACAGCAGCTGCCCCTGCGTGATCGCCC
AGAACTTCTGCGAGAAGTTCTGCCAGTGCAGCAGCGAGTGCCAGAACCGGTTCCCCGGCTGC
CGGTGCAAGGCCCAGTGCAACACCAAGCAGTGCCCCTGCTACCTGGCCGTGCGGGAGTGCG
ACCCCGACCTGTGCCTGACCTGCGGCGCCGCCGACCACTGGGACAGCAAGAACGTGAGCTG
CAAGAACTGCAGCATCCAGCGGGGCAGCAAGAAGCACCTGCTGCTGGCCCCCAGCGACGTG
GCCGGCTGGGGCATCTTCATCAAGGACCCCGTGCAGAAGAACGAGTTCATCAGCGAGTACT
GCGGCGAGATCATCAGCCAGGACGAGGCCGACCGGCGGGGCAAGGTGTACGACAAGTACAT
GTGCAGCTTCCTGTTCAACCTGAACAACGACTTCGTGGTGGACGCCACCCGGAAGGGCAACA
AGATCCGGTTCGCCAACCACAGCGTGAACCCCAACTGCTACGCCAAGGTGATGATGGTGAA
CGGCGACCACCGGATCGGCATCTTCGCCAAGCGGGCCATCCAGACCGGCGAGGAGCTGTTC
TTCGACTACCGGTACAGCCAGGCCGACGCCCTGAAGTACGTGGGCATCGAGCGGGAGATGG
AGATCCCCAGCACCGGCGGCAGCGGCGGCAGCGGCGGCAGCGGCGGCAGCGGCGGCAGCG
GCCGACCCGACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGC
CGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGAC
CGGCACAGCATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCG
AGGCCACCCGGCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTG
CTACCTGCAGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGC
TGGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAA
CATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAG
CTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGAT
CAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGAC
AAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGC
CAGCGGCGTGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAG
AACCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCC
TGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCT
GCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGAC
CAGTACGCCGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACAT
CCTGCGGGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTAC
GACGAGCACCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGA
AGTACAAGGAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGG
CGCCAGCCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACC
GAGGAGCTGCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACA
ACGGCAGCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGA
GGACTTCTACCCCTTCCTGAAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGG
ATCCCCTACTACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAA
ATCCGAGGAGACCATCACCCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCC
CAGAGCTTCATCGAGCGGATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGC
CCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTA
CGTGACCGAGGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTG
GACCTGCTGTTCAAGACCAACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCA
AGAAGATCGAGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAG
CCTGGGCACCTACCACGACCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAG
GAGAACGAGGACATCCTGGAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGA
TGATCGAGGAGCGGCTGAAAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCT
GAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGG
GACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGA
ACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCA
GGTGAGCGGCCAGGGCGACAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCC
ATCAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCC
GGCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGG
GCCAGAAGAACAGCCGGGAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCA
GCCAGATCCTGAAGGAGCACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCT
GTACTACCTGCAGAACGGCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTG
AGCGACTACGACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACA
ACAAGGTGCTGACCCGGAGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGG
AGGTGGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCA
GCGGAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCC
GGCTTCATCAAGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCC
TGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGG
TGATCACCCTGAAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTG
CGGGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCG
CCCTGATCAAGAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTA
CGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTA
CTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGA
TCCGGAAGCGGCCCCTGATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGG
CCGGGACTTCGCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAA
ACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACA
AGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCAC
CGTGGCCTACAGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAA
ATCCGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCC
ATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGC
CCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGA
GCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCC
AGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGG
AGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGT
GATCCTGGCCGACGCCAACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAG
CCCATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCC
CGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAG
GTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCT
GAGCCAGCTGGGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCA
GGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGCGGCCGCT
TAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTG
TACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AA (SEQ ID NO: 29) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 41 or 76. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 41 or 76 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
EZH2-dCas9 MAPKKKRKVGGSGGSGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQK
ILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSP
LQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALG
QYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEK
YKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKR
KNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVL
ESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHC
RKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCN
TKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDP
VQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIPSTGGSGGSGGSGG
SGGSGRPDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA
EATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQ
TYNQLFEENPINASGVDAKAILSARLS KS RRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNF
DLAEDAKLQLS KDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGASQEEFYKFIKPILEKMDGT
EELLVKLNREDLLRKQRTFDNGSIPH QIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
PLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM
KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNS
RERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVAAI
VPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKT
EV QTGGFS KESILPKRNS D KLIARKKDWDPKKYGGFD SPTVAYSVLVVAKVEKGKS KKLKSVKE
LLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLAS AGELQKGNELAL
PS KYVNFLYLAS HYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQIS EFS KRVILADANLD KVLS A
YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGDSGGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 41) EZH2-dCas9 without HA tag MAPKKKRKVGGSGGSGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFS SNRQK
ILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSP
LQQNFMVEDETVLHNIPYMGDEVLD QDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALG
QYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEK
YKELTEQQLPGALPPECTPNIDGPNAKS VQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKR
KNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNS SRPSTPTINVL
ES KDTD SDREAGTETGGENND KEEEEKKDET S S S SEANS RC QTPIKMKPNIEPPENVEWS GAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHC
RKIQLKKDGS SNHVYNYQPCDHPRQPCDS SCPCVIAQNFCEKFCQCS SEC QNRFPGCRCKAQCN
TKQCPCYLAVRECDPDLCLTCGAADHWD S KNV SC KNC SIQRGS KKHLLLAPSDVAGWGIFIKDP
V QKNEFISEYCGEIIS QDEADRRGKVYD KYMCS FLFNLNNDFVVDATRKGN KIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIPSTGGSGGSGGSGG
SGGSGRPDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA
EATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQ
TYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNF
DLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKFIKPILEKMDGT
EELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
PLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNAS LGTYHDLLKIIKDKDFLDNEENEDILED IVLTLTLFEDREMIEERLKTYAHLFDDKVM
KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNS
RERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVAAI
VPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKT
EVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKE
LLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLAS AGELQKGNELAL
PSKYVNFLYLAS HYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQIS EFS KRVILADANLDKVLS A
YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGDSGGKRPAATKKAGQAKKKKGS (SEQ ID NO: 76) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a first repressor domain comprising EZH2 e.g., a EZH2 domain and a second repressor domain comprising KRAB, e.g., a KRAB domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 30 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 30 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
EZH2- dCas9-KRAB (MR-29448) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCGGCAGCGGCGGCAGCGGCCAGACCGGCAAGAAGAGCGAGAAGG
GCCCCGTGTGCTGGCGGAAGCGGGTGAAGAGCGAGTACATGCGGCTGCGGCAGCTGAAGCG
GTTCCGGCGGGCCGACGAGGTGAAGAGCATGTTCAGCAGCAACCGGCAGAAGATCCTGGAG
CGGACCGAGATCCTGAACCAGGAGTGGAAGCAGCGGCGAATCCAGCCCGTGCACATCCTGA
CCAGCGTGAGCAGCCTGCGGGGCACCCGGGAGTGCAGCGTGACCAGCGACCTGGACTTCCC
CACCCAGGTGATCCCCCTAAAGACCCTGAACGCCGTGGCCAGCGTGCCCATCATGTACAGCT
GGAGCCCCCTGCAGCAGAACTTCATGGTGGAGGACGAGACCGTGCTGCACAACATCCCCTA
CATGGGCGACGAGGTGCTGGACCAGGACGGCACCTTCATCGAGGAGCTGATCAAGAACTAC
GACGGCAAGGTGCACGGCGACCGGGAGTGCGGCTTCATCAACGACGAGATCTTCGTGGAGC
TGGTGAACGCCCTGGGCCAGTACAACGACGACGACGACGACGACGACGGCGACGACCCCGA
GGAGCGGGAGGAGAAGCAGAAGGACCTGGAGGACCACCGGGACGACAAGGAGAGCCGGCC
CCCCCGGAAGTTCCCCAGCGACAAGATCTTCGAGGCCATCAGCAGCATGTTCCCCGACAAGG
GCACCGCCGAGGAGCTGAAGGAGAAGTACAAGGAGCTGACCGAGCAGCAGCTGCCCGGCG
CCCTGCCCCCCGAGTGCACCCCCAACATCGACGGCCCCAACGCCAAGAGCGTGCAGCGGGA
GCAGAGCCTGCACAGCTTCCACACCCTGTTCTGCCGGCGGTGCTTCAAGTACGACTGCTTCC
TGCACCCCTTCCACGCCACCCCCAACACCTACAAGCGGAAGAACACCGAGACCGCCCTGGA
CAACAAGCCCTGCGGCCCCCAGTGCTACCAGCACCTGGAGGGCGCCAAGGAGTTCGCCGCC
GCCCTGACCGCCGAGCGGATCAAGACCCCCCCCAAGCGGCCCGGCGGCCGGCGGCGGGGCC
GGCTGCCCAACAACAGCAGCCGGCCCAGCACCCCCACCATCAACGTGCTGGAGAGCAAGGA
CACCGACAGCGACCGGGAGGCCGGCACCGAGACCGGCGGCGAGAACAACGACAAGGAGGA
GGAGGAGAAGAAGGACGAGACCAGCAGCAGCAGCGAGGCCAACAGCCGGTGCCAGACCCC
CATCAAGATGAAGCCCAACATCGAGCCCCCCGAGAACGTGGAGTGGAGCGGCGCCGAGGCC
AGCATGTTCCGGGTGCTGATCGGCACCTACTACGACAACTTCTGCGCCATCGCCCGGCTGAT
CGGCACCAAGACCTGCCGGCAGGTGTACGAGTTCCGGGTGAAGGAGAGCAGCATCATCGCC
CCCGCCCCCGCCGAGGACGTGGACACCCCCCCCCGGAAGAAGAAGCGGAAGCACCGGCTGT
GGGCCGCCCACTGCCGGAAGATCCAGCTGAAGAAGGACGGCAGCAGCAACCACGTGTACAA
CTACCAGCCCTGCGACCACCCCCGGCAGCCCTGCGACAGCAGCTGCCCCTGCGTGATCGCCC
AGAACTTCTGCGAGAAGTTCTGCCAGTGCAGCAGCGAGTGCCAGAACCGGTTCCCCGGCTGC
CGGTGCAAGGCCCAGTGCAACACCAAGCAGTGCCCCTGCTACCTGGCCGTGCGGGAGTGCG
ACCCCGACCTGTGCCTGACCTGCGGCGCCGCCGACCACTGGGACAGCAAGAACGTGAGCTG
CAAGAACTGCAGCATCCAGCGGGGCAGCAAGAAGCACCTGCTGCTGGCCCCCAGCGACGTG
GCCGGCTGGGGCATCTTCATCAAGGACCCCGTGCAGAAGAACGAGTTCATCAGCGAGTACT
GCGGCGAGATCATCAGCCAGGACGAGGCCGACCGGCGGGGCAAGGTGTACGACAAGTACAT
GTGCAGCTTCCTGTTCAACCTGAACAACGACTTCGTGGTGGACGCCACCCGGAAGGGCAACA
AGATCCGGTTCGCCAACCACAGCGTGAACCCCAACTGCTACGCCAAGGTGATGATGGTGAA
CGGCGACCACCGGATCGGCATCTTCGCCAAGCGGGCCATCCAGACCGGCGAGGAGCTGTTC
TTCGACTACCGGTACAGCCAGGCCGACGCCCTGAAGTACGTGGGCATCGAGCGGGAGATGG
AGATCCCCAGCACCGGCGGCAGCGGCGGCAGCGGCGGCAGCGGCGGCAGCGGCGGCAGCG
GCCGACCCGACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGC
CGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGAC
CGGCACAGCATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCG
AGGCCACCCGGCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTG
CTACCTGCAGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGC
TGGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAA
CATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAG
CTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGAT
CAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGAC
AAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGC
CAGCGGCGTGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAG
AACCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCC
TGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCT
GCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGAC
CAGTACGCCGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACAT
CCTGCGGGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTAC
GACGAGCACCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGA
AGTACAAGGAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGG
CGCCAGCCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACC
GAGGAGCTGCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACA
ACGGCAGCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGA
GGACTTCTACCCCTTCCTGAAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGG
ATCCCCTACTACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAA
ATCCGAGGAGACCATCACCCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCC
CAGAGCTTCATCGAGCGGATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGC
CCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTA
CGTGACCGAGGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTG
GACCTGCTGTTCAAGACCAACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCA
AGAAGATCGAGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAG
CCTGGGCACCTACCACGACCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAG
GAGAACGAGGACATCCTGGAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGA
TGATCGAGGAGCGGCTGAAAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCT
GAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGG
GACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGA
ACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCA
GGTGAGCGGCCAGGGCGACAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCC
ATCAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCC
GGCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGG
GCCAGAAGAACAGCCGGGAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCA
GCCAGATCCTGAAGGAGCACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCT
GTACTACCTGCAGAACGGCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTG
AGCGACTACGACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACA
ACAAGGTGCTGACCCGGAGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGG
AGGTGGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCA
GCGGAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCC
GGCTTCATCAAGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCC
TGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGG
TGATCACCCTGAAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTG
CGGGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCG
CCCTGATCAAGAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTA
CGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTA
CTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGA
TCCGGAAGCGGCCCCTGATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGG
CCGGGACTTCGCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAA
ACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACA
AGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCAC
CGTGGCCTACAGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAA
ATCCGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCC
ATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGC
CCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGA
GCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCC
AGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGG
AGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGT
GATCCTGGCCGACGCCAACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAG
CCCATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCC
CGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAG
GTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCT
GAGCCAGCTGGGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCA
GGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCTCAGACGCTAAGTCTCTGACCGCTTGGAGC
AGAACACTGGTCACCTTCAAGGACGTGTTCGTCGACTTCACAAGAGAGGAGTGGAAACTGC
TGGACACCGCCCAGCAGATCCTCTATAGAAACGTCATGCTGGAGAACTACAAGAATCTGGT
GTCTCTGGGCTACCAGCTGACCAAGCCCGACGTGATTCTGAGGCTGGAGAAGGGCGAGGAG
CCTTGGCTGGTGGAGAGAGAGATCCACCAAGAAACCCACCCCGACAGCGAAACCGCCTTCG
AGATCAAGAGCAGCGTGGGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCACCAAGAAGG
CCGGTCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGC
GGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTT
GCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA (SEQ ID NO: 30) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 42 or 77. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 42 or 77 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
EZH2-dCas9-KRAB
MAPKKKRKVGGSGGSGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQK
ILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSP
LQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALG
QYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEK
YKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKR
KNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVL
ESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHC
RKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCN
TKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDP
V QKNEFISEYCGEIIS QDEADRRGKVYD KYMCS FLFNLNNDFVVDATRKGN KIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIPSTGGSGGSGGSGG
SGGSGRPDKKYSIGLAIGTNSVGWAVITDEYKVPS KKFKVLGNTDRHSIKKNLIGALLFDSGETA
EATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQ
TYNQLFEENPINASGVDAKAILSARLS KS RRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNF
DLAEDAKLQLS KDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGASQEEFYKFIKPILEKMDGT
EELLVKLNREDLLRKQRTFDNGSIPH QIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
PLARGNSRFAWMTRKSEETITPWNFEEVVD KGASAQS FIERMTNFD KNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM
KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNS
RERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVAAI
VPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKT
EV QTGGFS KESILPKRNS D KLIARKKDWDPKKYGGFD SPTVAYSVLVVAKVEKGKS KKLKSVKE
LLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLAS AGELQKGNELAL
PS KYVNFLYLAS HYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQIS EFS KRVILADANLD KVLS A
YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGDSGGKRPAATKKAGQAKKKKSGGGGSDAKSLTAWSRTLVTFKDVFVDFTREEWKL
LDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIKS
SVGGGGSGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 42) EZH2-dCas9-KRAB without HA tag MAPKKKRKVGGSGGSGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFS SNRQK
ILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSP
LQQNFMVEDETVLHNIPYMGDEVLD QDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALG
QYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEK
YKELTEQQLPGALPPECTPNIDGPNAKS VQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKR
KNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNS SRPSTPTINVL
ESKDTDSDREAGTETGGENNDKEEEEKKDETSSS SEANSRCQTPIKMKPNIEPPENVEWSGAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHC
RKIQLKKDGS SNHVYNYQPCDHPRQPCDS SCPCVIAQNFCEKFCQCS SECQNRFPGCRCKAQCN
TKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDP
V QKNEFISEYCGEIIS QDEADRRGKVYD KYMCS FLFNLNNDFVVDATRKGNKIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIPSTGGSGGSGGSGG
SGGSGRPDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA
EATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQ
TYNQLFEENPINAS GVDAKAILS ARLS KS RRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNF
DLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKFIKPILEKMDGT
EELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
PLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM
KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNS
RERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVAAI
VPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKT
EVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKE
LLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLAS AGELQKGNELAL
PSKYVNFLYLAS HYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQIS EFS KRVILADANLD KVLS A
YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGDSGGKRPAATKKAGQAKKKKSGGGGSDAKSLTAWSRTLVTFKDVFVDFTREEWKL
LDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIKS
SVGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID NO: 77) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a first repressor domain comprising G9A e.g., a G9A domain and a second repressor domain comprising KRAB, e.g., a KRAB domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 31 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 31 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
G9A-dCas9-KRAB (MR-29442) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCTCGGGCGGGGGTGGCTCAGGAAG
CGCCGCTATCGCCGAAGTGCTGCTGAATGCCAGATGCGATCTGCATGCCGTGAACTACCACG
GCGACACCCCTCTGCATATCGCCGCTAGAGAGAGCTACCATGACTGTGTGCTGCTGTTTCTG
AGCAGAGGCGCCAATCCCGAGCTCAGAAACAAAGAGGGCGACACCGCTTGGGATCTGACAC
CCGAGAGATCCGACGTGTGGTTCGCTCTGCAACTGAATAGAAAACTGAGACTGGGCGTCGG
CAATAGGGCCATTAGAACCGAGAAGATCATCTGTAGGGACGTGGCTAGGGGCTACGAGAAC
GTGCCCATCCCTTGTGTGAACGGAGTGGATGGAGAGCCTTGCCCCGAGGATTACAAATACAT
CAGCGAGAACTGCGAAACCTCCACCATGAATATCGATAGAAACATTACACACCTCCAGCAC
TGTACATGCGTGGACGATTGCAGCAGCAGCAACTGTCTGTGCGGCCAACTGAGCATCAGATG
CTGGTACGACAAGGATGGCAGACTGCTGCAAGAGTTCAACAAGATCGAACCCCCTCTGATCT
TCGAGTGTAACCAAGCTTGCAGCTGTTGGAGGAACTGCAAGAATAGGGTCGTGCAGTCCGG
AATCAAGGTGAGACTGCAGCTGTATAGAACAGCTAAGATGGGATGGGGAGTCAGAGCTCTG
CAGACCATCCCCCAAGGCACATTCATCTGTGAGTACGTCGGCGAACTCATCAGCGACGCTGA
GGCCGATGTGAGGGAGGACGACAGCTATCTCTTCGACCTCGACAACAAGGACGGCGAGGTG
TACTGCATCGACGCTAGATATTACGGCAACATCAGCAGATTCATCAACCACCTCTGCGACCC
CAATATCATCCCCGTGAGAGTGTTCATGCTCCATCAAGATCTGAGATTCCCTAGGATCGCCTT
CTTCAGCTCTAGAGACATTAGAACCGGCGAGGAGCTGGGATTCGACTACGGCGACAGGTTCT
GGGACATCAAGAGCAAGTACTTCACATGCCAATGCGGCAGCGAGAAATGCAAGCATAGCGC
CGAGGCCATTGCTCTGGAGCAGTCTAGACTGGCTAGGCTGGACCCTCACCCCGAGCTGCTGC
CCGAACTGGGATCTCTGCCTCCCGTGAATACCGGAGGTGGCGGATCGGGAGACAAGAAGTA
CAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTAC
AAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGA
ACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCG
GACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTC
AGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGG
TGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGC
CTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGAC
AAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTT
CCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTG
GTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCA
AGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCT
GCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACC
CCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACA
CCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTC
CTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCG
AGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGA
CCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCT
TCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTT
CTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAG
CTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACC
AGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTG
AAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCC
CCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCAC
CCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGG
ATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGT
ACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCG
GAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACC
AACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCG
ACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGA
CCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTG
GAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGA
AAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACAC
CGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAA
GACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCC
ACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGA
CAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTG
CAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAAC
ATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGG
GAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAG
CACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACG
GCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGC
CGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGG
AGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATG
AAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACC
TGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCA
GCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAAC
ACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCA
AGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTA
CCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTAC
CCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGA
TCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACAT
CATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTG
ATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCG
TGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGG
CGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAG
AAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGC
TGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGC
TGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGC
CAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTC
GAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAAC
GAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCT
GAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTA
CCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCC
AACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGG
CCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTAC
TTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCC
TGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGG
CGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAA
GTCGGGCGGGGGTGGCTCAGACGCTAAGTCTCTGACCGCTTGGAGCAGAACACTGGTCACCT
TCAAGGACGTGTTCGTCGACTTCACAAGAGAGGAGTGGAAACTGCTGGACACCGCCCAGCA
GATCCTCTATAGAAACGTCATGCTGGAGAACTACAAGAATCTGGTGTCTCTGGGCTACCAGC
TGACCAAGCCCGACGTGATTCTGAGGCTGGAGAAGGGCGAGGAGCCTTGGCTGGTGGAGAG
AGAGATCCACCAAGAAACCCACCCCGACAGCGAAACCGCCTTCGAGATCAAGAGCAGCGTG
GGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCACCAAGAAGGCCGGTCAGGCCAAGAAG
AAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCT
GCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGG
TCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID
NO: 31) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 43 or 78. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 42 or 77 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having .. no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
G9A-dCas9-KRAB
MAPKKKRKVGIHGVPAASGGGGSGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVL
LFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYEN
VPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYD
KDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQG
TFICEYVGELISDAEAD VREDD SYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFML
HQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLD
PHPELLPELGS LPPVNTGGGGS GDKKYSIGLAIGTNSVGWAVITDEYKVPS KKFKVLGNTDRHSI
KKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEE
DKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDL
NPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFG
NLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSD
ILRVNTEITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS Q
EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKD
NREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQL
KEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREM
IEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIV
IEMARENQTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENTQLQNEKLYLYYLQNGRDMYV
DQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLL
NAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIRE
VKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFAT
VRKVLSMPQVNIVKKTEVQTGGFS KESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKR
MLASAGELQKGNELALPS KYVNFLYLAS HYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQIS EFS
KRVILADANLD KVLSAYNKHRD KPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYT STKEVL
DATLIHQSITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSDAKSLTAWSRTLVT
FKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREI
HQETHPDSETAFEIKSSVGGGGSGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 43) G9A-dCas9-KRAB without HA tag MAPKKKRKVGIHGVPAASGGGGSGS AAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVL
LFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYEN
VPIPCVNGVDGEPCPEDYKYISENCETS TMNIDRNITHLQHCTCVDDC S S SNCLCGQLSIRCWYD
KDGRLLQEFNKIEPPLIFECNQACS CWRNC KNRVV QSGIKVRLQLYRTAKMGWGVRALQTIPQG
TFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFML
HQDLRFPRIAFFS SRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLD
PHPELLPELGS LPPVNTGGGGS GD KKYSIGLAIGTNSVGWAVITDEYKVPS KKFKVLGNTDRHSI
KKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEE
DKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDL
NPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFG
NLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSD
ILRVNTEITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGASQ
EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKD
NREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQL
KEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREM
IEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIV
IEMARENQTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENTQLQNEKLYLYYLQNGRDMYV
DQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLL
NAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIRE
VKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFAT
VRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKR
MLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFS
KRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSDAKSLTAWSRTLVT
FKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREI
HQETHPDSETAFEIKSSVGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID NO: 78) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a first repressor domain comprising FOG1 e.g., a FOG1 domain and a second repressor domain comprising FOG1, e.g., a FOG1 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 32 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 32 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
FOG1-dCas9-FOG1 (MR-29434) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCTCGGGCGGGGGTGGCTCAAGCAG
AAGGAAGCAGAGCAACCCCAGACAAATCAAGAGATCTCTGGGCGACATGGAGGCCAGAGA
GGAAGTGCAGCTGGTGGGCGCCAGCCACATGGAGCAGAAGGCTACAGCCCCCGAGGCCCCC
AGCCCCGGAGGTGGCGGATCGGGAGACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCA
ACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGT
GCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGAC
AGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGC
GGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGA
CAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGG
CACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTA
CCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTACCTG
GCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGA
CAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAG
GAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCA
AGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTT
CGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGG
CCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCT
GGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCC
ATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCA
GCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCG
GCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCC
GGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGG
AGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGA
AGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGC
CATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGGGAGAAGATCGAG
AAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTT
CGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTTCGAGGAGGTGGTG
GACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGACAAGAACCTGC
CCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTACAACGA
GCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAG
CAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTGACCGTGAAGCAGC
TGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGA
GGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGATCATCAAGGACAAG
GACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTGCTGACCCTGACCC
TGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCCACCTGTTCGACGA
CAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTGAGCCGGAA
GCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTGAAATCC
GACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTCAAGGA
GGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAGCACATCGCCAAC
CTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTGGACGAGC
TGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAA
CCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCGGATCGAGGAGGG
CATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAACACCCAGCTGCAG
AACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTACGTGGACCAGGAGC
TGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAG
GACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCGGGGCAAGAGCGAC
AACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACG
CCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAG
CGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAG
CACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGA
TCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTT
CCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAAC
GCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACG
GCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAGATCGGCA
AGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACC
CTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGCGAGACCGGCGAGA
TCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGT
GAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCC
AAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGC
GGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCA
AGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGAGCAG
CTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGAC
CTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGC
TGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAA
CTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGGACAACGAGCAG
AAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCG
AGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCTGAGCGCCTACAA
CAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACCCTG
ACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAAGCGGTA
CACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTAC
GAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCA
CCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCTCAAGCAGAAGGA
AGCAGAGCAACCCCAGACAAATCAAGAGATCTCTGGGCGACATGGAGGCCAGAGAGGAAG
TGCAGCTGGTGGGCGCCAGCCACATGGAGCAGAAGGCTACAGCCCCCGAGGCCCCCAGCCC
CGGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCACCAAGAAGGCCGGTCAGGCCAAGAA
GAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGC
TGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTG
GTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID
NO: 32) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 44 or 79. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 44 or 79 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
FOG1-dCas9-FOG1 MAPKKKRKVGIHGVPAASGGGGSSRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPE
APS PGGGGS GD KKYSIGLAIGTNS VGWAVITDEYKVPSKKFKVLGNTDRHS IKKNLIGALLFD S G
ETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI
VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQ
LV QTYNQLFEENPINAS GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFK
S NFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILL SD ILRVNTEIT KAPLS
ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLL
YEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSV
EISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDD
KVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQ
KAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYD
VAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLT
KAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDF
RKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI
GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNI
VKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKL
KSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AGELQKG
NELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDK
VLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLY
ETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGS SRRKQSNPRQIKRSLGDMEAREEVQL
VGASHMEQKATAPEAPSPGGGGSGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 44) FOG1-dCas9-FOG1 without HA tag MAPKKKRKVGIHGVPAASGGGGSSRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPE
APS PGGGGS GD KKYSIGLAIGTNS VGWAVITDEYKVPSKKFKVLGNTDRHS IKKNLIGALLFD S G
ETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI
VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQ
LVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFK
S NFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILL SD ILRVNTEIT KAPLS
ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLL
YEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSV
EISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDD
KVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQ
KAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYD
VAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLT
KAERGGLSELDKAGFIKRQLVETRQITKHVAQILD SRMNTKYDENDKLIREVKVITLKS KLVS DF
RKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI
GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNI
VKKTEV QTGGFS KESILPKRNSDKLIARKKDWDPKKYGGFD S PTVAYSVLVVAKVEKGKSKKL
KSV KELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AGELQKG
NELALPS KYVNFLYLAS HYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQIS EFSKRVILADANLDK
VLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLY
ETRIDLS QLGGD S GGKRPAAT KKAGQAKKKKSGGGGS SRRKQSNPRQIKRS LGDMEAREEVQL
VGASHMEQKATAPEAPSPGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID NO: 79) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising DNMT, e.g., a DNMT3b domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 15 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 15 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT3b AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCT
CAGTGGACGTGCTGAGGAGAAGGAAGGACTGGAACGTGAGACTGCAAGCCTTCTTCACCAG
CGACACCGGACTGGAATACGAGGCCCCTAAGCTGTACCCCGCCATTCCCGCCGCTAGACGCA
GACCCATTAGAGTGCTCTCTCTGTTTGACGGAATCGCCACCGGCTATCTGGTGCTGAAAGAG
CTGGGCATCAAGGTGGGCAAATACGTGGCCAGCGAGGTGTGCGAGGAGTCTATCGCCGTCG
GAACCGTGAAACACGAGGGAAACATCAAATACGTGAACGACGTGAGGAACATCACCAAGA
AAAACATCGAGGAGTGGGGCCCCTTCGATCTCGTGATCGGCGGCAGCCCTTGCAACGATCTC
TCCAATGTGAACCCCGCCAGAAAGGGACTGTACGAGGGCACCGGCAGACTGTTCTTCGAGTT
CTACCATCTGCTGAACTACAGCAGACCCAAGGAGGGCGACGACAGACCCTTCTTCTGGATGT
TCGAAAACGTGGTCGCCATGAAGGTGGGCGATAAGAGGGACATCAGCAGATTTCTGGAGTG
TAACCCCGTCATGATCGATGCCATTAAGGTCAGCGCCGCCCACAGAGCTAGGTACTTCTGGG
GAAATCTGCCCGGCATGAATAGACCCGTGATCGCTTCCAAGAACGACAAGCTGGAGCTGCA
AGACTGTCTGGAGTACAATAGAATCGCTAAGCTCAAGAAGGTCCAGACCATCACCACAAAA
TCCAACAGCATCAAGCAAGGCAAGAACCAGCTGTTCCCCGTGGTCATGAACGGCAAGGAGG
ATGTGCTCTGGTGCACCGAGCTGGAGAGGATCTTTGGCTTCCCCGTGCACTATACCGATGTG
TCCAACATGGGAAGAGGCGCTAGACAGAAACTGCTGGGAAGAAGCTGGAGCGTGCCCGTGA
TTAGACACCTCTTCGCCCCTCTGAAGGATTACTTCGCTTGCGAGGGAGGTGGCGGATCGGGA
AAGCGGCCCGCCGCCACCAAGAAGGCCGGTCAGGCCAAGAAGAAGAAGGGCAGCTACCCC
TACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCC
TTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAG
TAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 15) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 16 or 37. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 16 or 37 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT3b MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNL SDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSVDVLRRRKDWNVRLQAFFTS
DTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKH
EGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSR
PKEGDDRPFFWMFENVVAMKVGDKRDISRFLECNPVMIDAIKVS AAHRARYFWGNLPGMNRPV
IAS KND KLELQDCLEYNRIAKLKKV QTITT KS NSIKQGKNQLFPVVMNGKEDVLWCTELERIFGF
PVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACEGGGGSGKRPAATKKAGQAKK
KKGSYPYDVPDYA (SEQ ID NO: 16) dCas9-DNMT3b without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDIN
RLSDYDVAAIVPQSFLKDD SIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLIT Q
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGD S GGKRPAAT KKAGQAKKKKS GGGGSVDVLRRRKDWNVRLQAFFTS
DTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKH
EGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSR
PKEGDDRPFFWMFENVVAMKVGDKRDISRFLECNPVMIDAIKVSAAHRARYFWGNLPGMNRPV
IASKNDKLELQDCLEYNRIAKLKKVQTITTKSNSIKQGKNQLFPVVMNGKEDVLWCTELERIFGF
PVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACEGGGGSGKRPAATKKAGQAKK
KKGS (SEQ ID NO: 37) Functional Characteristics An expression repression system of the present disclosure can be used to decrease expression of a target gene in a cell. In some embodiments, decreasing expression comprises decreasing the level of RNA, e.g., mRNA, encoded by the target gene. In some embodiments, decreasing expression comprises decreasing the level of a protein encoded by the target gene. In some embodiments, decreasing expression comprises both decreasing the level of mRNA and protein encoded by the target gene. In some embodiments, the expression of a target gene in a cell contacted by or comprising the expression repression system is at least 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.55x, 1.6x, 1.65x, 1.7x, 1.75x, 1.8x, 1.85x, 1.9x, 1.95x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 30x, 40x, 50x, 60x, 70x, 80x, 90x, or 100x lower than the level of expression of the target gene in a cell not contacted by or comprising the expression repression system. Expression of a target gene may be assayed by methods known to those of skill in the art, including RT-PCR, ELISA, Western blot, and the methods of Examples 2-4.
An expression repression system of the present disclosure can be used to decrease expression of a target gene in a cell for a time period. In some embodiments, the expression of a target gene in a cell contacted by or comprising the expression repression system is appreciably decreased for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6, 7, 10, or 14 days, or at least 1, 2, 3, 4, or 5 weeks, or at least 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely). Optionally, the expression of a target gene in a cell contacted by or comprising the expression repression system is appreciably decreased for no more than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 years.
In some embodiments, the expression of a target in a cell contacted by or comprising the .. expression repressor or the system is appreciably decreased for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions. An expression repressor or a system of the present disclosure can be used to methylate CpG
nucleotides in a target region. In some embodiments, the methylation persists for at least 1 days, at least 2 days, at least 5 days, at least 7 days, at least 10 days, at least 15 days, or at least 22 days post-treatment with an expression repressor or an expression repression system disclosed herein.
An expression repression system may comprise a plurality of expression repressors, where each expression repressor comprises a repressor domain with a different functionality than the repressor domain of another expression repressor. For example, an expression repression system may comprise two expression repressors, where the first expression repressor comprises a first repressor domain comprising histone deacetylase functionality and the second expression repressor comprises a second repressor domain comprising DNA methyltransferase functionality. In some embodiments, an expression repression system comprises expression repressors comprising a combination of repressor domains whose functionalities are complementary to one another with regard to inhibiting expression of a target gene, e.g., where the functionalities together enable inhibition of expression and, optionally, do not inhibit or negligibly inhibit expression when present individually. In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each expression repressor comprises a repressor domain that complements the repressor domains of each other expression repressor, e.g., each repressor domain decreases expression of a target gene.
In some embodiments, an expression repression system comprises expression repressors .. comprising a combination of repressor domains whose functionalities synergize with one another with regard to inhibiting expression of a target gene. Without wishing to be bound by theory, it is thought that epigenetic modifications to a genomic locus are cumulative, in that multiple repressive epigenetic markers (e.g., multiple different types of epigenetic markers and/or more extensive marking of a given type) individually together reduce expression more effectively than individual modifications alone (e.g., producing a greater decrease in expression and/or a longer-lasting decrease in expression). In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each expression repressor comprises a repressor domain that synergizes with the repressor domains of each other expression repressor, e.g., each repressor domain decreases expression of a target gene. In some embodiments, an expression repression system (comprising a plurality of expression repressors comprising a plurality of different repressor domains which synergize with one another) is more effective at inhibiting expression of a target gene than an individual expression repressor, e.g., and its single repressor domain. In some embodiments, an expression repression system is at least 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.55x, 1.6x, 1.65x, 1.7x, 1.75x, 1.8x, 1.85x, 1.9x, 1.95x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 30x, 40x, 50x, 60x, 70x, 80x, 90x, or 100x as effective at inhibiting expression of a target gene than an individual expression repressor. The level of an epigenetic marker may be assayed by methods known to those of skill in the art, including whole genome bisulfite sequencing, reduced representation bisulfite sequencing, bisulfite amplicon sequencing, methylation arrays, pyrosequencing, ChIP-seq, or ChIP-qPCR.
Combinations of Repressors In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor domain wherein the first repressor domain and second repressor domain are different from one another. In some embodiments, the first repressor domain is or comprises a first epigenetic modifying moiety (e.g., that increases or decreases a first epigenetic marker) or functional fragment thereof and the second repressor domain is or comprises a second epigenetic modifying moiety (e.g., that increases or decreases a second epigenetic marker) or functional fragment thereof. In some embodiments, the first repressor domain is or comprises a histone methyltransferase or functional fragment thereof and the second repressor domain is or comprises a DNA methyltransferase or functional fragment thereof. In some embodiments, the first repressor domain is or comprises a histone deacetylase or functional fragment thereof and the second repressor domain is or comprises a DNA
methyltransferase or functional fragment thereof. In some embodiments, the first repressor domain is or comprises a histone deacetylase or functional fragment thereof and the second repressor domain is or comprises a histone methyltransferase or functional fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB (e.g., a KRAB domain), a SET domain (e.g., the SET domain of SETDB1 EZH2, G9A, or SUV39H1), histone demethylase LSD1, FOG1 (e.g., the N-terminal residues of FOG1), HDAC8, MQ1, DNMT1, DNMT3a/31, or KAP1, or a functional fragment of any thereof, and the second repressor domain is or comprises KRAB (e.g., a KRAB domain), a SET domain (e.g., the SET domain of SETDB1 EZH2, G9A, or SUV39H1), histone demethylase LSD1, FOG1 (e.g., the N-terminal residues of FOG1), DNMT3A (e.g., human DNMT3A), DNMT3B, DNMT3L, DNMT3A/3L complex, or bacterial MQ1, or a functional fragment of any thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises DNMT3A or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises DNMT3B or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises DNMT3L or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises DNMT3A/3L
complex or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises a SET domain or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises LSD1 or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises FOG1 or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KAP1 or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant .. or fragment thereof.
In some embodiments, an expression repression system comprises a first expression repressor, wherein the first expression repressor is an expression repressor chosen from dCas9-MQ1, dCas9-DNMT1, dCas9-DNMT3a/31, G9A-dCas9, dCas9-HDAC8, dCas9-LSD1, EZH2-dCas9, EZH2-dCas9-KRAB, G9a-dCas9-KRAB, and Fogl-dCas9-Fogl, and a second expression repressor, wherein the second expression repressor is an expression repressor chosen from dCas9-MQ1, dCas9-DNMT1, dCas9-DNMT3a/31, G9A-dCas9, dCas9-HDAC8, dCas9-LSD1, EZH2-dCas9, EZH2-dCas9-KRAB, G9a-dCas9-KRAB, and Fogl-dCas9-Fogl.
In some embodiments, the expression repression system is encoded by a first nucleic acid encoding the first expression repressor, wherein expression is driven by a first promoter or IRES, and a second nucleic acid encoding the second expression repressor, wherein expression is driven by a second promoter or IRES.
Target Sites Expression repression systems disclosed herein are useful for modulating, e.g., decreasing, expression of a target gene in cell, e.g., in a subject or patient. A target gene may be any gene known to those of skill in the art. In some embodiments, a target gene is associated with a disease or condition in a subject, e.g., a mammal, e.g., a human, bovine, horse, sheep, chicken, rat, mouse, cat, or dog. A target gene may include coding sequences, e.g., exons, and/or non-coding sequences, e.g., introns, 3'UTR, or 5'UTR. In some embodiments, a target gene is operably linked to a transcription control element.
A DNA-targeting moiety suitable for use in an expression repressor of an expression repression system described herein may bind, e.g., specifically bind, to any site within a target gene, transcription control element operably linked to a target gene, or anchor sequence (e.g., an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene (e.g., an anchor sequence-mediated conjunction is operably linked to a target gene if disruption of the conjunction alters expression of the target gene)).
In some embodiments, a DNA-targeting moiety binds to a target gene. In some embodiments, a DNA-targeting moiety binds to a site within an exon of a target gene. In some embodiments, a DNA-targeting moiety binds to a site within an intron of a target gene. In some embodiments, a DNA-targeting moiety binds to a site at the boundary of an exon and an intron, e.g., a splice site, of a target gene. In some embodiments, a DNA-targeting moiety binds to a site within the 5'UTR of a target gene. In some embodiments, a DNA-targeting moiety binds to a site within the 3'UTR of a target gene. Target genes include, but are not limited to, the gene encoding13-2-microglobulin, the gene encoding MYC, the gene encoding HSPA1B, the gene encoding GATA1, the gene encoding APOB, the gene encoding FOXP3, the gene encoding CXCL1, the gene encoding CXCL2, the gene encoding CXCL3, the gene encoding CXCL4, the gene encoding CXCL5, the gene encoding CXCL6, the gene encoding CXCL7, and the gene encoding CXCL8.
In some embodiments, a DNA-targeting moiety binds to a transcription control element operably linked to a target gene, e.g., a promoter or enhancer. In some embodiments, a DNA-targeting moiety binds to a portion of or a site within a promoter operably linked to a target gene. In some embodiments, a DNA-targeting moiety binds to the transcription start site of a target gene.
In some embodiments, a DNA-targeting moiety binds to a portion of or a site within an enhancer operably linked to a target gene. A
promoter is, typically, a sequence element that initiates transcription of an associated gene. Promoters are typically near the 5' end of a gene, not far from its transcription start site. As those of ordinary skill are aware, transcription of protein-coding genes in eukaryotic cells is typically initiated by binding of general transcription factors (e.g., TFIID, TFIIE, TFIIH, etc.) and Mediator to core promoter sequences as a preinitiation complex that directs RNA polymerase II to the transcription start site, and in many instances remains bound to the core promoter sequences even after RNA polymerase escapes and elongation of the primary transcript is initiated. In some embodiments, a promoter includes a sequence element such as TATA, Inr, DPE, or BRE, but those skilled in the art are well aware that such sequences are not necessarily required to define a promoter. Those skilled in the art are familiar with a variety of positive .. (e.g., enhancers) or negative (e.g., repressors or silencers) transcription control elements that are associated with genes. In some embodiments, a transcription control element is a transcription factor binding site. Typically, when a cognate regulatory protein is bound to such a transcription control element, transcription from the associated gene(s) is altered (e.g., increased or decreased).
In some embodiments, a DNA-targeting moiety binds to an anchor sequence, e.g., an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene (e.g., an anchor sequence-mediated conjunction is operably linked to a target gene if disruption of the conjunction alters expression of the target gene). In some embodiments, a DNA-targeting moiety binds to or proximal to an anchor sequence of an anchor sequence mediated conjunction (ASMC) comprising a target plurality of genes, e.g., human CXCL1-8. In general, an anchor sequence is a genomic sequence element to which a genomic complex component binds specifically. In some embodiments, binding of a genomic complex component to an anchor sequence nucleates complex formation, e.g., anchor sequence-mediated conjunction formation. Each anchor sequence-mediated conjunction comprises one or more anchor sequences, e.g., a plurality. In some embodiments, an anchor sequence-mediated conjunction can be disrupted to alter, e.g., inhibit, expression of a target gene. Such disruptions may modulate gene expression by, e.g., changing topological structure of DNA, e.g., by modulating the ability of a target gene to interact with a transcription control element (e.g., enhancing and silencing/repressive sequences).
A DNA-targeting moiety suitable for use in an expression repressor of an expression repression system described herein may bind, e.g., specifically bind, to a site that is proximal to a target gene (e.g., an exon, intron, or splice site within the target gene), proximal to a transcription control element operably linked to the target gene, or proximal to an anchor sequence, e.g., an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene (e.g., an anchor sequence-mediated conjunction is operably linked to a target gene if disruption of the conjunction alters expression of the target gene). As used herein, proximal refers to a closeness of two sites, e.g., nucleic acid sites, such that binding of an expression repressor at the first site and/or modification of the first site by an expression repressor will produce the same or substantially the same effect as binding and/or modification of the other site. For example, a DNA-targeting moiety may bind to a first site that is proximal to an enhancer (the second site), and the repressor domain associated with said DNA-targeting moiety may epigenetically modify the first site such that the enhancer's effect on expression of a target gene is modified, substantially the same as if the second site (the enhancer sequence) had been bound and/or modified. In some embodiments, a site proximal to a target gene (e.g., an exon, intron, or splice site within the target gene), proximal to a transcription control element operably linked to the target gene, or proximal to an anchor sequence is less than 5000, 4000, 3000, 2000, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50, or 25 base pairs from the target gene (e.g., an exon, intron, or splice site within the target gene), transcription control element, or anchor sequence (and optionally at least 20, 25, 50, 100, 200, or 300 base pairs from the target gene (e.g., an exon, intron, or splice site within the target gene), transcription control element, or anchor sequence).
A DNA-targeting moiety suitable for use in an expression repressor of an expression repression system described herein may bind, e.g., specifically bind, to a site comprising at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides or base pairs (and optionally no more 50, 49, 48, 47, 46, 45, 44, 43, 42, 41, 40, 39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, or 10 nucleotides or base pairs). In some embodiments, a DNA-targeting moiety binds to a site comprising 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides or base pairs.
Expression repression systems of the present disclosure may comprise two or more expression repressors. In some embodiments, the expression repressors of an expression repression system each comprise a different DNA-targeting moiety.
In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds a target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site), and second expression repressor comprising a DNA-targeting moiety that binds the target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site). In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds a target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site), and second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to the target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site). In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to the target gene or associated with an anchor sequence-mediated conjunction operably linked to the target gene.
In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to a first site, e.g., in a promoter operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a second site, e.g., in the promoter operably linked to a target gene. The first site and second site may be different and non-overlapping sites, e.g., the first site and second site do not share any sequence in common. The first site and second site may be different but overlapping sites, e.g., the first site and second site comprise different sequences but share some sequence in common.
In some embodiments, a DNA-targeting moiety binds to a sequence selected from SEQ ID NOs:
1-21 or a sequence with no more than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 alteration relative thereto.
ACGGCGGGCCACCAAGGAGA (SEQ ID NO: 6) CAGCGCCGCGCCTTTGGGAC (SEQ ID NO: 7) CCCTTTCGGCGGGGAGCAGGG (SEQ ID NO: 8) GCTGCGCTGGGGGAGCCAGAG (SEQ ID NO: 9) AGCGCCCCTCCACGCGTTCAC (SEQ ID NO: 10) In some embodiments, the first DNA-targeting moiety binds to a sequence comprising any one of SEQ ID NOS: 1-21 and the second DNA-targeting moiety binds to a sequence comprising any one of SEQ ID NOS: 1-21, wherein the first and the second DNA-targeting moiety binds to the same sequence.
In some embodiments, the first DNA-targeting moiety binds to a sequence comprising any one of SEQ ID
NOS: 1-21 and the second DNA-targeting moiety binds to a sequence comprising any one of SEQ ID
NOS: 1-21 wherein the first and the second targeting moiety binds to different sequences.
In some embodiments, an expression repressor binds a genomic locus having a sequence set forth herein, e.g., any one of SEQ ID NOS: 1-21. It is understood that, in many cases, the genomic locus being bound comprises double stranded DNA, and this locus can be described by giving the sequence of its sense strand or its antisense strand. Thus, a gRNA having a given spacer sequence may cause expression repressor to bind to a particular genomic locus, wherein one strand of the genomic locus has a sequence similar or identical to the spacer sequence, and the other strand of the genomic locus has the complementary sequence. Typically, gRNA binding to the genomic locus will involve some unwinding of the genomic locus and pairing of the gRNA spacer with the strand to which the spacer is complementary.
In some embodiments, an anchor sequence comprises a nucleating polypeptide binding motif, e.g., a CTCF-binding motif:
N(T/C/G)N(G/A/T)CC(A/T/G)(C/G)(C/T/A)AG(G/A)(G/T)GG(C/A/T)(G/A)(C/G)(C/T/A)(G/A
/C) (SEQ ID NO: 81), where N is any nucleotide.
A CTCF-binding motif may also be in an opposite orientation, e.g., (G/A/C)(C/T/A)(C/G)(G/A)(C/A/T)GG(G/T)(G/A)GA(C/T/A)(C/G)(A/T/G)CC(G/A/T)N(T/C/
G)N
(SEQ ID NO: 82). where N is any nucleotide In some embodiments, an anchor sequence comprises SEQ ID NO: 81 or SEQ ID NO:
82 or a sequence at least 75%, at least 80%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identical to either SEQ ID NO: 81 or SEQ ID NO: 82.
In some embodiments, an anchor sequence comprises a nucleating polypeptide binding motif, e.g., a YY1-binding motif: CCGCCATNTT (SEQ ID NO: 83), where N is any nucleotide.
A YY1-binding motif may also be in an opposite orientation, e.g., AANATGGCGG
(SEQ ID
NO: 84), where N is any nucleotide.
A targeting moiety suitable for use in an expression repressor of an expression repression system described herein may bind, e.g., specifically bind, to a site comprising at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides or base pairs (and optionally no more 50, 49, 48, 47, 46, 45, 44, 43, 42, 41, 40, 39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, or 10 nucleotides or base pairs). In some embodiments, a DNA-targeting moiety binds to a site comprising 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides or base pairs.
Expression repression systems of the present disclosure may comprise two or more expression repressors. In some embodiments, the expression repressors of an expression repression system each comprise a different targeting moiety.
In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds a target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site), and second expression repressor comprising a DNA-targeting moiety that binds the target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site). In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds a target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site), and second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene, e or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene,. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene, or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to the target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site). In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene, or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to the target gene, or associated with an anchor sequence-mediated conjunction operably linked to the target gene.
Other Compositions Nucleic acids and Vectors The present disclosure is further directed, in part, to nucleic acids encoding expression repressors or expression repression systems described herein. In some embodiments, an expression repression system may be provided via a composition comprising a nucleic acid encoding the expression repression system, e.g., expression repressor(s) of the expression repression system, wherein the nucleic acid is associated with sufficient other sequences to achieve expression of the expression repression system, e.g., expression repressor(s) of the expression repression system, in a system of interest (e.g., in a particular cell, tissue, organism, etc.).
In some particular embodiments, the present disclosure provides compositions of nucleic acids that encode an expression repression system, one or more expression repressors, or polypeptide portion thereof. In some such embodiments, provided nucleic acids may be or include DNA, RNA, or any other nucleic acid moiety or entity as described herein, and may be prepared by any technology described herein or otherwise available in the art (e.g., synthesis, cloning, amplification, in vitro or in vivo transcription, etc.). In some embodiments, provided nucleic acids that encode an expression repression system, one or more expression repressors, or polypeptide portion thereof may be operationally associated with one or more replication, integration, and/or expression signals appropriate and/or sufficient to achieve integration, replication, and/or expression of the provided nucleic acid in a system of interest (e.g., in a particular cell, tissue, organism, etc.). In some embodiments, a composition for delivering an expression repression system described herein is or comprises a vector, e.g., a viral vector, comprising .. one or more nucleic acids encoding one or more components of an expression repression system, e.g., expression repressor(s) of the expression repression system as described herein.
In some embodiments, a composition for delivering an expression repressor or an expression repression system described herein is or comprises RNA, e.g., mRNA, comprising one or more nucleic acids encoding one or more components of an expression repressor or an expression repression system, e.g., expression repressor(s) of the expression repression system as described herein.
Nucleic acids as described herein or nucleic acids encoding a protein described herein, may be incorporated into a vector. Vectors, including those derived from retroviruses such as lentivirus, are .. suitable tools to achieve long-term gene transfer since they allow long-term, stable integration of a transgene and its propagation in daughter cells. Examples of vectors include expression vectors, replication vectors, probe generation vectors, and sequencing vectors. An expression vector may be provided to a cell in the form of a viral vector. Viral vector technology is well known in the art and described in a variety of virology and molecular biology manuals. Viruses, which are useful as vectors .. include, but are not limited to, retroviruses, adenoviruses, adeno-associated viruses, herpes viruses, and lentiviruses. In general, a suitable vector contains an origin of replication functional in at least one organism, a promoter sequence, convenient restriction endonuclease sites, and one or more selectable markers.
Expression of natural or synthetic nucleic acids is typically achieved by operably linking a .. nucleic acid encoding the gene of interest to a promoter and incorporating the construct into an expression vector. Vectors can be suitable for replication and integration in eukaryotes.
Typical cloning vectors contain transcription and translation terminators, initiation sequences, and promoters useful for expression of the desired nucleic acid sequence.
Additional promoter elements, e.g., enhancing sequences, may regulate frequency of .. transcriptional initiation. Typically, these sequences are located in a region 30-110 bp upstream of a transcription start site, although a number of promoters have recently been shown to contain functional elements downstream of transcription start sites as well. Spacing between promoter elements frequently is flexible, so that promoter function is preserved when elements are inverted or moved relative to one another. In a thymidine kinase (tk) promoter, spacing between promoter elements can be increased to 50 .. bp apart before activity begins to decline. Depending on the promoter, it appears that individual elements can function either cooperatively or independently to activate transcription.
One example of a suitable promoter is the immediate early cytomegalovirus (CMV) promoter sequence. This promoter sequence is a strong constitutive promoter sequence capable of driving high levels of expression of any polynucleotide sequence operatively linked thereto. In some embodiments of .. a suitable promoter is Elongation Growth Factor-1a (EF-1a). However, other constitutive promoter sequences may also be used, including, but not limited to the simian virus 40 (5V40) early promoter, mouse mammary tumor virus (MMTV), human immunodeficiency virus (HIV) long terminal repeat (LTR) promoter, MoMuLV promoter, an avian leukemia virus promoter, an Epstein-Barr virus immediate early promoter, a Rous sarcoma virus promoter, as well as human gene promoters such as, but not limited to, an actin promoter, a myosin promoter, a hemoglobin promoter, and a creatine kinase promoter.
The present disclosure should not interpreted to be limited to use of any particular promoter or category of promoters (e.g. constitutive promoters). For example, in some embodiments, inducible promoters are contemplated as part of the present disclosure. In some embodiments, use of an inducible promoter provides a molecular switch capable of turning on expression of a polynucleotide sequence to which it is operatively linked, when such expression is desired. In some embodiments, use of an inducible promoter provides a molecular switch capable of turning off expression when expression is not desired. Examples of inducible promoters include, but are not limited to a metallothionine promoter, a glucocorticoid promoter, a progesterone promoter, and a tetracycline promoter.
In some embodiments, an expression vector to be introduced can also contain either a selectable marker gene or a reporter gene or both to facilitate identification and selection of expressing cells from the population of cells sought to be transfected or infected through viral vectors. In some aspects, a selectable marker may be carried on a separate piece of DNA and used in a co-transfection procedure.
Both selectable markers and reporter genes may be flanked with appropriate transcriptional control sequences to enable expression in the host cells. Useful selectable markers may include, for example, antibiotic-resistance genes, such as neo, etc.
In some embodiments, reporter genes may be used for identifying potentially transfected cells and/or for evaluating the functionality of transcriptional control sequences.
In general, a reporter gene is a gene that is not present in or expressed by a recipient source (of a reporter gene) and that encodes a polypeptide whose expression is manifested by some easily detectable property, e.g., enzymatic activity or visualizable fluorescence. Expression of a reporter gene is assayed at a suitable time after the DNA
has been introduced into the recipient cells. Suitable reporter genes may include genes encoding luciferase, beta-galactosidase, chloramphenicol acetyl transferase, secreted alkaline phosphatase, or the green fluorescent protein gene (e.g., Ui-Tei et al., 2000 FEBS Letters 479: 79-82). Suitable expression systems are well known and may be prepared using known techniques or obtained commercially. In general, a construct with a minimal 5' flanking region that shows highest level of expression of reporter gene is identified as a promoter. Such promoter regions may be linked to a reporter gene and used to evaluate agents for ability to modulate promoter-driven transcription.
Cells The present disclosure is further directed, in part, to cells comprising an expression repressor or an expression repression system described herein. Any cell, e.g., cell line, e.g., a cell line suitable for expression of a recombinant polypeptide, known to one of skill in the art is suitable to comprise an expression repressor or an expression repression system described herein. In some embodiments, a cell, e.g., cell line, may be used to express an expression repressor or an expression repression system, e.g., expression repressor(s), described herein. In some embodiments, a cell, e.g., cell line, may be used to express or amplify a nucleic acid, e.g., a vector, encoding an expression repressor or an expression repression system, e.g., expression repressor(s), described herein. In some embodiments, a cell comprises a nucleic acid encoding an expression repressor or an expression repression system, e.g., expression repressor(s), described herein.
In some embodiments, a cell comprises a first nucleic acid encoding a first component of an expression repressor or an expression repression system, e.g., a first expression repressor, and a second nucleic acid encoding a second component of the expression repression system, e.g., a second expression repressor. In some embodiments, wherein a cell comprises nucleic acid encoding an expression repression system comprising two or more expression repressors, the sequences encoding each expression repressor are disposed on separate nucleic acid molecules, e.g., on different vectors, e.g., a first vector encoding a first expression repressor and a second vector encoding a second expression repressor. In some embodiments, the sequences encoding each expression repressor are disposed on the same nucleic acid molecule, e.g., on the same vector. In some embodiments, some or all of the nucleic acid encoding the expression repression system is integrated into the genomic DNA of the cell.
In some embodiments, the nucleic acid encoding a first expression repressor of an expression repression system is integrated into the genomic DNA of a cell, and the nucleic acid encoding a second expression repressor of an expression repression system is not integrated into the genomic DNA of a cell (e.g., is situated on a vector). In some embodiments, the nucleic acid(s) encoding a first and a second expression repressor of an expression repression system are integrated into the genomic DNA of a cell, e.g., at the same (e.g., adjacent or colocalized) or different sites in the genomic DNA.
Examples of cells that may comprise and/or express an expression repression system or expression repressor described herein include, but are not limited to, hepatocytes, neuronal cells, endothelial cells, myocytes, and lymphocytes.
The present disclosure is further directed, in part, to a cell made by a method or process described herein. In some embodiments, the disclosure provides a cell produced by:
providing an expression repression system described herein, providing the cell, and contacting the cell with the expression repression system (or a nucleic acid encoding the expression repression system, or a composition comprising said expression repression system or nucleic acid). Without wishing to be bound by theory, a cell contacted with an expression repression system described herein may exhibit: a decrease in expression of a target gene and/or a modification of epigenetic markers associated with the target gene, a transcription control element operably linked to the target gene, or an anchor sequence proximal to the target gene or associated with an anchor sequence-mediated conjunction operably linked to the target gene compared to a similar cell that has not been contacted by the expression repression system. In some embodiments, a cell exhibiting said decrease in expression of a target gene and/or modification of epigenetic markers does not comprise the expression repression system. The decrease in expression and/or modification of epigenetic markers may persist, e.g., for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6,7, 10, or 14 days, or at least 1,2, 3, 4, or 5 weeks, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely) after contact with the expression repression system. In some embodiments, a cell previously contacted by an expression repression system retains the decrease in expression and/or modification of epigenetic markers after the expression repression system is no longer present in the cell, e.g., for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6, 7, 10, or 14 days, or at least 1, 2, 3, 4, or 5 weeks, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely) after the expression repression system is no longer present in the cell.
Methods of Making Expression Repression Systems and/or Expression Repressors In some embodiments, an expression repressor comprises or is a protein and may thus be produced by methods of making proteins. In some embodiments, an expression repression system, e.g., the expression repressor(s) of an expression repression system, comprise one or more proteins and may thus be produced by methods of making proteins. As will be appreciated by one of skill, methods of making proteins or polypeptides (which may be included in modulating agents as described herein) are routine in the art. See, in general, Smales & James (Eds.), Therapeutic Proteins: Methods and Protocols (Methods in Molecular Biology), Humana Press (2005); and Crommelin, Sindelar &
Meibohm (Eds.), Pharmaceutical Biotechnology: Fundamentals and Applications, Springer (2013).
A protein or polypeptide of compositions of the present disclosure can be biochemically synthesized by employing standard solid phase techniques. Such methods include exclusive solid phase synthesis, partial solid phase synthesis methods, fragment condensation, classical solution synthesis.
These methods can be used when a peptide is relatively short (e.g., 10 kDa) and/or when it cannot be produced by recombinant techniques (i.e., not encoded by a nucleic acid sequence) and therefore involves different chemistry.
Solid phase synthesis procedures are well known in the art and further described by John Morrow Stewart and Janis Dillaha Young, Solid Phase Peptide Syntheses, 2nd Ed., Pierce Chemical Company, 1984; and Coin, I., et al., Nature Protocols, 2:3247-3256, 2007.
For longer peptides, recombinant methods may be used. Methods of making a recombinant therapeutic polypeptide are routine in the art. See, in general, Smales &
James (Eds.), Therapeutic Proteins: Methods and Protocols (Methods in Molecular Biology), Humana Press (2005); and Crommelin, .. Sindelar & Meibohm (Eds.), Pharmaceutical Biotechnology: Fundamentals and Applications, Springer (2013).
Exemplary methods for producing a therapeutic pharmaceutical protein or polypeptide involve expression in mammalian cells, although recombinant proteins can also be produced using insect cells, yeast, bacteria, or other cells under control of appropriate promoters.
Mammalian expression vectors may comprise non transcribed elements such as an origin of replication, a suitable promoter, and other 5' or 3' flanking non transcribed sequences, and 5' or 3' non-translated sequences such as necessary ribosome binding sites, a polyadenylation site, splice donor and acceptor sites, and termination sequences. DNA
sequences derived from the 5V40 viral genome, for example, 5V40 origin, early promoter, splice, and polyadenylation sites may be used to provide other genetic elements required for expression of a heterologous DNA sequence. Appropriate cloning and expression vectors for use with bacterial, fungal, yeast, and mammalian cellular hosts are described in Green & Sambrook, Molecular Cloning: A
Laboratory Manual (Fourth Edition), Cold Spring Harbor Laboratory Press (2012).
In cases where large amounts of the protein or polypeptide are desired, it can be generated using techniques such as described by Brian Bray, Nature Reviews Drug Discovery, 2:587-593, 2003; and Weissbach & Weissbach, 1988, Methods for Plant Molecular Biology, Academic Press, NY, Section VIII, pp 421-463.
Various mammalian cell culture systems can be employed to express and manufacture recombinant protein. Examples of mammalian expression systems include CHO
cells, COS cells, HeLA
and BHK cell lines. Processes of host cell culture for production of protein therapeutics are described in Zhou and Kantardjieff (Eds.), Mammalian Cell Cultures for Biologics Manufacturing (Advances in Biochemical Engineering/Biotechnology), Springer (2014). Compositions described herein may include a vector, such as a viral vector, e.g., a lentiviral vector, encoding a recombinant protein. In some embodiments, a vector, e.g., a viral vector, may comprise a nucleic acid encoding a recombinant protein.
Compositions described herein may include a lipid nanoparticle encapsulating a vector, such as a viral vector, e.g., a lentiviral vector, encoding a recombinant protein. In some embodiments, a lipid nanoparticle encapsulating a vector, e.g., a viral vector, may comprise a nucleic acid encoding a recombinant protein.
Proteins comprise one or more amino acids. Amino acids include any compound and/or substance that can be incorporated into a polypeptide chain, e.g., through formation of one or more peptide bonds.
In some embodiments, an amino acid has the general structure H2N¨C(H)(R)¨COOH.
In some embodiments, an amino acid is a naturally occurring amino acid. In some embodiments, an amino acid is a non-natural amino acid; in some embodiments, an amino acid is a D-amino acid; in some embodiments, an amino acid is an L-amino acid. "Standard amino acid" refers to any of the twenty standard L-amino acids commonly found in naturally occurring peptides. "Nonstandard amino acid"
refers to any amino acid, other than the standard amino acids, regardless of whether it is prepared synthetically or obtained from a natural source. In some embodiments, an amino acid, including a carboxy-and/or amino-terminal amino acid in a polypeptide, can contain a structural modification as compared with the general structure above. For example, in some embodiments, an amino acid may be modified by methylation, amidation, acetylation, pegylation, glycosylation, phosphorylation, and/or substitution (e.g., of the amino group, the carboxylic acid group, one or more protons, and/or the hydroxyl group) as compared with the general structure. In some embodiments, such modification may, for example, alter the circulating half-life of a polypeptide containing the modified amino acid as compared with one containing an otherwise identical unmodified amino acid. In some embodiments, such modification does not significantly alter a relevant activity of a polypeptide containing the modified amino acid, as compared with one containing an otherwise identical unmodified amino acid. As will be clear from context, in some embodiments, the term "amino acid" may be used to refer to a free amino acid; in some embodiments it may be used to refer to an amino acid residue of a polypeptide.
Purification of protein therapeutics is described in Franks, Protein Biotechnology: Isolation, Characterization, and Stabilization, Humana Press (2013); and in Cutler, Protein Purification Protocols (Methods in Molecular Biology), Humana Press (2010). Formulation of protein therapeutics is described in Meyer (Ed.), Therapeutic Protein Drug Products: Practical Approaches to formulation in the Laboratory, Manufacturing, and the Clinic, Woodhead Publishing Series (2012).
Pharmaceutical Compositions, Formulation, Delivery, and Administration The present disclosure is further directed, in part, to pharmaceutical compositions comprising an expression repressor or an expression repression system, e.g., expression repressor(s), described herein, to pharmaceutical compositions comprising nucleic acids encoding the expression repressor or the expression repression system, e.g., expression repressor(s), described herein, and/or to and/or compositions that deliver an expression repressor or an expression repression system, e.g., expression repressor(s), described herein to a cell, tissue, organ, and/or subject.
As used herein, the term "pharmaceutical composition" refers to an active agent (e.g., an expression repressor or nucleic acids of the expression receptor, e.g., an expression repression system, e.g., expression repressor(s) of an expression repression system, or nucleic acid encoding the same), formulated together with one or more pharmaceutically acceptable carriers (e.g., pharmaceutically acceptable carriers known to those of skill in the art). In some embodiments, active agent is present in unit dose amount appropriate for administration in a therapeutic regimen that shows a statistically significant probability of achieving a predetermined therapeutic effect when administered to a relevant population. In some embodiments, a pharmaceutical composition comprising an expression repressor of the present disclosure comprises an expression repressor or nucleic acid(s) encoding the same. In some embodiments, a pharmaceutical composition comprising an expression repression system of the present disclosure comprises or each of the expression repressors of the expression repression system or nucleic acid(s) encoding the same (e.g., if an expression repression system comprises a first expression repressor and a second expression repressor, the pharmaceutical composition comprises the first and second expression repressor). In some embodiments, a pharmaceutical composition comprises less than all of the expression repressors of an expression repression system comprising a plurality of expression repressors.
For example, an expression repression system may comprise a first expression repressor and a second expression repressor, and a first pharmaceutical composition may comprise the first expression repressor or nucleic acid encoding the same and a second pharmaceutical composition may comprise the second expression repressor or nucleic acid encoding the same.
In some embodiments, pharmaceutical compositions may be specially formulated for administration in solid or liquid form, including those adapted for the following: oral administration, for example, drenches (aqueous or non-aqueous solutions or suspensions), tablets, e.g., those targeted for buccal, sublingual, and systemic absorption, boluses, powders, granules, pastes for application to the tongue; parenteral administration, for example, by subcutaneous, intramuscular, intravenous or epidural injection as, for example, a sterile solution or suspension, or sustained-release formulation; topical application, for example, as a cream, ointment, or a controlled-release patch or spray applied to the skin, lungs, or oral cavity; intravaginally or intrarectally, for example, as a pessary, cream, or foam;
sublingually; ocularly; transdermally; or nasally, pulmonary, and/or to other mucosal surfaces.
As used herein, the term "pharmaceutically acceptable" refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
As used herein, the term "pharmaceutically acceptable carrier" means a pharmaceutically acceptable material, composition, or vehicle, such as a liquid or solid filler, diluent, excipient, or solvent encapsulating material, involved in carrying or transporting the subject compound from one organ, or portion of the body, to another organ, or portion of the body. Each carrier must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not injurious to the patient.
In some embodiments, for example, materials which can serve as pharmaceutically-acceptable carriers include: sugars, such as lactose, glucose and sucrose; starches, such as corn starch and potato starch; cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar;
buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water;
isotonic saline;
Ringer's solution; ethyl alcohol; pH buffered solutions; polyesters, polycarbonates and/or polyanhydrides;
and other non-toxic compatible substances employed in pharmaceutical formulations.
As used herein, the term "pharmaceutically acceptable salt", refers to salts of such compounds that are appropriate for use in pharmaceutical contexts, i.e., salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response, and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge, et al.
describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 66: 1-19 (1977). In some embodiments, pharmaceutically acceptable salts include, but are not limited to, nontoxic acid addition salts, which are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, maleic acid, tartaric acid, citric acid, succinic acid, or malonic acid or by using other methods used in the art such as ion exchange. In some embodiments, pharmaceutically acceptable salts include, but are not limited to, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. In some embodiments, pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl having from 1 to 6 carbon atoms, sulfonate, and aryl sulfonate.
In various embodiments, the present disclosure provides pharmaceutical compositions described herein with a pharmaceutically acceptable excipient. Pharmaceutically acceptable excipient includes an excipient that is useful in preparing a pharmaceutical composition that is generally safe, non-toxic, and desirable, and includes excipients that are acceptable for veterinary use as well as for human pharmaceutical use. Such excipients may be solid, liquid, semisolid, or, in the case of an aerosol composition, gaseous.
Pharmaceutical preparations may be made following conventional techniques of pharmacy involving milling, mixing, granulation, and compressing, when necessary, for tablet forms; or milling, mixing, and filling for hard gelatin capsule forms. When a liquid carrier is used, a preparation can be in the form of a syrup, elixir, emulsion or an aqueous or non-aqueous solution or suspension. Such a liquid formulation may be administered directly per os.
In some embodiments, pharmaceutical compositions may be formulated for delivery to a cell and/or to a subject via any route of administration. Modes of administration to a subject may include injection, infusion, inhalation, intranasal, intraocular, topical delivery, intercannular delivery, or ingestion.
Injection includes, without limitation, intravenous, intramuscular, intra-arterial, intrathecal, intraventricular, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, sub capsular, subarachnoid, intraspinal, intracerebrospinal, and intrasternal injection and infusion. In some embodiments, administration includes aerosol inhalation, e.g., with nebulization. In some embodiments, administration is systemic (e.g., oral, rectal, nasal, sublingual, buccal, or parenteral), enteral (e.g., system-wide effect, but delivered through the gastrointestinal tract), or local (e.g., local application on the skin, intravitreal injection). In some embodiments, one or more compositions is administered systemically. In some embodiments, administration is non-parenteral and a therapeutic is a parenteral therapeutic. In some embodiments, administration may be bronchial (e.g., by bronchial instillation), buccal, dermal (which may be or comprise, for example, one or more of topical to the dermis, intradermal, interdermal, transdermal, etc.), enteral, intra-arterial, intradermal, intragastric, intramedullary, intramuscular, intranasal, intraperitoneal, intrathecal, intravenous, intraventricular, within a specific organ (e. g. intrahepatic), mucosal, nasal, oral, rectal, subcutaneous, sublingual, topical, tracheal (e.g., by intratracheal instillation), vaginal, vitreal, etc. In some embodiments, administration may be a single dose. In some embodiments, administration may involve dosing that is intermittent (e.g., a plurality of doses separated in time) and/or periodic (e.g., individual doses separated by a common period of time) dosing. In some embodiments, administration may involve continuous dosing (e.g., perfusion) for at least a selected period of time.
Pharmaceutical compositions according to the present disclosure may be delivered in a therapeutically effective amount. A precise therapeutically effective amount is an amount of a composition that will yield the most effective results in terms of efficacy of treatment in a given subject. This amount will vary depending upon a variety of factors, including but not limited to characteristics of a therapeutic compound (including activity, pharmacokinetics, pharmacodynamics, and bioavailability), physiological condition of a subject (including age, sex, disease type and stage, general physical condition, responsiveness to a given dosage, and type of medication), nature of a pharmaceutically acceptable carrier or carriers in a formulation, and/or route of administration.
In some aspects, the present disclosure provides methods of delivering a therapeutic comprising administering a composition as described herein to a subject, wherein a genomic complex modulating agent is a therapeutic and/or wherein delivery of a therapeutic causes changes in gene expression relative to gene expression in absence of a therapeutic.
Methods as provided in various embodiments herein may be utilized in any some aspects delineated herein. In some embodiments, one or more compositions is/are targeted to specific cells, or one or more specific tissues.
For example, in some embodiments one or more compositions is/are targeted to epithelial, connective, muscular, and/or nervous tissue or cells. In some embodiments a composition is targeted to a cell or tissue of a particular organ system, e.g., cardiovascular system (heart, vasculature); digestive system (esophagus, stomach, liver, gallbladder, pancreas, intestines, colon, rectum and anus); endocrine system (hypothalamus, pituitary gland, pineal body or pineal gland, thyroid, parathyroids, adrenal glands); excretory system (kidneys, ureters, bladder); lymphatic system (lymph, lymph nodes, lymph vessels, tonsils, adenoids, thymus, spleen); integumentary system (skin, hair, nails); muscular system (e.g., skeletal muscle); nervous system (brain, spinal cord, nerves);
reproductive system (ovaries, uterus, mammary glands, testes, vas deferens, seminal vesicles, prostate); respiratory system (pharynx, larynx, trachea, bronchi, lungs, diaphragm); skeletal system (bone, cartilage); and/or combinations thereof.
In some embodiments, a composition of the present disclosure crosses a blood-brain-barrier, a placental membrane, or a blood-testis barrier.
In some embodiments, a pharmaceutical composition as provided herein is administered systemically.
In some embodiments, administration is non-parenteral and a therapeutic is a parenteral therapeutic.
In some embodiments, a pharmaceutical composition of the present disclosure has improved PK/PD, e.g., increased pharmacokinetics or pharmacodynamics, such as improved targeting, absorption, or transport (e.g., at least 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 75%, 80%, 90% improved or more) as compared to an active agent alone. In some embodiments, a pharmaceutical composition has reduced undesirable effects, such as reduced diffusion to a nontarget location, off-target activity, or toxic metabolism, as compared to a therapeutic alone (e.g., at least 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 75%, 80%, 90% or more reduced, as compared to an active agent alone). In some embodiments, a composition increases efficacy and/or decreases toxicity of a therapeutic (e.g., at least 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 75%, 80%, 90% or more) as compared to an active agent alone.
Pharmaceutical compositions described herein may be formulated for example including a carrier, such as a pharmaceutical carrier and/or a polymeric carrier, e.g., a liposome or vesicle, and delivered by known methods to a subject in need thereof (e.g., a human or non-human agricultural or domestic animal, e.g., cattle, dog, cat, horse, poultry). Such methods include transfection (e.g., lipid-mediated, cationic polymers, calcium phosphate); electroporation or other methods of membrane disruption (e.g., nucleofection) and viral delivery (e.g., lentivirus, retrovirus, adenovirus, AAV). Methods of delivery are also described, e.g., in Gori et al., Delivery and Specificity of CRISPR/Cas9 Genome Editing Technologies for Human Gene Therapy. Human Gene Therapy. July 2015, 26(7): 443-451.
doi:10.1089/hum.2015.074; and Zuris et al. Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol. 2014 Oct 30;33(1):73-80.
Liposomes are spherical vesicle structures composed of a uni- or multilamellar lipid bilayer surrounding internal aqueous compartments and a relatively impermeable outer lipophilic phospholipid bilayer.
Liposomes may be anionic, neutral, or cationic. Liposomes are biocompatible, nontoxic, can deliver both hydrophilic and lipophilic drug molecules, protect their cargo from degradation by plasma enzymes, and transport their load across biological membranes and the blood brain barrier (BBB) (see, e.g., Spuch and Navarro, Journal of Drug Delivery, vol. 2011, Article ID 469679, 12 pages, 2011.
doi:10.1155/2011/469679 for review).
Vesicles can be made from several different types of lipids; however, phospholipids are most commonly used to generate liposomes as drug carriers. Vesicles may comprise without limitation DOTMA, DOTAP, DOTIM, DDAB, alone or together with cholesterol to yield DOTMA
and cholesterol, DOTAP and cholesterol, DOTIM and cholesterol, and DDAB and cholesterol.
Methods for preparation of multilamellar vesicle lipids are known in the art (see for example U.S.
Pat. No. 6,693,086, the teachings of which relating to multilamellar vesicle lipid preparation are incorporated herein by reference). Although vesicle formation can be spontaneous when a lipid film is mixed with an aqueous solution, it can also be expedited by applying force in the form of shaking by using a homogenizer, sonicator, or an extrusion apparatus (see, e.g., Spuch and Navarro, Journal of Drug Delivery, vol. 2011, Article ID 469679, 12 pages, 2011. doi:10.1155/2011/469679 for review).
Extruded lipids can be prepared by extruding through filters of decreasing size, as described in Templeton et al., Nature Biotech, 15:647-652, 1997, the teachings of which relating to extruded lipid preparation are incorporated herein by reference.
Methods and compositions provided herein may comprise a pharmaceutical composition administered by a regimen sufficient to alleviate a symptom of a disease, disorder, and/or condition. In some aspects, the present disclosure provides methods of delivering a therapeutic by administering compositions as described herein.
Uses The present disclosure is further directed to uses of the expression repression systems disclosed herein. Among other things, in some embodiments such provided technologies may be used to achieve modulation, e.g., repression, of target gene expression and, for example, enable control of target gene activity, delivery, and penetrance, e.g., in a cell. In some embodiments, a cell is a mammalian, e.g., human, cell. In some embodiments, a cell is a somatic cell. In some embodiments, a cell is a primary cell.
For example, in some embodiments, a cell is a mammalian somatic cell. In some embodiments, a mammalian somatic cell is a primary cell. In some embodiments, a mammalian somatic cell is a non-embryonic cell.
Dosage The dosage of the administered agent or composition can vary based on, e.g., the condition being treated, the severity of the disease, the subject's individual parameters, including age, physiological condition, size and weight, duration of treatment, the type of treatment to be performed (if any), the particular route of administration and similar factors. Thus, the dose administered of the agents described herein can depend on such various parameters. The dosage of an administered composition may also vary depending upon other factors as the subject's sex, general medical condition, and severity of the disorder to be treated. It may be desirable to provide the subject with a dosage of an expression repressor or an expression repression system or combination of expression repressors disclosed herein as circumstances dictate. In some embodiments, the subject is provided with a dosage of an expression repressor, or an expression repression system or combination of expression repressors disclosed herein as circumstances dictate.
Pharmaceutical compositions according to the present disclosure may be delivered in a therapeutically effective amount. A precise therapeutically effective amount is an amount of a composition that will yield the most effective results in terms of efficacy of treatment in a given subject. This amount will vary depending upon a variety of factors, including but not limited to characteristics of a therapeutic compound (including activity, pharmacolcinetics, pharmacodynamics, and bioavailability), physiological condition of a subject (including age, sex, disease type and stage, general physical condition, responsiveness to a given dosage, and type of medication), nature of a pharmaceutically acceptable carrier or carriers in a formulation, and/or route of administration.
Lipid Nanoparticles Expression repressors or expression repression systems as described herein can be delivered using any biological delivery system/formulation including a particle, for example, a nanoparticle delivery system.
Nanoparticles include particles with a dimension (e.g. diameter) between about 1 and about 1000 nanometers, between about 1 and about 500 nanometers in size, between about 1 and about 100 nm, between about 30 nm and about 200 nm, between about 50 nm and about 300 nm, between about 75 nm and about 200 nm, between about 100 nm and about 200 nm, and any range therebetween. A nanoparticle has a composite structure of nanoscale dimensions. In some embodiments, nanoparticles are typically spherical although different morphologies are possible depending on the nanoparticle composition. The portion of the nanoparticle contacting an environment external to the nanoparticle is generally identified as the surface of the nanoparticle. In some embodiments, nanoparticles have a greatest dimension ranging between 25 nm and 200 nm. Nanoparticles as described herein comprise delivery systems that may be provided in any form, including but not limited to solid, semi-solid, emulsion, or colloidal nanoparticles.
A nanoparticle delivery system may include but not limited to lipid-based systems, liposomes, micelles, micro-vesicles, exosomes, or gene gun. In one embodiment, the nanoparticle is a lipid nanoparticle (LNP). In some embodiments, the LNP is a particle that comprises a plurality of lipid molecules physically associated with each other by intermolecular forces.
In some embodiments, an LNP may comprise multiple components, e.g., 3-4 components. In one embodiment, the expression repressor or a pharmaceutical composition comprising said expression repressor (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repressor nucleic acid) is encapsulated in an LNP. In one embodiment, the expression repression system or a pharmaceutical composition comprising said expression repression system (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repression system nucleic acid) is encapsulated in an LNP. In some embodiments, the nucleic acid encoding the first expression repressor and the nucleic acid encoding the second expression repressor are present in same LNP. In some embodiments, the nucleic acid encoding the first expression repressor and the nucleic acid encoding the second expression repressor are present in different LNPs. Preparation of LNPs and the modulating agent encapsulation may be used/and or adapted from Rosin et al, Molecular Therapy, vol. 19, no. 12, pages 1286-2200, December 2011). In some embodiments, lipid nanoparticle compositions disclosed herein are useful for expression of protein encoded by mRNA. In some embodiments, nucleic acids, when present in the lipid nanoparticles, are resistant in aqueous solution to degradation with a nuclease.
In some embodiments, the LNP formulations may include a CCD lipid, a neutral lipid, and/or a helper lipid. In some embodiments, the LNP formulation comprises an ionizable lipid. In some embodiments, an ionizable lipid may be a cationic lipid, an ionizable cationic lipid, or an amine-containing lipid that can be readily protonated. In some embodiments, the lipid is a cationic lipid that can exist in a positively charged or neutral form depending on pH. In some embodiments, the cationic lipid is a lipid capable of being positively charged, e.g., under physiological conditions. In some embodiments, the lipid particle comprises a cationic lipid in formulation with one or more of neutral lipids, ionizable amine-containing lipids, biodegradable alkyn lipids, steroids, phospholipids including polyunsaturated lipids, structural lipids (e.g., sterols), PEG, cholesterol, and polymer conjugated lipids.
In some embodiments, LNP formulation (e.g., MC3 and/or SSOP) includes cholesterol, PEG, and/or a helper lipid. The LNPs may be, e.g., microspheres (including unilamellar and multilamellar vesicles, lamellar phase lipid bilayers that, in some embodiments, are substantially spherical.
In some embodiments, the LNP can comprise an aqueous core, e.g., comprising a nucleic acid encoding an expression repressor or a system as disclosed herein. In some embodiments of the present disclosure, the cargo for the LNP formulation includes at least one guide RNA. In some embodiments, the cargo, e.g., a nucleic acid encoding an expression repressor, or a system as disclosed herein, may be adsorbed to the surface of an LNP, e.g., an LNP comprising a cationic lipid. In some embodiments, the cargo, e.g., a nucleic acid encoding an expression repressor, or a system as disclosed herein may be associated with the LNP. In some embodiments, the cargo, e.g., a nucleic acid encoding an expression repressor, or a system as disclosed herein, may be encapsulated, e.g., fully encapsulated and/or partially encapsulated in an LNP.
In some embodiments, an LNP comprising a cargo may be administered for systemic delivery, e.g., delivery of a therapeutically effective dose of cargo that can result in a broad exposure of an active agent within an organism. Systemic delivery of lipid nanoparticles can be by any means known in the art including, for example, intravenous, intraarterial, subcutaneous, and intraperitoneal delivery. In some embodiments, systemic delivery of lipid nanoparticles is by intravenous delivery. In some embodiments, an LNP comprising a cargo may be administered for local delivery, e.g., delivery of an active agent directly to a target site within an organism.
The LNPs may be formulated as a dispersed phase in an emulsion, micelles, or an internal phase in a suspension. In some embodiments, the LNPs are biodegradable. In some embodiments, the LNPs do not accumulate to cytotoxic levels or cause toxicity in vivo at a therapeutically effective dose. In some embodiments, the LNPs do not accumulate to cytotoxic levels or cause toxicity in vivo after repeat administrations at a therapeutically effective dose. In some embodiments, the LNPs do not cause an innate immune response that leads to a substantially adverse effect at a therapeutically effective dose.
In some embodiments, the LNP used, comprises the formula (6Z,9Z,28Z,31Z)-heptatriacont-6,9,28,31-tetraene-19-y1 4-(dimethylamino) butanoate or ssPalm0-phenyl-P4C2 (ssPalmO-Phe, SS-OP). In some embodiments, the LNP formulation comprises the formula, (6Z,9Z,28Z,31Z)-heptatriacont-6,9,28,31-tetraene-19-y1 4-(dimethylamino)butanoate (MC3), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), Cholesterol, 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (PEG2k-DMG), e.g., MC3 LNP or ssPalm0-phenyl-P4C2 (ssPalmO-Phe, SS-OP), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), Cholesterol, 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (PEG2k-DMG), e.g., SSOP-LNP.
Liposomes are spherical vesicle structures composed of a uni- or multilamellar lipid bilayer surrounding internal aqueous compartments and a relatively impermeable outer lipophilic phospholipid bilayer.
Liposomes may be anionic, neutral, or cationic. Liposomes are biocompatible, nontoxic, can deliver both hydrophilic and lipophilic drug molecules, protect their cargo from degradation by plasma enzymes, and transport their load across biological membranes and the blood brain barrier (BBB) (see, e.g., Spuch and Navarro, Journal of Drug Delivery, vol. 2011, Article ID 469679, 12 pages, 2011.
doi:10.1155/2011/469679 for review).
Vesicles can be made from several different types of lipids; however, phospholipids are most commonly used to generate liposomes as drug carriers. Vesicles may comprise without limitation DOTMA, DOTAP, DOTIM, DDAB, alone or together with cholesterol to yield DOTMA
and cholesterol, DOTAP and cholesterol, DOTIM and cholesterol, and DDAB and cholesterol.
Methods for preparation of multilamellar vesicle lipids are known in the art (see for example U.S.
Pat. No. 6,693,086, the teachings of which relating to multilamellar vesicle lipid preparation are incorporated herein by reference). Although vesicle formation can be spontaneous when a lipid film is mixed with an aqueous solution, it can also be expedited by applying force in the form of shaking by using a homogenizer, sonicator, or an extrusion apparatus (see, e.g., Spuch and Navarro, Journal of Drug Delivery, vol. 2011, Article ID 469679, 12 pages, 2011. doi:10.1155/2011/469679 for review).
Extruded lipids can be prepared by extruding through filters of decreasing size, as described in Templeton et al., Nature Biotech, 15:647-652, 1997, the teachings of which relating to extruded lipid preparation are incorporated herein by reference.
Methods and compositions provided herein may comprise a pharmaceutical composition administered by a regimen sufficient to alleviate a symptom of a disease, disorder, and/or condition. In some aspects, the present disclosure provides methods of delivering a therapeutic by administering compositions as described herein.
Modulating Gene Expression The present disclosure is further directed, in part, to a method of modulating, e.g., decreasing, expression of a target gene, comprising providing an expression repressor (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repressor nucleic acid) or an expression repression system described herein (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repression system or nucleic acid), and contacting the target gene, and/or operably linked transcription control element(s) with the expression repressor or the expression repression system. In some embodiments, modulating, e.g., decreasing expression of a target gene, comprises modulation of transcription of a target gene, as compared with a reference value, e.g., transcription of a target gene, in absence of the expression repressor or the expression repression system. In some embodiments, the method of modulating, e.g., decreasing, expression of a target gene, are used ex vivo, e.g., on a cell from a subject, e.g., a mammalian subject, e.g., a human subject. In some embodiments, the method of modulating, e.g., decreasing, expression of a target gene, are used in vivo, e.g., on a mammalian subject, e.g., a human subject. In some embodiments, the method of modulating, e.g., decreasing, expression of a target gene, are used in vitro, e.g., on a cell or cell line described herein.
The present disclosure is further directed, in part to a method of treating a condition associated with over-expression of a target gene in a subject, comprising administering to the subject an expression repression system described herein (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repression system or nucleic acid). Conditions associated with over-expression of particular genes are known to those of skill in the art. Such conditions include, but are not limited to, metabolic disorders, neuromuscular disorders, cancer (e.g., solid tumors), fibrosis, diabetes, urea disorders, immune disorders, inflammation, and arthritis.
The present disclosure is further directed, in part to a method of treating a condition associated with mis-regulation of the expression of a target gene in a subject, comprising administering to the subject an expression repression system described herein (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repression system or nucleic acid).
Without wishing to be bound by theory, it is thought that an expression repression system may be used to target (e.g., decrease expression of) a gene which modulates the expression of a target gene, thus altering expression of the target gene by altering expression of the modulating gene. Conditions associated with mis-regulation of the expression of particular genes are known to those of skill in the art.
Such conditions include, but are not limited to metabolic disorders, neuromuscular disorders, cancer (e.g., solid tumors), fibrosis, diabetes, urea disorders, immune disorders, inflammation, and arthritis.
Methods and compositions as provided herein may treat a condition associated with over-expression or mis-regulation of a target gene by stably or transiently altering (e.g., decreasing) transcription of a target gene. In some embodiments, such a modulation persists for at least about 1 hr to about 30 days, or at least about 2 hrs, 6 hrs, 12 hrs, 18 hrs, 24 hrs, 2 days, 3, days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 21 days, 22 days, 23 days, 24 days, 25 days, 26 days, 27 days, 28 days, 29 days, 30 days, or longer or any time therebetween. In some embodiments, such a modulation persists for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6, or 7 days, or at least 1, 2, 3, 4, or 5 weeks, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely). Optionally, such a modulation persists for no more than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 years.
In some embodiments, a method or composition provided herein may decrease expression of a target gene in a cell by at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100%
(and optionally up to 100%) relative to expression of the target gene in a cell not contacted by the composition or treated with the method.
In some embodiments, a method provided herein may modulate, e.g., decrease, expression of a target gene by disrupting a genomic complex, e.g., an anchor sequence-mediated conjunction, associated with said target gene. A gene that is associated with an anchor sequence-mediated conjunction may be at least partially within a conjunction (that is, situated sequence-wise between a first and second anchor sequences), or it may be external to a conjunction in that it is not situated sequence-wise between a first and second anchor sequences, but is located on the same chromosome and in sufficient proximity to at least a first or a second anchor sequence such that its expression can be modulated by controlling the topology of the anchor sequence-mediated conjunction. Those of ordinary skill in the art will understand that distance in three-dimensional space between two elements (e.g., between the gene and the anchor sequence-mediated conjunction) may, in some embodiments, be more relevant than distance in terms of base pairs. In some embodiments, an external but associated gene is located within 2 Mb, within 1.9 Mb, within 1.8 Mb, within 1.7 Mb, within 1.6 Mb, within 1.5 Mb, within 1.4 Mb, with 1.3 Mb, within 1.3 Mb, within 1.2 Mb, within 1.1 Mb, within 1 Mb, within 900 kb, within 800 kb, within 700 kb, within 500 kb, within 400 kb, within 300 kb, within 200 kb, within 100 kb, within 50 kb, within 20 kb, within 10 kb, or within 5 kb of the first or second anchor sequence.
In some embodiments, modulating expression of a gene comprises altering accessibility of a transcriptional control sequence to a gene. A transcriptional control sequence, whether internal or external to an anchor sequence-mediated conjunction, can be an enhancing sequence or a silencing (or repressive) sequence.
Epigenetic Modification The present disclosure is further directed, in part, to a method of epigenetically modifying a target gene, a transcription control element operably linked to a target gene, or an anchor sequence (e.g., an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene), the method comprising providing an expression repression system (e.g., expression repressor(s)), or nucleic acid encoding the same or pharmaceutical composition comprising said expression repression system or nucleic acid; and contacting the target gene or a transcription control element operably linked to the target gene with the expression repression system, thereby epigenetically modifying the target gene or a transcription control element operably linked to the target gene.
In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises increasing or decreasing DNA methylation of the target gene or a transcription control element operably linked to a target gene. In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises increasing or decreasing histone methylation of a histone associated with the target gene or a transcription control element operably linked to a target gene. In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises decreasing histone acetylation of a histone associated with the target gene, or a transcription control element operably linked to a target gene. In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene .. comprises increasing or decreasing histone sumoylation of a histone associated with the target gene or a transcription control element operably linked to a target gene. In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises increasing or decreasing histone phosphorylation of a histone associated with the target gene or a transcription control element operably linked to a target gene.
In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene may decrease the level of the epigenetic modification by at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% (and optionally up to 100%) relative to the level of the epigenetic modification at that site in a cell not contacted by the composition or treated with the method.
In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene may increase the level of the epigenetic modification by at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 120, 140, 160, 180, 200, 300, 400, 500, 600, 700, 800, 900, or 1000% (and optionally up to 200, 300, 400, 500, 600, 700, 800, 900, 1000, or 2000%) relative to the level of the epigenetic modification at that site in a cell not contacted by the composition or treated with the method. In some embodiments epigenetic modification of a target gene or a transcription control element operably linked to a target gene may modify the level of expression of the target gene, e.g., as described herein.
In some embodiments, an epigenetic modification produced by a method described herein persists for at least about 1 hr to about 30 days, or at least about 2 hrs, 6 hrs, 12 hrs, 18 hrs, 24 hrs, 2 days, 3, days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 21 days, 22 days, 23 days, 24 days, 25 days, 26 days, 27 days, 28 days, 29 days, 30 days, or longer or any time therebetween. In some embodiments, such a modulation persists for at least 1, 2, 3,4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6, or 7 days, or at least 1,2, 3, 4, or 5 weeks, or at least 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely).
Optionally, such a modulation persists for no more than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 years.
In some embodiments, an expression repression system for use in a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises an expression repressor comprising a repressor domain that is or comprises an epigenetic modifying moiety.
For example, a repressor domain may be or comprise an epigenetic modifying moiety with DNA
methyltransferase activity, and an endogenous or naturally occurring target sequence (e.g. a target gene or transcription control element) may be altered to increase its methylation (e.g., decreasing interaction of a transcription factor with a portion of target gene or transcription control element, decreasing binding of a nucleating protein to an anchor sequence, and/or disrupting or preventing an anchor sequence-mediated conjunction), or may be altered to decrease its methylation (e.g., increasing interaction of a transcription factor with a portion of a target gene or transcription control element, increasing binding of a nucleating protein to an anchor sequence, and/or promoting or increasing strength of an anchor sequence-mediated conjunction).
Kits The present disclosure further directed, in part, to a kit comprising an expression repressor or an expression repression system, e.g., expression repressor(s), described herein.
In some embodiments, a kit comprises an expression repressor or an expression repression system (e.g., the expression repressor(s) of the expression repression system) and instructions for the use of said expression repressor or expression repression system. In some embodiments, a kit comprises a nucleic acid encoding the expression repressor or the expression repression system or a component thereof (e.g., the expression repressor(s) of the expression repression system) and instructions for the use of said nucleic acid and/or said expression repressor or the expression repression system. In some embodiments, a kit comprises a cell comprising a nucleic acid encoding the expression repressor or the expression repression system or a component thereof (e.g., the expression repressor(s) of the expression repression system) and instructions for the use of said cell, nucleic acid, and/or said expression repression system.
In some embodiments, a kit comprises a unit dosage of an expression repressor or an expression repression system, e.g., expression repressor(s), described herein, or a unit dosage of a nucleic acid, e.g., a vector, encoding an expression repressor or an expression repression system, e.g., expression repressor(s), described herein.
In some embodiments the kit further comprises b) a set of instructions comprising at least one method for treating a disease or modulating, e.g., decreasing the expression of target gene within a cell with said composition. In some embodiments, the kits can optionally include a delivery vehicle for said composition (e.g., a lipid nanoparticle). The reagents may be provided suspended in the excipient and/or delivery vehicle or may be provided as a separate component which can be later combined with the excipient and/or delivery vehicle. In some embodiments, the kits may optionally contain additional therapeutics to be co-administered with the compositions to affect the desired target gene expression.
While the instructional materials typically comprise written or printed materials, they are not limited to such. Any medium capable of storing such instructions and communicating them to an end user is contemplated. Such media include but are not limited to electronic storage media (e.g., magnetic discs, tapes, cartridges, chips), optical media (e.g., CD ROM), and the like. Such media may include addresses to internet sites that provide such instructional materials.
In some embodiments, a kit comprises a unit dosage of an expression repressor an expression repression system, e.g., expression repressor(s), described herein, or a unit dosage of a nucleic acid, e.g., a vector, encoding an expression repression system, e.g., expression repressor(s), described herein.
The following examples are provided to further illustrate some embodiments of the present disclosure but are not intended to limit the scope of the disclosure; it will be understood by their exemplary nature that other procedures, methodologies, or techniques known to those skilled in the art may alternatively be used.
EXAMPLES
Example 1: Target Selection and Guide Design The I3-2-microglobulin (I32M) gene was targeted for down-regulation using an expression repression system of the disclosure. A first expression repressor comprising an S. pyo genes dCas9 and a KRAB domain (Sp-dCas9-KRAB, corresponding to SEQ ID NOs: 91-93) and a second expression repressor comprising S. aureus dCas9 and MQ1 (Sa-dCas9-MQ1, corresponding to SEQ ID Nos: 94-96) were used. Guide RNAs (comprising target-binding sequences corresponding to SEQ ID NOs: 1-5) targeted each expression repressor to an area of CpG hypomethylation in the promoter region of the I32M
gene (Figure 1), where methylation runs from 0 to 1 (0 to 100%) and where higher methylation is depicted by red bars. Each bar represents a CpG site. Blue guides (GD-28228 and GD-28229) were used to target Sp-dCas9-KRAB and orange guides (GD-28171, GD-28172 and GD-28173) were used to target Sa-dCas9-MQ1 to the CpG Island in the I32M promoter region.
Example 2: I32M Expression Repression Using One or Two Expression Repressors The guide RNAs of Example 1 were used to target the Sp-dCas9-KRAB expression repressor, the Sa-dCas9-MQ1 expression repressor, or both to the promoter region of I32M and the effects on I32M
expression monitored by qPCR. mRNA encoding the expression repressor(s) and guide RNA(s) were delivered to cells in lipid nanoparticles (LNPs).
HepG2 cells were seeded in a 96 well plate at a density of 30,000 cells/well in 100 tit RPMI (+10% FBS, +Penn/Strep). Cells were allowed to attach for 24 hours prior to treatment with 0.125 ug RNA (1:1 mRNA: sgRNA by weight) per guide. RNA was delivered by LNP formulated with COATSOME SS-OP
ionizable lipid (NOF America). LNPs were formulated with one mRNA and one guide, with the exception of the samples noted as "co-formulated" which were prepared with two guides, maintaining the same mRNA: guide weight ratio. Cells were incubated with the LNPs for 72 hours, following which the cells were harvested for I32M mRNA analysis by qPCR (Figure 2), with I32M mRNA
levels given relative to untreated control cells.
dCas9 alone targeted to the I32M promoter did not decrease I32M mRNA levels.
The data shows that Sp-dCas9-KRAB or Sa-dCas9-MQ1 alone decreased I32M mRNA levels, with some variation in the decrease depending on the guide RNA(s) used. Sp-dCas9-KRAB and Sa-dCas9-MQ1 together decreased I32M
mRNA levels on average more than either expression repressor alone, again with some variation in the decrease depending on the guide RNA(s) used. Use of an expression repression system comprising one or two expression repressors decreased expression of a target gene The expression repression system comprising two expression repressors appeared to decrease expression of the target gene more than a single expression repressor.
Example 3: I32M Expression Repression Across Doses and Using Flow Cytometry Guide RNA of Example 1 (GD-28228 for Sp-dCas9 and Sp-dCas9-KRAB; GD-28172 for Sa-dCas9-MQ1; or GD-28228 and GD-28172 for Sp-dCas9-KRAB and Sa-dCas9-MQ1) was used to target the Sp-dCas9-KRAB expression repressor, the Sa-dCas9-MQ1 expression repressor, or both to the promoter region of I32M and the effects on I32M expression monitored by qPCR and also by flow cytometry (using a fluorescent I32M to monitor I32M protein levels). mRNA encoding the expression repressor(s) and guide RNA(s) were delivered to cells in LNPs at different dosages and formulations.
HepG2 cells were seeded in a 12-well plate at a density of 330,000 cells/well 1.5 mL RPMI (+10% FBS, +Penn/Strep). Cells were allowed to attach for 24 hours prior to treatment with 1.25 ug or 2.5 ug RNA
(1:1 mRNA: sgRNA by weight) per guide in 1 mL media. RNA was delivered by LNP
formulated with COATSOME SS-OP ionizable lipid (NOF America). LNPs were formulated with one mRNA and one guide, with the exception of the samples noted as "co-formulated" which were prepared with two guides, maintaining the same mRNA: guide weight ratio. Cells were incubated with the LNPs for 72 hours, following which the cells were harvested for FACS and mRNA analysis by qPCR.
As seen in Example 2, dCas9 alone targeted to the I32M promoter did not decrease I32M mRNA levels.
Sp-dCas9-KRAB or Sa-dCas9-MQ1 alone, and Sp-dCas9-KRAB and Sa-dCas9-MQ1 together decreased I32M mRNA levels (Figure 3). Both 1.25 ug/ml and 2.5 ug/ml doses of the expression repressors (alone or together) decreased I32M mRNA levels, with the higher dose of Sp-dCas9-KRAB
appearing to give a greater decrease than the lower dose. FACS monitoring of I32M protein levels showed that Sp-dCas9-KRAB or Sa-dCas9-MQ1 treated cells showed a decrease in the population of cells having the untreated level of I32M protein and the appearance of a new population of cells with a decreased level of I32M
protein. These data are consistent with those of Example 2, showing that use of an expression repression system comprising one or two expression repressors decreases expression of a target gene, and that individual expression repressors decrease the levels of a protein encoded by the target gene too.
Example 4: I32M Expression Repression Over Time Guide RNA (GD-28228 for Sp-dCas9 and Sp-dCas9-KRAB; GD-28172 for Sa-dCas9-MQ1;
or GD-28228 and GD-28172 for Sp-dCas9-KRAB and Sa-dCas9-MQ1) of Example 1 was used to target the Sp-dCas9-KRAB expression repressor, the Sa-dCas9-MQ1 expression repressor, or both to the promoter region of I32M and the effects on I32M expression monitored by qPCR. Cells were collected at different days after transfection to extract total RNA and probe for expression changes.
K562 cells were plated in a 24-well plate and transfected with MC3-based LNP
formulation enclosing:
dCas9 mRNA with I32M targeting guide RNA, Sp-dCas9-KRAB mRNA with I32M
targeting guide RNA, Sa-dCas9-MQ1 mRNA with I32M targeting guide RNA, or a mixture of Sp-dCas9-KRAB
and Sa-dCas9-MQ1 mRNAs with I32M targeting guide RNAs. Cells were collected at different days after transfection to extract total RNA and probe for expression changes in I32M mRNA (Figure 4).
Dotted lines indicate average untreated I32M mRNA levels over time (top) and average knockdown levels of I32M mRNA 24 hours post transfection (bottom).
Over a 22-day period, non-transfected (blue line) and dCas9 transfected (red line) cells don't change I32M
expression, whereas Sp-dCas9-KRAB (green line) transfected cells have lower I32M mRNA levels in the initial days post-transfection, with maximum effect on day 2, but the repression is lost after day 5. Sa-dCas9-MQ1 (purple line) transfected cells show significant repression (up to 85% by day 4) of I32M
mRNA levels and the repression is retained and then slowly the repression decreases somewhat as seen by the line remaining just under the dotted line in the figure at the end of the test period. Sp-dCas9-KRAB
and Sa-dCas9-MQ1 combined transfection (orange line) did not give any added repression than what is already observed for Sa-dCas9-MQ1 alone (purple line).
These data show that use of an expression repression system comprising one or two expression repressors decreases expression of a target gene. Individual expression repressors such as those comprising MQ1 can repress expression of a target gene for an extended duration, and expression repression systems comprising more than one expression repressor, e.g., one of which is an expression repressor comprising MQ1, can repress expression of a target gene for an extended duration as well.
Example 5: Durable repression of B2M expression after promoter methylation by DNA
methyltransferases This example demonstrates methylation of B2M promoter region by treatment with LNPs containing various DNMTs and LNPs containing a guide targeting the dCas9 fusions to the B2M
promoter region cause durable repression of B2M expression.
K-562 cells were seeded in 24 well plates at a density of 200,000 cells per well in 750 tit of growth medium (RPMI + 10% FBS + pen/strep). mRNAs encoding effectors, dCas9-MQ1(SEQ ID NO:
33), dCas9-DNMT3a/3L (m) ("m" denoting that the 3a region has the human sequence but the 3L region has the mouse sequence) (SEQ ID NO:36), dCas9-DNMT3a/3L (h ("h" denoting that both the 3a region and the 3L region have the human sequences) (SEQ ID NO:35), dCas9-DNMT1(SEQ ID
NO: 35), and dCas9-DNMT3B were formulated in MC3 lipids using a Precision NanoSystems NanoAssemblr Spark instrument. Single guide RNAs (sgRNAs) of SEQ ID NO: 21 were formulated in the same lipids in the same instrument in separate formulations. Stock LNP concentrations were 100 tig/mL. Cells were treated with LNPs encapsulating mRNAs and LNPs encapsulating sgRNAs in a 1:1 ratio at a final lipid concentration of 1.25 tig/mL and incubated thereafter. Each effector and guide combination were transfected in quadruplicate. At different timepoints after transfection, 200 tiL of cells were removed and processed to extract total RNA and probe for expression changes and 200 tit of cells were moved into 550 tit of fresh media. This procedure continued until 18 days after the initial transfection. RNA was extracted using the RNeasy 96 Plus kits (Qiagen), converted to cDNA using LunaScript RT SuperMix (New England Biolabs) and used for RT-qPCR using a B2M specific TaqMan primer/probe set Assay with and the TaqMan' Fast Advanced Master Mix (Thermo Fisher Scientific). Data is presented as fold change compared to un-transfected control using the ddCT method.
Results show, all the constructs described in this Example were able to repress B2M expression ranging from about 35% to 60%, 4 days after treatment relative to an untreated sample (Figure 5). All the constructs were able to repress B2M expression ranging from about 15% to 30%
at the end of 8 days relative to an untreated sample. At the end of 13 days, all effectors were able to repress B2M expression by about 45-55%, and at the end of 18 days, all effectors were able to repress B2M expression by about 30-40% relative to an untreated sample (Figure 5).
Example 6: Identification of durable repressors or repressor systems that downregulate MYC
expression by targeting a CTCF site located upstream of MYC gene in K-562 cells This example demonstrates identification of individual expression repressors or expression repression systems that downregulate MYC expression by targeting the CTCF site located upstream of MYC gene.
K-562 cells were grown in 96 well plates at a density of 1 x106 cell/mL in 100 tiL of growth medium (RPMI + 10% FBS + pen/strep). A sgRNA pool was prepared by mixing GD-29639 (SEQ ID
NO: 11) and GD-29640 (SEQ ID NO: 12) in 1:1 ratio. A repression master plate was prepared by adding 5 iu.1 of mRNAs encoding effectors or effector combinations from Table 4 in each well. After addition, 17.5 ng of each mRNA or combination of mRNAs were present in each well. After plating the master plates were stored frozen until the time of transfection.
Table 4: List of Expression repressors or expression repression systems tested Expression repressors or expression repression systems 1 No effector (Untreated) 2 dCas9 3 dCas9-DNMT1 4 dCas9-DNMT3B
5 G9A-dCas9 6 dCas9-MQ1 7 dCas9-HDAC8 8 dCas9-DNMT3a/3L (h) 9 dCas9-DNMT3a/3L (m) EZH2-dCas9 11 dCas9-LSD1 12 dCas9-KRAB
13 EZH2-dCas9-KRAB
14 G9A-dCas9-KRAB
FOG1-dCas9- FOG1 16 dCas9-KRAB + FOG1-dCas9- FOG1 17 EZH2-dCas9-KRAB + dCas9-HDAC8 18 dCas9-LSD1 + dCas9-HDAC8 19 G9A-dCas9-KRAB + EZH2-dCas9-KRAB
G9A-dCas9 + dCas9-HDAC8 21 EZH2-dCas9 + dCas9-HDAC8 22 dCas9-LSD1 + G9A-dCas9-KRAB
23 dCas9-LSD1 + EZH2-dCas9-KRAB
24 dCas9-LSD1 + EZH2-dCas9 G9A-dCas9-KRAB + dCas9-HDAC8
234. The cell of embodiment 233, wherein the cell does not comprise the expression repression system, e.g., at least one component (e.g., all components) of the expression repression system.
30 235. The cell of either of embodiments 233 or 234, wherein expression of the target gene is decreased relative to a cell not contacted, treated, or produced by the method of any of embodiments 209-228 or 230.
236. A kit comprising a container comprising a composition comprising an expression repressor, an expression repression system, one or more nucleic acids encoding said expression repressor or an expression repression system, a vector, a lipid nanoparticle, or a pharmaceutical composition of any of embodiments 1-208 and a set of instructions comprising at least one method for modulating, e.g., .. decreasing the expression of a gene within a cell with said composition.
DEFINITIONS
Anchor Sequence: The term "anchor sequence" as used herein, refers to a nucleic acid sequence recognized by a nucleating agent that binds sufficiently to form an anchor sequence-mediated conjunction, e.g., a complex. In some embodiments, an anchor sequence comprises one or more CTCF
binding motifs. In some embodiments, an anchor sequence is not located within a gene coding region. In some embodiments, an anchor sequence is located within an intergenic region.
In some embodiments, an anchor sequence is not located within either of an enhancer or a promoter. In some embodiments, an anchor sequence is located at least 400 bp, at least 450 bp, at least 500 bp, at least 550 bp, at least 600 bp, at least 650 bp, at least 700 bp, at least 750 bp, at least 800 bp, at least 850 bp, at least 900 bp, at least 950 bp, or at least lkb away from any transcription start site. In some embodiments, an anchor sequence is located within a region that is not associated with genomic imprinting, monoallelic expression, and/or monoallelic epigenetic marks. In some embodiments, the anchor sequence has one or more functions selected from binding an endogenous nucleating polypeptide (e.g., CTCF), interacting with a second anchor sequence to form an anchor sequence mediated conjunction, or insulating against an enhancer that is outside the anchor sequence mediated conjunction. In some embodiments of the present disclosure, technologies are provided that may specifically target a particular anchor sequence or anchor sequences, without targeting other anchor sequences (e.g., sequences that may contain a nucleating agent (e.g., CTCF) binding motif in a different context); such targeted anchor sequences may be referred to as the "target anchor sequence". In some embodiments, sequence and/or activity of a target anchor sequence is modulated while sequence and/or activity of one or more other anchor sequences that may be present in the same system (e.g., in the same cell and/or in some embodiments on the same nucleic acid molecule ¨
e.g., the same chromosome) as the targeted anchor sequence is not modulated.
In some embodiments, the anchor sequence comprises or is a nucleating polypeptide binding motif. In some embodiments, the anchor sequence is adjacent to a nucleating polypeptide binding motif.
Anchor Sequence-Mediated Conjunction: The term "anchor sequence-mediated conjunction" as used herein, refers to a DNA structure, in some cases, a complex, that occurs and/or is maintained via physical interaction or binding of at least two anchor sequences in the DNA by one or more polypeptides, such as nucleating polypeptides, or one or more proteins and/or a nucleic acid entity (such as RNA or DNA), that bind the anchor sequences to enable spatial proximity and functional linkage between the anchor sequences (see, e.g. Figure 1).
Associated with: Two events or entities are "associated" with one another, as that term is used herein, if presence, level, form and/or function of one is correlated with that of the other. For example, in some embodiments, a particular entity (e.g., polypeptide, genetic signature, metabolite, microbe, etc.) is considered to be associated with a particular disease, disorder, or condition, if its presence, level, form and/or function correlates with incidence of and/or susceptibility to the disease, disorder, or condition (e.g., across a relevant population). In some embodiments, two or more entities are physically "associated" with one another if they interact, directly or indirectly, so that they are and/or remain in physical proximity with one another. In some embodiments, two or more entities that are physically associated with one another are covalently linked to one another; in some embodiments, two or more entities that are physically associated with one another are not covalently linked to one another but are .. non-covalently associated, for example by means of hydrogen bonds, van der Waals interaction, hydrophobic interactions, magnetism, and combinations thereof. In some embodiments, a DNA sequence is "associated with" a target genomic or transcription complex when the nucleic acid is at least partially within the target genomic or transcription complex, and expression of a gene in the DNA sequence is affected by formation or disruption of the target genomic or transcription complex.
Domain: As used herein, the term "domain" refers to a section or portion of an entity. In some embodiments, a "domain" is associated with a particular structural and/or functional feature of the entity so that, when the domain is physically separated from the rest of its parent entity, it substantially or entirely retains the particular structural and/or functional feature.
Alternatively, or additionally, in some embodiments, a domain may be or include a portion of an entity that, when separated from that (parent) entity and linked with a different (recipient) entity, substantially retains and/or imparts on the recipient entity one or more structural and/or functional features that characterized it in the parent entity. In some embodiments, a domain is or comprises a section or portion of a molecule (e.g., a small molecule, carbohydrate, lipid, nucleic acid, polypeptide, etc.). In some embodiments, a domain is or comprises a section of a polypeptide. In some such embodiments, a domain is characterized by a particular structural element (e.g., a particular amino acid sequence or sequence motif, alpha-helix character, beta-sheet character, coiled-coil character, random coil character, etc.), and/or by a particular functional feature (e.g., binding activity, enzymatic activity, folding activity, signaling activity, etc.).
Expression repressor: As used herein, the term "expression repressor" refers to an agent or entity with one or more functionalities that decrease expression of a target gene in a cell and that specifically binds to a DNA sequence (e.g., a DNA sequence associated with a target gene, or a transcription control element operably linked to a target gene). An expression repressor comprises at least one DNA-targeting moiety and at least one repressor domain.
Expression repression system: As used herein, the term "expression repression system" refers to a plurality of expression repressors which decrease expression of a target gene in a cell. In some embodiments, an expression repression system comprises a first expression repressor and a second expression repressor, wherein the first expression repressor and second expression repressor (or nucleic acids encoding the first expression repressor and second expression repressor) are present together in a single composition, mixture, or pharmaceutical composition. In some embodiments, an expression repression system comprises a first expression repressor and a second expression repressor, wherein the first expression repressor and second expression repressor (or nucleic acids encoding the first expression repressor and second expression repressor) are present in separate compositions or pharmaceutical compositions. In some embodiments, the first expression repressor and the second expression repressor are present in the same cell at the same time. In some embodiments, the first expression repressor and the second expression repressor are not present in the same cell at the same time, e.g., they are present sequentially. For example, the first expression repressor may be present in a cell for a first time period, and then the second expression repressor may be present in the cell for a second time period, wherein the first and second time periods may be overlapping or non-overlapping.
Genomic complex: As used herein, the term "genomic complex" is a complex that brings .. together two genomic sequence elements that are spaced apart from one another on one or more chromosomes, via interactions between and among a plurality of protein and/or other components (potentially including, the genomic sequence elements). In some embodiments, the genomic sequence elements are anchor sequences to which one or more protein components of the complex binds. In some embodiments, a genomic complex may comprise an anchor sequence-mediated conjunction. In some embodiments, a genomic sequence element may be or comprise a CTCF binding motif, a promoter and/or an enhancer. In some embodiments, a genomic sequence element includes at least one or both of a promoter and/or regulatory site (e.g., an enhancer). In some embodiments, complex formation is nucleated at the genomic sequence element(s) and/or by binding of one or more of the protein component(s) to the genomic sequence element(s). As will be understood by those skilled in the art, in some embodiments, co-localization (e.g., conjunction) of the genomic sites via formation of the complex alters DNA topology at or near the genomic sequence element(s), including, in some embodiments, between them. In some embodiments, a genomic complex comprises an anchor sequence-mediated conjunction, which comprises one or more loops. In some embodiments, a genomic complex as described herein is nucleated by a nucleating polypeptide such as, for example, CTCF
and/or Cohesin. In some embodiments, a genomic complex as described herein may include, for example, one or more of CTCF, Cohesin, non-coding RNA (e.g., eRNA), transcriptional machinery proteins (e.g., RNA polymerase, one or more transcription factors, for example selected from the group consisting of TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIH, etc.), transcriptional regulators (e.g., Mediator, P300, enhancer-binding proteins, repressor-binding proteins, histone modifiers, etc.), etc. In some embodiments, a genomic complex as described herein includes one or more polypeptide components and/or one or more nucleic acid components (e.g., one or more RNA components), which may, in some embodiments, be interacting with one another and/or with one or more genomic sequence elements (e.g., anchor sequences, promoter sequences, regulatory sequences (e.g., enhancer sequences)) so as to constrain a stretch of genomic DNA
into a topological configuration (e.g., a loop) that it does not adopt when the complex is not formed.
Nucleic acid: As used herein, in its broadest sense, the term "nucleic acid"
refers to any compound and/or substance that is or can be incorporated into an oligonucleotide chain. In some embodiments, a nucleic acid is a compound and/or substance that is or can be incorporated into an oligonucleotide chain via a phosphodiester linkage. As will be clear from context, in some embodiments, "nucleic acid" refers to an individual nucleic acid residue (e.g., a nucleotide and/or nucleoside); in some embodiments, "nucleic acid" refers to an oligonucleotide chain comprising individual nucleic acid residues. In some embodiments, a "nucleic acid" is or comprises RNA; in some embodiments, a "nucleic acid" is or comprises DNA. In some embodiments, a nucleic acid is, comprises, or consists of one or more natural nucleic acid residues. In some embodiments, a nucleic acid is, comprises, or consists of one or more nucleic acid analogs. In some embodiments, a nucleic acid analog differs from a nucleic acid in that it does not utilize a phosphodiester backbone. For example, in some embodiments, a nucleic acid is, comprises, or consists of one or more "peptide nucleic acids", which are known in the art and have peptide bonds instead of phosphodiester bonds in the backbone, are considered within the scope of the present disclosure. Alternatively, or additionally, in some embodiments, a nucleic acid has one or more phosphorothioate and/or 5'-N-phosphoramidite linkages rather than phosphodiester bonds. In some embodiments, a nucleic acid is, comprises, or consists of one or more natural nucleosides (e.g., adenosine, thymidine, guanosine, cytidine, uridine, deoxyadenosine, deoxythymidine, deoxy guanosine, and deoxycytidine). In some embodiments, a nucleic acid is, comprises, or consists of one or more nucleoside analogs (e.g., 2-aminoadenosine, 2-thiothymidine, inosine, pyrrolo-pyrimidine, 3 -methyl adenosine, 5-methylcytidine, C-5 propynyl-cytidine, C-5 propynyl-uridine, 2-aminoadenosine, C5-bromouridine, C5-fluorouridine, C5-iodouridine, C5-propynyl-uridine, C5 -propynyl-cytidine, C5-methylcytidine, 2-aminoadenosine, 7-deazaadenosine, 7-deazaguanosine, 8-oxoadenosine, 8-oxoguanosine, 0(6)-methylguanine, 2-thiocytidine, methylated bases, intercalated bases, and combinations thereof). In some embodiments, a nucleic acid comprises one or more modified sugars (e.g., 2'-fluororibose, ribose, 2'-deoxyribose, arabinose, and hexose) as compared with those in natural nucleic acids. In some embodiments, a nucleic acid has a nucleotide sequence that encodes a functional gene product such as an RNA or protein. In some embodiments, a nucleic acid includes one or more introns. In some embodiments, nucleic acids are prepared by one or more of isolation from a natural source, enzymatic 5 synthesis by polymerization based on a complementary template (in vivo or in vitro), reproduction in a recombinant cell or system, and chemical synthesis. In some embodiments, a nucleic acid is at least 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 20, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 600, 700, 800, 900, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000 or more residues long. In some 10 embodiments, a nucleic acid is partly or wholly single stranded; in some embodiments, a nucleic acid is partly or wholly double stranded. In some embodiments a nucleic acid has a nucleotide sequence comprising at least one element that encodes, or is the complement of a sequence that encodes, a polypeptide. In some embodiments, a nucleic acid has enzymatic activity.
Operably linked: As used herein, the phrase "operably linked" refers to a juxtaposition wherein 15 the components described are in a relationship permitting them to function in their intended manner. A
transcription control element "operably linked" to a functional element, e.g., gene, is associated in such a way that expression and/or activity of the functional element, e.g., gene, is achieved under conditions compatible with the transcription control element. In some embodiments, "operably linked" transcription control elements are contiguous (e.g., covalently linked) with coding elements, e.g., genes, of interest; in 20 some embodiments, operably linked transcription control elements act in trans to or otherwise at a distance from the functional element, e.g., gene, of interest. In some embodiments, operably linked means two nucleic acid sequences are comprised on the same nucleic acid molecule. In a further embodiment, operably linked may further mean that the two nucleic acid sequences are proximal to one another on the same nucleic acid molecule, e.g., within 1000, 500, 100, 50, or 10 base pairs of each other 25 or directly adjacent to each other.
Peptide, Polyp eptide, Protein: As used herein, the terms "peptide,"
"polypeptide," and "protein"
refer to a compound comprised of amino acid residues covalently linked by peptide bonds, or by means other than peptide bonds. A protein or peptide must contain at least two amino acids, and no limitation is 30 placed on the maximum number of amino acids that can comprise a protein's or peptide's sequence.
Polypeptides include any peptide or protein comprising two or more amino acids joined to each other by peptide bonds or by means other than peptide bonds. As used herein, the term refers to both short chains, which also commonly are referred to in the art as peptides, oligopeptides, and oligomers, for example, and to longer chains, which generally are referred to in the art as proteins, of which there are many types.
Repressor domain: As used herein, the term "repressor domain" refers to a domain with one or more functionalities that decrease expression of a target gene in a cell when appropriately localized in the nucleus of a cell. In some embodiments, a repressor domain is or comprises a polypeptide. In some embodiments, a repressor domain is or comprises a polypeptide and a nucleic acid. A functionality associated with a repressor domain may directly affect expression of a target gene, e.g., blocking recruitment of a transcription factor that would stimulate expression of the gene. A functionality associated with a repressor domain may indirectly affect expression of a target gene, e.g., introducing epigenetic modifications or recruiting other factors that introduce epigenetic modifications that induce a change in chromosomal topology that inhibits expression of a target gene.
Specific binding: As used herein, the term "specific binding" refers to an ability to discriminate between possible binding partners in the environment in which binding is to occur. In some embodiments, a binding agent that interacts with one particular target when other potential targets are present is said to "bind specifically" to the target with which it interacts.
In some embodiments, specific binding is assessed by detecting or determining degree of association between the binding agent and its .. partner; in some embodiments, specific binding is assessed by detecting or determining degree of dissociation of a binding agent-partner complex. In some embodiments, specific binding is assessed by detecting or determining ability of the binding agent to compete with an alternative interaction between its partner and another entity. In some embodiments, specific binding is assessed by performing such detections or determinations across a range of concentrations.
Substantially: As used herein, the term "substantially" refers to the qualitative condition of exhibiting total or near-total extent or degree of a characteristic or property of interest. One of ordinary skill in the art will understand that biological and chemical phenomena rarely, if ever, go to completion and/or proceed to completeness or achieve or avoid an absolute result. The term "substantially" may therefore be used in some embodiments herein to capture potential lack of completeness inherent in many biological and chemical phenomena.
Symptoms are reduced: As used herein, the phrase "symptoms are reduced" may be used when one or more symptoms of a particular disease, disorder or condition is reduced in magnitude (e.g., intensity, severity, etc.) and/or frequency. In some embodiments, a delay in the onset of a particular symptom is considered one form of reducing the frequency of that symptom.
Target: An agent or entity is considered to "target" another agent or entity, in accordance with the present disclosure, if it binds specifically to the targeted agent or entity under conditions in which they come into contact with one another. In some embodiments, for example, an antibody (or antigen-binding fragment thereof) targets its cognate epitope or antigen. In some embodiments, a nucleic acid having a particular sequence targets a nucleic acid of substantially complementary sequence.
Target gene: As used herein, the term "target gene" means a gene that is targeted for modulation, e.g., of expression. In some embodiments, a target gene is part of a targeted genomic complex (e.g., a gene that has at least part of its genomic sequence as part of a target genomic complex, e.g. inside an anchor sequence-mediated conjunction), which genomic complex is targeted by one or more modulating agents as described herein. In some embodiments, modulation comprises inhibition of expression of the target gene. In some embodiments, a target gene is modulated by contacting the target gene or a transcription control element operably linked to the target gene with an expression repression system, e.g., expression repressor(s), described herein. In some embodiments, a target gene is aberrantly expressed (e.g., over-expressed) in a cell, e.g., a cell in a subject (e.g., patient).
DNA-targeting moiety: As used herein, in the context of expression repressors, the term "DNA-targeting moiety" means a domain that specifically binds to a DNA sequence (e.g., a DNA sequence associated with a target gene, or a transcription control element operably linked to a target gene).
Therapeutic agent: As used herein, the phrase "therapeutic agent" refers to an agent that, when administered to a subject, has a therapeutic effect and/or elicits a desired biological and/or pharmacological effect. In some embodiments, a therapeutic agent is any substance that can be used to alleviate, ameliorate, relieve, inhibit, prevent, delay onset of, reduce severity of, and/or reduce incidence of one or more symptoms or features of a disease, disorder, and/or condition.
In some embodiments, a therapeutic agent comprises an expression repression system, e.g., an expression repressor, described herein. In some embodiments, a therapeutic agent comprises a nucleic acid encoding an expression repression system, e.g., an expression repressor, described herein. In some embodiments, a therapeutic agent comprises a pharmaceutical composition described herein.
Therapeutically effective amount: As used herein, the term "therapeutically effective amount"
means an amount of a substance (e.g., a therapeutic agent, composition, and/or formulation) that elicits a desired biological response when administered as part of a therapeutic regimen. In some embodiments, a therapeutically effective amount of a substance is an amount that is sufficient, when administered to a subject suffering from or susceptible to a disease, disorder, and/or condition, to treat, diagnose, prevent, and/or delay the onset of the disease, disorder, and/or condition. As will be appreciated by those of ordinary skill in this art, an effective amount of a substance may vary depending on such factors as desired biological endpoint(s), substance to be delivered, target cell(s) or tissue(s), etc. For example, in some embodiments, an effective amount of compound in a formulation to treat a disease, disorder, and/or condition is an amount that alleviates, ameliorates, relieves, inhibits, prevents, delays onset of, reduces severity of and/or reduces incidence of one or more symptoms or features of the disease, disorder, and/or condition. In some embodiments, a therapeutically effective amount is administered in a single dose; in some embodiments, multiple unit doses are required to deliver a therapeutically effective amount.
BRIEF DESCRIPTION OF THE DRAWING
The following detailed description of the embodiments of the invention will be better understood when read in conjunction with the appended drawings. For the purpose of illustrating the invention, there .. are shown in the drawings embodiments, which are presently exemplified. It should be understood, however, that the invention is not limited to the precise arrangement and instrumentalities of the embodiments shown in the drawings.
Figure 1 shows a Genome Browser view showing key features of the region near the transcription start site of I32M. RNAseq (top level) shows mRNA expression from the I32M gene. A
previously described positive control (Cas9 targeting, GD-21547) is shown in green. Blue guides (GD-28228 and GD-28229) were used to target Sp-dCas9-KRAB and orange guides (GD-28171, GD-28172 and GD-28173) were used to target Sa-dCas9-MQ1 to the CpG Island in the I32M
promoter region (second level from the top). Guides were chosen based on hypomethylation of the promoter region (depicted in the CpG Methylation track, bottom level), where methylation runs from 0 to 1 (0 to 100%) and where higher methylation is depicted by red bars. Each bar represents a CpG site.
Figure 2 shows a graph of percent change in I3-2-microglobulin expression (mRNA) in HepG2 cells with treatment with a variety of expression repressors or controls. I32M
mRNA levels shown are relative to the untreated control (gray). Points show biological replicates.
Bars show the average and error bars indicate standard deviations. The guide ID number is shown on the x-axis.
Samples treated with dCas9 are shown in magenta; dCas9-KRAB in green; sa-dCas9-MQ1 in light blue; a combination of dCas9-KRAB and sa-dCas9-MQ1 in dark blue. A 132M-knockout positive control with Cas9 is shown in orange.
Figure 3 shows a graph of percent change in I3-2-microglobulin mRNA with treatment with a variety of expression repressors or controls after 72 hours (left), and a graph of flow cytometry data of 13-2-microglobulin protein levels with treatment with a variety of expression repressors or controls after 72 hours (right). (Left) Treatment with dCas9-KRAB (green) and Sa-dCas9-MQ1 (light blue) effectors, separately and together (dark blue) results in a decrease in I32M mRNA
relative to untreated cells (grey) and cells treated with dCas9 (magenta). (Right) Flow cytometry revealed populations with decreased I32M
immunofluorescence in cells treated with dCas9-KRAB and Sa-dCas9-MQ1 relative to untreated cells .. and cells treated with dCas9.
Figure 4 shows a graph of the fold change in I3-2-microglobulin mRNA with treatment with a variety of expression repressors or controls over a 22-day period. Over a 22-day period, non-transfected (blue line) and dCas9 transfected (red line) cells don't change I32M
expression, whereas dCas9-KRAB
(green line) transfected cells have I32M repressed in the initial days post-transfection, with maximum effect on day 2, but the repression is lost after day 5. SadCas9-MQ1 (purple line) transfected cells show significant repression (up to 85% by day 4) and the repression is retained and then slowly lost as seen by line remaining just under the dotted line in the figure. dCas9-KRAB + SadCas9-MQ1 combined transfection (orange line) did not give any added repression than what is already observed for SadCas9 alone (purple line).
Figure 5 shows a graph of the fold change in I3-2-microglobulin mRNA with treatment with a variety of expression repressors or controls over an 18-day period. Over an 18-day period untreated cells do not change I32M expression, whereas cells transfected with dCas9-MQ1, dCas9-DNMT3a/3L (m), dCas9-DNMT3a/3L (hs), dCas9-DNMT1 and dCas9-DNMT3B, show significant I32M
repression post-.. transfection.
Figure 6 shows a graph of the fold change in MYC expression in K-562 cells when treated with different expression repressors or expression repression systems that target the CTCF site located upstream of MYC gene. The data showed that, at least, cells treated with dCas9-DNMT3a/3L (h), dCas9-KRAB, FOG1-dCas9-FOG1, G9A-dCas9 + EZH2-dCas9, dCas9-MQ1 + EZH2-dCas9-KRAB, dCas9-LSD1+G9A-dCas9, dCas9-MQ1+dCas9-HDAC8, and dCas9-MQ1+G9A-dCas9-KRAB showed repression of MYC expression at 48 hour and/or 72-hour time point.
Figure 7 shows a graph of the fold change in I32M expression in K-562 cells when treated with different expression repressors or expression repression systems that target a region upstream of the I32M
promoter. The data showed that, at least, cells treated with dCas9-HDAC8, dCas9-LSD1, dCas9-MQ1, FOG1-dCas9- FOG1, G9A-dCas9-KRAB, dCas9-DNMT3a/3L (h), G9A-dCas9, EZH2-dCas9-KRAB, dCas9-KRAB, EZH2-dCas9-KRAB + dCas9-HDAC8, dCas9-LSD1 + EZH2-dCas9-KRAB, dCas9-LSD1 + dCas9-HDAC8, dCas9-KRAB + FOG1-dCas9- FOG1, dCas9-MQ1 + G9A-dCas9-KRAB, dCas9 + EZH2-dCas9, dCas9-MQ1 + EZH2-dCas9-KRAB, and dCas9-MQ1 + dCas9-HDAC8 showed repression of I32M expression at 48 hour, 72 hour and/or 144 hour time point.
Figure 8 shows a graph of the fold change in HSPA1B expression in K-562 cells when treated with different expression repressors or expression repression systems that target a region downstream of the HSPA1B promoter. The data showed that, at least, cells treated with dCas9-HDAC8, EZH2-dCas9, dCas9-MQ1, dCas9-DNMT1, dCas9-DNMT3a/3L (h), dCas9-DNMT3a/3L (m), G9A-dCas9-KRAB, FOG1-dCas9-FOG1, G9A-dCas9, dCas9-KRAB, G9A-dCas9 + dCas9-HDAC8, dCas9-LSD1 +
dCas9-KRAB, dCas9-LSD1+EZH2-dCas9, G9A-dCas9+EZH2-dCas9, dCas9-LSD1 + EZH2-dCas9-KRAB, EZH2-dCas9 +dCas9-HDAC8, dCas9-LSD1+dCas9-HDAC8, dCas9-MQ1 + EZH2-dCas9-KRAB, dCas9-MQ1+dCas9-HDAC8, G9A-dCas9-KRAB+EZH2-dCas9-KRAB, dCas9-LSD1+G9A-dCas9 and dCas9-MQ1+G9A-dCas9-KRAB showed repression of HSPA1B expression at 48 hour, 72 hour and/or 144 hour time point.
Figure 9 shows a graph of the fold change in GATA1 expression in K-562 cells when treated with different expression repressors or expression repression systems. The data showed that, at least, cells treated with dCas9-HDAC8, dCas9-LSD1+dCas9-HDAC8, dCas9-MQ1+dCas9-HDAC8, and dCas9+dCas9-HDAC8 showed repression of GATA1 expression at 48-hour, 72 hour and/or 144 hour 5 time point.
DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS
10 The present disclosure provides technologies for modulating, e.g., decreasing, expression of a target gene in cell, e.g., in a subject or patient, through the use of an expression repressor or an expression repression system. In some embodiments, an expression repressor comprises a DNA binding moiety and at least one repressor domain. In some embodiments, an expression repressor comprises two repressor domains. In some embodiments an expression repressor comprises two identical repressor domains. In 15 some embodiments an expression repressor comprises two non-identical repressor domains.
In some embodiments, an expression repression system comprises two or more expression repressors, each comprising a DNA-targeting moiety and at least one repressor domain. In some embodiments, the DNA-targeting moieties target two or more different DNA sequences (e.g., each expression repressor may target a different DNA sequence). Without wishing to be bound by theory, the use of an expression 20 repression system comprising expression repressors targeting two or more different DNA sequences may be advantageous over similar systems targeting a single DNA sequence because individual expression repressors will not compete with one another or compete less with one another for binding to their target DNA sequences, thereby achieving superior localization of the various functionalities of the repressor domains. In some embodiments, it can be advantageous to use two different DNA¨targeting moieties to 25 precisely target two different repressors to two different defined locations, e.g., a first expression repressor to a first location upstream of the transcription start site and a second expression repressor to a second location comprising the transcription start site. In some embodiments, the expression repressors comprise two or more different repressor domains (e.g., each expression repressor comprises a different repressor domain from each other expression repressor). Without wishing to be bound by theory, the use 30 of an expression repression system comprising expression repressors comprising two or more repressor domains may be advantageous over similar systems comprising a single repressor domain due to synergistic effects on expression of a target gene associated with the concerted action of the two or more repressor domains.
Expression Repressors Expression repressors of the present disclosure may comprise a DNA-targeting moiety and at least one repressor domain. In some embodiments, an expression repressor may comprise 1, 2, 3, 4, 5, 6, or more repressor domains. In some embodiments, an expression repressor targets two or more different DNA sequences (e.g., a Pt and 2nd, 3rd, 4th, 5th, 6th, 7th, 8th, V,--sth, 10th, 11th, 12th, and/or further DNA
sequence, and optionally no more than a 20th, 19th, 18th, 17th, 16th, 15th, 14th, 13th, 12th, llth, 10th, 9th, 8th, 6th, 5th, 4th, 3rd, or 2nd DNA sequence). In some embodiments, an expression repressor comprises a DNA-targeting moiety and a plurality of repressor domains (e.g., 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or more repressor domains (and optionally, less than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, or 2 repressor domains)) each of which may be the same or different from another of the more than one repressor domains.
In some embodiments, an expression repressor comprises a first repressor domain and a second repressor domain, wherein the first repressor domain is not identical to the second repressor domain. In some embodiments, an expression repressor comprises a first repressor domain and a second repressor domain, wherein the first repressor domain is identical to the second repressor domain. In some embodiments, the DNA-targeting moiety is situated between the first repressor domain and the second repressor domain.
An expression repressor may comprise a plurality of repressor domains, where each repressor domain comprises a different functionality than the other repressor domains.
For example, an expression repressor may comprise two repressor domains, where the first repressor domain comprises DNA
methylase functionality and the second repressor domain comprises a transcriptional repressor functionality. In some embodiments, an expression repressor comprises repressor domains whose functionalities are complementary to one another with regard to decreasing expression of a target gene, where the functionalities together enable inhibition of expression and, optionally, do not inhibit or negligibly inhibit expression when present individually. In some embodiments, an expression repressor comprises a plurality of repressor domains, wherein each repressor domain complements other repressor domains, each repressor domain decreases expression of a target gene.
In some embodiments, an expression repressor comprises a combination of repressor domains whose functionalities synergize with one another with regard to decreasing expression of a target gene.
Without wishing to be bound by theory, in some embodiments, epigenetic modifications to a genomic locus are cumulative, in that multiple transcription activating epigenetic markers (e.g., multiple different types of epigenetic markers and/or more extensive marking of a given type) individually together inhibit expression more effectively than individual modifications alone (e.g., producing a greater decrease in expression and/or a longer-lasting decrease in expression). In some embodiments, an expression repressor comprises a plurality of repressor domains, wherein each repressor domain synergizes with other repressor domains, e.g., each repressor domain decreases expression of a target gene. In some embodiments, an expression repressor (comprising a plurality of repressor domains which synergize with one another) is more effective at inhibiting expression of a target gene than an expression repressor comprising an individual repressor domain. In some embodiments, an expression repressor comprising said plurality of repressor domains is at least 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.55x, 1.6x, 1.65x, 1.7x, 1.75x, 1.8x, 1.85x, 1.9x, 1.95x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 30x, 40x, 50x, 60x, 70x, 80x, 90x, or 100x as effective at decreasing expression of a target gene than an expression repressor comprising an individual repressor domain.
In some embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain that are covalently linked, e.g., by a peptide bond. In some embodiments, the DNA-targeting moiety and the repressor domain are situated on the same polypeptide chain, e.g., connected by one or more peptide bonds and/or a linker. In some embodiments, the expression repressor is or comprises .. a fusion molecule, e.g., comprising the DNA-targeting moiety and the repressor domain linked by a peptide bond and/or a linker. In some embodiments, an expression repressor comprises a targeting moiety and a plurality of effector moieties, wherein the targeting moiety and the plurality of effector moieties are covalently linked, e.g., by peptide bonds (e.g., the targeting moiety and plurality of effector moieties are all connected by a series of covalent bonds, although each individual moiety may not share a covalent .. bond with every other effector moiety). In some embodiments, the expression repressor comprises a DNA-targeting moiety that is linked to the N-terminal of a repressor domain on the same polypeptide chain. In some embodiments, the expression repressor comprises a DNA-targeting moiety that is linked to the C-terminal of a repressor domain on the same polypeptide chain. In some embodiments, the expression repressor comprises a DNA-targeting moiety that is linked to the C-terminal of a first repressor domain and is linked to the N-terminal of a second repressor domain on the same polypeptide chain. In some embodiments, the expression repressor comprises a DNA-targeting moiety that is disposed N-terminal of a repressor domain on the same polypeptide chain. In some embodiments, the expression repressor comprises a DNA-targeting moiety that is disposed C-terminal of a repressor domain on the same polypeptide chain. In some embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain that are covalently linked by a non-peptide bond. In some embodiments, a DNA-targeting moiety is conjugated to a repressor domain by a non-peptide bond. In some embodiments, an expression repressor comprises a DNA-targeting moiety and a plurality of repressor domains, wherein the DNA-targeting moiety and the plurality of repressor domains are covalently linked, e.g., by peptide bonds (e.g., the DNA-targeting moiety and plurality of repressor domains are all connected by a series of covalent bonds, although each individual domain or moiety may not share a covalent bond with every other domain or moiety).
In other embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain that are not covalently linked, e.g., that are non-covalently associated with one another.
In some embodiments, an expression repressor comprises a DNA-targeting moiety that non-covalently binds to a repressor domain or vice versa. In some embodiments, an expression repressor comprises a DNA-targeting moiety and a plurality of repressor domains, wherein the DNA-targeting moiety and at least one repressor domain are not covalently linked, e.g., are non-covalently associated with one another, and wherein the DNA-targeting moiety and at least one other repressor domain are covalently linked, e.g., by a peptide bond.
In general, an expression repressor as described herein binds (e.g., via a DNA-targeting moiety) a genomic sequence element proximal to and/or operably linked to a target gene.
In some embodiments, binding of the expression repressor to the genomic sequence element modulates (e.g., decreases) expression of the target gene. For example, binding of an expression repressor comprising a repressor domain that recruits or inhibits recruitment of components of the transcription machinery to the genomic sequence element may modulate (e.g., decrease) expression of the target gene.
As a further example, binding of an expression repressor comprising a repressor domain with an enzymatic activity (e.g., an epigenetic modifying moiety) may modulate (e.g., decrease) expression of the target gene) through the localized enzymatic activity of the repressor domain. As a further example, both binding of an expression repressor to a genomic sequence element and the localized enzymatic activity of an expression repressor may contribute to the resulting modulation (e.g., decrease) in expression of the target gene.
In some embodiments, an expression repressor comprises a repressor domain wherein the repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, an expression repressor comprises a first repressor domain wherein the repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof and a second repressor domain wherein the repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain wherein the C-terminal end of the repressor domain, e.g., a repressor domain chosen from EZH1, EZH2, G9A, SUV39H1, FOG1, SETDB1, or SETDB2, and the N-terminal end of the DNA-5 targeting moiety are covalently linked. In some embodiments, an expression repressor comprises a DNA-targeting moiety and a repressor domain wherein the N-terminal end of the repressor domain, e.g., a repressor domain chosen from LSD1, HDAC8, MQ1, DNMT1, DNMT3a/3L, FOG1, or KRAB, and the C-terminal end of the DNA-targeting moiety are covalently linked. In some embodiments, an expression repressor comprises a DNA-targeting moiety, a first repressor domain and second repressor domain, 10 wherein, the C-terminal end of the first repressor domain, e.g., a repressor domain chosen from EZH1, EZH2, G9A, SUV39H1, FOG1, SETDB1, or SETDB2, and the N-terminal end of the DNA-targeting moiety are covalently linked and the C-terminal end of the DNA-targeting moiety and the N-terminal end of the second repressor domain, e.g., a repressor domain chosen from LSD1, HDAC8, MQ1, DNMT1, DNMT3a/3L, FOG1, or KRAB are covalently linked.
15 In some embodiments, an expression repressor as disclosed herein is present in a composition, pharmaceutical composition, or mixture.
While several of the embodiments herein describe systems comprising a plurality of expression repressors, it is understood that the present application also provides the expression repressors individually, e.g., for use as a single agent, or for use in a combination with a generic second therapy that 20 need not be specified herein.
In some embodiments, an expression repressor described herein is part of an expression repression system, e.g., as described below.
Expression Repression Systems 25 Expression repression systems of the present disclosure may comprise two or more expression repressors. In some embodiments, an expression repression system comprises 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or more expression repressors (and optionally no more than 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, or 2). In some embodiments, an expression repression system targets two or more different DNA sequences (e.g., a 1st and 2nd, 3rd, 4th, 5th, 6th, 7th, 8th, V,--sth, 10th, 11th 12th, and/or further DNA sequence, and optionally 30 no more than a 20th, 19th, 18th, 17th, 16th, 15th, 14th, 13th, 12th, llth, 10th, 9th, 8th, 6th, 5th, 4th, 3rd, or 2nd DNA
sequence). In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each member of the plurality of expression repressors does not detectably bind, e.g., does not bind, to another member of the plurality of expression repressors. In some embodiments, an expression repression system comprises a first expression repressor and a second expression repressor, wherein the first expression repressor does not detectably bind, e.g., does not bind, to the second expression repressor.
In some embodiments, an expression repression system of the present disclosure comprises two or more expression repressors, wherein the expression repressors are present together in a composition, pharmaceutical composition, or mixture. In some embodiments, an expression repression system of the present disclosure comprises two or more expression repressors, wherein one or more expression repressors is not admixed with at least one other expression repressor. For example, an expression repression system may comprise a first expression repressor and a second expression repressor, wherein the presence of the first expression repressor in the nucleus of a cell does not overlap with the presence of the second expression repressor in the nucleus of the same cell, wherein the expression repression system achieves a decrease in expression of a target gene via the non-overlapping presences of the first and second expression repressors.
In some embodiments, the expression repressors of an expression repression system each comprise a different DNA-targeting moiety (e.g., the first, second, third, or further expression repressors each comprise different DNA-targeting moieties from one another). For example, an expression repression system may comprise a first expression repressor and a second expression repressor wherein the first expression repressor comprises a first DNA-targeting moiety (e.g., a Cas9 molecule, TAL
effector molecule, or Zn Finger domain), and the second expression repressor comprises a second DNA-targeting moiety (e.g., a Cas9 molecule, TAL effector molecule, or Zn Finger domain) different from the first DNA-targeting moiety. In some embodiments, different can mean comprising distinct types of DNA-targeting moiety, e.g., the first DNA-targeting moiety comprises a Cas9 molecule, and the second DNA-targeting moiety comprises a TAL effector molecule. In other embodiments, different can mean comprising distinct variants of the same type of DNA-targeting moiety, e.g., the first DNA-targeting moiety comprises a first Cas9 molecule (e.g., from a first species) and the second DNA-targeting moiety comprises a second Cas9 molecule (e.g., from a second species). In an embodiment, when an expression repression system comprises two or more DNA-targeting moieties of the same type, e.g., two or more Cas9 molecules, the DNA-targeting moieties specifically bind two or more different DNA sequences. For example, in an expression repression system comprising two or more Cas9 molecules, the two or more Cas9 molecules may be chosen or altered such that they only appreciably bind the gRNA corresponding to their target DNA sequence (e.g., and do not appreciably bind the gRNA
corresponding to the target of another Cas9 molecule). In a further example, in an expression repression system comprising two or more TAL effector molecules, the two or more TAL effector molecules may be chosen or altered such that they only appreciably bind to their target DNA sequence (e.g., and do not appreciably bind the target DNA
sequence of another TAL effector molecule).
In some embodiments, an expression repression system comprises three or more expression repressors and two or more expression repressors comprise the same DNA-targeting moiety. For example, an expression repression system may comprise three expression repressors, wherein the first and second expression repressors both comprise a first DNA-targeting moiety and the third expression repressor .. comprises a second different DNA-targeting moiety. For a further example, an expression repression system may comprise four expression repressors, wherein the first and second expression repressors both comprise a first DNA-targeting moiety and the third and fourth expression repressors comprises a second different DNA-targeting moiety. For a further example, an expression repression system may comprise five expression repressors, wherein the first and second expression repressors both comprise a first DNA-targeting moiety, the third and fourth expression repressors both comprise a second different DNA-targeting moiety, and the fifth expression repressor comprises a third different DNA-targeting moiety. As described above, different can mean comprising different types of DNA-targeting moieties or comprising distinct variants of the same type of DNA-targeting moiety.
In some embodiments, the expression repressors of an expression repression system each bind to a different DNA sequence (e.g., the first, second, third, or further expression repressors each bind DNA
sequences that are different from one another). For example, an expression repression system may comprise a first expression repressor and a second expression repressor wherein the first expression repressor binds to a first DNA sequence, and the second expression repressor binds to a second DNA
sequence. In some embodiments, different can mean that: there is at least one position that is not identical between the DNA sequence bound by one expression repressor and the DNA
sequence bound by another expression repressor, or that there is at least one position present in the DNA sequence bound by one expression repressor that is not present in the DNA sequence bound by another expression repressor. For example, a first expression repressor may bind to a first exemplary DNA
sequence 5'-ATGATTGGATTTA-3' (SEQ ID NO: 97), and a second expression repressor may bind to a second exemplary DNA sequence 5'- TGATTGGATTTAG-3' (SEQ ID NO: 98); in said example, the first and second exemplary DNA sequences are different. For a further example, a first expression repressor may bind to a first exemplary DNA sequence 5'-ATGATTgGATTTA-3' (SEQ ID NO: 99), and a second expression repressor may bind to a second exemplary DNA sequence 5'-ATGATTcGATTTA-3' (SEQ
ID NO: 100); in said example, the first and second exemplary DNA sequences are different. In some embodiments, the first DNA sequence may be situated on a first genomic DNA
strand and the second DNA sequence may be situated on a second genomic DNA strand. In some embodiments, the first DNA
sequence may be situated on the same genomic DNA strand as the second DNA
sequence.
In some embodiments, an expression repression system comprises three or more expression repressors and two or more expression repressors bind the same DNA sequence.
For example, an expression repression system may comprise three expression repressors, wherein the first and second expression repressors both bind a first DNA sequence, and the third expression repressor binds a second different DNA sequence. For a further example, an expression repression system may comprise four expression repressors, wherein the first and second expression repressors both bind a first DNA sequence and the third and fourth expression repressors both bind a second DNA
sequence. For a further example, an expression repression system may comprise five expression repressors, wherein the first and second expression repressors both bind a first DNA sequence, the third and fourth expression repressors both bind a second DNA sequence, and the fifth expression repressor binds a third DNA sequence. As described above, different can mean that there is at least one position that is not identical between the DNA sequence bound by one expression repressor and the DNA sequence bound by another expression repressor, or that there is at least one position present in the DNA sequence bound by one expression repressor that is not present in the DNA sequence bound by another expression repressor.
In some embodiments, an expression repression system comprises two or more (e.g., two) expression repressors and a plurality (e.g., two) of the expression repressors comprise DNA-targeting moieties that bind to different DNA sequences. In such embodiments, a first DNA-targeting moiety may bind to a first DNA sequence and a second DNA-targeting moiety may bind to a second DNA sequence, wherein the first and the second DNA sequences are different and do not overlap. In some such embodiments, the first DNA sequence is separated from the second DNA sequence by at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 base pairs (and optionally, no more than 500, 400, 300, 200, 100, 95, 90, 85, 80, 75, 70, 65, 60, 55, or 50 base pairs). In some such embodiments, the first DNA sequence is separated from the second DNA sequence by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 base pairs (and optionally, no base pairs, e.g., the first and second sequence are directly adjacent one another).
In some embodiments, the expression repressors of an expression repression system each comprise a different repressor domain (e.g., the first, second, third, or further expression repressors each comprise a different repressor domain from one another). For example, an expression repression system may comprise a first expression repressor and a second expression repressor wherein the first expression repressor comprises a first repressor domain (e.g., comprising a histone methyltransferase or functional fragment thereof), and the second expression repressor comprises a second repressor domain (e.g., comprising a DNA methyltransferase or functional fragment thereof) different from the first repressor domain. In some embodiments, different can mean comprising distinct types of repressor domain, e.g., the first repressor domain comprises a histone methyltransferase and the second repressor domain comprises a DNA methyltransferase, or the first repressor domain comprises a histone methyltransferase and the second repressor domain comprises a small molecule inhibitor of an enzyme. In other embodiments, different can mean comprising distinct variants of the same type of repressor domain, e.g., the first repressor domain comprises a first histone methyltransferase (e.g., having a first site specificity or amino acid sequence) and the second repressor domain comprises a second histone methyltransferase (e.g., .. having a second site specificity or amino acid sequence).
In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor domain, wherein the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, .. KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a .. functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, .. SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor .. domain, wherein the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, .. DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, 5 RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the third repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, 10 SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, an expression repression system comprises: (i) a first expression repressor comprising a first repressor domain and a third repressor domain, and (ii) a second expression repressor 15 comprising a second repressor domain and optionally a fourth repressor domain, wherein the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, 20 HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, 25 SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, 30 FOG1, SUZ12, or a functional variant or fragment thereof, the third repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B
(i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof and the fourth repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, .. DNMT3L, DNMT3a/3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
In some embodiments, the first repressor domain comprises a histone methyltransferase activity (e.g., SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, or a functional variant or fragment of any thereof, e.g., a SET domain of any thereof) and the second repressor domain comprises a histone demethylase activity (e.g., KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, or a functional variant or fragment of any thereof). In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a histone deacetylase activity (e.g., HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof). In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a DNA
methyltransferase activity (e.g., MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, or a functional variant or fragment of any thereof). In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a DNA
demethylase activity. In some embodiments the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a transcription repressor activity (e.g., KRAB, MeCP2, HP1, RBBP4, .. REST, FOG1, SUZ12, or a functional variant or fragment of any thereof). In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises a different histone methyltransferase activity. In some embodiments, the first repressor domain comprises a histone methyltransferase activity and the second repressor domain comprises the same histone methyltransferase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a histone deacetylase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a DNA methyltransferase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a DNA
demethylase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a transcription repressor activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises a different histone demethylase activity. In some embodiments, the first repressor domain comprises a histone demethylase activity and the second repressor domain comprises the same histone demethylase activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a DNA
methyltransferase activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a DNA demethylase activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a transcription repressor activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises a different histone deacetylase activity. In some embodiments, the first repressor domain comprises a histone deacetylase activity and the second repressor domain comprises the same histone deacetylase activity. In some embodiments, the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a DNA demethylase activity. In some embodiments, the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises a transcription repressor activity. In some embodiments, the first repressor domain comprises a DNA
methyltransferase activity and the second repressor domain comprises a different DNA methyltransferase activity. In some embodiments, the first repressor domain comprises a DNA methyltransferase activity and the second repressor domain comprises the same DNA methyltransferase activity. In some embodiments, the first repressor domain comprises a DNA demethylase activity and the second repressor domain comprises a transcription repressor activity. In some embodiments, the first repressor domain comprises a DNA
demethylase activity and the second repressor domain comprises a different DNA
demethylase activity. In some embodiments, the first repressor domain comprises a DNA demethylase activity and the second .. repressor domain comprises the same DNA demethylase activity. In some embodiments, the first repressor domain comprises a transcription repressor activity and the second repressor domain comprises a different transcription repressor activity. In some embodiments, the first repressor domain comprises a transcription repressor activity and the second repressor domain comprises the same transcription repressor activity.
In some embodiments, an expression repression system comprises three or more expression repressors and two or more expression repressors comprise the same DNA-targeting moiety. For example, an expression repression system may comprise three expression repressors, wherein the first and second expression repressors both comprise a first repressor domain and the third expression repressor comprises a second different repressor domain. For a further example, an expression repression system may comprise four expression repressors, wherein the first and second expression repressors both comprise a first repressor domain and the third and fourth expression repressors comprises a second different repressor domain. For a further example, an expression repression system may comprise five expression repressors, wherein the first and second expression repressors both comprise a first repressor domain, the third and fourth expression repressors both comprise a second different repressor domain, and the fifth expression repressor comprises a third different repressor domain. As described above, different can mean comprising different types of repressor domain or comprising distinct variants of the same type of repressor domain.
In some embodiments, two repressor domains comprise a histone methyltransferase activity (e.g., SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, or a functional variant or fragment of any thereof, e.g., a SET domain of any thereof) and the other repressor domain comprises a histone demethylase activity (e.g., KDM1A
(i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, or a functional variant or fragment of any thereof). In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a histone deacetylase activity (e.g., HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof). In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a DNA methyltransferase activity (e.g., MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L, or a functional variant or fragment of any thereof). In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a DNA demethylase activity. In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a transcription repressor activity (e.g., KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment of any thereof). In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises a different histone methyltransferase activity. In some embodiments, two repressor domains comprise a histone methyltransferase activity and the other repressor domain comprises the same histone methyltransferase activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a histone deacetylase activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a DNA
methyltransferase activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a DNA demethylase activity.
In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a transcription repressor activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises a different histone demethylase activity. In some embodiments, two repressor domains comprise a histone demethylase activity and the other repressor domain comprises the same histone demethylase activity. In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises a DNA
methyltransferase activity. In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises a DNA demethylase activity.
In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises a transcription repressor activity. In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises a different histone deacetylase activity. In some embodiments, two repressor domains comprise a histone deacetylase activity and the other repressor domain comprises the same histone deacetylase activity. In some embodiments, two repressor domains comprise a DNA methyltransferase activity and the other repressor domain comprises a DNA
demethylase activity. In some embodiments, two repressor domains comprise a DNA methyltransferase activity and the other repressor domain comprises a transcription repressor activity. In some embodiments, two repressor domains comprise a DNA methyltransferase activity and the other repressor domain comprises a different DNA methyltransferase activity. In some embodiments, two repressor domains comprise a DNA methyltransferase activity and the other repressor domain comprises the same DNA methyltransferase activity. In some embodiments, two repressor domains comprise a DNA
demethylase activity and the other repressor domain comprises a transcription repressor activity. In some embodiments, two repressor domains comprise a DNA demethylase activity and the other repressor domain comprises a different DNA demethylase activity. In some embodiments, two repressor domains comprise a DNA demethylase activity and the other repressor domain comprises the same DNA
demethylase activity. In some embodiments, two repressor domains comprise a transcription repressor activity and the other repressor domain comprises a different transcription repressor activity. In some embodiments, two repressor domains comprise a transcription repressor activity and the other repressor domain comprises the same transcription repressor activity.
In some embodiments, two or more (e.g., all) expression repressors of an expression repression system are not covalently associated with each other, e.g., each expression repressor is not covalently associated with any other expression repressor. In another embodiment, two or more expression repressors of an expression repression system are covalently associated with one another. In an 5 embodiment, an expression repression system comprises a first expression repressor and a second expression repressor disposed on the same polypeptide, e.g., as a fusion molecule, e.g., connected by a peptide bond and optionally a linker. In an embodiment, an expression repression system comprises a first expression repressor and a second expression repressor that are connected by a non-peptide bond, e.g., are conjugated to one another.
Linkers An expression repressor or an expression repression system as disclosed herein may comprise one or more linkers. A linker may connect a DNA-targeting moiety to a repressor domain, a repressor domain to another repressor domain, or a DNA-targeting moiety to another DNA-targeting moiety. A linker may be a chemical bond, e.g., one or more covalent bonds or non-covalent bonds. In some embodiments, a linker is covalent. In some embodiments, a linker is non-covalent. In some embodiments, a linker is a peptide linker. Such a linker may be between 2-30, 5-30, 10-30, 15-30, 20-30, 25-30, 2-25, 5-25, 10-25, 15-25, 20-25, 2-20, 5-20, 10-20, 15-20, 2-15, 5-15, 10-15, 2-10, 5-10, or 2-5 amino acids in length, or greater than or equal to 2, 5, 10, 15, 20, 25, or 30 amino acids in length (and optionally up to 50, 40, 30, 25, 20, 15, 10, or 5 amino acids in length). In some embodiments, a linker can be used to space a first domain or moiety from a second domain or moiety, e.g., a DNA-targeting moiety from a repressor domain. In some embodiments, for example, a linker can be positioned between a DNA-targeting moiety and a repressor domain, e.g., to provide molecular flexibility of secondary and tertiary structures. A
linker may comprise flexible, rigid, and/or cleavable linkers described herein. In some embodiments, a linker includes at least one glycine, alanine, and serine amino acids to provide for flexibility. In some embodiments, a linker is a hydrophobic linker, such as including a negatively charged sulfonate group, polyethylene glycol (PEG) group, or pyrophosphate diester group. In some embodiments, a linker is cleavable to selectively release a moiety (e.g., polypeptide) from a modulating agent, but sufficiently stable to prevent premature cleavage.
In some embodiments, one or more moieties and/or domains of an expression repressor described herein are linked with one or more linkers. In some embodiments, an expression repression may comprise a linker situated between the DNA-targeting moiety and the repressor domain. In some embodiments, an expression repressor may comprise a first linker situated between the DNA-targeting moiety and the first repressor domain, and a second linker situated between the DNA-targeting moiety and the second repressor domain. In some embodiments, the first and the second linker may be identical.
In some embodiments, the first and the second linker may be different.
As will be known by one of skill in the art, commonly used flexible linkers have sequences consisting primarily of stretches of Gly and Ser residues ("GS" linker).
Flexible linkers may be useful for joining domains/moieties that require a certain degree of movement or interaction and may include small, non-polar (e.g., Gly) or polar (e.g., Ser or Thr) amino acids. Incorporation of Ser or Thr can also maintain the stability of a linker in aqueous solutions by forming hydrogen bonds with water molecules, and therefore reduce unfavorable interactions between a linker and moieties/domains.
Rigid linkers are useful to keep a fixed distance between domains/moieties and to maintain their independent functions. Rigid linkers may also be useful when a spatial separation of domains is critical to preserve the stability or bioactivity of one or more components in the fusion.
Rigid linkers may have an alpha helix-structure or Pro-rich sequence, (XP)11, with X designating any amino acid, preferably Ala, Lys, or Glu.
Cleavable linkers may release free functional domains in vivo. In some embodiments, linkers may be cleaved under specific conditions, such as presence of reducing reagents or proteases. In vivo cleavable linkers may utilize reversible nature of a disulfide bond. One example includes a thrombin-sensitive sequence (e.g., PRS) between the two Cys residues. In vitro thrombin treatment of CPRSC
results in the cleavage of a thrombin-sensitive sequence, while a reversible disulfide linkage remains intact. Such linkers are known and described, e.g., in Chen et al. 2013.
Fusion Protein Linkers: Property, Design and Functionality. Adv Drug Deliv. Rev. 65(10): 1357-1369. /n vivo cleavage of linkers in fusions may also be carried out by proteases that are expressed in vivo under certain conditions, in specific cells or tissues, or constrained within certain cellular compartments. Specificity of many proteases offers slower cleavage of the linker in constrained compartments.
Examples of molecules suitable for use in linkers described herein include a negatively charged sulfonate group; lipids, such as a poly (--CH2--) hydrocarbon chains, such as polyethylene glycol (PEG) group, unsaturated variants thereof, hydroxylated variants thereof, amidated or otherwise N-containing variants thereof; noncarbon linkers; carbohydrate linkers; phosphodiester linkers, or other molecule capable of covalently linking two or more components of an expression repressor. Non-covalent linkers are also included, such as hydrophobic lipid globules to which the polypeptide is linked, for example through a hydrophobic region of a polypeptide or a hydrophobic extension of a polypeptide, such as a series of residues rich in leucine, isoleucine, valine, or perhaps also alanine, phenylalanine, or even tyrosine, methionine, glycine, or other hydrophobic residue. Components of an expression repressor may be linked using charge-based chemistry, such that a positively charged component of an expression repressor is linked to a negative charge of another component.
Nucleic acids In one aspect, the disclosure provides nucleic acid sequences encoding an expression repressor, an expression repression system, a DNA-targeting moiety and/or a repressor domain as described herein.
A skilled artisan is aware that the nucleic acid sequences of RNA are identical to the corresponding DNA
sequences, except that typically thymine (T) is replaced by uracil (U). It will be understood that when a nucleotide sequence is represented by a DNA sequence (e.g., comprising, A, T, G, C), this disclosure also provides the corresponding RNA sequence (e.g., comprising, A, U, G, C) in which "U" replaces "T."
Conventional notation is used herein to describe polynucleotide sequences: the left-hand end of a single-stranded polynucleotide sequence is the 5'-end; the left-hand direction of a double-stranded polynucleotide sequence is referred to as the 5'-direction.
It will be appreciated by those skilled in the art that due to the degeneracy of the genetic code, a multitude of nucleotide sequences encoding an expression repressor comprising DNA-targeting moiety and/or a repressor domain as described herein may be produced, some of which have similarity, e.g., 90%, 95%, 96%, 97%, 98%, or 99% identity to the nucleic acid sequences disclosed herein. For instance, codons AGA, AGG, CGA, CGC, CGG, and CGU all encode the amino acid arginine.
Thus, at every position in the nucleic acid molecules of the disclosure where an arginine is specified by a codon, the codon can be altered to any of the corresponding codons described above without altering the encoded polypeptide.
In some embodiments a nucleic acid sequence encoding an expression repressor comprising a DNA-targeting moiety and/or one or more repressor domains may be part or all of a codon-optimized coding region, optimized according to codon usage in mammals, e.g., humans. In some embodiments, a nucleic acid sequence encoding a DNA-targeting moiety and/or one or more repressor domains is codon optimized for increasing the protein expression and/or increasing the duration of protein expression. In some embodiments, a protein produced by the codon optimized nucleic acid sequence is at least 1%, at least 2%, at least 5%, at least 10%, at least 15%, at least 20%, at least 30%, at least 40%, or at least 50%
higher compared to levels of the protein when encoded by a nucleic acid sequence that is not codon optimized.
In one aspect, the disclosure is directed to a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, e.g., wherein the repressor domain is or comprises MQ1, e.g., bacterial MQ1, or a functional variant or fragment thereof. In some embodiments, MQ1 is Spiroplasma monobiae MQ1, e.g., MQ1 from strain ATCC 33825 and/or corresponding to Uniprot ID
P15840. In some embodiments, MQ1 repressor domain is encoded by a nucleotide sequence of SEQ ID
NO: 47. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ ID
NO: 47 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
AGCAAGGTGGAGAACAAGACCAAGAAGCTGCGGGTGTTCGAGGCCTTCGCCGGCATCGGCG
CCCAGCGGAAGGCCCTGGAGAAGGTGCGGAAGGACGAGTACGAGATCGTGGGCCTGGCCG
AGTGGTACGTGCCCGCCATCGTGATGTACCAGGCCATCCACAACAACTTCCACACCAAGCTG
GAGTACAAGAGCGTGAGCCGGGAGGAGATGATCGACTACCTGGAGAACAAGACCCTGAGCT
GGAACAGCAAGAACCCCGTGAGCAACGGCTACTGGAAGCGGAAGAAGGACGACGAGCTGA
AGATCATCTACAACGCCATCAAGCTGAGCGAGAAGGAGGGCAACATCTTCGACATCCGGGA
CCTGTACAAGCGGACCCTGAAGAACATCGACCTGCTGACCTACAGCTTCCCCTGCCAGGACC
TGAGCCAGCAGGGCATCCAGAAGGGCATGAAGCGGGGCAGCGGCACCCGGAGCGGCCTGCT
GTGGGAGATCGAGCGGGCCCTGGACAGCACCGAGAAGAACGACCTGCCCAAGTACCTGCTG
ATGGAGAACGTGGGCGCCCTGCTGCACAAGAAGAACGAGGAGGAGCTGAACCAGTGGAAG
CAGAAGCTGGAGAGCCTGGGCTACCAGAACAGCATCGAGGTGCTGAACGCCGCCGACTTCG
GCAGCAGCCAGGCCCGGCGGCGGGTGTTCATGATCAGCACCCTGAACGAGTTCGTGGAGCT
GCCCAAGGGCGACAAGAAGCCCAAGAGCATCAAGAAGGTGCTGAACAAGATCGTGAGCGA
GAAGGACATCCTGAACAACCTGCTGAAGTACAACCTGACCGAGTTCAAGAAAACCAAGAGC
AACATCAACAAGGCCAGCCTGATCGGCTACAGCAAGTTCAACAGCGAGGGCTACGTGTACG
ACCCCGAGTTCACCGGCCCCACCCTGACCGCCAGCGGCGCCAACAGCCGGATCAAGATCAA
GGACGGCAGCAACATCCGGAAGATGAACAGCGACGAGACCTTCCTGTACATCGGCTTCGAC
AGCCAGGACGGCAAGCGGGTGAACGAGATCGAGTTCCTGACCGAGAACCAGAAGATCTTCG
TGTGCGGCAACAGCATCAGCGTGGAGGTGCTGGAGGCCATCATCGACAAGATCGGCGGC
(SEQ ID NO: 47) In some embodiments, MQ1 comprises an amino acid sequence of SEQ ID NO: 90. In some embodiments, MQ1 comprises an amino acid sequence of SEQ ID NO: 57. In some embodiments, an effector domain described herein comprises SEQ ID NO: 90 or 57, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
MSKVENKTKKLRVFEAFAGIGAQRKALEKVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLE
YKSVSREEMIDYLENKTLSWNSKNPVSNGYWKRKKDDELKIIYNAIKLSEKEGNIFDIRDLYKRT
LKNIDLLTYSFPCQDLS QQGIQKGMKRGSGTRSGLLWEIERALD S TEKNDLPKYLLMENVGALL
HKKNEEELNQWKQKLESLGYQNSIEVLNAADFGS S QARRRVFMISTLNEFVELPKGDKKPKSIK
KVLNKIVSEKDILNNLLKYNLTEFKKTKSNINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSR
IKIKDGSNIRKMNSDETFLYIGFDSQDGKRVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGG (SEQ
ID NO: 90) SKVENKTKKLRVFEAFAGIGAQRKALEKVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEY
KSVSREEMIDYLENKTLSWNSKNPVSNGYWKRKKDDELKIIYNAIKLSEKEGNIFDIRDLYKRTL
KNIDLLTYSFPCQDLSQQGIQKGMKRGSGTRSGLLWEIERALDSTEKNDLPKYLLMENVGALLH
KKNEEELNQWKQKLESLGYQNSIEVLNAADFGSSQARRRVFMISTLNEFVELPKGDKKPKSIKK
VLNKIVSEKDILNNLLKYNLTEFKKTKSNINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRI
KIKDGSNIRKMNSDETFLYIGFDSQDGKRVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGG (SEQ
ID NO: 57) In some embodiments, MQ1 for use in a polypeptide described herein is a variant, e.g., comprising one or more mutations, relative to wildtype MQ1 (e.g., SEQ ID NO: 90 or SEQ ID NO:
57). In some embodiments, an MQ1 variant comprises one or more amino acid substitutions, deletions, or insertions relative to wildtype MQ1. In some embodiments, an MQ1 variant comprises a K297P substitution. In some embodiments, an MQ1 variant comprises a N299C substitution. In some embodiments, an MQ1 variant comprises a E301Y substitution. In some embodiments, an MQ1 variant comprises a Q147L
substitution (e.g., and has reduced DNA methyltransferase activity relative to wildtype MQ1). In some embodiments, an MQ1 variant comprises K297P, N299C, and E301Y substitutions (e.g., and has reduced DNA binding affinity relative to wildtype MQ1). In some embodiments, an MQ1 variant comprises Q147L, K297P, N299C, and E301Y substitutions (e.g., and has reduced DNA
methyltransferase activity and DNA binding affinity relative to wildtype MQ1). In some embodiments, the polypeptide comprises one or more linkers described herein, e.g., connecting a moiety/domain to another moiety/domain. In some embodiments, the polypeptide comprises a DNA-targeting moiety that is or comprises a CRISPR/Cas molecule, e.g., comprising a CRISPR/Cas protein, e.g., a dCas9 protein. In some embodiments, the polypeptide is a fusion protein comprising a repressor domain that is or comprises MQ1 and a DNA-targeting moiety that is or comprises a CRISPR/Cas molecule, e.g., comprising a CRISPR/Cas protein, e.g., a dCas9 protein. In some embodiments, the polypeptide comprises an additional moiety described herein. In some embodiments, the polypeptide decreases expression of a target gene (e.g., a target gene described herein). In some embodiments, the polypeptide may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene or transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising MQ1, e.g., bacterial MQ1, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide 5 .. comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises Krueppel-associated box (KRAB) e.g., as according to NP_056209.2 or the protein encoded by NM_015394.5 or a functional variant or fragment thereof. In some embodiments, KRAB is a synthetic KRAB construct. In some embodiments, KRAB comprises an amino acid sequence of SEQ ID NO: 61:
DAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVIL
RLEKGEEPWLVEREIHQETHPDSETAFEIKSSV (SEQ ID NO: 61) In some embodiments, the KRAB repressor domain is encoded by a nucleotide sequence of SEQ
ID NO: 51. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ
ID NO: 51 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
GACGCCAAGAGCCTGACCGCCTGGAGCCGGACCCTGGTGACCTTCAAGGACGTGTTCGTGG
ACTTCACCCGGGAGGAGTGGAAGCTGCTGGACACCGCCCAGCAGATCCTGTACCGGAACGT
GATGCTGGAGAACTACAAGAACCTGGTGAGCCTGGGCTACCAGCTGACCAAGCCCGACGTG
ATCCTGCGGCTGGAGAAGGGCGAGGAGCCCTGGCTGGTGGAGCGGGAGATCCACCAGGAGA
CCCACCCCGACAGCGAGACCGCCTTCGAGATCAAGAGCAGCGTG (SEQ ID NO: 51) In some embodiments, KRAB for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the KRAB sequence of SEQ ID
NO: 61. In some embodiments, an KRAB variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 61.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises KRAB and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the KRAB sequence of SEQ ID NO: 61, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, wherein the repressor domain is or comprises DNMT1, e.g., human DNMT1, or a functional variant or fragment thereof In some embodiments, DNMT1 is human DNMT1, e.g., corresponding to Gene ID 1786, e.g., corresponding to UniPort ID P26358.2. In some embodiments, DNMT1 comprises an amino acid sequence of SEQ ID NO: 58. In some embodiments, a repressor domain described herein comprises a sequence according to SEQ ID NO: 58 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto:
VDLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILL
KLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYY
RPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGE
KLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISY
NGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMA
RKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLY
GRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVG
NAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD (SEQ ID NO: 58) In some embodiments, DNMT1 is encoded by a nucleotide sequence of SEQ ID NO:
48. In some embodiments, a nucleic acid described herein comprises a sequence of SEQ ID
NO: 48 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto:
GTGGATCTGAGGACACTCGACGTGTTTAGCGGATGCGGCGGACTCTCCGAAGGCTTCCACCA
AGCCGGAATTTCCGACACACTCTGGGCCATTGAGATGTGGGACCCCGCCGCTCAAGCCTTCA
GACTGAATAATCCCGGCTCCACCGTGTTCACCGAGGACTGCAACATTCTGCTGAAGCTGGTG
ATGGCTGGCGAAACCACCAACTCTAGAGGCCAGAGGCTGCCCCAGAAGGGAGATGTGGAAA
TGCTCTGTGGAGGCCCTCCTTGCCAAGGCTTCTCCGGCATGAACAGGTTCAACTCTAGAACA
TACAGCAAGTTCAAGAACTCTCTGGTCGTGAGCTTTCTGAGCTACTGCGACTACTATAGACC
TAGGTTCTTTCTGCTGGAGAACGTGAGAAATTTCGTGTCCTTCAAGAGGAGCATGGTGCTGA
AGCTGACACTGAGGTGTCTGGTGAGGATGGGCTACCAGTGCACATTCGGAGTGCTGCAAGCT
GGCCAGTACGGCGTGGCCCAGACCAGAAGGAGGGCCATCATTCTGGCTGCTGCCCCCGGCG
AGAAACTCCCTCTGTTCCCCGAGCCCCTCCACGTGTTCGCCCCTAGAGCTTGCCAGCTGAGC
GTGGTGGTCGACGATAAGAAGTTCGTGAGCAACATCACAAGGCTGTCCAGCGGACCCTTCA
GAACCATTACCGTGAGGGATACCATGTCCGACCTCCCCGAGGTGAGGAATGGCGCCAGCGC
TCTGGAGATTTCCTACAACGGCGAACCTCAGAGCTGGTTCCAAAGGCAGCTGAGAGGCGCTC
AGTATCAGCCCATTCTGAGGGACCACATCTGCAAAGATATGAGCGCTCTGGTGGCCGCTAGA
ATGAGACATATTCCTCTGGCCCCCGGCAGCGACTGGAGAGATCTGCCCAATATTGAGGTGAG
ACTCAGCGACGGAACAATGGCTAGAAAACTGAGGTACACCCATCATGATAGAAAGAACGGA
AGGAGCAGCAGCGGCGCTCTGAGAGGAGTGTGTAGCTGCGTGGAAGCTGGCAAGGCTTGCG
ATCCCGCCGCTAGGCAGTTCAATACCCTCATCCCTTGGTGTCTGCCTCACACCGGCAACAGA
CACAATCATTGGGCTGGACTGTATGGAAGGCTCGAATGGGACGGCTTTTTCAGCACCACCGT
GACCAATCCCGAACCTATGGGCAAGCAAGGAAGGGTGCTCCACCCCGAGCAGCATAGAGTC
GTGTCCGTGAGAGAATGCGCTAGAAGCCAAGGCTTCCCCGACACCTATAGACTGTTCGGCAA
CATTCTGGATAAGCACAGACAAGTGGGAAATGCTGTCCCTCCTCCTCTGGCCAAGGCTATCG
GACTGGAGATCAAGCTGTGTATGCTCGCCAAAGCTAGGGAGAGCGCTTCCGCCAAGATTAA
GGAGGAGGAGGCCGCCAAGGAC (SEQ ID NO: 48) In some embodiments, DNMT1 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to a DNMT sequence of SEQ ID NO:
58. In some embodiments, the effector domain comprises one or more amino acid substitutions, deletions, or insertions relative to wild type DNMT1. In some embodiments, the polypeptide is a fusion protein comprising a repressor domain that is or comprises DNMT1 and a targeting moiety. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising DNMT1, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, wherein the repressor domain is or comprises DNMT3a/3L complex, or a functional variant or fragment thereof. In some embodiments, the DNMT3a/3L complex is a fusion construct. In some embodiments the DNMT3a/3L complex comprises DNMT3A, e.g., human DNMT3A, e.g., as according to NP_072046.2 or the protein encoded by NM_022552.4). In some embodiments the DNMT3a/3L
complex comprises mouse DNMT3A, e.g., as according to NP_031898 or the protein encoded by NM_007872. In some embodiments the DNMT3a/3L complex comprises human DNMT3L (e.g., as according to NP_787063.1 or the protein encoded by NM_175867.3). In some embodiments the DNMT3a/3L
complex comprises mouse DNMT3L (e.g., as according to NP_001075164 or the protein encoded by NM_001081695). In some embodiments, DNMT3a/3L comprises an amino acid sequence of SEQ ID NO:59 or 60. In some embodiments, a repressor domain described herein comprises SEQ ID NO: 59 or SEQ ID NO: 60, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
NHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRH
QGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDA
RPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRP
LASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFG
FPVHYTDV SNMSRLARQRLLGRSWS VPVIRHLFAPLKEYFACVS SGNSNANSRGPSFS SGLVPLS
LRGSHMNPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKD
VEEWGPFDLVYGATPPLGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDL
DVASRFLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALV SEEELSLLAQNKQS SKLAAKW
PTKLVKNCFLPLREYFKYFSTELTSSL (SEQ ID NO: 59) NHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRH
QGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDA
RPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRP
LASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFG
FPVHYTDV SNMSRLARQRLLGRSWS VPVIRHLFAPLKEYFACVS SGNSNANSRGPSFS SGLVPLS
LRGSHMGPMEIYKTVSAWKRQPVRVLSLFRNIDKVLKSLGFLESGSGSGGGTLKYVEDVTNVVR
RDVEKWGPFDLVYGSTQPLGS SCDRCPGWYMFQFHRILQYALPRQES QRPFFWIFMDNLLLTED
DQETTTRFLQTEAVTLQDVRGRDYQNAMRVWSNIPGLKSKHAPLTPKEEEYLQAQVRSRSKLD
APKVDLLVKNCLLPLREYFKYFSQNSLPL (SEQ ID NO: 60) In some embodiments, DNMT3a/3L is encoded by a nucleotide sequence of SEQ ID
NO: 49 or SEQ ID NO: 50. In some embodiments, a nucleic acid described herein comprises a sequence of SEQ ID
NO: 49 or 50 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
AACCACGACCAGGAGTTCGACCCCCCCAAGGTGTACCCCCCCGTGCCCGCCGAGAAGCGGA
AGCCCATCCGGGTGCTGAGCCTGTTCGACGGCATCGCCACCGGCCTGCTGGTGCTGAAGGAC
CTGGGCATCCAGGTGGACCGGTACATCGCCAGCGAGGTGTGCGAGGACAGCATCACCGTGG
GCATGGTGCGGCACCAGGGCAAGATCATGTACGTGGGCGACGTGCGGAGCGTGACCCAGAA
GCACATCCAGGAGTGGGGCCCCTTCGACCTGGTGATCGGCGGCAGCCCCTGCAACGACCTG
AGCATCGTGAACCCCGCCCGGAAGGGCCTGTACGAGGGCACCGGCCGGCTGTTCTTCGAGTT
CTACCGGCTGCTGCACGACGCCCGGCCCAAGGAGGGCGACGACCGGCCCTTCTTCTGGCTGT
TCGAGAACGTGGTGGCCATGGGCGTGAGCGACAAGCGGGACATCAGCCGGTTCCTGGAGAG
CAACCCCGTGATGATCGACGCCAAGGAGGTGAGCGCCGCCCACCGGGCCCGGTACTTCTGG
GGCAACCTGCCCGGCATGAACCGGCCCCTGGCCAGCACCGTGAACGACAAGCTGGAGCTGC
AGGAGTGCCTGGAGCACGGCCGGATCGCCAAGTTCAGCAAGGTGCGGACCATCACCACCCG
GAGCAACAGCATCAAGCAGGGCAAGGACCAGCACTTCCCCGTGTTCATGAACGAGAAGGAG
GACATCCTGTGGTGCACCGAGATGGAGCGGGTGTTCGGCTTCCCCGTGCACTACACCGACGT
GAGCAACATGAGCCGGCTGGCCCGGCAGCGGCTGCTGGGCCGGAGCTGGAGCGTGCCCGTG
ATCCGGCACCTGTTCGCCCCCCTGAAGGAGTACTTCGCCTGCGTGAGCAGCGGCAACAGCAA
CGCCAACAGCCGGGGCCCCAGCTTCAGCAGCGGCCTGGTGCCCCTGAGCCTGCGGGGCAGC
CACATGAATCCTCTGGAGATGTTCGAGACAGTGCCCGTGTGGAGAAGGCAACCCGTGAGGG
TGCTGAGCCTCTTCGAGGACATTAAGAAGGAGCTGACCTCTCTGGGCTTTCTGGAATCCGGC
AGCGACCCCGGCCAGCTGAAACACGTGGTGGACGTGACCGACACAGTGAGGAAGGACGTGG
AAGAGTGGGGCCCCTTTGACCTCGTGTATGGAGCCACACCTCCTCTCGGCCACACATGCGAT
AGGCCTCCCAGCTGGTATCTCTTCCAGTTCCACAGACTGCTCCAGTACGCCAGACCTAAGCC
CGGCAGCCCCAGACCCTTCTTCTGGATGTTCGTGGACAATCTGGTGCTGAACAAGGAGGATC
TGGATGTGGCCAGCAGATTTCTGGAGATGGAACCCGTGACAATCCCCGACGTGCATGGCGG
CTCTCTGCAGAACGCCGTGAGAGTGTGGTCCAACATCCCCGCCATTAGAAGCAGACACTGGG
CTCTGGTGAGCGAGGAGGAACTGTCTCTGCTGGCCCAGAATAAGCAGTCCTCCAAGCTGGCC
GCCAAGTGGCCCACCAAGCTGGTGAAGAACTGCTTTCTGCCTCTGAGGGAGTATTTCAAGTA
TTTCAGCACCGAACTGACCAGCAGCCTG (SEQ ID NO: 49) AACCACGACCAGGAGTTCGACCCCCCCAAGGTGTACCCCCCCGTGCCCGCCGAGAAGCGGA
AGCCCATCCGGGTGCTGAGCCTGTTCGACGGCATCGCCACCGGCCTGCTGGTGCTGAAGGAC
CTGGGCATCCAGGTGGACCGGTACATCGCCAGCGAGGTGTGCGAGGACAGCATCACCGTGG
GCATGGTGCGGCACCAGGGCAAGATCATGTACGTGGGCGACGTGCGGAGCGTGACCCAGAA
GCACATCCAGGAGTGGGGCCCCTTCGACCTGGTGATCGGCGGCAGCCCCTGCAACGACCTG
AGCATCGTGAACCCCGCCCGGAAGGGCCTGTACGAGGGCACCGGCCGGCTGTTCTTCGAGTT
CTACCGGCTGCTGCACGACGCCCGGCCCAAGGAGGGCGACGACCGGCCCTTCTTCTGGCTGT
TCGAGAACGTGGTGGCCATGGGCGTGAGCGACAAGCGGGACATCAGCCGGTTCCTGGAGAG
CAACCCCGTGATGATCGACGCCAAGGAGGTGAGCGCCGCCCACCGGGCCCGGTACTTCTGG
GGCAACCTGCCCGGCATGAACCGGCCCCTGGCCAGCACCGTGAACGACAAGCTGGAGCTGC
AGGAGTGCCTGGAGCACGGCCGGATCGCCAAGTTCAGCAAGGTGCGGACCATCACCACCCG
GAGCAACAGCATCAAGCAGGGCAAGGACCAGCACTTCCCCGTGTTCATGAACGAGAAGGAG
GAGCAACATGAGCCGGCTGGCCCGGCAGCGGCTGCTGGGCCGGAGCTGGAGCGTGCCCGTG
ATCCGGCACCTGTTCGCCCCCCTGAAGGAGTACTTCGCCTGCGTGAGCAGCGGCAACAGCAA
CGCCAACAGCCGGGGCCCCAGCTTCAGCAGCGGCCTGGTGCCCCTGAGCCTGCGGGGCAGC
CACATGGGCCCCATGGAGATCTACAAGACCGTGAGCGCCTGGAAGCGGCAGCCCGTGCGGG
AGCGGCAGCGGCGGCGGCACCCTGAAGTACGTGGAGGACGTGACCAACGTGGTGCGGCGGG
ACGTGGAGAAGTGGGGCCCCTTCGACCTGGTGTACGGCAGCACCCAGCCCCTGGGCAGCAG
CTGCGACCGGTGCCCCGGCTGGTACATGTTCCAGTTCCACCGGATCCTGCAGTACGCCCTGC
CCCGGCAGGAGAGCCAGCGGCCCTTCTTCTGGATCTTCATGGACAACCTGCTGCTGACCGAG
GGGGCCGGGACTACCAGAACGCCATGCGGGTGTGGAGCAACATCCCCGGCCTGAAATCCAA
GCACGCCCCCCTGACCCCCAAGGAGGAGGAGTACCTGCAGGCCCAGGTGCGGAGCCGGAGC
AAGCTGGACGCCCCCAAGGTGGACCTGCTGGTGAAGAACTGCCTGCTGCCCCTGCGGGAGT
ACTTCAAGTACTTCAGCCAGAACAGCCTGCCCCTG (SEQ ID NO: 50) In some embodiments, DNMT3a/3L for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the DNMT3a/3L of SEQ ID NO:
59 or SEQ ID NO: 60. In some embodiments, an DNMT3a/3L variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 59 or SEQ ID
NO: 60. In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises DNMT3a/3L and a DNA-targeting moiety. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising DNMT3a/3L, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, wherein the repressor domain is or comprises DNMT3b, e.g., human DNMT3b, or a functional variant or fragment thereof. In some embodiments the DNMT3b is human DNMT3b e.g., as according to NP_008823.1 or A0X21819.1, or the protein encoded by NM_006892.4 or KX447429.) In some embodiments, DNMT3b comprises an amino acid sequence of SEQ ID NO: 85. In some embodiments, a repressor domain described herein comprises SEQ ID NO: 85, or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
DNMT3b MKGDTRHLNGEEDAGGRED SILVNGAC SD QS SD SPPILEAIRTPEIRGRRS S SRLS KREVS SLLS YT
QDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRN
HVDESPVEFPATRS LRRRATAS AGTPWPSPPS S YLTIDLTDDTEDTHGTPQS S S TPYARLAQD S QQ
GGMESPQVEADSGDGDSSEYQVSADKLVALGLFSQHFNLATFNKLVSYRKAMYHALEKARVR
AGKTFPSSPGDSLEDQLKPMLEWAHGGFKPTGIEGLKPNNTQPENKTRRRTADDSATSDYCPAP
KRLKTNCYNNGKDRGDEDQSREQMASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCR
DRFLELFYMYDDDGYQSYCTVCCEGRELLLCSNTSCCRCFCVECLEVLVGTGTAAEAKLQEPWS
CYMCLPQRCHGVLRRRKDWNVRLQAFFTSDTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGY
LVLKELGIKVGKYVASEVCEESIAVGTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCND
LSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRFLECN
PVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEYNRIAKLKKVQTITTKSNSIK
QGKNQLFPVVMNGKEDVLWCTELERIFGFPVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAP
LKDYFACE (SEQ ID NO: 85) In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises G9A e.g., as according to NP_001350618.1 or the protein encoded by NM_001363689.1 or a functional variant or fragment thereof. In some embodiments, G9A comprises an amino acid sequence of SEQ ID NO: 62:
GNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCV
DDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQL
YRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYG
NISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGS
EKCKHSAEAIALEQSRLARLD (SEQ ID NO: 62) In some embodiments, the G9A repressor domain is encoded by a nucleotide sequence of SEQ ID
NO: 52. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ ID
NO: 52 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
GGAAATAGGGCTATCAGAACCGAGAAGATCATCTGTAGGGACGTGGCTAGAGGCTACGAGA
ACGTGCCCATTCCTTGCGTGAATGGCGTGGATGGCGAACCTTGCCCCGAGGACTACAAATAC
ATCTCCGAGAACTGCGAAACCAGCACAATGAACATCGACAGAAACATCACCCACCTCCAGC
ACTGCACATGTGTGGATGACTGCTCCTCCAGCAACTGTCTGTGCGGCCAGCTCTCCATCAGA
TGCTGGTACGACAAGGACGGCAGACTGCTGCAAGAGTTCAACAAGATCGAACCCCCTCTCA
TCTTCGAGTGTAACCAAGCTTGCAGCTGCTGGAGAAACTGCAAGAATAGAGTGGTCCAGAG
CGGCATCAAGGTGAGACTGCAACTGTACAGAACCGCCAAGATGGGATGGGGAGTGAGGGCT
CTGCAAACCATTCCCCAAGGCACCTTCATCTGCGAATACGTGGGCGAACTGATCTCCGACGC
CGAAGCTGACGTGAGAGAGGACGACAGCTATCTCTTCGATCTGGACAATAAGGACGGCGAG
GTGTACTGCATCGACGCTAGATATTACGGCAACATCTCTAGATTCATCAACCACCTCTGCGA
TCCCAACATCATTCCCGTGAGGGTGTTCATGCTGCACCAAGATCTGAGGTTCCCTAGAATCG
CCTTCTTCAGCTCTAGAGACATCAGAACCGGCGAGGAGCTGGGCTTCGATTACGGCGATAGA
TTCTGGGACATCAAGTCCAAGTACTTCACATGCCAGTGCGGCAGCGAGAAGTGTAAGCACA
GCGCTGAGGCCATTGCTCTGGAGCAGTCTAGACTGGCCAGACTGGAT (SEQ ID NO: 52) In some embodiments, G9A for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the G9A
sequence of SEQ ID NO: 62. In some embodiments, an G9A variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 62.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises G9A and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the G9A sequence of SEQ ID NO: 62, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises HDAC8, e.g., as according to NP_001159890 or the protein encoded by NM_001166418 or a functional variant or fragment thereof. In some embodiments, HDAC8 comprises an amino acid sequence of SEQ ID NO: 63:
EEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEE
MATFHTDAYLQHLQKVS QEGDDDHPD SIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLID GM
CKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTS
KVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNP
KAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTG
VILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV (SEQ ID NO:
63) In some embodiments, the HDAC8 repressor domain is encoded by a nucleotide sequence of SEQ ID NO: 53. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ ID NO: 53 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
GAGGAGCCCGAGGAGCCCGCCGATAGCGGACAATCTCTGGTGCCCGTCTACATCTACAGCC
CCGAATATGTGAGCATGTGTGATTCCCTCGCCAAGATCCCTAAGAGAGCCAGCATGGTGCAT
TCTCTGATCGAGGCCTACGCTCTGCATAAGCAAATGAGGATCGTGAAGCCCAAGGTCGCCAG
CATGGAAGAGATGGCCACCTTTCACACCGATGCCTACCTCCAACATCTCCAGAAGGTGTCCC
AAGAGGGCGACGACGACCACCCCGACTCCATTGAGTACGGACTGGGCTATGATTGCCCCGC
CACCGAGGGCATCTTTGACTATGCCGCCGCTATCGGCGGAGCTACCATCACAGCCGCCCAGT
GTCTGATTGATGGCATGTGCAAGGTCGCCATCAACTGGTCCGGAGGCTGGCATCATGCCAAG
AAGGATGAGGCCTCCGGCTTCTGTTATCTGAATGACGCCGTGCTGGGCATTCTGAGACTGAG
GAGGAAATTCGAGAGGATTCTGTACGTGGATCTGGATCTGCATCACGGAGATGGAGTCGAA
GATGCCTTCAGCTTCACCAGCAAGGTGATGACAGTCTCTCTGCACAAGTTCTCCCCCGGCTTC
TTTCCCGGAACCGGCGACGTGTCCGACGTGGGACTGGGCAAGGGAAGGTACTACAGCGTGA
ACGTGCCCATTCAAGACGGCATCCAAGACGAGAAGTACTACCAGATCTGCGAGTCCGTGCTC
AAGGAGGTCTACCAAGCCTTCAATCCTAAGGCTGTCGTGCTCCAACTGGGAGCTGATACCAT
TGCTGGCGATCCCATGTGCAGCTTCAATATGACACCCGTCGGAATCGGCAAGTGCCTCAAGT
ACATCCTCCAGTGGCAGCTCGCCACCCTCATTCTCGGAGGAGGCGGATACAATCTGGCTAAT
ACCGCCAGATGCTGGACCTATCTGACCGGCGTGATTCTGGGCAAAACACTGAGCAGCGAAA
TCCCCGACCACGAGTTTTTCACCGCTTACGGCCCCGACTACGTGCTGGAGATCACCCCCAGC
TGCAGACCCGATAGAAACGAACCCCATAGAATCCAGCAAATTCTGAACTATATCAAGGGCA
ACCTCAAGCACGTCGTG (SEQ ID NO: 53) In some embodiments, HDAC8 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the HDAC8 sequence of SEQ ID
NO: 63. In some embodiments, an HDAC8 variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 63.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises HDAC8 and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the HDAC8 sequence of SEQ ID NO: 63, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises LSD1 e.g., as according to NP_055828.2 or the protein encoded by NM_015013.4 or a functional variant or fragment thereof. In some embodiments, KRAB
comprises an amino acid sequence of SEQ ID NO: 64:
LSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKK
EPPRASPPGGLAEPPGSAGPQAGPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLA
NLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEA
ACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERH
GLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGN
YVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRL
LEATSYLSHQLDFNVLNNKHVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKM
VNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEA
NPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVA
LAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPP
LVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSS
GNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYT
LPRQATPGVPAQQSPSM (SEQ ID NO: 64) 10 In some embodiments, the LSD1 repressor domain is encoded by a nucleotide sequence of SEQ
ID NO: 54. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ
ID NO: 54 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
AGCTGGCCCCGGAACAGCTGGCGGAAGCGAAAACGGAAGCGAAGTGGCTGCCCAGCCCGCC
GGACTGTCCGGACCCGCCGAGGTGGGACCCGGCGCTGTCGGCGAAAGGACCCCCAGAAAAA
AGGAGCCTCCTAGAGCCAGCCCTCCCGGCGGACTCGCCGAACCTCCCGGCAGCGCTGGACCT
CAAGCCGGACCTACAGTGGTGCCCGGCAGCGCTACACCTATGGAGACCGGCATCGCTGAGA
ATGAGTCTCTGGCTAACCTCAGCGAGGACGAGTACTACTCCGAGGAGGAAAGGAATGCCAA
GGCCGAGAAGGAGAAGAAGCTGCCCCCTCCTCCCCCTCAAGCCCCCCCCGAGGAGGAGAAC
GAGTCCGAGCCCGAGGAACCCAGCGGAGTGGAAGGAGCCGCCTTTCAGTCCAGACTGCCCC
ACGACAGAATGACATCCCAAGAGGCCGCTTGCTTTCCCGACATTATTTCCGGCCCTCAGCAG
GATCCAGCTGACCTTCGAGGCTACCCTCCAACAGCTGGAGGCCCCCTACAATAGCGATACCG
TGCTGGTGCACAGAGTGCACAGCTATCTGGAGAGGCACGGCCTCATTAACTTCGGCATTTAC
AAGCGGATCAAGCCCCTGCCCACCAAGAAAACAGGCAAGGTGATCATCATCGGCAGCGGCG
TGAGCGGCCTGGCCGCCGCCCGGCAGCTGCAGAGCTTCGGCATGGACGTGACCCTGCTGGA
CTGGGCGCCATGGTGGTGACCGGCCTGGGCGGCAACCCCATGGCCGTGGTGAGCAAGCAGG
TGAACATGGAGCTGGCCAAGATCAAGCAGAAGTGCCCCCTGTACGAGGCCAACGGCCAGGC
CGTGCCCAAGGAGAAGGACGAGATGGTGGAGCAGGAGTTCAACCGGCTGCTGGAGGCCACC
AGCTACCTGAGCCACCAGCTGGACTTCAACGTGCTGAACAACAAGCACGTGAGCCTGGGCC
AGGCCCTGGAGGTGGTGATCCAGCTGCAGGAGAAGCACGTGAAGGACGAGCAGATCGAGC
ACTGGAAGAAGATCGTGAAAACACAGGAGGAGCTGAAGGAGCTGCTGAACAAGATGGTGA
ACCTGAAGGAGAAGATCAAGGAGCTGCACCAGCAGTACAAGGAGGCCAGCGAGGTGAAGC
CCCCCCGGGACATCACCGCCGAGTTCCTGGTCAAAAGCAAGCACCGGGACCTGACCGCCCT
GTGCAAGGAGTACGACGAGCTGGCCGAGACCCAGGGCAAGCTGGAGGAGAAGCTGCAGGA
GCTGGAGGCCAACCCCCCCAGCGACGTGTACCTGAGCAGCCGGGACCGGCAGATCCTGGAC
TGGCACTTCGCCAACCTGGAGTTCGCCAACGCCACCCCCCTGAGCACCCTGAGCCTGAAGCA
CTGGGACCAGGACGACGACTTCGAGTTCACCGGCAGCCACCTGACCGTGCGGAACGGCTAC
AGCTGCGTGCCCGTGGCCCTGGCCGAGGGCCTGGACATCAAGCTGAACACCGCCGTGCGGC
AGGTGCGGTACACCGCCAGCGGCTGCGAGGTGATCGCCGTGAACACCCGGAGCACCAGCCA
GACCTTCATCTACAAGTGCGACGCCGTGCTGTGCACCCTGCCCCTGGGCGTGCTGAAGCAGC
AGCCCCCCGCCGTGCAGTTCGTGCCCCCCCTGCCCGAGTGGAAAACAAGCGCCGTGCAGCG
GATGGGCTTCGGCAACCTGAACAAGGTGGTGCTGTGCTTCGACCGGGTGTTCTGGGACCCCA
GCGTGAACCTGTTCGGCCACGTGGGCAGCACCACCGCCAGCCGGGGCGAGCTGTTCCTGTTC
TGGAACCTGTACAAGGCCCCCATCCTGCTGGCCCTGGTGGCCGGCGAGGCCGCCGGCATCAT
GGAGAACATCAGCGACGACGTGATCGTGGGCCGGTGCCTGGCCATCCTGAAGGGCATCTTC
GGCAGCAGCGCCGTGCCCCAGCCCAAGGAGACCGTGGTGAGCCGGTGGCGGGCCGACCCCT
GGGCCCGGGGCAGCTACAGCTACGTGGCCGCCGGCAGCAGCGGCAACGACTACGACCTGAT
GGCCCAGCCCATCACCCCCGGCCCCAGCATCCCCGGCGCCCCCCAGCCCATCCCCCGGCTGT
TCTTCGCCGGCGAGCACACCATCCGGAACTACCCCGCCACCGTGCACGGCGCCCTGCTGAGC
GGCCTGCGGGAGGCAGGACGGATCGCCGACCAGTTCCTGGGCGCCATGTACACCCTGCCCC
GGCAGGCCACCCCCGGCGTGCCCGCCCAGCAGAGCCCCAGCATG (SEQ ID NO: 54) In some embodiments, LSD1 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the LSD1 sequence of SEQ ID NO: 64. In some embodiments, an LSD1 variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 64.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises LSD1 and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the LSD1 sequence of SEQ ID NO: 64, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises EZH2, e.g., as according to NP-004447.2 or the protein encoded by NM_004456.5 or a functional variant or fragment thereof. In some embodiments, EZH2 comprises an amino acid sequence of SEQ ID NO: 65:
GQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFS SNRQKILERTEILNQEWKQRRI
QPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNI
PYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEE
REEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECT
PNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ
HLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNS SRPS TPTINVLESKDTD SDREAGTETGGEN
NDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLI
GTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQP
CDHPRQPCD S SCPCVIA QNFCEKFCQC S SECQNRFPGCRC KAQCNTKQCPCYLAVRECDPDLCLT
CGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEAD
RRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKR
AIQTGEELFFDYRYSQADALKYVGIEREMEIP (SEQ ID NO: 65) In some embodiments, the EZH2 repressor domain is encoded by a nucleotide sequence of SEQ
ID NO: 55. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ
ID NO: 55 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
GGCCAGACCGGCAAGAAGAGCGAGAAGGGCCCCGTGTGCTGGCGGAAGCGGGTGAAGAGC
GAGTACATGCGGCTGCGGCAGCTGAAGCGGTTCCGGCGGGCCGACGAGGTGAAGAGCATGT
TCAGCAGCAACCGGCAGAAGATCCTGGAGCGGACCGAGATCCTGAACCAGGAGTGGAAGCA
GCGGCGAATCCAGCCCGTGCACATCCTGACCAGCGTGAGCAGCCTGCGGGGCACCCGGGAG
TGCAGCGTGACCAGCGACCTGGACTTCCCCACCCAGGTGATCCCCCTAAAGACCCTGAACGC
CGTGGCCAGCGTGCCCATCATGTACAGCTGGAGCCCCCTGCAGCAGAACTTCATGGTGGAGG
ACGAGACCGTGCTGCACAACATCCCCTACATGGGCGACGAGGTGCTGGACCAGGACGGCAC
CTTCATCGAGGAGCTGATCAAGAACTACGACGGCAAGGTGCACGGCGACCGGGAGTGCGGC
TTCATCAACGACGAGATCTTCGTGGAGCTGGTGAACGCCCTGGGCCAGTACAACGACGACG
ACGACGACGACGACGGCGACGACCCCGAGGAGCGGGAGGAGAAGCAGAAGGACCTGGAGG
ACCACCGGGACGACAAGGAGAGCCGGCCCCCCCGGAAGTTCCCCAGCGACAAGATCTTCGA
GGCCATCAGCAGCATGTTCCCCGACAAGGGCACCGCCGAGGAGCTGAAGGAGAAGTACAAG
GAGCTGACCGAGCAGCAGCTGCCCGGCGCCCTGCCCCCCGAGTGCACCCCCAACATCGACG
GCCCCAACGCCAAGAGCGTGCAGCGGGAGCAGAGCCTGCACAGCTTCCACACCCTGTTCTG
CCGGCGGTGCTTCAAGTACGACTGCTTCCTGCACCCCTTCCACGCCACCCCCAACACCTACA
AGCGGAAGAACACCGAGACCGCCCTGGACAACAAGCCCTGCGGCCCCCAGTGCTACCAGCA
CCTGGAGGGCGCCAAGGAGTTCGCCGCCGCCCTGACCGCCGAGCGGATCAAGACCCCCCCC
AAGCGGCCCGGCGGCCGGCGGCGGGGCCGGCTGCCCAACAACAGCAGCCGGCCCAGCACCC
CCACCATCAACGTGCTGGAGAGCAAGGACACCGACAGCGACCGGGAGGCCGGCACCGAGA
CCGGCGGCGAGAACAACGACAAGGAGGAGGAGGAGAAGAAGGACGAGACCAGCAGCAGC
AGCGAGGCCAACAGCCGGTGCCAGACCCCCATCAAGATGAAGCCCAACATCGAGCCCCCCG
AGAACGTGGAGTGGAGCGGCGCCGAGGCCAGCATGTTCCGGGTGCTGATCGGCACCTACTA
CGACAACTTCTGCGCCATCGCCCGGCTGATCGGCACCAAGACCTGCCGGCAGGTGTACGAGT
TCCGGGTGAAGGAGAGCAGCATCATCGCCCCCGCCCCCGCCGAGGACGTGGACACCCCCCC
CCGGAAGAAGAAGCGGAAGCACCGGCTGTGGGCCGCCCACTGCCGGAAGATCCAGCTGAAG
AAGGACGGCAGCAGCAACCACGTGTACAACTACCAGCCCTGCGACCACCCCCGGCAGCCCT
GCGACAGCAGCTGCCCCTGCGTGATCGCCCAGAACTTCTGCGAGAAGTTCTGCCAGTGCAGC
AGCGAGTGCCAGAACCGGTTCCCCGGCTGCCGGTGCAAGGCCCAGTGCAACACCAAGCAGT
GCCCCTGCTACCTGGCCGTGCGGGAGTGCGACCCCGACCTGTGCCTGACCTGCGGCGCCGCC
GACCACTGGGACAGCAAGAACGTGAGCTGCAAGAACTGCAGCATCCAGCGGGGCAGCAAG
AAGCACCTGCTGCTGGCCCCCAGCGACGTGGCCGGCTGGGGCATCTTCATCAAGGACCCCGT
GCAGAAGAACGAGTTCATCAGCGAGTACTGCGGCGAGATCATCAGCCAGGACGAGGCCGAC
CGGCGGGGCAAGGTGTACGACAAGTACATGTGCAGCTTCCTGTTCAACCTGAACAACGACTT
CGTGGTGGACGCCACCCGGAAGGGCAACAAGATCCGGTTCGCCAACCACAGCGTGAACCCC
AACTGCTACGCCAAGGTGATGATGGTGAACGGCGACCACCGGATCGGCATCTTCGCCAAGC
GGGCCATCCAGACCGGCGAGGAGCTGTTCTTCGACTACCGGTACAGCCAGGCCGACGCCCT
GAAGTACGTGGGCATCGAGCGGGAGATGGAGATCCCC (SEQ ID NO: 55) In some embodiments, EZH2 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the EZH2 sequence of SEQ ID NO: 65. In some embodiments, an EZH2 variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 65.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises EZH2 and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor domain comprising the EZH2 sequence of SEQ ID NO: 65, or a functional variant or fragment thereof.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) targeting moiety and one or more effector moiety, wherein the effector moiety is or comprises FOG1 e.g., as according to NP_722520.2 or the protein encoded by NM_153813.3 or a functional variant or fragment thereof. In some embodiments, FOG1 comprises an amino acid sequence of SEQ ID NO: 66:
SRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPEAPSP (SEQ ID NO: 66) In some embodiments, the FOG1 repressor domain is encoded by a nucleotide sequence of SEQ
ID NO: 56. In some embodiments, a nucleotide sequence described herein comprises a sequence of SEQ
ID NO: 56 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5,4, 3, 2, or 1 positions of difference thereto.
AGCAGAAGGAAGCAGAGCAACCCCAGACAAATCAAGAGATCTCTGGGCGACATGGAGGCC
AGAGAGGAAGTGCAGCTGGTGGGCGCCAGCCACATGGAGCAGAAGGCTACAGCCCCCGAG
GCCCCCAGCCCC (SEQ ID NO: 56) In some embodiments, FOG1 for use in a polypeptide or an expression repressor described herein is a variant, e.g., comprising one or more mutations, relative to the FOG 1 sequence of SEQ ID NO: 66.
In some embodiments, an FOG1 variant comprises one or more amino acid substitutions, deletions, or insertions relative to SEQ ID NO: 66.
In some embodiments, the polypeptide or the expression repressor is a fusion protein comprising a repressor domain that is or comprises FOG1 and a DNA-targeting moiety. In some embodiments, the polypeptide or the expression repressor comprises an additional moiety described herein. In some embodiments, the polypeptide or the expression repressor decreases expression of a target gene. In some 10 embodiments, the polypeptide or the expression repressor may be used in methods of modulating, e.g., decreasing, gene expression, methods of treating a condition, or methods of epigenetically modifying a target gene, e.g., a transcription control element described herein, e.g., in place of an expression repression system. In some embodiments, an expression repression system comprises two or more (e.g., two, three, or four) expression repressors, wherein the first expression repressor comprises a repressor 15 domain comprising the FOG1 sequence of SEQ ID NO: 66, or a functional variant or fragment thereof.
In some embodiments, provided technologies are described as comprising a gRNA
that specifically targets a target gene. In some embodiments, the target gene is an oncogene, a tumor suppressor, or a MYC mis-regulation disorder related gene. In some embodiments, the target gene is 20 MYC. In some embodiments, the target gene is an MHC class I molecule, e.g., I32M. In some embodiments, the target gene encodes a heat shock protein, e.g., HSPA1B. In some embodiments, the target gene is a transcription factor, e.g., GATAl.
In some embodiments, technologies provided herein include methods of delivering one or more 25 expression repressors or expression repression systems described herein to a subject, e.g., to a nucleus of a cell or tissue of a subject, by linking such a moiety to a DNA-targeting moiety as part of a fusion molecule.
In some embodiments, an expression repressor comprises a nuclear localization sequence (NLS).
30 In some embodiments, the expression repressor comprises an NLS, e.g., an 5V40 NLS at the N-terminus.
In some embodiments, the expression repressor comprises an NLS, e.g., a nucleoplasmin NLS at the C-terminus. In some embodiments, the expression repressor comprises a first NLS
at the N-terminus and a second NLS at the C-terminus. In some embodiments the first and the second NLS
have the same sequence. In some embodiments, the first and the second NLS have different sequences. In some embodiments, the expression repression repressor comprises an SV40 NLS, e.g., the expression repressor comprises a sequence according to PKKKRK (SEQ ID NO: 86). In some embodiments, the expression repressor comprises an epitope tag, e.g., an HA tag: YPYDVPDYA (SEQ ID NO:
80). For example, the expression repressor may comprise two copies of the epitope tag.
While an epitope tag is useful in many research contexts, it is sometimes desirable to omit an epitope tag in a therapeutic context. Accordingly, in some embodiments, the expression repressor lacks an epitope tag. In some embodiments, an expression repressor described herein comprises a sequence provided herein (or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto), but lacking the HA tag of SEQ ID NO: 80. In some embodiments, a nucleic acid described herein comprises a sequence provided herein (or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto), but lacking a region encoding the HA tag of SEQ ID NO: 80. In some embodiments, the expression repressor comprises a nucleoplasmin NLS, e.g., the expression repressor comprises a sequence of KRPAATKKAGQAKKK (SEQ ID NO: 87). In some embodiments, the expression repressor does not comprise an NLS. In some embodiments, the expression repressor does not comprise an epitope tag. In some embodiments the expression repressor does not comprise an HA tag. In some embodiments, the expression repressor does not comprise an HA tag sequence according to SEQ ID
NO: 80.
DNA-Targeting Moieties DNA-targeting moieties may specifically bind a DNA sequence, e.g., a DNA
sequence associated with a target gene, e.g., binds, a genomic sequence element (e.g., a promoter, a TSS, or an anchor sequence) in, proximal to, and/or operably linked to a target gene. Any molecule or compound that specifically binds a DNA sequence may be used as a DNA-targeting moiety.
In some embodiments, a DNA-targeting moiety targets, e.g., binds, a component of a genomic complex (e.g., ASMC). In some embodiments, a DNA-targeting moiety targets, e.g., binds, an expression control sequence (e.g., a promoter or enhancer) operably linked to a target gene. In some embodiments, a DNA-targeting moiety targets, e.g., binds, a target gene, or a part of a target gene. The target of a DNA-targeting moiety may be referred to as its targeted component. A targeted component may be any genomic sequence element operably linked to a target gene, or the target gene itself, including but not limited to a promoter, enhancer, anchor sequence, exon, intron, UTR encoding sequence, a splice site, or a transcription start site. In some embodiments, a DNA-targeting moiety binds specifically to one or more target anchor sequences (e.g., within a cell) and not to non-targeted anchor sequences (e.g., within the same cell).
In some embodiments, a DNA-targeting moiety may be or comprise a CRISPR/Cas molecule, a TAL effector molecule, a Zn finger domain, peptide nucleic acid (PNA) or a nucleic acid molecule. In some embodiments, an expression repressor comprises one DNA-targeting moiety.
In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each member of the plurality of expression repressors comprises a DNA-targeting moiety, wherein each DNA-targeting moiety does not detectably bind, e.g., does not bind, to another DNA-targeting moiety. In some embodiments, an expression repression system comprises a first expression repressor comprising a first DNA-targeting moiety and a second expression repressor comprising a second DNA-targeting moiety, wherein the first DNA-targeting moiety does not detectably bind, e.g., does not bind, to the second DNA-targeting moiety. In some embodiments, an expression repression system comprises a first expression repressor comprising a first DNA-targeting moiety and a second expression repressor comprising a second DNA-targeting moiety, wherein the first DNA-targeting moiety does not detectably bind, e.g., does not bind, to another first DNA-targeting moiety, and the second DNA-targeting moiety does not detectably bind, e.g., does not bind, to another second DNA-targeting moiety. In some embodiments, a DNA-targeting moiety for use in the compositions and methods described herein is functional (e.g., binds to a DNA sequence) in a monomeric, e.g., non-dimeric, state.
In some embodiments, binding of a targeting moiety to a targeted component decreases binding affinity of the targeted component for another transcription factor, genomic complex component, or genomic sequence element. In some embodiments, a DNA-targeting moiety binds to its target sequence with a KD of less than or equal to 500, 450, 400, 350, 300, 250, 200, 150, 100, 50, 40, 30, 20, 10, 9, 8, 7, 6, 5, 4, 3, 2, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0.09, 0.08, 0.07, 0.06, 0.05, 0.04, 0.03, 0.02, 0.01, 0.005, 0.002, or 0.001 nM (and optionally, a KD of at least 50, 40, 30, 20, 10, 9, 8, 7, 6, 5, 4, 3, 2, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0.09, 0.08, 0.07, 0.06, 0.05, 0.04, 0.03, 0.02, 0.01, 0.005, 0.002, or 0.001 nM). In some embodiments, a DNA-targeting moiety binds to its target sequence with a KD of 0.001 nM to 500 nM, e.g., 0.1 nM to 5 nM, e.g., about 0.5 nM. In some embodiments, a DNA-targeting moiety binds to a non-target sequence with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or 100,000 nM (and optionally, does not appreciably bind to a non-target sequence). In some embodiments, a DNA-targeting moiety does not bind to a non-target sequence.
In some embodiments, a DNA-targeting moiety comprises a nucleic acid sequence complementary to a targeted component, e.g., a promoter of a target gene. In some embodiments, a targeting moiety comprises a nucleic acid sequence that is at least 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 100%
complementary to a targeted component.
In some embodiments, the DNA-targeting moiety of an expression repressor comprises no more than 100, 90, 80, 70, 60, 50, 40, 30, or 20 nucleotides (and optionally at least 10, 20, 30, 40, 50, 60, 70, 80, or 90 nucleotides). In some embodiments, an expression repressor or a repressor domain of a fusion molecule, comprises no more than 2000, 1900, 1800, 1700, 1600, 1500, 1400, 1300, 1200, 1100, 1000, 900, 800, 700, 600, 500, 400, 300, 200, or 100 amino acids (and optionally at least 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, or 1900 amino acids). In some embodiments, an expression repressor or the effector moiety of a fusion molecule, comprises 100-2000, 100-1900, 100-1800, 100-1700, 100-1600, 100-1500, 100-1400, 100-1300, 100-1200, 100-1100, 100-1000, 100-900, 100-800, 100-700, 100-600, 100-500, 100-400, 100-300, 100-200, 200-2000, 200-1900, 200-1800, 200-1700, 200-1600, 200-1500, 200-1400, 200-1300, 200-1200, 200-1100, 200-1000, 200-900, 200-800, 200-700, 200-600, 200-500, 200-400, 200-300, 300-2000, 300-1900, 300-1800, 300-1700, 300-1600, 300-1500, 300-1400, 300-1300, 300-1200, 300-1100, 300-1000, 300-900, 300-800, 300-700, 300-600, 300-500, 300-400, 400-2000, 400-1900, 400-1800, 400-1700, 400-1600, 400-1500, 400-1400, 400-1300, 400-1200, 400-1100, 400-1000, 400-900, 400-800, 400-700, 400-600, 400-500, 500-2000, 500-1900, 500-1800, 500-1700, 500-1600, 500-1500, 500-1400, 500-1300, 500-1200, 500-1100, 500-1000, 500-900, 500-800, 500-700, 500-600, 600-2000, 600-1900, 600-1800, 600-1700, 600-1600, 600-1500, 600-1400, 600-1300, 600-1200, 600-1100, 600-1000, 600-900, 600-800, 600-700, 700-2000, 700-1900, 700-1800, 700-1700, 700-1600, 700-1500, 700-1400, 700-1300, 700-1200, 700-1100, 700-1000, 700-900, 700-800, 800-2000, 800-1900, 800-1800, 800-1700, 800-1600, 800-1500, 800-1400, 800-1300, 800-1200, 800-1100, 800-1000, 800-900, 900-2000, 900-1900, 900-1800, 900-1700, 900-1600, 900-1500, 900-1400, 900-1300, 900-1200, 900-1100, 900-1000, 1000-2000, 1000-1900, 1000-1800, 1000-1700, 1000-1600, 1000-1500, 1000-1400, 1000-1300, 1000-1200, 1000-1100, 1100-2000, 1100-1900, 1100-1800, 1100-1700, 1100-1600, 1100-1500, 1100-1400, 1100-1300, 1100-1200, 1200-2000, 1200-1900, 1200-1800, 1200-1700, 1200-1600, 1200-1500, 1200-1400, 1200-1300, 1300-2000, 1300-1900, 1300-1800, 1300-1700, 1300-1600, 1300-1500, 1300-1400, 1400-2000, 1400-1900, 1400-1800, 1400-1700, 1400-1600, 1400-1500, 1500-2000, 1500-1900, 1500-1800, 1500-1700, 1500-1600, 1600-2000, 1600-1900, 1600-1800, 1600-1700, 1700-2000, 1700-1900, 1700-1800, 1800-2000, 1800-1900, or 1900-2000 amino acids.
An expression repressor or an expression repression system as disclosed herein, may comprise nucleic acid, e.g., one or more nucleic acids. In some embodiments, a nucleic acid is or comprises RNA;
in some embodiments, a nucleic acid is or comprises DNA. In some embodiments, a nucleic acid is or comprises more than 50% ribonucleotides. In some embodiments, a nucleic acid is, comprises, or consists of one or more natural nucleic acid residues. In some embodiments, a nucleic acid is, comprises, or consists of one or more nucleic acid analogs. In some embodiments, a nucleic acid analog differs from a nucleic acid in that it does not utilize a phosphodiester backbone. For example, in some embodiments, a nucleic acid is, comprises, or consists of one or more peptide nucleic acids.
Alternatively, or additionally, in some embodiments, a nucleic acid has one or more phosphorothioate and/or 5'-N-phosphoramidite linkages rather than phosphodiester bonds. In some embodiments, a nucleic acid is, comprises, or consists of one or more natural nucleosides (e.g., adenosine, thymidine, guanosine, cytidine, uridine, deoxyadenosine, deoxythymidine, deoxy guanosine, and deoxycytidine). In some embodiments, a nucleic .. acid is, comprises, or consists of one or more nucleoside analogs (e.g., 2-aminoadenosine, 2-thiothymidine, inosine, pyrrolo-pyrimidine, 3 -methyl adenosine, 5-methylcytidine, C-5 propynyl-cytidine, C-5 propynyl-uridine, 2-aminoadenosine, C5-bromouridine, C5-fluorouridine, C5-iodouridine, C5-propynyl-uridine, C5 -propynyl-cytidine, C5-methylcytidine, 2-aminoadenosine, 7-deazaadenosine, 7-deazaguanosine, 8-oxoadenosine, 8-oxoguanosine, 0(6)-methylguanine, 2-thiocytidine, methylated bases, intercalated bases, and combinations thereof). In some embodiments, a nucleic acid comprises one or more modified sugars (e.g., 2'-fluororibose, ribose, 2'-deoxyribose, arabinose, and hexose) as compared with those in natural nucleic acids. In some embodiments, a nucleic acid has a nucleotide sequence that encodes a functional gene product such as an RNA or protein. In some embodiments, a nucleic acid includes one or more introns. In some embodiments, nucleic acids are prepared by one or more of .. isolation from a natural source, enzymatic synthesis by polymerization based on a complementary template (in vivo or in vitro), reproduction in a recombinant cell or system, and chemical synthesis. In some embodiments, a nucleic acid is at least 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 20, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 600, 700, 800, 900, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000 or more residues long. In some embodiments, nucleic acids may have a length from about 2 to about 5000 nts, about 10 to about 100 nts, about 50 to about 150 nts, about 100 to about 200 nts, about 150 to about 250 nts, about 200 to about 300 nts, about 250 to about 350 nts, about 300 to about 500 nts, about 10 to about 1000 nts, about 50 to about 1000 nts, about 100 to about 1000 nts, about 1000 to about 2000 nts, about 2000 to about 3000 nts, about 3000 to about 4000 nts, about 4000 to about 5000 nts, or any range therebetween. In some embodiments, a nucleic acid is partly or wholly single stranded; in some embodiments, a nucleic acid is partly or wholly double stranded. In some embodiments a nucleic acid has a nucleotide sequence comprising at least one element that encodes, or is the complement of a sequence that encodes, a polypeptide. In some embodiments, a nucleic acid has enzymatic activity.
In some embodiments, a DNA-targeting moiety comprises or is nucleic acid.
In some embodiments, a nucleic acid that may be included in a moiety may be or comprise DNA, RNA, and/or an artificial or synthetic nucleic acid or nucleic acid analog or mimic. For example, in some embodiments, a nucleic acid may be or include one or more of genomic DNA
(gDNA), complementary 5 DNA (cDNA), a peptide nucleic acid (PNA), a peptide-nucleic acid mixmer, a peptide- oligonucleotide conjugate, a locked nucleic acid (LNA), a bridged nucleic acid (BNA), a polyamide, a triplex- forming oligonucleotide, an antisense oligonucleotide, tRNA, mRNA, rRNA, miRNA, gRNA, siRNA or other RNAi molecule (e.g., that targets a non-coding RNA as described herein and/or that targets an expression product of a particular gene associated with a targeted genomic complex as described herein), etc. A
10 nucleic acid sequence may include modified oligonucleotides (e.g., chemical modifications, such as modifications that alter backbone linkages, sugar molecules, and/or nucleic acid bases) and/or artificial nucleic acids. In some embodiments, a nucleic acid sequence includes, but is not limited to, genomic DNA, cDNA, peptide nucleic acids (PNA) or peptide oligonucleotide conjugates, locked nucleic acids (LNA), bridged nucleic acids (BNA), polyamides, triplex forming oligonucleotides, modified DNA, 15 antisense DNA oligonucleotides, tRNA, mRNA, rRNA, modified RNA, miRNA, gRNA, and siRNA or other RNA or DNA molecules. In some embodiments, a nucleic acid may include one or more residues that is not a naturally occurring DNA or RNA residue, may include one or more linkages that is/are not phosphodiester bonds (e.g., that may be, for example, phosphorothioate bonds, etc.), and/or may include one or more modifications such as, for example, a 2'0 modification such as 2'-0MeP. A variety of 20 nucleic acid structures useful in preparing synthetic nucleic acids is known in the art (see, for example, W02017/0628621 and W02014/012081) those skilled in the art will appreciate that these may be utilized in accordance with the present disclosure.
In some embodiments, a nucleic acid described herein comprises one or more nucleoside analogs.
In some embodiments, a nucleic acid sequence may include in addition or as an alternative to one or more 25 natural nucleosides, e.g., purines or pyrimidines, e.g., adenine, cytosine, guanine, thymine, and uracil, one or more nucleoside analogs. In some embodiments, a nucleic acid sequence includes one or more nucleoside analogs. A nucleoside analog may include, but is not limited to, a nucleoside analog, such as 5-fluorouracil; 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 4-methylbenzimidazole, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethy1-2-thiouridine, 5-30 carboxymethylaminomethyluracil, dihydrouracil, dihydrouridine, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethy1-2-thiouracil, beta-D-mannosylqueosine, 5'-methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v), 5-methy1-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w, 2,6-diaminopurine, 3-nitropyrrole, inosine, thiouridine, queuosine, wyosine, diaminopurine, isoguanine, isocytosine, diaminopyrimidine, 2,4-difluorotoluene, isoquinoline, pyrrolo[2,3-13]pyridine, and any others that can base pair with a purine or a pyrimidine side chain.
CRISPR/Cas Domains In some embodiments, a DNA-targeting moiety is or comprises a CRISPR/Cas molecule. A
CRISPR/Cas molecule comprises a protein involved in the clustered regulatory interspaced short palindromic repeat (CRISPR) system, e.g., a Cas protein, and optionally a guide RNA, e.g., single guide RNA (sgRNA). In some embodiments, the gRNA comprised by the CRISPR/Cas molecule is noncovalently bound by the CRISPR/Cas protein.
CRISPR systems are adaptive defense systems originally discovered in bacteria and archaea.
CRISPR systems use RNA-guided nucleases termed CRISPR-associated or "Cas"
endonucleases (e. g., Cas9 or Cpfl) to cleave foreign DNA. For example, in a typical CRISPR/Cas system, an endonuclease is directed to a target nucleotide sequence (e. g., a site in the genome that is to be sequence-edited) by sequence-specific, non-coding "guide RNAs" that target single- or double-stranded DNA sequences.
Three classes (I-III) of CRISPR systems have been identified. The class II
CRISPR systems use a single Cas endonuclease (rather than multiple Cas proteins). One class II CRISPR
system includes a type II Cas endonuclease such as Cas9, a CRISPR RNA ("crRNA"), and a trans-activating crRNA ("tracrRNA").
The crRNA contains a "guide RNA", typically about 20-nucleotide RNA sequence that corresponds to a target DNA sequence. crRNA also contains a region that binds to the tracrRNA
to form a partially double-stranded structure which is cleaved by RNase III, resulting in a crRNA/tracrRNA hybrid. A
.. crRNA/tracrRNA hybrid then directs Cas9 endonuclease to recognize and cleave a target DNA sequence.
A target DNA sequence must generally be adjacent to a "protospacer adjacent motif' ("PAM") that is specific for a given Cas endonuclease; however, PAM sequences appear throughout a given genome.
CRISPR endonucleases identified from various prokaryotic species have unique PAM sequence requirements; examples of PAM sequences include 5'-NGG (Streptococcus pyogenes), 5'-NNAGAA
(Streptococcus thermophilus CRISPR1), 5' -NGGNG (Streptococcus thermophilus CRISPR3), and 5'-NNNGATT (Neisseria meningiditis). Some endonucleases, e.g., Cas9 endonucleases, are associated with G-rich PAM sites, e. g., 5'-NGG, and perform blunt-end cleaving of the target DNA at a location 3 nucleotides upstream from (5' from) the PAM site. Another class II CRISPR
system includes the type V
endonuclease Cpfl, which is smaller than Cas9; examples include AsCpfl (from Acidaminococcus sp.) and LbCpfl (from Lachnospiraceae sp.). Cpfl-associated CRISPR arrays are processed into mature crRNAs without the requirement of a tracrRNA; in other words, a Cpfl system requires only Cpfl nuclease and a crRNA to cleave a target DNA sequence. Cpfl endonucleases, are associated with T-rich PAM sites, e. g., 5'-TTN. Cpfl can also recognize a 5'-CTA PAM motif. Cpfl cleaves a target DNA by introducing an offset or staggered double-strand break with a 4- or 5-nucleotide 5' overhang, for example, cleaving a target DNA with a 5-nucleotide offset or staggered cut located 18 nucleotides downstream from (3' from) from a PAM site on the coding strand and 23 nucleotides downstream from the PAM site on the complimentary strand; the 5-nucleotide overhang that results from such offset cleavage allows more precise genome editing by DNA insertion by homologous recombination than by insertion at blunt-end cleaved DNA. See, e.g., Zetsche et al. (2015) Cell, 163:759 ¨ 771.
A variety of CRISPR associated (Cas) genes or proteins can be used in the technologies provided by the present disclosure and the choice of Cas protein will depend upon the particular conditions of the method. Specific examples of Cas proteins include class II systems including Casl, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9, Cas10, Cpfl, C2C1, or C2C3. In some embodiments, a Cas protein, e.g., a Cas9 protein, may be from any of a variety of prokaryotic species. In some embodiments a particular Cas protein, e.g., a particular Cas9 protein, is selected to recognize a particular protospacer-adjacent motif (PAM) sequence. In some embodiments, a DNA-targeting moiety includes a sequence targeting polypeptide, such as a Cas protein, e.g., Cas9. In certain embodiments a Cas protein, e.g., a Cas9 protein, may be obtained from a bacteria or archaea or synthesized using known methods.
In certain embodiments, a Cas protein may be from a gram-positive bacterium or a gram-negative bacterium. In certain embodiments, a Cas protein may be from a Streptococcus (e.g., S. pyogenes, or a S. thermophilus), a Francisella (e.g., an F. novicida), a Staphylococcus (e.g., an S. aureus), an Acidaminococcus (e.g., an Acidaminococcus sp. BV3L6), a Neisseria (e.g., an N. meningitidis), a Cryptococcus, a Corynebacterium, a Haemophilus, a Eubacterium, a Pasteurella, a Prevotella, a Veillonella, or a Marinobacter.
.. In some embodiments, a Cas protein requires a protospacer adjacent motif (PAM) to be present in or adjacent to a target DNA sequence for the Cas protein to bind and/or function.
In some embodiments, the PAM is or comprises, from 5' to 3', NGG, YG, NNGRRT, NNNRRT, NGA, TYCV, TATV, NTTN, or NNNGATT, where N stands for any nucleotide, Y stands for C or T, R stands for A or G, and V stands for A or C or G. In some embodiments, a Cas protein is a protein listed in Table 1. In some embodiments, a Cas protein comprises one or more mutations altering its PAM.
In some embodiments, a Cas protein comprises E1369R, E1449H, and R1556A mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a Cas protein comprises E782K, N968K, and R1015H mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a Cas protein comprises D1135V, R1335Q, and T1337R mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a Cas protein comprises S542R and K607R mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a Cas protein comprises S542R, K548V, and N552R mutations or analogous substitutions to the amino acids corresponding to said positions.
Table 1 Name Enzym Species # of PAM Mutations to alter Mutations to make AAs PAM recognition catalytically dead FnCas Cas9 Francisella 1629 5'-NGG- Wt 9 novicida 3' FnCas Cas9 Francisella 1629 5'-YG-3' E1369R/E1449H/R1 D11A/H969A/N995A
9 RHA novicida 556A
SaCas Cas9 Staphylococc 1053 5'- Wt D1OA/H557A
9 us aureus NNGRR
T-3' SaCas Cas9 Staphylococc 1053 5'- E782K/N968K/R10 D1OA/H557A
9 us aureus NNNRR 15H
KKH T-3' SpCas Cas9 Streptococcu 1368 5'-NGG- Wt 9 s pyogenes 3' N863A
SpCas Cas9 Streptococcu 1368 5'-NGA- D1135V/R1335Q/T D1OA/D839A/H840A/
9 VQR s pyogenes 3' 1337R N863A
AsCpf Cpfl Acidaminoco 1307 5'-TYCV- S542R/K607R E993A
1 RR ccus sp. 3' AsCpf Cpfl Acidaminoco 1307 5'-TATV- S542R/K548V/N552 E993A
1 RVR ccus sp. 3' FnCpf Cpfl Francisella 1300 5'-NTTN- Wt 1 novicida 3' A
NmCa Cas9 Neisseria 1082 5'- Wt s9 meningitidis NNNGA N611A
TT-3' In some embodiments, the Cas protein is modified to deactivate the nuclease, e.g., nuclease deficient Cas9. Whereas wild-type Cas9 generates double-strand breaks (DSBs) at specific DNA
sequences targeted by a gRNA, a number of CRISPR endonucleases having modified functionalities are available, for example: a "nickase" version of Cas9 generates only a single-strand break; a catalytically inactive Cas9 ("dCas9") does not cut target DNA. In some embodiments, dCas9 binding to a DNA
sequence may interfere with transcription at that site by steric hindrance. In some embodiments, a DNA-targeting moiety is or comprises a catalytically inactive Cas9, e.g., dCas9.
In some embodiments, a DNA-targeting moiety is or comprises a catalytically inactive mutant Cas9, e.g., Cas9m4. Many catalytically inactive Cas9 proteins are known in the art. In some embodiments, dCas9 comprises mutations in each endonuclease domain of the Cas protein, e.g., DlOA and H840A
mutations.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a DllA
mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a H969A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a N995A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises D11A, H969A, and N995A mutations or analogous substitutions to the amino acids corresponding to said positions.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a DlOA
mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a H557A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises DlOA and H557A
mutations or analogous substitutions to the amino acids corresponding to said positions.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D839A
mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a H840A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a N863A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises DlOA and D839A mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises DlOA, D839A, and H840A mutations or analogous substitutions to the amino acids corresponding to said positions. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises DlOA, D839A, H840A, and N863A mutations or analogous substitutions to the amino acids corresponding to said positions.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a E993A
mutation or an analogous substitution to the amino acid corresponding to said position.
5 In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D917A
mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a E1006A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D1255A mutation or an analogous 10 substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises D917A, E1006A, and D1255A
mutations or analogous substitutions to the amino acids corresponding to said positions.
In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D16A
mutation or an analogous substitution to the amino acid corresponding to said position. In some 15 embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a D587A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a H588A mutation or an analogous substitution to the amino acid corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises a N611A mutation or an analogous substitution to the amino acid 20 corresponding to said position. In some embodiments, a catalytically inactive Cas9 protein, e.g., dCas9, comprises D16A, D587A, H588A, and N611A mutations or analogous substitutions to the amino acids corresponding to said positions.
In another aspect, the disclosure is directed to an expression repressor or a polypeptide comprising one or more (e.g., one) DNA-targeting moiety and one or more repressor domain, wherein the 25 one or more DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a Cas protein, e.g., catalytically inactive Cas9 protein, e.g., dCas9, e.g., dCas9m4, or a functional variant or fragment thereof. In some embodiments, dCas9 comprises an amino acid sequence of SEQ ID
NO: 46 or 88:
DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEA
TRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVA
NQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDL
AEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIK
RYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEE
LLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPL
ARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFT
VYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGV
EDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMK
QLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSR
ERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD INRLSDYDVAAIV
PQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAER
GGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDF
QFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKAT
AKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWD KGRDFATVRKVLSMPQVNIV KKTE
V QTGGFSKESILPKRNS D KLIARKKDWDPKKYGGFD SPTVAYSVLVVAKVEKGKSKKLKSVKEL
LGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLASAGELQKGNELALP
SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAY
NKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRID
LSQLGGD (SEQ ID NO: 46) AKRNYILGLAIGIT SVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRS KRGARRLKRRR
RHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLS QKLSEEEFSAALLHLAKRRGVHNVNEVE
EDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKA
YHQLD QSFIDTYIDLLETRRTYYEGPGEGS PFGWKDIKEWYEMLMGHCTYFPEELRSV KYAYNA
DLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTG
KPEFTNLKVYHDIKDITARKEIIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKG
YTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFI
QS IKVINAIIKKYGLPND IIIELAREKNS KDAQKMINEMQKRNRQTNERIEEIIRTTGKENAKYLIEK
IKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDAIIPRS VS FDNS FNNKVLVKQEENSKKGNRTPF
QYLSS SD S KISYETFKKHILNLAKGKGRIS KTKKEYLLEERDINRFSV QKD FINRNLVDTRYATRG
LMNLLRS YFRVNNLDVKV KS INGGFTS FLRRKWKFKKERNKGYKHHAEDALIIANADFIFKEWK
KLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQIKHIKDFKDYKYSHRVDKKPNRKLI
NDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQY
GDEKNPLYKYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKP
YRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYKNDLIKIN
GELYRVIGVNNDLLNRIEVNMIDITYREYLENMNDKRPPHIIKTIASKTQSIKKYSTDILGNLYEVK
SKKHPQIIKKG (SEQ ID NO: 88) In some embodiments, the dCas9 is encoded by a nucleic acid sequence of SEQ ID
NO: 45 or 89:
GACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCA
CCGACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAG
CATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACC
CGGCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGC
AGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGA
GAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTG
GACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGG
ACAGCACCGACAAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTC
CGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGT
TCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGG
CGTGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTG
ATCGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCC
TGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCT
GAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTAC
GCCGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCG
GGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAG
CACCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACA
AGGAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAG
CCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAG
CTGCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCA
GCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTC
TACCCCTTCCTGAAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTA
CTACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAG
GAGACCATCACCCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCT
TCATCGAGCGGATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCA
CAGCCTGCTGTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCG
AGGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCT
GTTCAAGACCAACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATC
GAGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCA
CCTACCACGACCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGA
GGACATCCTGGAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAG
GAGCGGCTGAAAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGC
GGCGGTACACCGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCA
GAGCGGCAAGACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGC
AGCTGATCCACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGG
CCAGGGCGACAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAG
GGCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGC
CCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGA
ACAGCCGGGAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCC
TGAAGGAGCACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCT
GCAGAACGGCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTAC
GACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGC
TGACCCGGAGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGA
AGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTT
CGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATC
AAGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCC
GGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCT
GAAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATC
AACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCA
AGAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCG
GAAGATGATCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTAC
AGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGC
GGCCCCTGATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTT
CGCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTG
CAGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCG
CCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTA
CAGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAA
GGAGCTGCTGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTC
CTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACA
GCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAA
GGGCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACG
AGAAGCTGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACA
AGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGC
CGACGCCAACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGG
GAGCAGGCCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTT
CAAGTACTTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGAC
GCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCT
GGGCGGCGAC (SEQ ID NO: 45) GCCAAGCGGAACTACATCCTGGGCCTGGCCATCGGCATCACCAGCGTGGGCTACGGCATCAT
CGACTACGAGACCCGGGACGTGATCGACGCCGGCGTGCGGCTGTTCAAGGAGGCCAACGTG
GAGAACAACGAGGGCCGGCGGAGCAAGCGGGGCGCCCGGCGGCTGAAGCGGCGGCGGCGG
CACCGGATCCAGCGGGTGAAGAAGCTGCTGTTCGACTACAACCTGCTGACCGACCACAGCG
AGCTGAGCGGCATCAACCCCTACGAGGCCCGGGTGAAGGGCCTGAGCCAGAAGCTGAGCGA
GGAGGAGTTCAGCGCCGCCCTGCTGCACCTGGCCAAGCGGCGGGGCGTGCACAACGTGAAC
GAGGTGGAGGAGGACACCGGCAACGAGCTGAGCACCAAGGAGCAGATCAGCCGGAACAGC
AAGGCCCTGGAGGAGAAGTACGTGGCCGAGCTGCAGCTGGAGCGGCTGAAGAAGGACGGC
GAGGTGCGGGGCAGCATCAACCGGTTCAAGACCAGCGACTACGTGAAGGAGGCCAAGCAGC
TGCTGAAGGTGCAGAAGGCCTACCACCAGCTGGACCAGAGCTTCATCGACACCTACATCGA
CCTGCTGGAGACCCGGCGGACCTACTACGAGGGCCCCGGCGAGGGCAGCCCCTTCGGCTGG
AAGGACATCAAGGAGTGGTACGAGATGCTGATGGGCCACTGCACCTACTTCCCCGAGGAGC
TGCGGAGCGTGAAGTACGCCTACAACGCCGACCTGTACAACGCCCTGAACGACCTGAACAA
CCTGGTGATCACCCGGGACGAGAACGAGAAGCTGGAGTACTACGAGAAGTTCCAGATCATC
GAGAACGTGTTCAAGCAGAAGAAGAAGCCCACCCTGAAGCAGATCGCCAAGGAGATCCTGG
TGAACGAGGAGGACATCAAGGGCTACCGGGTGACCAGCACCGGCAAGCCCGAGTTCACCAA
CCTGAAGGTGTACCACGACATCAAGGACATCACCGCCCGGAAGGAGATCATCGAGAACGCC
GAGCTGCTGGACCAGATCGCCAAGATCCTGACCATCTACCAGAGCAGCGAGGACATCCAGG
AGGAGCTGACCAACCTGAACAGCGAGCTGACCCAGGAGGAGATCGAGCAGATCAGCAACCT
GAAGGGCTACACCGGCACCCACAACCTGAGCCTGAAGGCCATCAACCTGATCCTGGACGAG
CTGTGGCACACCAACGACAACCAGATCGCCATCTTCAACCGGCTGAAGCTGGTGCCCAAGA
AGGTGGACCTGAGCCAGCAGAAGGAGATCCCCACCACCCTGGTGGACGACTTCATCCTGAG
CCCCGTGGTGAAGCGGAGCTTCATCCAGAGCATCAAGGTGATCAACGCCATCATCAAGAAG
TACGGCCTGCCCAACGACATCATCATCGAGCTGGCCCGGGAGAAGAACAGCAAGGACGCCC
AGAAGATGATCAACGAGATGCAGAAGCGGAACCGGCAGACCAACGAGCGGATCGAGGAGA
TCATCCGGACCACCGGCAAGGAGAACGCCAAGTACCTGATCGAGAAGATCAAGCTGCACGA
CATGCAGGAGGGCAAGTGCCTGTACAGCCTGGAGGCCATCCCCCTGGAGGACCTGCTGAAC
AACCCCTTCAACTACGAGGTGGACGCCATCATCCCCCGGAGCGTGAGCTTCGACAACAGCTT
CAACAACAAGGTGCTGGTGAAGCAGGAGGAGAACAGCAAGAAGGGCAACCGGACCCCCTT
CCAGTACCTGAGCAGCAGCGACAGCAAGATCAGCTACGAGACCTTCAAGAAGCACATCCTG
AACCTGGCCAAGGGCAAGGGCCGGATCAGCAAGACCAAGAAGGAGTACCTGCTGGAGGAG
CGGGACATCAACCGGTTCAGCGTGCAGAAGGACTTCATCAACCGGAACCTGGTGGACACCC
GGTACGCCACCCGGGGCCTGATGAACCTGCTGCGGAGCTACTTCCGGGTGAACAACCTGGA
CGTGAAGGTGaaatccATCAACGGCGGCTTCACCAGCTTCCTGCGGCGGAAGTGGAAGTTCAAG
AAGGAGCGGAACAAGGGCTACAAGCACCACGCCGAGGACGCCCTGATCATCGCCAACGCCG
ACTTCATCTTCAAGGAGTGGAAGAAGCTGGACAAGGCCAAGAAGGTGATGGAGAACCAGAT
GTTCGAGGAGAAGCAGGCCGAGAGCATGCCCGAGATCGAGACCGAGCAGGAGTACAAGGA
GATCTTCATCACCCCCCACCAGATCAAGCACATCAAGGACTTCAAGGACTACAAGTACAGCC
ACCGGGTGGACAAGAAGCCCAACCGGAAGCTGATCAACGACACCCTGTACAGCACCCGGAA
GGACGACAAGGGCAACACCCTGATCGTGAACAACCTGAACGGCCTGTACGACAAGGACAAC
GACAAGCTGAAGAAGCTGATCAACAAGAGCCCCGAGAAGCTGCTGATGTACCACCACGACC
CCCAGACCTACCAGAAGCTGAAGCTGATCATGGAGCAGTACGGCGACGAGAAGAACCCCCT
GTACAAGTACTACGAGGAGACCGGCAACTACCTGACCAAGTACAGCAAGAAGGACAACGGC
CCCGTGATCAAGAAGATCAAGTACTACGGCAACAAGCTGAACGCCCACCTGGACATCACCG
ACGACTACCCCAACAGCCGGAACAAGGTGGTGAAGCTGAGCCTGAAGCCCTACCGGTTCGA
CGTGTACCTGGACAACGGCGTGTACAAGTTCGTGACCGTGAAGAACCTGGACGTGATCAAG
AAGGAGAACTACTACGAGGTGAACAGCAAGTGCTACGAGGAGGCCAAGAAGCTGAAGAAG
ATCAGCAACCAGGCCGAGTTCATCGCCAGCTTCTACAAGAACGACCTGATCAAGATCAACG
GCGAGCTGTACCGGGTGATCGGCGTGAACAACGACCTGCTGAACCGGATCGAGGTGAACAT
GATCGACATCACCTACCGGGAGTACCTGGAGAACATGAACGACAAGCGGCCCCCCCACATC
ATCAAGACCATCGCCAGCAAGACCCAGAGCATCAAGAAGTACAGCACCGACATCCTGGGCA
ACCTGTACGAGGTGaaatccAAGAAGCACCCCCAGATCATCAAGAAGGGC (SEQ ID NO: 89) Guide RNA (gRNA) In some embodiments, a DNA-targeting moiety may comprise a Cas molecule comprising or linked (e.g., covalently) to a gRNA. A gRNA is a short synthetic RNA composed of a "scaffold" sequence necessary for Cas-protein binding and a user-defined ¨20 nucleotide targeting sequence for a genomic target. In practice, guide RNA sequences are generally designed to have a length of between 17 ¨ 24 nucleotides (e.g., 19, 20, or 21 nucleotides) and be complementary to the targeted nucleic acid sequence. Custom gRNA generators and algorithms are available commercially for use in the design of effective guide RNAs. Gene editing has also been achieved using a chimeric "single guide RNA"
("sgRNA"), an engineered (synthetic) single RNA molecule that mimics a naturally occurring crRNA-tracrRNA complex and contains both a tracrRNA (for binding the nuclease) and at least one crRNA
(to guide the nuclease to the sequence targeted for editing). Chemically modified sgRNAs have also been demonstrated to be effective for use with Cas proteins; see, for example, Hendel et al. (2015) Nature Biotechnol., 985 ¨991.
In some embodiments, a gRNA comprises a nucleic acid sequence that is complementary to a DNA sequence associated with a target gene. In some embodiments, the DNA
sequence is, comprises, or overlaps an expression control element that is operably linked to the target gene. In some embodiments, a gRNA comprises a nucleic acid sequence that is at least 90, 95, 99, or 100%
complementary to a DNA
sequence associated with a target gene. In some embodiments, a gRNA for use with a DNA-targeting moiety that comprises a Cas molecule is an sgRNA.
In some embodiments, a gRNA for use with a CRISPR/Cas molecule specifically binds a target sequence associated with I3-2-microglobulin expression. Such a gRNA may comprise a target-binding sequence selected from:
GD-28228: TCTCCTTGGTGGCCCGCCGT (SEQ ID NO: 1), GD-28229: GTCCCAAAGGCGCGGCGCTG (SEQ ID NO: 2), GD-28171: CCCTGCTCCCCGCCGAAAGGG (SEQ ID NO: 3), GD-28172: CTCTGGCTCCCCCAGCGCAGC (SEQ ID NO: 4), or GD-28173: GTGAACGCGTGGAGGGGCGCT (SEQ ID NO: 5).
In some embodiments, a gRNA for use with a CRISPR/Cas domain specifically binds a target sequence associated with CTCF. In some embodiments, a gRNA for use with a CRISPR/Cas domain specifically binds a target sequence associated with the promoter. In some embodiments the gRNA binds a target sequence listed in Table 3.
Table 3: Exemplary gRNA sequences Guide Target Target sequence Genomic SEQ ID NO:
Coordinates GD- MYC chr8:128746082- 11 GD- MYC chr8:128746177- 12 GD- I32M chr15:45003632- 13 GD- I32M chr15:45003545- 14 GD- HSPA1B chr6:31795352- 17 GD- HSPA1B chr6:31795429- 18 GD- GATA1 chrX:48641126- 19 GD- GATA1 chrX:48641245- 20 GD- I32M GGCGCGCACCCCAGATCGGA chr15 :45003481- 21 29499 promoter 45003503 In some embodiments, an expression repressor system comprises a first expression repressor comprising a first DNA-targeting moiety and a second expression repressor comprising a second DNA-targeting moiety, wherein the first DNA-targeting moiety comprises or is a first CRISPR/Cas molecule and the second DNA-targeting moiety comprises or is a second CRISPR/Cas molecule. In some embodiments, the first CRISPR/Cas molecule comprises a first CRISPR/Cas protein and first guide RNA, and the second CRISPR/Cas molecule comprises a second CRISPR/Cas protein and a second guide RNA.
In some embodiments, the first CRISPR/Cas protein does not appreciably bind (e.g., does not bind) the second guide RNA, e.g., binds with a KD of at least 10, 20, 50, 100, 1000, or 10,000 nM, and the second .. CRISPR/Cas protein does not appreciably bind (e.g., does not bind) the first guide RNA, e.g., binds with a KD of at least 10, 20, 50, 100, 1000, or 10,000 nM.
TAL effector domains In some embodiments, a DNA-targeting moiety is or comprises a TAL effector molecule. A TAL
effector molecule, e.g., a TAL effector molecule that specifically binds a DNA
sequence, comprises a plurality of TAL effector domains or fragments thereof, and optionally one or more additional portions of naturally occurring TAL effectors (e.g., N- and/or C-terminal of the plurality of TAL effector domains).
Many TAL effectors are known to those of skill in the art and are commercially available, e.g., from Thermo Fisher Scientific.
TALEs are natural effector proteins secreted by numerous species of bacterial pathogens including the plant pathogen Xanthomonas which modulates gene expression in host plants and facilitates bacterial colonization and survival. The specific binding of TAL effectors is based on a central repeat domain of tandemly arranged nearly identical repeats of typically 33 or 34 amino acids (the repeat-variable di-residues, RVD domain).
Members of the TAL effectors family differ mainly in the number and order of their repeats. The number of repeats ranges from 1.5 to 33.5 repeats and the C-terminal repeat is usually shorter in length (e.g., about 20 amino acids) and is generally referred to as a "half-repeat".
Each repeat of the TAL
effector feature a one-repeat-to-one-base-pair correlation with different repeat types exhibiting different base-pair specificity (one repeat recognizes one base-pair on the target gene sequence). Generally, the smaller the number of repeats, the weaker the protein-DNA interactions. A
number of 6.5 repeats has been shown to be sufficient to activate transcription of a reporter gene (Scholze et al., 2010).
Repeat to repeat variations occur predominantly at amino acid positions 12 and 13, which have therefore been termed "hypervariable" and which are responsible for the specificity of the interaction with the target DNA promoter sequence, as shown in Table 2 listing exemplary repeat variable di-residues (RVD) and their correspondence to nucleic acid base targets.
Table 2 ¨ RVDs and Nucleic Acid Base Specificity Target Possible RVD Amino Acid Combinations A NI NN CI HI KI
NN GN SN VN LN DN QN EN HN RH NK AN FN
HD RD KD ND AD
NG HG VG IG EG MG YG AA EP VA QG KG RG
Accordingly, it is possible to modify the repeats of a TAL effector to target specific DNA sequences.
Further studies have shown that the RVD NK can target G. Target sites of TAL
effectors also tend to include a T flanking the 5' base targeted by the first repeat, but the exact mechanism of this recognition is not known. More than 113 TAL effector sequences are known to date. Non-limiting examples of TAL
effectors from Xanthomonas include, Hax2, Hax3, Hax4, AvrXa7, AvrXa10 and AvrB
s3.
Accordingly, the TAL effector domain of the TAL effector molecule of the present invention may be derived from a TAL effector from any bacterial species (e.g., Xanthomonas species such as the African strain of Xanthomonas myzae pv. Oiyzae (Yu et al. 2011), Xanthomonas campestris pv. raphani strain 756C and Xanthomonas myzae pv. olyzico/astrain BL5256 (Bogdanove et al. 2011).
As used herein, the TAL effector domain in accordance with the present invention comprises an RVD
domain as well as flanking sequence(s) (sequences on the N-terminal and/or C-terminal side of the RVD domain) also from the naturally occurring TAL effector. It may comprise more or fewer repeats than the RVD of the naturally occurring TAL effector. The TAL effector molecule of the present invention is designed to target a given DNA sequence based on the above code and others known in the art. The number of TAL
effector domains (e.g., repeats (monomers or modules)) and their specific sequence are selected based on the desired DNA target sequence. For example, TAL effector domains, e.g., repeats, may be removed or added in order to suit a specific target sequence. In an embodiment, the TAL
effector molecule of the present invention comprises between 6.5 and 33.5 TAL effector domains, e.g., repeats. In an embodiment, TAL effector molecule of the present invention comprises between 8 and 33.5 TAL effector domains, e.g., repeats, e.g., between 10 and 25 TAL effector domains, e.g., repeats, e.g., between 10 and 14 TAL
effector domains, e.g., repeats.
In some embodiments, the TAL effector molecule comprises TAL effector domains that correspond to a perfect match to the DNA target sequence. In some embodiments, a mismatch between a repeat and a target base-pair on the DNA target sequence is permitted as along as it allows for the function of the expression repression system, e.g., the expression repressor comprising the TAL effector molecule. In general, TALE binding is inversely correlated with the number of mismatches. In some embodiments, the TAL effector molecule of an expression repressor of the present invention comprises no more than 7 mismatches, 6 mismatches, 5 mismatches, 4 mismatches, 3 mismatches, 2 mismatches, or 1 mismatch, and optionally no mismatch, with the target DNA sequence. Without wishing to be bound by theory, in general the smaller the number of TAL effector domains in the TAL
effector molecule, the smaller the number of mismatches will be tolerated and still allow for the function of the expression repression system, e.g., the expression repressor comprising the TAL effector molecule. The binding affinity is thought to depend on the sum of matching repeat-DNA combinations.
For example, TAL
effector molecules having 25 TAL effector domains or more may be able to tolerate up to 7 mismatches.
In addition to the TAL effector domains, the TAL effector molecule of the present invention may comprise additional sequences derived from a naturally occurring TAL effector.
The length of the C-terminal and/or N-terminal sequence(s) included on each side of the TAL
effector domain portion of the TAL effector molecule can vary and be selected by one skilled in the art, for example based on the studies of Zhang et al. (2011). Zhang et al., have characterized a number of C-terminal and N-terminal truncation mutants in Hax3 derived TAL-effector based proteins and have identified key elements, which contribute to optimal binding to the target sequence and thus activation of transcription. Generally, it was found that transcriptional activity is inversely correlated with the length of N-terminus. Regarding the C-terminus, an important element for DNA binding residues within the first 68 amino acids of the Hax 3 sequence was identified. Accordingly, in some embodiments, the first 68 amino acids on the C-terminal side of the TAL
effector domains of the naturally occurring TAL effector is included in the TAL effector molecule of an expression repressor of the present invention. Accordingly, in an embodiment, a TAL effector molecule of the present invention comprises 1) one or more TAL effector domains derived from a naturally occurring TAL effector; 2) at least 70, 80, 90, 100, 110, 120, 130, 140, 150, 170, 180, 190, 200, 220, 230, 240, 250, 260, 270, 280 or more amino acids from the naturally occurring TAL
effector on the N-terminal side of the TAL effector domains; and/or 3) at least 68, 80, 90, 100, 110, 120, 130, 140, 150, 170, 180, 190, 200, 220, 230, 240, 250, 260 or more amino acids from the naturally occurring TAL effector on the C-terminal side of the TAL effector.
Zn finger domains In some embodiments, a DNA-targeting moiety is or comprises a Zn finger domain. A Zn finger domain comprises a Zn finger protein, e.g., a naturally occurring Zn finger protein or engineered Zn finger protein, or fragment thereof. Many Zn finger proteins are known to those of skill in the art and are commercially available, e.g., from Sigma-Aldrich.
In some embodiments, a Zn finger domain comprises a non-naturally occurring Zn finger protein that is engineered to bind to a target DNA sequence of choice. See, for example, Beerli, et al. (2002) Nature Biotechnol. 20:135-141; Pabo, et al. (2001) Ann. Rev. Biochem. 70:313-340; Isalan, et al. (2001) Nature Biotechnol. 19:656-660; Segal, et al. (2001) Curr. Opin. Biotechnol.
12:632-637; Choo, et al.
(2000) Curr. Opin. Struct. Biol. 10:411-416; U.S. Pat. Nos. 6,453,242;
6,534,261; 6,599,692; 6,503,717;
6,689,558; 7,030,215; 6,794,136; 7,067,317; 7,262,054; 7,070,934; 7,361,635;
7,253,273; and U.S. Patent Publication Nos. 2005/0064474; 2007/0218528; 2005/0267061, all incorporated herein by reference in their entireties.
An engineered Zn finger protein may have a novel binding specificity, compared to a naturally .. occurring Zn finger protein. Engineering methods include, but are not limited to, rational design and various types of selection. Rational design includes, for example, using databases comprising triplet (or quadruplet) nucleotide sequences and individual Zn finger amino acid sequences, in which each triplet or quadruplet nucleotide sequence is associated with one or more amino acid sequences of zinc fingers which bind the particular triplet or quadruplet sequence. See, for example, U.S. Pat. Nos. 6,453,242 and 6,534,261, incorporated by reference herein in their entireties.
Exemplary selection methods, including phage display and two-hybrid systems, are disclosed in U.S. Pat. Nos. 5,789,538; 5,925,523; 6,007,988; 6,013,453; 6,410,248;
6,140,466; 6,200,759; and 6,242,568; as well as International Patent Publication Nos. WO 98/37186; WO
98/53057; WO 00/27878;
and WO 01/88197 and GB 2,338,237. In addition, enhancement of binding specificity for zinc finger .. proteins has been described, for example, in International Patent Publication No. WO 02/077227.
In addition, as disclosed in these and other references, zinc finger domains and/or multi-fingered zinc finger proteins may be linked together using any suitable linker sequences, including for example, linkers of 5 or more amino acids in length. See, also, U.S. Pat. Nos.
6,479,626; 6,903,185; and 7,153,949 for exemplary linker sequences 6 or more amino acids in length. The proteins described herein may .. include any combination of suitable linkers between the individual zinc fingers of the protein. In addition, enhancement of binding specificity for zinc finger binding domains has been described, for example, in co-owned International Patent Publication No. WO 02/077227.
Zn finger proteins and methods for design and construction of fusion proteins (and polynucleotides encoding same) are known to those of skill in the art and described in detail in U.S. Pat.
Nos. 6,140,0815; 789,538; 6,453,242; 6,534,261; 5,925,523; 6,007,988;
6,013,453; and 6,200,759;
International Patent Publication Nos. WO 95/19431; WO 96/06166; WO 98/53057;
WO 98/54311; WO
00/27878; WO 01/60970; WO 01/88197; WO 02/099084; WO 98/53058; WO 98/53059; WO
98/53060;
WO 02/016536; and WO 03/016496.
In addition, as disclosed in these and other references, Zn finger proteins and/or multi-fingered Zn finger proteins may be linked together, e.g., as a fusion protein, using any suitable linker sequences, including for example, linkers of 5 or more amino acids in length. See, also, U.S. Pat. Nos. 6,479,626;
6,903,185; and 7,153,949 for exemplary linker sequences 6 or more amino acids in length. The Zn finger domains described herein may include any combination of suitable linkers between the individual zinc finger proteins and/or multi-fingered Zn finger proteins of the Zn finger domain.
In certain embodiments, the DNA-targeting moiety comprises a Zn finger domain comprising an engineered zinc finger protein that binds (in a sequence-specific manner) to a target DNA sequence. In some embodiments, the Zn finger domain comprises one Zn finger protein or fragment thereof. In other embodiments, the Zn finger domain comprises a plurality of Zn finger proteins (or fragments thereof), e.g., 2, 3, 4, 5, 6 or more Zn finger proteins (and optionally no more than 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, or 2 Zn finger proteins). In some embodiments, the Zn finger domain comprises at least three Zn finger proteins. In some embodiments, the Zn finger domain comprises four, five or six fingers. In some embodiments, the Zn finger domain comprises 8, 9, 10, 11 or 12 fingers. In some embodiments, a Zn finger domain comprising three Zn finger proteins recognizes a target DNA
sequence comprising 9 or 10 nucleotides. In some embodiments, a Zn finger domain comprising four Zn finger proteins recognizes a target DNA sequence comprising 12 to 14 nucleotides. In some embodiments, a Zn finger domain comprising six Zn finger proteins recognizes a target DNA sequence comprising 18 to 21 nucleotides.
In some embodiments, a Zn finger domain comprises a two-handed Zn finger protein. Two handed zinc finger proteins are those proteins in which two clusters of zinc finger proteins are separated by intervening amino acids so that the two zinc finger domains bind to two discontinuous target DNA
sequences. An example of a two-handed type of zinc finger binding protein is SIP1, where a cluster of four zinc finger proteins is located at the amino terminus of the protein and a cluster of three Zn finger proteins is located at the carboxyl terminus (see Remade, et al. (1999) EMBO
Journal 18(18):5073-5084).
Each cluster of zinc fingers in these proteins is able to bind to a unique target sequence and the spacing between the two target sequences can comprise many nucleotides.
Nucleic acid molecule In some embodiments, a DNA-targeting moiety is or comprises a DNA-binding domain from a nuclease.
For example, the recognition sequences of homing endonucleases and meganucleases such as I-SceI, I-CeuI, PI-PspI, PI-Sce, I-SceIV, I-CsmI, I-PanI, I-SceII, I-PpoI, I-SceIII, I-CreI, I-TevI, I-TevII and I-TevIII are known. See also U.S. Pat. Nos. 5,420,032; 6,833,252; Belfort, et al. (1997) Nucleic Acids Res.
25:3379-3388; Dujon, et al. (1989) Gene 82:115-118; Perler, et al. (1994) Nucleic Acids Res. 22:1125-1127; Jasin (1996) Trends Genet. 12:224-228; Gimble, et al. (1996) J. Mol.
Biol. 263:163-180; Argast, et al. (1998) J. Mol. Biol. 280:345-353 and the New England Biolabs catalogue. In addition, the DNA-binding specificity of homing endonucleases and meganucleases can be engineered to bind non-natural target sites. See, for example, Chevalier, et al. (2002) Molec. Cell 10:895-905; Epinat, et al. (2003) Nucleic Acids Res. 31:2952-2962; Ashworth, et al. (2006) Nature 441:656-659;
Paques, et al. (2007) Current Gene Therapy 7:49-66; U.S. Patent Publication No. 2007/0117128.
In some embodiments, a DNA-targeting moiety comprises or is nucleic acid. In some embodiments, a nucleic acid that may be included in a DNA-targeting moiety, may be or comprise DNA, RNA, and/or an artificial or synthetic nucleic acid or nucleic acid analog or mimic. For example, in some embodiments, a nucleic acid may be or include one or more of genomic DNA
(gDNA), complementary DNA (cDNA), a peptide nucleic acid (PNA), a peptide- oligonucleotide conjugate, a locked nucleic acid (LNA), a bridged nucleic acid (B NA), a polyamide, a triplex- forming oligonucleotide, an antisense oligonucleotide, tRNA, mRNA, rRNA, miRNA, gRNA, siRNA or other RNAi molecule (e.g., that targets a non-coding RNA as described herein and/or that targets an expression product of a particular gene associated with a targeted genomic complex as described herein), etc. In some embodiments, a nucleic acid may include one or more residues that is not a naturally occurring DNA or RNA residue, may include one or more linkages that is/are not phosphodiester bonds (e.g., that may be, for example, phosphorothioate bonds, etc.), and/or may include one or more modifications such as, for example, a 2'0 modification such as 2'-0MeP. A variety of nucleic acid structures useful in preparing synthetic nucleic acids is known in the art (see, for example, W02017/0628621 and W02014/012081) those skilled in the art will appreciate that these may be utilized in accordance with the present disclosure.
A nucleic acid suitable for use in an expression repressor, e.g., in the DNA-targeting moiety, may include, but is not limited to, DNA, RNA, modified oligonucleotides (e.g., chemical modifications, such as modifications that alter backbone linkages, sugar molecules, and/or nucleic acid bases), and artificial nucleic acids. In some embodiments, a nucleic acid includes, but is not limited to, genomic DNA, cDNA, peptide nucleic acids (PNA) or peptide oligonucleotide conjugates, locked nucleic acids (LNA), bridged nucleic acids (BNA), polyamides, triplex forming oligonucleotides, modified DNA, antisense DNA
oligonucleotides, tRNA, mRNA, rRNA, modified RNA, miRNA, gRNA, and siRNA or other RNA or DNA molecules.
In some embodiments, a DNA-targeting moiety comprises a nucleic acid with a length from about 15-200, 20-200, 30-200, 40-200, 50-200, 60-200, 70-200, 80-200, 90-200, 100-200, 110-200, 120-200, 130-200, 140-200, 150-200, 160-200, 170-200, 180-200, 190-200, 215-190, 20-190, 30-190, 40-190, 50-190, 60-190, 70-190, 80-190, 90-190, 100-190, 110-190, 120-190, 130-190, 140-190, 150-190, 160-190, 170-190, 180-190, 15-180, 20-180, 30-180, 40-180, 50-180, 60-180, 70-180, 80-180, 90-180, 100-180, 110-180, 120-180, 130-180, 140-180, 150-180, 160-180, 170-180, 15-170, 20-170, 30-170, 40-170, 50-170, 60-170, 70-170, 80-170, 90-170, 100-170, 110-170, 120-170, 130-170, 140-170, 150-170, 160-170, 15-160, 20-160, 30-160, 40-160, 50-160, 60-160, 70-160, 80-160, 90-160, 100-160, 110-160, 120-160, 130-160, 140-160, 150-160, 215-150, 20-150, 30-150, 40-150, 50-150, 60-150, 70-150, 80-150, 90-150, 100-150, 110-150, 120-150, 130-150, 140-150, 15-140, 20-140, 30-140, 40-140, 50-140, 60-140, 70-140, 80-140, 90-140, 100-140, 110-140, 120-140, 130-140, 15-130, 20-130, 30-130, 40-130, 50-130, 60-130, 70-130, 80-130, 90-130, 100-130, 110-130, 120-130, 215-120, 20-120, 30-120, 40-120, 50-120, 60-120, 70-120, 80-120, 90-120, 100-120, 110-120, 15-110, 20-110, 30-110, 40-110, 50-110, 60-110, 70-110, 80-110, 90-110, 100-110, 15-100, 20-100, 30-100, 40-100, 50-100, 60-100, 70-100, 80-100, 90-100, 15-90, 20-90, 30-90, 40-90, 50-90, 60-90, 70-90, 80-90, 15-80, 20-80, 30-80, 40-80, 50-80, 60-80, 70-80, 15-70, 20-70, 30-70, 40-70, 50-70, 60-70, 15-60, 20-60, 30-60, 40-60, 50-60, 15-50, 20-50, 30-50, 40-50, 15-40, 20-40, 30-40, 15-30, 20-30, or 15-20 nucleotides, or any range therebetween.
Repressor Domains Expression repressors of the present disclosure comprise one or more repressor domains. A
repressor domain has one or more functionality that, when used as part of an expressor repressor or an expression repression system described herein, decreases expression of a target gene in a cell.
In some embodiments, a repressor domain comprises a histone modifying functionality, e.g., a histone methyltransferase, histone demethylase, or histone deacetylase activity. In some embodiments, a histone methyltransferase functionality comprises H3K9 targeting methyltransferase activity. In some embodiments, a histone methyltransferase functionality comprises H3K56 targeting methyltransferase activity. In some embodiments, a histone methyltransferase functionality comprises H3K27 targeting methyltransferase activity. In some embodiments, a histone methyltransferase or demethylase functionality transfers one, two, or three methyl groups. In some embodiments, a histone demethylase functionality comprises H3K4 targeting demethylase activity. In some embodiments, a repressor domain is or comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, SUV39H2, SETD8, SUV420H1, SUV420H2, or a functional variant or fragment of any thereof, e.g., a SET domain of any thereof. In some embodiments, a repressor domain is or comprises a protein chosen from KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, or a functional variant or fragment of any thereof. In some embodiments, a repressor domain is or comprises a protein chosen from HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof.
In some embodiments, a repressor domain comprises an epigenetic modifying moiety. In some embodiments, a repressor domain comprises a DNA modifying functionality, e.g., a DNA
methyltransferase. In some embodiments, a repressor domain is or comprises a protein chosen from MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, DNMT3a/3L or a functional variant or fragment of any thereof.
In some embodiments, a repressor domain comprises a transcription repressor.
In some embodiments the transcription repressor blocks recruitment of a factor that stimulates or promotes transcription, e.g., of the target gene. In some embodiments, the transcription repressor recruits a factor that inhibits transcription, e.g., of the target gene. In some embodiments, a repressor domain, e.g., transcription repressor, is or comprises a protein chosen from KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment of any thereof.
In some embodiments a repressor domain promotes epigenetic modification, e.g., directly, or indirectly. For example, a repressor domain can indirectly promote epigenetic modification by recruiting an endogenous protein that epigenetically modifies the chromatin. In some embodiments, a repressor domain can directly promote epigenetic modification by catalyzing epigenetic modification, wherein the repressor domain comprises enzymatic activity and directly places an epigenetic mark on the chromatin.
In some embodiments, a repressor domain comprises a protein having a functionality described herein. In some embodiments, a repressor domain is or comprises a protein selected from:
KRAB (e.g., as according to NP_056209.2 or the protein encoded by NM_015394.5, e.g., as according to SEQ ID NO: 61);
a SET domain (e.g., the SET domain of:
SETDB1 (e.g., as according to NP_001353347.1 or the protein encoded by NM_001366418.1);
EZH2 (e.g., as according to NP-004447.2 or the protein encoded by NM_004456.5, e.g., as according to SEQ ID NO: 65);
G9A (e.g., as according to NP_001350618.1 or the protein encoded by NM_001363689.1; e.g., as according to SEQ ID NO: 62); or SUV39H1 (e.g., as according to NP_003164.1 or the protein encoded by NM_003173.4));
histone demethylase LSD1 (e.g., as according to NP_055828.2 or the protein encoded by NM_015013.4, e.g., as according to SEQ ID NO: 64);
FOG1 (e.g., the N-terminal residues of FOG1) (e.g., as according to NP_722520.2 or the protein encoded by NM_153813.3, e.g., as according to SEQ ID NO: 66);
KAP1 (e.g., as according to NP_005753.1 or the protein encoded by NM_005762.3); or HDAC8 (e.g., as according to NP_001159890 or the protein encoded by NM_001166418, e.g., as according to SEQ ID NO: 63);
a functional fragment or variant of any thereof, or a polypeptide with a sequence that has at least 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identity to any of the above-referenced sequences. In some embodiments, a repressor domain is or comprises a protein selected from:
DNMT3A (e.g., human DNMT3A) (e.g., as according to NP_072046.2 or the protein encoded by NM_022552.4, e.g., as according to SEQ ID NO: 58);
DNMT3B (e.g., as according to or A0X21819.1 .. or the protein encoded by NM_006892.4 or I(X447429);
DNMT3L (e.g., as according to NP_787063.1 or the protein encoded by NM_175867.3);
DNMT3A/3L complex (e.g., as according to SEQ ID NO: 59 or 60), bacterial MQ1 (e.g., as according to CAA35058.1 or P15840.3, e.g., as according to SEQ ID NO: 57 or 90);
a functional fragment of any thereof, or a polypeptide with a sequence that has at least 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% identity to any of the above-referenced sequences.
.. In some embodiments, a repressor domain is or comprises a polypeptide. In some embodiments, a repressor domain has enzymatic activity.
In some embodiments, a DNA-binding domain comprises a helix-hairpin-helix (HhH) motif.
DNA-binding proteins with a HhH structural motif may be involved in non-sequence-specific DNA
.. binding that occurs via the formation of hydrogen bonds between protein backbone nitrogens and DNA
phosphate groups.
In some embodiments, a DNA-binding domain comprises a helix-loop-helix (HLH) motif. DNA-binding proteins with an HLH structural motif are transcriptional regulatory proteins and are principally related to a wide array of developmental processes. An HLH structural motif is longer, in terms of residues, than HTH or HhH motifs. Many of these proteins interact to form homo-and hetero-dimers. A
structural motif is composed of two long helix regions, with an N-terminal helix binding to DNA, while a complex region allows the protein to dimerize.
In some embodiments, a DNA-binding domain comprises a leucine zipper motif. In some transcription factors, a dimer binding site with DNA forms a leucine zipper.
This motif includes two amphipathic helices, one from each subunit, interacting with each other resulting in a left-handed coiled-coil super secondary structure. A leucine zipper is an interdigitation of regularly spaced leucine residues in one helix with leucines from an adjacent helix. Mostly, helices involved in leucine zippers exhibit a heptad sequence (abcdefg) with residues a and d being hydrophobic and other residues being hydrophilic.
Leucine zipper motifs can mediate either homo- or heterodimer formation.
In some embodiments, a DNA-binding domain comprises a Zn finger domain, where a Zn++ ion is coordinated by 2 Cys and 2 His residues. Such a transcription factor includes a trimer with the stoichiometry 1313 'a. An apparent effect of Zn++ coordination is stabilization of a small complex structure instead of hydrophobic core residues. Each Zn-finger interacts in a conformationally identical manner with successive triple base pair segments in the major groove of the double helix. Protein-DNA
interaction is determined by two factors: (i) H-bonding interaction between a-helix and DNA segment, mostly between Arg residues and Guanine bases. (ii) H-bonding interaction with DNA phosphate backbone, mostly with Arg and His. An alternative Zn-finger motif chelates Zn++ with 6 Cys.
An exemplary repressor domain may include, but is not limited to: ubiquitin, bicyclic peptides as ubiquitin ligase inhibitors, transcription factors, DNA and protein modification enzymes such as topoisomerases, topoisomerase inhibitors such as topotecan, DNA
methyltransferases such as the DNMT
family (e.g., DNMT3A, DNMT3B, DNMT3L), protein methyltransferases (e.g., viral lysine methyltransferase (vSET), protein-lysine N-methyltransferase (SMYD2), deaminases (e.g., APOBEC, UG1), histone methyltransferases such as enhancer of zeste homolog 2 (EZH2), PRMT1, histone-lysine-N-methyltransferase (Setdbl), histone methyltransferase (SET2), euchromatic histone-lysine N-methyltransferase 2 (G9A), histone-lysine N-methyltransferase (SUV39H1), and G9a), histone deacetylase (e.g., HDAC1, HDAC2, HDAC3, HDAC8), enzymes with a role in DNA
demethylation (e.g., the TET family enzymes catalyze oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and higher oxidative derivatives), protein demethylases such as KDM1A and lysine-specific histone demethylase 1 (LSD1), transcription repressors (e.g., KRAB, FOG1), helicases such as DHX9, deacetylases (e.g., sirtuin 1, 2, 3, 4, 5, 6, or 7), kinases, phosphatases, DNA-intercalating agents such as ethidium bromide, SYBR
green, and proflavine, efflux pump inhibitors such as peptidomimetics like phenylalanine arginyl 13-naphthylamide or quinoline derivatives, nuclear receptor activators and inhibitors, proteasome inhibitors, competitive inhibitors for enzymes such as those involved in lysosomal storage diseases, protein synthesis inhibitors, nucleases (e.g., Cpfl, Cas9, zinc finger nuclease), fusions of one or more thereof (e.g., dCas9-DNMT, dCas9-APOBEC, dCas9-UG1), and specific domains from proteins, such as a KRAB domain.
In some embodiments, a candidate domain may be determined to be suitable for use as a repressor domain by methods known to those of skill in the art. For example, a candidate repressor domain may be tested by assaying whether, when the candidate repressor domain is present in the nucleus of a cell and appropriately localized (e.g., to a target gene or transcription control element operably linked to said target gene, e.g., via a DNA-targeting moiety), the candidate repressor domain decreases expression of the target gene in the cell, e.g., decreases the level of RNA
transcript encoded by the target gene (e.g., as measured by RNASeq or Northern blot) or decreases the level of protein encoded by the target gene (e.g., as measured by ELISA).
In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each member of the plurality of expression repressors comprises a repressor domain, wherein each repressor domain does not detectably bind, e.g., does not bind, to another repressor domain.
In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor domain, wherein the first repressor domain does not detectably bind, e.g., does not bind, to the second repressor domain. In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor domain, wherein the first repressor domain does not detectably bind, e.g., does not bind, to another first repressor domain, and the second repressor domain does not detectably bind, e.g., does not bind, to another second repressor domain. In some embodiments, a repressor domain for use in the compositions and methods described herein is functional in a monomeric, e.g., non-dimeric, state.
In some embodiments, a repressor domain is or comprises an epigenetic modifying moiety, e.g., that modulates the two-dimensional structure of chromatin (i.e., that modulate structure of chromatin in a way that would alter its two-dimensional representation).
Epigenetic modifying moieties useful in methods and compositions of the present disclosure include agents that affect epigenetic markers, e.g., DNA methylation, histone methylation, histone acetylation, histone sumoylation, histone phosphorylation, and RNA-associated silencing. Exemplary epigenetic enzymes that can be targeted to a genomic sequence element as described herein include DNA
methylases (e.g., DNMT3a, DNMT3b, DNMTL), DNA demethylation (e.g., the TET
family), histone methyltransferases, histone deacetylase (e.g., HDAC1, HDAC2, HDAC3), sirtuin 1, 2, 3, 4, 5, 6, or 7, lysine-specific histone demethylase 1 (LSD1), histone-lysine-N-methyltransferase (Setdbl), euchromatic histone-lysine N-methyltransferase 2 (G9a), histone-lysine N-methyltransferase (SUV39H1), enhancer of zeste homolog 2 (EZH2), viral lysine methyltransferase (vSET), histone methyltransferase (SET2), and protein-lysine N-methyltransferase (SMYD2). Examples of such epigenetic modifying agents are described, e.g., in de Groote et al. Nuc. Acids Res. (2012):1-18.
In some embodiments, an expression repressor, e.g., comprising an epigenetic modifying moiety, useful herein comprises or is a construct described in Koferle et al. Genome Medicine 7.59 (2015):1-3incorporated herein by reference. For example, in some embodiments, an expression repressor comprises or is a construct found in Table 1 of Koferle et al., e.g., histone deacetylase, histone methyltransferase, DNA demethylation, or H3K4 and/or H3K9 histone demethylase described in Table 1 (e.g., dCas9-p300, TALE-TET1, ZF-DNMT3A, or TALE-LSD1).
Additional Moieties An expression repressor may further comprise one or more additional moieties (e.g., in addition to one or more DNA-targeting moieties and one or more repressor domains). In some embodiments, an additional moiety is selected from a tagging or monitoring moiety, a cleavable moiety (e.g., a cleavable moiety positioned between a DNA-targeting moiety and a repressor domain or at the N- or C-terminal end of a polypeptide), a small molecule, a membrane translocating polypeptide, or a pharmacoagent moiety.
Exemplary Expression Repressors The following exemplary expression repressors are presented for illustration purposes only and are not intended to be limiting.
In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. aureus dCas9, and a repressor domain comprising MQ1, e.g., bacterial MQ1. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NO: 91 (e.g., a plasmid encoding the expression repressor), SEQ ID NO: 22 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor) and/or SEQ ID NO: 92 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 22, 91 or 92 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto. In some embodiments, the DNA-targeting moiety is encoded by the nucleic acid sequence of SEQ ID NO: 45 or 89 and/or the repressor domain is encoded by the nucleic acid sequence of SEQ ID NO: 47.
dCas9-MQ1 (MR-28125) mRNA sequence:
AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAAGCGGCCCGCCG
CCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGCCCGGGACAGCAAGGTGGAGAACA
AGACCAAGAAGCTGCGGGTGTTCGAGGCCTTCGCCGGCATCGGCGCCCAGCGGAAGGCCCT
GGAGAAGGTGCGGAAGGACGAGTACGAGATCGTGGGCCTGGCCGAGTGGTACGTGCCCGCC
ATCGTGATGTACCAGGCCATCCACAACAACTTCCACACCAAGCTGGAGTACAAGAGCGTGA
GCCGGGAGGAGATGATCGACTACCTGGAGAACAAGACCCTGAGCTGGAACAGCAAGAACCC
CGTGAGCAACGGCTACTGGAAGCGGAAGAAGGACGACGAGCTGAAGATCATCTACAACGCC
ATCAAGCTGAGCGAGAAGGAGGGCAACATCTTCGACATCCGGGACCTGTACAAGCGGACCC
TGAAGAACATCGACCTGCTGACCTACAGCTTCCCCTGCCAGGACCTGAGCCAGCAGGGCATC
CAGAAGGGCATGAAGCGGGGCAGCGGCACCCGGAGCGGCCTGCTGTGGGAGATCGAGCGG
GCCCTGGACAGCACCGAGAAGAACGACCTGCCCAAGTACCTGCTGATGGAGAACGTGGGCG
CCCTGCTGCACAAGAAGAACGAGGAGGAGCTGAACCAGTGGAAGCAGAAGCTGGAGAGCC
TGGGCTACCAGAACAGCATCGAGGTGCTGAACGCCGCCGACTTCGGCAGCAGCCAGGCCCG
GCGGCGGGTGTTCATGATCAGCACCCTGAACGAGTTCGTGGAGCTGCCCAAGGGCGACAAG
AAGCCCAAGAGCATCAAGAAGGTGCTGAACAAGATCGTGAGCGAGAAGGACATCCTGAAC
AACCTGCTGAAGTACAACCTGACCGAGTTCAAGAAAACCAAGAGCAACATCAACAAGGCCA
GCCTGATCGGCTACAGCAAGTTCAACAGCGAGGGCTACGTGTACGACCCCGAGTTCACCGG
CCCCACCCTGACCGCCAGCGGCGCCAACAGCCGGATCAAGATCAAGGACGGCAGCAACATC
CGGAAGATGAACAGCGACGAGACCTTCCTGTACATCGGCTTCGACAGCCAGGACGGCAAGC
GGGTGAACGAGATCGAGTTCCTGACCGAGAACCAGAAGATCTTCGTGTGCGGCAACAGCAT
CAGCGTGGAGGTGCTGGAGGCCATCATCGACAAGATCGGCGGCCCCAGCAGCGGCGGCAAG
CGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTAC
GACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTC
TGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTA
GGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 22) Sa-dCas9-MQ1 (PL-27695) Plasmid DNA sequence:
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACA
GCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTG
GCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCAT
ATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGC
CATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAG
CTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGT
CACGACGTTGTAAAACGACGGCCAGTGAATTGACGCGTATTGGGATGGTACCTAATACGACT
CACTATAAGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCC
CCCAAGAAGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGCCAAGCGGAACTACA
TCCTGGGCCTGGCCATCGGCATCACCAGCGTGGGCTACGGCATCATCGACTACGAGACCCGG
GACGTGATCGACGCCGGCGTGCGGCTGTTCAAGGAGGCCAACGTGGAGAACAACGAGGGCC
GGCGGAGCAAGCGGGGCGCCCGGCGGCTGAAGCGGCGGCGGCGGCACCGGATCCAGCGGG
TGAAGAAGCTGCTGTTCGACTACAACCTGCTGACCGACCACAGCGAGCTGAGCGGCATCAA
CCCCTACGAGGCCCGGGTGAAGGGCCTGAGCCAGAAGCTGAGCGAGGAGGAGTTCAGCGCC
GCCCTGCTGCACCTGGCCAAGCGGCGGGGCGTGCACAACGTGAACGAGGTGGAGGAGGACA
CCGGCAACGAGCTGAGCACCAAGGAGCAGATCAGCCGGAACAGCAAGGCCCTGGAGGAGA
AGTACGTGGCCGAGCTGCAGCTGGAGCGGCTGAAGAAGGACGGCGAGGTGCGGGGCAGCA
TCAACCGGTTCAAGACCAGCGACTACGTGAAGGAGGCCAAGCAGCTGCTGAAGGTGCAGAA
GGCCTACCACCAGCTGGACCAGAGCTTCATCGACACCTACATCGACCTGCTGGAGACCCGGC
GGACCTACTACGAGGGCCCCGGCGAGGGCAGCCCCTTCGGCTGGAAGGACATCAAGGAGTG
GTACGAGATGCTGATGGGCCACTGCACCTACTTCCCCGAGGAGCTGCGGAGCGTGAAGTAC
GCCTACAACGCCGACCTGTACAACGCCCTGAACGACCTGAACAACCTGGTGATCACCCGGG
ACGAGAACGAGAAGCTGGAGTACTACGAGAAGTTCCAGATCATCGAGAACGTGTTCAAGCA
GAAGAAGAAGCCCACCCTGAAGCAGATCGCCAAGGAGATCCTGGTGAACGAGGAGGACAT
CAAGGGCTACCGGGTGACCAGCACCGGCAAGCCCGAGTTCACCAACCTGAAGGTGTACCAC
GACATCAAGGACATCACCGCCCGGAAGGAGATCATCGAGAACGCCGAGCTGCTGGACCAGA
TCGCCAAGATCCTGACCATCTACCAGAGCAGCGAGGACATCCAGGAGGAGCTGACCAACCT
GAACAGCGAGCTGACCCAGGAGGAGATCGAGCAGATCAGCAACCTGAAGGGCTACACCGG
CACCCACAACCTGAGCCTGAAGGCCATCAACCTGATCCTGGACGAGCTGTGGCACACCAAC
GACAACCAGATCGCCATCTTCAACCGGCTGAAGCTGGTGCCCAAGAAGGTGGACCTGAGCC
AGCAGAAGGAGATCCCCACCACCCTGGTGGACGACTTCATCCTGAGCCCCGTGGTGAAGCG
GAGCTTCATCCAGAGCATCAAGGTGATCAACGCCATCATCAAGAAGTACGGCCTGCCCAAC
GACATCATCATCGAGCTGGCCCGGGAGAAGAACAGCAAGGACGCCCAGAAGATGATCAACG
AGATGCAGAAGCGGAACCGGCAGACCAACGAGCGGATCGAGGAGATCATCCGGACCACCG
GCAAGGAGAACGCCAAGTACCTGATCGAGAAGATCAAGCTGCACGACATGCAGGAGGGCA
AGTGCCTGTACAGCCTGGAGGCCATCCCCCTGGAGGACCTGCTGAACAACCCCTTCAACTAC
GAGGTGGACGCCATCATCCCCCGGAGCGTGAGCTTCGACAACAGCTTCAACAACAAGGTGC
TGGTGAAGCAGGAGGAGAACAGCAAGAAGGGCAACCGGACCCCCTTCCAGTACCTGAGCAG
CAGCGACAGCAAGATCAGCTACGAGACCTTCAAGAAGCACATCCTGAACCTGGCCAAGGGC
AAGGGCCGGATCAGCAAGACCAAGAAGGAGTACCTGCTGGAGGAGCGGGACATCAACCGG
TTCAGCGTGCAGAAGGACTTCATCAACCGGAACCTGGTGGACACCCGGTACGCCACCCGGG
GCCTGATGAACCTGCTGCGGAGCTACTTCCGGGTGAACAACCTGGACGTGAAGGTGaaatccAT
CAACGGCGGCTTCACCAGCTTCCTGCGGCGGAAGTGGAAGTTCAAGAAGGAGCGGAACAAG
GGCTACAAGCACCACGCCGAGGACGCCCTGATCATCGCCAACGCCGACTTCATCTTCAAGGA
GTGGAAGAAGCTGGACAAGGCCAAGAAGGTGATGGAGAACCAGATGTTCGAGGAGAAGCA
GGCCGAGAGCATGCCCGAGATCGAGACCGAGCAGGAGTACAAGGAGATCTTCATCACCCCC
CACCAGATCAAGCACATCAAGGACTTCAAGGACTACAAGTACAGCCACCGGGTGGACAAGA
AGCCCAACCGGAAGCTGATCAACGACACCCTGTACAGCACCCGGAAGGACGACAAGGGCAA
CACCCTGATCGTGAACAACCTGAACGGCCTGTACGACAAGGACAACGACAAGCTGAAGAAG
CTGATCAACAAGAGCCCCGAGAAGCTGCTGATGTACCACCACGACCCCCAGACCTACCAGA
AGCTGAAGCTGATCATGGAGCAGTACGGCGACGAGAAGAACCCCCTGTACAAGTACTACGA
GGAGACCGGCAACTACCTGACCAAGTACAGCAAGAAGGACAACGGCCCCGTGATCAAGAA
GATCAAGTACTACGGCAACAAGCTGAACGCCCACCTGGACATCACCGACGACTACCCCAAC
AGCCGGAACAAGGTGGTGAAGCTGAGCCTGAAGCCCTACCGGTTCGACGTGTACCTGGACA
ACGGCGTGTACAAGTTCGTGACCGTGAAGAACCTGGACGTGATCAAGAAGGAGAACTACTA
CGAGGTGAACAGCAAGTGCTACGAGGAGGCCAAGAAGCTGAAGAAGATCAGCAACCAGGC
CGAGTTCATCGCCAGCTTCTACAAGAACGACCTGATCAAGATCAACGGCGAGCTGTACCGG
GTGATCGGCGTGAACAACGACCTGCTGAACCGGATCGAGGTGAACATGATCGACATCACCT
ACCGGGAGTACCTGGAGAACATGAACGACAAGCGGCCCCCCCACATCATCAAGACCATCGC
CAGCAAGACCCAGAGCATCAAGAAGTACAGCACCGACATCCTGGGCAACCTGTACGAGGTG
aaatccAAGAAGCACCCCCAGATCATCAAGAAGGGCAAGCGGCCCGCCGCCACCAAGAAGGCC
GGCCAGGCCAAGAAGAAGAAGGCCCGGGACAGCAAGGTGGAGAACAAGACCAAGAAGCTG
CGGGTGTTCGAGGCCTTCGCCGGCATCGGCGCCCAGCGGAAGGCCCTGGAGAAGGTGCGGA
AGGACGAGTACGAGATCGTGGGCCTGGCCGAGTGGTACGTGCCCGCCATCGTGATGTACCA
GGCCATCCACAACAACTTCCACACCAAGCTGGAGTACAAGAGCGTGAGCCGGGAGGAGATG
ATCGACTACCTGGAGAACAAGACCCTGAGCTGGAACAGCAAGAACCCCGTGAGCAACGGCT
ACTGGAAGCGGAAGAAGGACGACGAGCTGAAGATCATCTACAACGCCATCAAGCTGAGCGA
GAAGGAGGGCAACATCTTCGACATCCGGGACCTGTACAAGCGGACCCTGAAGAACATCGAC
CTGCTGACCTACAGCTTCCCCTGCCAGGACCTGAGCCAGCAGGGCATCCAGAAGGGCATGA
AGCGGGGCAGCGGCACCCGGAGCGGCCTGCTGTGGGAGATCGAGCGGGCCCTGGACAGCAC
CGAGAAGAACGACCTGCCCAAGTACCTGCTGATGGAGAACGTGGGCGCCCTGCTGCACAAG
AAGAACGAGGAGGAGCTGAACCAGTGGAAGCAGAAGCTGGAGAGCCTGGGCTACCAGAAC
AGCATCGAGGTGCTGAACGCCGCCGACTTCGGCAGCAGCCAGGCCCGGCGGCGGGTGTTCA
TGATCAGCACCCTGAACGAGTTCGTGGAGCTGCCCAAGGGCGACAAGAAGCCCAAGAGCAT
CAAGAAGGTGCTGAACAAGATCGTGAGCGAGAAGGACATCCTGAACAACCTGCTGAAGTAC
AACCTGACCGAGTTCAAGAAaACCAAGAGCAACATCAACAAGGCCAGCCTGATCGGCTACA
GCAAGTTCAACAGCGAGGGCTACGTGTACGACCCCGAGTTCACCGGCCCCACCCTGACCGCC
AGCGGCGCCAACAGCCGGATCAAGATCAAGGACGGCAGCAACATCCGGAAGATGAACAGC
GACGAGACCTTCCTGTACATCGGCTTCGACAGCCAGGACGGCAAGCGGGTGAACGAGATCG
AGTTCCTGACCGAGAACCAGAAGATCTTCGTGTGCGGCAACAGCATCAGCGTGGAGGTGCT
GGAGGCCATCATCGACAAGATCGGCGGCCCCAGCAGCGGCGGCAAGCGGCCCGCCGCCACC
AAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTAC
GCCTGAGcggccgcttaattaagctgccttctgcggggcttgccttctggccatgcccttcttctctcccttgcacctg tacctcttggtctttgaataaa gcctgagtaggaagtctagaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa aaaaaaaaaaaaaaaaa aaattgtcttcttcatcgcctgcagATCCCAATGGCGCGCCGAGCTTGGCTCGAGCATGGTCATAGCTGTTTC
CTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT
AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC
TTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA
GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTT
CGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGA
CGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTA
TCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC
CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGG
TGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGT
TACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTG
ATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCAT
GAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAA
TCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTAGAAAAACTCATCGAGCATCAAATGA
AACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT
GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGA
TTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAA
GTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCT
TTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAA
ACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGAC
AATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATT
TTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCAGGGATCGCAGTGGT
GAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAAT
TCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCA
TGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGA
TTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAA
TCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTA
TTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAA
ATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAAC
CATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC (SEQ ID
NO: 91) Sa-dCas9-MQ1 (MR-28126) Expressed mRNA sequence:
AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGCCAAGCGGAACTACATCCTGGG
CCTGGCCATCGGCATCACCAGCGTGGGCTACGGCATCATCGACTACGAGACCCGGGACGTG
ATCGACGCCGGCGTGCGGCTGTTCAAGGAGGCCAACGTGGAGAACAACGAGGGCCGGCGGA
GCAAGCGGGGCGCCCGGCGGCTGAAGCGGCGGCGGCGGCACCGGATCCAGCGGGTGAAGA
AGCTGCTGTTCGACTACAACCTGCTGACCGACCACAGCGAGCTGAGCGGCATCAACCCCTAC
GAGGCCCGGGTGAAGGGCCTGAGCCAGAAGCTGAGCGAGGAGGAGTTCAGCGCCGCCCTGC
TGCACCTGGCCAAGCGGCGGGGCGTGCACAACGTGAACGAGGTGGAGGAGGACACCGGCA
ACGAGCTGAGCACCAAGGAGCAGATCAGCCGGAACAGCAAGGCCCTGGAGGAGAAGTACG
TGGCCGAGCTGCAGCTGGAGCGGCTGAAGAAGGACGGCGAGGTGCGGGGCAGCATCAACC
GGTTCAAGACCAGCGACTACGTGAAGGAGGCCAAGCAGCTGCTGAAGGTGCAGAAGGCCTA
CCACCAGCTGGACCAGAGCTTCATCGACACCTACATCGACCTGCTGGAGACCCGGCGGACCT
ACTACGAGGGCCCCGGCGAGGGCAGCCCCTTCGGCTGGAAGGACATCAAGGAGTGGTACGA
GATGCTGATGGGCCACTGCACCTACTTCCCCGAGGAGCTGCGGAGCGTGAAGTACGCCTACA
ACGCCGACCTGTACAACGCCCTGAACGACCTGAACAACCTGGTGATCACCCGGGACGAGAA
CGAGAAGCTGGAGTACTACGAGAAGTTCCAGATCATCGAGAACGTGTTCAAGCAGAAGAAG
AAGCCCACCCTGAAGCAGATCGCCAAGGAGATCCTGGTGAACGAGGAGGACATCAAGGGCT
ACCGGGTGACCAGCACCGGCAAGCCCGAGTTCACCAACCTGAAGGTGTACCACGACATCAA
GGACATCACCGCCCGGAAGGAGATCATCGAGAACGCCGAGCTGCTGGACCAGATCGCCAAG
ATCCTGACCATCTACCAGAGCAGCGAGGACATCCAGGAGGAGCTGACCAACCTGAACAGCG
AGCTGACCCAGGAGGAGATCGAGCAGATCAGCAACCTGAAGGGCTACACCGGCACCCACAA
CCTGAGCCTGAAGGCCATCAACCTGATCCTGGACGAGCTGTGGCACACCAACGACAACCAG
ATCGCCATCTTCAACCGGCTGAAGCTGGTGCCCAAGAAGGTGGACCTGAGCCAGCAGAAGG
AGATCCCCACCACCCTGGTGGACGACTTCATCCTGAGCCCCGTGGTGAAGCGGAGCTTCATC
CAGAGCATCAAGGTGATCAACGCCATCATCAAGAAGTACGGCCTGCCCAACGACATCATCA
TCGAGCTGGCCCGGGAGAAGAACAGCAAGGACGCCCAGAAGATGATCAACGAGATGCAGA
AGCGGAACCGGCAGACCAACGAGCGGATCGAGGAGATCATCCGGACCACCGGCAAGGAGA
ACGCCAAGTACCTGATCGAGAAGATCAAGCTGCACGACATGCAGGAGGGCAAGTGCCTGTA
CAGCCTGGAGGCCATCCCCCTGGAGGACCTGCTGAACAACCCCTTCAACTACGAGGTGGAC
GCCATCATCCCCCGGAGCGTGAGCTTCGACAACAGCTTCAACAACAAGGTGCTGGTGAAGC
AGGAGGAGAACAGCAAGAAGGGCAACCGGACCCCCTTCCAGTACCTGAGCAGCAGCGACA
GCAAGATCAGCTACGAGACCTTCAAGAAGCACATCCTGAACCTGGCCAAGGGCAAGGGCCG
GATCAGCAAGACCAAGAAGGAGTACCTGCTGGAGGAGCGGGACATCAACCGGTTCAGCGTG
CAGAAGGACTTCATCAACCGGAACCTGGTGGACACCCGGTACGCCACCCGGGGCCTGATGA
ACCTGCTGCGGAGCTACTTCCGGGTGAACAACCTGGACGTGAAGGTGaaatccATCAACGGCGG
CTTCACCAGCTTCCTGCGGCGGAAGTGGAAGTTCAAGAAGGAGCGGAACAAGGGCTACAAG
CACCACGCCGAGGACGCCCTGATCATCGCCAACGCCGACTTCATCTTCAAGGAGTGGAAGA
AGCTGGACAAGGCCAAGAAGGTGATGGAGAACCAGATGTTCGAGGAGAAGCAGGCCGAGA
GCATGCCCGAGATCGAGACCGAGCAGGAGTACAAGGAGATCTTCATCACCCCCCACCAGAT
CAAGCACATCAAGGACTTCAAGGACTACAAGTACAGCCACCGGGTGGACAAGAAGCCCAAC
CGGAAGCTGATCAACGACACCCTGTACAGCACCCGGAAGGACGACAAGGGCAACACCCTGA
TCGTGAACAACCTGAACGGCCTGTACGACAAGGACAACGACAAGCTGAAGAAGCTGATCAA
CAAGAGCCCCGAGAAGCTGCTGATGTACCACCACGACCCCCAGACCTACCAGAAGCTGAAG
CTGATCATGGAGCAGTACGGCGACGAGAAGAACCCCCTGTACAAGTACTACGAGGAGACCG
GCAACTACCTGACCAAGTACAGCAAGAAGGACAACGGCCCCGTGATCAAGAAGATCAAGTA
CTACGGCAACAAGCTGAACGCCCACCTGGACATCACCGACGACTACCCCAACAGCCGGAAC
AAGGTGGTGAAGCTGAGCCTGAAGCCCTACCGGTTCGACGTGTACCTGGACAACGGCGTGT
ACAAGTTCGTGACCGTGAAGAACCTGGACGTGATCAAGAAGGAGAACTACTACGAGGTGAA
CAGCAAGTGCTACGAGGAGGCCAAGAAGCTGAAGAAGATCAGCAACCAGGCCGAGTTCATC
GCCAGCTTCTACAAGAACGACCTGATCAAGATCAACGGCGAGCTGTACCGGGTGATCGGCG
TGAACAACGACCTGCTGAACCGGATCGAGGTGAACATGATCGACATCACCTACCGGGAGTA
CCTGGAGAACATGAACGACAAGCGGCCCCCCCACATCATCAAGACCATCGCCAGCAAGACC
CAGAGCATCAAGAAGTACAGCACCGACATCCTGGGCAACCTGTACGAGGTGaaatccAAGAAG
CACCCCCAGATCATCAAGAAGGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCA
AGAAGAAGAAGGCCCGGGACAGCAAGGTGGAGAACAAGACCAAGAAGCTGCGGGTGTTCG
AGGCCTTCGCCGGCATCGGCGCCCAGCGGAAGGCCCTGGAGAAGGTGCGGAAGGACGAGTA
CGAGATCGTGGGCCTGGCCGAGTGGTACGTGCCCGCCATCGTGATGTACCAGGCCATCCACA
ACAACTTCCACACCAAGCTGGAGTACAAGAGCGTGAGCCGGGAGGAGATGATCGACTACCT
GGAGAACAAGACCCTGAGCTGGAACAGCAAGAACCCCGTGAGCAACGGCTACTGGAAGCG
GAAGAAGGACGACGAGCTGAAGATCATCTACAACGCCATCAAGCTGAGCGAGAAGGAGGG
CAACATCTTCGACATCCGGGACCTGTACAAGCGGACCCTGAAGAACATCGACCTGCTGACCT
ACAGCTTCCCCTGCCAGGACCTGAGCCAGCAGGGCATCCAGAAGGGCATGAAGCGGGGCAG
CGGCACCCGGAGCGGCCTGCTGTGGGAGATCGAGCGGGCCCTGGACAGCACCGAGAAGAAC
GACCTGCCCAAGTACCTGCTGATGGAGAACGTGGGCGCCCTGCTGCACAAGAAGAACGAGG
AGGAGCTGAACCAGTGGAAGCAGAAGCTGGAGAGCCTGGGCTACCAGAACAGCATCGAGG
TGCTGAACGCCGCCGACTTCGGCAGCAGCCAGGCCCGGCGGCGGGTGTTCATGATCAGCAC
CCTGAACGAGTTCGTGGAGCTGCCCAAGGGCGACAAGAAGCCCAAGAGCATCAAGAAGGTG
CTGAACAAGATCGTGAGCGAGAAGGACATCCTGAACAACCTGCTGAAGTACAACCTGACCG
AGTTCAAGAAaACCAAGAGCAACATCAACAAGGCCAGCCTGATCGGCTACAGCAAGTTCAA
CAGCGAGGGCTACGTGTACGACCCCGAGTTCACCGGCCCCACCCTGACCGCCAGCGGCGCC
AACAGCCGGATCAAGATCAAGGACGGCAGCAACATCCGGAAGATGAACAGCGACGAGACC
TTCCTGTACATCGGCTTCGACAGCCAGGACGGCAAGCGGGTGAACGAGATCGAGTTCCTGAC
CGAGAACCAGAAGATCTTCGTGTGCGGCAACAGCATCAGCGTGGAGGTGCTGGAGGCCATC
ATCGACAAGATCGGCGGCCCCAGCAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCG
GCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGcggccg cttaattaagctgccttctgcggggcttgccttctggccatgcccttcttctctcccttgcacctgtacctcttggtct ttgaataaagcctgagtaggaagtcta gaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa aa (SEQ ID NO:
92) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 93, 33, 67, or 68. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 93, 33, 67, 68, or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
Sa-dCas9-MQ1 Protein sequence:
MAPKKKRKVGIHGVPAAAKRNYILGLAIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRR
SKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLA
KRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYV
KEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYF
PEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKEILV
NEEDIKGYRVT STGKPEFTNLKVYHDIKDITARKEIIENAELLD QIAKILTIY QS S EDIQEELTNLNS
ELT QEEIEQIS NLKGYTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLS QQKEIPTTL
VDDFILSPVVKRSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEII
RTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDAIIPRSVSFDNSFNNKVLV
.. KQEENS KKGNRTPFQYLSS SD S KISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFI
NRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKS INGGFT SFLRRKWKFKKERNKGYKHHAED
ALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQIKHIKDFKDYK
YSHRVDKKPNRKLINDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLKKLINKSPEKLLMYHHD
PQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYS KKDNGPVIKKIKYYGNKLNAHLDITDD
YPNS RNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNS KCYEEAKKLKKIS NQ
AEFIAS FYKNDLIKINGELYRVIGVNNDLLNRIEVNMIDITYREYLENMND KRPPHIIKTIAS KT QSI
KKYSTDILGNLYEVKSKKHPQIIKKGKRPAATKKAGQAKKKKARDSKVENKTKKLRVFEAFAGI
GAQRKALEKVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEYKSVSREEMIDYLENKTLSW
NS KNPV SNGYWKRKKDDELKIIYNAIKLS EKEGNIFDIRDLYKRTLKNIDLLTYSFPC QDLS QQGI
QKGMKRGSGTRSGLLWEIERALDSTEKNDLPKYLLMENVGALLHKKNEEELNQWKQKLESLG
YQNSIEVLNAADFGS S QARRRVFMISTLNEFVELPKGDKKPKSIKKVLNKIVSEKDILNNLLKYNL
TEFKKTKSNINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRIKIKDGSNIRKMNSDETFLYIG
FDS QDGKRVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGGPSSGGKRPAATKKAGQAKKKKGSY
PYDVPDYA (SEQ ID NO: 93) dCas9-MQ1 amino acid sequence MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGD KRPAATKKAGQAKKKKARD S KVENKTKKLRVFEAFAGIGAQRKALE
KVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEYKSV SREEMIDYLENKTLSWNS KNPVS N
GYWKRKKDDELKIIYNAIKLSEKEGNIFDIRDLYKRTLKNIDLLTYSFPCQDLSQQGIQKGMKRG
SGTRSGLLWEIERALDSTEKNDLPKYLLMENVGALLHKKNEEELNQWKQKLESLGYQNSIEVLN
AADFGS SQARRRVFMISTLNEFVELPKGDKKPKSIKKVLNKIVSEKDILNNLLKYNLTEFKKTKS
NINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRIKIKDGSNIRKMNSDETFLYIGFDS QDGK
RVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGGPSSGGKRPAATKKAGQAKKKKGSYPYDVPDY
A (SEQ ID NO: 33) Sa-dCas9-MQ1 without HA tag MAPKKKRKVGIHGVPAAAKRNYILGLAIGITS VGYGIIDYETRDVIDAGVRLFKEANVENNEGRR
S KRGARRLKRRRRHRIQRV KKLLFDYNLLTDHSELS GINPYEARVKGLS QKLSEEEFSAALLHLA
KRRGVHNVNEVEEDTGNELSTKEQISRNS KALEEKYVAELQLERLKKDGEVRGSINRFKTSDYV
KEAKQLLKV QKAYHQLD QSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYF
PEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKEILV
NEEDIKGYRVT STGKPEFTNLKVYHDIKDITARKEIIENAELLD QIAKILTIY QS S EDIQEELTNLNS
ELT QEEIEQIS NLKGYTGTHNLSLKAINLILDELWHTNDNQIAIFNRLKLVPKKVDLS QQKEIPTTL
VDDFILSPVVKRSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEII
RTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDAIIPRSVSFDNSFNNKVLV
KQEENS KKGNRTPFQYLSS SD S KISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFI
NRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKS INGGFT SFLRRKWKFKKERNKGYKHHAED
ALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQIKHIKDFKDYK
YSHRVDKKPNRKLINDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLKKLINKSPEKLLMYHHD
PQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYS KKDNGPVIKKIKYYGNKLNAHLDITDD
YPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAKKLKKISNQ
AEFIAS FYKNDLIKINGELYRVIGVNNDLLNRIEVNMIDITYREYLENMND KRPPHIIKTIAS KT QSI
KKYSTDILGNLYEVKSKKHPQIIKKGKRPAATKKAGQAKKKKARDSKVENKTKKLRVFEAFAGI
GAQRKALEKVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEYKSVSREEMIDYLENKTLSW
NS KNPV SNGYWKRKKDDELKIIYNAIKLS EKEGNIFDIRDLYKRTLKNIDLLTYSFPC QDLS QQGI
QKGMKRGSGTRSGLLWEIERALDS TEKNDLPKYLLMENVGALLHKKNEEELNQWKQKLESLG
YQNSIEVLNAADFGS S QARRRVFMISTLNEFVELPKGDKKPKSIKKVLNKIVSEKDILNNLLKYNL
TEFKKTKSNINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRIKIKDGSNIRKMNSDETFLYIG
FDS QDGKRVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGGPSSGGKRPAATKKAGQAKKKKGS
(SEQ ID NO: 67) .. dCas9-MQ1 without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGD KRPAATKKAGQAKKKKARD S KVENKTKKLRVFEAFAGIGAQRKALE
KVRKDEYEIVGLAEWYVPAIVMYQAIHNNFHTKLEYKSVSREEMIDYLENKTLSWNSKNPVSN
GYWKRKKDDELKIIYNAIKLSEKEGNIFDIRDLYKRTLKNIDLLTYSFPCQDLSQQGIQKGMKRG
SGTRSGLLWEIERALDSTEKNDLPKYLLMENVGALLHKKNEEELNQWKQKLESLGYQNSIEVLN
AADFGSSQARRRVFMISTLNEFVELPKGDKKPKSIKKVLNKIVSEKDILNNLLKYNLTEFKKTKS
NINKASLIGYSKFNSEGYVYDPEFTGPTLTASGANSRIKIKDGSNIRKMNSDETFLYIGFDSQDGK
RVNEIEFLTENQKIFVCGNSISVEVLEAIIDKIGGPSSGGKRPAATKKAGQAKKKKGS (SEQ ID
NO: 68) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising KRAB, e.g., a KRAB domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 94 (e.g., a plasmid encoding the expression repressor) and/or 95 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 94 or 95 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
Sp-dCas9-KRAB (PL-27687) Plasmid DNA sequence:
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACA
GCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTG
GCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCAT
ATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGC
CATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAG
CTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGT
CACGACGTTGTAAAACGACGGCCAGTGAATTGACGCGTATTGGGATGGTACCTAATACGACT
CACTATAAGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCC
CCCAAGAAGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGC
ATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGG
TGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCT
GATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACC
GCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCA
ACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGA
GGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTAC
CACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGG
CCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTG
ATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGC
AGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGC
CATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCC
GGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCA
ACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTA
CGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGG
CCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGAT
CACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTG
ACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCG
ACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTA
CAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTG
AACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGA
TCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAG
GACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCT
GGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGaaatccGAGGAGACCATCACCCCCTG
GAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACC
AACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGT
ACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCC
CGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGG
AAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCG
TGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCT
GAAGATCATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGA
CATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAaACCT
ACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTG
GGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATC
CTGGACTTCCTGaaatccGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACA
GCCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCA
CGAGCACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTG
AAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCG
AGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGA
AGCGGATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGG
AGAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACAT
GTACGTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTG
CCCCAGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGG
CCCGGGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACT
GGCGGCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGC
CGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAG
ACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACG
ACGAGAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGaaatccAAGCTGGTGAGCG
ACTTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCAC
GACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGA
GCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGA
GCAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCA
AGACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGG
CGAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTG
AGCATGCCCCAGGTGAACATCGTGAAGAAaACCGAGGTGCAGACCGGCGGCTTCAGCAAGG
AGAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCC
CAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAG
GTGGAGAAGGGCAAGAGCAAGAAGCTGaaatccGTGAAGGAGCTGCTGGGCATCACCATCATG
GAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGG
TGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCG
GAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGC
AAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGG
ACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGA
GCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCTG
AGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCACC
TGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGAC
CGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCA
CCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAAGCGGCCCGCCGC
CACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGCCAGCGACGCCAAGAGCCTGACCGC
CTGGAGCCGGACCCTGGTGACCTTCAAGGACGTGTTCGTGGACTTCACCCGGGAGGAGTGG
AAGCTGCTGGACACCGCCCAGCAGATCCTGTACCGGAACGTGATGCTGGAGAACTACAAGA
ACCTGGTGAGCCTGGGCTACCAGCTGACCAAGCCCGACGTGATCCTGCGGCTGGAGAAGGG
CGAGGAGCCCTGGCTGGTGGAGCGGGAGATCCACCAGGAGACCCACCCCGACAGCGAGACC
GCCTTCGAGATCAAGAGCAGCGTGAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCG
GCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGcggccg cttaattaagctgccttctgcggggcttgccttctggccatgcccttcttctctcccttgcacctgtacctcttggtct ttgaataaagcctgagtaggaagtcta gaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa aattgtcttcttcatcgcc tgcagATCCCAATGGCGCGCCGAGCTTGGCTCGAGCATGGTCATAGCTGTTTCCTGTGTGAAAT
TGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGG
GTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGG
GAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG
TATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCG
AGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCA
GGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTG
CTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTC
GTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGA
AGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC
AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTA
TCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA
CGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT
TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTAC
GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATA
TATGAGTAAACTTGGTCTGACAGTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTT
ATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAA
ACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGT
CCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATC
ACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTT
GTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTC
ATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAA
CAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGA
ATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCAGGGATCGCAGTGGTGAGTAACC
ATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAG
CCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAG
AAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGA
CATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGC
CTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTATTGAAGC
ATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACA
AATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTA
TCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC (SEQ ID NO: 94) Sp-dCas9-KRAB (MR-28122) Expressed mRNA sequence:
AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGaaatccGAGGAGACCATCACCCCCTGGAACTTCGA
GGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGAC
AAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCG
TGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTTCCT
GAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTGACC
GTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGATCA
GCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGATCAT
CAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTGCTG
ACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAaACCTACGCCCACCT
GTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTG
AGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCC
TGaaatccGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTT
CAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAGCACAT
CGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTG
GACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGATGGCCC
GGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCGGATCG
AGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAACACCC
AGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTACGTGGA
CCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCCAGAGC
TTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCGGGGCA
AGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCGGCAGC
TGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAGCGGGG
CGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCCGGCAG
ATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACG
ACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGaaatccAAGCTGGTGAGCGACTTCCGGA
AGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACGACGCCTA
CCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAGCGAGTTC
GTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAGA
TCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAG
ATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGCGAGACCG
GCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGAGCATGCC
CCAGGTGAACATCGTGAAGAAaACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGAGCATC
CTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGT
ACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGGTGGAGAA
GGGCAAGAGCAAGAAGCTGaaatccGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGAG
CAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAG
GACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGA
TGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGCAAGTACGT
GAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGGACAACGAG
CAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCA
GCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCTGAGCGCCTA
CAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACC
CTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAAGCG
GTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTG
TACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAAGCGGCCCGCCGCCACCAAGA
AGGCCGGCCAGGCCAAGAAGAAGAAGGCCAGCGACGCCAAGAGCCTGACCGCCTGGAGCC
GGACCCTGGTGACCTTCAAGGACGTGTTCGTGGACTTCACCCGGGAGGAGTGGAAGCTGCTG
GACACCGCCCAGCAGATCCTGTACCGGAACGTGATGCTGGAGAACTACAAGAACCTGGTGA
GCCTGGGCTACCAGCTGACCAAGCCCGACGTGATCCTGCGGCTGGAGAAGGGCGAGGAGCC
CTGGCTGGTGGAGCGGGAGATCCACCAGGAGACCCACCCCGACAGCGAGACCGCCTTCGAG
ATCAAGAGCAGCGTGAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCC
AAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGcggccgcttaattaagctgc cttctgcggggcttgccttctggccatgcccttcttctctcccttgcacctgtacctcttggtctttgaataaagcctg agtaggaagtctagaaaaaaaaaaa aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa (SEQ ID
NO: 95) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 96 or 72. In some embodiments, an expression repressor described herein comprises a an amino acid sequence of SEQ ID NO: 72 or 96 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
Sp-dCas9-KRAB Protein sequence:
MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDKRPAATKKAGQAKKKKASDAKSLTAWSRTLVTFKDVFVDFTREEWK
LLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIK
SSVSGGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 96) MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLSQLGGDKRPAATKKAGQAKKKKASDAKSLTAWSRTLVTFKDVFVDFTREEWK
LLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIK
SSVSGGKRPAATKKAGQAKKKKGS (SEQ ID NO: 72) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising DNMT1, e.g., a DNMT1 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 23 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 23 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT1 (MR-29419) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCT
CAGTGGATCTGAGGACACTCGACGTGTTTAGCGGATGCGGCGGACTCTCCGAAGGCTTCCAC
CAAGCCGGAATTTCCGACACACTCTGGGCCATTGAGATGTGGGACCCCGCCGCTCAAGCCTT
CAGACTGAATAATCCCGGCTCCACCGTGTTCACCGAGGACTGCAACATTCTGCTGAAGCTGG
TGATGGCTGGCGAAACCACCAACTCTAGAGGCCAGAGGCTGCCCCAGAAGGGAGATGTGGA
AATGCTCTGTGGAGGCCCTCCTTGCCAAGGCTTCTCCGGCATGAACAGGTTCAACTCTAGAA
CATACAGCAAGTTCAAGAACTCTCTGGTCGTGAGCTTTCTGAGCTACTGCGACTACTATAGA
CCTAGGTTCTTTCTGCTGGAGAACGTGAGAAATTTCGTGTCCTTCAAGAGGAGCATGGTGCT
GAAGCTGACACTGAGGTGTCTGGTGAGGATGGGCTACCAGTGCACATTCGGAGTGCTGCAA
GCTGGCCAGTACGGCGTGGCCCAGACCAGAAGGAGGGCCATCATTCTGGCTGCTGCCCCCG
GCGAGAAACTCCCTCTGTTCCCCGAGCCCCTCCACGTGTTCGCCCCTAGAGCTTGCCAGCTG
AGCGTGGTGGTCGACGATAAGAAGTTCGTGAGCAACATCACAAGGCTGTCCAGCGGACCCT
TCAGAACCATTACCGTGAGGGATACCATGTCCGACCTCCCCGAGGTGAGGAATGGCGCCAG
CGCTCTGGAGATTTCCTACAACGGCGAACCTCAGAGCTGGTTCCAAAGGCAGCTGAGAGGC
GCTCAGTATCAGCCCATTCTGAGGGACCACATCTGCAAAGATATGAGCGCTCTGGTGGCCGC
TAGAATGAGACATATTCCTCTGGCCCCCGGCAGCGACTGGAGAGATCTGCCCAATATTGAGG
TGAGACTCAGCGACGGAACAATGGCTAGAAAACTGAGGTACACCCATCATGATAGAAAGAA
CGGAAGGAGCAGCAGCGGCGCTCTGAGAGGAGTGTGTAGCTGCGTGGAAGCTGGCAAGGCT
TGCGATCCCGCCGCTAGGCAGTTCAATACCCTCATCCCTTGGTGTCTGCCTCACACCGGCAA
CAGACACAATCATTGGGCTGGACTGTATGGAAGGCTCGAATGGGACGGCTTTTTCAGCACCA
CCGTGACCAATCCCGAACCTATGGGCAAGCAAGGAAGGGTGCTCCACCCCGAGCAGCATAG
AGTCGTGTCCGTGAGAGAATGCGCTAGAAGCCAAGGCTTCCCCGACACCTATAGACTGTTCG
GCAACATTCTGGATAAGCACAGACAAGTGGGAAATGCTGTCCCTCCTCCTCTGGCCAAGGCT
ATCGGACTGGAGATCAAGCTGTGTATGCTCGCCAAAGCTAGGGAGAGCGCTTCCGCCAAGA
TTAAGGAGGAGGAGGCCGCCAAGGACGGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCA
CCAAGAAGGCCGGTCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTA
CGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCT
TCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAA (SEQ ID NO: 23) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 34 or 69. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 34 or 69, or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT
MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDIN
RLSDYDVAAIVPQSFLKDD SIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLIT Q
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSVDLRTLDVFSGCGGLSEGFHQ
AGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEML
CGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLR
CLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKK
FVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICK
DMSALVAARMRHIPLAPGSDWRDLPNIEVRLSD GTMARKLRYTHHDRKNGRS S SGALRGVC SC
VEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVL
HPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESAS
AKIKEEEAAKDGGGGSGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 34) dCas9-DNMT without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNL SDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSVDLRTLDVFSGCGGLSEGFHQ
AGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEML
CGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLR
CLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKK
FVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICK
DMSALVAARMRHIPLAPGSDWRDLPNIEVRLSD GTMARKLRYTHHDRKNGRS S SGALRGVC SC
VEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVL
HPEQHRVV SVRECARS QGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESAS
AKIKEEEAAKDGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID NO: 69) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising DNMT3a/3L. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 24 (e.g., a nucleic acid (e.g., cDNA) encoding a fully human expression repressor) and/or 25 (e.g., a nucleic acid (e.g., cDNA) encoding a chimeric expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 24 or 25 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT 3a/3L (h) (MR-29414) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGCCGGCGGCG
GCGGCAGCGGCGGCGGCGGCAGCGGCGGCGGCGGCAGCGGCCCCAAGAAGAAGCGGAAGG
TGGCCGCCGCCGGCAGCAACCACGACCAGGAGTTCGACCCCCCCAAGGTGTACCCCCCCGT
GCCCGCCGAGAAGCGGAAGCCCATCCGGGTGCTGAGCCTGTTCGACGGCATCGCCACCGGC
CTGCTGGTGCTGAAGGACCTGGGCATCCAGGTGGACCGGTACATCGCCAGCGAGGTGTGCG
AGGACAGCATCACCGTGGGCATGGTGCGGCACCAGGGCAAGATCATGTACGTGGGCGACGT
GCGGAGCGTGACCCAGAAGCACATCCAGGAGTGGGGCCCCTTCGACCTGGTGATCGGCGGC
AGCCCCTGCAACGACCTGAGCATCGTGAACCCCGCCCGGAAGGGCCTGTACGAGGGCACCG
GCCGGCTGTTCTTCGAGTTCTACCGGCTGCTGCACGACGCCCGGCCCAAGGAGGGCGACGAC
CGGCCCTTCTTCTGGCTGTTCGAGAACGTGGTGGCCATGGGCGTGAGCGACAAGCGGGACAT
CAGCCGGTTCCTGGAGAGCAACCCCGTGATGATCGACGCCAAGGAGGTGAGCGCCGCCCAC
CGGGCCCGGTACTTCTGGGGCAACCTGCCCGGCATGAACCGGCCCCTGGCCAGCACCGTGA
ACGACAAGCTGGAGCTGCAGGAGTGCCTGGAGCACGGCCGGATCGCCAAGTTCAGCAAGGT
GCGGACCATCACCACCCGGAGCAACAGCATCAAGCAGGGCAAGGACCAGCACTTCCCCGTG
TTCATGAACGAGAAGGAGGACATCCTGTGGTGCACCGAGATGGAGCGGGTGTTCGGCTTCC
CCGTGCACTACACCGACGTGAGCAACATGAGCCGGCTGGCCCGGCAGCGGCTGCTGGGCCG
GAGCTGGAGCGTGCCCGTGATCCGGCACCTGTTCGCCCCCCTGAAGGAGTACTTCGCCTGCG
TGAGCAGCGGCAACAGCAACGCCAACAGCCGGGGCCCCAGCTTCAGCAGCGGCCTGGTGCC
CCTGAGCCTGCGGGGCAGCCACATGAATCCTCTGGAGATGTTCGAGACAGTGCCCGTGTGGA
GAAGGCAACCCGTGAGGGTGCTGAGCCTCTTCGAGGACATTAAGAAGGAGCTGACCTCTCT
GGGCTTTCTGGAATCCGGCAGCGACCCCGGCCAGCTGAAACACGTGGTGGACGTGACCGAC
ACAGTGAGGAAGGACGTGGAAGAGTGGGGCCCCTTTGACCTCGTGTATGGAGCCACACCTC
CTCTCGGCCACACATGCGATAGGCCTCCCAGCTGGTATCTCTTCCAGTTCCACAGACTGCTCC
AGTACGCCAGACCTAAGCCCGGCAGCCCCAGACCCTTCTTCTGGATGTTCGTGGACAATCTG
GTGCTGAACAAGGAGGATCTGGATGTGGCCAGCAGATTTCTGGAGATGGAACCCGTGACAA
TCCCCGACGTGCATGGCGGCTCTCTGCAGAACGCCGTGAGAGTGTGGTCCAACATCCCCGCC
ATTAGAAGCAGACACTGGGCTCTGGTGAGCGAGGAGGAACTGTCTCTGCTGGCCCAGAATA
AGCAGTCCTCCAAGCTGGCCGCCAAGTGGCCCACCAAGCTGGTGAAGAACTGCTTTCTGCCT
CTGAGGGAGTATTTCAAGTATTTCAGCACCGAACTGACCAGCAGCCTGAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACG
ACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCT
GGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAG
GAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 24) dCas9-DNMT3a/3L (m) (MR-28195) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGCCGGCGGCG
GCGGCAGCGGCGGCGGCGGCAGCGGCGGCGGCGGCAGCGGCCCCAAGAAGAAGCGGAAGG
TGGCCGCCGCCGGCAGCAACCACGACCAGGAGTTCGACCCCCCCAAGGTGTACCCCCCCGT
GCCCGCCGAGAAGCGGAAGCCCATCCGGGTGCTGAGCCTGTTCGACGGCATCGCCACCGGC
CTGCTGGTGCTGAAGGACCTGGGCATCCAGGTGGACCGGTACATCGCCAGCGAGGTGTGCG
AGGACAGCATCACCGTGGGCATGGTGCGGCACCAGGGCAAGATCATGTACGTGGGCGACGT
GCGGAGCGTGACCCAGAAGCACATCCAGGAGTGGGGCCCCTTCGACCTGGTGATCGGCGGC
AGCCCCTGCAACGACCTGAGCATCGTGAACCCCGCCCGGAAGGGCCTGTACGAGGGCACCG
GCCGGCTGTTCTTCGAGTTCTACCGGCTGCTGCACGACGCCCGGCCCAAGGAGGGCGACGAC
CGGCCCTTCTTCTGGCTGTTCGAGAACGTGGTGGCCATGGGCGTGAGCGACAAGCGGGACAT
CAGCCGGTTCCTGGAGAGCAACCCCGTGATGATCGACGCCAAGGAGGTGAGCGCCGCCCAC
CGGGCCCGGTACTTCTGGGGCAACCTGCCCGGCATGAACCGGCCCCTGGCCAGCACCGTGA
ACGACAAGCTGGAGCTGCAGGAGTGCCTGGAGCACGGCCGGATCGCCAAGTTCAGCAAGGT
GCGGACCATCACCACCCGGAGCAACAGCATCAAGCAGGGCAAGGACCAGCACTTCCCCGTG
TTCATGAACGAGAAGGAGGACATCCTGTGGTGCACCGAGATGGAGCGGGTGTTCGGCTTCC
CCGTGCACTACACCGACGTGAGCAACATGAGCCGGCTGGCCCGGCAGCGGCTGCTGGGCCG
GAGCTGGAGCGTGCCCGTGATCCGGCACCTGTTCGCCCCCCTGAAGGAGTACTTCGCCTGCG
TGAGCAGCGGCAACAGCAACGCCAACAGCCGGGGCCCCAGCTTCAGCAGCGGCCTGGTGCC
CCTGAGCCTGCGGGGCAGCCACATGGGCCCCATGGAGATCTACAAGACCGTGAGCGCCTGG
AAGCGGCAGCCCGTGCGGGTGCTGAGCCTGTTCCGGAACATCGACAAGGTGCTGAAATCCC
TGGGCTTCCTGGAGAGCGGCAGCGGCAGCGGCGGCGGCACCCTGAAGTACGTGGAGGACGT
GACCAACGTGGTGCGGCGGGACGTGGAGAAGTGGGGCCCCTTCGACCTGGTGTACGGCAGC
ACCCAGCCCCTGGGCAGCAGCTGCGACCGGTGCCCCGGCTGGTACATGTTCCAGTTCCACCG
GATCCTGCAGTACGCCCTGCCCCGGCAGGAGAGCCAGCGGCCCTTCTTCTGGATCTTCATGG
ACAACCTGCTGCTGACCGAGGACGACCAGGAGACCACCACCCGGTTCCTGCAGACCGAGGC
CGTGACCCTGCAGGACGTGCGGGGCCGGGACTACCAGAACGCCATGCGGGTGTGGAGCAAC
ATCCCCGGCCTGAAATCCAAGCACGCCCCCCTGACCCCCAAGGAGGAGGAGTACCTGCAGG
CCCAGGTGCGGAGCCGGAGCAAGCTGGACGCCCCCAAGGTGGACCTGCTGGTGAAGAACTG
CCTGCTGCCCCTGCGGGAGTACTTCAAGTACTTCAGCCAGAACAGCCTGCCCCTGAGCGGCG
GCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACC
CCTACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTG
CCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTG
AGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 25) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 35, 36, 70, or 71. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 35, 36, 70, or 71 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT3a/3L (h) MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSNHDQEFDPPKVYPPVP
AEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQ
KHIQEWGPFDLVIGGSPCNDLS IVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFEN
VVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLE
HGRIAKFS KVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLAR
QRLLGRSWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMNPLEMFETVP
VWRRQPVRVLS LFEDIKKELT SLGFLESGS DPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPP
LGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPD
VHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQS SKLAAKWPTKLVKNCFLPLREYF
KYFSTELTSSLSGGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 35) dCas9-DNMT3a/31 (m) MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSNHDQEFDPPKVYPPVP
AEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQ
KHIQEWGPFDLVIGGSPCNDLS IVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFEN
VVAMGVSDKRDISRFLESNPVMIDAKEVS AAHRARYFWGNLPGMNRPLASTVNDKLELQECLE
HGRIAKFS KVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLAR
QRLLGRSWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMNPLEMFETVP
VWRRQPVRVLS LFEDIKKELT SLGFLESGS DPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPP
LGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPD
VHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQS SKLAAKWPTKLVKNCFLPLREYF
KYFSTELTSSLSGGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 36) dCas-DNMT3a/3L (h) without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSNHDQEFDPPKVYPPVP
AEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQ
KHIQEWGPFDLVIGGSPCNDLS IVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFEN
VVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLE
HGRIAKFS KVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLAR
QRLLGRSWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMNPLEMFETVP
VWRRQPVRVLS LFEDIKKELT SLGFLESGS DPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPP
LGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPD
VHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQS SKLAAKWPTKLVKNCFLPLREYF
KYFSTELTSSLSGGKRPAATKKAGQAKKKKGS (SEQ ID NO: 70) dCas9-DNMT3a/3L (m) without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSNHDQEFDPPKVYPPVP
AEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQ
KHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFEN
VVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLE
HGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLAR
QRLLGRSWSVPVIRHLFAPLKEYFACVSSGNSNANSRGPSFSSGLVPLSLRGSHMNPLEMFETVP
VWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPP
LGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPD
VHGGS LQNAVRVWS NIPAIRSRHWALV SEEELS LLAQNKQS SKLAAKWPTKLVKNCFLPLREYF
KYFSTELTSSLSGGKRPAATKKAGQAKKKKGS (SEQ ID NO: 71) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising G9A, e.g., a G9A
domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 26 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 26 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
G9A-dCas9 (MR-29387) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCTCGGGCGGGGGTGGCTCAGGAAA
TAGGGCTATCAGAACCGAGAAGATCATCTGTAGGGACGTGGCTAGAGGCTACGAGAACGTG
CCCATTCCTTGCGTGAATGGCGTGGATGGCGAACCTTGCCCCGAGGACTACAAATACATCTC
CGAGAACTGCGAAACCAGCACAATGAACATCGACAGAAACATCACCCACCTCCAGCACTGC
ACATGTGTGGATGACTGCTCCTCCAGCAACTGTCTGTGCGGCCAGCTCTCCATCAGATGCTG
GTACGACAAGGACGGCAGACTGCTGCAAGAGTTCAACAAGATCGAACCCCCTCTCATCTTCG
AGTGTAACCAAGCTTGCAGCTGCTGGAGAAACTGCAAGAATAGAGTGGTCCAGAGCGGCAT
CAAGGTGAGACTGCAACTGTACAGAACCGCCAAGATGGGATGGGGAGTGAGGGCTCTGCAA
ACCATTCCCCAAGGCACCTTCATCTGCGAATACGTGGGCGAACTGATCTCCGACGCCGAAGC
TGACGTGAGAGAGGACGACAGCTATCTCTTCGATCTGGACAATAAGGACGGCGAGGTGTAC
TGCATCGACGCTAGATATTACGGCAACATCTCTAGATTCATCAACCACCTCTGCGATCCCAA
CATCATTCCCGTGAGGGTGTTCATGCTGCACCAAGATCTGAGGTTCCCTAGAATCGCCTTCTT
CAGCTCTAGAGACATCAGAACCGGCGAGGAGCTGGGCTTCGATTACGGCGATAGATTCTGG
GACATCAAGTCCAAGTACTTCACATGCCAGTGCGGCAGCGAGAAGTGTAAGCACAGCGCTG
AGGCCATTGCTCTGGAGCAGTCTAGACTGGCCAGACTGGATGGAGGTGGCGGATCGGGAGA
CAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACC
GACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCA
TCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCG
GCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAG
GAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGA
GCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGA
CGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGAC
AGCACCGACAAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCG
GGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTC
ATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCG
TGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGAT
CGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTG
GGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGA
GCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGC
CGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGG
TGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCA
CCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAG
GAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCC
AGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCT
GCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGC
ATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTA
CCCCTTCCTGAAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACT
ACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGA
GACCATCACCCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTC
ATCGAGCGGATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACA
GCCTGCTGTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGA
GGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTG
TTCAAGACCAACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCG
AGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCAC
CTACCACGACCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAG
GACATCCTGGAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGG
AGCGGCTGAAAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCG
GCGGTACACCGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAG
AGCGGCAAGACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCA
GCTGATCCACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGC
CAGGGCGACAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGG
GCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCC
CGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAA
CAGCCGGGAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCT
GAAGGAGCACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTG
CAGAACGGCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACG
ACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCT
GACCCGGAGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAA
GAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTC
GACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCA
AGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCG
GATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTG
AAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCA
ACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAA
GAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGG
AAGATGATCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACA
GCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCG
GCCCCTGATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTC
GCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGC
AGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGC
CCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTAC
AGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAG
GAGCTGCTGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCT
GGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGC
CTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGG
GCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAG
AAGCTGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAG
CACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCG
ACGCCAACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGA
GCAGGCCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCA
AGTACTTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGC
CACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTG
GGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAG
AAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCT
GCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGG
TCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID
NO: 26) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 38 or 73. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 38 or 73 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
G9a-dCas9 MAPKKKRKVGIHGVPAASGGGGSGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYI
SENCETSTMNIDRNITHLQHCTCVDDCS S SNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQ
ACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVRED
D SYLFDLDNKDGEVYCIDARYYGNIS RFINHLCDPNIIPVRVFMLHQDLRFPRIAFFS S RD IRTGEE
LGFDYGDRFWDIKS KYFTCQCGSEKCKHSAEAIALEQSRLARLDGGGGSGD KKYS IGLAIGTNS V
GWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICY
LQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD
KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILS
ARLS KS RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKS NFDLAEDAKLQLS KDTYDDDLDNL
LAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL
PEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNG
SIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITP
WNFEEVVD KGASAQS FIERMTNFD KNLPNEKVLPKHS LLYEYFTVYNELTKVKYVTEGMRKPA
FLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDK
DFLDNEENED ILEDIVLTLTLFEDREMIEERLKTYAHLFDD KVMKQLKRRRYTGWGRLSRKLING
IRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGD SLHEHIANLAGSPAIKK
GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP
VENTQLQNEKLYLYYLQNGRDMYVD QELDINRLSDYDVAAIVPQSFLKDD S IDNKVLTRSD KA
RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQI
TKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNA
VVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGE
IRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIAR
KKDWDPKKYGGFD S PTVAYSVLVVAKVEKGKS KKLKSVKELLGITIMERS SFEKNPIDFLEAKG
YKEVKKDLIIKLPKYSLFELENGRKRMLAS AGELQKGNELALPSKYVNFLYLASHYEKLKGSPE
DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLT
NLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS QLGGDSGGKRPAATKKAG
QAKKKKGSYPYDVPDYA (SEQ ID NO: 38) G9A-dCas9 without HA tag MAPKKKRKVGIHGVPAASGGGGSGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYI
SENCETSTMNIDRNITHLQHCTCVDDCS SSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQ
ACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVRED
D SYLFDLDNKDGEVYCIDARYYGNIS RFINHLCDPNIIPVRVFMLHQDLRFPRIAFFS S RD IRTGEE
LGFDYGDRFWDIKS KYFTCQCGSEKCKHSAEAIALEQSRLARLDGGGGSGD KKYS IGLAIGTNS V
GWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICY
LQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTD
KADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILS
ARLS KSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKS NFDLAEDAKLQLSKDTYDDDLDNL
LAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQL
PEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNG
SIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITP
WNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPA
FLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDK
DFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLING
IRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKK
GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP
VENTQLQNEKLYLYYLQNGRDMYVD QELDINRLSDYDVAAIVPQSFLKDD S IDNKVLTRSDKA
RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQI
TKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNA
VVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGE
IRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIAR
KKDWDPKKYGGFD S PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERS SFEKNPIDFLEAKG
YKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE
DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLT
NLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS QLGGDSGGKRPAATKKAG
QAKKKKGS (SEQ ID NO: 73) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising HDAC8, e.g., a HDAC8 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 27 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 27 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-HDAC8 (MR-29439) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCT
CAGAGGAGCCCGAGGAGCCCGCCGATAGCGGACAATCTCTGGTGCCCGTCTACATCTACAG
CCCCGAATATGTGAGCATGTGTGATTCCCTCGCCAAGATCCCTAAGAGAGCCAGCATGGTGC
ATTCTCTGATCGAGGCCTACGCTCTGCATAAGCAAATGAGGATCGTGAAGCCCAAGGTCGCC
AGCATGGAAGAGATGGCCACCTTTCACACCGATGCCTACCTCCAACATCTCCAGAAGGTGTC
CCAAGAGGGCGACGACGACCACCCCGACTCCATTGAGTACGGACTGGGCTATGATTGCCCC
GCCACCGAGGGCATCTTTGACTATGCCGCCGCTATCGGCGGAGCTACCATCACAGCCGCCCA
GTGTCTGATTGATGGCATGTGCAAGGTCGCCATCAACTGGTCCGGAGGCTGGCATCATGCCA
AGAAGGATGAGGCCTCCGGCTTCTGTTATCTGAATGACGCCGTGCTGGGCATTCTGAGACTG
AGGAGGAAATTCGAGAGGATTCTGTACGTGGATCTGGATCTGCATCACGGAGATGGAGTCG
AAGATGCCTTCAGCTTCACCAGCAAGGTGATGACAGTCTCTCTGCACAAGTTCTCCCCCGGC
TTCTTTCCCGGAACCGGCGACGTGTCCGACGTGGGACTGGGCAAGGGAAGGTACTACAGCG
TGAACGTGCCCATTCAAGACGGCATCCAAGACGAGAAGTACTACCAGATCTGCGAGTCCGT
GCTCAAGGAGGTCTACCAAGCCTTCAATCCTAAGGCTGTCGTGCTCCAACTGGGAGCTGATA
CCATTGCTGGCGATCCCATGTGCAGCTTCAATATGACACCCGTCGGAATCGGCAAGTGCCTC
AAGTACATCCTCCAGTGGCAGCTCGCCACCCTCATTCTCGGAGGAGGCGGATACAATCTGGC
TAATACCGCCAGATGCTGGACCTATCTGACCGGCGTGATTCTGGGCAAAACACTGAGCAGCG
AAATCCCCGACCACGAGTTTTTCACCGCTTACGGCCCCGACTACGTGCTGGAGATCACCCCC
AGCTGCAGACCCGATAGAAACGAACCCCATAGAATCCAGCAAATTCTGAACTATATCAAGG
GCAACCTCAAGCACGTCGTGGGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCACCAAGAA
GGCCGGTCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGA
GCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCC
CTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA (SEQ ID NO: 27) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 39 or 74. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 39 or 74 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-HDAC8 MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNL SDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSEEPEEPAD SGQSLVPVYIYS PE
YVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEG
DDDHPD S IEYGLGYDCPATEGIFDYAAAIGGATITAAQCLID GMCKVAINWSGGWHHAKKDEAS
GFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVS
DVGLGKGRYYS VNVPIQDGIQDEKYYQICES VLKEVYQAFNPKAVVLQLGADTIAGDPMC SFN
MTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLS SEIPDHEFFTAYGPD
YVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVVGGGGSGKRPAATKKAGQAKKKKGSYPYDVP
DYA (SEQ ID NO: 39) dCas9-HDAC8 without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
.. QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGD S GGKRPAAT KKAGQAKKKKS GGGGSEEPEEPAD SGQSLVPVYIYS PE
YVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEG
DDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEAS
GFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVS
DVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFN
MTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPD
YVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVVGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID
NO: 74) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising LSD1, e.g., a LSD1 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 28 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 28 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-LSD1 (MR-29129) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGAGAGCGGCGGCGGCA
GCCTCTCCGGAAAGAAAGCCGCCGCTGCCGCTGCCGCCGCTGCTGCTGCCGCTACCGGCACA
GAAGCTGGCCCCGGAACAGCTGGCGGAAGCGAAAACGGAAGCGAAGTGGCTGCCCAGCCC
GCCGGACTGTCCGGACCCGCCGAGGTGGGACCCGGCGCTGTCGGCGAAAGGACCCCCAGAA
AAAAGGAGCCTCCTAGAGCCAGCCCTCCCGGCGGACTCGCCGAACCTCCCGGCAGCGCTGG
ACCTCAAGCCGGACCTACAGTGGTGCCCGGCAGCGCTACACCTATGGAGACCGGCATCGCT
GAGACCCCCGAGGGAAGGAGAACCAGCAGAAGGAAGAGAGCCAAGGTGGAGTACAGAGAG
ATGGATGAGTCTCTGGCTAACCTCAGCGAGGACGAGTACTACTCCGAGGAGGAAAGGAATG
CCAAGGCCGAGAAGGAGAAGAAGCTGCCCCCTCCTCCCCCTCAAGCCCCCCCCGAGGAGGA
GAACGAGTCCGAGCCCGAGGAACCCAGCGGAGTGGAAGGAGCCGCCTTTCAGTCCAGACTG
CCCCACGACAGAATGACATCCCAAGAGGCCGCTTGCTTTCCCGACATTATTTCCGGCCCTCA
GCAGACCCAGAAGGTGTTTCTGTTCATTAGAAATAGAACACTCCAGCTGTGGCTCGACAACC
CCAAGATCCAGCTGACCTTCGAGGCTACCCTCCAACAGCTGGAGGCCCCCTACAATAGCGAT
ACCGTGCTGGTGCACAGAGTGCACAGCTATCTGGAGAGGCACGGCCTCATTAACTTCGGCAT
TTACAAGCGGATCAAGCCCCTGCCCACCAAGAAAACAGGCAAGGTGATCATCATCGGCAGC
GGCGTGAGCGGCCTGGCCGCCGCCCGGCAGCTGCAGAGCTTCGGCATGGACGTGACCCTGC
TGGAGGCCCGGGACCGGGTGGGCGGCCGGGTGGCCACCTTCCGGAAGGGCAACTACGTGGC
CGACCTGGGCGCCATGGTGGTGACCGGCCTGGGCGGCAACCCCATGGCCGTGGTGAGCAAG
CAGGTGAACATGGAGCTGGCCAAGATCAAGCAGAAGTGCCCCCTGTACGAGGCCAACGGCC
AGGCCGTGCCCAAGGAGAAGGACGAGATGGTGGAGCAGGAGTTCAACCGGCTGCTGGAGG
CCACCAGCTACCTGAGCCACCAGCTGGACTTCAACGTGCTGAACAACAAGCACGTGAGCCT
GGGCCAGGCCCTGGAGGTGGTGATCCAGCTGCAGGAGAAGCACGTGAAGGACGAGCAGATC
GAGCACTGGAAGAAGATCGTGAAAACACAGGAGGAGCTGAAGGAGCTGCTGAACAAGATG
GTGAACCTGAAGGAGAAGATCAAGGAGCTGCACCAGCAGTACAAGGAGGCCAGCGAGGTG
AAGCCCCCCCGGGACATCACCGCCGAGTTCCTGGTCAAAAGCAAGCACCGGGACCTGACCG
CCCTGTGCAAGGAGTACGACGAGCTGGCCGAGACCCAGGGCAAGCTGGAGGAGAAGCTGCA
GGAGCTGGAGGCCAACCCCCCCAGCGACGTGTACCTGAGCAGCCGGGACCGGCAGATCCTG
GACTGGCACTTCGCCAACCTGGAGTTCGCCAACGCCACCCCCCTGAGCACCCTGAGCCTGAA
GCACTGGGACCAGGACGACGACTTCGAGTTCACCGGCAGCCACCTGACCGTGCGGAACGGC
TACAGCTGCGTGCCCGTGGCCCTGGCCGAGGGCCTGGACATCAAGCTGAACACCGCCGTGC
GGCAGGTGCGGTACACCGCCAGCGGCTGCGAGGTGATCGCCGTGAACACCCGGAGCACCAG
CCAGACCTTCATCTACAAGTGCGACGCCGTGCTGTGCACCCTGCCCCTGGGCGTGCTGAAGC
AGCAGCCCCCCGCCGTGCAGTTCGTGCCCCCCCTGCCCGAGTGGAAAACAAGCGCCGTGCA
GCGGATGGGCTTCGGCAACCTGAACAAGGTGGTGCTGTGCTTCGACCGGGTGTTCTGGGACC
CCAGCGTGAACCTGTTCGGCCACGTGGGCAGCACCACCGCCAGCCGGGGCGAGCTGTTCCTG
TTCTGGAACCTGTACAAGGCCCCCATCCTGCTGGCCCTGGTGGCCGGCGAGGCCGCCGGCAT
CATGGAGAACATCAGCGACGACGTGATCGTGGGCCGGTGCCTGGCCATCCTGAAGGGCATC
TTCGGCAGCAGCGCCGTGCCCCAGCCCAAGGAGACCGTGGTGAGCCGGTGGCGGGCCGACC
CCTGGGCCCGGGGCAGCTACAGCTACGTGGCCGCCGGCAGCAGCGGCAACGACTACGACCT
GATGGCCCAGCCCATCACCCCCGGCCCCAGCATCCCCGGCGCCCCCCAGCCCATCCCCCGGC
TGTTCTTCGCCGGCGAGCACACCATCCGGAACTACCCCGCCACCGTGCACGGCGCCCTGCTG
AGCGGCCTGCGGGAGGCAGGACGGATCGCCGACCAGTTCCTGGGCGCCATGTACACCCTGC
CCCGGCAGGCCACCCCCGGCGTGCCCGCCCAGCAGAGCCCCAGCATGAGCGGCGGCAAGCG
GCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGA
CGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTG
GCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGG
AAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 28) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 40 or 75. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 40 or 75, or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-LSD1 MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDD SIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLIT Q
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLD KVLSAYNKHRD KPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYT STKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKESGGGSLSGKKAAAAAAAAAAAATGT
EAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASPPGGLAEPPGSAGPQA
GPTVVPGSATPMETGIAETPEGRRTS RRKRAKVEYREMDES LANLSEDEYYSEEERNAKAEKEK
KLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTS QEAACFPDIISGPQQTQKVFLFIRNR
TLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVI
IIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAV
V SKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATS YLSHQLDFNVLNNKHVS
LGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPP
RD ITAEFLVKS KHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLS SRDRQILDWHFANL
EFANATPLS TLS LKHWD QDDDFEFTGSHLTVRNGYS CVPVALAEGLDIKLNTAVRQVRYTAS GC
EVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVL
CFDRVFWDPS VNLFGHVGSTTAS RGELFLFWNLYKAPILLALVAGEAAGIMENIS DDVIVGRCLA
ILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGS SGNDYDLMAQPITPGPSIPGAPQPIPR
LFFAGEHTIRNYPATVHGALLSGLREAGRIAD QFLGAMYTLPRQATPGVPA QQS PS MSGGKRPA
ATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 40) dCas9-LSD1 without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNS VGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYL QEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNL SDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDIN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKESGGGSLSGKKAAAAAAAAAAAATGT
EAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASPPGGLAEPPGSAGPQA
GPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLANLSEDEYYSEEERNAKAEKEK
KLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTS QEAACFPDIISGPQQTQKVFLFIRNR
TLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVI
IIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAV
VSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKHVS
LGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPP
RD ITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLS SRDRQILDWHFANL
EFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGC
EVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVL
CFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLA
ILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPR
LFFAGEHTIRNYPATVHGALLSGLREAGRIAD QFLGAMYTLPRQATPGVPA QQSPS MSGGKRPA
ATKKAGQAKKKKGS (SEQ ID NO: 75) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising EZH2, e.g., a EZH2 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 29 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 29 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
EZH2-dCas9 (MR-28938) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCGGCAGCGGCGGCAGCGGCCAGACCGGCAAGAAGAGCGAGAAGG
GCCCCGTGTGCTGGCGGAAGCGGGTGAAGAGCGAGTACATGCGGCTGCGGCAGCTGAAGCG
GTTCCGGCGGGCCGACGAGGTGAAGAGCATGTTCAGCAGCAACCGGCAGAAGATCCTGGAG
CGGACCGAGATCCTGAACCAGGAGTGGAAGCAGCGGCGAATCCAGCCCGTGCACATCCTGA
CCAGCGTGAGCAGCCTGCGGGGCACCCGGGAGTGCAGCGTGACCAGCGACCTGGACTTCCC
CACCCAGGTGATCCCCCTAAAGACCCTGAACGCCGTGGCCAGCGTGCCCATCATGTACAGCT
GGAGCCCCCTGCAGCAGAACTTCATGGTGGAGGACGAGACCGTGCTGCACAACATCCCCTA
CATGGGCGACGAGGTGCTGGACCAGGACGGCACCTTCATCGAGGAGCTGATCAAGAACTAC
GACGGCAAGGTGCACGGCGACCGGGAGTGCGGCTTCATCAACGACGAGATCTTCGTGGAGC
TGGTGAACGCCCTGGGCCAGTACAACGACGACGACGACGACGACGACGGCGACGACCCCGA
GGAGCGGGAGGAGAAGCAGAAGGACCTGGAGGACCACCGGGACGACAAGGAGAGCCGGCC
CCCCCGGAAGTTCCCCAGCGACAAGATCTTCGAGGCCATCAGCAGCATGTTCCCCGACAAGG
GCACCGCCGAGGAGCTGAAGGAGAAGTACAAGGAGCTGACCGAGCAGCAGCTGCCCGGCG
CCCTGCCCCCCGAGTGCACCCCCAACATCGACGGCCCCAACGCCAAGAGCGTGCAGCGGGA
GCAGAGCCTGCACAGCTTCCACACCCTGTTCTGCCGGCGGTGCTTCAAGTACGACTGCTTCC
TGCACCCCTTCCACGCCACCCCCAACACCTACAAGCGGAAGAACACCGAGACCGCCCTGGA
CAACAAGCCCTGCGGCCCCCAGTGCTACCAGCACCTGGAGGGCGCCAAGGAGTTCGCCGCC
GCCCTGACCGCCGAGCGGATCAAGACCCCCCCCAAGCGGCCCGGCGGCCGGCGGCGGGGCC
GGCTGCCCAACAACAGCAGCCGGCCCAGCACCCCCACCATCAACGTGCTGGAGAGCAAGGA
CACCGACAGCGACCGGGAGGCCGGCACCGAGACCGGCGGCGAGAACAACGACAAGGAGGA
GGAGGAGAAGAAGGACGAGACCAGCAGCAGCAGCGAGGCCAACAGCCGGTGCCAGACCCC
CATCAAGATGAAGCCCAACATCGAGCCCCCCGAGAACGTGGAGTGGAGCGGCGCCGAGGCC
AGCATGTTCCGGGTGCTGATCGGCACCTACTACGACAACTTCTGCGCCATCGCCCGGCTGAT
CGGCACCAAGACCTGCCGGCAGGTGTACGAGTTCCGGGTGAAGGAGAGCAGCATCATCGCC
CCCGCCCCCGCCGAGGACGTGGACACCCCCCCCCGGAAGAAGAAGCGGAAGCACCGGCTGT
GGGCCGCCCACTGCCGGAAGATCCAGCTGAAGAAGGACGGCAGCAGCAACCACGTGTACAA
CTACCAGCCCTGCGACCACCCCCGGCAGCCCTGCGACAGCAGCTGCCCCTGCGTGATCGCCC
AGAACTTCTGCGAGAAGTTCTGCCAGTGCAGCAGCGAGTGCCAGAACCGGTTCCCCGGCTGC
CGGTGCAAGGCCCAGTGCAACACCAAGCAGTGCCCCTGCTACCTGGCCGTGCGGGAGTGCG
ACCCCGACCTGTGCCTGACCTGCGGCGCCGCCGACCACTGGGACAGCAAGAACGTGAGCTG
CAAGAACTGCAGCATCCAGCGGGGCAGCAAGAAGCACCTGCTGCTGGCCCCCAGCGACGTG
GCCGGCTGGGGCATCTTCATCAAGGACCCCGTGCAGAAGAACGAGTTCATCAGCGAGTACT
GCGGCGAGATCATCAGCCAGGACGAGGCCGACCGGCGGGGCAAGGTGTACGACAAGTACAT
GTGCAGCTTCCTGTTCAACCTGAACAACGACTTCGTGGTGGACGCCACCCGGAAGGGCAACA
AGATCCGGTTCGCCAACCACAGCGTGAACCCCAACTGCTACGCCAAGGTGATGATGGTGAA
CGGCGACCACCGGATCGGCATCTTCGCCAAGCGGGCCATCCAGACCGGCGAGGAGCTGTTC
TTCGACTACCGGTACAGCCAGGCCGACGCCCTGAAGTACGTGGGCATCGAGCGGGAGATGG
AGATCCCCAGCACCGGCGGCAGCGGCGGCAGCGGCGGCAGCGGCGGCAGCGGCGGCAGCG
GCCGACCCGACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGC
CGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGAC
CGGCACAGCATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCG
AGGCCACCCGGCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTG
CTACCTGCAGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGC
TGGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAA
CATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAG
CTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGAT
CAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGAC
AAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGC
CAGCGGCGTGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAG
AACCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCC
TGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCT
GCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGAC
CAGTACGCCGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACAT
CCTGCGGGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTAC
GACGAGCACCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGA
AGTACAAGGAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGG
CGCCAGCCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACC
GAGGAGCTGCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACA
ACGGCAGCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGA
GGACTTCTACCCCTTCCTGAAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGG
ATCCCCTACTACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAA
ATCCGAGGAGACCATCACCCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCC
CAGAGCTTCATCGAGCGGATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGC
CCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTA
CGTGACCGAGGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTG
GACCTGCTGTTCAAGACCAACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCA
AGAAGATCGAGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAG
CCTGGGCACCTACCACGACCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAG
GAGAACGAGGACATCCTGGAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGA
TGATCGAGGAGCGGCTGAAAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCT
GAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGG
GACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGA
ACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCA
GGTGAGCGGCCAGGGCGACAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCC
ATCAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCC
GGCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGG
GCCAGAAGAACAGCCGGGAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCA
GCCAGATCCTGAAGGAGCACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCT
GTACTACCTGCAGAACGGCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTG
AGCGACTACGACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACA
ACAAGGTGCTGACCCGGAGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGG
AGGTGGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCA
GCGGAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCC
GGCTTCATCAAGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCC
TGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGG
TGATCACCCTGAAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTG
CGGGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCG
CCCTGATCAAGAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTA
CGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTA
CTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGA
TCCGGAAGCGGCCCCTGATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGG
CCGGGACTTCGCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAA
ACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACA
AGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCAC
CGTGGCCTACAGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAA
ATCCGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCC
ATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGC
CCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGA
GCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCC
AGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGG
AGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGT
GATCCTGGCCGACGCCAACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAG
CCCATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCC
CGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAG
GTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCT
GAGCCAGCTGGGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCA
GGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGCGGCCGCT
TAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTG
TACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AA (SEQ ID NO: 29) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 41 or 76. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 41 or 76 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
EZH2-dCas9 MAPKKKRKVGGSGGSGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQK
ILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSP
LQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALG
QYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEK
YKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKR
KNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVL
ESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHC
RKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCN
TKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDP
VQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIPSTGGSGGSGGSGG
SGGSGRPDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA
EATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQ
TYNQLFEENPINASGVDAKAILSARLS KS RRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNF
DLAEDAKLQLS KDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGASQEEFYKFIKPILEKMDGT
EELLVKLNREDLLRKQRTFDNGSIPH QIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
PLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM
KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNS
RERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVAAI
VPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKT
EV QTGGFS KESILPKRNS D KLIARKKDWDPKKYGGFD SPTVAYSVLVVAKVEKGKS KKLKSVKE
LLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLAS AGELQKGNELAL
PS KYVNFLYLAS HYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQIS EFS KRVILADANLD KVLS A
YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGDSGGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 41) EZH2-dCas9 without HA tag MAPKKKRKVGGSGGSGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFS SNRQK
ILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSP
LQQNFMVEDETVLHNIPYMGDEVLD QDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALG
QYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEK
YKELTEQQLPGALPPECTPNIDGPNAKS VQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKR
KNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNS SRPSTPTINVL
ES KDTD SDREAGTETGGENND KEEEEKKDET S S S SEANS RC QTPIKMKPNIEPPENVEWS GAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHC
RKIQLKKDGS SNHVYNYQPCDHPRQPCDS SCPCVIAQNFCEKFCQCS SEC QNRFPGCRCKAQCN
TKQCPCYLAVRECDPDLCLTCGAADHWD S KNV SC KNC SIQRGS KKHLLLAPSDVAGWGIFIKDP
V QKNEFISEYCGEIIS QDEADRRGKVYD KYMCS FLFNLNNDFVVDATRKGN KIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIPSTGGSGGSGGSGG
SGGSGRPDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA
EATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQ
TYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNF
DLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKFIKPILEKMDGT
EELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
PLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNAS LGTYHDLLKIIKDKDFLDNEENEDILED IVLTLTLFEDREMIEERLKTYAHLFDDKVM
KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNS
RERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVAAI
VPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKT
EVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKE
LLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLAS AGELQKGNELAL
PSKYVNFLYLAS HYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQIS EFS KRVILADANLDKVLS A
YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGDSGGKRPAATKKAGQAKKKKGS (SEQ ID NO: 76) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a first repressor domain comprising EZH2 e.g., a EZH2 domain and a second repressor domain comprising KRAB, e.g., a KRAB domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 30 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 30 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
EZH2- dCas9-KRAB (MR-29448) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCGGCAGCGGCGGCAGCGGCCAGACCGGCAAGAAGAGCGAGAAGG
GCCCCGTGTGCTGGCGGAAGCGGGTGAAGAGCGAGTACATGCGGCTGCGGCAGCTGAAGCG
GTTCCGGCGGGCCGACGAGGTGAAGAGCATGTTCAGCAGCAACCGGCAGAAGATCCTGGAG
CGGACCGAGATCCTGAACCAGGAGTGGAAGCAGCGGCGAATCCAGCCCGTGCACATCCTGA
CCAGCGTGAGCAGCCTGCGGGGCACCCGGGAGTGCAGCGTGACCAGCGACCTGGACTTCCC
CACCCAGGTGATCCCCCTAAAGACCCTGAACGCCGTGGCCAGCGTGCCCATCATGTACAGCT
GGAGCCCCCTGCAGCAGAACTTCATGGTGGAGGACGAGACCGTGCTGCACAACATCCCCTA
CATGGGCGACGAGGTGCTGGACCAGGACGGCACCTTCATCGAGGAGCTGATCAAGAACTAC
GACGGCAAGGTGCACGGCGACCGGGAGTGCGGCTTCATCAACGACGAGATCTTCGTGGAGC
TGGTGAACGCCCTGGGCCAGTACAACGACGACGACGACGACGACGACGGCGACGACCCCGA
GGAGCGGGAGGAGAAGCAGAAGGACCTGGAGGACCACCGGGACGACAAGGAGAGCCGGCC
CCCCCGGAAGTTCCCCAGCGACAAGATCTTCGAGGCCATCAGCAGCATGTTCCCCGACAAGG
GCACCGCCGAGGAGCTGAAGGAGAAGTACAAGGAGCTGACCGAGCAGCAGCTGCCCGGCG
CCCTGCCCCCCGAGTGCACCCCCAACATCGACGGCCCCAACGCCAAGAGCGTGCAGCGGGA
GCAGAGCCTGCACAGCTTCCACACCCTGTTCTGCCGGCGGTGCTTCAAGTACGACTGCTTCC
TGCACCCCTTCCACGCCACCCCCAACACCTACAAGCGGAAGAACACCGAGACCGCCCTGGA
CAACAAGCCCTGCGGCCCCCAGTGCTACCAGCACCTGGAGGGCGCCAAGGAGTTCGCCGCC
GCCCTGACCGCCGAGCGGATCAAGACCCCCCCCAAGCGGCCCGGCGGCCGGCGGCGGGGCC
GGCTGCCCAACAACAGCAGCCGGCCCAGCACCCCCACCATCAACGTGCTGGAGAGCAAGGA
CACCGACAGCGACCGGGAGGCCGGCACCGAGACCGGCGGCGAGAACAACGACAAGGAGGA
GGAGGAGAAGAAGGACGAGACCAGCAGCAGCAGCGAGGCCAACAGCCGGTGCCAGACCCC
CATCAAGATGAAGCCCAACATCGAGCCCCCCGAGAACGTGGAGTGGAGCGGCGCCGAGGCC
AGCATGTTCCGGGTGCTGATCGGCACCTACTACGACAACTTCTGCGCCATCGCCCGGCTGAT
CGGCACCAAGACCTGCCGGCAGGTGTACGAGTTCCGGGTGAAGGAGAGCAGCATCATCGCC
CCCGCCCCCGCCGAGGACGTGGACACCCCCCCCCGGAAGAAGAAGCGGAAGCACCGGCTGT
GGGCCGCCCACTGCCGGAAGATCCAGCTGAAGAAGGACGGCAGCAGCAACCACGTGTACAA
CTACCAGCCCTGCGACCACCCCCGGCAGCCCTGCGACAGCAGCTGCCCCTGCGTGATCGCCC
AGAACTTCTGCGAGAAGTTCTGCCAGTGCAGCAGCGAGTGCCAGAACCGGTTCCCCGGCTGC
CGGTGCAAGGCCCAGTGCAACACCAAGCAGTGCCCCTGCTACCTGGCCGTGCGGGAGTGCG
ACCCCGACCTGTGCCTGACCTGCGGCGCCGCCGACCACTGGGACAGCAAGAACGTGAGCTG
CAAGAACTGCAGCATCCAGCGGGGCAGCAAGAAGCACCTGCTGCTGGCCCCCAGCGACGTG
GCCGGCTGGGGCATCTTCATCAAGGACCCCGTGCAGAAGAACGAGTTCATCAGCGAGTACT
GCGGCGAGATCATCAGCCAGGACGAGGCCGACCGGCGGGGCAAGGTGTACGACAAGTACAT
GTGCAGCTTCCTGTTCAACCTGAACAACGACTTCGTGGTGGACGCCACCCGGAAGGGCAACA
AGATCCGGTTCGCCAACCACAGCGTGAACCCCAACTGCTACGCCAAGGTGATGATGGTGAA
CGGCGACCACCGGATCGGCATCTTCGCCAAGCGGGCCATCCAGACCGGCGAGGAGCTGTTC
TTCGACTACCGGTACAGCCAGGCCGACGCCCTGAAGTACGTGGGCATCGAGCGGGAGATGG
AGATCCCCAGCACCGGCGGCAGCGGCGGCAGCGGCGGCAGCGGCGGCAGCGGCGGCAGCG
GCCGACCCGACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGC
CGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGAC
CGGCACAGCATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCG
AGGCCACCCGGCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTG
CTACCTGCAGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGC
TGGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAA
CATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAG
CTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGAT
CAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGAC
AAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGC
CAGCGGCGTGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAG
AACCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCC
TGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCT
GCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGAC
CAGTACGCCGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACAT
CCTGCGGGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTAC
GACGAGCACCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGA
AGTACAAGGAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGG
CGCCAGCCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACC
GAGGAGCTGCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACA
ACGGCAGCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGA
GGACTTCTACCCCTTCCTGAAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGG
ATCCCCTACTACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAA
ATCCGAGGAGACCATCACCCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCC
CAGAGCTTCATCGAGCGGATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGC
CCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTA
CGTGACCGAGGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTG
GACCTGCTGTTCAAGACCAACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCA
AGAAGATCGAGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAG
CCTGGGCACCTACCACGACCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAG
GAGAACGAGGACATCCTGGAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGA
TGATCGAGGAGCGGCTGAAAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCT
GAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGG
GACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGA
ACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCA
GGTGAGCGGCCAGGGCGACAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCC
ATCAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCC
GGCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGG
GCCAGAAGAACAGCCGGGAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCA
GCCAGATCCTGAAGGAGCACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCT
GTACTACCTGCAGAACGGCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTG
AGCGACTACGACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACA
ACAAGGTGCTGACCCGGAGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGG
AGGTGGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCA
GCGGAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCC
GGCTTCATCAAGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCC
TGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGG
TGATCACCCTGAAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTG
CGGGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCG
CCCTGATCAAGAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTA
CGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTA
CTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGA
TCCGGAAGCGGCCCCTGATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGG
CCGGGACTTCGCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAA
ACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACA
AGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCAC
CGTGGCCTACAGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAA
ATCCGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCC
ATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGC
CCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGA
GCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCC
AGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGG
AGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGT
GATCCTGGCCGACGCCAACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAG
CCCATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCC
CGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAG
GTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCT
GAGCCAGCTGGGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCA
GGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCTCAGACGCTAAGTCTCTGACCGCTTGGAGC
AGAACACTGGTCACCTTCAAGGACGTGTTCGTCGACTTCACAAGAGAGGAGTGGAAACTGC
TGGACACCGCCCAGCAGATCCTCTATAGAAACGTCATGCTGGAGAACTACAAGAATCTGGT
GTCTCTGGGCTACCAGCTGACCAAGCCCGACGTGATTCTGAGGCTGGAGAAGGGCGAGGAG
CCTTGGCTGGTGGAGAGAGAGATCCACCAAGAAACCCACCCCGACAGCGAAACCGCCTTCG
AGATCAAGAGCAGCGTGGGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCACCAAGAAGG
CCGGTCAGGCCAAGAAGAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGC
GGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTT
GCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA (SEQ ID NO: 30) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 42 or 77. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 42 or 77 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
EZH2-dCas9-KRAB
MAPKKKRKVGGSGGSGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQK
ILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSP
LQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALG
QYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEK
YKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKR
KNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVL
ESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHC
RKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCN
TKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDP
V QKNEFISEYCGEIIS QDEADRRGKVYD KYMCS FLFNLNNDFVVDATRKGN KIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIPSTGGSGGSGGSGG
SGGSGRPDKKYSIGLAIGTNSVGWAVITDEYKVPS KKFKVLGNTDRHSIKKNLIGALLFDSGETA
EATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQ
TYNQLFEENPINASGVDAKAILSARLS KS RRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNF
DLAEDAKLQLS KDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGASQEEFYKFIKPILEKMDGT
EELLVKLNREDLLRKQRTFDNGSIPH QIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
PLARGNSRFAWMTRKSEETITPWNFEEVVD KGASAQS FIERMTNFD KNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM
KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNS
RERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVAAI
VPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKT
EV QTGGFS KESILPKRNS D KLIARKKDWDPKKYGGFD SPTVAYSVLVVAKVEKGKS KKLKSVKE
LLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLAS AGELQKGNELAL
PS KYVNFLYLAS HYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQIS EFS KRVILADANLD KVLS A
YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGDSGGKRPAATKKAGQAKKKKSGGGGSDAKSLTAWSRTLVTFKDVFVDFTREEWKL
LDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIKS
SVGGGGSGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 42) EZH2-dCas9-KRAB without HA tag MAPKKKRKVGGSGGSGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFS SNRQK
ILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSP
LQQNFMVEDETVLHNIPYMGDEVLD QDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALG
QYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEK
YKELTEQQLPGALPPECTPNIDGPNAKS VQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKR
KNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNS SRPSTPTINVL
ESKDTDSDREAGTETGGENNDKEEEEKKDETSSS SEANSRCQTPIKMKPNIEPPENVEWSGAEAS
MFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHC
RKIQLKKDGS SNHVYNYQPCDHPRQPCDS SCPCVIAQNFCEKFCQCS SECQNRFPGCRCKAQCN
TKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDP
V QKNEFISEYCGEIIS QDEADRRGKVYD KYMCS FLFNLNNDFVVDATRKGNKIRFANHSVNPNC
YAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIPSTGGSGGSGGSGG
SGGSGRPDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETA
EATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDE
VAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQ
TYNQLFEENPINAS GVDAKAILS ARLS KS RRLENLIAQLPGEKKNGLFGNLIALS LGLTPNFKSNF
DLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKFIKPILEKMDGT
EELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
PLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM
KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNS
RERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVAAI
VPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKA
TAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKT
EVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKE
LLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKRMLAS AGELQKGNELAL
PSKYVNFLYLAS HYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQIS EFS KRVILADANLD KVLS A
YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGDSGGKRPAATKKAGQAKKKKSGGGGSDAKSLTAWSRTLVTFKDVFVDFTREEWKL
LDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIKS
SVGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID NO: 77) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a first repressor domain comprising G9A e.g., a G9A domain and a second repressor domain comprising KRAB, e.g., a KRAB domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 31 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 31 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
G9A-dCas9-KRAB (MR-29442) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCTCGGGCGGGGGTGGCTCAGGAAG
CGCCGCTATCGCCGAAGTGCTGCTGAATGCCAGATGCGATCTGCATGCCGTGAACTACCACG
GCGACACCCCTCTGCATATCGCCGCTAGAGAGAGCTACCATGACTGTGTGCTGCTGTTTCTG
AGCAGAGGCGCCAATCCCGAGCTCAGAAACAAAGAGGGCGACACCGCTTGGGATCTGACAC
CCGAGAGATCCGACGTGTGGTTCGCTCTGCAACTGAATAGAAAACTGAGACTGGGCGTCGG
CAATAGGGCCATTAGAACCGAGAAGATCATCTGTAGGGACGTGGCTAGGGGCTACGAGAAC
GTGCCCATCCCTTGTGTGAACGGAGTGGATGGAGAGCCTTGCCCCGAGGATTACAAATACAT
CAGCGAGAACTGCGAAACCTCCACCATGAATATCGATAGAAACATTACACACCTCCAGCAC
TGTACATGCGTGGACGATTGCAGCAGCAGCAACTGTCTGTGCGGCCAACTGAGCATCAGATG
CTGGTACGACAAGGATGGCAGACTGCTGCAAGAGTTCAACAAGATCGAACCCCCTCTGATCT
TCGAGTGTAACCAAGCTTGCAGCTGTTGGAGGAACTGCAAGAATAGGGTCGTGCAGTCCGG
AATCAAGGTGAGACTGCAGCTGTATAGAACAGCTAAGATGGGATGGGGAGTCAGAGCTCTG
CAGACCATCCCCCAAGGCACATTCATCTGTGAGTACGTCGGCGAACTCATCAGCGACGCTGA
GGCCGATGTGAGGGAGGACGACAGCTATCTCTTCGACCTCGACAACAAGGACGGCGAGGTG
TACTGCATCGACGCTAGATATTACGGCAACATCAGCAGATTCATCAACCACCTCTGCGACCC
CAATATCATCCCCGTGAGAGTGTTCATGCTCCATCAAGATCTGAGATTCCCTAGGATCGCCTT
CTTCAGCTCTAGAGACATTAGAACCGGCGAGGAGCTGGGATTCGACTACGGCGACAGGTTCT
GGGACATCAAGAGCAAGTACTTCACATGCCAATGCGGCAGCGAGAAATGCAAGCATAGCGC
CGAGGCCATTGCTCTGGAGCAGTCTAGACTGGCTAGGCTGGACCCTCACCCCGAGCTGCTGC
CCGAACTGGGATCTCTGCCTCCCGTGAATACCGGAGGTGGCGGATCGGGAGACAAGAAGTA
CAGCATCGGCCTGGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTAC
AAGGTGCCCAGCAAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGA
ACCTGATCGGCGCCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCG
GACCGCCCGGCGGCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTC
AGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGG
TGGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGC
CTACCACGAGAAGTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGAC
AAGGCCGACCTGCGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTT
CCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTG
GTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCA
AGGCCATCCTGAGCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCT
GCCCGGCGAGAAGAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACC
CCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACA
CCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTC
CTGGCCGCCAAGAACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCG
AGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGA
CCTGACCCTGCTGAAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCT
TCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTT
CTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAG
CTGAACCGGGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACC
AGATCCACCTGGGCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTG
AAGGACAACCGGGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCC
CCCTGGCCCGGGGCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCAC
CCCCTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGG
ATGACCAACTTCGACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGT
ACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCG
GAAGCCCGCCTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACC
AACCGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCG
ACAGCGTGGAGATCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGA
CCTGCTGAAGATCATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTG
GAGGACATCGTGCTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGA
AAACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACAC
CGGCTGGGGCCGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAA
GACCATCCTGGACTTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCC
ACGACGACAGCCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGA
CAGCCTGCACGAGCACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTG
CAGACCGTGAAGGTGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAAC
ATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGG
GAGCGGATGAAGCGGATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAG
CACCCCGTGGAGAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACG
GCCGGGACATGTACGTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGC
CGCCATCGTGCCCCAGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGG
AGCGACAAGGCCCGGGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATG
AAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACC
TGACCAAGGCCGAGCGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCA
GCTGGTGGAGACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAAC
ACCAAGTACGACGAGAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCA
AGCTGGTGAGCGACTTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTA
CCACCACGCCCACGACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTAC
CCCAAGCTGGAGAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGA
TCGCCAAGAGCGAGCAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACAT
CATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTG
ATCGAGACCAACGGCGAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCG
TGCGGAAGGTGCTGAGCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGG
CGGCTTCAGCAAGGAGAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAG
AAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGC
TGGTGGTGGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGC
TGGGCATCACCATCATGGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGC
CAAGGGCTACAAGGAGGTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTC
GAGCTGGAGAACGGCCGGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAAC
GAGCTGGCCCTGCCCAGCAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCT
GAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTA
CCTGGACGAGATCATCGAGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCC
AACCTGGACAAGGTGCTGAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGG
CCGAGAACATCATCCACCTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTAC
TTCGACACCACCATCGACCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCC
TGATCCACCAGAGCATCACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGG
CGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAA
GTCGGGCGGGGGTGGCTCAGACGCTAAGTCTCTGACCGCTTGGAGCAGAACACTGGTCACCT
TCAAGGACGTGTTCGTCGACTTCACAAGAGAGGAGTGGAAACTGCTGGACACCGCCCAGCA
GATCCTCTATAGAAACGTCATGCTGGAGAACTACAAGAATCTGGTGTCTCTGGGCTACCAGC
TGACCAAGCCCGACGTGATTCTGAGGCTGGAGAAGGGCGAGGAGCCTTGGCTGGTGGAGAG
AGAGATCCACCAAGAAACCCACCCCGACAGCGAAACCGCCTTCGAGATCAAGAGCAGCGTG
GGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCACCAAGAAGGCCGGTCAGGCCAAGAAG
AAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCT
GCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGG
TCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID
NO: 31) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 43 or 78. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 42 or 77 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having .. no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
G9A-dCas9-KRAB
MAPKKKRKVGIHGVPAASGGGGSGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVL
LFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYEN
VPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYD
KDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQG
TFICEYVGELISDAEAD VREDD SYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFML
HQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLD
PHPELLPELGS LPPVNTGGGGS GDKKYSIGLAIGTNSVGWAVITDEYKVPS KKFKVLGNTDRHSI
KKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEE
DKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDL
NPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFG
NLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSD
ILRVNTEITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS Q
EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKD
NREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQL
KEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREM
IEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIV
IEMARENQTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENTQLQNEKLYLYYLQNGRDMYV
DQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLL
NAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIRE
VKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFAT
VRKVLSMPQVNIVKKTEVQTGGFS KESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKR
MLASAGELQKGNELALPS KYVNFLYLAS HYEKLKGS PEDNEQKQLFVEQHKHYLDEIIEQIS EFS
KRVILADANLD KVLSAYNKHRD KPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYT STKEVL
DATLIHQSITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSDAKSLTAWSRTLVT
FKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREI
HQETHPDSETAFEIKSSVGGGGSGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 43) G9A-dCas9-KRAB without HA tag MAPKKKRKVGIHGVPAASGGGGSGS AAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVL
LFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYEN
VPIPCVNGVDGEPCPEDYKYISENCETS TMNIDRNITHLQHCTCVDDC S S SNCLCGQLSIRCWYD
KDGRLLQEFNKIEPPLIFECNQACS CWRNC KNRVV QSGIKVRLQLYRTAKMGWGVRALQTIPQG
TFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFML
HQDLRFPRIAFFS SRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLD
PHPELLPELGS LPPVNTGGGGS GD KKYSIGLAIGTNSVGWAVITDEYKVPS KKFKVLGNTDRHSI
KKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEE
DKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDL
NPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFG
NLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSD
ILRVNTEITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGASQ
EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKD
NREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQL
KEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREM
IEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIV
IEMARENQTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENTQLQNEKLYLYYLQNGRDMYV
DQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLL
NAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIRE
VKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFAT
VRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYS LFELENGRKR
MLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFS
KRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSDAKSLTAWSRTLVT
FKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREI
HQETHPDSETAFEIKSSVGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID NO: 78) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a first repressor domain comprising FOG1 e.g., a FOG1 domain and a second repressor domain comprising FOG1, e.g., a FOG1 domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 32 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 32 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
FOG1-dCas9-FOG1 (MR-29434) AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCTCGGGCGGGGGTGGCTCAAGCAG
AAGGAAGCAGAGCAACCCCAGACAAATCAAGAGATCTCTGGGCGACATGGAGGCCAGAGA
GGAAGTGCAGCTGGTGGGCGCCAGCCACATGGAGCAGAAGGCTACAGCCCCCGAGGCCCCC
AGCCCCGGAGGTGGCGGATCGGGAGACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCA
ACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAGTTCAAGGT
GCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGAC
AGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCGGCGGTACACCCGGC
GGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGA
CAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGG
CACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTA
CCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTACCTG
GCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGA
CAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAG
GAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGAGCGCCCGGCTGAGCA
AGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAACGGCCTGTT
CGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGG
CCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCT
GGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGAACCTGAGCGACGCC
ATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCA
GCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTGAAGGCCCTGGTGCG
GCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCAAGAACGGCTACGCC
GGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGG
AGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAGGACCTGCTGCGGA
AGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGC
CATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGGGAGAAGATCGAG
AAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGGGCAACAGCCGGTT
CGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTTCGAGGAGGTGGTG
GACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGACAAGAACCTGC
CCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTACAACGA
GCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTTCCTGAGCGGCGAG
CAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTGACCGTGAAGCAGC
TGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGATCAGCGGCGTGGA
GGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGATCATCAAGGACAAG
GACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTGCTGACCCTGACCC
TGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCCACCTGTTCGACGA
CAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTGAGCCGGAA
GCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTGAAATCC
GACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTCAAGGA
GGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAGCACATCGCCAAC
CTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTGGACGAGC
TGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAA
CCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCGGATCGAGGAGGG
CATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAACACCCAGCTGCAG
AACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTACGTGGACCAGGAGC
TGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCCAGAGCTTCCTGAAG
GACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCGGGGCAAGAGCGAC
AACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACG
CCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGAG
CGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCCGGCAGATCACCAAG
CACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGA
TCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGACTTCCGGAAGGACTT
CCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAAC
GCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAGCGAGTTCGTGTACG
GCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAGATCGGCA
AGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACC
CTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGCGAGACCGGCGAGA
TCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGAGCATGCCCCAGGT
GAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGAGCATCCTGCCC
AAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGC
GGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCA
AGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGAGCAG
CTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGAC
CTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCCGGAAGCGGATGC
TGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAGCAAGTACGTGAA
CTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAGGACAACGAGCAG
AAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCG
AGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCTGAGCGCCTACAA
CAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACCCTG
ACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAAGCGGTA
CACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTAC
GAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCA
CCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCTCAAGCAGAAGGA
AGCAGAGCAACCCCAGACAAATCAAGAGATCTCTGGGCGACATGGAGGCCAGAGAGGAAG
TGCAGCTGGTGGGCGCCAGCCACATGGAGCAGAAGGCTACAGCCCCCGAGGCCCCCAGCCC
CGGAGGTGGCGGATCGGGAAAGCGGCCCGCCGCCACCAAGAAGGCCGGTCAGGCCAAGAA
GAAGAAGGGCAGCTACCCCTACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGC
TGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTG
GTCTTTGAATAAAGCCTGAGTAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID
NO: 32) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 44 or 79. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 44 or 79 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
FOG1-dCas9-FOG1 MAPKKKRKVGIHGVPAASGGGGSSRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPE
APS PGGGGS GD KKYSIGLAIGTNS VGWAVITDEYKVPSKKFKVLGNTDRHS IKKNLIGALLFD S G
ETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI
VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQ
LV QTYNQLFEENPINAS GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFK
S NFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILL SD ILRVNTEIT KAPLS
ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLL
YEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSV
EISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDD
KVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQ
KAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYD
VAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLT
KAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDF
RKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI
GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNI
VKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKL
KSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AGELQKG
NELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDK
VLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLY
ETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGS SRRKQSNPRQIKRSLGDMEAREEVQL
VGASHMEQKATAPEAPSPGGGGSGKRPAATKKAGQAKKKKGSYPYDVPDYA (SEQ ID NO: 44) FOG1-dCas9-FOG1 without HA tag MAPKKKRKVGIHGVPAASGGGGSSRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPE
APS PGGGGS GD KKYSIGLAIGTNS VGWAVITDEYKVPSKKFKVLGNTDRHS IKKNLIGALLFD S G
ETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI
VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQ
LVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFK
S NFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILL SD ILRVNTEIT KAPLS
ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKM
DGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLL
YEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSV
EISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDD
KVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQ
KAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYD
VAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLT
KAERGGLSELDKAGFIKRQLVETRQITKHVAQILD SRMNTKYDENDKLIREVKVITLKS KLVS DF
RKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI
GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNI
VKKTEV QTGGFS KESILPKRNSDKLIARKKDWDPKKYGGFD S PTVAYSVLVVAKVEKGKSKKL
KSV KELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AGELQKG
NELALPS KYVNFLYLAS HYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQIS EFSKRVILADANLDK
VLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLY
ETRIDLS QLGGD S GGKRPAAT KKAGQAKKKKSGGGGS SRRKQSNPRQIKRS LGDMEAREEVQL
VGASHMEQKATAPEAPSPGGGGSGKRPAATKKAGQAKKKKGS (SEQ ID NO: 79) In some embodiments, an expression repressor comprises a DNA-targeting moiety comprising dCas9, e.g., an S. pyogenes dCas9, and a repressor domain comprising DNMT, e.g., a DNMT3b domain. In some embodiments, the expression repressor is encoded by the nucleic acid sequence of SEQ ID NOs: 15 (e.g., a nucleic acid (e.g., cDNA) encoding the expression repressor). In some embodiments, a nucleic acid described herein comprises a nucleic acid sequence of SEQ ID NO: 15 or a sequence with at least 80, 85, 90, 95, 99, or 100% identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT3b AGGAAATAAGAGAGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATGGCCCCCAAGA
AGAAGCGGAAGGTGGGCATCCACGGCGTGCCCGCCGCCGACAAGAAGTACAGCATCGGCCT
GGCCATCGGCACCAACAGCGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGC
AAGAAGTTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGCG
CCCTGCTGTTCGACAGCGGCGAGACCGCCGAGGCCACCCGGCTGAAGCGGACCGCCCGGCG
GCGGTACACCCGGCGGAAGAACCGGATCTGCTACCTGCAGGAGATCTTCAGCAACGAGATG
GCCAAGGTGGACGACAGCTTCTTCCACCGGCTGGAGGAGAGCTTCCTGGTGGAGGAGGACA
AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
GTACCCCACCATCTACCACCTGCGGAAGAAGCTGGTGGACAGCACCGACAAGGCCGACCTG
CGGCTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGG
CGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTAC
AACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGA
GCGCCCGGCTGAGCAAGAGCCGGCGGCTGGAGAACCTGATCGCCCAGCTGCCCGGCGAGAA
GAAGAACGGCCTGTTCGGCAACCTGATCGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGA
GCAACTTCGACCTGGCCGAGGACGCCAAGCTGCAGCTGAGCAAGGACACCTACGACGACGA
CCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGA
ACCTGAGCGACGCCATCCTGCTGAGCGACATCCTGCGGGTGAACACCGAGATCACCAAGGC
CCCCCTGAGCGCCAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTG
AAGGCCCTGGTGCGGCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGAGCA
AGAACGGCTACGCCGGCTACATCGACGGCGGCGCCAGCCAGGAGGAGTTCTACAAGTTCAT
CAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTGAAGCTGAACCGGGAG
GACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGG
GCGAGCTGCACGCCATCCTGCGGCGGCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCG
GGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCCTACTACGTGGGCCCCCTGGCCCGGG
GCAACAGCCGGTTCGCCTGGATGACCCGGAAATCCGAGGAGACCATCACCCCCTGGAACTT
CGAGGAGGTGGTGGACAAGGGCGCCAGCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
GACAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCA
CCGTGTACAACGAGCTGACCAAGGTGAAGTACGTGACCGAGGGCATGCGGAAGCCCGCCTT
CCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAGGTG
ACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACAGCGTGGAGA
TCAGCGGCGTGGAGGACCGGTTCAACGCCAGCCTGGGCACCTACCACGACCTGCTGAAGAT
CATCAAGGACAAGGACTTCCTGGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTG
CTGACCCTGACCCTGTTCGAGGACCGGGAGATGATCGAGGAGCGGCTGAAAACCTACGCCC
ACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGGCGGTACACCGGCTGGGGCCG
GCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGAC
TTCCTGAAATCCGACGGCTTCGCCAACCGGAACTTCATGCAGCTGATCCACGACGACAGCCT
GACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGAGCGGCCAGGGCGACAGCCTGCACGAG
CACATCGCCAACCTGGCCGGCAGCCCCGCCATCAAGAAGGGCATCCTGCAGACCGTGAAGG
TGGTGGACGAGCTGGTGAAGGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAGAT
GGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGATGAAGCG
GATCGAGGAGGGCATCAAGGAGCTGGGCAGCCAGATCCTGAAGGAGCACCCCGTGGAGAA
CACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAACGGCCGGGACATGTAC
GTGGACCAGGAGCTGGACATCAACCGGCTGAGCGACTACGACGTGGCCGCCATCGTGCCCC
AGAGCTTCCTGAAGGACGACAGCATCGACAACAAGGTGCTGACCCGGAGCGACAAGGCCCG
GGGCAAGAGCGACAACGTGCCCAGCGAGGAGGTGGTGAAGAAGATGAAGAACTACTGGCG
GCAGCTGCTGAACGCCAAGCTGATCACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAG
CGGGGCGGCCTGAGCGAGCTGGACAAGGCCGGCTTCATCAAGCGGCAGCTGGTGGAGACCC
GGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGA
GAACGACAAGCTGATCCGGGAGGTGAAGGTGATCACCCTGAAATCCAAGCTGGTGAGCGAC
TTCCGGAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACG
ACGCCTACCTGAACGCCGTGGTGGGCACCGCCCTGATCAAGAAGTACCCCAAGCTGGAGAG
CGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAG
CAGGAGATCGGCAAGGCCACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAA
GACCGAGATCACCCTGGCCAACGGCGAGATCCGGAAGCGGCCCCTGATCGAGACCAACGGC
GAGACCGGCGAGATCGTGTGGGACAAGGGCCGGGACTTCGCCACCGTGCGGAAGGTGCTGA
GCATGCCCCAGGTGAACATCGTGAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGA
GAGCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCC
AAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTACAGCGTGCTGGTGGTGGCCAAGG
TGGAGAAGGGCAAGAGCAAGAAGCTGAAATCCGTGAAGGAGCTGCTGGGCATCACCATCAT
GGAGCGGAGCAGCTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAG
GTGAAGAAGGACCTGATCATCAAGCTGCCCAAGTACAGCCTGTTCGAGCTGGAGAACGGCC
GGAAGCGGATGCTGGCCAGCGCCGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTGCCCAG
CAAGTACGTGAACTTCCTGTACCTGGCCAGCCACTACGAGAAGCTGAAGGGCAGCCCCGAG
GACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCG
AGCAGATCAGCGAGTTCAGCAAGCGGGTGATCCTGGCCGACGCCAACCTGGACAAGGTGCT
GAGCGCCTACAACAAGCACCGGGACAAGCCCATCCGGGAGCAGGCCGAGAACATCATCCAC
CTGTTCACCCTGACCAACCTGGGCGCCCCCGCCGCCTTCAAGTACTTCGACACCACCATCGA
CCGGAAGCGGTACACCAGCACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATC
ACCGGCCTGTACGAGACCCGGATCGACCTGAGCCAGCTGGGCGGCGACAGCGGCGGCAAGC
GGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGTCGGGCGGGGGTGGCT
CAGTGGACGTGCTGAGGAGAAGGAAGGACTGGAACGTGAGACTGCAAGCCTTCTTCACCAG
CGACACCGGACTGGAATACGAGGCCCCTAAGCTGTACCCCGCCATTCCCGCCGCTAGACGCA
GACCCATTAGAGTGCTCTCTCTGTTTGACGGAATCGCCACCGGCTATCTGGTGCTGAAAGAG
CTGGGCATCAAGGTGGGCAAATACGTGGCCAGCGAGGTGTGCGAGGAGTCTATCGCCGTCG
GAACCGTGAAACACGAGGGAAACATCAAATACGTGAACGACGTGAGGAACATCACCAAGA
AAAACATCGAGGAGTGGGGCCCCTTCGATCTCGTGATCGGCGGCAGCCCTTGCAACGATCTC
TCCAATGTGAACCCCGCCAGAAAGGGACTGTACGAGGGCACCGGCAGACTGTTCTTCGAGTT
CTACCATCTGCTGAACTACAGCAGACCCAAGGAGGGCGACGACAGACCCTTCTTCTGGATGT
TCGAAAACGTGGTCGCCATGAAGGTGGGCGATAAGAGGGACATCAGCAGATTTCTGGAGTG
TAACCCCGTCATGATCGATGCCATTAAGGTCAGCGCCGCCCACAGAGCTAGGTACTTCTGGG
GAAATCTGCCCGGCATGAATAGACCCGTGATCGCTTCCAAGAACGACAAGCTGGAGCTGCA
AGACTGTCTGGAGTACAATAGAATCGCTAAGCTCAAGAAGGTCCAGACCATCACCACAAAA
TCCAACAGCATCAAGCAAGGCAAGAACCAGCTGTTCCCCGTGGTCATGAACGGCAAGGAGG
ATGTGCTCTGGTGCACCGAGCTGGAGAGGATCTTTGGCTTCCCCGTGCACTATACCGATGTG
TCCAACATGGGAAGAGGCGCTAGACAGAAACTGCTGGGAAGAAGCTGGAGCGTGCCCGTGA
TTAGACACCTCTTCGCCCCTCTGAAGGATTACTTCGCTTGCGAGGGAGGTGGCGGATCGGGA
AAGCGGCCCGCCGCCACCAAGAAGGCCGGTCAGGCCAAGAAGAAGAAGGGCAGCTACCCC
TACGACGTGCCCGACTACGCCTGAGCGGCCGCTTAATTAAGCTGCCTTCTGCGGGGCTTGCC
TTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAG
TAGGAAGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (SEQ ID NO: 15) In some embodiments, an expression repressor comprises the amino acid sequence of SEQ ID NOs: 16 or 37. In some embodiments, an expression repressor described herein comprises an amino acid sequence of SEQ ID NO: 16 or 37 or a sequence with at least 80, 85, 90, 95, 99, or 100%
identity thereto, or having no more than 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10,9, 8, 7, 6, 5, 4, 3, 2, or 1 positions of difference thereto.
dCas9-DNMT3b MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNL SDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGS QILKEHPVENT QLQNEKLYLYYLQNGRDMYVD QELD IN
RLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQ
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
S KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGDSGGKRPAATKKAGQAKKKKSGGGGSVDVLRRRKDWNVRLQAFFTS
DTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKH
EGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSR
PKEGDDRPFFWMFENVVAMKVGDKRDISRFLECNPVMIDAIKVS AAHRARYFWGNLPGMNRPV
IAS KND KLELQDCLEYNRIAKLKKV QTITT KS NSIKQGKNQLFPVVMNGKEDVLWCTELERIFGF
PVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACEGGGGSGKRPAATKKAGQAKK
KKGSYPYDVPDYA (SEQ ID NO: 16) dCas9-DNMT3b without HA tag MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLI
GALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKH
ERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
DVDKLFIQLVQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLFGNLIALS
LGLTPNFKSNFDLAEDAKLQLS KDTYDDDLDNLLAQIGD QYADLFLAAKNLSDAILLSDILRVNT
EITKAPLS ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD QS KNGYAGYIDGGAS QEEFYKF
IKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS IPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGAS AQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFK
KIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAREN
QTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDIN
RLSDYDVAAIVPQSFLKDD SIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLIT Q
RKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERS SFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLAS AG
ELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLS QLGGD S GGKRPAAT KKAGQAKKKKS GGGGSVDVLRRRKDWNVRLQAFFTS
DTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKH
EGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSR
PKEGDDRPFFWMFENVVAMKVGDKRDISRFLECNPVMIDAIKVSAAHRARYFWGNLPGMNRPV
IASKNDKLELQDCLEYNRIAKLKKVQTITTKSNSIKQGKNQLFPVVMNGKEDVLWCTELERIFGF
PVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACEGGGGSGKRPAATKKAGQAKK
KKGS (SEQ ID NO: 37) Functional Characteristics An expression repression system of the present disclosure can be used to decrease expression of a target gene in a cell. In some embodiments, decreasing expression comprises decreasing the level of RNA, e.g., mRNA, encoded by the target gene. In some embodiments, decreasing expression comprises decreasing the level of a protein encoded by the target gene. In some embodiments, decreasing expression comprises both decreasing the level of mRNA and protein encoded by the target gene. In some embodiments, the expression of a target gene in a cell contacted by or comprising the expression repression system is at least 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.55x, 1.6x, 1.65x, 1.7x, 1.75x, 1.8x, 1.85x, 1.9x, 1.95x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 30x, 40x, 50x, 60x, 70x, 80x, 90x, or 100x lower than the level of expression of the target gene in a cell not contacted by or comprising the expression repression system. Expression of a target gene may be assayed by methods known to those of skill in the art, including RT-PCR, ELISA, Western blot, and the methods of Examples 2-4.
An expression repression system of the present disclosure can be used to decrease expression of a target gene in a cell for a time period. In some embodiments, the expression of a target gene in a cell contacted by or comprising the expression repression system is appreciably decreased for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6, 7, 10, or 14 days, or at least 1, 2, 3, 4, or 5 weeks, or at least 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely). Optionally, the expression of a target gene in a cell contacted by or comprising the expression repression system is appreciably decreased for no more than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 years.
In some embodiments, the expression of a target in a cell contacted by or comprising the .. expression repressor or the system is appreciably decreased for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions. An expression repressor or a system of the present disclosure can be used to methylate CpG
nucleotides in a target region. In some embodiments, the methylation persists for at least 1 days, at least 2 days, at least 5 days, at least 7 days, at least 10 days, at least 15 days, or at least 22 days post-treatment with an expression repressor or an expression repression system disclosed herein.
An expression repression system may comprise a plurality of expression repressors, where each expression repressor comprises a repressor domain with a different functionality than the repressor domain of another expression repressor. For example, an expression repression system may comprise two expression repressors, where the first expression repressor comprises a first repressor domain comprising histone deacetylase functionality and the second expression repressor comprises a second repressor domain comprising DNA methyltransferase functionality. In some embodiments, an expression repression system comprises expression repressors comprising a combination of repressor domains whose functionalities are complementary to one another with regard to inhibiting expression of a target gene, e.g., where the functionalities together enable inhibition of expression and, optionally, do not inhibit or negligibly inhibit expression when present individually. In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each expression repressor comprises a repressor domain that complements the repressor domains of each other expression repressor, e.g., each repressor domain decreases expression of a target gene.
In some embodiments, an expression repression system comprises expression repressors .. comprising a combination of repressor domains whose functionalities synergize with one another with regard to inhibiting expression of a target gene. Without wishing to be bound by theory, it is thought that epigenetic modifications to a genomic locus are cumulative, in that multiple repressive epigenetic markers (e.g., multiple different types of epigenetic markers and/or more extensive marking of a given type) individually together reduce expression more effectively than individual modifications alone (e.g., producing a greater decrease in expression and/or a longer-lasting decrease in expression). In some embodiments, an expression repression system comprises a plurality of expression repressors, wherein each expression repressor comprises a repressor domain that synergizes with the repressor domains of each other expression repressor, e.g., each repressor domain decreases expression of a target gene. In some embodiments, an expression repression system (comprising a plurality of expression repressors comprising a plurality of different repressor domains which synergize with one another) is more effective at inhibiting expression of a target gene than an individual expression repressor, e.g., and its single repressor domain. In some embodiments, an expression repression system is at least 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.55x, 1.6x, 1.65x, 1.7x, 1.75x, 1.8x, 1.85x, 1.9x, 1.95x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 30x, 40x, 50x, 60x, 70x, 80x, 90x, or 100x as effective at inhibiting expression of a target gene than an individual expression repressor. The level of an epigenetic marker may be assayed by methods known to those of skill in the art, including whole genome bisulfite sequencing, reduced representation bisulfite sequencing, bisulfite amplicon sequencing, methylation arrays, pyrosequencing, ChIP-seq, or ChIP-qPCR.
Combinations of Repressors In some embodiments, an expression repression system comprises a first expression repressor comprising a first repressor domain and a second expression repressor comprising a second repressor domain wherein the first repressor domain and second repressor domain are different from one another. In some embodiments, the first repressor domain is or comprises a first epigenetic modifying moiety (e.g., that increases or decreases a first epigenetic marker) or functional fragment thereof and the second repressor domain is or comprises a second epigenetic modifying moiety (e.g., that increases or decreases a second epigenetic marker) or functional fragment thereof. In some embodiments, the first repressor domain is or comprises a histone methyltransferase or functional fragment thereof and the second repressor domain is or comprises a DNA methyltransferase or functional fragment thereof. In some embodiments, the first repressor domain is or comprises a histone deacetylase or functional fragment thereof and the second repressor domain is or comprises a DNA
methyltransferase or functional fragment thereof. In some embodiments, the first repressor domain is or comprises a histone deacetylase or functional fragment thereof and the second repressor domain is or comprises a histone methyltransferase or functional fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB (e.g., a KRAB domain), a SET domain (e.g., the SET domain of SETDB1 EZH2, G9A, or SUV39H1), histone demethylase LSD1, FOG1 (e.g., the N-terminal residues of FOG1), HDAC8, MQ1, DNMT1, DNMT3a/31, or KAP1, or a functional fragment of any thereof, and the second repressor domain is or comprises KRAB (e.g., a KRAB domain), a SET domain (e.g., the SET domain of SETDB1 EZH2, G9A, or SUV39H1), histone demethylase LSD1, FOG1 (e.g., the N-terminal residues of FOG1), DNMT3A (e.g., human DNMT3A), DNMT3B, DNMT3L, DNMT3A/3L complex, or bacterial MQ1, or a functional fragment of any thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises DNMT3A or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises DNMT3B or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises DNMT3L or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KRAB or a functional variant or fragment thereof, and the second repressor domain is or comprises DNMT3A/3L
complex or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises a SET domain or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises LSD1 or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises FOG1 or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant or fragment thereof.
In some embodiments, the first repressor domain is or comprises KAP1 or a functional variant or fragment thereof, and the second repressor domain is or comprises bacterial MQ1 or a functional variant .. or fragment thereof.
In some embodiments, an expression repression system comprises a first expression repressor, wherein the first expression repressor is an expression repressor chosen from dCas9-MQ1, dCas9-DNMT1, dCas9-DNMT3a/31, G9A-dCas9, dCas9-HDAC8, dCas9-LSD1, EZH2-dCas9, EZH2-dCas9-KRAB, G9a-dCas9-KRAB, and Fogl-dCas9-Fogl, and a second expression repressor, wherein the second expression repressor is an expression repressor chosen from dCas9-MQ1, dCas9-DNMT1, dCas9-DNMT3a/31, G9A-dCas9, dCas9-HDAC8, dCas9-LSD1, EZH2-dCas9, EZH2-dCas9-KRAB, G9a-dCas9-KRAB, and Fogl-dCas9-Fogl.
In some embodiments, the expression repression system is encoded by a first nucleic acid encoding the first expression repressor, wherein expression is driven by a first promoter or IRES, and a second nucleic acid encoding the second expression repressor, wherein expression is driven by a second promoter or IRES.
Target Sites Expression repression systems disclosed herein are useful for modulating, e.g., decreasing, expression of a target gene in cell, e.g., in a subject or patient. A target gene may be any gene known to those of skill in the art. In some embodiments, a target gene is associated with a disease or condition in a subject, e.g., a mammal, e.g., a human, bovine, horse, sheep, chicken, rat, mouse, cat, or dog. A target gene may include coding sequences, e.g., exons, and/or non-coding sequences, e.g., introns, 3'UTR, or 5'UTR. In some embodiments, a target gene is operably linked to a transcription control element.
A DNA-targeting moiety suitable for use in an expression repressor of an expression repression system described herein may bind, e.g., specifically bind, to any site within a target gene, transcription control element operably linked to a target gene, or anchor sequence (e.g., an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene (e.g., an anchor sequence-mediated conjunction is operably linked to a target gene if disruption of the conjunction alters expression of the target gene)).
In some embodiments, a DNA-targeting moiety binds to a target gene. In some embodiments, a DNA-targeting moiety binds to a site within an exon of a target gene. In some embodiments, a DNA-targeting moiety binds to a site within an intron of a target gene. In some embodiments, a DNA-targeting moiety binds to a site at the boundary of an exon and an intron, e.g., a splice site, of a target gene. In some embodiments, a DNA-targeting moiety binds to a site within the 5'UTR of a target gene. In some embodiments, a DNA-targeting moiety binds to a site within the 3'UTR of a target gene. Target genes include, but are not limited to, the gene encoding13-2-microglobulin, the gene encoding MYC, the gene encoding HSPA1B, the gene encoding GATA1, the gene encoding APOB, the gene encoding FOXP3, the gene encoding CXCL1, the gene encoding CXCL2, the gene encoding CXCL3, the gene encoding CXCL4, the gene encoding CXCL5, the gene encoding CXCL6, the gene encoding CXCL7, and the gene encoding CXCL8.
In some embodiments, a DNA-targeting moiety binds to a transcription control element operably linked to a target gene, e.g., a promoter or enhancer. In some embodiments, a DNA-targeting moiety binds to a portion of or a site within a promoter operably linked to a target gene. In some embodiments, a DNA-targeting moiety binds to the transcription start site of a target gene.
In some embodiments, a DNA-targeting moiety binds to a portion of or a site within an enhancer operably linked to a target gene. A
promoter is, typically, a sequence element that initiates transcription of an associated gene. Promoters are typically near the 5' end of a gene, not far from its transcription start site. As those of ordinary skill are aware, transcription of protein-coding genes in eukaryotic cells is typically initiated by binding of general transcription factors (e.g., TFIID, TFIIE, TFIIH, etc.) and Mediator to core promoter sequences as a preinitiation complex that directs RNA polymerase II to the transcription start site, and in many instances remains bound to the core promoter sequences even after RNA polymerase escapes and elongation of the primary transcript is initiated. In some embodiments, a promoter includes a sequence element such as TATA, Inr, DPE, or BRE, but those skilled in the art are well aware that such sequences are not necessarily required to define a promoter. Those skilled in the art are familiar with a variety of positive .. (e.g., enhancers) or negative (e.g., repressors or silencers) transcription control elements that are associated with genes. In some embodiments, a transcription control element is a transcription factor binding site. Typically, when a cognate regulatory protein is bound to such a transcription control element, transcription from the associated gene(s) is altered (e.g., increased or decreased).
In some embodiments, a DNA-targeting moiety binds to an anchor sequence, e.g., an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene (e.g., an anchor sequence-mediated conjunction is operably linked to a target gene if disruption of the conjunction alters expression of the target gene). In some embodiments, a DNA-targeting moiety binds to or proximal to an anchor sequence of an anchor sequence mediated conjunction (ASMC) comprising a target plurality of genes, e.g., human CXCL1-8. In general, an anchor sequence is a genomic sequence element to which a genomic complex component binds specifically. In some embodiments, binding of a genomic complex component to an anchor sequence nucleates complex formation, e.g., anchor sequence-mediated conjunction formation. Each anchor sequence-mediated conjunction comprises one or more anchor sequences, e.g., a plurality. In some embodiments, an anchor sequence-mediated conjunction can be disrupted to alter, e.g., inhibit, expression of a target gene. Such disruptions may modulate gene expression by, e.g., changing topological structure of DNA, e.g., by modulating the ability of a target gene to interact with a transcription control element (e.g., enhancing and silencing/repressive sequences).
A DNA-targeting moiety suitable for use in an expression repressor of an expression repression system described herein may bind, e.g., specifically bind, to a site that is proximal to a target gene (e.g., an exon, intron, or splice site within the target gene), proximal to a transcription control element operably linked to the target gene, or proximal to an anchor sequence, e.g., an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene (e.g., an anchor sequence-mediated conjunction is operably linked to a target gene if disruption of the conjunction alters expression of the target gene). As used herein, proximal refers to a closeness of two sites, e.g., nucleic acid sites, such that binding of an expression repressor at the first site and/or modification of the first site by an expression repressor will produce the same or substantially the same effect as binding and/or modification of the other site. For example, a DNA-targeting moiety may bind to a first site that is proximal to an enhancer (the second site), and the repressor domain associated with said DNA-targeting moiety may epigenetically modify the first site such that the enhancer's effect on expression of a target gene is modified, substantially the same as if the second site (the enhancer sequence) had been bound and/or modified. In some embodiments, a site proximal to a target gene (e.g., an exon, intron, or splice site within the target gene), proximal to a transcription control element operably linked to the target gene, or proximal to an anchor sequence is less than 5000, 4000, 3000, 2000, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50, or 25 base pairs from the target gene (e.g., an exon, intron, or splice site within the target gene), transcription control element, or anchor sequence (and optionally at least 20, 25, 50, 100, 200, or 300 base pairs from the target gene (e.g., an exon, intron, or splice site within the target gene), transcription control element, or anchor sequence).
A DNA-targeting moiety suitable for use in an expression repressor of an expression repression system described herein may bind, e.g., specifically bind, to a site comprising at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides or base pairs (and optionally no more 50, 49, 48, 47, 46, 45, 44, 43, 42, 41, 40, 39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, or 10 nucleotides or base pairs). In some embodiments, a DNA-targeting moiety binds to a site comprising 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides or base pairs.
Expression repression systems of the present disclosure may comprise two or more expression repressors. In some embodiments, the expression repressors of an expression repression system each comprise a different DNA-targeting moiety.
In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds a target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site), and second expression repressor comprising a DNA-targeting moiety that binds the target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site). In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds a target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site), and second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to the target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site). In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to the target gene or associated with an anchor sequence-mediated conjunction operably linked to the target gene.
In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to a first site, e.g., in a promoter operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a second site, e.g., in the promoter operably linked to a target gene. The first site and second site may be different and non-overlapping sites, e.g., the first site and second site do not share any sequence in common. The first site and second site may be different but overlapping sites, e.g., the first site and second site comprise different sequences but share some sequence in common.
In some embodiments, a DNA-targeting moiety binds to a sequence selected from SEQ ID NOs:
1-21 or a sequence with no more than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 alteration relative thereto.
ACGGCGGGCCACCAAGGAGA (SEQ ID NO: 6) CAGCGCCGCGCCTTTGGGAC (SEQ ID NO: 7) CCCTTTCGGCGGGGAGCAGGG (SEQ ID NO: 8) GCTGCGCTGGGGGAGCCAGAG (SEQ ID NO: 9) AGCGCCCCTCCACGCGTTCAC (SEQ ID NO: 10) In some embodiments, the first DNA-targeting moiety binds to a sequence comprising any one of SEQ ID NOS: 1-21 and the second DNA-targeting moiety binds to a sequence comprising any one of SEQ ID NOS: 1-21, wherein the first and the second DNA-targeting moiety binds to the same sequence.
In some embodiments, the first DNA-targeting moiety binds to a sequence comprising any one of SEQ ID
NOS: 1-21 and the second DNA-targeting moiety binds to a sequence comprising any one of SEQ ID
NOS: 1-21 wherein the first and the second targeting moiety binds to different sequences.
In some embodiments, an expression repressor binds a genomic locus having a sequence set forth herein, e.g., any one of SEQ ID NOS: 1-21. It is understood that, in many cases, the genomic locus being bound comprises double stranded DNA, and this locus can be described by giving the sequence of its sense strand or its antisense strand. Thus, a gRNA having a given spacer sequence may cause expression repressor to bind to a particular genomic locus, wherein one strand of the genomic locus has a sequence similar or identical to the spacer sequence, and the other strand of the genomic locus has the complementary sequence. Typically, gRNA binding to the genomic locus will involve some unwinding of the genomic locus and pairing of the gRNA spacer with the strand to which the spacer is complementary.
In some embodiments, an anchor sequence comprises a nucleating polypeptide binding motif, e.g., a CTCF-binding motif:
N(T/C/G)N(G/A/T)CC(A/T/G)(C/G)(C/T/A)AG(G/A)(G/T)GG(C/A/T)(G/A)(C/G)(C/T/A)(G/A
/C) (SEQ ID NO: 81), where N is any nucleotide.
A CTCF-binding motif may also be in an opposite orientation, e.g., (G/A/C)(C/T/A)(C/G)(G/A)(C/A/T)GG(G/T)(G/A)GA(C/T/A)(C/G)(A/T/G)CC(G/A/T)N(T/C/
G)N
(SEQ ID NO: 82). where N is any nucleotide In some embodiments, an anchor sequence comprises SEQ ID NO: 81 or SEQ ID NO:
82 or a sequence at least 75%, at least 80%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identical to either SEQ ID NO: 81 or SEQ ID NO: 82.
In some embodiments, an anchor sequence comprises a nucleating polypeptide binding motif, e.g., a YY1-binding motif: CCGCCATNTT (SEQ ID NO: 83), where N is any nucleotide.
A YY1-binding motif may also be in an opposite orientation, e.g., AANATGGCGG
(SEQ ID
NO: 84), where N is any nucleotide.
A targeting moiety suitable for use in an expression repressor of an expression repression system described herein may bind, e.g., specifically bind, to a site comprising at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides or base pairs (and optionally no more 50, 49, 48, 47, 46, 45, 44, 43, 42, 41, 40, 39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, or 10 nucleotides or base pairs). In some embodiments, a DNA-targeting moiety binds to a site comprising 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides or base pairs.
Expression repression systems of the present disclosure may comprise two or more expression repressors. In some embodiments, the expression repressors of an expression repression system each comprise a different targeting moiety.
In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds a target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site), and second expression repressor comprising a DNA-targeting moiety that binds the target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site). In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds a target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site), and second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene, e or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to a transcription control element (e.g., promoter or enhancer) operably linked to the target gene,. In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene, or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to the target gene, e.g., an exon, intron, or exon intron boundary (e.g., splice site). In some embodiments, an expression repression system comprises a first expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to a target gene, or associated with an anchor sequence-mediated conjunction operably linked to a target gene, and a second expression repressor comprising a DNA-targeting moiety that binds to an anchor sequence proximal to the target gene, or associated with an anchor sequence-mediated conjunction operably linked to the target gene.
Other Compositions Nucleic acids and Vectors The present disclosure is further directed, in part, to nucleic acids encoding expression repressors or expression repression systems described herein. In some embodiments, an expression repression system may be provided via a composition comprising a nucleic acid encoding the expression repression system, e.g., expression repressor(s) of the expression repression system, wherein the nucleic acid is associated with sufficient other sequences to achieve expression of the expression repression system, e.g., expression repressor(s) of the expression repression system, in a system of interest (e.g., in a particular cell, tissue, organism, etc.).
In some particular embodiments, the present disclosure provides compositions of nucleic acids that encode an expression repression system, one or more expression repressors, or polypeptide portion thereof. In some such embodiments, provided nucleic acids may be or include DNA, RNA, or any other nucleic acid moiety or entity as described herein, and may be prepared by any technology described herein or otherwise available in the art (e.g., synthesis, cloning, amplification, in vitro or in vivo transcription, etc.). In some embodiments, provided nucleic acids that encode an expression repression system, one or more expression repressors, or polypeptide portion thereof may be operationally associated with one or more replication, integration, and/or expression signals appropriate and/or sufficient to achieve integration, replication, and/or expression of the provided nucleic acid in a system of interest (e.g., in a particular cell, tissue, organism, etc.). In some embodiments, a composition for delivering an expression repression system described herein is or comprises a vector, e.g., a viral vector, comprising .. one or more nucleic acids encoding one or more components of an expression repression system, e.g., expression repressor(s) of the expression repression system as described herein.
In some embodiments, a composition for delivering an expression repressor or an expression repression system described herein is or comprises RNA, e.g., mRNA, comprising one or more nucleic acids encoding one or more components of an expression repressor or an expression repression system, e.g., expression repressor(s) of the expression repression system as described herein.
Nucleic acids as described herein or nucleic acids encoding a protein described herein, may be incorporated into a vector. Vectors, including those derived from retroviruses such as lentivirus, are .. suitable tools to achieve long-term gene transfer since they allow long-term, stable integration of a transgene and its propagation in daughter cells. Examples of vectors include expression vectors, replication vectors, probe generation vectors, and sequencing vectors. An expression vector may be provided to a cell in the form of a viral vector. Viral vector technology is well known in the art and described in a variety of virology and molecular biology manuals. Viruses, which are useful as vectors .. include, but are not limited to, retroviruses, adenoviruses, adeno-associated viruses, herpes viruses, and lentiviruses. In general, a suitable vector contains an origin of replication functional in at least one organism, a promoter sequence, convenient restriction endonuclease sites, and one or more selectable markers.
Expression of natural or synthetic nucleic acids is typically achieved by operably linking a .. nucleic acid encoding the gene of interest to a promoter and incorporating the construct into an expression vector. Vectors can be suitable for replication and integration in eukaryotes.
Typical cloning vectors contain transcription and translation terminators, initiation sequences, and promoters useful for expression of the desired nucleic acid sequence.
Additional promoter elements, e.g., enhancing sequences, may regulate frequency of .. transcriptional initiation. Typically, these sequences are located in a region 30-110 bp upstream of a transcription start site, although a number of promoters have recently been shown to contain functional elements downstream of transcription start sites as well. Spacing between promoter elements frequently is flexible, so that promoter function is preserved when elements are inverted or moved relative to one another. In a thymidine kinase (tk) promoter, spacing between promoter elements can be increased to 50 .. bp apart before activity begins to decline. Depending on the promoter, it appears that individual elements can function either cooperatively or independently to activate transcription.
One example of a suitable promoter is the immediate early cytomegalovirus (CMV) promoter sequence. This promoter sequence is a strong constitutive promoter sequence capable of driving high levels of expression of any polynucleotide sequence operatively linked thereto. In some embodiments of .. a suitable promoter is Elongation Growth Factor-1a (EF-1a). However, other constitutive promoter sequences may also be used, including, but not limited to the simian virus 40 (5V40) early promoter, mouse mammary tumor virus (MMTV), human immunodeficiency virus (HIV) long terminal repeat (LTR) promoter, MoMuLV promoter, an avian leukemia virus promoter, an Epstein-Barr virus immediate early promoter, a Rous sarcoma virus promoter, as well as human gene promoters such as, but not limited to, an actin promoter, a myosin promoter, a hemoglobin promoter, and a creatine kinase promoter.
The present disclosure should not interpreted to be limited to use of any particular promoter or category of promoters (e.g. constitutive promoters). For example, in some embodiments, inducible promoters are contemplated as part of the present disclosure. In some embodiments, use of an inducible promoter provides a molecular switch capable of turning on expression of a polynucleotide sequence to which it is operatively linked, when such expression is desired. In some embodiments, use of an inducible promoter provides a molecular switch capable of turning off expression when expression is not desired. Examples of inducible promoters include, but are not limited to a metallothionine promoter, a glucocorticoid promoter, a progesterone promoter, and a tetracycline promoter.
In some embodiments, an expression vector to be introduced can also contain either a selectable marker gene or a reporter gene or both to facilitate identification and selection of expressing cells from the population of cells sought to be transfected or infected through viral vectors. In some aspects, a selectable marker may be carried on a separate piece of DNA and used in a co-transfection procedure.
Both selectable markers and reporter genes may be flanked with appropriate transcriptional control sequences to enable expression in the host cells. Useful selectable markers may include, for example, antibiotic-resistance genes, such as neo, etc.
In some embodiments, reporter genes may be used for identifying potentially transfected cells and/or for evaluating the functionality of transcriptional control sequences.
In general, a reporter gene is a gene that is not present in or expressed by a recipient source (of a reporter gene) and that encodes a polypeptide whose expression is manifested by some easily detectable property, e.g., enzymatic activity or visualizable fluorescence. Expression of a reporter gene is assayed at a suitable time after the DNA
has been introduced into the recipient cells. Suitable reporter genes may include genes encoding luciferase, beta-galactosidase, chloramphenicol acetyl transferase, secreted alkaline phosphatase, or the green fluorescent protein gene (e.g., Ui-Tei et al., 2000 FEBS Letters 479: 79-82). Suitable expression systems are well known and may be prepared using known techniques or obtained commercially. In general, a construct with a minimal 5' flanking region that shows highest level of expression of reporter gene is identified as a promoter. Such promoter regions may be linked to a reporter gene and used to evaluate agents for ability to modulate promoter-driven transcription.
Cells The present disclosure is further directed, in part, to cells comprising an expression repressor or an expression repression system described herein. Any cell, e.g., cell line, e.g., a cell line suitable for expression of a recombinant polypeptide, known to one of skill in the art is suitable to comprise an expression repressor or an expression repression system described herein. In some embodiments, a cell, e.g., cell line, may be used to express an expression repressor or an expression repression system, e.g., expression repressor(s), described herein. In some embodiments, a cell, e.g., cell line, may be used to express or amplify a nucleic acid, e.g., a vector, encoding an expression repressor or an expression repression system, e.g., expression repressor(s), described herein. In some embodiments, a cell comprises a nucleic acid encoding an expression repressor or an expression repression system, e.g., expression repressor(s), described herein.
In some embodiments, a cell comprises a first nucleic acid encoding a first component of an expression repressor or an expression repression system, e.g., a first expression repressor, and a second nucleic acid encoding a second component of the expression repression system, e.g., a second expression repressor. In some embodiments, wherein a cell comprises nucleic acid encoding an expression repression system comprising two or more expression repressors, the sequences encoding each expression repressor are disposed on separate nucleic acid molecules, e.g., on different vectors, e.g., a first vector encoding a first expression repressor and a second vector encoding a second expression repressor. In some embodiments, the sequences encoding each expression repressor are disposed on the same nucleic acid molecule, e.g., on the same vector. In some embodiments, some or all of the nucleic acid encoding the expression repression system is integrated into the genomic DNA of the cell.
In some embodiments, the nucleic acid encoding a first expression repressor of an expression repression system is integrated into the genomic DNA of a cell, and the nucleic acid encoding a second expression repressor of an expression repression system is not integrated into the genomic DNA of a cell (e.g., is situated on a vector). In some embodiments, the nucleic acid(s) encoding a first and a second expression repressor of an expression repression system are integrated into the genomic DNA of a cell, e.g., at the same (e.g., adjacent or colocalized) or different sites in the genomic DNA.
Examples of cells that may comprise and/or express an expression repression system or expression repressor described herein include, but are not limited to, hepatocytes, neuronal cells, endothelial cells, myocytes, and lymphocytes.
The present disclosure is further directed, in part, to a cell made by a method or process described herein. In some embodiments, the disclosure provides a cell produced by:
providing an expression repression system described herein, providing the cell, and contacting the cell with the expression repression system (or a nucleic acid encoding the expression repression system, or a composition comprising said expression repression system or nucleic acid). Without wishing to be bound by theory, a cell contacted with an expression repression system described herein may exhibit: a decrease in expression of a target gene and/or a modification of epigenetic markers associated with the target gene, a transcription control element operably linked to the target gene, or an anchor sequence proximal to the target gene or associated with an anchor sequence-mediated conjunction operably linked to the target gene compared to a similar cell that has not been contacted by the expression repression system. In some embodiments, a cell exhibiting said decrease in expression of a target gene and/or modification of epigenetic markers does not comprise the expression repression system. The decrease in expression and/or modification of epigenetic markers may persist, e.g., for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6,7, 10, or 14 days, or at least 1,2, 3, 4, or 5 weeks, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely) after contact with the expression repression system. In some embodiments, a cell previously contacted by an expression repression system retains the decrease in expression and/or modification of epigenetic markers after the expression repression system is no longer present in the cell, e.g., for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6, 7, 10, or 14 days, or at least 1, 2, 3, 4, or 5 weeks, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely) after the expression repression system is no longer present in the cell.
Methods of Making Expression Repression Systems and/or Expression Repressors In some embodiments, an expression repressor comprises or is a protein and may thus be produced by methods of making proteins. In some embodiments, an expression repression system, e.g., the expression repressor(s) of an expression repression system, comprise one or more proteins and may thus be produced by methods of making proteins. As will be appreciated by one of skill, methods of making proteins or polypeptides (which may be included in modulating agents as described herein) are routine in the art. See, in general, Smales & James (Eds.), Therapeutic Proteins: Methods and Protocols (Methods in Molecular Biology), Humana Press (2005); and Crommelin, Sindelar &
Meibohm (Eds.), Pharmaceutical Biotechnology: Fundamentals and Applications, Springer (2013).
A protein or polypeptide of compositions of the present disclosure can be biochemically synthesized by employing standard solid phase techniques. Such methods include exclusive solid phase synthesis, partial solid phase synthesis methods, fragment condensation, classical solution synthesis.
These methods can be used when a peptide is relatively short (e.g., 10 kDa) and/or when it cannot be produced by recombinant techniques (i.e., not encoded by a nucleic acid sequence) and therefore involves different chemistry.
Solid phase synthesis procedures are well known in the art and further described by John Morrow Stewart and Janis Dillaha Young, Solid Phase Peptide Syntheses, 2nd Ed., Pierce Chemical Company, 1984; and Coin, I., et al., Nature Protocols, 2:3247-3256, 2007.
For longer peptides, recombinant methods may be used. Methods of making a recombinant therapeutic polypeptide are routine in the art. See, in general, Smales &
James (Eds.), Therapeutic Proteins: Methods and Protocols (Methods in Molecular Biology), Humana Press (2005); and Crommelin, .. Sindelar & Meibohm (Eds.), Pharmaceutical Biotechnology: Fundamentals and Applications, Springer (2013).
Exemplary methods for producing a therapeutic pharmaceutical protein or polypeptide involve expression in mammalian cells, although recombinant proteins can also be produced using insect cells, yeast, bacteria, or other cells under control of appropriate promoters.
Mammalian expression vectors may comprise non transcribed elements such as an origin of replication, a suitable promoter, and other 5' or 3' flanking non transcribed sequences, and 5' or 3' non-translated sequences such as necessary ribosome binding sites, a polyadenylation site, splice donor and acceptor sites, and termination sequences. DNA
sequences derived from the 5V40 viral genome, for example, 5V40 origin, early promoter, splice, and polyadenylation sites may be used to provide other genetic elements required for expression of a heterologous DNA sequence. Appropriate cloning and expression vectors for use with bacterial, fungal, yeast, and mammalian cellular hosts are described in Green & Sambrook, Molecular Cloning: A
Laboratory Manual (Fourth Edition), Cold Spring Harbor Laboratory Press (2012).
In cases where large amounts of the protein or polypeptide are desired, it can be generated using techniques such as described by Brian Bray, Nature Reviews Drug Discovery, 2:587-593, 2003; and Weissbach & Weissbach, 1988, Methods for Plant Molecular Biology, Academic Press, NY, Section VIII, pp 421-463.
Various mammalian cell culture systems can be employed to express and manufacture recombinant protein. Examples of mammalian expression systems include CHO
cells, COS cells, HeLA
and BHK cell lines. Processes of host cell culture for production of protein therapeutics are described in Zhou and Kantardjieff (Eds.), Mammalian Cell Cultures for Biologics Manufacturing (Advances in Biochemical Engineering/Biotechnology), Springer (2014). Compositions described herein may include a vector, such as a viral vector, e.g., a lentiviral vector, encoding a recombinant protein. In some embodiments, a vector, e.g., a viral vector, may comprise a nucleic acid encoding a recombinant protein.
Compositions described herein may include a lipid nanoparticle encapsulating a vector, such as a viral vector, e.g., a lentiviral vector, encoding a recombinant protein. In some embodiments, a lipid nanoparticle encapsulating a vector, e.g., a viral vector, may comprise a nucleic acid encoding a recombinant protein.
Proteins comprise one or more amino acids. Amino acids include any compound and/or substance that can be incorporated into a polypeptide chain, e.g., through formation of one or more peptide bonds.
In some embodiments, an amino acid has the general structure H2N¨C(H)(R)¨COOH.
In some embodiments, an amino acid is a naturally occurring amino acid. In some embodiments, an amino acid is a non-natural amino acid; in some embodiments, an amino acid is a D-amino acid; in some embodiments, an amino acid is an L-amino acid. "Standard amino acid" refers to any of the twenty standard L-amino acids commonly found in naturally occurring peptides. "Nonstandard amino acid"
refers to any amino acid, other than the standard amino acids, regardless of whether it is prepared synthetically or obtained from a natural source. In some embodiments, an amino acid, including a carboxy-and/or amino-terminal amino acid in a polypeptide, can contain a structural modification as compared with the general structure above. For example, in some embodiments, an amino acid may be modified by methylation, amidation, acetylation, pegylation, glycosylation, phosphorylation, and/or substitution (e.g., of the amino group, the carboxylic acid group, one or more protons, and/or the hydroxyl group) as compared with the general structure. In some embodiments, such modification may, for example, alter the circulating half-life of a polypeptide containing the modified amino acid as compared with one containing an otherwise identical unmodified amino acid. In some embodiments, such modification does not significantly alter a relevant activity of a polypeptide containing the modified amino acid, as compared with one containing an otherwise identical unmodified amino acid. As will be clear from context, in some embodiments, the term "amino acid" may be used to refer to a free amino acid; in some embodiments it may be used to refer to an amino acid residue of a polypeptide.
Purification of protein therapeutics is described in Franks, Protein Biotechnology: Isolation, Characterization, and Stabilization, Humana Press (2013); and in Cutler, Protein Purification Protocols (Methods in Molecular Biology), Humana Press (2010). Formulation of protein therapeutics is described in Meyer (Ed.), Therapeutic Protein Drug Products: Practical Approaches to formulation in the Laboratory, Manufacturing, and the Clinic, Woodhead Publishing Series (2012).
Pharmaceutical Compositions, Formulation, Delivery, and Administration The present disclosure is further directed, in part, to pharmaceutical compositions comprising an expression repressor or an expression repression system, e.g., expression repressor(s), described herein, to pharmaceutical compositions comprising nucleic acids encoding the expression repressor or the expression repression system, e.g., expression repressor(s), described herein, and/or to and/or compositions that deliver an expression repressor or an expression repression system, e.g., expression repressor(s), described herein to a cell, tissue, organ, and/or subject.
As used herein, the term "pharmaceutical composition" refers to an active agent (e.g., an expression repressor or nucleic acids of the expression receptor, e.g., an expression repression system, e.g., expression repressor(s) of an expression repression system, or nucleic acid encoding the same), formulated together with one or more pharmaceutically acceptable carriers (e.g., pharmaceutically acceptable carriers known to those of skill in the art). In some embodiments, active agent is present in unit dose amount appropriate for administration in a therapeutic regimen that shows a statistically significant probability of achieving a predetermined therapeutic effect when administered to a relevant population. In some embodiments, a pharmaceutical composition comprising an expression repressor of the present disclosure comprises an expression repressor or nucleic acid(s) encoding the same. In some embodiments, a pharmaceutical composition comprising an expression repression system of the present disclosure comprises or each of the expression repressors of the expression repression system or nucleic acid(s) encoding the same (e.g., if an expression repression system comprises a first expression repressor and a second expression repressor, the pharmaceutical composition comprises the first and second expression repressor). In some embodiments, a pharmaceutical composition comprises less than all of the expression repressors of an expression repression system comprising a plurality of expression repressors.
For example, an expression repression system may comprise a first expression repressor and a second expression repressor, and a first pharmaceutical composition may comprise the first expression repressor or nucleic acid encoding the same and a second pharmaceutical composition may comprise the second expression repressor or nucleic acid encoding the same.
In some embodiments, pharmaceutical compositions may be specially formulated for administration in solid or liquid form, including those adapted for the following: oral administration, for example, drenches (aqueous or non-aqueous solutions or suspensions), tablets, e.g., those targeted for buccal, sublingual, and systemic absorption, boluses, powders, granules, pastes for application to the tongue; parenteral administration, for example, by subcutaneous, intramuscular, intravenous or epidural injection as, for example, a sterile solution or suspension, or sustained-release formulation; topical application, for example, as a cream, ointment, or a controlled-release patch or spray applied to the skin, lungs, or oral cavity; intravaginally or intrarectally, for example, as a pessary, cream, or foam;
sublingually; ocularly; transdermally; or nasally, pulmonary, and/or to other mucosal surfaces.
As used herein, the term "pharmaceutically acceptable" refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
As used herein, the term "pharmaceutically acceptable carrier" means a pharmaceutically acceptable material, composition, or vehicle, such as a liquid or solid filler, diluent, excipient, or solvent encapsulating material, involved in carrying or transporting the subject compound from one organ, or portion of the body, to another organ, or portion of the body. Each carrier must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not injurious to the patient.
In some embodiments, for example, materials which can serve as pharmaceutically-acceptable carriers include: sugars, such as lactose, glucose and sucrose; starches, such as corn starch and potato starch; cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar;
buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water;
isotonic saline;
Ringer's solution; ethyl alcohol; pH buffered solutions; polyesters, polycarbonates and/or polyanhydrides;
and other non-toxic compatible substances employed in pharmaceutical formulations.
As used herein, the term "pharmaceutically acceptable salt", refers to salts of such compounds that are appropriate for use in pharmaceutical contexts, i.e., salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response, and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge, et al.
describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 66: 1-19 (1977). In some embodiments, pharmaceutically acceptable salts include, but are not limited to, nontoxic acid addition salts, which are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, maleic acid, tartaric acid, citric acid, succinic acid, or malonic acid or by using other methods used in the art such as ion exchange. In some embodiments, pharmaceutically acceptable salts include, but are not limited to, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. In some embodiments, pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl having from 1 to 6 carbon atoms, sulfonate, and aryl sulfonate.
In various embodiments, the present disclosure provides pharmaceutical compositions described herein with a pharmaceutically acceptable excipient. Pharmaceutically acceptable excipient includes an excipient that is useful in preparing a pharmaceutical composition that is generally safe, non-toxic, and desirable, and includes excipients that are acceptable for veterinary use as well as for human pharmaceutical use. Such excipients may be solid, liquid, semisolid, or, in the case of an aerosol composition, gaseous.
Pharmaceutical preparations may be made following conventional techniques of pharmacy involving milling, mixing, granulation, and compressing, when necessary, for tablet forms; or milling, mixing, and filling for hard gelatin capsule forms. When a liquid carrier is used, a preparation can be in the form of a syrup, elixir, emulsion or an aqueous or non-aqueous solution or suspension. Such a liquid formulation may be administered directly per os.
In some embodiments, pharmaceutical compositions may be formulated for delivery to a cell and/or to a subject via any route of administration. Modes of administration to a subject may include injection, infusion, inhalation, intranasal, intraocular, topical delivery, intercannular delivery, or ingestion.
Injection includes, without limitation, intravenous, intramuscular, intra-arterial, intrathecal, intraventricular, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, sub capsular, subarachnoid, intraspinal, intracerebrospinal, and intrasternal injection and infusion. In some embodiments, administration includes aerosol inhalation, e.g., with nebulization. In some embodiments, administration is systemic (e.g., oral, rectal, nasal, sublingual, buccal, or parenteral), enteral (e.g., system-wide effect, but delivered through the gastrointestinal tract), or local (e.g., local application on the skin, intravitreal injection). In some embodiments, one or more compositions is administered systemically. In some embodiments, administration is non-parenteral and a therapeutic is a parenteral therapeutic. In some embodiments, administration may be bronchial (e.g., by bronchial instillation), buccal, dermal (which may be or comprise, for example, one or more of topical to the dermis, intradermal, interdermal, transdermal, etc.), enteral, intra-arterial, intradermal, intragastric, intramedullary, intramuscular, intranasal, intraperitoneal, intrathecal, intravenous, intraventricular, within a specific organ (e. g. intrahepatic), mucosal, nasal, oral, rectal, subcutaneous, sublingual, topical, tracheal (e.g., by intratracheal instillation), vaginal, vitreal, etc. In some embodiments, administration may be a single dose. In some embodiments, administration may involve dosing that is intermittent (e.g., a plurality of doses separated in time) and/or periodic (e.g., individual doses separated by a common period of time) dosing. In some embodiments, administration may involve continuous dosing (e.g., perfusion) for at least a selected period of time.
Pharmaceutical compositions according to the present disclosure may be delivered in a therapeutically effective amount. A precise therapeutically effective amount is an amount of a composition that will yield the most effective results in terms of efficacy of treatment in a given subject. This amount will vary depending upon a variety of factors, including but not limited to characteristics of a therapeutic compound (including activity, pharmacokinetics, pharmacodynamics, and bioavailability), physiological condition of a subject (including age, sex, disease type and stage, general physical condition, responsiveness to a given dosage, and type of medication), nature of a pharmaceutically acceptable carrier or carriers in a formulation, and/or route of administration.
In some aspects, the present disclosure provides methods of delivering a therapeutic comprising administering a composition as described herein to a subject, wherein a genomic complex modulating agent is a therapeutic and/or wherein delivery of a therapeutic causes changes in gene expression relative to gene expression in absence of a therapeutic.
Methods as provided in various embodiments herein may be utilized in any some aspects delineated herein. In some embodiments, one or more compositions is/are targeted to specific cells, or one or more specific tissues.
For example, in some embodiments one or more compositions is/are targeted to epithelial, connective, muscular, and/or nervous tissue or cells. In some embodiments a composition is targeted to a cell or tissue of a particular organ system, e.g., cardiovascular system (heart, vasculature); digestive system (esophagus, stomach, liver, gallbladder, pancreas, intestines, colon, rectum and anus); endocrine system (hypothalamus, pituitary gland, pineal body or pineal gland, thyroid, parathyroids, adrenal glands); excretory system (kidneys, ureters, bladder); lymphatic system (lymph, lymph nodes, lymph vessels, tonsils, adenoids, thymus, spleen); integumentary system (skin, hair, nails); muscular system (e.g., skeletal muscle); nervous system (brain, spinal cord, nerves);
reproductive system (ovaries, uterus, mammary glands, testes, vas deferens, seminal vesicles, prostate); respiratory system (pharynx, larynx, trachea, bronchi, lungs, diaphragm); skeletal system (bone, cartilage); and/or combinations thereof.
In some embodiments, a composition of the present disclosure crosses a blood-brain-barrier, a placental membrane, or a blood-testis barrier.
In some embodiments, a pharmaceutical composition as provided herein is administered systemically.
In some embodiments, administration is non-parenteral and a therapeutic is a parenteral therapeutic.
In some embodiments, a pharmaceutical composition of the present disclosure has improved PK/PD, e.g., increased pharmacokinetics or pharmacodynamics, such as improved targeting, absorption, or transport (e.g., at least 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 75%, 80%, 90% improved or more) as compared to an active agent alone. In some embodiments, a pharmaceutical composition has reduced undesirable effects, such as reduced diffusion to a nontarget location, off-target activity, or toxic metabolism, as compared to a therapeutic alone (e.g., at least 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 75%, 80%, 90% or more reduced, as compared to an active agent alone). In some embodiments, a composition increases efficacy and/or decreases toxicity of a therapeutic (e.g., at least 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 75%, 80%, 90% or more) as compared to an active agent alone.
Pharmaceutical compositions described herein may be formulated for example including a carrier, such as a pharmaceutical carrier and/or a polymeric carrier, e.g., a liposome or vesicle, and delivered by known methods to a subject in need thereof (e.g., a human or non-human agricultural or domestic animal, e.g., cattle, dog, cat, horse, poultry). Such methods include transfection (e.g., lipid-mediated, cationic polymers, calcium phosphate); electroporation or other methods of membrane disruption (e.g., nucleofection) and viral delivery (e.g., lentivirus, retrovirus, adenovirus, AAV). Methods of delivery are also described, e.g., in Gori et al., Delivery and Specificity of CRISPR/Cas9 Genome Editing Technologies for Human Gene Therapy. Human Gene Therapy. July 2015, 26(7): 443-451.
doi:10.1089/hum.2015.074; and Zuris et al. Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol. 2014 Oct 30;33(1):73-80.
Liposomes are spherical vesicle structures composed of a uni- or multilamellar lipid bilayer surrounding internal aqueous compartments and a relatively impermeable outer lipophilic phospholipid bilayer.
Liposomes may be anionic, neutral, or cationic. Liposomes are biocompatible, nontoxic, can deliver both hydrophilic and lipophilic drug molecules, protect their cargo from degradation by plasma enzymes, and transport their load across biological membranes and the blood brain barrier (BBB) (see, e.g., Spuch and Navarro, Journal of Drug Delivery, vol. 2011, Article ID 469679, 12 pages, 2011.
doi:10.1155/2011/469679 for review).
Vesicles can be made from several different types of lipids; however, phospholipids are most commonly used to generate liposomes as drug carriers. Vesicles may comprise without limitation DOTMA, DOTAP, DOTIM, DDAB, alone or together with cholesterol to yield DOTMA
and cholesterol, DOTAP and cholesterol, DOTIM and cholesterol, and DDAB and cholesterol.
Methods for preparation of multilamellar vesicle lipids are known in the art (see for example U.S.
Pat. No. 6,693,086, the teachings of which relating to multilamellar vesicle lipid preparation are incorporated herein by reference). Although vesicle formation can be spontaneous when a lipid film is mixed with an aqueous solution, it can also be expedited by applying force in the form of shaking by using a homogenizer, sonicator, or an extrusion apparatus (see, e.g., Spuch and Navarro, Journal of Drug Delivery, vol. 2011, Article ID 469679, 12 pages, 2011. doi:10.1155/2011/469679 for review).
Extruded lipids can be prepared by extruding through filters of decreasing size, as described in Templeton et al., Nature Biotech, 15:647-652, 1997, the teachings of which relating to extruded lipid preparation are incorporated herein by reference.
Methods and compositions provided herein may comprise a pharmaceutical composition administered by a regimen sufficient to alleviate a symptom of a disease, disorder, and/or condition. In some aspects, the present disclosure provides methods of delivering a therapeutic by administering compositions as described herein.
Uses The present disclosure is further directed to uses of the expression repression systems disclosed herein. Among other things, in some embodiments such provided technologies may be used to achieve modulation, e.g., repression, of target gene expression and, for example, enable control of target gene activity, delivery, and penetrance, e.g., in a cell. In some embodiments, a cell is a mammalian, e.g., human, cell. In some embodiments, a cell is a somatic cell. In some embodiments, a cell is a primary cell.
For example, in some embodiments, a cell is a mammalian somatic cell. In some embodiments, a mammalian somatic cell is a primary cell. In some embodiments, a mammalian somatic cell is a non-embryonic cell.
Dosage The dosage of the administered agent or composition can vary based on, e.g., the condition being treated, the severity of the disease, the subject's individual parameters, including age, physiological condition, size and weight, duration of treatment, the type of treatment to be performed (if any), the particular route of administration and similar factors. Thus, the dose administered of the agents described herein can depend on such various parameters. The dosage of an administered composition may also vary depending upon other factors as the subject's sex, general medical condition, and severity of the disorder to be treated. It may be desirable to provide the subject with a dosage of an expression repressor or an expression repression system or combination of expression repressors disclosed herein as circumstances dictate. In some embodiments, the subject is provided with a dosage of an expression repressor, or an expression repression system or combination of expression repressors disclosed herein as circumstances dictate.
Pharmaceutical compositions according to the present disclosure may be delivered in a therapeutically effective amount. A precise therapeutically effective amount is an amount of a composition that will yield the most effective results in terms of efficacy of treatment in a given subject. This amount will vary depending upon a variety of factors, including but not limited to characteristics of a therapeutic compound (including activity, pharmacolcinetics, pharmacodynamics, and bioavailability), physiological condition of a subject (including age, sex, disease type and stage, general physical condition, responsiveness to a given dosage, and type of medication), nature of a pharmaceutically acceptable carrier or carriers in a formulation, and/or route of administration.
Lipid Nanoparticles Expression repressors or expression repression systems as described herein can be delivered using any biological delivery system/formulation including a particle, for example, a nanoparticle delivery system.
Nanoparticles include particles with a dimension (e.g. diameter) between about 1 and about 1000 nanometers, between about 1 and about 500 nanometers in size, between about 1 and about 100 nm, between about 30 nm and about 200 nm, between about 50 nm and about 300 nm, between about 75 nm and about 200 nm, between about 100 nm and about 200 nm, and any range therebetween. A nanoparticle has a composite structure of nanoscale dimensions. In some embodiments, nanoparticles are typically spherical although different morphologies are possible depending on the nanoparticle composition. The portion of the nanoparticle contacting an environment external to the nanoparticle is generally identified as the surface of the nanoparticle. In some embodiments, nanoparticles have a greatest dimension ranging between 25 nm and 200 nm. Nanoparticles as described herein comprise delivery systems that may be provided in any form, including but not limited to solid, semi-solid, emulsion, or colloidal nanoparticles.
A nanoparticle delivery system may include but not limited to lipid-based systems, liposomes, micelles, micro-vesicles, exosomes, or gene gun. In one embodiment, the nanoparticle is a lipid nanoparticle (LNP). In some embodiments, the LNP is a particle that comprises a plurality of lipid molecules physically associated with each other by intermolecular forces.
In some embodiments, an LNP may comprise multiple components, e.g., 3-4 components. In one embodiment, the expression repressor or a pharmaceutical composition comprising said expression repressor (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repressor nucleic acid) is encapsulated in an LNP. In one embodiment, the expression repression system or a pharmaceutical composition comprising said expression repression system (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repression system nucleic acid) is encapsulated in an LNP. In some embodiments, the nucleic acid encoding the first expression repressor and the nucleic acid encoding the second expression repressor are present in same LNP. In some embodiments, the nucleic acid encoding the first expression repressor and the nucleic acid encoding the second expression repressor are present in different LNPs. Preparation of LNPs and the modulating agent encapsulation may be used/and or adapted from Rosin et al, Molecular Therapy, vol. 19, no. 12, pages 1286-2200, December 2011). In some embodiments, lipid nanoparticle compositions disclosed herein are useful for expression of protein encoded by mRNA. In some embodiments, nucleic acids, when present in the lipid nanoparticles, are resistant in aqueous solution to degradation with a nuclease.
In some embodiments, the LNP formulations may include a CCD lipid, a neutral lipid, and/or a helper lipid. In some embodiments, the LNP formulation comprises an ionizable lipid. In some embodiments, an ionizable lipid may be a cationic lipid, an ionizable cationic lipid, or an amine-containing lipid that can be readily protonated. In some embodiments, the lipid is a cationic lipid that can exist in a positively charged or neutral form depending on pH. In some embodiments, the cationic lipid is a lipid capable of being positively charged, e.g., under physiological conditions. In some embodiments, the lipid particle comprises a cationic lipid in formulation with one or more of neutral lipids, ionizable amine-containing lipids, biodegradable alkyn lipids, steroids, phospholipids including polyunsaturated lipids, structural lipids (e.g., sterols), PEG, cholesterol, and polymer conjugated lipids.
In some embodiments, LNP formulation (e.g., MC3 and/or SSOP) includes cholesterol, PEG, and/or a helper lipid. The LNPs may be, e.g., microspheres (including unilamellar and multilamellar vesicles, lamellar phase lipid bilayers that, in some embodiments, are substantially spherical.
In some embodiments, the LNP can comprise an aqueous core, e.g., comprising a nucleic acid encoding an expression repressor or a system as disclosed herein. In some embodiments of the present disclosure, the cargo for the LNP formulation includes at least one guide RNA. In some embodiments, the cargo, e.g., a nucleic acid encoding an expression repressor, or a system as disclosed herein, may be adsorbed to the surface of an LNP, e.g., an LNP comprising a cationic lipid. In some embodiments, the cargo, e.g., a nucleic acid encoding an expression repressor, or a system as disclosed herein may be associated with the LNP. In some embodiments, the cargo, e.g., a nucleic acid encoding an expression repressor, or a system as disclosed herein, may be encapsulated, e.g., fully encapsulated and/or partially encapsulated in an LNP.
In some embodiments, an LNP comprising a cargo may be administered for systemic delivery, e.g., delivery of a therapeutically effective dose of cargo that can result in a broad exposure of an active agent within an organism. Systemic delivery of lipid nanoparticles can be by any means known in the art including, for example, intravenous, intraarterial, subcutaneous, and intraperitoneal delivery. In some embodiments, systemic delivery of lipid nanoparticles is by intravenous delivery. In some embodiments, an LNP comprising a cargo may be administered for local delivery, e.g., delivery of an active agent directly to a target site within an organism.
The LNPs may be formulated as a dispersed phase in an emulsion, micelles, or an internal phase in a suspension. In some embodiments, the LNPs are biodegradable. In some embodiments, the LNPs do not accumulate to cytotoxic levels or cause toxicity in vivo at a therapeutically effective dose. In some embodiments, the LNPs do not accumulate to cytotoxic levels or cause toxicity in vivo after repeat administrations at a therapeutically effective dose. In some embodiments, the LNPs do not cause an innate immune response that leads to a substantially adverse effect at a therapeutically effective dose.
In some embodiments, the LNP used, comprises the formula (6Z,9Z,28Z,31Z)-heptatriacont-6,9,28,31-tetraene-19-y1 4-(dimethylamino) butanoate or ssPalm0-phenyl-P4C2 (ssPalmO-Phe, SS-OP). In some embodiments, the LNP formulation comprises the formula, (6Z,9Z,28Z,31Z)-heptatriacont-6,9,28,31-tetraene-19-y1 4-(dimethylamino)butanoate (MC3), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), Cholesterol, 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (PEG2k-DMG), e.g., MC3 LNP or ssPalm0-phenyl-P4C2 (ssPalmO-Phe, SS-OP), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), Cholesterol, 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (PEG2k-DMG), e.g., SSOP-LNP.
Liposomes are spherical vesicle structures composed of a uni- or multilamellar lipid bilayer surrounding internal aqueous compartments and a relatively impermeable outer lipophilic phospholipid bilayer.
Liposomes may be anionic, neutral, or cationic. Liposomes are biocompatible, nontoxic, can deliver both hydrophilic and lipophilic drug molecules, protect their cargo from degradation by plasma enzymes, and transport their load across biological membranes and the blood brain barrier (BBB) (see, e.g., Spuch and Navarro, Journal of Drug Delivery, vol. 2011, Article ID 469679, 12 pages, 2011.
doi:10.1155/2011/469679 for review).
Vesicles can be made from several different types of lipids; however, phospholipids are most commonly used to generate liposomes as drug carriers. Vesicles may comprise without limitation DOTMA, DOTAP, DOTIM, DDAB, alone or together with cholesterol to yield DOTMA
and cholesterol, DOTAP and cholesterol, DOTIM and cholesterol, and DDAB and cholesterol.
Methods for preparation of multilamellar vesicle lipids are known in the art (see for example U.S.
Pat. No. 6,693,086, the teachings of which relating to multilamellar vesicle lipid preparation are incorporated herein by reference). Although vesicle formation can be spontaneous when a lipid film is mixed with an aqueous solution, it can also be expedited by applying force in the form of shaking by using a homogenizer, sonicator, or an extrusion apparatus (see, e.g., Spuch and Navarro, Journal of Drug Delivery, vol. 2011, Article ID 469679, 12 pages, 2011. doi:10.1155/2011/469679 for review).
Extruded lipids can be prepared by extruding through filters of decreasing size, as described in Templeton et al., Nature Biotech, 15:647-652, 1997, the teachings of which relating to extruded lipid preparation are incorporated herein by reference.
Methods and compositions provided herein may comprise a pharmaceutical composition administered by a regimen sufficient to alleviate a symptom of a disease, disorder, and/or condition. In some aspects, the present disclosure provides methods of delivering a therapeutic by administering compositions as described herein.
Modulating Gene Expression The present disclosure is further directed, in part, to a method of modulating, e.g., decreasing, expression of a target gene, comprising providing an expression repressor (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repressor nucleic acid) or an expression repression system described herein (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repression system or nucleic acid), and contacting the target gene, and/or operably linked transcription control element(s) with the expression repressor or the expression repression system. In some embodiments, modulating, e.g., decreasing expression of a target gene, comprises modulation of transcription of a target gene, as compared with a reference value, e.g., transcription of a target gene, in absence of the expression repressor or the expression repression system. In some embodiments, the method of modulating, e.g., decreasing, expression of a target gene, are used ex vivo, e.g., on a cell from a subject, e.g., a mammalian subject, e.g., a human subject. In some embodiments, the method of modulating, e.g., decreasing, expression of a target gene, are used in vivo, e.g., on a mammalian subject, e.g., a human subject. In some embodiments, the method of modulating, e.g., decreasing, expression of a target gene, are used in vitro, e.g., on a cell or cell line described herein.
The present disclosure is further directed, in part to a method of treating a condition associated with over-expression of a target gene in a subject, comprising administering to the subject an expression repression system described herein (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repression system or nucleic acid). Conditions associated with over-expression of particular genes are known to those of skill in the art. Such conditions include, but are not limited to, metabolic disorders, neuromuscular disorders, cancer (e.g., solid tumors), fibrosis, diabetes, urea disorders, immune disorders, inflammation, and arthritis.
The present disclosure is further directed, in part to a method of treating a condition associated with mis-regulation of the expression of a target gene in a subject, comprising administering to the subject an expression repression system described herein (or a nucleic acid encoding the same, or pharmaceutical composition comprising said expression repression system or nucleic acid).
Without wishing to be bound by theory, it is thought that an expression repression system may be used to target (e.g., decrease expression of) a gene which modulates the expression of a target gene, thus altering expression of the target gene by altering expression of the modulating gene. Conditions associated with mis-regulation of the expression of particular genes are known to those of skill in the art.
Such conditions include, but are not limited to metabolic disorders, neuromuscular disorders, cancer (e.g., solid tumors), fibrosis, diabetes, urea disorders, immune disorders, inflammation, and arthritis.
Methods and compositions as provided herein may treat a condition associated with over-expression or mis-regulation of a target gene by stably or transiently altering (e.g., decreasing) transcription of a target gene. In some embodiments, such a modulation persists for at least about 1 hr to about 30 days, or at least about 2 hrs, 6 hrs, 12 hrs, 18 hrs, 24 hrs, 2 days, 3, days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 21 days, 22 days, 23 days, 24 days, 25 days, 26 days, 27 days, 28 days, 29 days, 30 days, or longer or any time therebetween. In some embodiments, such a modulation persists for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6, or 7 days, or at least 1, 2, 3, 4, or 5 weeks, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely). Optionally, such a modulation persists for no more than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 years.
In some embodiments, a method or composition provided herein may decrease expression of a target gene in a cell by at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100%
(and optionally up to 100%) relative to expression of the target gene in a cell not contacted by the composition or treated with the method.
In some embodiments, a method provided herein may modulate, e.g., decrease, expression of a target gene by disrupting a genomic complex, e.g., an anchor sequence-mediated conjunction, associated with said target gene. A gene that is associated with an anchor sequence-mediated conjunction may be at least partially within a conjunction (that is, situated sequence-wise between a first and second anchor sequences), or it may be external to a conjunction in that it is not situated sequence-wise between a first and second anchor sequences, but is located on the same chromosome and in sufficient proximity to at least a first or a second anchor sequence such that its expression can be modulated by controlling the topology of the anchor sequence-mediated conjunction. Those of ordinary skill in the art will understand that distance in three-dimensional space between two elements (e.g., between the gene and the anchor sequence-mediated conjunction) may, in some embodiments, be more relevant than distance in terms of base pairs. In some embodiments, an external but associated gene is located within 2 Mb, within 1.9 Mb, within 1.8 Mb, within 1.7 Mb, within 1.6 Mb, within 1.5 Mb, within 1.4 Mb, with 1.3 Mb, within 1.3 Mb, within 1.2 Mb, within 1.1 Mb, within 1 Mb, within 900 kb, within 800 kb, within 700 kb, within 500 kb, within 400 kb, within 300 kb, within 200 kb, within 100 kb, within 50 kb, within 20 kb, within 10 kb, or within 5 kb of the first or second anchor sequence.
In some embodiments, modulating expression of a gene comprises altering accessibility of a transcriptional control sequence to a gene. A transcriptional control sequence, whether internal or external to an anchor sequence-mediated conjunction, can be an enhancing sequence or a silencing (or repressive) sequence.
Epigenetic Modification The present disclosure is further directed, in part, to a method of epigenetically modifying a target gene, a transcription control element operably linked to a target gene, or an anchor sequence (e.g., an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to a target gene), the method comprising providing an expression repression system (e.g., expression repressor(s)), or nucleic acid encoding the same or pharmaceutical composition comprising said expression repression system or nucleic acid; and contacting the target gene or a transcription control element operably linked to the target gene with the expression repression system, thereby epigenetically modifying the target gene or a transcription control element operably linked to the target gene.
In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises increasing or decreasing DNA methylation of the target gene or a transcription control element operably linked to a target gene. In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises increasing or decreasing histone methylation of a histone associated with the target gene or a transcription control element operably linked to a target gene. In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises decreasing histone acetylation of a histone associated with the target gene, or a transcription control element operably linked to a target gene. In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene .. comprises increasing or decreasing histone sumoylation of a histone associated with the target gene or a transcription control element operably linked to a target gene. In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises increasing or decreasing histone phosphorylation of a histone associated with the target gene or a transcription control element operably linked to a target gene.
In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene may decrease the level of the epigenetic modification by at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% (and optionally up to 100%) relative to the level of the epigenetic modification at that site in a cell not contacted by the composition or treated with the method.
In some embodiments, a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene may increase the level of the epigenetic modification by at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 120, 140, 160, 180, 200, 300, 400, 500, 600, 700, 800, 900, or 1000% (and optionally up to 200, 300, 400, 500, 600, 700, 800, 900, 1000, or 2000%) relative to the level of the epigenetic modification at that site in a cell not contacted by the composition or treated with the method. In some embodiments epigenetic modification of a target gene or a transcription control element operably linked to a target gene may modify the level of expression of the target gene, e.g., as described herein.
In some embodiments, an epigenetic modification produced by a method described herein persists for at least about 1 hr to about 30 days, or at least about 2 hrs, 6 hrs, 12 hrs, 18 hrs, 24 hrs, 2 days, 3, days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 21 days, 22 days, 23 days, 24 days, 25 days, 26 days, 27 days, 28 days, 29 days, 30 days, or longer or any time therebetween. In some embodiments, such a modulation persists for at least 1, 2, 3,4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or at least 1, 2, 3, 4, 5, 6, or 7 days, or at least 1,2, 3, 4, or 5 weeks, or at least 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, or 12 months, or at least 1, 2, 3, 4, or 5 years (e.g., indefinitely).
Optionally, such a modulation persists for no more than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 years.
In some embodiments, an expression repression system for use in a method of epigenetically modifying a target gene or a transcription control element operably linked to a target gene comprises an expression repressor comprising a repressor domain that is or comprises an epigenetic modifying moiety.
For example, a repressor domain may be or comprise an epigenetic modifying moiety with DNA
methyltransferase activity, and an endogenous or naturally occurring target sequence (e.g. a target gene or transcription control element) may be altered to increase its methylation (e.g., decreasing interaction of a transcription factor with a portion of target gene or transcription control element, decreasing binding of a nucleating protein to an anchor sequence, and/or disrupting or preventing an anchor sequence-mediated conjunction), or may be altered to decrease its methylation (e.g., increasing interaction of a transcription factor with a portion of a target gene or transcription control element, increasing binding of a nucleating protein to an anchor sequence, and/or promoting or increasing strength of an anchor sequence-mediated conjunction).
Kits The present disclosure further directed, in part, to a kit comprising an expression repressor or an expression repression system, e.g., expression repressor(s), described herein.
In some embodiments, a kit comprises an expression repressor or an expression repression system (e.g., the expression repressor(s) of the expression repression system) and instructions for the use of said expression repressor or expression repression system. In some embodiments, a kit comprises a nucleic acid encoding the expression repressor or the expression repression system or a component thereof (e.g., the expression repressor(s) of the expression repression system) and instructions for the use of said nucleic acid and/or said expression repressor or the expression repression system. In some embodiments, a kit comprises a cell comprising a nucleic acid encoding the expression repressor or the expression repression system or a component thereof (e.g., the expression repressor(s) of the expression repression system) and instructions for the use of said cell, nucleic acid, and/or said expression repression system.
In some embodiments, a kit comprises a unit dosage of an expression repressor or an expression repression system, e.g., expression repressor(s), described herein, or a unit dosage of a nucleic acid, e.g., a vector, encoding an expression repressor or an expression repression system, e.g., expression repressor(s), described herein.
In some embodiments the kit further comprises b) a set of instructions comprising at least one method for treating a disease or modulating, e.g., decreasing the expression of target gene within a cell with said composition. In some embodiments, the kits can optionally include a delivery vehicle for said composition (e.g., a lipid nanoparticle). The reagents may be provided suspended in the excipient and/or delivery vehicle or may be provided as a separate component which can be later combined with the excipient and/or delivery vehicle. In some embodiments, the kits may optionally contain additional therapeutics to be co-administered with the compositions to affect the desired target gene expression.
While the instructional materials typically comprise written or printed materials, they are not limited to such. Any medium capable of storing such instructions and communicating them to an end user is contemplated. Such media include but are not limited to electronic storage media (e.g., magnetic discs, tapes, cartridges, chips), optical media (e.g., CD ROM), and the like. Such media may include addresses to internet sites that provide such instructional materials.
In some embodiments, a kit comprises a unit dosage of an expression repressor an expression repression system, e.g., expression repressor(s), described herein, or a unit dosage of a nucleic acid, e.g., a vector, encoding an expression repression system, e.g., expression repressor(s), described herein.
The following examples are provided to further illustrate some embodiments of the present disclosure but are not intended to limit the scope of the disclosure; it will be understood by their exemplary nature that other procedures, methodologies, or techniques known to those skilled in the art may alternatively be used.
EXAMPLES
Example 1: Target Selection and Guide Design The I3-2-microglobulin (I32M) gene was targeted for down-regulation using an expression repression system of the disclosure. A first expression repressor comprising an S. pyo genes dCas9 and a KRAB domain (Sp-dCas9-KRAB, corresponding to SEQ ID NOs: 91-93) and a second expression repressor comprising S. aureus dCas9 and MQ1 (Sa-dCas9-MQ1, corresponding to SEQ ID Nos: 94-96) were used. Guide RNAs (comprising target-binding sequences corresponding to SEQ ID NOs: 1-5) targeted each expression repressor to an area of CpG hypomethylation in the promoter region of the I32M
gene (Figure 1), where methylation runs from 0 to 1 (0 to 100%) and where higher methylation is depicted by red bars. Each bar represents a CpG site. Blue guides (GD-28228 and GD-28229) were used to target Sp-dCas9-KRAB and orange guides (GD-28171, GD-28172 and GD-28173) were used to target Sa-dCas9-MQ1 to the CpG Island in the I32M promoter region.
Example 2: I32M Expression Repression Using One or Two Expression Repressors The guide RNAs of Example 1 were used to target the Sp-dCas9-KRAB expression repressor, the Sa-dCas9-MQ1 expression repressor, or both to the promoter region of I32M and the effects on I32M
expression monitored by qPCR. mRNA encoding the expression repressor(s) and guide RNA(s) were delivered to cells in lipid nanoparticles (LNPs).
HepG2 cells were seeded in a 96 well plate at a density of 30,000 cells/well in 100 tit RPMI (+10% FBS, +Penn/Strep). Cells were allowed to attach for 24 hours prior to treatment with 0.125 ug RNA (1:1 mRNA: sgRNA by weight) per guide. RNA was delivered by LNP formulated with COATSOME SS-OP
ionizable lipid (NOF America). LNPs were formulated with one mRNA and one guide, with the exception of the samples noted as "co-formulated" which were prepared with two guides, maintaining the same mRNA: guide weight ratio. Cells were incubated with the LNPs for 72 hours, following which the cells were harvested for I32M mRNA analysis by qPCR (Figure 2), with I32M mRNA
levels given relative to untreated control cells.
dCas9 alone targeted to the I32M promoter did not decrease I32M mRNA levels.
The data shows that Sp-dCas9-KRAB or Sa-dCas9-MQ1 alone decreased I32M mRNA levels, with some variation in the decrease depending on the guide RNA(s) used. Sp-dCas9-KRAB and Sa-dCas9-MQ1 together decreased I32M
mRNA levels on average more than either expression repressor alone, again with some variation in the decrease depending on the guide RNA(s) used. Use of an expression repression system comprising one or two expression repressors decreased expression of a target gene The expression repression system comprising two expression repressors appeared to decrease expression of the target gene more than a single expression repressor.
Example 3: I32M Expression Repression Across Doses and Using Flow Cytometry Guide RNA of Example 1 (GD-28228 for Sp-dCas9 and Sp-dCas9-KRAB; GD-28172 for Sa-dCas9-MQ1; or GD-28228 and GD-28172 for Sp-dCas9-KRAB and Sa-dCas9-MQ1) was used to target the Sp-dCas9-KRAB expression repressor, the Sa-dCas9-MQ1 expression repressor, or both to the promoter region of I32M and the effects on I32M expression monitored by qPCR and also by flow cytometry (using a fluorescent I32M to monitor I32M protein levels). mRNA encoding the expression repressor(s) and guide RNA(s) were delivered to cells in LNPs at different dosages and formulations.
HepG2 cells were seeded in a 12-well plate at a density of 330,000 cells/well 1.5 mL RPMI (+10% FBS, +Penn/Strep). Cells were allowed to attach for 24 hours prior to treatment with 1.25 ug or 2.5 ug RNA
(1:1 mRNA: sgRNA by weight) per guide in 1 mL media. RNA was delivered by LNP
formulated with COATSOME SS-OP ionizable lipid (NOF America). LNPs were formulated with one mRNA and one guide, with the exception of the samples noted as "co-formulated" which were prepared with two guides, maintaining the same mRNA: guide weight ratio. Cells were incubated with the LNPs for 72 hours, following which the cells were harvested for FACS and mRNA analysis by qPCR.
As seen in Example 2, dCas9 alone targeted to the I32M promoter did not decrease I32M mRNA levels.
Sp-dCas9-KRAB or Sa-dCas9-MQ1 alone, and Sp-dCas9-KRAB and Sa-dCas9-MQ1 together decreased I32M mRNA levels (Figure 3). Both 1.25 ug/ml and 2.5 ug/ml doses of the expression repressors (alone or together) decreased I32M mRNA levels, with the higher dose of Sp-dCas9-KRAB
appearing to give a greater decrease than the lower dose. FACS monitoring of I32M protein levels showed that Sp-dCas9-KRAB or Sa-dCas9-MQ1 treated cells showed a decrease in the population of cells having the untreated level of I32M protein and the appearance of a new population of cells with a decreased level of I32M
protein. These data are consistent with those of Example 2, showing that use of an expression repression system comprising one or two expression repressors decreases expression of a target gene, and that individual expression repressors decrease the levels of a protein encoded by the target gene too.
Example 4: I32M Expression Repression Over Time Guide RNA (GD-28228 for Sp-dCas9 and Sp-dCas9-KRAB; GD-28172 for Sa-dCas9-MQ1;
or GD-28228 and GD-28172 for Sp-dCas9-KRAB and Sa-dCas9-MQ1) of Example 1 was used to target the Sp-dCas9-KRAB expression repressor, the Sa-dCas9-MQ1 expression repressor, or both to the promoter region of I32M and the effects on I32M expression monitored by qPCR. Cells were collected at different days after transfection to extract total RNA and probe for expression changes.
K562 cells were plated in a 24-well plate and transfected with MC3-based LNP
formulation enclosing:
dCas9 mRNA with I32M targeting guide RNA, Sp-dCas9-KRAB mRNA with I32M
targeting guide RNA, Sa-dCas9-MQ1 mRNA with I32M targeting guide RNA, or a mixture of Sp-dCas9-KRAB
and Sa-dCas9-MQ1 mRNAs with I32M targeting guide RNAs. Cells were collected at different days after transfection to extract total RNA and probe for expression changes in I32M mRNA (Figure 4).
Dotted lines indicate average untreated I32M mRNA levels over time (top) and average knockdown levels of I32M mRNA 24 hours post transfection (bottom).
Over a 22-day period, non-transfected (blue line) and dCas9 transfected (red line) cells don't change I32M
expression, whereas Sp-dCas9-KRAB (green line) transfected cells have lower I32M mRNA levels in the initial days post-transfection, with maximum effect on day 2, but the repression is lost after day 5. Sa-dCas9-MQ1 (purple line) transfected cells show significant repression (up to 85% by day 4) of I32M
mRNA levels and the repression is retained and then slowly the repression decreases somewhat as seen by the line remaining just under the dotted line in the figure at the end of the test period. Sp-dCas9-KRAB
and Sa-dCas9-MQ1 combined transfection (orange line) did not give any added repression than what is already observed for Sa-dCas9-MQ1 alone (purple line).
These data show that use of an expression repression system comprising one or two expression repressors decreases expression of a target gene. Individual expression repressors such as those comprising MQ1 can repress expression of a target gene for an extended duration, and expression repression systems comprising more than one expression repressor, e.g., one of which is an expression repressor comprising MQ1, can repress expression of a target gene for an extended duration as well.
Example 5: Durable repression of B2M expression after promoter methylation by DNA
methyltransferases This example demonstrates methylation of B2M promoter region by treatment with LNPs containing various DNMTs and LNPs containing a guide targeting the dCas9 fusions to the B2M
promoter region cause durable repression of B2M expression.
K-562 cells were seeded in 24 well plates at a density of 200,000 cells per well in 750 tit of growth medium (RPMI + 10% FBS + pen/strep). mRNAs encoding effectors, dCas9-MQ1(SEQ ID NO:
33), dCas9-DNMT3a/3L (m) ("m" denoting that the 3a region has the human sequence but the 3L region has the mouse sequence) (SEQ ID NO:36), dCas9-DNMT3a/3L (h ("h" denoting that both the 3a region and the 3L region have the human sequences) (SEQ ID NO:35), dCas9-DNMT1(SEQ ID
NO: 35), and dCas9-DNMT3B were formulated in MC3 lipids using a Precision NanoSystems NanoAssemblr Spark instrument. Single guide RNAs (sgRNAs) of SEQ ID NO: 21 were formulated in the same lipids in the same instrument in separate formulations. Stock LNP concentrations were 100 tig/mL. Cells were treated with LNPs encapsulating mRNAs and LNPs encapsulating sgRNAs in a 1:1 ratio at a final lipid concentration of 1.25 tig/mL and incubated thereafter. Each effector and guide combination were transfected in quadruplicate. At different timepoints after transfection, 200 tiL of cells were removed and processed to extract total RNA and probe for expression changes and 200 tit of cells were moved into 550 tit of fresh media. This procedure continued until 18 days after the initial transfection. RNA was extracted using the RNeasy 96 Plus kits (Qiagen), converted to cDNA using LunaScript RT SuperMix (New England Biolabs) and used for RT-qPCR using a B2M specific TaqMan primer/probe set Assay with and the TaqMan' Fast Advanced Master Mix (Thermo Fisher Scientific). Data is presented as fold change compared to un-transfected control using the ddCT method.
Results show, all the constructs described in this Example were able to repress B2M expression ranging from about 35% to 60%, 4 days after treatment relative to an untreated sample (Figure 5). All the constructs were able to repress B2M expression ranging from about 15% to 30%
at the end of 8 days relative to an untreated sample. At the end of 13 days, all effectors were able to repress B2M expression by about 45-55%, and at the end of 18 days, all effectors were able to repress B2M expression by about 30-40% relative to an untreated sample (Figure 5).
Example 6: Identification of durable repressors or repressor systems that downregulate MYC
expression by targeting a CTCF site located upstream of MYC gene in K-562 cells This example demonstrates identification of individual expression repressors or expression repression systems that downregulate MYC expression by targeting the CTCF site located upstream of MYC gene.
K-562 cells were grown in 96 well plates at a density of 1 x106 cell/mL in 100 tiL of growth medium (RPMI + 10% FBS + pen/strep). A sgRNA pool was prepared by mixing GD-29639 (SEQ ID
NO: 11) and GD-29640 (SEQ ID NO: 12) in 1:1 ratio. A repression master plate was prepared by adding 5 iu.1 of mRNAs encoding effectors or effector combinations from Table 4 in each well. After addition, 17.5 ng of each mRNA or combination of mRNAs were present in each well. After plating the master plates were stored frozen until the time of transfection.
Table 4: List of Expression repressors or expression repression systems tested Expression repressors or expression repression systems 1 No effector (Untreated) 2 dCas9 3 dCas9-DNMT1 4 dCas9-DNMT3B
5 G9A-dCas9 6 dCas9-MQ1 7 dCas9-HDAC8 8 dCas9-DNMT3a/3L (h) 9 dCas9-DNMT3a/3L (m) EZH2-dCas9 11 dCas9-LSD1 12 dCas9-KRAB
13 EZH2-dCas9-KRAB
14 G9A-dCas9-KRAB
FOG1-dCas9- FOG1 16 dCas9-KRAB + FOG1-dCas9- FOG1 17 EZH2-dCas9-KRAB + dCas9-HDAC8 18 dCas9-LSD1 + dCas9-HDAC8 19 G9A-dCas9-KRAB + EZH2-dCas9-KRAB
G9A-dCas9 + dCas9-HDAC8 21 EZH2-dCas9 + dCas9-HDAC8 22 dCas9-LSD1 + G9A-dCas9-KRAB
23 dCas9-LSD1 + EZH2-dCas9-KRAB
24 dCas9-LSD1 + EZH2-dCas9 G9A-dCas9-KRAB + dCas9-HDAC8
26 G9A-dCas9 + EZH2-dCas9
27 dCas9-MQ1 + EZH2-dCas9-KRAB
28 dCas9-LSD1 + G9A-dCas9
29 dCas9-MQ1 + dCas9-HDAC8 dCas9-MQ1 + G9A-dCas9-KRAB
Prior to transfection, 5 .1 of sgRNA pool were added in each well so that 8.75 ng of each sgRNA were present in each well. The effector mRNA-sgRNA mix were diluted with Opti-MEM
media (Thermo 5 Fisher) to a total volume of 40 .1 in each well. A transfection master mix of Lipofectamine' MessengarMAX' (Thermo Fisher) and Opti-MEM were prepared using a ratio of 0.3 tiL of Lipofectamine MessengarMAX' per 100 ng of total RNA as described in Table 5.
Immediately before transfection, 35 tiL of transfection master mix were added to each well containing RNA to bring the final volume to 75 il and were incubated for 5 minutes at room temperature and was used to transfect K-562 cells. After transfection the cells were incubated at 37 C with 5% CO2 in standard incubator.
Table 5 Reagent Per well Per 96-well plate Per 2, 96-well Per 4, 96-well plates plates MessengerMax 4.2 189 378 756 ( L) Optimem ( L) 30.8 1386 2772 5544 24 hours after transfection, 100 tiL of fresh media was added to each well.
Sample collection started 48 hours post transfection and continued until 6 days. Cell suspension samples were collected at 48 hours, 72 hours, and 144-hour time point. Each time samples were removed for analysis equivalent volumes of fresh media was added and the incubations were continued. RNA was extracted using the Rneasy Plus 96 kits (Qiagen), converted to cDNA using LunaScript RT SuperMix (New England Biolabs) and used for RT-qPCR using a MYC specific Taqman primer/probe set assay with the Taqman' Fast Advanced Master Mix (Thermo Scientific). Data was presented as fold change compared to dCas9 treated control using the ddCT method. The untreated and dCas9 samples were used as calibrators.
The experiment showed that, at least, cells treated with dCas9-DNMT3a/3L (h), dCas9-KRAB, FOG1-dCas9-FOG1, G9A-dCas9 + EZH2-dCas9, dCas9-MQ1 + EZH2-dCas9-KRAB, dCas9-LSD1+G9A-dCas9, dCas9-MQ1+dCas9-HDAC8, and dCas9-MQ1+G9A-dCas9-KRAB showed repression of MYC expression at 48 hour and/or 72-hour time point (Figure 6).
Example 7: Identification of durable repressors or repression systems that downregulate I32M
expression by targeting a region upstream of the I32M transcription start site in K-562 cells This example demonstrates identification of individual expression repressors or expression repression systems that downregulate B2M expression by targeting the upstream region of I32M promoter.
K-562 cells were grown in 96 well plates at a density of lx106 cell/mL in 100 tiL of growth medium (RPMI + 10% FBS + pen/strep). A sgRNA pool was prepared by mixing GD-27634 (SEQ ID
NO: 13) and GD-29500 (SEQ ID NO: 14) in 1:1 ratio. The effectors as described in Table X and sgRNA
pool mix was used to transfect the cells following the protocol described in Example 6. Cell suspension samples were collected at 48 hours, 72 hours, 144-hour, 192 hours, and 240 hour time point and were processed using the protocol described in Example 6. Data was presented as fold change compared to dCas9 treated control using the ddCT method. The untreated and dCas9 samples were used as calibrators.
The data showed that, at least, cells treated with dCas9-HDAC8, dCas9-LSD1, dCas9-MQ1, FOG1-dCas9- FOG1, G9A-dCas9-KRAB, dCas9-DNMT3a/3L (h), G9A-dCas9, EZH2-dCas9-KRAB, dCas9-KRAB, EZH2-dCas9-KRAB + dCas9-HDAC8, dCas9-LSD1 + EZH2-dCas9-KRAB, dCas9-LSD1 + dCas9-HDAC8, dCas9-KRAB + FOG1-dCas9- FOG1, dCas9-MQ1 + G9A-dCas9-KRAB, dCas9 + EZH2-dCas9, dCas9-MQ1 + EZH2-dCas9-KRAB, and dCas9-MQ1 + dCas9-HDAC8 showed repression of I32M expression at 48 hour, 72 hour and/or 144 hour time point (Figure 7).
Example 8: Identification of durable repressors or repression systems that downregulate HSPA1B
expression by targeting a region downstream of the HSPA1B transcription start site in K-562 cells This example demonstrates identification of individual expression repressors or expression repression systems that downregulate HSPA1B expression by targeting the downstream region of HSPA1B
promoter.
K-562 cells were grown in 96 well plates at a density of 1x106 cell/mL in 100 tiL of growth medium (RPMI + 10% FBS + pen/strep). A sgRNA pool was prepared by mixing GD-29542 (SEQ ID
NO: 17) and GD-29544 (SEQ ID NO: 18) in 1:1 ratio. The effectors as described in Table 4 and sgRNA
pool mix was used to transfect the cells following the protocol described in Example 6. Cell suspension samples were collected at 48 hours, 72 hours, and 144-hour time point and were processed using the protocol described in Example 6. Data was presented as fold change compared to dCas9 treated control using the ddCT method. The untreated and dCas9 samples were used as calibrators.
The data showed that, at least, cells treated with dCas9-HDAC8, EZH2-dCas9, dCas9-MQ1, dCas9-DNMT1, dCas9-DNMT3a/3L (h), dCas9-DNMT3a/3L (m), G9A-dCas9-KRAB, FOG1-dCas9-FOG1, G9A-dCas9, dCas9-KRAB, G9A-dCas9 + dCas9-HDAC8, dCas9-LSD1 + G9A-dCas9-KRAB, dCas9-LSD1+EZH2-dCas9, G9A-dCas9+EZH2-dCas9, dCas9-LSD1 + EZH2-dCas9-KRAB, dCas9 +dCas9-HDAC8, dCas9-LSD1+dCas9-HDAC8, dCas9-MQ1 + EZH2-dCas9-KRAB, dCas9-MQ1+dCas9-HDAC8, G9A-dCas9-KRAB+EZH2-dCas9-KRAB, dCas9-LSD1+G9A-dCas9, and dCas9-MQ1+G9A-dCas9-KRAB showed repression of HSPA1B expression at 48 hour, 72 hour and/or 144 hour time point (Figure 8).
Example 9: Identification of durable repressors or repression systems that downregulate GATA1 expression in K-562 cells This example demonstrates identification of individual expression repressors or expression repression systems that downregulate GATA1 expression in K-562 cells.
K-562 cells were grown in 96 well plates at a density of 1 x106 cell/mL in 100 L of growth medium (RPMI + 10% FBS + pen/strep). A sgRNA pool was prepared by mixing GD-29536 (SEQ ID
NO: 19) and GD-29693 (SEQ ID NO: 20) in 1:1 ratio. The effectors as described in Table 4 and sgRNA
pool mix was used to transfect the cells following the protocol described in Example 6. Cell suspension samples were collected at 48 hours, 72 hours, and 144-hour time point and were processed using the protocol described in Example 6. Data was presented as fold change compared to dCas9 treated control using the ddCT method. The untreated and dCas9 samples were used as calibrators.
The data showed that, at least, cells treated with dCas9-HDAC8, dCas9-LSD1+dCas9-HDAC8, dCas9-MQ1+dCas9-HDAC8, and G9A-dCas9+dCas9-HDAC8 showed repression of GATA1 expression at 48-hour, 72 hour and/or 144 hour time point (Figure 9).
EQUIVALENTS
It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Some aspects, advantages, and modifications are within the scope of the following claims.
Prior to transfection, 5 .1 of sgRNA pool were added in each well so that 8.75 ng of each sgRNA were present in each well. The effector mRNA-sgRNA mix were diluted with Opti-MEM
media (Thermo 5 Fisher) to a total volume of 40 .1 in each well. A transfection master mix of Lipofectamine' MessengarMAX' (Thermo Fisher) and Opti-MEM were prepared using a ratio of 0.3 tiL of Lipofectamine MessengarMAX' per 100 ng of total RNA as described in Table 5.
Immediately before transfection, 35 tiL of transfection master mix were added to each well containing RNA to bring the final volume to 75 il and were incubated for 5 minutes at room temperature and was used to transfect K-562 cells. After transfection the cells were incubated at 37 C with 5% CO2 in standard incubator.
Table 5 Reagent Per well Per 96-well plate Per 2, 96-well Per 4, 96-well plates plates MessengerMax 4.2 189 378 756 ( L) Optimem ( L) 30.8 1386 2772 5544 24 hours after transfection, 100 tiL of fresh media was added to each well.
Sample collection started 48 hours post transfection and continued until 6 days. Cell suspension samples were collected at 48 hours, 72 hours, and 144-hour time point. Each time samples were removed for analysis equivalent volumes of fresh media was added and the incubations were continued. RNA was extracted using the Rneasy Plus 96 kits (Qiagen), converted to cDNA using LunaScript RT SuperMix (New England Biolabs) and used for RT-qPCR using a MYC specific Taqman primer/probe set assay with the Taqman' Fast Advanced Master Mix (Thermo Scientific). Data was presented as fold change compared to dCas9 treated control using the ddCT method. The untreated and dCas9 samples were used as calibrators.
The experiment showed that, at least, cells treated with dCas9-DNMT3a/3L (h), dCas9-KRAB, FOG1-dCas9-FOG1, G9A-dCas9 + EZH2-dCas9, dCas9-MQ1 + EZH2-dCas9-KRAB, dCas9-LSD1+G9A-dCas9, dCas9-MQ1+dCas9-HDAC8, and dCas9-MQ1+G9A-dCas9-KRAB showed repression of MYC expression at 48 hour and/or 72-hour time point (Figure 6).
Example 7: Identification of durable repressors or repression systems that downregulate I32M
expression by targeting a region upstream of the I32M transcription start site in K-562 cells This example demonstrates identification of individual expression repressors or expression repression systems that downregulate B2M expression by targeting the upstream region of I32M promoter.
K-562 cells were grown in 96 well plates at a density of lx106 cell/mL in 100 tiL of growth medium (RPMI + 10% FBS + pen/strep). A sgRNA pool was prepared by mixing GD-27634 (SEQ ID
NO: 13) and GD-29500 (SEQ ID NO: 14) in 1:1 ratio. The effectors as described in Table X and sgRNA
pool mix was used to transfect the cells following the protocol described in Example 6. Cell suspension samples were collected at 48 hours, 72 hours, 144-hour, 192 hours, and 240 hour time point and were processed using the protocol described in Example 6. Data was presented as fold change compared to dCas9 treated control using the ddCT method. The untreated and dCas9 samples were used as calibrators.
The data showed that, at least, cells treated with dCas9-HDAC8, dCas9-LSD1, dCas9-MQ1, FOG1-dCas9- FOG1, G9A-dCas9-KRAB, dCas9-DNMT3a/3L (h), G9A-dCas9, EZH2-dCas9-KRAB, dCas9-KRAB, EZH2-dCas9-KRAB + dCas9-HDAC8, dCas9-LSD1 + EZH2-dCas9-KRAB, dCas9-LSD1 + dCas9-HDAC8, dCas9-KRAB + FOG1-dCas9- FOG1, dCas9-MQ1 + G9A-dCas9-KRAB, dCas9 + EZH2-dCas9, dCas9-MQ1 + EZH2-dCas9-KRAB, and dCas9-MQ1 + dCas9-HDAC8 showed repression of I32M expression at 48 hour, 72 hour and/or 144 hour time point (Figure 7).
Example 8: Identification of durable repressors or repression systems that downregulate HSPA1B
expression by targeting a region downstream of the HSPA1B transcription start site in K-562 cells This example demonstrates identification of individual expression repressors or expression repression systems that downregulate HSPA1B expression by targeting the downstream region of HSPA1B
promoter.
K-562 cells were grown in 96 well plates at a density of 1x106 cell/mL in 100 tiL of growth medium (RPMI + 10% FBS + pen/strep). A sgRNA pool was prepared by mixing GD-29542 (SEQ ID
NO: 17) and GD-29544 (SEQ ID NO: 18) in 1:1 ratio. The effectors as described in Table 4 and sgRNA
pool mix was used to transfect the cells following the protocol described in Example 6. Cell suspension samples were collected at 48 hours, 72 hours, and 144-hour time point and were processed using the protocol described in Example 6. Data was presented as fold change compared to dCas9 treated control using the ddCT method. The untreated and dCas9 samples were used as calibrators.
The data showed that, at least, cells treated with dCas9-HDAC8, EZH2-dCas9, dCas9-MQ1, dCas9-DNMT1, dCas9-DNMT3a/3L (h), dCas9-DNMT3a/3L (m), G9A-dCas9-KRAB, FOG1-dCas9-FOG1, G9A-dCas9, dCas9-KRAB, G9A-dCas9 + dCas9-HDAC8, dCas9-LSD1 + G9A-dCas9-KRAB, dCas9-LSD1+EZH2-dCas9, G9A-dCas9+EZH2-dCas9, dCas9-LSD1 + EZH2-dCas9-KRAB, dCas9 +dCas9-HDAC8, dCas9-LSD1+dCas9-HDAC8, dCas9-MQ1 + EZH2-dCas9-KRAB, dCas9-MQ1+dCas9-HDAC8, G9A-dCas9-KRAB+EZH2-dCas9-KRAB, dCas9-LSD1+G9A-dCas9, and dCas9-MQ1+G9A-dCas9-KRAB showed repression of HSPA1B expression at 48 hour, 72 hour and/or 144 hour time point (Figure 8).
Example 9: Identification of durable repressors or repression systems that downregulate GATA1 expression in K-562 cells This example demonstrates identification of individual expression repressors or expression repression systems that downregulate GATA1 expression in K-562 cells.
K-562 cells were grown in 96 well plates at a density of 1 x106 cell/mL in 100 L of growth medium (RPMI + 10% FBS + pen/strep). A sgRNA pool was prepared by mixing GD-29536 (SEQ ID
NO: 19) and GD-29693 (SEQ ID NO: 20) in 1:1 ratio. The effectors as described in Table 4 and sgRNA
pool mix was used to transfect the cells following the protocol described in Example 6. Cell suspension samples were collected at 48 hours, 72 hours, and 144-hour time point and were processed using the protocol described in Example 6. Data was presented as fold change compared to dCas9 treated control using the ddCT method. The untreated and dCas9 samples were used as calibrators.
The data showed that, at least, cells treated with dCas9-HDAC8, dCas9-LSD1+dCas9-HDAC8, dCas9-MQ1+dCas9-HDAC8, and G9A-dCas9+dCas9-HDAC8 showed repression of GATA1 expression at 48-hour, 72 hour and/or 144 hour time point (Figure 9).
EQUIVALENTS
It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Some aspects, advantages, and modifications are within the scope of the following claims.
Claims (42)
1. An expression repression system comprising:
a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain, wherein the first DNA-targeting moiety specifically binds a first DNA
sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence, and wherein the first repressor domain is different from the second repressor domain.
a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain, wherein the first DNA-targeting moiety specifically binds a first DNA
sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence, and wherein the first repressor domain is different from the second repressor domain.
2. An expression repression system comprising:
a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain, wherein the first or second repressor domain comprises an MQ1 domain or functional variant or fragment thereof, wherein the first repressor domain is different from the second repressor domain.
a first expression repressor comprising a first DNA-targeting moiety and a first repressor domain, and a second expression repressor comprising a second DNA-targeting moiety and a second repressor domain, wherein the first or second repressor domain comprises an MQ1 domain or functional variant or fragment thereof, wherein the first repressor domain is different from the second repressor domain.
3. The expression repression system of claim 2, wherein:
the first DNA-targeting moiety specifically binds a first DNA sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence; or the first DNA-targeting moiety and the second DNA-targeting moiety specifically bind the same DNA sequence.
the first DNA-targeting moiety specifically binds a first DNA sequence, and the second DNA-targeting moiety specifically binds a second DNA sequence different from the first DNA sequence; or the first DNA-targeting moiety and the second DNA-targeting moiety specifically bind the same DNA sequence.
4. The expression repression system of any of claims 1-3, wherein the first DNA-targeting moiety comprises a first CRISPR/Cas molecule comprising a first CRISPR/Cas protein and first guide RNA, and the second DNA-targeting moiety comprises a second CRISPR/Cas molecule comprising a second CRISPR/Cas protein and a second guide RNA.
5. The expression repression system of any of claims 1-4:
wherein the first CRISPR/Cas protein comprises a different amino acid sequence than the second CRISPR/Cas protein.
wherein the first CRISPR/Cas protein comprises a different amino acid sequence than the second CRISPR/Cas protein.
6. The expression repression system of any of claims 1-5, wherein the first or second DNA-targeting moiety comprises a CRISPR/Cas molecule comprising a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof.
7. The expression repression system of any of claims 1-6, wherein the first DNA-targeting moiety comprises a first CRISPR/Cas molecule comprising a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof, and the second DNA-targeting moiety comprises a second CRISPR/Cas molecule comprising a different Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof.
8. The expression repression system of any of claims 1-7, wherein the first or second repressor domain comprises a histone methyltransferase activity (e.g., a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), 5UV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, or a functional variant or fragment of any thereof, e.g., a SET
domain of any thereof), a histone demethylase activity (e.g., a protein chosen from KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, or a functional variant or fragment of any thereof), a histone deacetylase activity (e.g., a protein chosen from HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof), a DNA methyltransferase activity (e.g., a protein chosen from MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, or a functional variant or fragment of any thereof), or a transcription repressor activity (e.g., a protein chosen from KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment of any thereof).
domain of any thereof), a histone demethylase activity (e.g., a protein chosen from KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, or a functional variant or fragment of any thereof), a histone deacetylase activity (e.g., a protein chosen from HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, or a functional variant or fragment of any thereof), a DNA methyltransferase activity (e.g., a protein chosen from MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, or a functional variant or fragment of any thereof), or a transcription repressor activity (e.g., a protein chosen from KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment of any thereof).
9. The expression repression system of any of claims 1-8, wherein:
the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), 5UV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
the first repressor domain comprises a protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), SUV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof, and the second repressor domain comprises a different protein chosen from SETDB1, SETDB2, EHMT2 (i.e., G9A), EHMT1 (i.e., GLP), 5UV39H1, EZH2, EZH1, 5UV39H2, SETD8, SUV420H1, 5UV420H2, KDM1A (i.e., LSD1), KDM1B (i.e., LSD2), KDM2A, KDM2B, KDM5A, KDM5B, KDM5C, KDM5D, KDM4B, N066, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7, SIRT8, SIRT9, MQ1, DNMT1, DNMT3A1, DNMT3A2, DNMT3B1, DNMT3B2, DNMT3B3, DNMT3B4, DNMT3B5, DNMT3B6, DNMT3L, KRAB, MeCP2, HP1, RBBP4, REST, FOG1, SUZ12, or a functional variant or fragment thereof.
10. The expression repression system of any of claims 1-9, wherein the first repressor domain comprises KRAB, and the second repressor domain comprises DNMT3A (e.g., human DNMT3A).
11. The expression repression system of any of claims 1-9, wherein the first repressor domain comprises KRAB, and the second repressor domain comprises bacterial MQ1.
12. The expression repression system of any of claims 1-11, wherein the first or second DNA-targeting moiety is selected from a TAL effector molecule, a CRISPR/Cas molecule, a zinc finger domain, a tetR domain, a meganuclease, or an oligonucleotide.
13. The expression repression system of any of claims 1-12, wherein the first or second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein (e.g., that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof) and a guide RNA.
14. The expression repression system of any of claims 1-13, wherein:
the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof), and the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a different CRISPR/Cas protein that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof).
the first DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a CRISPR/Cas protein that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof), and the second DNA-targeting moiety is or comprises a CRISPR/Cas molecule comprising a different CRISPR/Cas protein that is or comprises a Cas protein or Cpfl protein chosen from Table 1 or a variant (e.g., mutant) of any thereof).
15. The expression repression system of any of claims 1-14, wherein the first or the second DNA-targeting moiety specifically binds to:
a promoter sequence or to DNA proximal to a promoter sequence, e.g., a promoter sequence operably linked to a target gene;
an enhancer sequence or to DNA proximal to an enhancer sequence, e.g., an enhancer sequence that affects expression of and/or is operably linked to a target gene;
a target gene, e.g., sequence comprised between the transcription start site and stop codon of a target gene;
the transcription start site of a target gene;
an intron, e.g., a splice site associated with an intron, of a target gene; or an exon of a target gene.
a promoter sequence or to DNA proximal to a promoter sequence, e.g., a promoter sequence operably linked to a target gene;
an enhancer sequence or to DNA proximal to an enhancer sequence, e.g., an enhancer sequence that affects expression of and/or is operably linked to a target gene;
a target gene, e.g., sequence comprised between the transcription start site and stop codon of a target gene;
the transcription start site of a target gene;
an intron, e.g., a splice site associated with an intron, of a target gene; or an exon of a target gene.
16. The expression repression system of any of claims 1-15, wherein the first DNA sequence is a first site within or proximal to a promoter operably linked to a target gene and the second DNA sequence is a second site within or proximal to the promoter operably linked to the target gene.
17. The expression repression system of any of claims 1-16, wherein the distance between the first DNA sequence and the second DNA sequence is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 base pairs or any size therebetween, e.g., 20-500 base pairs.
18. The expression repression system of any of claims 1-17, wherein the distance between the first DNA sequence and the second DNA sequence is no more than 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 base pairs or any size therebetween, e.g., 20-500 base pairs.
19. The expression repression system of any of claims 1-18, wherein the target gene is 13-2-microglobulin, MYC, HSPA1B, GATA1, APOB, FOXP3, CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL6, CXCL7, and/or CXCL8.
20. The expression repression system of any of claims 1-19, wherein the first DNA-targeting moiety does not appreciably bind to the second DNA sequence and the second DNA-targeting moiety does not appreciably bind to the first DNA sequence, e.g., wherein the first DNA-targeting moiety binds the second DNA sequence with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or .. 100,000 nM, and the second DNA-targeting moiety binds the first DNA
sequence with binds with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or 100,000 nM.
sequence with binds with a KD of at least 500, 600, 700, 800, 900, 1000, 2000, 5000, 10,000, or 100,000 nM.
21. The expression repression system of any of claims 1-20, wherein binding of the first expression repressor to the first DNA sequence and/or binding of the second expression repressor to the second DNA
sequence decreases expression of a target gene, e.g., a target gene operably linked to the first and/or second DNA sequence, in a cell, e.g., by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the first and/or second expression repressor.
sequence decreases expression of a target gene, e.g., a target gene operably linked to the first and/or second DNA sequence, in a cell, e.g., by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the first and/or second expression repressor.
22. The expression repression system of any of claims 1-21, wherein binding of the first and/or second expression repressor to the first and/or second DNA sequence appreciably decreases expression of a target gene, e.g., a target gene operably linked to the first and/or second DNA sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
23. The expression repression system of either of claims 21 or 22, wherein expression is decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in the absence of the first and second expression repressors, e.g., as measured by ELISA or as described in Examples 2-4.
24. The expression repression system of any of claims 21-23, wherein binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA sequence appreciably decreases expression of a target gene, e.g., a target gene operably linked to the first DNA
sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 hours, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
sequence, for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 hours, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
25. The expression repression system of any of claims 21-24, wherein the decrease in expression resulting from the binding of the first expression repressor to the first DNA
sequence and the second expression repressor to the second DNA sequence is greater than the decrease in expression resulting from the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually.
sequence and the second expression repressor to the second DNA sequence is greater than the decrease in expression resulting from the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually.
26. The expression repression system of claim 25, wherein the binding of the first expression .. repressor to the first DNA sequence and the second expression repressor to the second DNA sequence decreases expression 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 50x, or 100x more than either the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually, e.g., as measured by ELISA or as described in Examples 2-4.
27. The expression repression system of any of claim 21-26, wherein the decrease in expression resulting from the binding of the first expression repressor to the first DNA
sequence and the second expression repressor to the second DNA sequence persists for a longer time (e.g., more hours, days, or cell divisions) than the decrease in expression resulting from the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually.
sequence and the second expression repressor to the second DNA sequence persists for a longer time (e.g., more hours, days, or cell divisions) than the decrease in expression resulting from the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually.
28. The expression repression system of claim 27, wherein the binding of the first expression repressor to the first DNA sequence and the second expression repressor to the second DNA sequence decreases expression 1.05x (i.e., 1.05 times), 1.1x, 1.15x, 1.2x, 1.25x, 1.3x, 1.35x, 1.4x, 1.45x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 3x, 4x, 5x, 6x, 7x, 8x, 9x, 10x, 20x, 50x, or 100x longer (e.g., as measured in hours, days, or cell divisions) than either the binding of the first expression repressor to the first DNA sequence or the binding of the second expression repressor to the second DNA sequence individually, e.g., as measured by ELISA or as described in Examples 2-4.
29. The expression repression system of any of claims 1-28, wherein the first expression repressor comprises a first DNA-targeting moiety comprising a CRISPR/Cas molecule comprising an S. pyogenes CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. pyogenes dCas9, and a first repressor domain comprising KRAB or a functional variant or fragment thereof; and the second expression repressor comprises a second DNA-targeting moiety comprising a CRISPR/Cas molecule comprising a S. aureus CRISPR/Cas protein selected from Table 1 or variant (e.g., mutant) thereof, e.g., an S. aureus dCas9, and a second repressor domain comprising bacterial MQ1 or a functional variant or fragment thereof.
30. A polypeptide comprising:
a DNA-targeting moiety; and a repressor domain comprising bacterial MQ1 or a functional variant or fragment thereof.
a DNA-targeting moiety; and a repressor domain comprising bacterial MQ1 or a functional variant or fragment thereof.
31. A nucleic acid encoding:
the first expression repressor, the second expression repressor, the expression repression system (e.g., the first expression repressor and the second expression repressor), or the polypeptide of any of claims 1-30.
the first expression repressor, the second expression repressor, the expression repression system (e.g., the first expression repressor and the second expression repressor), or the polypeptide of any of claims 1-30.
32. A vector comprising the nucleic acid of claim 31.
33. A cell comprising the expression repression system, nucleic acid, vector, or polypeptide of any of claims 1-32.
34. A pharmaceutical composition comprising the expression repression system, nucleic acid, polypeptide, or vector of any of claims 1-32, and at least one pharmaceutically acceptable excipient or carrier.
35. A method of decreasing expression of a target genomic sequence in a cell, comprising:
providing the expression repression system, nucleic acid, polypeptide, pharmaceutical composition, or vector of any of claims 1-32 or 34; and contacting the cell with the expression repression system, nucleic acid, polypeptide, pharmaceutical composition, or vector, thereby decreasing expression of the target genomic sequence.
providing the expression repression system, nucleic acid, polypeptide, pharmaceutical composition, or vector of any of claims 1-32 or 34; and contacting the cell with the expression repression system, nucleic acid, polypeptide, pharmaceutical composition, or vector, thereby decreasing expression of the target genomic sequence.
36. A method of epigenetically modifying a target genomic sequence in a cell, comprising:
providing the expression repression system, nucleic acid, polypeptide, pharmaceutical composition, or vector of any of claims 1-32 or 34; and contacting the cell with the expression repression system, nucleic acid, vector, polypeptide, or pharmaceutical composition, thereby epigenetically modifying the target genomic sequence.
providing the expression repression system, nucleic acid, polypeptide, pharmaceutical composition, or vector of any of claims 1-32 or 34; and contacting the cell with the expression repression system, nucleic acid, vector, polypeptide, or pharmaceutical composition, thereby epigenetically modifying the target genomic sequence.
37. The method of either of claims 35 or 36, wherein the target genomic sequence is:
a target gene (e.g., a site within a target gene, e.g., an exon, intron, or splice site), e.g., the gene encoding I3-2-microg1obu1in (I32M), a transcription control element operably linked to a target gene, or an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to the target gene.
a target gene (e.g., a site within a target gene, e.g., an exon, intron, or splice site), e.g., the gene encoding I3-2-microg1obu1in (I32M), a transcription control element operably linked to a target gene, or an anchor sequence proximal to a target gene or associated with an anchor sequence-mediated conjunction operably linked to the target gene.
38. The method of any of claims 35-37, wherein expression is:
decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in an otherwise similar cell in the absence of the expression repression system; and/or decreased for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
decreased by 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% compared to expression in an otherwise similar cell in the absence of the expression repression system; and/or decreased for a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cell divisions, e.g., as measured by ELISA or as described in Examples 2-4.
39. The method of any of claims 35-38, wherein the decrease in expression comprises a decrease in:
the level of RNA, e.g., mRNA, encoded by the target gene; and/or the level of a protein encoded by the target gene.
the level of RNA, e.g., mRNA, encoded by the target gene; and/or the level of a protein encoded by the target gene.
40. The method of any of claims 35-39, wherein providing comprises:
contacting the cell with a nucleic acid, e.g., vector, encoding the expression repression system to the cell comprising the target gene; or contacting the cell with a lipid nanoparticle (LNP) comprising the expression repression system or a nucleic acid, e.g., vector, encoding the expression repression system.
contacting the cell with a nucleic acid, e.g., vector, encoding the expression repression system to the cell comprising the target gene; or contacting the cell with a lipid nanoparticle (LNP) comprising the expression repression system or a nucleic acid, e.g., vector, encoding the expression repression system.
41. A method of treating a condition associated with over-expression or mis-regulation of a target gene in a subject, comprising:
administering the expression repression system, nucleic acid, polypeptide, pharmaceutical composition, or vector of any of claims 1-32 or 34 to the subject, thereby treating a condition associated with over-expression of a target gene in a subject.
administering the expression repression system, nucleic acid, polypeptide, pharmaceutical composition, or vector of any of claims 1-32 or 34 to the subject, thereby treating a condition associated with over-expression of a target gene in a subject.
42. A method of making a cell comprising an expression repression system, the method comprising:
providing the expression repression system, nucleic acid, pharmaceutical composition, or vector of any of claims 1-29, 31, 32 or 34; and contacting the cell with the expression repression system, nucleic acid, vector, or pharmaceutical composition, thereby making a cell comprising an expression repression system.
providing the expression repression system, nucleic acid, pharmaceutical composition, or vector of any of claims 1-29, 31, 32 or 34; and contacting the cell with the expression repression system, nucleic acid, vector, or pharmaceutical composition, thereby making a cell comprising an expression repression system.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063082555P | 2020-09-24 | 2020-09-24 | |
| US63/082,555 | 2020-09-24 | ||
| US63/242,957 | 2021-09-10 | ||
| PCT/US2021/051945 WO2022067033A1 (en) | 2020-09-24 | 2021-09-24 | Compositions and methods for inhibiting gene expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3196827A1 true CA3196827A1 (en) | 2022-03-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3196827A Pending CA3196827A1 (en) | 2020-09-24 | 2021-09-24 | Compositions and methods for inhibiting gene expression |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA3196827A1 (en) |
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2021
- 2021-09-24 CA CA3196827A patent/CA3196827A1/en active Pending
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