CA3193089A1 - Constructs and uses thereof for efficient and specific genome editing - Google Patents
Constructs and uses thereof for efficient and specific genome editingInfo
- Publication number
- CA3193089A1 CA3193089A1 CA3193089A CA3193089A CA3193089A1 CA 3193089 A1 CA3193089 A1 CA 3193089A1 CA 3193089 A CA3193089 A CA 3193089A CA 3193089 A CA3193089 A CA 3193089A CA 3193089 A1 CA3193089 A1 CA 3193089A1
- Authority
- CA
- Canada
- Prior art keywords
- sequence
- seq
- amino acid
- nucleic acid
- nuclease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063080552P | 2020-09-18 | 2020-09-18 | |
| US63/080,552 | 2020-09-18 | ||
| US202163185315P | 2021-05-06 | 2021-05-06 | |
| US63/185,315 | 2021-05-06 | ||
| PCT/US2021/051142 WO2022061247A2 (en) | 2020-09-18 | 2021-09-20 | Constructs and uses thereof for efficient and specific genome editing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3193089A1 true CA3193089A1 (en) | 2022-03-24 |
Family
ID=80777856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3193089A Pending CA3193089A1 (en) | 2020-09-18 | 2021-09-20 | Constructs and uses thereof for efficient and specific genome editing |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US12571006B2 (https=) |
| EP (1) | EP4214311A2 (https=) |
| JP (1) | JP2023543351A (https=) |
| AU (1) | AU2021343526A1 (https=) |
| CA (1) | CA3193089A1 (https=) |
| WO (1) | WO2022061247A2 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| WO2022147157A1 (en) * | 2020-12-31 | 2022-07-07 | Gigamune, Inc. | Novel nucleic acid-guided nucleases |
| AU2022227650A1 (en) * | 2021-02-25 | 2023-10-12 | Celyntra Therapeutics Sa | Compositions and methods for targeting, editing, or modifying genes |
| WO2024042168A1 (en) * | 2022-08-26 | 2024-02-29 | UCB Biopharma SRL | Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases |
| IL321270A (en) * | 2022-12-13 | 2025-08-01 | Bayer Ag | Engineered RNA-type programmable endonucleases and their uses |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| CA2969619A1 (en) | 2014-12-03 | 2016-06-09 | Agilent Technologies, Inc. | Guide rna with chemical modifications |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| CN108690845B (zh) * | 2017-04-10 | 2021-04-27 | 中国科学院动物研究所 | 基因组编辑系统和方法 |
| US20210139889A1 (en) * | 2017-06-19 | 2021-05-13 | Yale University | Compositions and Methods for Multiplexed Genome Editing and Screening |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| JP7689949B2 (ja) | 2019-10-03 | 2025-06-09 | アーティサン ディベロップメント ラブズ インコーポレイテッド | 操作されたデュアルガイド核酸を有するｃｒｉｓｐｒシステム |
| WO2021074191A1 (en) | 2019-10-14 | 2021-04-22 | KWS SAAT SE & Co. KGaA | Mad7 nuclease in plants and expanding its pam recognition capability |
| US11453907B2 (en) * | 2020-03-23 | 2022-09-27 | The Broad Institute, Inc. | Crispr effector system based coronavirus diagnostics |
-
2021
- 2021-09-20 CA CA3193089A patent/CA3193089A1/en active Pending
- 2021-09-20 JP JP2023542827A patent/JP2023543351A/ja active Pending
- 2021-09-20 WO PCT/US2021/051142 patent/WO2022061247A2/en not_active Ceased
- 2021-09-20 AU AU2021343526A patent/AU2021343526A1/en active Pending
- 2021-09-20 EP EP21870385.8A patent/EP4214311A2/en not_active Withdrawn
- 2021-09-20 US US18/027,054 patent/US12571006B2/en active Active
-
2023
- 2023-05-02 US US18/142,413 patent/US20240011054A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2021343526A1 (en) | 2023-05-25 |
| US12571006B2 (en) | 2026-03-10 |
| AU2021343526A9 (en) | 2024-10-03 |
| EP4214311A2 (en) | 2023-07-26 |
| US20240011054A1 (en) | 2024-01-11 |
| JP2023543351A (ja) | 2023-10-13 |
| WO2022061247A2 (en) | 2022-03-24 |
| US20230323405A1 (en) | 2023-10-12 |
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