CA3177368A1 - Dispositifs et procedes de sequencage - Google Patents

Dispositifs et procedes de sequencage

Info

Publication number
CA3177368A1
CA3177368A1 CA3177368A CA3177368A CA3177368A1 CA 3177368 A1 CA3177368 A1 CA 3177368A1 CA 3177368 A CA3177368 A CA 3177368A CA 3177368 A CA3177368 A CA 3177368A CA 3177368 A1 CA3177368 A1 CA 3177368A1
Authority
CA
Canada
Prior art keywords
cartridge
sample
target molecules
lysis
capture probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3177368A
Other languages
English (en)
Inventor
Jonathan M. Rothberg
John H. Leamon
Jonathan C. Schultz
Michele Millham
Caixia Lv
Haidong Huang
Roger Nani
Omer AD
Brian Reed
Matthew Dyer
Robert E. Boer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of CA3177368A1 publication Critical patent/CA3177368A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/06—Separation of liquids from each other by electricity
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/159—Reduction of complexity, e.g. amplification of subsets, removing duplicated genomic regions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Thermal Sciences (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne des procédés et des dispositifs pour préparer des molécules cibles (par exemple, des acides nucléiques cibles ou des protéines cibles) à partir d'un échantillon biologique. Dans certains modes de réalisation, des procédés et des dispositifs impliquent la lyse d'échantillon, la fragmentation d'échantillon, l'enrichissement de la molécule(s) cible(s), et/ou la fonctionnalisation de la molécule(s) cible(s).
CA3177368A 2020-04-22 2021-04-21 Dispositifs et procedes de sequencage Pending CA3177368A1 (fr)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US202063014093P 2020-04-22 2020-04-22
US202063014071P 2020-04-22 2020-04-22
US202063014106P 2020-04-22 2020-04-22
US202063014087P 2020-04-22 2020-04-22
US63/014,087 2020-04-22
US63/014,071 2020-04-22
US63/014,093 2020-04-22
US63/014,106 2020-04-22
US202063041206P 2020-06-19 2020-06-19
US63/041,206 2020-06-19
US202163139348P 2021-01-20 2021-01-20
US202163139339P 2021-01-20 2021-01-20
US202163139346P 2021-01-20 2021-01-20
US202163139343P 2021-01-20 2021-01-20
US63/139,348 2021-01-20
US63/139,346 2021-01-20
US63/139,343 2021-01-20
US63/139,339 2021-01-20
PCT/US2021/028471 WO2021216763A1 (fr) 2020-04-22 2021-04-21 Dispositifs et procédés de séquençage

Publications (1)

Publication Number Publication Date
CA3177368A1 true CA3177368A1 (fr) 2021-10-28

Family

ID=78269941

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3177368A Pending CA3177368A1 (fr) 2020-04-22 2021-04-21 Dispositifs et procedes de sequencage

Country Status (7)

Country Link
EP (1) EP4132674A4 (fr)
JP (1) JP2023523592A (fr)
KR (1) KR20220159496A (fr)
CN (1) CN115916365A (fr)
AU (1) AU2021261356A1 (fr)
CA (1) CA3177368A1 (fr)
WO (1) WO2021216763A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3731959A4 (fr) 2017-12-29 2021-10-13 Clear Labs Inc. Dispositif automatisé d'amorçage et de chargement de bibliothèque

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020022261A1 (en) * 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US6818185B1 (en) * 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
US7541166B2 (en) * 2003-09-19 2009-06-02 Microfluidic Systems, Inc. Sonication to selectively lyse different cell types
EP2072133A1 (fr) * 2007-12-20 2009-06-24 Koninklijke Philips Electronics N.V. Dispositif à plusieurs compartiments doté de particules magnétiques
KR20110030415A (ko) * 2008-01-22 2011-03-23 인터젠엑스 인크. 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9506934B2 (en) * 2013-04-29 2016-11-29 Honeywell International Inc. Polymer test cartridge mixer for cell lysis
WO2016065300A1 (fr) * 2014-10-24 2016-04-28 Eshoo Mark W Cartouche microfluidique
EP3548603A4 (fr) * 2016-11-29 2020-10-28 S2 Genomics, Inc. Procédé et appareil pour traiter des échantillons de tissu

Also Published As

Publication number Publication date
EP4132674A4 (fr) 2024-05-29
WO2021216763A1 (fr) 2021-10-28
CN115916365A (zh) 2023-04-04
AU2021261356A1 (en) 2022-12-15
JP2023523592A (ja) 2023-06-06
EP4132674A1 (fr) 2023-02-15
KR20220159496A (ko) 2022-12-02

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Legal Events

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Effective date: 20220927

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EEER Examination request

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EEER Examination request

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