CA3152679A1 - Atypical split inteins and uses thereof - Google Patents

Atypical split inteins and uses thereof Download PDF

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Publication number
CA3152679A1
CA3152679A1 CA3152679A CA3152679A CA3152679A1 CA 3152679 A1 CA3152679 A1 CA 3152679A1 CA 3152679 A CA3152679 A CA 3152679A CA 3152679 A CA3152679 A CA 3152679A CA 3152679 A1 CA3152679 A1 CA 3152679A1
Authority
CA
Canada
Prior art keywords
fragment
seq
interest
split intein
terminus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3152679A
Other languages
English (en)
French (fr)
Inventor
Tom W. Muir
Adam Stevens
Josef GRAMESPACHER
David Cowburn
Giridhar SEKAR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Albert Einstein College of Medicine
Princeton University
Original Assignee
Albert Einstein College of Medicine
Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Albert Einstein College of Medicine, Princeton University filed Critical Albert Einstein College of Medicine
Publication of CA3152679A1 publication Critical patent/CA3152679A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C07K1/026—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution by fragment condensation in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3152679A 2019-08-28 2019-08-28 Atypical split inteins and uses thereof Pending CA3152679A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2019/048508 WO2021040703A1 (en) 2019-08-28 2019-08-28 Atypical split inteins and uses thereof

Publications (1)

Publication Number Publication Date
CA3152679A1 true CA3152679A1 (en) 2021-03-04

Family

ID=74684576

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3152679A Pending CA3152679A1 (en) 2019-08-28 2019-08-28 Atypical split inteins and uses thereof

Country Status (5)

Country Link
US (1) US20220275027A1 (https=)
JP (2) JP7579844B2 (https=)
AU (1) AU2019463636A1 (https=)
CA (1) CA3152679A1 (https=)
WO (1) WO2021040703A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120569628A (zh) 2023-01-10 2025-08-29 核蛋白有限公司 蛋白聚集测定

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6562576B2 (en) * 2001-01-04 2003-05-13 Myriad Genetics, Inc. Yeast two-hybrid system and use thereof
EP3431497B1 (en) * 2012-06-27 2022-07-27 The Trustees of Princeton University Split inteins, conjugates and uses thereof
US10087213B2 (en) * 2013-01-11 2018-10-02 The Texas A&M University System Intein mediated purification of protein
EP2883953A1 (en) * 2013-12-12 2015-06-17 Westfälische Wilhelms-Universität Münster An atypical naturally split intein engineered for highly efficient protein modification
PT3408292T (pt) * 2016-01-29 2023-07-19 Univ Princeton Inteínas divididas com excecional atividade de splicing
EP3885440A1 (en) * 2020-03-26 2021-09-29 Splicebio, S.L. Split inteins and their uses

Also Published As

Publication number Publication date
US20220275027A1 (en) 2022-09-01
JP7579844B2 (ja) 2024-11-08
JP2024156675A (ja) 2024-11-06
JP2022552598A (ja) 2022-12-19
AU2019463636A1 (en) 2022-03-17
WO2021040703A1 (en) 2021-03-04

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