CA3129656A1 - Generation of human pluripotent stem cell derived artificial tissue structures without three dimensional matrices - Google Patents

Generation of human pluripotent stem cell derived artificial tissue structures without three dimensional matrices Download PDF

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Publication number
CA3129656A1
CA3129656A1 CA3129656A CA3129656A CA3129656A1 CA 3129656 A1 CA3129656 A1 CA 3129656A1 CA 3129656 A CA3129656 A CA 3129656A CA 3129656 A CA3129656 A CA 3129656A CA 3129656 A1 CA3129656 A1 CA 3129656A1
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mpa
medium
cells
cellulose
differentiation
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CA3129656A
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English (en)
French (fr)
Inventor
Kristin BECKER
Dominik Eckardt
Andreas Bosio
Sebastian Knobel
David Joel Agorku
Olaf Thorsten HARDT
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Miltenyi Biotec GmbH
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Miltenyi Biotec GmbH
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Publication of CA3129656A1 publication Critical patent/CA3129656A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/78—Cellulose

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3129656A 2019-02-11 2020-02-10 Generation of human pluripotent stem cell derived artificial tissue structures without three dimensional matrices Pending CA3129656A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19156450.9 2019-02-11
EP19156450 2019-02-11
PCT/EP2020/053237 WO2020165059A1 (en) 2019-02-11 2020-02-10 Generation of human pluripotent stem cell derived artificial tissue structures without three dimensional matrices

Publications (1)

Publication Number Publication Date
CA3129656A1 true CA3129656A1 (en) 2020-08-20

Family

ID=65408960

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3129656A Pending CA3129656A1 (en) 2019-02-11 2020-02-10 Generation of human pluripotent stem cell derived artificial tissue structures without three dimensional matrices

Country Status (5)

Country Link
US (1) US20220145247A1 (https=)
EP (1) EP3924468A1 (https=)
JP (1) JP7541986B2 (https=)
CA (1) CA3129656A1 (https=)
WO (1) WO2020165059A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240279602A1 (en) * 2021-06-17 2024-08-22 Kyoto University Method for producing cerebral cortical cell preparation derived from human pluripotent stem cells

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4206320A4 (en) * 2020-08-21 2025-02-12 JSR Corporation METHOD FOR CULTIVATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS, CULTURE OF HUMAN INDUCED PLURIPOTENT STEM CELLS AND METHOD FOR THE PRODUCTION OF CEREBRAL ORGANOIDS
CN112685978B (zh) * 2021-03-11 2021-06-08 中国空气动力研究与发展中心计算空气动力研究所 一种适用于五次样条重构格式的自适应人工粘性控制方法
EP4544025A1 (en) * 2022-06-21 2025-04-30 Institut National de la Santé et de la Recherche Médicale A method for producing a bioengineered mammal induced pluripotent stem cell-derived cardiac organoid

Family Cites Families (12)

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Publication number Priority date Publication date Assignee Title
JPH0779772A (ja) * 1993-09-13 1995-03-28 W R Grace & Co 細胞培養液及びその培養液を用いたスフェロイドの製造方法
US6632661B2 (en) * 2000-12-07 2003-10-14 3M Innovative Properties Company Self-spreading microbiological culture device
MXPA04005010A (es) 2001-11-26 2005-04-08 Advanced Cell Tech Inc METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS.
JPWO2009148170A1 (ja) 2008-06-06 2011-11-04 独立行政法人理化学研究所 幹細胞の培養方法
JP5787370B2 (ja) 2009-11-05 2015-09-30 国立研究開発法人理化学研究所 幹細胞の分化誘導方法
EP2743345A1 (en) 2012-12-13 2014-06-18 IMBA-Institut für Molekulare Biotechnologie GmbH Three dimensional heterogeneously differentiated tissue culture
KR102539240B1 (ko) * 2014-01-23 2023-06-01 닛산 가가쿠 가부시키가이샤 배양 배지 조성물
CN107427537A (zh) * 2015-03-03 2017-12-01 哈佛学院院长及董事 产生功能性人体组织的方法
US11066660B2 (en) * 2015-05-14 2021-07-20 Public University Corporation Yokohama City University Technique for aggregating macromolecules together with cells
WO2017160234A1 (en) * 2016-03-14 2017-09-21 Agency For Science, Technology And Research Generation of midbrain-specific organoids from human pluripotent stem cells
EP3296018A1 (en) * 2016-09-19 2018-03-21 Ecole Polytechnique Fédérale de Lausanne (EPFL) Organoid arrays
ES2903187T3 (es) * 2017-06-13 2022-03-31 Georg August Univ Goettingen Stiftung Oeffentlichen Rechts Univsmedizin Procedimientos de producción de organoides neuronales de bioingeniería (BENO) y sus usos

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240279602A1 (en) * 2021-06-17 2024-08-22 Kyoto University Method for producing cerebral cortical cell preparation derived from human pluripotent stem cells

Also Published As

Publication number Publication date
WO2020165059A1 (en) 2020-08-20
JP2022519376A (ja) 2022-03-23
US20220145247A1 (en) 2022-05-12
EP3924468A1 (en) 2021-12-22
JP7541986B2 (ja) 2024-08-29

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