CA3042233A1 - Plants with improved growth - Google Patents
Plants with improved growth Download PDFInfo
- Publication number
- CA3042233A1 CA3042233A1 CA3042233A CA3042233A CA3042233A1 CA 3042233 A1 CA3042233 A1 CA 3042233A1 CA 3042233 A CA3042233 A CA 3042233A CA 3042233 A CA3042233 A CA 3042233A CA 3042233 A1 CA3042233 A1 CA 3042233A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- promoter
- plant
- coding sequence
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000012010 growth Effects 0.000 title description 85
- 230000001976 improved effect Effects 0.000 title description 15
- 108091026890 Coding region Proteins 0.000 claims abstract description 80
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 70
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 66
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 66
- 238000000034 method Methods 0.000 claims abstract description 42
- 101001066230 Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) Cytochrome P450 monooygenase 2 Proteins 0.000 claims abstract description 32
- 230000000442 meristematic effect Effects 0.000 claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims description 278
- 230000014509 gene expression Effects 0.000 claims description 104
- 241000219000 Populus Species 0.000 claims description 54
- 230000000694 effects Effects 0.000 claims description 53
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 49
- 238000011144 upstream manufacturing Methods 0.000 claims description 35
- 238000013518 transcription Methods 0.000 claims description 34
- 230000035897 transcription Effects 0.000 claims description 34
- 239000002023 wood Substances 0.000 claims description 33
- 230000000977 initiatory effect Effects 0.000 claims description 28
- 101100012019 Mus musculus Etv4 gene Proteins 0.000 claims description 27
- 230000001965 increasing effect Effects 0.000 claims description 26
- 241001233195 Eucalyptus grandis Species 0.000 claims description 23
- 241000894007 species Species 0.000 claims description 23
- 108091081024 Start codon Proteins 0.000 claims description 20
- 239000002773 nucleotide Substances 0.000 claims description 20
- 125000003729 nucleotide group Chemical group 0.000 claims description 20
- 239000002028 Biomass Substances 0.000 claims description 19
- 241000218976 Populus trichocarpa Species 0.000 claims description 19
- 238000004519 manufacturing process Methods 0.000 claims description 19
- 241000219195 Arabidopsis thaliana Species 0.000 claims description 17
- 230000001105 regulatory effect Effects 0.000 claims description 17
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 12
- 230000002792 vascular Effects 0.000 claims description 11
- 239000011121 hardwood Substances 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 6
- 230000001172 regenerating effect Effects 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 244000038559 crop plants Species 0.000 claims description 4
- 241000335053 Beta vulgaris Species 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 244000299507 Gossypium hirsutum Species 0.000 claims description 3
- 240000005979 Hordeum vulgare Species 0.000 claims description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 235000007238 Secale cereale Nutrition 0.000 claims description 3
- 244000082988 Secale cereale Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 244000098338 Triticum aestivum Species 0.000 claims description 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 3
- 235000009973 maize Nutrition 0.000 claims description 3
- 235000000832 Ayote Nutrition 0.000 claims description 2
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 2
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 240000004244 Cucurbita moschata Species 0.000 claims description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 claims description 2
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 claims description 2
- 240000003183 Manihot esculenta Species 0.000 claims description 2
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 2
- 240000000111 Saccharum officinarum Species 0.000 claims description 2
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 239000004459 forage Substances 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 235000015136 pumpkin Nutrition 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 230000009105 vegetative growth Effects 0.000 claims description 2
- 244000166124 Eucalyptus globulus Species 0.000 claims 1
- 235000012015 potatoes Nutrition 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 description 178
- 108700004756 oxidizing) 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating gibberellin Proteins 0.000 description 64
- 230000009261 transgenic effect Effects 0.000 description 62
- 241000219927 Eucalyptus Species 0.000 description 46
- 241000183024 Populus tremula Species 0.000 description 42
- 210000004027 cell Anatomy 0.000 description 40
- 239000012634 fragment Substances 0.000 description 30
- 229920001184 polypeptide Polymers 0.000 description 26
- 102000004196 processed proteins & peptides Human genes 0.000 description 26
- 108090000765 processed proteins & peptides Proteins 0.000 description 26
- 108020004511 Recombinant DNA Proteins 0.000 description 24
- 230000009466 transformation Effects 0.000 description 21
- 238000004458 analytical method Methods 0.000 description 19
- 230000002018 overexpression Effects 0.000 description 19
- 239000013598 vector Substances 0.000 description 18
- 108020004414 DNA Proteins 0.000 description 16
- 230000006870 function Effects 0.000 description 15
- 241000219194 Arabidopsis Species 0.000 description 14
- 230000015572 biosynthetic process Effects 0.000 description 14
- 108091028043 Nucleic acid sequence Proteins 0.000 description 13
- 238000010276 construction Methods 0.000 description 13
- 230000008635 plant growth Effects 0.000 description 12
- 229930191978 Gibberellin Natural products 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
- 239000003448 gibberellin Substances 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 230000006798 recombination Effects 0.000 description 10
- 238000005215 recombination Methods 0.000 description 10
- 101100059375 Arabidopsis thaliana CYCD3-1 gene Proteins 0.000 description 9
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 9
- 238000010367 cloning Methods 0.000 description 9
- 238000013081 phylogenetic analysis Methods 0.000 description 9
- 235000018102 proteins Nutrition 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 241000217825 Populus tremula x Populus tremuloides Species 0.000 description 8
- 230000001276 controlling effect Effects 0.000 description 8
- 238000011161 development Methods 0.000 description 8
- 230000018109 developmental process Effects 0.000 description 8
- 210000000056 organ Anatomy 0.000 description 8
- 238000003786 synthesis reaction Methods 0.000 description 8
- 241000589158 Agrobacterium Species 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 108090000854 Oxidoreductases Proteins 0.000 description 7
- 230000002411 adverse Effects 0.000 description 7
- 230000002520 cambial effect Effects 0.000 description 7
- 241000124033 Salix Species 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical group C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 230000001404 mediated effect Effects 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 240000008395 Elaeocarpus angustifolius Species 0.000 description 5
- 244000004281 Eucalyptus maculata Species 0.000 description 5
- 229940024606 amino acid Drugs 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 5
- 230000032823 cell division Effects 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000008929 regeneration Effects 0.000 description 5
- 238000011069 regeneration method Methods 0.000 description 5
- 238000007619 statistical method Methods 0.000 description 5
- 241000208140 Acer Species 0.000 description 4
- 241000726768 Carpinus Species 0.000 description 4
- 241000723418 Carya Species 0.000 description 4
- 241001070941 Castanea Species 0.000 description 4
- 235000014036 Castanea Nutrition 0.000 description 4
- 241000218631 Coniferophyta Species 0.000 description 4
- -1 Gibberellins Gibberellins Chemical class 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 241000758789 Juglans Species 0.000 description 4
- 240000007472 Leucaena leucocephala Species 0.000 description 4
- 235000010643 Leucaena leucocephala Nutrition 0.000 description 4
- 241000208682 Liquidambar Species 0.000 description 4
- 102000004316 Oxidoreductases Human genes 0.000 description 4
- 241000219492 Quercus Species 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 238000002493 microarray Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 230000034655 secondary growth Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 240000004731 Acer pseudoplatanus Species 0.000 description 3
- 235000002754 Acer pseudoplatanus Nutrition 0.000 description 3
- 235000018185 Betula X alpestris Nutrition 0.000 description 3
- 235000018212 Betula X uliginosa Nutrition 0.000 description 3
- 241000219495 Betulaceae Species 0.000 description 3
- 230000006820 DNA synthesis Effects 0.000 description 3
- 240000000731 Fagus sylvatica Species 0.000 description 3
- 235000010099 Fagus sylvatica Nutrition 0.000 description 3
- 235000009496 Juglans regia Nutrition 0.000 description 3
- 235000006552 Liquidambar styraciflua Nutrition 0.000 description 3
- 235000006485 Platanus occidentalis Nutrition 0.000 description 3
- 241000218998 Salicaceae Species 0.000 description 3
- 244000186561 Swietenia macrophylla Species 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 3
- 239000004062 cytokinin Substances 0.000 description 3
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000030279 gene silencing Effects 0.000 description 3
- 238000012226 gene silencing method Methods 0.000 description 3
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 3
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 3
- 230000001744 histochemical effect Effects 0.000 description 3
- 230000001771 impaired effect Effects 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 230000004983 pleiotropic effect Effects 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000004904 shortening Methods 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 210000000130 stem cell Anatomy 0.000 description 3
- 230000002123 temporal effect Effects 0.000 description 3
- 235000020234 walnut Nutrition 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241001133760 Acoelorraphe Species 0.000 description 2
- 235000003840 Amygdalus nana Nutrition 0.000 description 2
- 244000296825 Amygdalus nana Species 0.000 description 2
- 101001025764 Arabidopsis thaliana Gibberellin 20 oxidase 1 Proteins 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 241000219198 Brassica Species 0.000 description 2
- 235000011331 Brassica Nutrition 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 244000197813 Camelina sativa Species 0.000 description 2
- 241000207199 Citrus Species 0.000 description 2
- 101710201891 Gibberellin 20 oxidase 1 Proteins 0.000 description 2
- 235000011201 Ginkgo Nutrition 0.000 description 2
- 235000008100 Ginkgo biloba Nutrition 0.000 description 2
- 244000194101 Ginkgo biloba Species 0.000 description 2
- 102000053187 Glucuronidase Human genes 0.000 description 2
- 108010060309 Glucuronidase Proteins 0.000 description 2
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 241000218922 Magnoliophyta Species 0.000 description 2
- 241000220225 Malus Species 0.000 description 2
- 241000219823 Medicago Species 0.000 description 2
- 108700005084 Multigene Family Proteins 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 101100121112 Oryza sativa subsp. indica 20ox2 gene Proteins 0.000 description 2
- 101100121113 Oryza sativa subsp. japonica GA20OX2 gene Proteins 0.000 description 2
- 102000001253 Protein Kinase Human genes 0.000 description 2
- 235000011432 Prunus Nutrition 0.000 description 2
- 241000220324 Pyrus Species 0.000 description 2
- 238000003559 RNA-seq method Methods 0.000 description 2
- 108700008625 Reporter Genes Proteins 0.000 description 2
- 101150065399 WOX4 gene Proteins 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000002551 biofuel Substances 0.000 description 2
- 239000004566 building material Substances 0.000 description 2
- 235000020971 citrus fruits Nutrition 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000001739 density measurement Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 229930004069 diterpene Natural products 0.000 description 2
- 125000000567 diterpene group Chemical group 0.000 description 2
- 230000005059 dormancy Effects 0.000 description 2
- 238000010195 expression analysis Methods 0.000 description 2
- 238000012239 gene modification Methods 0.000 description 2
- 238000003208 gene overexpression Methods 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000003375 plant hormone Substances 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 108060006633 protein kinase Proteins 0.000 description 2
- 235000014774 prunus Nutrition 0.000 description 2
- 238000004537 pulping Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000001020 rhythmical effect Effects 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 230000003442 weekly effect Effects 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 1
- 239000001707 (E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-ol Substances 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 101150084750 1 gene Proteins 0.000 description 1
- 101150090724 3 gene Proteins 0.000 description 1
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- 241000218642 Abies Species 0.000 description 1
- 241000219068 Actinidia Species 0.000 description 1
- 235000009436 Actinidia deliciosa Nutrition 0.000 description 1
- 244000298697 Actinidia deliciosa Species 0.000 description 1
- 235000003310 Adansonia Nutrition 0.000 description 1
- 241000982340 Adansonia Species 0.000 description 1
- 241000219496 Alnus Species 0.000 description 1
- 108700024648 Arabidopsis WOX4 Proteins 0.000 description 1
- 108010073142 Arabidopsis gibberellin 20 oxidase 1 Proteins 0.000 description 1
- 101100000339 Arabidopsis thaliana ABCG11 gene Proteins 0.000 description 1
- 101100011901 Arabidopsis thaliana ERL1 gene Proteins 0.000 description 1
- 101100011904 Arabidopsis thaliana ERL2 gene Proteins 0.000 description 1
- 101100411929 Arabidopsis thaliana RBCS-1A gene Proteins 0.000 description 1
- 101100411930 Arabidopsis thaliana RBCS-1B gene Proteins 0.000 description 1
- 101100467814 Arabidopsis thaliana RBCS-2B gene Proteins 0.000 description 1
- 101100247536 Arabidopsis thaliana RBCS-3B gene Proteins 0.000 description 1
- 241000722818 Aralia Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 235000005781 Avena Nutrition 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 235000021533 Beta vulgaris Nutrition 0.000 description 1
- 235000003932 Betula Nutrition 0.000 description 1
- 241000219429 Betula Species 0.000 description 1
- 241000743774 Brachypodium Species 0.000 description 1
- 241000743776 Brachypodium distachyon Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000008100 Brassica rapa Species 0.000 description 1
- 235000011292 Brassica rapa Nutrition 0.000 description 1
- 235000016401 Camelina Nutrition 0.000 description 1
- 235000014595 Camelina sativa Nutrition 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000701489 Cauliflower mosaic virus Species 0.000 description 1
- 241000218645 Cedrus Species 0.000 description 1
- 108010074879 Cinnamoyl-CoA reductase Proteins 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241001415288 Coccidae Species 0.000 description 1
- 235000010071 Cucumis prophetarum Nutrition 0.000 description 1
- 244000024469 Cucumis prophetarum Species 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 241000218691 Cupressaceae Species 0.000 description 1
- 241000723198 Cupressus Species 0.000 description 1
- 102000016736 Cyclin Human genes 0.000 description 1
- 108050006400 Cyclin Proteins 0.000 description 1
- FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 1
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 108700021058 Dynamin Proteins 0.000 description 1
- 101150044894 ER gene Proteins 0.000 description 1
- 241001070947 Fagus Species 0.000 description 1
- 241000218218 Ficus <angiosperm> Species 0.000 description 1
- 244000286663 Ficus elastica Species 0.000 description 1
- 241001536358 Fraxinus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000013446 GTP Phosphohydrolases Human genes 0.000 description 1
- 108091006109 GTPases Proteins 0.000 description 1
- 101150112014 Gapdh gene Proteins 0.000 description 1
- 239000005980 Gibberellic acid Substances 0.000 description 1
- 101710201886 Gibberellin 20 oxidase 3 Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000459819 Gymnodinium cucumis Species 0.000 description 1
- 241000209219 Hordeum Species 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 235000013757 Juglans Nutrition 0.000 description 1
- 241000721662 Juniperus Species 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 241000218652 Larix Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 108010006444 Leucine-Rich Repeat Proteins Proteins 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 235000006550 Liquidambar Nutrition 0.000 description 1
- 241000486288 Mimulus <crab> Species 0.000 description 1
- 244000179886 Moringa oleifera Species 0.000 description 1
- 235000011347 Moringa oleifera Nutrition 0.000 description 1
- 241000218213 Morus <angiosperm> Species 0.000 description 1
- 102000006833 Multifunctional Enzymes Human genes 0.000 description 1
- 108010047290 Multifunctional Enzymes Proteins 0.000 description 1
- 241000219926 Myrtaceae Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 206010067482 No adverse event Diseases 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101150033653 PGS1 gene Proteins 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- BLUHKGOSFDHHGX-UHFFFAOYSA-N Phytol Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)C=CO BLUHKGOSFDHHGX-UHFFFAOYSA-N 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 241000218641 Pinaceae Species 0.000 description 1
- 241000186704 Pinales Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 235000000405 Pinus densiflora Nutrition 0.000 description 1
- 240000008670 Pinus densiflora Species 0.000 description 1
- 240000008334 Pisonia alba Species 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 241000209466 Platanus Species 0.000 description 1
- 241001657474 Populus alba x Populus glandulosa Species 0.000 description 1
- 235000011263 Populus tremuloides Nutrition 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 description 1
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 description 1
- 241001290151 Prunus avium subsp. avium Species 0.000 description 1
- 241000218683 Pseudotsuga Species 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 241001116459 Sequoia Species 0.000 description 1
- 241000422847 Sequoiadendron Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 241000159144 Swietenia Species 0.000 description 1
- 241001116495 Taxaceae Species 0.000 description 1
- 241001116500 Taxus Species 0.000 description 1
- HNZBNQYXWOLKBA-UHFFFAOYSA-N Tetrahydrofarnesol Natural products CC(C)CCCC(C)CCCC(C)=CCO HNZBNQYXWOLKBA-UHFFFAOYSA-N 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 241000949476 Toona Species 0.000 description 1
- 108700009124 Transcription Initiation Site Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 240000003021 Tsuga heterophylla Species 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- BOTWFXYSPFMFNR-OALUTQOASA-N all-rac-phytol Natural products CC(C)CCC[C@H](C)CCC[C@H](C)CCCC(C)=CCO BOTWFXYSPFMFNR-OALUTQOASA-N 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229940009098 aspartate Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 238000002247 constant time method Methods 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000012202 endocytosis Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000011990 functional testing Methods 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 230000004545 gene duplication Effects 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 229940049906 glutamate Drugs 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 229960002743 glutamine Drugs 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 235000021384 green leafy vegetables Nutrition 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 101150054900 gus gene Proteins 0.000 description 1
- 108091008147 housekeeping proteins Proteins 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 230000011890 leaf development Effects 0.000 description 1
- 210000004901 leucine-rich repeat Anatomy 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 238000002705 metabolomic analysis Methods 0.000 description 1
- 230000001431 metabolomic effect Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 235000006109 methionine Nutrition 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 101150098295 pel1 gene Proteins 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- BOTWFXYSPFMFNR-PYDDKJGSSA-N phytol Chemical compound CC(C)CCC[C@@H](C)CCC[C@@H](C)CCC\C(C)=C\CO BOTWFXYSPFMFNR-PYDDKJGSSA-N 0.000 description 1
- 239000001739 pinus spp. Substances 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000003762 quantitative reverse transcription PCR Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 1
- 235000016491 selenocysteine Nutrition 0.000 description 1
- 229940055619 selenocysteine Drugs 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 235000004400 serine Nutrition 0.000 description 1
- UQDJGEHQDNVPGU-UHFFFAOYSA-N serine phosphoethanolamine Chemical compound [NH3+]CCOP([O-])(=O)OCC([NH3+])C([O-])=O UQDJGEHQDNVPGU-UHFFFAOYSA-N 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- 230000005082 stem growth Effects 0.000 description 1
- 230000036435 stunted growth Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 235000008521 threonine Nutrition 0.000 description 1
- 230000009772 tissue formation Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8229—Meristem-specific, e.g. nodal, apical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8291—Hormone-influenced development
- C12N15/8297—Gibberellins; GA3
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE1651431 | 2016-10-31 | ||
| SE1651431-7 | 2016-10-31 | ||
| PCT/SE2017/051065 WO2018080389A1 (en) | 2016-10-31 | 2017-10-30 | Plants with improved growth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3042233A1 true CA3042233A1 (en) | 2018-05-03 |
Family
ID=62023902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3042233A Pending CA3042233A1 (en) | 2016-10-31 | 2017-10-30 | Plants with improved growth |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20230151377A1 (es) |
| EP (1) | EP3532621A4 (es) |
| CA (1) | CA3042233A1 (es) |
| CL (1) | CL2019000873A1 (es) |
| UY (1) | UY37453A (es) |
| WO (1) | WO2018080389A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11608506B2 (en) | 2018-06-26 | 2023-03-21 | Regents Of The University Of Minnesota | Delivery of developmental regulators to plants for the induction of meristematic tissue with genetic alterations |
| EP3975701A4 (en) * | 2019-05-29 | 2023-08-09 | Monsanto Technology LLC | METHODS AND COMPOSITIONS FOR GENERATE SMALL DOMINANT ALLELE BY GENOME EDITING |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9311147D0 (en) * | 1993-05-28 | 1993-07-14 | Long Ashton Research Station | Regulation of plant growth |
| US6723897B2 (en) * | 1998-08-10 | 2004-04-20 | Monsanto Technology, Llc | Methods for controlling gibberellin levels |
| CA2263067A1 (en) * | 1999-02-26 | 2000-08-26 | The Australian National University | Method of modifying plant morphology, biochemistry and physiology |
| SE0000751D0 (sv) * | 2000-03-07 | 2000-03-07 | Swetree Genomics Ab | Transgenic trees and methods for their production |
| PT1261726E (pt) * | 2000-03-07 | 2006-09-29 | Swetree Technologies Ab | Arvores transgenicas apresentando um aumento na producao de biomassa e no comprimento de fibras do xilema, e metodos para a sua producao |
| KR20010090954A (ko) * | 2000-04-08 | 2001-10-22 | 우종일 | 수박의 종자 발생시 종피특이적으로 유전자의 발현을조절하는 Ｃｖ２０ｏｘ유전자 프로모터 |
| FR2840321A1 (fr) * | 2002-05-29 | 2003-12-05 | Genoplante Valor | Promoteur specifique des tissus vegetaux formant des parois secondaires |
| US7507875B2 (en) * | 2003-06-06 | 2009-03-24 | Arborgen, Llc | Transcription factors |
| GB0422977D0 (en) * | 2004-10-15 | 2004-11-17 | Swetree Technologies Ab | Methods for improving tree growth and wood properties |
| US20070283455A1 (en) * | 2006-05-31 | 2007-12-06 | Gray Dennis J | Genetic Transformation of Grapevines |
| WO2010138971A1 (en) * | 2009-05-29 | 2010-12-02 | Edenspace Systems Corporation | Plant gene regulatory elements |
| US20130117890A1 (en) * | 2009-11-25 | 2013-05-09 | Scg Paper Public Company Limited | Processes for accelerating plant growth and increasing cellulose yield |
| KR101710795B1 (ko) * | 2014-08-20 | 2017-03-02 | 경희대학교 산학협력단 | 바이오매스가 증가된 형질전환 식물 및 이의 제조방법 |
| WO2016108750A1 (en) * | 2014-12-29 | 2016-07-07 | Swetree Technologies Ab | Woody plants having improved growth properties |
| WO2019112509A1 (en) * | 2017-12-04 | 2019-06-13 | Swetree Technologies Ab | Plants with improved growth |
-
2017
- 2017-10-26 UY UY0001037453A patent/UY37453A/es unknown
- 2017-10-30 US US16/346,487 patent/US20230151377A1/en active Pending
- 2017-10-30 EP EP17865309.3A patent/EP3532621A4/en active Pending
- 2017-10-30 WO PCT/SE2017/051065 patent/WO2018080389A1/en unknown
- 2017-10-30 CA CA3042233A patent/CA3042233A1/en active Pending
-
2019
- 2019-04-01 CL CL2019000873A patent/CL2019000873A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20230151377A1 (en) | 2023-05-18 |
| EP3532621A4 (en) | 2020-08-19 |
| EP3532621A1 (en) | 2019-09-04 |
| UY37453A (es) | 2018-05-31 |
| CL2019000873A1 (es) | 2019-07-26 |
| WO2018080389A1 (en) | 2018-05-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wuddineh et al. | Identification and overexpression of gibberellin 2‐oxidase (GA 2ox) in switchgrass (P anicum virgatum L.) for improved plant architecture and reduced biomass recalcitrance | |
| US8088975B2 (en) | Phenylpropanoid related regulatory protein-regulatory region associations | |
| AU2014203225B2 (en) | Woody plants having improved growth characteristics and method for making the same using transcription factors | |
| Ji et al. | Isolation and characterisation of a dwarf rice mutant exhibiting defective gibberellins biosynthesis | |
| BRPI0613141A2 (pt) | polinucleotìdios isolados, construção de dna, célula de planta, planta transgênica, madeira e polpa de madeira | |
| US7897839B2 (en) | Methods for modifying plant characteristics | |
| US20230151377A1 (en) | Plants with improved growth | |
| US20210403932A1 (en) | Plants with Improved Growth | |
| US20230279419A1 (en) | Enhancement of productivity in c3 plants | |
| AU2013230864B2 (en) | Plants having improved growth properties | |
| Quan et al. | Molecular cloning and expression analysis of the MTN gene during adventitious root development in IBA-induced tetraploid black locust | |
| US9758790B2 (en) | Modulating the level of components within plants | |
| US11441152B2 (en) | Methods for improving callus formation and regeneration in plants | |
| WO2021019394A1 (en) | Modified plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220928 |
|
| EEER | Examination request |
Effective date: 20220928 |
|
| EEER | Examination request |
Effective date: 20220928 |