CA3034959A1 - Procedes d'amplification numerique du genome entier - Google Patents
Procedes d'amplification numerique du genome entier Download PDFInfo
- Publication number
- CA3034959A1 CA3034959A1 CA3034959A CA3034959A CA3034959A1 CA 3034959 A1 CA3034959 A1 CA 3034959A1 CA 3034959 A CA3034959 A CA 3034959A CA 3034959 A CA3034959 A CA 3034959A CA 3034959 A1 CA3034959 A1 CA 3034959A1
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- Prior art keywords
- genomic dna
- double stranded
- amplification
- dna
- aqueous
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention décrit un procédé d'amplification d'ADN génomique, tel que l'amplification du génome entier, comprenant l'utilisation d'un système transposase pour produire des fragments de l'ADN génomique comprenant des sites de liaison aux amorces, l'isolement dans l'huile de chaque fragment à l'intérieur de sa propre microgouttelette aqueuse conjointement aux réactifs d'amplification PCR, l'amplification de chaque fragment à l'intérieur de sa propre microgouttelette aqueuse, la désémulsification des microgouttelettes pour obtenir les amplicons et le séquençage des amplicons.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2016/097520 WO2018039969A1 (fr) | 2016-08-31 | 2016-08-31 | Procédés d'amplification numérique du génome entier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3034959A1 true CA3034959A1 (fr) | 2018-03-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CA3034959A Abandoned CA3034959A1 (fr) | 2016-08-31 | 2016-08-31 | Procedes d'amplification numerique du genome entier |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20210285038A1 (fr) |
| EP (1) | EP3507379A4 (fr) |
| JP (1) | JP6882453B2 (fr) |
| CN (1) | CN109923214A (fr) |
| AU (1) | AU2016421196A1 (fr) |
| CA (1) | CA3034959A1 (fr) |
| MX (1) | MX2019002376A (fr) |
| RU (1) | RU2736351C2 (fr) |
| WO (1) | WO2018039969A1 (fr) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3567116A1 (fr) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Procédés et systèmes de traitement de polynucléotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2014124338A1 (fr) | 2013-02-08 | 2014-08-14 | 10X Technologies, Inc. | Génération de codes à barres de polynucléotides |
| WO2015157567A1 (fr) | 2014-04-10 | 2015-10-15 | 10X Genomics, Inc. | Dispositifs fluidiques, systèmes et procédés permettant d'encapsuler et de séparer des réactifs, et leurs applications |
| MX2016016902A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas. |
| CN107427808B (zh) | 2015-01-12 | 2020-10-23 | 10X基因组学有限公司 | 用于制备核酸测序文库的方法和系统以及用其制备的文库 |
| US10697000B2 (en) | 2015-02-24 | 2020-06-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| CN117512066A (zh) | 2017-01-30 | 2024-02-06 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
| JP2020522243A (ja) | 2017-05-23 | 2020-07-30 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸のマルチプレックス末端タギング増幅 |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN109526228B (zh) | 2017-05-26 | 2022-11-25 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| WO2019084043A1 (fr) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | Méthodes et systèmes de préparation d'acide nucléique et d'analyse de chromatine |
| WO2019099751A1 (fr) | 2017-11-15 | 2019-05-23 | 10X Genomics, Inc. | Perles de gel fonctionnalisées |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| EP3752832A1 (fr) | 2018-02-12 | 2020-12-23 | 10X Genomics, Inc. | Procédés de caractérisation d'analytes multiples à partir de cellules individuelles ou de populations cellulaires |
| EP3775271A1 (fr) | 2018-04-06 | 2021-02-17 | 10X Genomics, Inc. | Systèmes et procédés de contrôle de qualité dans un traitement de cellules uniques |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| WO2020168013A1 (fr) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Procédés de traitement de molécules d'acides nucléiques |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2021222267A1 (fr) * | 2020-04-28 | 2021-11-04 | President And Fellows Of Harvard College | Systèmes et procédés de détermination de virus ou d'autres pathogènes |
| RU2752840C1 (ru) * | 2020-12-17 | 2021-08-09 | Общество с ограниченной ответственностью «ДНК-дисплей» | Способ штрихкодирования ампликонов при подготовке таргетных библиотек ДНК к массовому параллельному секвенированию |
| CN115807058B (zh) * | 2022-12-02 | 2023-06-30 | 中国科学院水生生物研究所 | 一种低偏向的单精子全基因组扩增方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1590477B1 (fr) * | 2003-01-29 | 2009-07-29 | 454 Corporation | PROCÉDÉ D'AMPLIFICATION ET DE SÉQUENçAGE D'ACIDES NUCLÉIQUES |
| DE602004029560D1 (de) * | 2003-03-07 | 2010-11-25 | Rubicon Genomics Inc | Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| WO2012061832A1 (fr) * | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Liaison entre des lectures de séquences à l'aide de codes marqueurs appariés |
| US9617598B2 (en) * | 2011-05-27 | 2017-04-11 | President And Fellows Of Harvard College | Methods of amplifying whole genome of a single cell |
| US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| CN107075509B (zh) * | 2014-05-23 | 2021-03-09 | 数字基因公司 | 通过数字化转座子的单倍体组测定 |
| US10017759B2 (en) * | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| CN105463066B (zh) * | 2014-09-04 | 2019-05-17 | 中国科学院北京基因组研究所 | 一种dna扩增方法 |
| MX2018000729A (es) * | 2015-07-17 | 2018-09-06 | Harvard College | Métodos para amplificar las secuencias de ácido nucleico. |
-
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- 2016-08-31 MX MX2019002376A patent/MX2019002376A/es unknown
- 2016-08-31 WO PCT/CN2016/097520 patent/WO2018039969A1/fr unknown
- 2016-08-31 CA CA3034959A patent/CA3034959A1/fr not_active Abandoned
- 2016-08-31 CN CN201680090529.4A patent/CN109923214A/zh active Pending
- 2016-08-31 RU RU2019108270A patent/RU2736351C2/ru active
- 2016-08-31 AU AU2016421196A patent/AU2016421196A1/en not_active Abandoned
- 2016-08-31 EP EP16914535.6A patent/EP3507379A4/fr not_active Withdrawn
- 2016-08-31 US US16/326,405 patent/US20210285038A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20210285038A1 (en) | 2021-09-16 |
| RU2019108270A3 (fr) | 2020-10-01 |
| WO2018039969A1 (fr) | 2018-03-08 |
| EP3507379A1 (fr) | 2019-07-10 |
| CN109923214A (zh) | 2019-06-21 |
| RU2019108270A (ru) | 2020-10-01 |
| JP6882453B2 (ja) | 2021-06-02 |
| EP3507379A4 (fr) | 2020-05-13 |
| RU2736351C2 (ru) | 2020-11-16 |
| AU2016421196A1 (en) | 2019-03-21 |
| JP2019531714A (ja) | 2019-11-07 |
| MX2019002376A (es) | 2019-06-20 |
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