CA3026604A1 - Isolat de proteines de colza natives de qualite alimentaire et procede pour l'obtenir - Google Patents
Isolat de proteines de colza natives de qualite alimentaire et procede pour l'obtenirInfo
- Publication number
- CA3026604A1 CA3026604A1 CA3026604A CA3026604A CA3026604A1 CA 3026604 A1 CA3026604 A1 CA 3026604A1 CA 3026604 A CA3026604 A CA 3026604A CA 3026604 A CA3026604 A CA 3026604A CA 3026604 A1 CA3026604 A1 CA 3026604A1
- Authority
- CA
- Canada
- Prior art keywords
- protein isolate
- rapeseed protein
- aqueous liquid
- native rapeseed
- native
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 111
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 111
- 235000004977 Brassica sinapistrum Nutrition 0.000 title claims abstract description 68
- 238000000034 method Methods 0.000 title claims abstract description 28
- 235000013305 food Nutrition 0.000 title claims abstract description 27
- 244000188595 Brassica sinapistrum Species 0.000 title claims abstract 20
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 235000012054 meals Nutrition 0.000 claims abstract description 14
- 235000019484 Rapeseed oil Nutrition 0.000 claims abstract description 13
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 239000002244 precipitate Substances 0.000 claims abstract description 10
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 230000007935 neutral effect Effects 0.000 claims abstract description 6
- 239000002253 acid Substances 0.000 claims abstract description 5
- 238000005406 washing Methods 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 15
- 238000005119 centrifugation Methods 0.000 claims description 9
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims description 7
- 235000002949 phytic acid Nutrition 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 238000013060 ultrafiltration and diafiltration Methods 0.000 claims description 6
- 239000012266 salt solution Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 235000018102 proteins Nutrition 0.000 description 91
- 240000002791 Brassica napus Species 0.000 description 47
- 235000019198 oils Nutrition 0.000 description 15
- 239000012141 concentrate Substances 0.000 description 11
- 239000000284 extract Substances 0.000 description 11
- 230000000813 microbial effect Effects 0.000 description 10
- 101710190853 Cruciferin Proteins 0.000 description 9
- 238000000605 extraction Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 101710202365 Napin Proteins 0.000 description 7
- 150000003839 salts Chemical class 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 235000019779 Rapeseed Meal Nutrition 0.000 description 5
- 239000006185 dispersion Substances 0.000 description 5
- 235000013372 meat Nutrition 0.000 description 5
- 239000004465 oilseed meal Substances 0.000 description 5
- 239000004456 rapeseed meal Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000251468 Actinopterygii Species 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000000055 blue native polyacrylamide gel electrophoresis Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 125000004383 glucosinolate group Chemical group 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 230000031787 nutrient reservoir activity Effects 0.000 description 3
- 238000009928 pasteurization Methods 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- 240000000385 Brassica napus var. napus Species 0.000 description 2
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 2
- DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 description 2
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- URXZXNYJPAJJOQ-UHFFFAOYSA-N Erucic acid Natural products CCCCCCC=CCCCCCCCCCCCC(O)=O URXZXNYJPAJJOQ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 238000007696 Kjeldahl method Methods 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000001627 detrimental effect Effects 0.000 description 2
- 238000011026 diafiltration Methods 0.000 description 2
- DPUOLQHDNGRHBS-KTKRTIGZSA-N erucic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-KTKRTIGZSA-N 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000002198 insoluble material Substances 0.000 description 2
- 239000000693 micelle Substances 0.000 description 2
- 238000001471 micro-filtration Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000001542 size-exclusion chromatography Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 244000178993 Brassica juncea Species 0.000 description 1
- 235000011332 Brassica juncea Nutrition 0.000 description 1
- 235000014700 Brassica juncea var napiformis Nutrition 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 238000011993 High Performance Size Exclusion Chromatography Methods 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 108010084695 Pea Proteins Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 240000000359 Triticum dicoccon Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000002269 clear native polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 201000007235 gluten allergy Diseases 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- WPEXVRDUEAJUGY-UHFFFAOYSA-B hexacalcium;(2,3,4,5,6-pentaphosphonatooxycyclohexyl) phosphate Chemical compound [Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])(=O)OC1C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C1OP([O-])([O-])=O WPEXVRDUEAJUGY-UHFFFAOYSA-B 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 235000021125 infant nutrition Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 238000001426 native polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000019702 pea protein Nutrition 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 229940001941 soy protein Drugs 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Peptides Or Proteins (AREA)
- Fodder In General (AREA)
- Edible Oils And Fats (AREA)
Abstract
La présente invention concerne un isolat de protéines de colza natives comprenant de 40 à 65 % pds de cruciférines et de 35 à 60 % pds de napines et présentant une solubilité d'au moins 88 % sur une plage de pH de 3 à 10 à une température de 23 ± 2 °C avec une teneur en microbes inférieure à 1000 UFC/g. Elle concerne également l'utilisation de l'isolat de protéines de colza natives dans des produits alimentaires ou des produits alimentaires pour animaux.L'invention concerne également un procédé d'obtention d'un isolat de protéines de colza natives comprenant les étapes suivantes : i) mélange de farine d'huile de colza pressée à froid avec un liquide aqueux à une température de 45 à 65 °C ; ii) séparation du liquide aqueux du mélange obtenu à l'étape i) ; iii) écrémage du liquide aqueux obtenu à l'étape ii) ; iv) ajustement du pH du liquide aqueux écrémé obtenu à l'étape iii) à un pH neutre par addition d'acide ou de base, et mélange avec un agent de précipitation pour obtenir un précipité ; v) élimination du précipité obtenu à l'étape iv) pour obtenir un liquide aqueux ; vi) concentration et lavage du liquide aqueux obtenu à l'étape v) ; vii) isolement de l'isolat de protéines de colza natives à partir du liquide aqueux concentré et lavé obtenu à l'étape vi) au moyen d'un séchage.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16178347.7 | 2016-07-07 | ||
| EP16178347 | 2016-07-07 | ||
| EP17166994.8 | 2017-04-19 | ||
| EP17166994 | 2017-04-19 | ||
| PCT/EP2017/066870 WO2018007491A1 (fr) | 2016-07-07 | 2017-07-06 | Isolat de protéines de colza natives de qualité alimentaire et procédé pour l'obtenir |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3026604A1 true CA3026604A1 (fr) | 2018-01-11 |
Family
ID=59270046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3026604A Pending CA3026604A1 (fr) | 2016-07-07 | 2017-07-06 | Isolat de proteines de colza natives de qualite alimentaire et procede pour l'obtenir |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP3481215A1 (fr) |
| CA (1) | CA3026604A1 (fr) |
| WO (1) | WO2018007491A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017102535A1 (fr) | 2015-12-17 | 2017-06-22 | Dsm Ip Assets B.V. | Isolat de protéines de colza, aliment comprenant l'isolat et son utilisation comme agent émulsifiant ou moussant |
| PL3481217T3 (pl) | 2016-07-07 | 2020-11-16 | Dsm Ip Assets B.V. | Izolat białka rzepakowego, żywność zawierająca izolat i jego zastosowanie jako środka pianotwórczego lub emulgującego |
| EP3481216B2 (fr) | 2016-07-07 | 2024-03-06 | DSM IP Assets B.V. | Procédé de fabrication d'un isolat de protéine de colza ainsi qu'un isolat de protéines obtenu selon le procédé |
| CN109788777B (zh) | 2016-07-07 | 2023-04-04 | 帝斯曼知识产权资产管理有限公司 | 包含油菜籽蛋白质分离物的乳剂 |
| US20220304332A1 (en) * | 2019-06-21 | 2022-09-29 | Dsm Ip Assets B.V. | Heat stable rapeseed protein composition |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030021884A1 (en) | 2001-05-04 | 2003-01-30 | Murray E. Donald | Canola protein isolate functionality I |
| US20070015910A1 (en) * | 2001-11-20 | 2007-01-18 | Barker Larry D | Continuous process for production of oil seed protein isolate |
| NZ549134A (en) | 2004-01-20 | 2009-12-24 | Burcon Nutrascience Mb Corp | Canola protein isolate with increased 2S protein and decreased 7S protein concentrations, method contains two selective membrane filtrations and a heat treatment step. |
| US20080181990A1 (en) | 2007-01-26 | 2008-07-31 | Ledbetter Kati R | Compositions comprising wheat protein isolate and related methods |
| US8623445B2 (en) | 2008-05-16 | 2014-01-07 | Bio-Extraction Inc. | Protein concentrates and isolates, and processes for the production thereof |
| US20100041871A1 (en) | 2008-08-18 | 2010-02-18 | Segall Kevin I | Preparation of Canola Protein Isolate from Canola Oil Seeds ("Blendertein") |
| UA113181C2 (xx) * | 2011-07-28 | 2016-12-26 | Виділення білка з олієвмісного насіння | |
| BR112015001577A2 (pt) | 2012-07-26 | 2017-07-04 | Solae Llc | agente de emulsificação, produto alimentício, método para a fabricação de um produto alimentício e agente de emulsificação e de estabilização. |
| EA201500950A1 (ru) * | 2013-03-18 | 2016-02-29 | ДСМ АйПи АССЕТС Б.В. | Способ экстракции белка из масличных семян |
| EP3099184A2 (fr) | 2014-01-29 | 2016-12-07 | Nestec S.A. | Produits alimentaires à la viande pour animaux de compagnie sans gluten de blé |
| DE102014005466A1 (de) | 2014-04-12 | 2015-10-15 | Klaus Düring | Verfahren zur Gewinnung von Napin und Cruciferin oder einem Gemisch davon aus Raps |
-
2017
- 2017-07-06 WO PCT/EP2017/066870 patent/WO2018007491A1/fr unknown
- 2017-07-06 CA CA3026604A patent/CA3026604A1/fr active Pending
- 2017-07-06 EP EP17734755.6A patent/EP3481215A1/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP3481215A1 (fr) | 2019-05-15 |
| WO2018007491A1 (fr) | 2018-01-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
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| EEER | Examination request |
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| EEER | Examination request |
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| EEER | Examination request |
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