CA2990237A1 - Therapeutic uses of berberine formulations - Google Patents
Therapeutic uses of berberine formulations Download PDFInfo
- Publication number
- CA2990237A1 CA2990237A1 CA2990237A CA2990237A CA2990237A1 CA 2990237 A1 CA2990237 A1 CA 2990237A1 CA 2990237 A CA2990237 A CA 2990237A CA 2990237 A CA2990237 A CA 2990237A CA 2990237 A1 CA2990237 A1 CA 2990237A1
- Authority
- CA
- Canada
- Prior art keywords
- inhibitors
- berberine
- biologically equivalent
- group
- equivalent analogue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 229940091258 selenium supplement Drugs 0.000 description 1
- CYOHGALHFOKKQC-UHFFFAOYSA-N selumetinib Chemical compound OCCONC(=O)C=1C=C2N(C)C=NC2=C(F)C=1NC1=CC=C(Br)C=C1Cl CYOHGALHFOKKQC-UHFFFAOYSA-N 0.000 description 1
- 229950010746 selumetinib Drugs 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 238000009097 single-agent therapy Methods 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 231100000046 skin rash Toxicity 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 229940037001 sodium edetate Drugs 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000012421 spiking Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- TUNFSRHWOTWDNC-HKGQFRNVSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCC[14C](O)=O TUNFSRHWOTWDNC-HKGQFRNVSA-N 0.000 description 1
- 238000011200 topical administration Methods 0.000 description 1
- 229940042129 topical gel Drugs 0.000 description 1
- 235000010487 tragacanth Nutrition 0.000 description 1
- 239000000196 tragacanth Substances 0.000 description 1
- 229940116362 tragacanth Drugs 0.000 description 1
- 229960004066 trametinib Drugs 0.000 description 1
- LIRYPHYGHXZJBZ-UHFFFAOYSA-N trametinib Chemical compound CC(=O)NC1=CC=CC(N2C(N(C3CC3)C(=O)C3=C(NC=4C(=CC(I)=CC=4)F)N(C)C(=O)C(C)=C32)=O)=C1 LIRYPHYGHXZJBZ-UHFFFAOYSA-N 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
- 229960005066 trisodium edetate Drugs 0.000 description 1
- JFJZZMVDLULRGK-URLMMPGGSA-O tubocurarine Chemical compound C([C@H]1[N+](C)(C)CCC=2C=C(C(=C(OC3=CC=C(C=C3)C[C@H]3C=4C=C(C(=CC=4CCN3C)OC)O3)C=21)O)OC)C1=CC=C(O)C3=C1 JFJZZMVDLULRGK-URLMMPGGSA-O 0.000 description 1
- 239000006217 urethral suppository Substances 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 239000003871 white petrolatum Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- XOOUIPVCVHRTMJ-UHFFFAOYSA-L zinc stearate Chemical compound [Zn+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O XOOUIPVCVHRTMJ-UHFFFAOYSA-L 0.000 description 1
Classifications
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Abstract
Uses of pharmaceutical compositions comprising berberine for treatment of dermatologic toxicities and other skin disorders.
Description
THERAPEUTIC USES OF BERBERINE FORMULATIONS
BACKGROUND OF THE INVENTION
[001] Red face related skin disorders, which share symptomatic similarities and probably pathological causes, include rosacea, acne vulgaris, seborrheic dermatitis, photodermatitis and contact dermatitis. These red face related conditions may range from feelings of heat and sensitivity to flushing or burning with intense sensitivity. Patients with red face related skin disorders often exhibit extreme sensitivity to environmental and topical factors. Steroid-induced rosacea-like dermatitis (or steroid rosacea) is papular or pustular lesions with erythematous and edematous base with or without telangiectasia, which is caused by prolonged application of topical steroids to the face or as a rebound condition after discontinuation of topical steroids.
BACKGROUND OF THE INVENTION
[001] Red face related skin disorders, which share symptomatic similarities and probably pathological causes, include rosacea, acne vulgaris, seborrheic dermatitis, photodermatitis and contact dermatitis. These red face related conditions may range from feelings of heat and sensitivity to flushing or burning with intense sensitivity. Patients with red face related skin disorders often exhibit extreme sensitivity to environmental and topical factors. Steroid-induced rosacea-like dermatitis (or steroid rosacea) is papular or pustular lesions with erythematous and edematous base with or without telangiectasia, which is caused by prolonged application of topical steroids to the face or as a rebound condition after discontinuation of topical steroids.
[002] Dermatologic toxicities are known cutaneous adverse events associated with targeted therapies or immunotherapy and share similar symptoms and probable pathologic causes of the red face-related skin disorders. Targeted therapies such as epidermal growth factor receptor (EGFR) inhibitors, multityrosine kinase (MTK) inhibitors, MEK inhibitors, phosphoinositide
3-kinase (PI3K) inhibitors, protein kinase B (AKT) inhibitors, BRAF
inhibitors, HER2 inhibitor, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC inhibitors, proteasome inhibitors, and retinoid X receptor (RXR) agonists; immunotherapies such as cancer vaccines, cytokine agents (e.g., granulocyte-macrophage colony-stimulating factor (GM-CSF), interferons, and interleukin-2 (IL-2)), cell therapies (e.g., tumor-infiltrating lymphocytes (TILs), T-cell receptor (TCR)-engineered peripheral blood lymphocytes (PBL), and chimeric antigen receptor (CAR)-engineered PBL), immune checkpoint protein inhibitors (e.g., PD-1, PD-L1, CTLA-4, TIM-3, LAG-3, BTLA, VISTA, and TIG1T), and immune checkpoint protein stimulators (e.g., 0028, ICOS, 4-1BB, 0X40, BITR, CD27, TWEAKR, HVEM, TIM-1, and CD-40); or a combination of any of the above therapies could induce toxicities including papulopustular rash, maculopapular rash, erythema, telangiectasias flushing, paronychia and fissure, hair changes, xerosis, mucositis, pruritus, and hand-foot skin reaction, which may occur in more than 90% of patients and may also superinfected with bacteria, such as staphylococcus aureus (Wollenberg, Kroth et al., Cutaneous side effects of EGFR inhibitors--appearance and management, Dtsch Med Wochenschr 2010; Lacouture, Maitland et al., A proposed EGFR inhibitor dermatologic adverse event-specific grading scale from the MASCO skin toxicity study group, Support Care Cancer, 2010; Curry, Torres-Cabala et al., Dermatologic toxicities to targeted cancer therapy:
shared clinical and histologic adverse skin reactions, International Journal of Dermatology, 2014;
Jeffrey S. Weber et. al., Toxicities of Immunotherapy for the Practitioner, Journal of Clinical Oncology, Vol. 33, 2015; Grace K. Dy and Alex A. Adjei, Understanding, Recognizing, and Managing Toxicities of Targeted Anticancer Therapies, CA Cancer J Olin, Vol.
63, 2013; Ahmad Tarhini, Immune-Mediated Adverse Events Associated with lpilimumab CTLA-4 Blockade Therapy: The Underlying Mechanisms and Clinical Management, Scientifica, 2013;
J Larkin et al., Combined Nivolumab and lpilimumab or Monotherapy in Untreated Melanoma, N.
Engl, J. Med., Vol. 373, 2015). Histopathologic findings of such skin toxicities showed that inflammation is frequently involved and leads to acneiform skin rash. A papulopustular rash was more frequently reported on EGFR inhibitors like cetuximab (83% of patients) and afatinib (90%
of patients), and MEK inhibitors like selumetinib (93% of patients) and trametinib (80% of patients) therapy. A
maculopapular rash was more commonly described with PI3K inhibitors like 8KM-120 (37% of patients), MK2206 (52% of patients) therapy, immune checkpoint protein inhibitors like anti-CTLA-4 inhibitor ipilimumab (33% of patients), anti-PD-1 inhibitor nivolumab (26% of patients), or combination of ipilimumab and nivolimumab (more than 40% of patients).
[003] Berberine (Natural Yellow 18, 5,6-dihydro-9,10-dimethoxybenzo(g)-1,3-benzodioxolo (5,6-a) quinolizinium) is an isoquinoline alkaloid present in herb plants, such as coptis (Coptidis rhizome), phellodenron, Scutellaria baicalensis, Mahonia aquifolium and berberis. Berberine and its derivatives have been found to have antimicrobial and antimalarial activities. It can act against various kinds of pathogens such as fungi, saccharomycete, parasite, bacterium and virus.
inhibitors, HER2 inhibitor, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC inhibitors, proteasome inhibitors, and retinoid X receptor (RXR) agonists; immunotherapies such as cancer vaccines, cytokine agents (e.g., granulocyte-macrophage colony-stimulating factor (GM-CSF), interferons, and interleukin-2 (IL-2)), cell therapies (e.g., tumor-infiltrating lymphocytes (TILs), T-cell receptor (TCR)-engineered peripheral blood lymphocytes (PBL), and chimeric antigen receptor (CAR)-engineered PBL), immune checkpoint protein inhibitors (e.g., PD-1, PD-L1, CTLA-4, TIM-3, LAG-3, BTLA, VISTA, and TIG1T), and immune checkpoint protein stimulators (e.g., 0028, ICOS, 4-1BB, 0X40, BITR, CD27, TWEAKR, HVEM, TIM-1, and CD-40); or a combination of any of the above therapies could induce toxicities including papulopustular rash, maculopapular rash, erythema, telangiectasias flushing, paronychia and fissure, hair changes, xerosis, mucositis, pruritus, and hand-foot skin reaction, which may occur in more than 90% of patients and may also superinfected with bacteria, such as staphylococcus aureus (Wollenberg, Kroth et al., Cutaneous side effects of EGFR inhibitors--appearance and management, Dtsch Med Wochenschr 2010; Lacouture, Maitland et al., A proposed EGFR inhibitor dermatologic adverse event-specific grading scale from the MASCO skin toxicity study group, Support Care Cancer, 2010; Curry, Torres-Cabala et al., Dermatologic toxicities to targeted cancer therapy:
shared clinical and histologic adverse skin reactions, International Journal of Dermatology, 2014;
Jeffrey S. Weber et. al., Toxicities of Immunotherapy for the Practitioner, Journal of Clinical Oncology, Vol. 33, 2015; Grace K. Dy and Alex A. Adjei, Understanding, Recognizing, and Managing Toxicities of Targeted Anticancer Therapies, CA Cancer J Olin, Vol.
63, 2013; Ahmad Tarhini, Immune-Mediated Adverse Events Associated with lpilimumab CTLA-4 Blockade Therapy: The Underlying Mechanisms and Clinical Management, Scientifica, 2013;
J Larkin et al., Combined Nivolumab and lpilimumab or Monotherapy in Untreated Melanoma, N.
Engl, J. Med., Vol. 373, 2015). Histopathologic findings of such skin toxicities showed that inflammation is frequently involved and leads to acneiform skin rash. A papulopustular rash was more frequently reported on EGFR inhibitors like cetuximab (83% of patients) and afatinib (90%
of patients), and MEK inhibitors like selumetinib (93% of patients) and trametinib (80% of patients) therapy. A
maculopapular rash was more commonly described with PI3K inhibitors like 8KM-120 (37% of patients), MK2206 (52% of patients) therapy, immune checkpoint protein inhibitors like anti-CTLA-4 inhibitor ipilimumab (33% of patients), anti-PD-1 inhibitor nivolumab (26% of patients), or combination of ipilimumab and nivolimumab (more than 40% of patients).
[003] Berberine (Natural Yellow 18, 5,6-dihydro-9,10-dimethoxybenzo(g)-1,3-benzodioxolo (5,6-a) quinolizinium) is an isoquinoline alkaloid present in herb plants, such as coptis (Coptidis rhizome), phellodenron, Scutellaria baicalensis, Mahonia aquifolium and berberis. Berberine and its derivatives have been found to have antimicrobial and antimalarial activities. It can act against various kinds of pathogens such as fungi, saccharomycete, parasite, bacterium and virus.
[004] Berberine also has anti-inflammatory function, yet the exact mechanism is unknown.
[005] U.S. Pat. No. 6,440,465 pertains to topical skin formulations of glucosamine in an emollient base which contains berberine for the treatment of psoriasis. U.S.
Patent Publication No. 2005/0158404 pertains to a nutritional product, dietary supplement or pharmaceutical composition which contains vitamin A, vitamin E, selenium. vitamin B6, zinc, chromium, and an herbal source of berberine for the treatment of acne in oral administration.
U.S. Pat. No.
Patent Publication No. 2005/0158404 pertains to a nutritional product, dietary supplement or pharmaceutical composition which contains vitamin A, vitamin E, selenium. vitamin B6, zinc, chromium, and an herbal source of berberine for the treatment of acne in oral administration.
U.S. Pat. No.
6,974,799 relates to topical compositions comprising a tripeptide (N-palmitoyl-Gly-His-Lys) and a tetrapeptide (N-palmitoyl-Gly-Gln-Pro-Arg) for the treatment of visible signs of aging including wrinkles, stretch marks, dark circles. The formulation may contain additional ingredients, including berberine. In these inventions, berberine is included as one of the many ingredients and its concentration is not specified.
[006] U.S. Patent Publication 2004/0146539 relates to topical nutraceutical compositions with body slimming and tone-firming anti-aging benefits that may be used to treat skin aging, skin wrinkle, skin exfoliating, acne, rosacea and other skin problems. The composition of this invention includes antimicrobial agents selected from several agents including berberine. In these nutraceutical compositions, berberine is included as one of the many ingredients and its concentration is not specified. There has been a 10% Mahonia aquifolium cream (RelievaTM, Apollo Pharmaceutical Canada Inc) containing 0.1% berberine for the treatment of psoriasis.
[006] U.S. Patent Publication 2004/0146539 relates to topical nutraceutical compositions with body slimming and tone-firming anti-aging benefits that may be used to treat skin aging, skin wrinkle, skin exfoliating, acne, rosacea and other skin problems. The composition of this invention includes antimicrobial agents selected from several agents including berberine. In these nutraceutical compositions, berberine is included as one of the many ingredients and its concentration is not specified. There has been a 10% Mahonia aquifolium cream (RelievaTM, Apollo Pharmaceutical Canada Inc) containing 0.1% berberine for the treatment of psoriasis.
[007] U.S. Patent Publication 2012/0165357 discloses the use of berberine to treat various red face related skin disorders but it does not disclose any specific formulations of berberine that would be found to be effective for the treatment of specific conditions,
[008] Therefore, there is still a need to develop new effective methods for the treatment of various red face related skin disorders as well as dermatologic toxicities induced by targeted therapy and/or immunotherapy.
SUMMARY OF THE INVENTION
SUMMARY OF THE INVENTION
[009] The present invention provides pharmaceutical compositions for the treatment and/or prevention of red face related skin disorders and dermatologic toxicities induced by targeted therapy and/or immunotherapy. The provided formulations are either cream-based (i.e., cream) formulations or gel-based formulations.
[010] In particular, the invention provides a pharmaceutical composition comprising berberine, wherein said composition is a cream formulation comprising a water phase and an oil phase.
[011] In one embodiment, the concentration of berberine in the provided cream formulations is between 0.01% and 10%, preferably between 0.01% and 0.3% w/w, more preferably between 0.1% and 0.2% w/w, even more preferably between 0.1% and 0.15% w/w, and most preferably about 0.12% w/w.
[012] Unless explicitly stated otherwise, whenever the application describes amounts or concentrations in the w/w format, the weight of each ingredient is by the total weight of the formulation.
[013] The pharmaceutical compositions of the invention may further comprise a penetration enhancer.
[014] In one embodiment, the penetration enhancer is an anionic penetration enhancer.
[015] In another embodiment, the penetration enhancer comprises Tween 60 and glycerin.
[016] In one embodiment, berberine is the only pharmaceutically active component in the provided formulations.
[017] In one embodiment, the pharmaceutical compositions of the invention have a pH of between about 4 and about 7, and more preferably of about 5.5.
[018] In a preferred embodiment, the invention provides a pharmaceutical composition comprising berberine as the only pharmaceutically active component, wherein said berberine is at a concentration of between 0.1% and 0.2% w/w, wherein said composition is a cream formulation comprising a water phase and an oil phase, wherein said composition comprises a penetration enhancer, a preservative, and a stabilizer, and wherein said composition has a pH of between about 4 and about 7.
[019] In an even more preferred embodiment, the invention provides a pharmaceutical composition comprising berberine as the only pharmaceutically active component, wherein said berberine is at a concentration of about 0.12% w/w, wherein said composition is a cream formulation comprising a water phase and an oil phase, wherein said composition comprises a penetration enhancer, a preservative, and a stabilizer, and wherein said composition has a pH of about 5.5.
[020] In another embodiment, the invention provides a pharmaceutical composition comprising berberine, wherein said composition is a gel-based formulation, wherein said composition comprises an anionic penetration enhancer.
[021] In a preferred embodiment, the anionic penetration enhancer comprises sodium dodecyl sulfate (SDS).
[022] In one embodiment, in the gel-based pharmaceutical compositions provided by the invention, about 90% of an average particle size of the berberine is less than 10 pm.
[023] In another embodiment, in the gel-based pharmaceutical compositions provided by the invention, about 50% of an average particle size of the berberine is less than 4 pm.
[024] In one embodiment, the concentration of berberine in the provided gel-based formulations is between 0.01% and 0.3% w/w, more preferably between 0.1% and 0.2% w/w, even more preferably between 0.1% and 0.15% w/w, and most preferably about 0.12% w/w.
[025] The invention also provides methods of treating a red face related skin disorder comprising administering to a patient in need thereof a pharmaceutically effective amount of the pharmaceutical composition of the invention.
[026] In one embodiment, red face related skin disorder is selected from the group consisting of rosacea. acne vulgaris, seborrheic dermatitis, photodermatitis, contact dermatitis, steroid-induced rosacea-like dermatitis, and epidermal growth factor receptor (EGFR) inhibitor-induced skin disorder.
[027] The invention further provides methods of treating and/or preventing dermatologic toxicities induced by targeted therapy and/or immunotherapy comprising administering to a patient in need thereof a pharmaceutically effective amount of berberine and/or a biologically equivalent analogue thereof.
[028] In a preferred embodiment, the targeted therapy comprises therapies by EGFR
inhibitors; MTK inhibitors, MEK inhibitors, PI3K inhibitors, AKT inhibitors, BRAF inhibitors, HER2 inhibitor, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC inhibitors, proteasome inhibitors, and RXR agonists.
inhibitors; MTK inhibitors, MEK inhibitors, PI3K inhibitors, AKT inhibitors, BRAF inhibitors, HER2 inhibitor, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC inhibitors, proteasome inhibitors, and RXR agonists.
[029] In a preferred embodiment, the immunotherapy comprises therapies by cancer vaccines, cytokine agents (e.g., granulocyte-macrophage colony-stimulating factor (GM-CSF), interferons, and interleukin-2 (1L-2)), cell therapies (e.g., tumor-infiltrating lymphocytes (TILs), T-cell receptor (TCR)-engineered peripheral blood lymphocytes (PBL), and chimeric antigen receptor (CAR)-engineered PBL), immune checkpoint protein inhibitors (e.g., PD-1, PD-L1, CTLA-4, TIM-3, LAG-3, BTLA, VISTA, and TIGIT), and immuno checkpoint protein stimulators (e.g., CD28, 1COS, 4-1BB, 0X40, BITR, CD27, TWEAKR, HVEM, TIM-1, and CD-40).
[030] In a preferred embodiment, berberine and/or the biologically equivalent analogue thereof is administered to the patient in form of a topical pharmaceutical composition.
BRIEF DESCRIPTION OF THE DRAWINGS
BRIEF DESCRIPTION OF THE DRAWINGS
[031] The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
[032] Figure 1 is a plot of cumulative berberine penetrated (ng/cm2) vs time for six tested formulations of berberine.
[033] Figure 2 is a plot of cumulative berberine penetrated (ng/cm2) vs time for three gel suspension formulations of berberine (G22, G23 and G24).
[034] Figure 3 is a picture showing hematoxylin and eosin (H&E) staining of bilateral skin biopsies from nasolabial folds of a patient receiving afatinib and topically administered with a gel formulation (G23) on one side of his face.
[035] Figure 4 is a picture showing H&E staining of bilateral skin biopsies from nasolabial folds of the patient receiving the EGFR inhibitor afatinib and topically administered with a vehicle gel (G23 without berberine) on the other side of his face.
[036] Figure 5 is a plot of papule counts vs time for a patient receiving afatinib and topically administered with a gel formulation (G23) on one side of his face and a vehicle gel (G23 without berberine) on the other side of his face (* denotes P< 0.05 by Wilcoxon Signed Rank test).
[037] Figure 6 is a plot of pustule counts vs time for the patient receiving afatinib and topically administered with the gel formulation (G23) on one side of his face and the vehicle gel (G23 without berberine) on the other side of his face (* denotes P< 0.05 by Wilcoxon Signed Rank test).
[038] Figure 7 is a plot of total counts (papule plus pustule) vs time for the patient receiving afatinib and topically administered with the gel formulation (G23) on one side of his face and the vehicle gel (G23 without berberine) on the other side of his face (* denotes P< 0.05 by Wilcoxon Signed Rank test).
DETAILED DESCRIPTION OF THE INVENTION
DETAILED DESCRIPTION OF THE INVENTION
[039] Depending on a particular disorder being treated, it is important that berberine can efficiently penetrate the skin. Berberine is a hydrophilic compound (a partition coefficient of 1.07 in an octanol-water system) which makes it hard for berberine to penetrate through the stratum corneum (SC) to reach the target site, e.g. dermis or epidermis, where red face related skin disorders or targeted therapy-induced dermatologic toxicities may occur.
Further, berberine is rather soluble (solubility of 1.57 mg/ml) and will therefore be quickly released into the target cells, leading to a temporary effect.
Further, berberine is rather soluble (solubility of 1.57 mg/ml) and will therefore be quickly released into the target cells, leading to a temporary effect.
[040] The present invention thus provides pharmaceutical compositions having an improved penetration rate of berberine for the treatment and/or prevention of red face related skin disorders and dermatological toxicities induced by targeted therapy and/or immunotherapy. The provided formulations are either cream-based (i.e., cream) formulations or gel-based formulations.
[041] In particular, the invention provides a pharmaceutical composition comprising berberine, wherein said composition is a cream formulation comprising a water phase and an oil phase.
[042] Because the cream formulations of the invention may promote the penetration of berberine into the skin, a relatively small amount of berberine is sufficient to achieve desired treating effects. In one embodiment, the concentration of berberine in the provided cream formulations is between 0.01% and 10% w/w, preferably 0.01% and 0.3% w/w, more preferably between 0.1% and 0.2% w/w, even more preferably between 0.1% and 0.15% w/w, and most preferably about 0.12% w/w, on the basis of the total weight of the formulation.
[043] The pharmaceutical compositions of the invention may further comprise a penetration enhancer.
[044] In one embodiment, the penetration enhancer is an anionic penetration enhancer.
For example, the anionic penetration enhancer may comprise sodium dodecyl sulfate (SDS).
For example, the anionic penetration enhancer may comprise sodium dodecyl sulfate (SDS).
[045] In another embodiment, the penetration enhancer comprises Tween0 60 and glycerin.
The cream formulations of the invention preferably include Tween 60 and glycerin as penetration enhancers. When the same penetration enhancers are used in non-cream formulations, they do not result in an improved penetration rate, suggesting that there is something unique about the cream-based formulations.
The cream formulations of the invention preferably include Tween 60 and glycerin as penetration enhancers. When the same penetration enhancers are used in non-cream formulations, they do not result in an improved penetration rate, suggesting that there is something unique about the cream-based formulations.
[046] In one embodiment, berberine is the only pharmaceutically active component in the provided formulations. Even if an ingredient of the provided formulations may be an active component in prior art formulations for purposes other than treatment of dermatologic toxicities induced by targeted therapy or immunotherapy, it is still considered a pharmaceutical excipient for the purposes of the provided formulations as long as this ingredient is not present at an amount sufficient to effectively treat dermatologic toxicities induced by targeted therapy or immunotherapy.
[047] In one embodiment, the pharmaceutical compositions of the invention have a pH of between about 4 and about 7, and more preferably of about 5.5.
[048] In a preferred embodiment, the invention provides a pharmaceutical composition comprising berberine as the only pharmaceutically active component, wherein said berberine is at a concentration of between 0.1% and 0.2% w/w, wherein said composition is a cream formulation comprising a water phase and an oil phase, wherein said composition comprises a penetration enhancer, a preservative, and a stabilizer, and wherein said composition has a pH of between about 4 and about 7.
[049] In an even more preferred embodiment, the invention provides a pharmaceutical composition comprising berberine as the only pharmaceutically active component, wherein said berberine is at a concentration of about 0.12% w/w, wherein said composition is a cream formulation comprising a water phase and an oil phase, wherein said composition comprises a penetration enhancer, a preservative, and a stabilizer, and wherein said composition has a pH of about 5.5.
[050] It was very surprisingly and unexpectedly found that the cream formulations of the invention have a superior penetration rate compared to non-cream berberine formulations.
[051] In another embodiment, the invention provides a pharmaceutical composition comprising berberine, wherein said composition is a gel-based formulation, wherein said composition comprises an anionic penetration enhancer.
[052] In a preferred embodiment, the anionic penetration enhancer comprises sodium dodecyl sulfate (SDS). Including SDS as an anionic penetration enhancer results in the provided gel-based formulations being hydrophobic (a partition coefficient of 50.1 in an octanol-water system) and having a dramatically lower solubility of about 0.011 mg/ml, allowing for a slow release of berberine into the target cells, resulting in an extended release profile.
[053] It was found in the present invention that, in a pH of between 4 and 7, berberine solubility in the presence of SDS ranges from 0.01 to 0.06 mg/mL, i.e., 25 to 150 times lower than aqueous berberine solubility (1.57 mg/mL), and is relatively low at pH
5.5.
5.5.
[054] It was surprisingly found that out of all tested penetration enhancers (SDS, glycerol, propylene glycol, PEG 400, ethanol, and Tween ), the addition of SDS in the gel-based formulations resulted in the most enhanced penetration rate and increased local concentration of berberine in epidermis and dermis.
[055] In one embodiment, in the gel-based pharmaceutical compositions provided by the invention, about 90% of an average particle size of the berberine is less than 10 pm.
[056] In another embodiment, in the gel-based pharmaceutical compositions provided by the invention, about 50% of an average particle size of the berberine is less than 4 pm.
[057] It was also surprisingly found that in the gel-based formulations there was a positive correlation between the amount of SDS and the penetration rate, and negative correlation between the size of berberine and the penetration rate.
[058] Because the gel-based formulations of the invention may promote the penetration of berberine into the skin, a relatively small amount of berberine is sufficient to achieve desired treating effects. In one embodiment, the concentration of berberine in the provided gel-based formulations is between 0.01% and 0.3% w/w, more preferably between 0.1% and 0.2% w/w, even more preferably between 0.1% and 0.15% w/w, and most preferably about 0.12% w/w, on the basis of the total weight of the formulation.
[059] The invention also provides methods of treating a red face related skin disorder comprising administering to a patient in need thereof a pharmaceutically effective amount of the pharmaceutical composition of the invention.
[060] In one embodiment, red face related skin disorder is selected from the group consisting of rosacea, acne vulgaris, seborrheic dermatitis, photodermatitis, contact dermatitis, steroid-induced rosacea-like dermatitis, and epidermal growth factor receptor (EGFR) inhibitor--induced skin disorder.
[061] The invention further provides methods of treating and/or preventing dermatologic toxicities induced by targeted therapy and/or immunotherapy comprising administering to a patient in need thereof a pharmaceutically effective amount of the pharmaceutical composition of the invention.
[062] In one embodiment, said targeted therapy is selected from the group consisting of EGFR, multityrosine kinase (MTK). MEK, phosphoinositide 3-kinase (PI3K), protein kinase B
(AKT), BRAF inhibitors. HER2 inhibitor, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC
inhibitors, proteasorne inhibitors, and RXR agonists; said immunotherapy is selected from the group consisting of cancer vaccines, cytokine agents (e.g., granulocyte-macrophage colony-stimulating factor (GM-CSF), interferons, and interleukin-2 (1-2)), cell therapies (e.g., tumor-infiltrating lymphocytes (TILs), T-cell receptor (TCR)-engineered peripheral blood lymphocytes (PBL), and chimeric antigen receptor (CAR)-engineered PBL), immune checkpoint protein inhibitors (e.g., PD-1, PD-L1, CTLA-4, TIM-3, LAG-3, BTLA, VISTA, and TIGIT), and immune checkpoint protein stimulators (e.g., CD28, ICOS, 4-1BB, 0X40, BITR, CD27, TWEAKR, HVEM, TIM-1, and CD-40); and said dermatologic toxicity induced by targeted therapy and/or immunotherapy is selected from the group consisting of papulopustular rash, maculopapular rash, erythema, telangiectasias flushing, paronychia and fissure, hair changes, xerosis, mucositis, pruritus, and hand-foot skin reaction.
(AKT), BRAF inhibitors. HER2 inhibitor, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC
inhibitors, proteasorne inhibitors, and RXR agonists; said immunotherapy is selected from the group consisting of cancer vaccines, cytokine agents (e.g., granulocyte-macrophage colony-stimulating factor (GM-CSF), interferons, and interleukin-2 (1-2)), cell therapies (e.g., tumor-infiltrating lymphocytes (TILs), T-cell receptor (TCR)-engineered peripheral blood lymphocytes (PBL), and chimeric antigen receptor (CAR)-engineered PBL), immune checkpoint protein inhibitors (e.g., PD-1, PD-L1, CTLA-4, TIM-3, LAG-3, BTLA, VISTA, and TIGIT), and immune checkpoint protein stimulators (e.g., CD28, ICOS, 4-1BB, 0X40, BITR, CD27, TWEAKR, HVEM, TIM-1, and CD-40); and said dermatologic toxicity induced by targeted therapy and/or immunotherapy is selected from the group consisting of papulopustular rash, maculopapular rash, erythema, telangiectasias flushing, paronychia and fissure, hair changes, xerosis, mucositis, pruritus, and hand-foot skin reaction.
[063] The concentrations of berberine in epidermis, dermis, and receiver (which refers to a container filled with PBS that in contact with the skin) are measured by the following approach.
Franz diffusion cell setup is essentially a piece of skin clamped between two clamps. The drug is applied on one side of the skin (top) and drug concentration is measured in the received portion (bottom) of the setup.
Franz diffusion cell setup is essentially a piece of skin clamped between two clamps. The drug is applied on one side of the skin (top) and drug concentration is measured in the received portion (bottom) of the setup.
[064] As used herein, the term "penetration rate" refers to an amount of berberine that presents in per gram of epidermis or dermis tissue, or an amount of berberine per cm2 of skin that presents in the receiver, after a certain time period from the application of a formulation to the skin.
[065] Amount of the drug measured in the receiver indicates the total amount that penetrated through SC, epidermis and dermis region of the skin. The pharmaceutical composition of the present invention has improved penetration rate, and the preferred range of the penetration rate is as follows:
Epidermis: 0.4 to 4000 pg of berberine per gram of tissue Dermis: 0.003 to 30 pg of berberine per gram of tissue Receiver: 0.0001 to 1 pg of berberine per 1X1 cm2 of skin.
Epidermis: 0.4 to 4000 pg of berberine per gram of tissue Dermis: 0.003 to 30 pg of berberine per gram of tissue Receiver: 0.0001 to 1 pg of berberine per 1X1 cm2 of skin.
[066] The following Table 1 lists various ingredients that may be used in the compositions of the invention. This list, however, is only provided for illustration purpose, but not to limit the scope of the present invention. Further, different ingredients/excipients can act in more than one way, e.g. can function as a penetration enhancer, an emulsifying agent, a wetting agent, etc.
Concentration Excipient Function Replacements/Analogs range ( /0) Anionic Sodium LaurylSulfate, Sulfonate, Phosphate, Oleate, penetration 0.5-2.5 Sulfate monostearate and Carboxylates enhancer Carbopol Carbopol 940, Carbopol 941, Carbopol Gelling agent 0.3-3 934P 971, Carbopol 974, Carbopol 980, Carbopol 981, Carbopol 5984EP, Carbopol ETD 2020, Hydroxyethyl Hydroxyethylmethyl cellulose, Gelling agent 0.5-8 Cellulose Hydroxypropyl cellulose, Hydroxypropyl cellulose (Low-substituted), Methyl cellulose, Methylhydroxypropyl cellulose Concentration Excipient Function Replacements/Analogs range (%) Quaternary ammonium compounds, Methylparaben 0.02-0.3 Amino aryl acid esters, Alkyl/Aryl alcohols, Alkyl/Aryl acids, Alkyl/Aryl Preservatives amides, Organomercurials, Propylparaben 0.01-0.8 Formaldehyde donators, Biguanides, Phenols Dipotassium edetate, Disodium edetate, EDTA Antioxidant 0.005-0.1 Edetate calcium disodium, Sodium edetate, Trisodium edetate Glycerin Humectant 0.01-30 Propylene glycol, Polyethylene glycol Tween 80 Wetting agent 0.1-3 Polyethylene glycol, Sorbitan esters Anhydrous citric acid, Fumaric acid, Citric acid 0.1-2 Malic acid, Sodium citrate dehydrate, monohydrate Buffering agent Tartaric acid Sodium citrate Anhydrous sodium citrate; citric acid 0.3-2 dihydrate monohydrate Water Water Calcium stearate, Magnesium stearate, Polyoxyethylene stearates, Purified Stearic acid Oil base 1-20 stearic acid, Zinc stearate, Lauric acid, Myristic acid, Palmitic acid, Oleic acid Mineral oil, Almond oil, Cocoa oil, Corn Castor oil Oil base 5-12.5 oil, Coconut oil, Cotton seed oil, Linseed oil, Olive oil, Soybean oil Yellow petrolatum, Liquid petrolatum, White Oil base 4-56 Paraffin, Ceresin, Microcrystalline wax, petrolatum Plastibase Polyoxyethylene sorbitan fatty acid SPAN 60 Emulsifying agent 1-15 esters, Tween 60 Emulsifying agent 1-15 Polyethylene glycol, Sorbitan esters i
Concentration Excipient Function Replacements/Analogs range ( /0) Anionic Sodium LaurylSulfate, Sulfonate, Phosphate, Oleate, penetration 0.5-2.5 Sulfate monostearate and Carboxylates enhancer Carbopol Carbopol 940, Carbopol 941, Carbopol Gelling agent 0.3-3 934P 971, Carbopol 974, Carbopol 980, Carbopol 981, Carbopol 5984EP, Carbopol ETD 2020, Hydroxyethyl Hydroxyethylmethyl cellulose, Gelling agent 0.5-8 Cellulose Hydroxypropyl cellulose, Hydroxypropyl cellulose (Low-substituted), Methyl cellulose, Methylhydroxypropyl cellulose Concentration Excipient Function Replacements/Analogs range (%) Quaternary ammonium compounds, Methylparaben 0.02-0.3 Amino aryl acid esters, Alkyl/Aryl alcohols, Alkyl/Aryl acids, Alkyl/Aryl Preservatives amides, Organomercurials, Propylparaben 0.01-0.8 Formaldehyde donators, Biguanides, Phenols Dipotassium edetate, Disodium edetate, EDTA Antioxidant 0.005-0.1 Edetate calcium disodium, Sodium edetate, Trisodium edetate Glycerin Humectant 0.01-30 Propylene glycol, Polyethylene glycol Tween 80 Wetting agent 0.1-3 Polyethylene glycol, Sorbitan esters Anhydrous citric acid, Fumaric acid, Citric acid 0.1-2 Malic acid, Sodium citrate dehydrate, monohydrate Buffering agent Tartaric acid Sodium citrate Anhydrous sodium citrate; citric acid 0.3-2 dihydrate monohydrate Water Water Calcium stearate, Magnesium stearate, Polyoxyethylene stearates, Purified Stearic acid Oil base 1-20 stearic acid, Zinc stearate, Lauric acid, Myristic acid, Palmitic acid, Oleic acid Mineral oil, Almond oil, Cocoa oil, Corn Castor oil Oil base 5-12.5 oil, Coconut oil, Cotton seed oil, Linseed oil, Olive oil, Soybean oil Yellow petrolatum, Liquid petrolatum, White Oil base 4-56 Paraffin, Ceresin, Microcrystalline wax, petrolatum Plastibase Polyoxyethylene sorbitan fatty acid SPAN 60 Emulsifying agent 1-15 esters, Tween 60 Emulsifying agent 1-15 Polyethylene glycol, Sorbitan esters i
[067] As used herein, the term "berberine" refers to 5,6-dihydro-9,10-dirnethoxybenzo(g)-1,3-benzodioxolo (5,6-a) quinolizinium.
The invention also contemplates the use of analogues of berberine which include but are not limited to jatrorrhizine, palmatine, coptisine, 9-demethylberberine, 9-demethylpalmatine, 13-hydroxyberberine, berberrubine, palmatrubine, 9-0-ethylberberrubine, 9-0-ethyl-13-ethylberberrubine, 13-methyldihydroberberine N-methyl salt, tetrahydroprotoberberines and N-methyl salts thereof, 9-lauroylberberrubine chloride, and pharmaceutically acceptable salts of all these compounds.
The invention also contemplates the use of analogues of berberine which include but are not limited to jatrorrhizine, palmatine, coptisine, 9-demethylberberine, 9-demethylpalmatine, 13-hydroxyberberine, berberrubine, palmatrubine, 9-0-ethylberberrubine, 9-0-ethyl-13-ethylberberrubine, 13-methyldihydroberberine N-methyl salt, tetrahydroprotoberberines and N-methyl salts thereof, 9-lauroylberberrubine chloride, and pharmaceutically acceptable salts of all these compounds.
[068] As used herein, the term "pharmaceutically acceptable salts" includes salts of acidic or basic groups. Examples of pharmaceutically acceptable salts include those derived from inorganic acids, such as hydrochloric, hydrobromic, nitric, carbonic, monohydrogencarbonic, phosphoric, monohydrogenphosphoric, dihydrogenphosphoric, sulfuric, monohydrogensulfuric, hydriodic, or phosphorous acids and the like, as well as the salts derived from relatively nontoxic organic acids, such as acetic; propionic; isobutyric; maleic; malonic;
benzoic; succinic; suberic;
fumaric; mandelic; phthalic; benzenesulfonic; toluenesulfonic, including p-toluenesulfonic, m-toluenesulfonic, and o-toluenesulfonic; citric; tartaric; methanesulfonic;
and the like. Also included are salts of amino acids such as arginate and the like, and salts of organic acids, such as glucuronic or galacturonic acids and the like.
benzoic; succinic; suberic;
fumaric; mandelic; phthalic; benzenesulfonic; toluenesulfonic, including p-toluenesulfonic, m-toluenesulfonic, and o-toluenesulfonic; citric; tartaric; methanesulfonic;
and the like. Also included are salts of amino acids such as arginate and the like, and salts of organic acids, such as glucuronic or galacturonic acids and the like.
[069] As used herein, the terms "treatment" and "treating" include inhibiting the disease or condition, causing a reduction in severity and/or frequency of symptoms, elimination of symptoms and/or underlying cause, prevention of the occurrence of symptoms and/or their underlying cause, ameliorating and/or improving a patient's condition. Thus, "treating" a patient with said compositions of the invention includes prevention of a particular disorder in a susceptible individual, as well as management of a clinically symptomatic individual to inhibit or cause regression of a disorder or disease, and maintenance of the current state and/or prevention of a progression of a disorder or disease. Treatment can include prophylaxis, therapy, or cure
[070] As used herein, the term "pharmaceutically effective amount" of the compounds and/or pharmaceutical compositions of the invention refers to a sufficient amount of the compound and/or composition to treat, inhibit, ameliorate or prevent various red face related skin disorders, including but not limited to, targeted therapy-induced dermatologic toxicities, at a reasonable benefit/risk ratio applicable to any medical treatment. It will be understood, however, that the total daily usage of the compounds and/or compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific effective dose level for any particular patient will depend upon a variety of factors, including the disorder being treated and the severity of the disorder; activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient;
the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed; and like factors well known in the medical arts.
For example, it is well within the skill of the art to start doses of the composition at levels lower than required to achieve the desired therapeutic effect and to gradually increase the dosage until the desired effect is achieved.
the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed; and like factors well known in the medical arts.
For example, it is well within the skill of the art to start doses of the composition at levels lower than required to achieve the desired therapeutic effect and to gradually increase the dosage until the desired effect is achieved.
[071] The pharmaceutical composition can further include a pharmaceutically acceptable carrier, and can be in solid or liquid form, including but not limited to, tablets, powders, capsules, pellets, solutions, suspensions, elixirs, emulsions, gels, creams, patch, or suppositories, including rectal and urethral suppositories.
[072] As used herein, the term "pharmaceutically acceptable carrier" refers to a pharmaceutically acceptable material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, solvent or encapsulating material. A pharmaceutically acceptable carrier is compatible with the other ingredients of the composition, with the mode of administration, and not injurious to the patient. A pharmaceutically acceptable carrier may be either aqueous or non-aqueous. Pharmaceutically acceptable carriers include gums, starches, sugars, cellulosic materials, and mixtures thereof. Some examples of materials which can serve as pharmaceutically-acceptable carriers include, but are not limited to: (a) sugars, such as lactose, glucose and sucrose; (b) starches, such as corn starch and potato starch; (c) cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; (d) powdered tragacanth; (e) malt; (f) gelatin; (g) talc; (h) excipients, such as cocoa butter and suppository waxes; (i) oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; (j) glycols, such as propylene glycol; (k) polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; (I) esters, such as ethyl oleate and ethyl laurate; (m) agar; (n) buffering agents, such as magnesium hydroxide, aluminum hydroxide, boric acid and sodium borate, and phosphate buffers; (o) alginic acid; (p) pyrogen-free water; (q) isotonic saline; (r) Ringer's solution; (s) ethyl alcohol; (t) phosphate buffer solutions; and (u) other non-toxic compatible substances suitable for use in pharmaceutical compositions.
[073] The compositions of the invention may be administered using any means known in the art, including but not limited to oral, nasal, parenteral, topical, transdermal, or rectal routes of administration. Preferably, the compositions are adapted for oral or topical administration. For example, the active ingredient of the composition can be formulated with suitable excipients for the preparation of tablets, capsules, pellets, troches, lozenges, solutions, powders or granules, suspensions, hard or soft capsules, patches and any other suitable forms.
[074] The invention also provides a method of treating and/or preventing dermatologic toxicities induced by targeted therapy and/or immunotherapy comprising administering to a patient in need thereof a pharmaceutically effective amount of berberine or a biologically equivalent analogue thereof.
[075] The invention further provides a method of treating and/or preventing dermatologic toxicities induced by targeted therapy and/or immunotherapy comprising topically applying to affected skin a pharmaceutically effective amount of a topical pharmaceutical composition comprising berberine or a biologically equivalent analogue thereof.
[076] In one embodiment, the topical pharmaceutical composition is in the form of a lotion, cream, ointment, paste, gel, spray, suspension, emulsion, foam, patch, powder and liniment.
[077] In one embodiment, the topical pharmaceutical composition comprises at least 0.02%
w/w, preferably about 0.1% to about 2% w/w of berberine or a biologically equivalent analogue thereof, wherein the amounts are by the total weight of the composition.
w/w, preferably about 0.1% to about 2% w/w of berberine or a biologically equivalent analogue thereof, wherein the amounts are by the total weight of the composition.
[078] In one embodiment, berberine or the biologically equivalent analogue of berberine is the primary pharmaceutically acceptable active component.
[079] In another embodiment, berberine or the biologically equivalent analogue of berberine is the only pharmaceutically acceptable active component.
[080] The following Examples demonstrate some aspects of the invention. The Examples are not meant to limit the invention in any way.
Example 1 Mouse Skin Penetration Study of Berberine Formulations
Example 1 Mouse Skin Penetration Study of Berberine Formulations
[081] The following six berberine formulations were compared: 08, 0.125%, 0.3%, G22, G23 and G24.
Formulations
Formulations
[082] The formulations were as follows:
C8 (a cream-based formulation):
Water Phase berberine (0.12%), Tween0 60 (1%), Glycerin (3%), methylparaben (0.1%), propylparaben (0.02%), NaOH (to adjust pH to 5.5), and EDTA (0.02%).
Oil Phase stearic acid (7.5%), castor oil (8%), white petrolatum (6%), and SPAN 60 (2%).
0.125% (gel-based formulation):
berberine (0.125%), ethanol (2.5%), glycerol (10%), phenoxyethanol (0.3%), carbomer.
0.3% (gel-based formulation):
berberine (0.3%), propylene glycol (9.25%), PEG 400 (5.03%), methylparaben (0.1%), propylparaben (0.02%), NaOH (0.4%), EDTA (0.02%), Carbomer 934P
(1%).
G22 (gel-based formulation):
berberine (0.1%), SDS (0.086%), glycerol (10%), Tween8 80 (0.5%), methylparaben (0.1%), propylparaben (0.02%), citric acid (0.033%), sodium citrate dihydrate (0.115%), NaOH, EDTA (0.02%), Carbomer 934P (0.3%), HEC
250 HHX (1.2%). Particle Size Distribution: 3.83/11.34/27.24 (in the format D10/D50/D90, where each value refers to the respective percentage of particles below the stated size, i.e. 10% of the particles are less than 3.83, and so on).
G23 (gel-based formulation):
berberine (0.1%), SDS (0.086%), glycerol (10%), Tween 80 (0.5%), methylparaben (0.1%), propylparaben (0.02%), citric acid (0.033%), sodium citrate dihydrate (0.115%), NaOH, EDTA (0.02%), Carbomer 934P (0.3%), HEC
250 HHX (1.2%). Particle Size Distribution: 1.45/2.85/9.30 G24 (gel-based formulation):
berberine (0.1%), SDS (0.043%), glycerol (10%), Tween 80 (0.5%), methylparaben (0.1%), propylparaben (0.02%), citric acid (0.033%), sodium citrate dihydrate (0.115%), NaOH, EDTA (0.02%), Carbomer 934P (0.3%), HEC
250 HHX (1.2%). Particle Size Distribution: 1.55/2.86/5.44
C8 (a cream-based formulation):
Water Phase berberine (0.12%), Tween0 60 (1%), Glycerin (3%), methylparaben (0.1%), propylparaben (0.02%), NaOH (to adjust pH to 5.5), and EDTA (0.02%).
Oil Phase stearic acid (7.5%), castor oil (8%), white petrolatum (6%), and SPAN 60 (2%).
0.125% (gel-based formulation):
berberine (0.125%), ethanol (2.5%), glycerol (10%), phenoxyethanol (0.3%), carbomer.
0.3% (gel-based formulation):
berberine (0.3%), propylene glycol (9.25%), PEG 400 (5.03%), methylparaben (0.1%), propylparaben (0.02%), NaOH (0.4%), EDTA (0.02%), Carbomer 934P
(1%).
G22 (gel-based formulation):
berberine (0.1%), SDS (0.086%), glycerol (10%), Tween8 80 (0.5%), methylparaben (0.1%), propylparaben (0.02%), citric acid (0.033%), sodium citrate dihydrate (0.115%), NaOH, EDTA (0.02%), Carbomer 934P (0.3%), HEC
250 HHX (1.2%). Particle Size Distribution: 3.83/11.34/27.24 (in the format D10/D50/D90, where each value refers to the respective percentage of particles below the stated size, i.e. 10% of the particles are less than 3.83, and so on).
G23 (gel-based formulation):
berberine (0.1%), SDS (0.086%), glycerol (10%), Tween 80 (0.5%), methylparaben (0.1%), propylparaben (0.02%), citric acid (0.033%), sodium citrate dihydrate (0.115%), NaOH, EDTA (0.02%), Carbomer 934P (0.3%), HEC
250 HHX (1.2%). Particle Size Distribution: 1.45/2.85/9.30 G24 (gel-based formulation):
berberine (0.1%), SDS (0.043%), glycerol (10%), Tween 80 (0.5%), methylparaben (0.1%), propylparaben (0.02%), citric acid (0.033%), sodium citrate dihydrate (0.115%), NaOH, EDTA (0.02%), Carbomer 934P (0.3%), HEC
250 HHX (1.2%). Particle Size Distribution: 1.55/2.86/5.44
[083] The particle size of G22, G23 and G24 formulations were determined as follows.
[084] Purified water was prepared, then berberine chloride, Tween 80 and sodium lauryl sulfate (SDS) were added. After well dispersed, the mixture was micronized.
After that, the particle size was measured by a diffraction analyzer.
Experimental Conditions
After that, the particle size was measured by a diffraction analyzer.
Experimental Conditions
(085] Mice were sacrificed by cervical dislocation. The full-thickness flank skin was removed and placed on the diffusion cell in contact with receptor phase, which was 0.01M PBS (pH 7.4 at 37 C). Buffers were pumped through the receiver compartment at a flow rate of 3 - 4 mL/h. 3000 of formulations were added onto the skin surface in the donor compartment.
Receiver solutions were collected at hour 0, 1, 2, 3, 4, 6, 8, 10, and 12 for HPLC analysis. Skin flux was calculated from slope of the linear part of the cumulative amount berberine chloride penetrated versus time curve.
Results
Receiver solutions were collected at hour 0, 1, 2, 3, 4, 6, 8, 10, and 12 for HPLC analysis. Skin flux was calculated from slope of the linear part of the cumulative amount berberine chloride penetrated versus time curve.
Results
[086] Figure 1 demonstrates a plot of cumulative berberine penetrated (ng/cm2) vs time for all 6 tested formulations. As one can see, C8 (cream formulation) and G23 (gel-based formulation) penetrated the best as compared to the other formulations. This was unexpected because theoretically, all six formulations should penetrate with a similar rate due to physical properties of berberine in water phase.
[087] Figure 2 demonstrates a plot of cumulative berberine penetrated (ng/cm2) vs time for three gel suspension formulations (G22, G23 and G24). As one can see, penetration rate is positively correlated to the penetration enhancer (SDS) but is negatively correlated to berberine size. G23 and G24 with berberine size of D90 less than 10 pm have higher penetration rate than G22 with D90 higher than 10 pm.
Example 2 Mini-pig Penetration Study of Berberine Formulations
Example 2 Mini-pig Penetration Study of Berberine Formulations
[088] The following berberine formulations were compared: 1) C8, G22, and G23; and 2) 0.125%, 0.30%, and G23.
[089] Skin: Mini-pig ( Lanyu pig or Lee sung pig) skin dermatomed to 700 .Lni with electrical resistance > 10 kO (Millicell-ERS, Millipore).
Penetration experiments:
Penetration experiments:
[090] Pig skin was placed on the diffusion cell with dermal side in contact with receptor phase, which was filled with PBS (pH 7.4 at 37 C). 20 I of formulations were added onto the skin surface in the donor compartment. After 8 hours, the residual formulation on the skin surface was removed using three dry cotton swabs. At the end of 12 and 24 hours of treatment with formulations, skin was dismounted from the diffusion cell, again skin surface was cleaned carefully with three water-soaked cotton swabs. 10 tape-strippings were employed to remove stratum corneum. The skin was then placed on glass disc and heat-separated into epidermis and dermis at 60 C water bath for 90 seconds. Both the separated epidermis and dermis were weighed and minced, and extracted with 0.5 ml diluent (1%H3PO4:CH3OH (1:1)).
The skin 'extracts were centrifuged at 14,500 rpm for 20 min. Berberine chloride concentrations in the receiver solutions and supernatants from skin extracts were determined by HPLC. Recovery of berberine chloride from skin was determined by spiking known amounts of the drug into skin tissues and processed as described above.
Results
The skin 'extracts were centrifuged at 14,500 rpm for 20 min. Berberine chloride concentrations in the receiver solutions and supernatants from skin extracts were determined by HPLC. Recovery of berberine chloride from skin was determined by spiking known amounts of the drug into skin tissues and processed as described above.
Results
[091] Table 2 summarizes the results of this experiment.
Table 2 Run 1 (Comparing C8, G22, G23) 12hr Epidermis (pg/g) Dermis (pg/g) Receiver (pg/cm2) 08 6.15 0.22 0.069 G22 7.55 0.14 0.0625 G23 11.99 0.09 0.0025 24hr Epidermis (pg/g) Dermis (pg/g) Receiver (pg/cm2) 08 14.675 0.2 0.029 G22 30.95 0.43 0.1905 G23 100.315 0.64 0.0285 Run 2 (Comparing 0.125%, 0.30%, G23) 12hr Epidermis (pg/g) Dermis (pg/g) Receiver (pg/cm2) 0.125% 11.67 0.14 0 0.30% 22.09 0.41 0 G23 16.68 0.1 0 24hr Epidermis (pg/g) Dermis (pg/g) Receiver (pg/cm2) 0.125% 18.74 0.19 0.01 0.30% 39.83 0.15 0 G23 35.75 0.27 0.009
Table 2 Run 1 (Comparing C8, G22, G23) 12hr Epidermis (pg/g) Dermis (pg/g) Receiver (pg/cm2) 08 6.15 0.22 0.069 G22 7.55 0.14 0.0625 G23 11.99 0.09 0.0025 24hr Epidermis (pg/g) Dermis (pg/g) Receiver (pg/cm2) 08 14.675 0.2 0.029 G22 30.95 0.43 0.1905 G23 100.315 0.64 0.0285 Run 2 (Comparing 0.125%, 0.30%, G23) 12hr Epidermis (pg/g) Dermis (pg/g) Receiver (pg/cm2) 0.125% 11.67 0.14 0 0.30% 22.09 0.41 0 G23 16.68 0.1 0 24hr Epidermis (pg/g) Dermis (pg/g) Receiver (pg/cm2) 0.125% 18.74 0.19 0.01 0.30% 39.83 0.15 0 G23 35.75 0.27 0.009
[092] The mini-pig skin penetration results indicate that: a) C8 (cream formulation) penetrates surprisingly well; b) formulations containing berberine particles continuously released over the 24 hours (G22, G23 vs 08, 0.125% and 0.3%); c) G22 & G23 (formulations containing berberine particles) retained more berberine in the epidermis and dermis after 24 hours as compared to 08 (cream formulation); d) formulations containing berberine particles penetrated better than formulations with berberine in solution (G23 vs 0.3%); e) G23 retained approximately the same amount of berberine in the epidermis and more berberine in the dermis after 24 hours as compared to the 0.3% formulation even though G23 contained only 0.1%
berberine; and f) compared with other penetration enhancers (ethanol and glycerol in the 0.125%
formulation and propylene glycol and PEG 400 in the 0.3% formulation), the addition of SDS in G23 resulted in enhanced penetration rate and increased local concentration of berberine in epidermis and dermis.
Example 3 syin. Biopsy Results from a Patient Treated by Topical Formulation Comprising Berberine
berberine; and f) compared with other penetration enhancers (ethanol and glycerol in the 0.125%
formulation and propylene glycol and PEG 400 in the 0.3% formulation), the addition of SDS in G23 resulted in enhanced penetration rate and increased local concentration of berberine in epidermis and dermis.
Example 3 syin. Biopsy Results from a Patient Treated by Topical Formulation Comprising Berberine
[093] The subject tested was a 56 year old male who received afatinib, an EGFR inhibitor, for treatment of non-small cell lung cancer (NSCLC). Upon receiving afatinib, the subject started applying topical gel of G23 formulation on one side of his face and vehicle gel (G23 with no berberine) on the other side once daily.
[094] Bilateral skin biopsies from nasolabial folds (both sides of the nose) were collected from the subject completing two-week topical treatment. Skin specimens were obtained by incisional biopsy measuring 1.0cm x 0.5cm, then histologically processed using hematoxylin and eosin (H&E) staining. Evaluation was performed by a trained dermatopathologist.
[095] The H&E staining results (Figures 3 & 4) show that follicular structure remains intact and there was no inflammatory cell infiltrate for skin area treated with G23 (Figure 3) while there was destruction of the follicular structure, profuse infiltration of inflammatory cells at the perifollicular region vacuolar change of the dermal-epidermal junction of follicular epithelium for skin treated with vehicle gel (Figure 4), indicating potential anti-inflammatory effect of berberine (G23) for treating EGFR inhibitor-associated skin toxicity.
Example 4 Facial Lesion Results from a Patient Treated by Topical Formulation Comprising Borberine
Example 4 Facial Lesion Results from a Patient Treated by Topical Formulation Comprising Borberine
[096] Subjects initiating afatinib inhibitors (EGFRI therapy) were enrolled to receive half face for G23 and the other half for vehicle gel (G23 with no berberine) during the 4-week treatment period. Subjects were assigned at a 1:1 ratio to determine the side of face for application of study medications. Subjects initiated study medications within 1 day before or after initiation of EGFRI therapy. Study medication was administered once daily (QD) at bedtime (HS) to the designated half of the face.
[097] Number of facial lesions (papules and pustules) were evaluated at weekly visits for four weeks. As shown in Figures 5 to 7, a clear trend was found that the number of lesions on the half face treated with G23 is significantly lower than that on the other half, indicating potential therapeutic effect of berberine for treating EGFR inhibitor- or other targeted therapy-induced or immunotherapy-induced dermatologic toxicities.
Claims (17)
1. A method of treating and/or preventing dermatologic toxicities induced by targeted therapy and/or immunotherapy comprising administering to a patient in need thereof a pharmaceutically effective amount of berberine or a biologically equivalent analogue thereof.
2. The method according to claim 1, wherein said targeted therapy is selected from the group consisting of EGFR inhibitors, multityrosine kinase (MTK) inhibitors, MEK inhibitors.
phosphoinositide 3-kinase (PI3K) inhibitors, protein kinase B (AKT) inhibitors, BRAF inhibitors, HER2 inhibitors, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC inhibitors, proteasome inhibitors, and RXR
agonists.
phosphoinositide 3-kinase (PI3K) inhibitors, protein kinase B (AKT) inhibitors, BRAF inhibitors, HER2 inhibitors, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC inhibitors, proteasome inhibitors, and RXR
agonists.
3. The method according to claim 1, wherein said immunotherapy is selected from the group consisting of cancer vaccines, cytokine agents, cell therapies, immune checkpoint protein inhibitors, and immune checkpoint protein stimulators.
4. The method according to claim 1, wherein said dermatologic toxicity is selected from the group consisting of papulopustular rash, maculopapular rash, erythema, telangiectasias flushing, paronychia and fissure, hair changes, xerosis, mucositis, pruritus, and hand-foot skin reaction.
5. The method according to claim 1, wherein the biologically equivalent analogue of berberine is selected from the group consisting of jatrorrhizine, palmatine, coptisine, 9-demethylberberine, 9-demethylpalmatine, 13-hydroyberberine, berberrubine, palmatrubine, 9-O-ethylberberrubine, 9-O-ethyl-13-ethylberberrubine, 13-methyldihydroberberine N-methyl salt, tetrahydroprotoberberines and N-methyl salts thereof, and 9-lauroylberberrubine chloride.
6. The method according to claim 1, wherein the biologically equivalent analogue of berberine is palmatine or coptisine.
7. A method of treating and/or preventing dermatologic toxicities induced by targeted therapy and/or immunotherapy comprising topically applying to affected skin a pharmaceutically effective amount of a topical pharmaceutical composition comprising berberine or a biologically equivalent analogue thereof.
8. The method according to claim 7, wherein said targeted therapy is selected from the group consisting of EGFR inhibitors, multityrosine kinase (MTK) inhibitors, MEK inhibitors, phosphoinositide 3-kinase (PI3K) inhibitors, protein kinase B (AKT) inhibitors, BRAF inhibitors, HER2 inhibitors, multikinase angiogenesis inhibitors, mTOR inhibitors, ALK/c-met inhibitors, multikinase Abl inhibitors, BTK inhibitors, HDAC inhibitors, proteasome inhibitors, and RXR
agonists.
agonists.
9. The method according to 7, wherein said immunotherapy is selected from the group consisting of cancer vaccines, cytokine agents, interferons, interleukin-2, cell therapies, immune checkpoint protein inhibitors, and immune checkpoint protein stimulators.
10. The method according to claim 7, wherein said dermatologic toxicity is selected from the group consisting of papulopustular rash, maculopapular rash, erythema, telangiectasias flushing, paronychia and fissure, hair changes, xerosis, mucositis, pruritus, and hand-foot skin reaction.
11. The method according to claim 7, wherein the biologically equivalent analogue of berberine is selected from the group consisting of jatrorrhizine, palmatine, coptisine, 9-demethylberberine, 9-demethylpalmatine, 13-hydroyberberine, berberrubine, palmatrubine, 9-O-ethylberberrubine, 9-O-ethyl-13-ethylberberrubine, 13-methyldihydroberberine N-methyl salt, tetrahydroprotoberberines and N-methyl salts thereof, and 9-lauroylberberrubine chloride.
12. The method according to claim 7, wherein the biologically equivalent analogue of berberine is palmatine or coptisine.
13. The method according to claim 7, wherein the topical pharmaceutical composition comprises at least 0.02% w/w of berberine or a biologically equivalent analogue thereof.
14. The method according to claim 7, wherein the topical pharmaceutical composition comprises about 0.1% to about 2% w/w of berberine or a biologically equivalent analogue thereof.
15. The method according to Claim 7, wherein berberine or the biologically equivalent analogue of berberine is the primary pharmaceutically acceptable active component.
16. The method according to Claim 7, wherein berberine or the biologically equivalent analogue of berberine is the only pharmaceutically acceptable active component.
17. The method according to Claim 7, wherein the topical pharmaceutical composition is in the form of a lotion, cream, ointment, paste, gel, spray, suspension, emulsion, foam, patch, powder and liniment.
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PCT/US2016/039180 WO2016210230A1 (en) | 2015-06-24 | 2016-06-24 | Therapeutic uses of berberine formulations |
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EP3782618A4 (en) | 2018-04-16 | 2022-01-26 | OnQuality Pharmaceuticals China Ltd. | Method for preventing or treating side effects of cancer therapy |
CN109999034A (en) * | 2019-02-11 | 2019-07-12 | 浙江理工大学 | Oncoprotein TNIK kinases targets natural small molecule inhibitor and its application |
CN109806264B (en) * | 2019-04-10 | 2021-04-02 | 江苏海钥医药科技有限公司 | Pharmaceutical composition and application thereof |
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US7404967B2 (en) * | 1994-12-21 | 2008-07-29 | Cosmederm, Inc. | Topical product formulations containing strontium for reducing skin irritation |
US5760096A (en) * | 1996-10-18 | 1998-06-02 | Thornfeldt; Carl R. | Potent penetration enhancers |
KR100258849B1 (en) * | 1998-04-24 | 2000-08-01 | 박원배 | Pharmaceutically useful protoberberine derivative and its salts |
EP1080719A3 (en) * | 1999-09-03 | 2001-04-25 | Active Organics Inc. | Methods for potentiation of efficacy of topical actives by mono-acyl-(lyso)-glycerophosholipids |
US6440465B1 (en) | 2000-05-01 | 2002-08-27 | Bioderm, Inc. | Topical composition for the treatment of psoriasis and related skin disorders |
US9211259B2 (en) * | 2002-11-29 | 2015-12-15 | Foamix Pharmaceuticals Ltd. | Antibiotic kit and composition and uses thereof |
US20040146539A1 (en) | 2003-01-24 | 2004-07-29 | Gupta Shyam K. | Topical Nutraceutical Compositions with Selective Body Slimming and Tone Firming Antiaging Benefits |
US7205006B2 (en) * | 2003-09-25 | 2007-04-17 | Prime Pharmaceutical Corporation | Mahonia aquifolium extract, extraction process and pharmaceutical composition containing the same |
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US20050158404A1 (en) | 2003-11-18 | 2005-07-21 | Goodless Dean R. | Composition and method for treatment of acne |
ES2600909T3 (en) * | 2009-06-30 | 2017-02-13 | Derman Biomedicine Co. Ltd. | Compositions containing berberine or analogues thereof to treat rosacea or skin disorders related to redness of the face |
TW201538156A (en) * | 2013-12-19 | 2015-10-16 | Twi Biotechnology Inc | Berberine formulations and uses thereof |
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