CA2906406C - Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes - Google Patents
Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes Download PDFInfo
- Publication number
- CA2906406C CA2906406C CA2906406A CA2906406A CA2906406C CA 2906406 C CA2906406 C CA 2906406C CA 2906406 A CA2906406 A CA 2906406A CA 2906406 A CA2906406 A CA 2906406A CA 2906406 C CA2906406 C CA 2906406C
- Authority
- CA
- Canada
- Prior art keywords
- fad2
- seq
- deletion
- nos
- allele
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
- A01H6/542—Glycine max [soybean]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Nutrition Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361790655P | 2013-03-15 | 2013-03-15 | |
US61/790,655 | 2013-03-15 | ||
US201361885213P | 2013-10-01 | 2013-10-01 | |
US61/885,213 | 2013-10-01 | ||
PCT/IB2014/059752 WO2014141147A1 (en) | 2013-03-15 | 2014-03-13 | Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2906406A1 CA2906406A1 (en) | 2014-09-18 |
CA2906406C true CA2906406C (en) | 2023-03-14 |
Family
ID=50543262
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2906406A Active CA2906406C (en) | 2013-03-15 | 2014-03-13 | Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP2966983B1 (zh) |
JP (1) | JP6591898B2 (zh) |
CN (1) | CN105246324B (zh) |
BR (1) | BR112015022778B1 (zh) |
CA (1) | CA2906406C (zh) |
WO (1) | WO2014141147A1 (zh) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
UA118090C2 (uk) | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
WO2017134601A1 (en) * | 2016-02-02 | 2017-08-10 | Cellectis | Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes |
KR102117110B1 (ko) * | 2016-12-22 | 2020-05-29 | 주식회사 툴젠 | Fad2 유전자 조작된 올레인산 강화 식물체 및 이의 제조 방법 |
CN108374007B (zh) * | 2018-02-03 | 2021-09-21 | 吉林省农业科学院 | 高油酸转基因大豆事件eb8072外源插入片段侧翼序列及其应用 |
WO2021055838A1 (en) * | 2019-09-20 | 2021-03-25 | Calyxt, Inc. | Low phytoestrogen soybean based on gene editing |
WO2021108248A1 (en) * | 2019-11-27 | 2021-06-03 | Calyxt, Inc. | Tal-effector nucleases for gene editing |
CN112680465A (zh) * | 2020-12-28 | 2021-04-20 | 浙江农林大学 | 用于改造大豆脂肪酸组分的基因突变体及其应用 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6414223B1 (en) * | 1998-08-03 | 2002-07-02 | Cargill, Incorporated | Plants, seeds and oils having an elevated total monounsaturated fatty acid content |
US6453242B1 (en) * | 1999-01-12 | 2002-09-17 | Sangamo Biosciences, Inc. | Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites |
AU2004208031B2 (en) * | 2003-01-28 | 2009-10-08 | Cellectis | Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof. |
CN100339481C (zh) * | 2005-03-17 | 2007-09-26 | 东北师范大学 | 应用基因沉默技术提高大豆和花生的种子油酸含量的方法 |
CA2765560C (en) * | 2009-06-22 | 2017-10-31 | Saga University | Mutation that increases the oleic acid content in soybean oil and responsible gene thereof |
CN103002727B (zh) * | 2009-07-08 | 2016-04-27 | 密苏里大学管委会 | 利用传统大豆育种技术培育高油酸大豆的方法 |
NZ599351A (en) * | 2009-10-22 | 2014-02-28 | Dow Agrosciences Llc | Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis |
CN102770539B (zh) | 2009-12-10 | 2016-08-03 | 明尼苏达大学董事会 | Tal效应子介导的dna修饰 |
AR085892A1 (es) * | 2011-01-14 | 2013-11-06 | Univ Missouri | Metodo para desarrollar soja con un contenido elevado de acido oleico usando tecnicas de desarrollo convencionales |
UA118090C2 (uk) * | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
-
2014
- 2014-03-13 BR BR112015022778-3A patent/BR112015022778B1/pt active IP Right Grant
- 2014-03-13 WO PCT/IB2014/059752 patent/WO2014141147A1/en active Application Filing
- 2014-03-13 EP EP14718749.6A patent/EP2966983B1/en active Active
- 2014-03-13 CN CN201480028282.4A patent/CN105246324B/zh active Active
- 2014-03-13 CA CA2906406A patent/CA2906406C/en active Active
- 2014-03-13 JP JP2015562512A patent/JP6591898B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
JP6591898B2 (ja) | 2019-10-16 |
CA2906406A1 (en) | 2014-09-18 |
BR112015022778A2 (pt) | 2017-11-07 |
CN105246324B (zh) | 2018-08-28 |
EP2966983B1 (en) | 2021-07-28 |
CN105246324A (zh) | 2016-01-13 |
WO2014141147A1 (en) | 2014-09-18 |
JP2016509865A (ja) | 2016-04-04 |
EP2966983A1 (en) | 2016-01-20 |
BR112015022778B1 (pt) | 2023-04-11 |
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Effective date: 20190311 |
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