CA2840683C - Toxine de nematodes axmi277, et procedes pour l'utilisation de celle-ci - Google Patents

Toxine de nematodes axmi277, et procedes pour l'utilisation de celle-ci Download PDF

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Publication number
CA2840683C
CA2840683C CA2840683A CA2840683A CA2840683C CA 2840683 C CA2840683 C CA 2840683C CA 2840683 A CA2840683 A CA 2840683A CA 2840683 A CA2840683 A CA 2840683A CA 2840683 C CA2840683 C CA 2840683C
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CA
Canada
Prior art keywords
nucleotide sequence
plant
polypeptide
amino acid
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2840683A
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English (en)
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CA2840683A1 (fr
Inventor
Candace Poutre
Theodore Kahn
Alissa ANTHONY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Agricultural Solutions Seed US LLC
Original Assignee
Athenix Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Athenix Corp filed Critical Athenix Corp
Publication of CA2840683A1 publication Critical patent/CA2840683A1/fr
Application granted granted Critical
Publication of CA2840683C publication Critical patent/CA2840683C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne des compositions et des procédés permettant de conférer une activité pesticide à des bactéries, à des plantes, à des cellules de plantes, à des tissus de plantes, et à des semences de plantes. L'invention propose ainsi des compositions comprenant une séquence codante pour un polypeptide de toxine. De telles séquences codantes peuvent être utilisées dans des constructions d'ADN ou dans des cassettes d'expression destinées à une transformation ou à une expression intervenant à l'intérieur des plantes et des bactéries. Les compositions comprennent également des bactéries, des plantes, des cellules de plantes, des tissus de plantes et semences de plantes, qui ont subi une telle transformation. L'invention concerne plus particulièrement des molécules isolées dans les acides nucléiques de la toxine. L'invention concerne également des séquences d'acides aminés correspondant aux polynucléotides ainsi que des anticorps se liant spécifiquement à ces séquences d'acides aminés. L'invention concerne enfin des molécules qui ont été isolées dans les acides nucléiques et qui comprennent des séquences de nucléotides codant la séquence d'acide aminé décrite dans SEQ ID NO:2 ou 3, ou la séquence de nucléotides proposée dans SEQ ID NO:1, ainsi que certains de leurs fragments ou variantes.
CA2840683A 2012-06-29 2012-06-29 Toxine de nematodes axmi277, et procedes pour l'utilisation de celle-ci Active CA2840683C (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2012/044751 WO2014003769A1 (fr) 2012-06-29 2012-06-29 Toxine de nématodes axm1277, et procédés pour l'utilisation de celle-ci

Publications (2)

Publication Number Publication Date
CA2840683A1 CA2840683A1 (fr) 2014-01-03
CA2840683C true CA2840683C (fr) 2020-03-10

Family

ID=46545471

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2840683A Active CA2840683C (fr) 2012-06-29 2012-06-29 Toxine de nematodes axmi277, et procedes pour l'utilisation de celle-ci

Country Status (4)

Country Link
CN (1) CN103998610B (fr)
CA (1) CA2840683C (fr)
UA (1) UA117731C2 (fr)
WO (1) WO2014003769A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA45638A (fr) 2015-08-07 2019-05-22 BASF Agricultural Solutions Seed US LLC Utilisation du promoteur de l'annexine pour améliorer le rendement de plantes soumises à des conditions de stress
CN112513033A (zh) 2018-06-04 2021-03-16 拜耳公司 除草活性的双环苯甲酰基吡唑

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4196265A (en) 1977-06-15 1980-04-01 The Wistar Institute Method of producing antibodies
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5039523A (en) 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
US5240842A (en) 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
AU642889B2 (en) 1989-07-11 1993-11-04 Biotechnology Research And Development Corporation Aerosol beam microinjector
CA2051562C (fr) 1990-10-12 2003-12-02 Jewel M. Payne Isolats de bacillus thuringiensis actifs contre les dipteres
TW261517B (fr) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5743477A (en) 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6468523B1 (en) 1998-11-02 2002-10-22 Monsanto Technology Llc Polypeptide compositions toxic to diabrotic insects, and methods of use
US6938976B2 (en) 1999-06-16 2005-09-06 Eastman Kodak Company Printer and method therefor adapted to sense data uniquely associated with a consumable loaded into the printer
AU1803601A (en) 1999-11-29 2001-06-04 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
DK2032598T3 (da) * 2006-06-14 2013-01-21 Athenix Corp Axmi-031, axmi-039, axmi-040 og axmi-049, en familie af deltaendotoksingener og fremgangsmåder til deres anvendelse
CA2702084C (fr) 2007-10-09 2017-07-25 Athenix Corp. Procedes informatiques de conception de gene synthetique
BRPI0917159A2 (pt) * 2008-08-25 2015-07-28 Down Agrosciences Llc Proteínas cry14 modificadas do bacillus trhuringiensis para o controle de nematódeos
EP2403948A1 (fr) * 2009-03-06 2012-01-11 Athenix Corporation Procédés et compositions de lutte contre des nuisibles de plantes

Also Published As

Publication number Publication date
CA2840683A1 (fr) 2014-01-03
CN103998610B (zh) 2020-03-17
CN103998610A (zh) 2014-08-20
UA117731C2 (uk) 2018-09-25
WO2014003769A1 (fr) 2014-01-03

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Effective date: 20170621