CA2834007C - Appareil et procedes de separation de sperme - Google Patents

Appareil et procedes de separation de sperme Download PDF

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Publication number
CA2834007C
CA2834007C CA2834007A CA2834007A CA2834007C CA 2834007 C CA2834007 C CA 2834007C CA 2834007 A CA2834007 A CA 2834007A CA 2834007 A CA2834007 A CA 2834007A CA 2834007 C CA2834007 C CA 2834007C
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Prior art keywords
sperm
reservoir
outlet
inlet
path
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CA2834007A
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CA2834007A1 (fr
Inventor
Reza Nosrati
Lise Marie Eamer
Marion Vollmer
David Sinton
Armand Zini
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University of Toronto
Royal Institution for the Advancement of Learning
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University of Toronto
Royal Institution for the Advancement of Learning
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Il est décrit diverses réalisations de méthodes et appareils servant à séparer du sperme, y compris des appareils qui comprennent un réservoir dentrée et de sortie ou lun ou lautre des éléments suivants : i) un ensemble radial de microconduits disposés entre les réservoirs dentrée et de sortie afin détablir une communication fluidique entre ces derniers et conduire le sperme motile vers lintérieur entre le réservoir dentrée et le réservoir de sortie; ii) au moins un microconduit disposé entre les réservoirs dentrée et de sortie afin détablir une communication fluidique entre ces derniers, lequel microconduit comprend un orifice dentrée situé à côté du réservoir de sortie et un croisement situé entre les orifices dentrée et de sortie du conduit servant à conduire une partie du sperme qui entre par lorifice dentrée voir le réservoir de sortie, et ce, en fonction de la motilité du sperme le long des murs. Les méthodes comprennent remplir un appareil de fluide intermédiaire, introduire le sperme dans un réservoir dentrée, puis récupérer le spermatozoïde séparé du sperme à partir dun réservoir de sortie.
CA2834007A 2013-11-19 2013-11-19 Appareil et procedes de separation de sperme Active CA2834007C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2834007A CA2834007C (fr) 2013-11-19 2013-11-19 Appareil et procedes de separation de sperme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA2834007A CA2834007C (fr) 2013-11-19 2013-11-19 Appareil et procedes de separation de sperme

Publications (2)

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CA2834007A1 CA2834007A1 (fr) 2015-05-19
CA2834007C true CA2834007C (fr) 2022-03-29

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CA2834007A Active CA2834007C (fr) 2013-11-19 2013-11-19 Appareil et procedes de separation de sperme

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR107746A1 (es) 2017-02-24 2018-05-30 Herberto Ernesto Hector Repetto Dispositivo y método de separación de células móviles
CN112662550B (zh) * 2020-10-16 2023-11-10 熹微(苏州)生物医药科技有限公司 一种从精液样本中分离精子的装置和方法

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Publication number Publication date
CA2834007A1 (fr) 2015-05-19

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Effective date: 20181116