CA2798777C - Formation de jonctions neuromusculaires dans un systeme defini - Google Patents

Formation de jonctions neuromusculaires dans un systeme defini Download PDF

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CA2798777C
CA2798777C CA2798777A CA2798777A CA2798777C CA 2798777 C CA2798777 C CA 2798777C CA 2798777 A CA2798777 A CA 2798777A CA 2798777 A CA2798777 A CA 2798777A CA 2798777 C CA2798777 C CA 2798777C
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human
culture
serum
muscle
motoneurons
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CA2798777A1 (fr
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James Hickman
Xiufang Guo
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University of Central Florida Research Foundation Inc UCFRF
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University of Central Florida Research Foundation Inc UCFRF
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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    • G01N33/5058—Neurological cells
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    • C12N2501/50—Cell markers; Cell surface determinants
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
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    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating
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    • G01N2500/00—Screening for compounds of potential therapeutic value
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Abstract

L'invention concerne un procédé de formation de jonctions neuromusculaires comprenant la formation de jonctions neuromusculaires fonctionnelles entre des motoneurones et des cellules musculaires par la mise en culture conjointe d'un ou plusieurs motoneurones humains et d'une ou plusieurs cellules musculaires humaines dans un milieu sensiblement exempt de sérum. Une jonction neuromusculaire synthétique de mammifère comprend un motoneurone humain lié de manière fonctionnelle à une cellule musculaire humaine dans un milieu sensiblement exempt de sérum. Un substrat artificiel peut être utilisé pour supporter la ou les jonctions neuromusculaires.
CA2798777A 2010-04-23 2011-05-06 Formation de jonctions neuromusculaires dans un systeme defini Active CA2798777C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/765,996 2010-04-23
US12/765,996 US8815584B1 (en) 2009-04-23 2010-04-23 Method of co-culturing mammalian muscle cells and motoneurons
PCT/US2011/035585 WO2011133985A1 (fr) 2010-04-23 2011-05-06 Formation de jonctions neuromusculaires dans un système défini

Publications (2)

Publication Number Publication Date
CA2798777A1 CA2798777A1 (fr) 2011-10-27
CA2798777C true CA2798777C (fr) 2016-09-06

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CA2798777A Active CA2798777C (fr) 2010-04-23 2011-05-06 Formation de jonctions neuromusculaires dans un systeme defini

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US (3) US8835168B2 (fr)
EP (1) EP2585171B1 (fr)
CA (1) CA2798777C (fr)
WO (1) WO2011133985A1 (fr)

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US9952204B2 (en) 2018-04-24
US20140349884A1 (en) 2014-11-27
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US20110250682A1 (en) 2011-10-13
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US8835168B2 (en) 2014-09-16
US20130115694A1 (en) 2013-05-09
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