CA2775231A1 - Systemes et procedes de mesure de traduction de proteines cibles dans des cellules - Google Patents

Systemes et procedes de mesure de traduction de proteines cibles dans des cellules Download PDF

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Publication number
CA2775231A1
CA2775231A1 CA2775231A CA2775231A CA2775231A1 CA 2775231 A1 CA2775231 A1 CA 2775231A1 CA 2775231 A CA2775231 A CA 2775231A CA 2775231 A CA2775231 A CA 2775231A CA 2775231 A1 CA2775231 A1 CA 2775231A1
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CA
Canada
Prior art keywords
trna
pair
protein
labeled
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2775231A
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English (en)
Other versions
CA2775231C (fr
Inventor
Zeev Smilansky
Barry S. Cooperman
Yale E. Goldman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anima Biotech Inc
Original Assignee
Anima Cell Metrology Inc
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Publication date
Application filed by Anima Cell Metrology Inc filed Critical Anima Cell Metrology Inc
Publication of CA2775231A1 publication Critical patent/CA2775231A1/fr
Application granted granted Critical
Publication of CA2775231C publication Critical patent/CA2775231C/fr
Active legal-status Critical Current
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2775231A 2009-09-24 2010-09-21 Systemes et procedes de mesure de traduction de proteines cibles dans des cellules Active CA2775231C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24531309P 2009-09-24 2009-09-24
US61/245,313 2009-09-24
PCT/IL2010/000792 WO2011036666A2 (fr) 2009-09-24 2010-09-21 Systèmes et procédés de mesure de traduction de protéines cibles dans des cellules

Publications (2)

Publication Number Publication Date
CA2775231A1 true CA2775231A1 (fr) 2011-03-31
CA2775231C CA2775231C (fr) 2018-06-05

Family

ID=43796313

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2775231A Active CA2775231C (fr) 2009-09-24 2010-09-21 Systemes et procedes de mesure de traduction de proteines cibles dans des cellules

Country Status (5)

Country Link
US (2) US9034576B2 (fr)
EP (1) EP2480890B1 (fr)
AU (1) AU2010299444B2 (fr)
CA (1) CA2775231C (fr)
WO (1) WO2011036666A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2567749C (fr) * 2004-05-26 2013-07-23 Anima Cell Metrology Procede d'evaluation de sequences de ribonucleotides
EP2164857B1 (fr) * 2007-06-22 2016-10-05 The Trustees Of The University Of Pennsylvania Marquage fluorescent d'arn de transfert et étude d'une synthèse de protéine
CA2702007C (fr) * 2007-10-09 2017-03-21 Anima Cell Metrology, Inc. Systemes et procedes pour mesurer une activite de translation dans des cellules viables
WO2017085718A1 (fr) 2015-11-17 2017-05-26 Anima Biotech Inc. Molécules d'arnt-aptamères fonctionnelles
WO2017105127A1 (fr) * 2015-12-17 2017-06-22 연세대학교 산학협력단 Procédé de marquage fluorescent de protéines cibles d'une efficacité élevée à l'aide de la méthionine fluorescente purifiée et un système de traduction de cellules eucaryotes
GB201600512D0 (en) * 2016-01-12 2016-02-24 Univ York Recombinant protein production
US10908072B2 (en) 2016-12-15 2021-02-02 The Board Of Regents Of The University Of Texas System Total internal reflection and transmission illumination fluorescence microscopy imaging system with improved background suppression
CN114134025B (zh) * 2021-11-19 2023-08-22 中国科学院长春光学精密机械与物理研究所 基因测序系统及其测序方法

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US5082767A (en) 1989-02-27 1992-01-21 Hatfield G Wesley Codon pair utilization
US5856928A (en) 1992-03-13 1999-01-05 Yan; Johnson F. Gene and protein representation, characterization and interpretation process
AU4769893A (en) 1992-07-17 1994-02-14 Ribozyme Pharmaceuticals, Inc. Method and reagent for treatment of animal diseases
JPH0793370A (ja) 1993-09-27 1995-04-07 Hitachi Device Eng Co Ltd 遺伝子データベース検索システム
DE4410655C2 (de) 1994-03-26 2001-06-28 Heiko Schwertner Verfahren und Vorrichtung zur schnellen kontinuierlichen Sequenzanalyse von verschiedenen linearen polymeren Substanzen nach einer einheitlichen Vorgehensweise
US5643722A (en) 1994-05-11 1997-07-01 Trustees Of Boston University Methods for the detection and isolation of proteins
US6210941B1 (en) 1997-06-27 2001-04-03 The Trustees Of Boston University Methods for the detection and isolation of proteins
US5777079A (en) 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5626058A (en) 1995-04-24 1997-05-06 Karpowich; John W. Rotary actuator for a bicycle brake
US6189013B1 (en) 1996-12-12 2001-02-13 Incyte Genomics, Inc. Project-based full length biomolecular sequence database
FR2786352B1 (fr) 1998-11-24 2004-10-22 Ar2I Sa Analyses Rech S Et Inn Appareil d'acquisition d'images par fluorescence et systeme d'imagerie comportant un tel appareil
US20040023874A1 (en) 2002-03-15 2004-02-05 Burgess Catherine E. Therapeutic polypeptides, nucleic acids encoding same, and methods of use
CA2311201A1 (fr) 1999-08-05 2001-02-05 Genset S.A. Sequences marqueurs exprimees et proteines humaines codees
US6306628B1 (en) 1999-08-25 2001-10-23 Ambergen, Incorporated Methods for the detection, analysis and isolation of Nascent proteins
WO2001016375A2 (fr) 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Sequençage de molecules d'acide nucleique a grande vitesseen parallele
FR2798143A1 (fr) 1999-09-08 2001-03-09 A2F Dispositif de lecture sequentielle rapide de polynucleotides
US20030044972A1 (en) 2000-03-17 2003-03-06 Zoran Ristic Maize chloroplast protein synthesis elongation factors and methods of use for same
CA2314398A1 (fr) * 2000-08-10 2002-02-10 Edward Shipwash Jeux ordonnes de microechantillons et microsystemes pour l'analyse d'acides amines et le sequencage de proteines
GB0111015D0 (en) 2001-05-04 2001-06-27 Norsk Hydro As Genetic material
US20050118151A1 (en) 2001-05-29 2005-06-02 Syddansk Universitet Proteins in diabetes proteome anlysis
US6432957B1 (en) 2001-06-29 2002-08-13 Kowa Co., Ltd. Piperazine derivative
US7065236B2 (en) 2001-09-19 2006-06-20 Tripath Imaging, Inc. Method for quantitative video-microscopy and associated system and computer software program product
US7741112B2 (en) 2001-12-13 2010-06-22 University Of Virginia Patent Foundation Vector mediated organelle transfection
US20030134423A1 (en) 2002-01-04 2003-07-17 Chu Yong Liang Compounds for delivering substances into cells
US7288372B2 (en) 2002-01-17 2007-10-30 Ambergen, Inc. Methods for the preparation of chemically misaminoacylated tRNA via protective groups
US20040023256A1 (en) * 2002-01-25 2004-02-05 Puglisi Joseph D. Translation profiling
AU2003209403A1 (en) 2002-01-25 2003-09-02 The Board Of Trustees Of The Leland Stanford Junior University Surface based translation system
CA2504933C (fr) * 2002-11-06 2012-10-16 Dana-Farber Cancer Institute, Inc. Procedes et compositions de traitement du cancer au moyen d'inhibiteurs de la proteasome
EP1578969B1 (fr) 2002-11-08 2010-04-07 The University Of Queensland Procede d'optimisation de l'expression genique au moyen d'une optimisation de codon synonyme
AU2003283791B2 (en) 2002-11-29 2010-04-22 Anima Cell Metrology Protein synthesis monitoring (PSM)
US8442773B2 (en) 2002-11-29 2013-05-14 Anima Cell Metrology Protein synthesis monitoring (PSM)
WO2005001062A2 (fr) 2003-06-25 2005-01-06 Gencia Corporation Vecteurs modifies pour la transfection d'organite
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CA2567749C (fr) * 2004-05-26 2013-07-23 Anima Cell Metrology Procede d'evaluation de sequences de ribonucleotides
WO2007002758A2 (fr) 2005-06-24 2007-01-04 Biolase Technology, Inc. Systemes de retour d'informations visuelles pour dispositifs de production d'energie electromagnetique
US20070134814A1 (en) 2005-12-09 2007-06-14 Kajander E O Methods and compositions for the detection of calcifying nano-particles, identification and quantification of associated proteins thereon, and correlation to disease
CA2662893A1 (fr) 2006-09-06 2008-03-13 University Health Network Quantification de fluorescence et acquisition d'image dans un support extremement trouble
EP2164857B1 (fr) 2007-06-22 2016-10-05 The Trustees Of The University Of Pennsylvania Marquage fluorescent d'arn de transfert et étude d'une synthèse de protéine
CA2702007C (fr) 2007-10-09 2017-03-21 Anima Cell Metrology, Inc. Systemes et procedes pour mesurer une activite de translation dans des cellules viables

Also Published As

Publication number Publication date
US20150293023A1 (en) 2015-10-15
AU2010299444B2 (en) 2015-12-10
AU2010299444A1 (en) 2012-05-03
WO2011036666A2 (fr) 2011-03-31
WO2011036666A3 (fr) 2011-10-13
US9034576B2 (en) 2015-05-19
CA2775231C (fr) 2018-06-05
US20120183957A1 (en) 2012-07-19
EP2480890A4 (fr) 2013-08-28
EP2480890A2 (fr) 2012-08-01
EP2480890B1 (fr) 2018-02-28

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Effective date: 20150729