CA2769420A1 - Proteases with modified pre-pro regions - Google Patents

Proteases with modified pre-pro regions Download PDF

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Publication number
CA2769420A1
CA2769420A1 CA2769420A CA2769420A CA2769420A1 CA 2769420 A1 CA2769420 A1 CA 2769420A1 CA 2769420 A CA2769420 A CA 2769420A CA 2769420 A CA2769420 A CA 2769420A CA 2769420 A1 CA2769420 A1 CA 2769420A1
Authority
CA
Canada
Prior art keywords
protease
polynucleotide
seq
amino acid
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2769420A
Other languages
English (en)
French (fr)
Inventor
Alexander Pisarchik
Brian F. Schmidt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of CA2769420A1 publication Critical patent/CA2769420A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2769420A 2009-07-31 2010-04-15 Proteases with modified pre-pro regions Abandoned CA2769420A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23024709P 2009-07-31 2009-07-31
US61/230,247 2009-07-31
PCT/US2010/031283 WO2011014278A1 (en) 2009-07-31 2010-04-15 Proteases with modified pre-pro regions

Publications (1)

Publication Number Publication Date
CA2769420A1 true CA2769420A1 (en) 2011-02-03

Family

ID=42342462

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2769420A Abandoned CA2769420A1 (en) 2009-07-31 2010-04-15 Proteases with modified pre-pro regions

Country Status (9)

Country Link
US (1) US20110171718A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP2459714A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JP5852568B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (1) CN102575242B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AR (1) AR076311A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BR (1) BR112012002163A2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA2769420A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2012DN00312A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2011014278A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140329309A1 (en) 2011-12-09 2014-11-06 Danisco Us Inc. Ribosomal Promoters for Production in Microorganisms
GB201212932D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
GB201212934D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
DK3197472T3 (da) * 2014-09-22 2021-10-18 Tanea Medical Ab Rekombinante phe-frie proteiner til anvendelse i behandlingen af phenylketonuri
WO2016134213A2 (en) 2015-02-19 2016-08-25 Danisco Us Inc Enhanced protein expression
US10731144B2 (en) 2015-06-17 2020-08-04 Danisco Us Inc Proteases with modified propeptide regions
JP7103948B2 (ja) 2016-03-04 2022-07-20 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター
DE102016204814A1 (de) 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Verbesserte Reinigungsleistung an Protein sensitiven Anschmutzungen
DE102016204815A1 (de) 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Proteasen mit verbesserte Enzymstabilität in Waschmittel
DE102016208463A1 (de) * 2016-05-18 2017-11-23 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen
WO2017210295A1 (en) * 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
CN110846299B (zh) * 2019-11-22 2021-09-24 江南大学 一种前导肽突变体及其在角蛋白酶生产中的应用
WO2021230358A1 (ja) * 2020-05-15 2021-11-18 Jnc株式会社 抗ウイルス剤
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2025034713A2 (en) 2023-08-09 2025-02-13 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310675A (en) * 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US5431382A (en) * 1994-01-19 1995-07-11 Design Technology Corporation Fabric panel feed system
US7101698B2 (en) * 2002-06-26 2006-09-05 Kao Corporation Alkaline protease
CN101597601B (zh) * 2002-06-26 2013-06-05 诺维信公司 具有改变的免疫原性的枯草杆菌酶和枯草杆菌酶变体
JP4210548B2 (ja) * 2002-06-26 2009-01-21 花王株式会社 アルカリプロテアーゼ
US20080020440A1 (en) * 2002-08-27 2008-01-24 Daniel Tillett Method of sequestering and/or purifying a polypeptide
US7807174B2 (en) * 2002-11-22 2010-10-05 Nexbio, Inc. Class of therapeutic protein based molecules
US7655242B2 (en) * 2002-12-13 2010-02-02 Case Western Reserve University Defensin-inducing agents
US7490416B2 (en) * 2004-01-26 2009-02-17 Townsend Herbert E Shoe with cushioning and speed enhancement midsole components and method for construction thereof
WO2008112258A2 (en) * 2007-03-12 2008-09-18 Danisco Us Inc. Modified proteases
US20100297727A1 (en) * 2007-06-06 2010-11-25 Wolfgang Aehle Methods for Improving Protein Performance
AR076312A1 (es) * 2009-04-24 2011-06-01 Danisco Us Inc Proteasas con regiones pro modificadas

Also Published As

Publication number Publication date
CN102575242B (zh) 2015-03-25
WO2011014278A1 (en) 2011-02-03
AR076311A1 (es) 2011-06-01
EP2459714A1 (en) 2012-06-06
BR112012002163A2 (pt) 2015-11-03
IN2012DN00312A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2015-05-08
US20110171718A1 (en) 2011-07-14
CN102575242A (zh) 2012-07-11
JP2013500714A (ja) 2013-01-10
JP5852568B2 (ja) 2016-02-03

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150413

FZDE Dead

Effective date: 20170906