CA2760941C - Systeme et procede destines a suivre le battement, la viabilite et la morphologie des cardiomyocytes et a cribler des molecules a la recherche d'agents pharmacologiques qui peuven t induire une cardiotoxicite ou moduler la fonction des cardiomyocytes - Google Patents
Systeme et procede destines a suivre le battement, la viabilite et la morphologie des cardiomyocytes et a cribler des molecules a la recherche d'agents pharmacologiques qui peuven t induire une cardiotoxicite ou moduler la fonction des cardiomyocytes Download PDFInfo
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
Abstract
La présente invention concerne des dispositifs et des procédés pour effectuer un enregistrement extracellulaire de cellules, telles que des cellules pouvant être excitées, des cardiomyocytes, et des cellules précurseurs de cardiomyocytes. Un dispositif cité en exemple comprend un substrat non conducteur formant une base ou fourni comme base d'un ou plusieurs puits ; une électrode d'enregistrement positionnée sur le substrat à l'intérieur du puits, l'électrode d'enregistrement étant accessible aux cellules lorsqu'un échantillon de cellules est ajouté au dispositif ; et une électrode de référence positionnée à l'intérieur du puits dans une zone exempte de cellules, la zone exempte de cellules étant caractérisée en ce qu'elle n'est pas en contact avec les cellules lorsque l'échantillon de cellules est ajouté au dispositif, empêchant ainsi tout contact entre les cellules et l'électrode de référence.
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17556609P | 2009-05-05 | 2009-05-05 | |
| US61/175,566 | 2009-05-05 | ||
| US31055710P | 2010-03-04 | 2010-03-04 | |
| US61/310,557 | 2010-03-04 | ||
| US32378210P | 2010-04-13 | 2010-04-13 | |
| US61/323,782 | 2010-04-13 | ||
| PCT/US2010/033801 WO2010129725A1 (fr) | 2009-05-05 | 2010-05-05 | Système et procédé destinés à suivre le battement, la viabilité et la morphologie des cardiomyocytes et à cribler des molécules à la recherche d'agents pharmacologiques qui peuvent induire une cardiotoxicité ou moduler la fonction des cardiomyocytes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2760941A1 CA2760941A1 (fr) | 2010-11-11 |
| CA2760941C true CA2760941C (fr) | 2019-07-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2760941A Active CA2760941C (fr) | 2009-05-05 | 2010-05-05 | Systeme et procede destines a suivre le battement, la viabilite et la morphologie des cardiomyocytes et a cribler des molecules a la recherche d'agents pharmacologiques qui peuven t induire une cardiotoxicite ou moduler la fonction des cardiomyocytes |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP2427543A4 (fr) |
| CA (1) | CA2760941C (fr) |
| WO (1) | WO2010129725A1 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US8263375B2 (en) | 2002-12-20 | 2012-09-11 | Acea Biosciences | Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology |
| US11346797B2 (en) | 2002-12-20 | 2022-05-31 | Agilent Technologies, Inc. | System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties |
| US9612234B2 (en) | 2008-05-05 | 2017-04-04 | Acea Biosciences, Inc. | Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (RTCA) cardio instruments |
| US10215748B2 (en) | 2002-12-20 | 2019-02-26 | Acea Biosciences, Inc. | Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways |
| US10539523B2 (en) | 2002-12-20 | 2020-01-21 | Acea Biosciences, Inc. | System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties |
| US10551371B2 (en) | 2003-11-10 | 2020-02-04 | Acea Biosciences, Inc. | System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function |
| EP1692258A4 (fr) | 2003-11-12 | 2007-03-21 | Xiao Xu | Systemes de detection de cellules electroniques en temps reel pour des epreuves a base de cellules |
| CA2723223C (fr) | 2008-05-05 | 2017-06-06 | Acea Biosciences, Inc. | Surveillance sans marqueur d'un couplage excitation-contraction et cellules pouvant etre excitees utilisant des systemes fondes sur l'impedance avec une resolution dans le temps de l'ordre de la milliseconde |
| WO2012178095A1 (fr) * | 2011-06-22 | 2012-12-27 | Ifluidics, Inc. | Système électromoteur pour le criblage à haut débit |
| EP2929014A4 (fr) | 2012-12-07 | 2016-08-03 | Univ Toronto | Constructions de tissu cardiaque et leurs procédés de fabrication |
| WO2018161063A1 (fr) | 2017-03-03 | 2018-09-07 | Acea Biosciences, Inc. | Procédés et systèmes pour la maturation fonctionnelle de cardiomyocytes dérivés de cspi et de cellule se |
| US11492609B2 (en) | 2017-08-16 | 2022-11-08 | Amgen Inc. | Adaptive electrode arrangement and method |
| CN113655102A (zh) * | 2021-08-25 | 2021-11-16 | 深圳市溢鑫科技研发有限公司 | 一种电化学测量微电极结构及其制作方法 |
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| US5981268A (en) * | 1997-05-30 | 1999-11-09 | Board Of Trustees, Leland Stanford, Jr. University | Hybrid biosensors |
| JPH11187865A (ja) * | 1997-12-25 | 1999-07-13 | Matsushita Electric Ind Co Ltd | 細胞電位測定電極及びこれを用いた測定装置 |
| US8206903B2 (en) * | 2002-12-20 | 2012-06-26 | Acea Biosciences | Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses |
| DE60329187D1 (de) * | 2002-07-20 | 2009-10-22 | Acea Biosciences Inc | Impedanz basierende vorrichtungen und verfahren zur analyse von zellen und partikeln |
| GB0414546D0 (en) * | 2004-06-29 | 2004-08-04 | Oxford Biosensors Ltd | Electrode for electrochemical sensor |
| WO2009038079A1 (fr) * | 2007-09-18 | 2009-03-26 | Biotec Co., Ltd. | Récipient de mesure de cellule, procédé de mesure de potentiel extracellulaire et procédé de test chimique |
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- 2010-05-05 EP EP10772804.0A patent/EP2427543A4/fr active Pending
- 2010-05-05 WO PCT/US2010/033801 patent/WO2010129725A1/fr active Application Filing
- 2010-05-05 CA CA2760941A patent/CA2760941C/fr active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2427543A4 (fr) | 2017-11-29 |
| CA2760941A1 (fr) | 2010-11-11 |
| EP2427543A1 (fr) | 2012-03-14 |
| WO2010129725A1 (fr) | 2010-11-11 |
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Effective date: 20150501 |