CA2751660A1 - A particle differentiation apparatus for spermatozoa - Google Patents
A particle differentiation apparatus for spermatozoa Download PDFInfo
- Publication number
- CA2751660A1 CA2751660A1 CA2751660A CA2751660A CA2751660A1 CA 2751660 A1 CA2751660 A1 CA 2751660A1 CA 2751660 A CA2751660 A CA 2751660A CA 2751660 A CA2751660 A CA 2751660A CA 2751660 A1 CA2751660 A1 CA 2751660A1
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- CA
- Canada
- Prior art keywords
- per
- particle
- particle differentiation
- differentiation apparatus
- sperm cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
Isolated non-naturally occurring populations of spermatozoa (15) having high purity and technologies to differentiate spermatozoa (28) based on characteristics such as mass, volume, orientation, or emitted light including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32).
Claims (27)
1. A particle differentiation apparatus, comprising:
a. asymmetric particles b. an irradiation source generating an irradiation beam responsive to said asymmetric particles;
c. optics to focus said irradiation beam responsive to said asymmetrical particles, wherein said optics focus a beam pattern having a height of about equal to the length of said asymmetrical particles along the longitudinal axis to about three times the length of said asymmetrical particles along the longitudinal axis;
d. a light emission material coupled to said asymmetric particles, wherein said light emission material emits light in response to said irradiation beam;
e. a detector responsive to said light.
a. asymmetric particles b. an irradiation source generating an irradiation beam responsive to said asymmetric particles;
c. optics to focus said irradiation beam responsive to said asymmetrical particles, wherein said optics focus a beam pattern having a height of about equal to the length of said asymmetrical particles along the longitudinal axis to about three times the length of said asymmetrical particles along the longitudinal axis;
d. a light emission material coupled to said asymmetric particles, wherein said light emission material emits light in response to said irradiation beam;
e. a detector responsive to said light.
2. A particles differentiation apparatus as described in claim 1, wherein said at least one asymmetrical particle comprise at least one sperm cell.
3. A particle differentiation apparatus as described in claim 2, wherein said at least one sperm cell has a head having a length along the longitudinal axis of about nine micrometers, and wherein said irradiation pattern has a height of about 20 micrometers.
4. A particle differentiation apparatus as described in claim 1, wherein said at least one asymmetric particle has at least one particle differentiation characteristic.
5. A particle differentiation apparatus as described in claim 4, further comprising a fluid stream.
6. A particle differentiation apparatus as described in claim 5, wherein said at least one particle differentiation characteristic comprises orientation of said asymmetric particle within said fluid stream, and wherein said detector is differentially responsive to said light emitted from said light emission material based said particle orientation characteristics.
7. A particle differentiation apparatus as described in claim 6, further comprising an analyzer coupled to said detector.
8. A particle differentiation apparatus as described in claim 7, wherein said analyzer differentiates between said asymmetric particles based upon said orientation of said asymmetric particle within said fluid stream.
9. A particle differentiation apparatus as described in claim 8, wherin said light emission material bound to said at least one asymmetric particle comprises a stain bound to the nuclear DNA of sperm cells.
10. A particle differentiation apparatus as described in claim 9, wherein said particle differentiation characteristic comprises a difference in amount of said stain bound the nuclear DNA of X-chromosome sperm cells and the nuclear DNA of Y-chromosome bearing sperm cells.
11. A particle differentiation apparatus as described in claim 10, wherein said detector comprises at least one photomultiplier tube, and wherein said photomultiplier tube has a typical operation voltage range, and wherein said photomultiplier tube is operated outside said typical operation voltage range.
12. A particle differentiation apparatus as described in claim 11, wherein said typical operation voltage range of said photomultiplier tube is from about 400 volts to about 999 volts.
13. A particle differentiation apparatus as described in claim 12, wherein said photomultiplier tube is operated in a range of from about 0 volts to about 300 volts.
14. A particle differentiation apparatus as descried in claim 11, further comprising droplets breaking off from said fluid stream each having one of said particles entrained.
15. A particle differentiation apparatus as described in claim 14, wherein said droplets breaking off from said fluid stream have sufficient size to encapsulate said one of said sperm cells, wherein said sperm cells comprise intact live sperm cells having at least a head and a neck and a tail.
16. A particle differentiation apparatus as described in claim 15, further comprising a nozzle having an orifice of about 100 micrometers in diameter.
17. A particle differentiation apparatus as described in claim 14, further comprising a droplet charger coupled to said analyzer, wherein said droplets receive a charge differentially based upon said difference in amount of said stain bound the nuclear DNA of X-chromosome sperm cells and the nuclear DNA of Y-chromosomes bearing sperm cells.
18. A particle differentiation apparatus as described in claim 17, further comprising a droplet separator, wherein said droplet separator separates said droplet based upon charge of said droplet.
19. A particle differentiation apparatus as described in claim 18, further comprising at least one collection container in which droplets containing said X-chromosome bearing sperm cells are collected as an X-chromosome bearing population.
20. A particle differentiation apparatus as described in claim 18, further comprising at least one collection container in which droplets containing Y-chromosome bearing sperm cells are collected as a Y-chromosome bearing population.
21. A particle differentiation apparatus as described in claims 19 or 20 wherein said X-chromosome bearing population and said Y-chromosome bearing population of said sperm cells are selected from the group consisting of between 90% to about 100%, between about 91% to about 100%, between about 92% to about 100%, between about 93% to about 100%, between about 94% to about 100%, between about 95% to about 100%, between about 96% to about 100%, between about 97% to about 100%, between about 98% to about 100%, between about 99% to about 100%.
22. A particle differentiation apparatus as described inclaim 21, further comprising the step of establishing a separable event rate wherein said separable event rate is selected from the group consisting of at least 5000 separable events per second, at least 6000 separable events per second, at least 7000 separable events per second, at least 8000 separable events per second, at least 9000 separable events per second, at least 10,000 separable events per second, at least 11,000 separable events per second, at least 12,000 separable events per second, at least 13000 separable events per second, at least 14,000 separable events per second, at least 16,000 separable events per second, at least 17,000 separable events per second, at least 18,000 separable events per second, at least 19,000 separable events per second, at least 20,000 separable events per second, at least 21,000 separable events per second.
23. An apparatus for differentiating particles as described in claim 22, wherein said step of separating said sperm cells comprises a separation rate selected from the group consisting of at least 500 separations per second, at least 1,000 separations per second, at least 2,000 separations per second, at least 3,000 separations per second, at least 4,000 separations per second, at least 5,000 separations per second, at least 6,000 separations per second, at least 7,000 separations per second, at least 8,000 separations per second, at least 9,000 separations per second, at least 10,000 separations per second, at least 11,000 separations per second.
24. An apparatus for differentiating particles as described in claim 23, wherein said step of forming droplets each having one of said sperm cells entrained comprises a droplet formation rate selected from the group consisting of at least 10,000 droplets per second, at least 20, 000 droplets per second, at least 30, 000 droplets per second, at least 40, 000 droplets per second, at least 50, 000 droplets per second, at least 60, 000 droplets per second, at least 70, 000 droplets per second, at least 80, 000 droplets per second, at least 90, 000 droplets per second, at least 100, 000 droplets per second.
25. A particle differentiation apparatus as described in claim 24, wherein said sperm cell comprise a bovine sperm cells.
26. A particle differentiation apparatus as described in claim 24, wherein said sperm cells comprises sperm cells from an equine mammal.
27. A particle differentiation apparatus as described in claim 24, wherein said sperm cells comprise sperm cells from an ovine mammal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2803774A CA2803774C (en) | 2000-05-09 | 2001-05-09 | A particle differentiation apparatus for spermatozoa |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20308900P | 2000-05-09 | 2000-05-09 | |
| US60/203,089 | 2000-05-09 | ||
| US23975200P | 2000-10-12 | 2000-10-12 | |
| US60/239,752 | 2000-10-12 | ||
| US26757101P | 2001-02-10 | 2001-02-10 | |
| US60/267,571 | 2001-02-10 | ||
| CA2408939A CA2408939C (en) | 2000-05-09 | 2001-05-09 | High purity x-chromosome bearing and y-chromosome bearing populations of spermatozoa |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2408939A Division CA2408939C (en) | 2000-05-09 | 2001-05-09 | High purity x-chromosome bearing and y-chromosome bearing populations of spermatozoa |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2803774A Division CA2803774C (en) | 2000-05-09 | 2001-05-09 | A particle differentiation apparatus for spermatozoa |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2751660A1 true CA2751660A1 (en) | 2001-11-15 |
| CA2751660C CA2751660C (en) | 2013-08-20 |
Family
ID=44789655
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2803774A Expired - Lifetime CA2803774C (en) | 2000-05-09 | 2001-05-09 | A particle differentiation apparatus for spermatozoa |
| CA2751660A Expired - Lifetime CA2751660C (en) | 2000-05-09 | 2001-05-09 | A particle differentiation apparatus for spermatozoa |
| CA2858892A Expired - Lifetime CA2858892C (en) | 2000-05-09 | 2001-05-09 | A particle differentiation apparatus for spermatozoa |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2803774A Expired - Lifetime CA2803774C (en) | 2000-05-09 | 2001-05-09 | A particle differentiation apparatus for spermatozoa |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2858892A Expired - Lifetime CA2858892C (en) | 2000-05-09 | 2001-05-09 | A particle differentiation apparatus for spermatozoa |
Country Status (1)
| Country | Link |
|---|---|
| CA (3) | CA2803774C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102830053A (en) * | 2003-03-28 | 2012-12-19 | 英格朗公司 | Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm |
-
2001
- 2001-05-09 CA CA2803774A patent/CA2803774C/en not_active Expired - Lifetime
- 2001-05-09 CA CA2751660A patent/CA2751660C/en not_active Expired - Lifetime
- 2001-05-09 CA CA2858892A patent/CA2858892C/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102830053A (en) * | 2003-03-28 | 2012-12-19 | 英格朗公司 | Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm |
| US9040304B2 (en) | 2003-03-28 | 2015-05-26 | Inguran, Llc | Multi-channel system and methods for sorting particles |
| US9377390B2 (en) | 2003-03-28 | 2016-06-28 | Inguran, Llc | Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm |
| US10100278B2 (en) | 2003-03-28 | 2018-10-16 | Inguran, Llc | Multi-channel system and methods for sorting particles |
| US11104880B2 (en) | 2003-03-28 | 2021-08-31 | Inguran, Llc | Photo-damage system for sorting particles |
| US11718826B2 (en) | 2003-03-28 | 2023-08-08 | Inguran, Llc | System and method for sorting particles |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2858892A1 (en) | 2001-11-15 |
| CA2803774C (en) | 2014-09-30 |
| CA2858892C (en) | 2019-06-11 |
| CA2803774A1 (en) | 2001-11-15 |
| CA2751660C (en) | 2013-08-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20210510 |