CA2723950C - Procedes et reactifs pour la determination de la longueur de telomeres d'une maniere semi-automatique de chaque cellule individuelle dans une population de cellules immobilisees - Google Patents
Procedes et reactifs pour la determination de la longueur de telomeres d'une maniere semi-automatique de chaque cellule individuelle dans une population de cellules immobilisees Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
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- Biotechnology (AREA)
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- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention porte sur des procédés et des réactifs pour la détermination de la longueur de télomères dans des sections de tissu par la technique de cartographie télomérique des cellules individuelles basée sur une étape d'hybridation in situ fluorescente utilisant une sonde spécifique de télomères et sur une étape d'interpolation utilisant une courbe d'étalonnage corrélant l'intensité de fluorescence et la longueur des télomères obtenue à partir d'une collection de lignées cellulaires de longueur de télomères connue. L'invention porte en outre sur des procédés pour l'identification de niches de cellules souches à l'intérieur de tissus et pour l'identification de composés aptes à déclencher la mobilisation de cellules souches à l'aide de la longueur des télomères comme critère pour l'identification de cellules souches et qui reposent sur la technique de cartographie télomérique de cellules individuelles de l'invention.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2008/055791 WO2009138117A1 (fr) | 2008-05-12 | 2008-05-12 | Procédés et réactifs pour la détermination de la longueur de télomères d'une manière semi-automatique de chaque cellule individuelle dans une population de cellules immobilisées |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2723950A1 CA2723950A1 (fr) | 2009-11-19 |
| CA2723950C true CA2723950C (fr) | 2016-07-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2723950A Active CA2723950C (fr) | 2008-05-12 | 2008-05-12 | Procedes et reactifs pour la determination de la longueur de telomeres d'une maniere semi-automatique de chaque cellule individuelle dans une population de cellules immobilisees |
Country Status (2)
| Country | Link |
|---|---|
| CA (1) | CA2723950C (fr) |
| WO (1) | WO2009138117A1 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106535740B (zh) | 2014-05-02 | 2019-10-15 | 马萨诸塞眼科耳科诊所 | 用于确定表示角膜染色量的分数的方法和系统 |
| JP6938489B2 (ja) * | 2015-10-23 | 2021-09-22 | ノバルティス・エイジーＮｏｖａｒｔｉｓ ＡＧ | 視野に存在する選択された細胞のバイオマーカー陽性率の値を導き出す方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5741677A (en) * | 1995-06-07 | 1998-04-21 | Geron Corporation | Methods for measuring telomere length |
| WO2004035597A2 (fr) * | 2002-10-16 | 2004-04-29 | Women And Infants Hospital Of Rhode Island, Inc. | Procede permettant d'evaluer le risque d'echec de la reproduction par mesure de la longueur d'un telomere |
| WO2008081451A2 (fr) * | 2007-01-03 | 2008-07-10 | Monaliza Medical Ltd. | Méthode et trousse d'analyse du matériel génétique d'un foetus |
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- 2008-05-12 CA CA2723950A patent/CA2723950C/fr active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009138117A1 (fr) | 2009-11-19 |
| CA2723950A1 (fr) | 2009-11-19 |
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