CA2700532A1 - Dual oligonucleotide method of nucleic acid detection - Google Patents
Dual oligonucleotide method of nucleic acid detection Download PDFInfo
- Publication number
- CA2700532A1 CA2700532A1 CA2700532A CA2700532A CA2700532A1 CA 2700532 A1 CA2700532 A1 CA 2700532A1 CA 2700532 A CA2700532 A CA 2700532A CA 2700532 A CA2700532 A CA 2700532A CA 2700532 A1 CA2700532 A1 CA 2700532A1
- Authority
- CA
- Canada
- Prior art keywords
- oligonucleotide
- nucleotide sequence
- oligonucleotides
- nucleic acid
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 238000000034 method Methods 0.000 title claims abstract description 53
- 230000009977 dual effect Effects 0.000 title description 6
- 238000001514 detection method Methods 0.000 title description 3
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Classifications
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| PCT/US2008/077831 WO2009042851A1 (en) | 2007-09-28 | 2008-09-26 | Dual oligonucleotide method of nucleic acid detection |
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| CA2700532A1 true CA2700532A1 (en) | 2009-04-02 |
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| US20110200995A1 (en) * | 2009-09-04 | 2011-08-18 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of vancomycin resistance genes and vancomycin resistant enterococci |
| WO2011116318A1 (en) * | 2010-03-19 | 2011-09-22 | The Translational Genomics Research Institute | Methods, kits, and compositions useful in selecting an antibiotic to treat mrsa |
| US9074243B2 (en) * | 2012-07-27 | 2015-07-07 | New England Biolabs, Inc. | Detection of amplification products |
| US10077475B2 (en) * | 2013-01-24 | 2018-09-18 | California Institute Of Technology | FRET-based analytes detection and related methods and systems |
| EP4570924A3 (en) | 2013-01-24 | 2025-09-03 | California Institute of Technology | Chromophore-based characterization and detection methods |
| CN103045733B (zh) * | 2013-02-05 | 2014-05-14 | 中国农业科学院油料作物研究所 | 无探针双引物标记交互荧光能量共振转移定量pcr方法 |
| GB2512631A (en) * | 2013-04-03 | 2014-10-08 | Rupert Maxwell Gaut | Quantitative detection of specific nucleic acid sequences |
| WO2018232595A1 (zh) * | 2017-06-20 | 2018-12-27 | 深圳华大智造科技有限公司 | Pcr引物对及其应用 |
| WO2018232598A1 (zh) * | 2017-06-20 | 2018-12-27 | 深圳华大智造科技有限公司 | Pcr引物对及其应用 |
| WO2018232597A1 (zh) * | 2017-06-20 | 2018-12-27 | 深圳华大智造科技有限公司 | 用于定量pcr扩增的组合物及其应用 |
| AU2020337069A1 (en) | 2019-08-28 | 2022-04-14 | Contamination Source Identification, Inc. | Rapid isolation and collection of microbial RNA from a biological specimen |
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| US3687808A (en) * | 1969-08-14 | 1972-08-29 | Univ Leland Stanford Junior | Synthetic polynucleotides |
| US4469863A (en) * | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5235033A (en) * | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
| US4996143A (en) * | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
| US5750666A (en) * | 1988-05-26 | 1998-05-12 | Competitve Technologies, Inc. | Polynucleotide phosphorodithioate compounds |
| US5683896A (en) * | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| US5035996A (en) * | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| US5596086A (en) * | 1990-09-20 | 1997-01-21 | Gilead Sciences, Inc. | Modified internucleoside linkages having one nitrogen and two carbon atoms |
| DE69231853T2 (de) * | 1991-11-07 | 2001-09-13 | Nanotronics, Inc. | Hybridisierung von mit chromophoren und fluorophoren konjugierten polynukleotiden zur erzeugung eines donor-zu-donor energietransfersystems |
| PT912766E (pt) | 1996-06-04 | 2009-07-16 | Univ Utah Res Found | Monitorização da hibridização durante a pcr |
| EP1674585B1 (en) | 1996-06-04 | 2010-10-20 | University of Utah Research Foundation | Apparatus for performing PCR and monitoring the reaction in real time during temperature cycling |
| JP3016759B2 (ja) | 1997-02-28 | 2000-03-06 | スミスクライン・ビーチャム・コーポレイション | 競争的ハイブリダイゼーションによる蛍光エネルギー転移 |
| WO1999049293A2 (en) | 1998-03-24 | 1999-09-30 | Boston Probes, Inc. | Methods, kits and compositions pertaining to detection complexes |
| US6627159B1 (en) * | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
| US6720187B2 (en) * | 2000-06-28 | 2004-04-13 | 3M Innovative Properties Company | Multi-format sample processing devices |
| US6734401B2 (en) * | 2000-06-28 | 2004-05-11 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US7192560B2 (en) * | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| US7220544B2 (en) * | 2002-05-08 | 2007-05-22 | Arkray, Inc. | Method for detecting target nucleic acid |
| CN1548545A (zh) * | 2003-05-14 | 2004-11-24 | 宇 王 | 一种用于多靶序列pcr检测的公共引物及其对多靶核酸序列进行pcr扩增的方法 |
| AU2006242201A1 (en) * | 2005-05-02 | 2006-11-09 | Stratagene California | Oligonucleotide probe/primer compositions and methods for polynucleotide detection |
| EP1907560B1 (en) * | 2005-05-20 | 2013-01-23 | Integrated DNA Technologies, Inc. | Compounds and methods for labeling oligonucleotides |
| GB0510979D0 (en) | 2005-05-28 | 2005-07-06 | Kbiosciences Ltd | Detection system for PCR assay |
| RU2005132940A (ru) * | 2006-03-01 | 2007-11-27 | Институт биохимии и генетики Уфимского научного центра РАН (RU) | Способ детекции специфических фрагментов днк или рнк с помощью полимеразной цепной реакции в режиме реального времени |
| WO2007060707A2 (en) | 2005-11-23 | 2007-05-31 | Diasorin Spa | Reagents and method for simultaneous nucleic acid amplification and detection |
| CN101017141A (zh) * | 2006-02-09 | 2007-08-15 | 港龙生物技术（深圳）有限公司 | 诊断人类乳头状病毒（hpv）感染的荧光聚合酶链式反应（pcr）的方法和试剂盒 |
| CN101033486B (zh) * | 2006-03-08 | 2011-04-06 | 缪金明 | 一种荧光定量pcr检测方法 |
| CN100475976C (zh) * | 2006-08-18 | 2009-04-08 | 上海科华生物工程股份有限公司 | 一种同步扩增检测肝炎及艾滋病毒核酸的试剂盒 |
-
2008
- 2008-09-26 CN CN200880109213.0A patent/CN101809170B/zh not_active Expired - Fee Related
- 2008-09-26 DE DE602008006254T patent/DE602008006254D1/de active Active
- 2008-09-26 AT AT08834519T patent/ATE505562T1/de not_active IP Right Cessation
- 2008-09-26 WO PCT/US2008/077831 patent/WO2009042851A1/en not_active Ceased
- 2008-09-26 JP JP2010527172A patent/JP5432154B2/ja not_active Expired - Fee Related
- 2008-09-26 BR BRPI0816047A patent/BRPI0816047A2/pt not_active IP Right Cessation
- 2008-09-26 US US12/680,049 patent/US8313931B2/en not_active Expired - Fee Related
- 2008-09-26 EP EP08834519A patent/EP2209909B1/en not_active Not-in-force
- 2008-09-26 CA CA2700532A patent/CA2700532A1/en not_active Abandoned
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2012
- 2012-11-20 US US13/681,574 patent/US8652812B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ATE505562T1 (de) | 2011-04-15 |
| CN101809170A (zh) | 2010-08-18 |
| US20130171635A1 (en) | 2013-07-04 |
| JP5432154B2 (ja) | 2014-03-05 |
| DE602008006254D1 (de) | 2011-05-26 |
| US8652812B2 (en) | 2014-02-18 |
| BRPI0816047A2 (pt) | 2018-06-05 |
| US8313931B2 (en) | 2012-11-20 |
| US20100203537A1 (en) | 2010-08-12 |
| JP2010539940A (ja) | 2010-12-24 |
| WO2009042851A1 (en) | 2009-04-02 |
| EP2209909A1 (en) | 2010-07-28 |
| EP2209909B1 (en) | 2011-04-13 |
| CN101809170B (zh) | 2014-08-20 |
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