CA2694532A1 - Method of lipooligosaccharide (los) typing of a neisseria strain comprising detection of los biosynthesis genes - Google Patents
Method of lipooligosaccharide (los) typing of a neisseria strain comprising detection of los biosynthesis genes Download PDFInfo
- Publication number
- CA2694532A1 CA2694532A1 CA2694532A CA2694532A CA2694532A1 CA 2694532 A1 CA2694532 A1 CA 2694532A1 CA 2694532 A CA2694532 A CA 2694532A CA 2694532 A CA2694532 A CA 2694532A CA 2694532 A1 CA2694532 A1 CA 2694532A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- gene
- igtg
- strain
- chromosome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 325
- 238000000034 method Methods 0.000 title claims abstract description 186
- 241000588653 Neisseria Species 0.000 title claims abstract description 28
- 230000015572 biosynthetic process Effects 0.000 title abstract description 10
- 238000001514 detection method Methods 0.000 title description 5
- 241001212279 Neisseriales Species 0.000 claims description 46
- 210000000349 chromosome Anatomy 0.000 claims description 37
- 238000006243 chemical reaction Methods 0.000 claims description 30
- 238000012408 PCR amplification Methods 0.000 claims description 23
- 102000004169 proteins and genes Human genes 0.000 claims description 23
- 238000012163 sequencing technique Methods 0.000 claims description 23
- 239000000523 sample Substances 0.000 claims description 19
- 230000003321 amplification Effects 0.000 claims description 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 13
- 238000009396 hybridization Methods 0.000 claims description 12
- 241000588650 Neisseria meningitidis Species 0.000 claims description 11
- 108020004999 messenger RNA Proteins 0.000 claims description 11
- 239000002299 complementary DNA Substances 0.000 claims description 8
- 238000003745 diagnosis Methods 0.000 claims description 8
- 238000007403 mPCR Methods 0.000 claims description 7
- 230000002759 chromosomal effect Effects 0.000 claims description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 5
- 210000003578 bacterial chromosome Anatomy 0.000 claims description 5
- 239000013592 cell lysate Substances 0.000 claims description 5
- 208000015181 infectious disease Diseases 0.000 claims description 4
- 239000012472 biological sample Substances 0.000 claims description 2
- 101100241857 Schizosaccharomyces pombe (strain 972 / ATCC 24843) oac1 gene Proteins 0.000 claims 10
- 101150038319 oac1 gene Proteins 0.000 claims 10
- 238000003757 reverse transcription PCR Methods 0.000 claims 2
- 241000588652 Neisseria gonorrhoeae Species 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 description 54
- SUHOOTKUPISOBE-UHFFFAOYSA-N O-phosphoethanolamine Chemical compound NCCOP(O)(O)=O SUHOOTKUPISOBE-UHFFFAOYSA-N 0.000 description 42
- 239000000047 product Substances 0.000 description 34
- 101100121705 Drosophila melanogaster sens gene Proteins 0.000 description 33
- 238000004458 analytical method Methods 0.000 description 30
- 108020004414 DNA Proteins 0.000 description 27
- 230000002441 reversible effect Effects 0.000 description 27
- 102000004190 Enzymes Human genes 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 16
- 239000000203 mixture Substances 0.000 description 16
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 12
- 239000012634 fragment Substances 0.000 description 11
- 238000004885 tandem mass spectrometry Methods 0.000 description 11
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Natural products NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 10
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 10
- 239000008103 glucose Substances 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 239000002773 nucleotide Substances 0.000 description 8
- 125000003729 nucleotide group Chemical group 0.000 description 8
- 239000004471 Glycine Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 150000002386 heptoses Chemical class 0.000 description 7
- 230000001105 regulatory effect Effects 0.000 description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000001962 electrophoresis Methods 0.000 description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 208000002109 Argyria Diseases 0.000 description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 5
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 5
- 239000007997 Tricine buffer Substances 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 150000002402 hexoses Chemical class 0.000 description 5
- 238000004949 mass spectrometry Methods 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- 238000001556 precipitation Methods 0.000 description 5
- 238000012340 reverse transcriptase PCR Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 208000002352 blister Diseases 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 230000014509 gene expression Effects 0.000 description 4
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 4
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 4
- 239000006166 lysate Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000001712 DNA sequencing Methods 0.000 description 3
- 238000010222 PCR analysis Methods 0.000 description 3
- 238000006640 acetylation reaction Methods 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- -1 dCTP or dATP Chemical class 0.000 description 3
- 238000005034 decoration Methods 0.000 description 3
- 239000002158 endotoxin Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 230000000951 immunodiffusion Effects 0.000 description 3
- 238000010348 incorporation Methods 0.000 description 3
- 229920006008 lipopolysaccharide Polymers 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 239000003208 petroleum Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000009450 sialylation Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- WPNJAUFVNXKLIM-UHFFFAOYSA-N Moxonidine Chemical compound COC1=NC(C)=NC(Cl)=C1NC1=NCCN1 WPNJAUFVNXKLIM-UHFFFAOYSA-N 0.000 description 2
- 238000005481 NMR spectroscopy Methods 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000001851 biosynthetic effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 101150095079 menB gene Proteins 0.000 description 2
- 101150085064 menC gene Proteins 0.000 description 2
- 208000037941 meningococcal disease Diseases 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 208000031729 Bacteremia Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 108091028026 C-DNA Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000013502 data validation Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- HWJHWSBFPPPIPD-UHFFFAOYSA-N ethoxyethane;propan-2-one Chemical compound CC(C)=O.CCOCC HWJHWSBFPPPIPD-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000030414 genetic transfer Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical class O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- IEQCXFNWPAHHQR-UHFFFAOYSA-N lacto-N-neotetraose Natural products OCC1OC(OC2C(C(OC3C(OC(O)C(O)C3O)CO)OC(CO)C2O)O)C(NC(=O)C)C(O)C1OC1OC(CO)C(O)C(O)C1O IEQCXFNWPAHHQR-UHFFFAOYSA-N 0.000 description 1
- 101150018810 lgtB gene Proteins 0.000 description 1
- GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical compound O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229940031937 polysaccharide vaccine Drugs 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 1
- 125000005629 sialic acid group Chemical group 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US95355107P | 2007-08-02 | 2007-08-02 | |
| US60/953,551 | 2007-08-02 | ||
| PCT/EP2008/060077 WO2009016237A2 (en) | 2007-08-02 | 2008-07-31 | Molecular typing of neisseria strains by determining the presence of genes involved in lipooligosaccharide (los) biosynthesis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2694532A1 true CA2694532A1 (en) | 2009-02-05 |
Family
ID=40202081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2694532A Abandoned CA2694532A1 (en) | 2007-08-02 | 2008-07-31 | Method of lipooligosaccharide (los) typing of a neisseria strain comprising detection of los biosynthesis genes |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20100285465A1 (https=) |
| EP (1) | EP2185727A2 (https=) |
| JP (1) | JP2010535019A (https=) |
| BR (1) | BRPI0814793A2 (https=) |
| CA (1) | CA2694532A1 (https=) |
| WO (1) | WO2009016237A2 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2792687A1 (en) * | 2010-03-10 | 2011-09-15 | Glaxosmithkline Biologicals S.A. | Immunogenic composition |
| CN102154466B (zh) * | 2011-01-11 | 2013-03-06 | 天津生物芯片技术有限责任公司 | 一种对脑膜炎奈瑟氏菌的its特异的核苷酸及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060057160A1 (en) * | 2002-08-02 | 2006-03-16 | Ralph Biemans | Vaccine composition |
| CA2654706A1 (en) * | 2006-06-12 | 2007-12-21 | Nathalie Devos | Neisseria meningitidis lipooligosaccharide vaccine |
| CA2700573C (en) * | 2006-09-26 | 2016-11-22 | Cedars-Sinai Medical Center | Cancer stem cell antigen vaccines and methods |
-
2008
- 2008-07-31 CA CA2694532A patent/CA2694532A1/en not_active Abandoned
- 2008-07-31 BR BRPI0814793-0A2A patent/BRPI0814793A2/pt not_active IP Right Cessation
- 2008-07-31 US US12/671,227 patent/US20100285465A1/en not_active Abandoned
- 2008-07-31 EP EP08802949A patent/EP2185727A2/en not_active Withdrawn
- 2008-07-31 JP JP2010518679A patent/JP2010535019A/ja active Pending
- 2008-07-31 WO PCT/EP2008/060077 patent/WO2009016237A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| JP2010535019A (ja) | 2010-11-18 |
| WO2009016237A2 (en) | 2009-02-05 |
| WO2009016237A3 (en) | 2009-03-26 |
| BRPI0814793A2 (pt) | 2015-02-03 |
| US20100285465A1 (en) | 2010-11-11 |
| EP2185727A2 (en) | 2010-05-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10066268B2 (en) | Methods of diagnosing or treating prostate cancer using the ERG gene, alone or in combination with other over or under expressed genes in prostate cancer | |
| Tzeng et al. | The MisR/MisS two-component regulatory system influences inner core structure and immunotype of lipooligosaccharide in Neisseria meningitidis | |
| JPWO2019159884A5 (https=) | ||
| US20040137530A1 (en) | BASB006 polypeptides from neisseria meningitidis and immunogenic compositions thereof | |
| NO326994B1 (no) | Polypeptid og fragmenter derav, polynukleotid, ekspresjonsvektor, vertscelle, fremgangsmate, vaksinesammensetning, anvendelse samt antistoff. | |
| CA2694532A1 (en) | Method of lipooligosaccharide (los) typing of a neisseria strain comprising detection of los biosynthesis genes | |
| KR20220078659A (ko) | 생물학적 샘플 및 rna 안정화를 위한 조성물, 방법 및 키트 | |
| CN119842722B (zh) | 转录因子sp1在促进fbxo44基因表达中的应用 | |
| Post et al. | Identification of genes involved in the expression of atypical lipooligosaccharide structures from a second class of Haemophilus ducreyi | |
| CN105506160A (zh) | 幽门螺杆菌定量和毒力多重基因检测体系及其试剂盒和应用 | |
| CN109022566B (zh) | 发作性运动诱发性运动障碍相关的基因突变靶点及其应用 | |
| CN102337261A (zh) | 对脑膜炎奈瑟氏菌抗原特异的核苷酸及其应用 | |
| KR101108967B1 (ko) | 스트렙토코커스 미티스 검출용 프라이머, 프로브 및 이를 이용한 스트렙토코커스 미티스의 검출방법 | |
| JP3810162B2 (ja) | 腸管出血性大腸菌Ｏ１５７の２３ＳｒＲＮＡ又はその遺伝子に含まれるプローブ用核酸断片、それらを用いる腸管出血性大腸菌Ｏ１５７の検出法及び検出用キット | |
| CN114144531A (zh) | 用于检测粪便样品中的罕见dna序列的方法 | |
| CN106701799B (zh) | 一种马铃薯m病毒基因组全序列及其应用 | |
| KR102099392B1 (ko) | 개체의 제엽염 진단용 조성물 및 키트, 개체가 제엽염에 걸렸는지를 진단하는 방법 및 제엽염 치료제를 탐색하는 방법 | |
| US9809863B2 (en) | Flavivirus associated with Theiler's disease | |
| KR100812795B1 (ko) | 탄저균 검출용 프라이머, 프로브 및 이를 이용한 탄저균의검출방법 | |
| RU2397251C1 (ru) | Набор для детекции возбудителя легионеллеза в биологическом материале и объектах окружающей среды | |
| KR100337194B1 (ko) | 내위 표준 ｒｎａ와 이를 이용한 장내바이러스성 질환의 진단방법 | |
| JP2007508029A (ja) | マイコバクテリウム・アビウム亜種パラツベルクローシスの分子レベルの検出 | |
| US20200002750A1 (en) | Methods for detecting c. canimorsus capsular serotypes in a sample | |
| CN109504757A (zh) | 检测泛素蛋白酶基因psmb1启动子多态性的方法和试剂盒 | |
| AU2011213720B2 (en) | Methods of diagnosing or treating prostate cancer using the ERG gene, alone or in combination with other over or under expressed genes in prostate cancer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |
Effective date: 20140731 |