CA2675103C - Systeme micro-reseau et son procede - Google Patents
Systeme micro-reseau et son procede Download PDFInfo
- Publication number
- CA2675103C CA2675103C CA2675103A CA2675103A CA2675103C CA 2675103 C CA2675103 C CA 2675103C CA 2675103 A CA2675103 A CA 2675103A CA 2675103 A CA2675103 A CA 2675103A CA 2675103 C CA2675103 C CA 2675103C
- Authority
- CA
- Canada
- Prior art keywords
- spot
- light
- image
- micro
- array
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000010208 microarray analysis Methods 0.000 title description 11
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- 238000003384 imaging method Methods 0.000 claims description 20
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- 244000005700 microbiome Species 0.000 abstract description 8
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- 238000011002 quantification Methods 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 8
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- 241000588724 Escherichia coli Species 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/12—Apparatus specially adapted for use in combinatorial chemistry or with libraries for screening libraries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00702—Processes involving means for analysing and characterising the products
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
La présente invention concerne un système (2) et un procédé permettant d'analyser des points dans un micro-réseau (5), les points contenant un spécimen cible. Ledit procédé comprend les étapes consistant à : disposer d'une lame recouverte d'un micro-réseau de points ; éclairer au moins l'un des points (500, 51) ; diriger la lumière sur ce point ; recueillir la lumière émise par le point ; former une image du point à l'aide de la lumière recueillie (502) ; et analyser l'image pour distinguer au moins une unité du spécimen cible contenu dans le point parmi tout matériau non désiré (506, 507). Les résultats de l'analyse permettent de détecter, classer et quantifier un spécimen à résolution optique tels des micro-organismes ou des cellules.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CA2007/000113 WO2008092226A1 (fr) | 2007-01-29 | 2007-01-29 | Système micro-réseau et son procédé |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2675103A1 CA2675103A1 (fr) | 2008-08-07 |
| CA2675103C true CA2675103C (fr) | 2013-10-01 |
Family
ID=39673585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2675103A Active CA2675103C (fr) | 2007-01-29 | 2007-01-29 | Systeme micro-reseau et son procede |
Country Status (2)
| Country | Link |
|---|---|
| CA (1) | CA2675103C (fr) |
| WO (1) | WO2008092226A1 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10359363B2 (en) | 2015-06-30 | 2019-07-23 | Imec Vzw | Time, space digitally resolved quantification of luminescent targets |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5528050A (en) * | 1995-07-24 | 1996-06-18 | Molecular Dynamics, Inc. | Compact scan head with multiple scanning modalities |
| US6297018B1 (en) * | 1998-04-17 | 2001-10-02 | Ljl Biosystems, Inc. | Methods and apparatus for detecting nucleic acid polymorphisms |
| US6674885B2 (en) * | 2002-06-04 | 2004-01-06 | Amersham Biosciences Corp | Systems and methods for analyzing target contrast features in images of biological samples |
| JP4037869B2 (ja) * | 2002-11-07 | 2008-01-23 | 富士通株式会社 | 画像解析支援方法、画像解析支援プログラムおよび画像解析支援装置 |
| US7522762B2 (en) * | 2003-04-16 | 2009-04-21 | Inverness Medical-Biostar, Inc. | Detection, resolution, and identification of arrayed elements |
-
2007
- 2007-01-29 CA CA2675103A patent/CA2675103C/fr active Active
- 2007-01-29 WO PCT/CA2007/000113 patent/WO2008092226A1/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CA2675103A1 (fr) | 2008-08-07 |
| WO2008092226A1 (fr) | 2008-08-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |