CA2655875A1 - Detection of antibiotics - Google Patents

Detection of antibiotics Download PDF

Info

Publication number
CA2655875A1
CA2655875A1 CA002655875A CA2655875A CA2655875A1 CA 2655875 A1 CA2655875 A1 CA 2655875A1 CA 002655875 A CA002655875 A CA 002655875A CA 2655875 A CA2655875 A CA 2655875A CA 2655875 A1 CA2655875 A1 CA 2655875A1
Authority
CA
Canada
Prior art keywords
antibiotic
binding protein
immobilized
labelled
antibiotics
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002655875A
Other languages
French (fr)
Other versions
CA2655875C (en
Inventor
Cornelis Jacobus Ven Der Laken
Roger Piasio
Jagdish Chander Kapur
Nicolaas Cornelis Maria Emanuel Barendse
Henry Gerard Julius Hirs
Jan Verweij
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Neogen Corp
Original Assignee
Neogen Corporation
Cornelis Jacobus Ven Der Laken
Roger Piasio
Jagdish Chander Kapur
Nicolaas Cornelis Maria Emanuel Barendse
Henry Gerard Julius Hirs
Jan Verweij
Gist - Brocades N.V.
Dsm N.V.
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neogen Corporation, Cornelis Jacobus Ven Der Laken, Roger Piasio, Jagdish Chander Kapur, Nicolaas Cornelis Maria Emanuel Barendse, Henry Gerard Julius Hirs, Jan Verweij, Gist - Brocades N.V., Dsm N.V., Dsm Ip Assets B.V. filed Critical Neogen Corporation
Publication of CA2655875A1 publication Critical patent/CA2655875A1/en
Application granted granted Critical
Publication of CA2655875C publication Critical patent/CA2655875C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A process for the detection of antibiotics in a liquid medium such as milk, urine and blood is disclosed which comprises - bringing together a fluid sample of the liquid medium, an labelled antibiotic binding protein, and an immobilized antibiotic, - allowing the labelled antibiotic binding protein to bind with the immobilized antibiotic, - removing labelled antibiotic binding protein which is not bound to immobilized antibiotic, and - determining the amount of the labelled antibiotic binding protein bound to the immobilized antibiotic.

Claims (3)

1. Antibiotic-BSA conjugate selected from the group consisting of 7ACA-spacer-BSA and N-pentadecakis [N-(4-carbonyl-3-methylceph-3-em-7-yl)amino-carbonylethyldithioethylcarbonyl]BSA.
2. The conjugate according to claim 1, wherein the conjugate is 7ACA-spacer-BSA.
3. The conjugate according to claim 1, wherein the conjugate is N-pentadecakis [N-(4-carbonyl-3-methylceph-3-em-7-yl)amino-carbonylethyldithioethylcarbonyl]BSA, wherein the conjugate is N-pentadecakis [N-(4-carbonyl-3-em-7-yl) amino-carbonylethyldithioethylcarbonyl] BSA.
CA2655875A 1992-10-06 1993-10-06 Detection of antibiotics Expired - Lifetime CA2655875C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP92203084 1992-10-06
EP92203084.6 1992-10-06
CA 2107856 CA2107856C (en) 1992-10-06 1993-10-06 Detection of antibiotics

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA 2107856 Division CA2107856C (en) 1992-10-06 1993-10-06 Detection of antibiotics

Publications (2)

Publication Number Publication Date
CA2655875A1 true CA2655875A1 (en) 1994-04-07
CA2655875C CA2655875C (en) 2010-07-20

Family

ID=8210952

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2655875A Expired - Lifetime CA2655875C (en) 1992-10-06 1993-10-06 Detection of antibiotics
CA 2107856 Expired - Lifetime CA2107856C (en) 1992-10-06 1993-10-06 Detection of antibiotics

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA 2107856 Expired - Lifetime CA2107856C (en) 1992-10-06 1993-10-06 Detection of antibiotics

Country Status (6)

Country Link
JP (1) JPH06207936A (en)
AT (1) ATE170292T1 (en)
AU (1) AU662656B2 (en)
CA (2) CA2655875C (en)
DE (1) DE69320583T2 (en)
NZ (1) NZ248864A (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3368681T3 (en) * 2015-11-20 2019-08-12 Centrient Pharmaceuticals Netherlands B V ANALYSIS FOR DETERMINING ANTIBIOTICS IN WASTE
JP2021189080A (en) * 2020-06-02 2021-12-13 公立大学法人福島県立医科大学 Method for immobilizing compound on substrate and method for detecting immobilized compound

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4239852A (en) * 1978-06-12 1980-12-16 Penicillin Assays, Inc. Antibiotic detection method
US4347312A (en) * 1980-03-20 1982-08-31 Research Triangle Institute Detection of antibiotics in milk

Also Published As

Publication number Publication date
ATE170292T1 (en) 1998-09-15
DE69320583D1 (en) 1998-10-01
CA2107856A1 (en) 1994-04-07
AU4879293A (en) 1994-04-21
CA2107856C (en) 2009-04-28
AU662656B2 (en) 1995-09-07
CA2655875C (en) 2010-07-20
DE69320583T2 (en) 1999-01-14
JPH06207936A (en) 1994-07-26
NZ248864A (en) 1994-10-26

Similar Documents

Publication Publication Date Title
DE69835893T2 (en) DIAGNOSTIC TESTS AND KITS FOR CLOSTRIDIUM DIFFICILE
WO1994001775A1 (en) Immunoassay using dye complexed enzyme conjugates
EP0397113A3 (en) Method of detecting specific binding substances in body fluids
CA2224674A1 (en) Immunoassay and kit with two reagents that are cross-linked if they adhere to an analyte
CA2254108A1 (en) Improvements in or relating to specific binding assays
CA2201153A1 (en) Monoclonal antibody to human cardiac myoglobin
WO1991012023A3 (en) Hybrid reagents capable of selectively releasing molecules into cells
EP0325045A3 (en) Immunoassay method for detecting antigens
CA2259421A1 (en) Labelling and selection of molecules
EP1376130A3 (en) Assay device with two opposable members
WO1995018826A3 (en) Purified primate ctla-8 antigens and related reagents
EP0279097A3 (en) Immunoassay test strip
EP0747703A3 (en) Method for analyzing biological active substances
AU3598289A (en) Visual discrimination qualitative enzyme assay
AU591636B2 (en) Assay method employing fluorescent cell incorporative dye
CA2151142A1 (en) Glycoprotein ligand for p-selectin and methods of use thereof
CA2140794A1 (en) Assay for the determination of protein in a biological sample
CA2179823A1 (en) Method of assay using two distinguishable types of particles
WO2020260716A3 (en) Substrate, kit system and method for biological assays
CA2007336A1 (en) Diagnostic test using chimeric antibodies
EP0402846A3 (en) Fluorescence polarization immunoassay for tetrahydrocannabinoids
CA2193323A1 (en) Process for preparing a synthetic calibrator for use in sandwich immunoassays, which calibrator consists of an antibody against one of the antibodies used in the assay and of a sequence of the analyte
EP0363109A3 (en) Determination of a chlamydial or gonococcal antigen using a positively-charged ionically binding support
CA1268115A (en) Method and composition for detecting analyte moieties
EP1742056A3 (en) Determination of a specific immunglobulin using multiple antigens

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20131007