CA2645507A1 - Vaccine for viruses that cause persistent or latent infections - Google Patents
Vaccine for viruses that cause persistent or latent infections Download PDFInfo
- Publication number
- CA2645507A1 CA2645507A1 CA 2645507 CA2645507A CA2645507A1 CA 2645507 A1 CA2645507 A1 CA 2645507A1 CA 2645507 CA2645507 CA 2645507 CA 2645507 A CA2645507 A CA 2645507A CA 2645507 A1 CA2645507 A1 CA 2645507A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- virus
- mcmv
- mice
- infection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000700605 Viruses Species 0.000 title claims abstract description 172
- 230000002085 persistent effect Effects 0.000 title claims abstract description 20
- 208000032420 Latent Infection Diseases 0.000 title claims abstract description 19
- 229960005486 vaccine Drugs 0.000 title claims description 134
- 208000037581 Persistent Infection Diseases 0.000 title abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 79
- 241000701022 Cytomegalovirus Species 0.000 claims abstract description 31
- 239000000203 mixture Substances 0.000 claims abstract description 30
- 241001529453 unidentified herpesvirus Species 0.000 claims abstract description 16
- 208000006454 hepatitis Diseases 0.000 claims abstract description 8
- 231100000283 hepatitis Toxicity 0.000 claims abstract description 8
- 241001631646 Papillomaviridae Species 0.000 claims abstract description 7
- 241001430294 unidentified retrovirus Species 0.000 claims abstract description 7
- 108020004414 DNA Proteins 0.000 claims description 296
- 108090000623 proteins and genes Proteins 0.000 claims description 171
- 239000013612 plasmid Substances 0.000 claims description 120
- 241000701024 Human betaherpesvirus 5 Species 0.000 claims description 97
- 230000005867 T cell response Effects 0.000 claims description 49
- 230000002238 attenuated effect Effects 0.000 claims description 43
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 25
- 108700005077 Viral Genes Proteins 0.000 claims description 23
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 22
- 102000053602 DNA Human genes 0.000 claims description 18
- 206010011831 Cytomegalovirus infection Diseases 0.000 claims description 16
- 210000000056 organ Anatomy 0.000 claims description 15
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 claims description 11
- 101150009795 UL54 gene Proteins 0.000 claims description 10
- 208000037771 disease arising from reactivation of latent virus Diseases 0.000 claims description 9
- 230000009385 viral infection Effects 0.000 claims description 7
- 208000036142 Viral infection Diseases 0.000 claims description 6
- 230000002779 inactivation Effects 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 208000015181 infectious disease Diseases 0.000 abstract description 149
- 238000011282 treatment Methods 0.000 abstract description 12
- 230000002265 prevention Effects 0.000 abstract description 6
- 241000699670 Mus sp. Species 0.000 description 225
- 230000003053 immunization Effects 0.000 description 163
- 230000004224 protection Effects 0.000 description 162
- 238000002649 immunization Methods 0.000 description 157
- 230000001681 protective effect Effects 0.000 description 138
- 241000701074 Human alphaherpesvirus 2 Species 0.000 description 127
- 210000001744 T-lymphocyte Anatomy 0.000 description 119
- 230000004044 response Effects 0.000 description 99
- 108091007433 antigens Proteins 0.000 description 77
- 102000036639 antigens Human genes 0.000 description 77
- 210000004027 cell Anatomy 0.000 description 77
- 239000013598 vector Substances 0.000 description 77
- 239000000427 antigen Substances 0.000 description 75
- 238000007912 intraperitoneal administration Methods 0.000 description 73
- 102000004169 proteins and genes Human genes 0.000 description 69
- 229940037003 alum Drugs 0.000 description 62
- 230000003612 virological effect Effects 0.000 description 60
- 238000002474 experimental method Methods 0.000 description 53
- 230000036039 immunity Effects 0.000 description 53
- 210000000952 spleen Anatomy 0.000 description 51
- 238000003556 assay Methods 0.000 description 45
- 108700039887 Essential Genes Proteins 0.000 description 41
- 241000700584 Simplexvirus Species 0.000 description 41
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 41
- 201000010099 disease Diseases 0.000 description 40
- 210000001266 CD8-positive T-lymphocyte Anatomy 0.000 description 39
- 241000700198 Cavia Species 0.000 description 35
- 241000699666 Mus <mouse, genus> Species 0.000 description 34
- 210000004988 splenocyte Anatomy 0.000 description 34
- 230000003472 neutralizing effect Effects 0.000 description 33
- 108090000765 processed proteins & peptides Proteins 0.000 description 33
- 238000011725 BALB/c mouse Methods 0.000 description 32
- 241000701029 Murid betaherpesvirus 1 Species 0.000 description 32
- 229940021995 DNA vaccine Drugs 0.000 description 31
- 108010041986 DNA Vaccines Proteins 0.000 description 30
- 230000014509 gene expression Effects 0.000 description 30
- 230000029812 viral genome replication Effects 0.000 description 30
- 238000002255 vaccination Methods 0.000 description 29
- 230000001404 mediated effect Effects 0.000 description 28
- 230000037452 priming Effects 0.000 description 27
- 239000000047 product Substances 0.000 description 27
- 230000009885 systemic effect Effects 0.000 description 27
- 102000003886 Glycoproteins Human genes 0.000 description 26
- 108090000288 Glycoproteins Proteins 0.000 description 26
- 101710110377 Immediate early protein IE1 Proteins 0.000 description 26
- 101710205424 Immediate-early protein 1 Proteins 0.000 description 26
- 108700026244 Open Reading Frames Proteins 0.000 description 26
- 230000028993 immune response Effects 0.000 description 25
- 210000004072 lung Anatomy 0.000 description 25
- 239000002953 phosphate buffered saline Substances 0.000 description 25
- 210000003079 salivary gland Anatomy 0.000 description 25
- 238000012360 testing method Methods 0.000 description 25
- 230000001154 acute effect Effects 0.000 description 22
- 239000002671 adjuvant Substances 0.000 description 22
- 238000002347 injection Methods 0.000 description 21
- 239000007924 injection Substances 0.000 description 21
- 230000009467 reduction Effects 0.000 description 21
- 210000001519 tissue Anatomy 0.000 description 19
- 230000003902 lesion Effects 0.000 description 17
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical group CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 16
- 108020005202 Viral DNA Proteins 0.000 description 16
- 238000004458 analytical method Methods 0.000 description 16
- 238000013459 approach Methods 0.000 description 16
- 238000001727 in vivo Methods 0.000 description 15
- 208000035415 Reinfection Diseases 0.000 description 14
- 150000001413 amino acids Chemical class 0.000 description 14
- 210000004443 dendritic cell Anatomy 0.000 description 14
- 230000007774 longterm Effects 0.000 description 14
- 230000010076 replication Effects 0.000 description 14
- 208000001688 Herpes Genitalis Diseases 0.000 description 13
- 230000005875 antibody response Effects 0.000 description 13
- 201000004946 genital herpes Diseases 0.000 description 13
- 230000001965 increasing effect Effects 0.000 description 13
- 241000700588 Human alphaherpesvirus 1 Species 0.000 description 12
- 231100000111 LD50 Toxicity 0.000 description 12
- 238000010171 animal model Methods 0.000 description 12
- 230000000890 antigenic effect Effects 0.000 description 12
- 238000011554 guinea pig model Methods 0.000 description 12
- 102000004196 processed proteins & peptides Human genes 0.000 description 12
- 230000000306 recurrent effect Effects 0.000 description 12
- 230000000638 stimulation Effects 0.000 description 12
- 238000001514 detection method Methods 0.000 description 11
- 238000011161 development Methods 0.000 description 11
- 230000002458 infectious effect Effects 0.000 description 11
- 210000004185 liver Anatomy 0.000 description 11
- 238000012552 review Methods 0.000 description 11
- 241001183012 Modified Vaccinia Ankara virus Species 0.000 description 10
- 101100195065 Murid herpesvirus 1 (strain Smith) RIR1 gene Proteins 0.000 description 10
- 241000700618 Vaccinia virus Species 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 238000000338 in vitro Methods 0.000 description 10
- 150000003839 salts Chemical class 0.000 description 10
- 230000001954 sterilising effect Effects 0.000 description 10
- 210000002845 virion Anatomy 0.000 description 10
- 102000043129 MHC class I family Human genes 0.000 description 9
- 108091054437 MHC class I family Proteins 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 9
- 108091036078 conserved sequence Proteins 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 230000007246 mechanism Effects 0.000 description 9
- 238000010172 mouse model Methods 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 230000002829 reductive effect Effects 0.000 description 9
- 230000007485 viral shedding Effects 0.000 description 9
- 238000011740 C57BL/6 mouse Methods 0.000 description 8
- 102000009016 Cholera Toxin Human genes 0.000 description 8
- 108010049048 Cholera Toxin Proteins 0.000 description 8
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 8
- 241000282412 Homo Species 0.000 description 8
- 108060004795 Methyltransferase Proteins 0.000 description 8
- 230000024932 T cell mediated immunity Effects 0.000 description 8
- 108010067390 Viral Proteins Proteins 0.000 description 8
- 230000000840 anti-viral effect Effects 0.000 description 8
- 230000005847 immunogenicity Effects 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 8
- 238000010186 staining Methods 0.000 description 8
- 102100022717 Atypical chemokine receptor 1 Human genes 0.000 description 7
- 230000003682 DNA packaging effect Effects 0.000 description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 7
- 238000002965 ELISA Methods 0.000 description 7
- 208000009889 Herpes Simplex Diseases 0.000 description 7
- 101000678879 Homo sapiens Atypical chemokine receptor 1 Proteins 0.000 description 7
- 108010065805 Interleukin-12 Proteins 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000009977 dual effect Effects 0.000 description 7
- 102000054766 genetic haplotypes Human genes 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 239000013600 plasmid vector Substances 0.000 description 7
- 230000000069 prophylactic effect Effects 0.000 description 7
- 230000007420 reactivation Effects 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 241000700199 Cavia porcellus Species 0.000 description 6
- 230000004543 DNA replication Effects 0.000 description 6
- 238000011238 DNA vaccination Methods 0.000 description 6
- -1 ICPO Proteins 0.000 description 6
- 102000013462 Interleukin-12 Human genes 0.000 description 6
- 230000003190 augmentative effect Effects 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 210000000609 ganglia Anatomy 0.000 description 6
- 210000004392 genitalia Anatomy 0.000 description 6
- 230000003993 interaction Effects 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- 229940031626 subunit vaccine Drugs 0.000 description 6
- 230000014616 translation Effects 0.000 description 6
- 238000013519 translation Methods 0.000 description 6
- 210000003501 vero cell Anatomy 0.000 description 6
- 208000021075 Creatine deficiency syndrome Diseases 0.000 description 5
- 101150026402 DBP gene Proteins 0.000 description 5
- 108010002350 Interleukin-2 Proteins 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000007969 cellular immunity Effects 0.000 description 5
- 201000008609 cerebral creatine deficiency syndrome Diseases 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 238000002716 delivery method Methods 0.000 description 5
- 230000001419 dependent effect Effects 0.000 description 5
- 238000000684 flow cytometry Methods 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 230000002163 immunogen Effects 0.000 description 5
- 231100000518 lethal Toxicity 0.000 description 5
- 230000001665 lethal effect Effects 0.000 description 5
- 210000002540 macrophage Anatomy 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000013642 negative control Substances 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 238000011002 quantification Methods 0.000 description 5
- 230000002195 synergetic effect Effects 0.000 description 5
- 238000013518 transcription Methods 0.000 description 5
- 230000035897 transcription Effects 0.000 description 5
- 238000001262 western blot Methods 0.000 description 5
- 102000004127 Cytokines Human genes 0.000 description 4
- 108090000695 Cytokines Proteins 0.000 description 4
- 102000016559 DNA Primase Human genes 0.000 description 4
- 108010092681 DNA Primase Proteins 0.000 description 4
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 4
- 108010074328 Interferon-gamma Proteins 0.000 description 4
- 101710125418 Major capsid protein Proteins 0.000 description 4
- 241001529936 Murinae Species 0.000 description 4
- 108010089430 Phosphoproteins Proteins 0.000 description 4
- 102000007982 Phosphoproteins Human genes 0.000 description 4
- 101150056567 UL70 gene Proteins 0.000 description 4
- 241000711975 Vesicular stomatitis virus Species 0.000 description 4
- 108010003533 Viral Envelope Proteins Proteins 0.000 description 4
- 210000000612 antigen-presenting cell Anatomy 0.000 description 4
- 230000003416 augmentation Effects 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 4
- KQNZDYYTLMIZCT-KQPMLPITSA-N brefeldin A Chemical compound O[C@@H]1\C=C\C(=O)O[C@@H](C)CCC\C=C\[C@@H]2C[C@H](O)C[C@H]21 KQNZDYYTLMIZCT-KQPMLPITSA-N 0.000 description 4
- JUMGSHROWPPKFX-UHFFFAOYSA-N brefeldin-A Natural products CC1CCCC=CC2(C)CC(O)CC2(C)C(O)C=CC(=O)O1 JUMGSHROWPPKFX-UHFFFAOYSA-N 0.000 description 4
- 230000036755 cellular response Effects 0.000 description 4
- 238000012790 confirmation Methods 0.000 description 4
- 230000034994 death Effects 0.000 description 4
- 231100000517 death Toxicity 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 230000017188 evasion or tolerance of host immune response Effects 0.000 description 4
- 230000001900 immune effect Effects 0.000 description 4
- 230000015788 innate immune response Effects 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 4
- 239000011859 microparticle Substances 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 210000000822 natural killer cell Anatomy 0.000 description 4
- 230000008506 pathogenesis Effects 0.000 description 4
- 230000003362 replicative effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 230000014599 transmission of virus Effects 0.000 description 4
- 210000004291 uterus Anatomy 0.000 description 4
- 210000001215 vagina Anatomy 0.000 description 4
- 230000007442 viral DNA synthesis Effects 0.000 description 4
- 101150010802 CVC2 gene Proteins 0.000 description 3
- 102000019034 Chemokines Human genes 0.000 description 3
- 108010012236 Chemokines Proteins 0.000 description 3
- 101710094648 Coat protein Proteins 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 3
- 208000022555 Genital disease Diseases 0.000 description 3
- 241000701068 Human herpesvirus 2 strain 333 Species 0.000 description 3
- 102100037850 Interferon gamma Human genes 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 3
- 101710151805 Mitochondrial intermediate peptidase 1 Proteins 0.000 description 3
- 101710151803 Mitochondrial intermediate peptidase 2 Proteins 0.000 description 3
- 206010065764 Mucosal infection Diseases 0.000 description 3
- 101150098384 NEC2 gene Proteins 0.000 description 3
- 238000011529 RT qPCR Methods 0.000 description 3
- 101150110861 TRM2 gene Proteins 0.000 description 3
- 101150048584 TRM3 gene Proteins 0.000 description 3
- 102100026890 Tumor necrosis factor ligand superfamily member 4 Human genes 0.000 description 3
- 206010053613 Type IV hypersensitivity reaction Diseases 0.000 description 3
- 101150011902 UL52 gene Proteins 0.000 description 3
- 230000033289 adaptive immune response Effects 0.000 description 3
- 238000000540 analysis of variance Methods 0.000 description 3
- 230000030741 antigen processing and presentation Effects 0.000 description 3
- 230000014102 antigen processing and presentation of exogenous peptide antigen via MHC class I Effects 0.000 description 3
- 238000000376 autoradiography Methods 0.000 description 3
- 210000001185 bone marrow Anatomy 0.000 description 3
- 239000013592 cell lysate Substances 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000012636 effector Substances 0.000 description 3
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 210000003754 fetus Anatomy 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 101150029683 gB gene Proteins 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 230000000977 initiatory effect Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000006166 lysate Substances 0.000 description 3
- 101710130522 mRNA export factor Proteins 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 210000004779 membrane envelope Anatomy 0.000 description 3
- 229940035032 monophosphoryl lipid a Drugs 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000003389 potentiating effect Effects 0.000 description 3
- 230000000644 propagated effect Effects 0.000 description 3
- 238000011321 prophylaxis Methods 0.000 description 3
- 102000005962 receptors Human genes 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000003393 splenic effect Effects 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 3
- 230000032258 transport Effects 0.000 description 3
- 230000005951 type IV hypersensitivity Effects 0.000 description 3
- 208000027930 type IV hypersensitivity disease Diseases 0.000 description 3
- 241000701161 unidentified adenovirus Species 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 2
- 108010029697 CD40 Ligand Proteins 0.000 description 2
- 102100032937 CD40 ligand Human genes 0.000 description 2
- 101150072608 CVC1 gene Proteins 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- 241001227713 Chiron Species 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 description 2
- 101150040913 DUT gene Proteins 0.000 description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 2
- 101150013191 E gene Proteins 0.000 description 2
- 101710121417 Envelope glycoprotein Proteins 0.000 description 2
- 206010015150 Erythema Diseases 0.000 description 2
- 206010073931 Genital herpes simplex Diseases 0.000 description 2
- 206010061978 Genital lesion Diseases 0.000 description 2
- 108060003393 Granulin Proteins 0.000 description 2
- 241000725303 Human immunodeficiency virus Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CPELXLSAUQHCOX-UHFFFAOYSA-N Hydrogen bromide Chemical compound Br CPELXLSAUQHCOX-UHFFFAOYSA-N 0.000 description 2
- 101150102264 IE gene Proteins 0.000 description 2
- 206010060708 Induration Diseases 0.000 description 2
- 102100034349 Integrase Human genes 0.000 description 2
- 108090000172 Interleukin-15 Proteins 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 108090000942 Lactalbumin Proteins 0.000 description 2
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 2
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 2
- 108091061960 Naked DNA Proteins 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 230000006819 RNA synthesis Effects 0.000 description 2
- 102000000505 Ribonucleotide Reductases Human genes 0.000 description 2
- 108010041388 Ribonucleotide Reductases Proteins 0.000 description 2
- 241000702670 Rotavirus Species 0.000 description 2
- 101000999689 Saimiriine herpesvirus 2 (strain 11) Transcriptional regulator ICP22 homolog Proteins 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 101150102071 TRX1 gene Proteins 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 102100040247 Tumor necrosis factor Human genes 0.000 description 2
- 101150004957 UL25 gene Proteins 0.000 description 2
- 101150104684 UL44 gene Proteins 0.000 description 2
- 101150117989 UL46 gene Proteins 0.000 description 2
- 101150053996 UL47 gene Proteins 0.000 description 2
- 101150063032 UL51 gene Proteins 0.000 description 2
- 101150022492 UL83 gene Proteins 0.000 description 2
- 206010046865 Vaccinia virus infection Diseases 0.000 description 2
- 201000008100 Vaginitis Diseases 0.000 description 2
- 108010087302 Viral Structural Proteins Proteins 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000006907 apoptotic process Effects 0.000 description 2
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 2
- SRSXLGNVWSONIS-UHFFFAOYSA-M benzenesulfonate Chemical compound [O-]S(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-M 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 239000013553 cell monolayer Substances 0.000 description 2
- 108091092356 cellular DNA Proteins 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 108010021994 cytomegalovirus matrix protein 65kDa Proteins 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 229950005627 embonate Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 101150002378 gC gene Proteins 0.000 description 2
- 229940050410 gluconate Drugs 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000011597 hartley guinea pig Methods 0.000 description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000003308 immunostimulating effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 150000007529 inorganic bases Chemical class 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 101150066555 lacZ gene Proteins 0.000 description 2
- 238000010150 least significant difference test Methods 0.000 description 2
- 230000021633 leukocyte mediated immunity Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 210000001165 lymph node Anatomy 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 201000004792 malaria Diseases 0.000 description 2
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- PSGAAPLEWMOORI-PEINSRQWSA-N medroxyprogesterone acetate Chemical compound C([C@@]12C)CC(=O)C=C1[C@@H](C)C[C@@H]1[C@@H]2CC[C@]2(C)[C@@](OC(C)=O)(C(C)=O)CC[C@H]21 PSGAAPLEWMOORI-PEINSRQWSA-N 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 230000000869 mutational effect Effects 0.000 description 2
- 230000000926 neurological effect Effects 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 229940068196 placebo Drugs 0.000 description 2
- 239000000902 placebo Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 229940023867 prime-boost vaccine Drugs 0.000 description 2
- 229940021993 prophylactic vaccine Drugs 0.000 description 2
- 238000002331 protein detection Methods 0.000 description 2
- 238000001243 protein synthesis Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 description 2
- 229960001860 salicylate Drugs 0.000 description 2
- 239000012723 sample buffer Substances 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 2
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 229940095064 tartrate Drugs 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 230000010474 transient expression Effects 0.000 description 2
- 238000003146 transient transfection Methods 0.000 description 2
- 229940125575 vaccine candidate Drugs 0.000 description 2
- 208000007089 vaccinia Diseases 0.000 description 2
- 239000003981 vehicle Substances 0.000 description 2
- 230000006648 viral gene expression Effects 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- FFEARJCKVFRZRR-ZLINWBGDSA-N (2S)-2-amino-4-methyl(33S)sulfanylbutanoic acid Chemical compound N[C@@H](CC[33S]C)C(=O)O FFEARJCKVFRZRR-ZLINWBGDSA-N 0.000 description 1
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 1
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-M 3-carboxy-2,3-dihydroxypropanoate Chemical compound OC(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-M 0.000 description 1
- ALKYHXVLJMQRLQ-UHFFFAOYSA-M 3-carboxynaphthalen-2-olate Chemical compound C1=CC=C2C=C(C([O-])=O)C(O)=CC2=C1 ALKYHXVLJMQRLQ-UHFFFAOYSA-M 0.000 description 1
- 101150096316 5 gene Proteins 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000269627 Amphiuma means Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 108700016947 Bos taurus structural-GP Proteins 0.000 description 1
- 102000001902 CC Chemokines Human genes 0.000 description 1
- 108010040471 CC Chemokines Proteins 0.000 description 1
- 108050006947 CXC Chemokine Proteins 0.000 description 1
- 102000019388 CXC chemokine Human genes 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010055166 Chemokine CCL5 Proteins 0.000 description 1
- 102000001327 Chemokine CCL5 Human genes 0.000 description 1
- 102000016951 Chemokine CXCL2 Human genes 0.000 description 1
- 108010014414 Chemokine CXCL2 Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010010430 Congenital cytomegalovirus infection Diseases 0.000 description 1
- 206010062343 Congenital infection Diseases 0.000 description 1
- 229940046168 CpG oligodeoxynucleotide Drugs 0.000 description 1
- 101710197003 DNA polymerase catalytic subunit Proteins 0.000 description 1
- 230000007018 DNA scission Effects 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 1
- 101710096438 DNA-binding protein Proteins 0.000 description 1
- 206010011891 Deafness neurosensory Diseases 0.000 description 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101710146739 Enterotoxin Proteins 0.000 description 1
- 101710126487 Envelope glycoprotein B Proteins 0.000 description 1
- 101710204837 Envelope small membrane protein Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 238000000729 Fisher's exact test Methods 0.000 description 1
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 1
- 101710177291 Gag polyprotein Proteins 0.000 description 1
- 206010048461 Genital infection Diseases 0.000 description 1
- 101150109586 Gk gene Proteins 0.000 description 1
- 229940124841 Herpesvirus vaccine Drugs 0.000 description 1
- 101000767151 Homo sapiens General vesicular transport factor p115 Proteins 0.000 description 1
- 101001111288 Homo sapiens NFU1 iron-sulfur cluster scaffold homolog, mitochondrial Proteins 0.000 description 1
- 101001093919 Homo sapiens SEC14-like protein 2 Proteins 0.000 description 1
- 101000802734 Homo sapiens eIF5-mimic protein 2 Proteins 0.000 description 1
- 241000701085 Human alphaherpesvirus 3 Species 0.000 description 1
- 101100427508 Human cytomegalovirus (strain AD169) UL39 gene Proteins 0.000 description 1
- 101000714193 Human cytomegalovirus Tegument protein Proteins 0.000 description 1
- 108700008569 Human cytomegalovirus oriLyt Proteins 0.000 description 1
- 101001042049 Human herpesvirus 1 (strain 17) Transcriptional regulator ICP22 Proteins 0.000 description 1
- 101900102284 Human herpesvirus 1 ICP47 protein Proteins 0.000 description 1
- 101000999690 Human herpesvirus 2 (strain HG52) E3 ubiquitin ligase ICP22 Proteins 0.000 description 1
- 101900228213 Human herpesvirus 2 Envelope glycoprotein D Proteins 0.000 description 1
- 101150027427 ICP4 gene Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108010007403 Immediate-Early Proteins Proteins 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000006992 Interferon-alpha Human genes 0.000 description 1
- 108010047761 Interferon-alpha Proteins 0.000 description 1
- 102000004890 Interleukin-8 Human genes 0.000 description 1
- 108090001007 Interleukin-8 Proteins 0.000 description 1
- 101150062031 L gene Proteins 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 241000222732 Leishmania major Species 0.000 description 1
- 108010028921 Lipopeptides Proteins 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 101710145006 Lysis protein Proteins 0.000 description 1
- 102000043131 MHC class II family Human genes 0.000 description 1
- 108091054438 MHC class II family Proteins 0.000 description 1
- 102000009571 Macrophage Inflammatory Proteins Human genes 0.000 description 1
- 108010009474 Macrophage Inflammatory Proteins Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 101710177509 Major immediate early protein Proteins 0.000 description 1
- 101000767152 Mus musculus General vesicular transport factor p115 Proteins 0.000 description 1
- 101001093920 Mus musculus SEC14-like protein 2 Proteins 0.000 description 1
- 241001467552 Mycobacterium bovis BCG Species 0.000 description 1
- 101150041636 NEC1 gene Proteins 0.000 description 1
- 102100024011 NFU1 iron-sulfur cluster scaffold homolog, mitochondrial Human genes 0.000 description 1
- 108091008877 NK cell receptors Proteins 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 102000010648 Natural Killer Cell Receptors Human genes 0.000 description 1
- 208000020241 Neonatal disease Diseases 0.000 description 1
- 208000037129 Newborn Diseases Infant Diseases 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241000191474 Northern cereal mosaic cytorhabdovirus Species 0.000 description 1
- 108700001237 Nucleic Acid-Based Vaccines Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 241000223960 Plasmodium falciparum Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 101710186352 Probable membrane antigen 3 Proteins 0.000 description 1
- 101710181078 Probable membrane antigen 75 Proteins 0.000 description 1
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 description 1
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 description 1
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 102000002067 Protein Subunits Human genes 0.000 description 1
- 102000012515 Protein kinase domains Human genes 0.000 description 1
- 108050002122 Protein kinase domains Proteins 0.000 description 1
- 101150093191 RIR1 gene Proteins 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 206010067470 Rotavirus infection Diseases 0.000 description 1
- 239000012722 SDS sample buffer Substances 0.000 description 1
- 208000009966 Sensorineural Hearing Loss Diseases 0.000 description 1
- 241000713311 Simian immunodeficiency virus Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000037453 T cell priming Effects 0.000 description 1
- 210000000662 T-lymphocyte subset Anatomy 0.000 description 1
- 229920002253 Tannate Polymers 0.000 description 1
- 101710178472 Tegument protein Proteins 0.000 description 1
- 210000000447 Th1 cell Anatomy 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 101800000385 Transmembrane protein Proteins 0.000 description 1
- 206010060872 Transplant failure Diseases 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 101150104047 UL17 gene Proteins 0.000 description 1
- 101150003230 UL27 gene Proteins 0.000 description 1
- 101150008036 UL29 gene Proteins 0.000 description 1
- 101150068034 UL30 gene Proteins 0.000 description 1
- 101150081727 UL32 gene Proteins 0.000 description 1
- 101150017804 UL33 gene Proteins 0.000 description 1
- 101150087430 UL34 gene Proteins 0.000 description 1
- 101150099321 UL42 gene Proteins 0.000 description 1
- 101150066971 UL49 gene Proteins 0.000 description 1
- 101150081415 UL55 gene Proteins 0.000 description 1
- 101150015312 UL56 gene Proteins 0.000 description 1
- 101150095805 UL7 gene Proteins 0.000 description 1
- 101150113240 UL71 gene Proteins 0.000 description 1
- 101150016730 UL79 gene Proteins 0.000 description 1
- 101150088910 UL82 gene Proteins 0.000 description 1
- 101150041688 UL87 gene Proteins 0.000 description 1
- 101150104254 UL92 gene Proteins 0.000 description 1
- 101150074007 UL99 gene Proteins 0.000 description 1
- 241000587120 Vaccinia virus Ankara Species 0.000 description 1
- 206010046914 Vaginal infection Diseases 0.000 description 1
- 208000032159 Vaginal inflammation Diseases 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 230000010530 Virus Neutralization Effects 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004721 adaptive immunity Effects 0.000 description 1
- 230000002187 allostimulatory effect Effects 0.000 description 1
- 230000002424 anti-apoptotic effect Effects 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 229940121357 antivirals Drugs 0.000 description 1
- XTKDAFGWCDAMPY-UHFFFAOYSA-N azaperone Chemical compound C1=CC(F)=CC=C1C(=O)CCCN1CCN(C=2N=CC=CC=2)CC1 XTKDAFGWCDAMPY-UHFFFAOYSA-N 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 229940077388 benzenesulfonate Drugs 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000010322 bone marrow transplantation Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000010410 calcium alginate Nutrition 0.000 description 1
- 239000000648 calcium alginate Substances 0.000 description 1
- 229960002681 calcium alginate Drugs 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
- 229940030156 cell vaccine Drugs 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 208000015114 central nervous system disease Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 201000004709 chorioretinitis Diseases 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 108700013218 cytomegalovirus m152 Proteins 0.000 description 1
- 108700010903 cytomegalovirus proteins Proteins 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 229940063223 depo-provera Drugs 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- ACYGYJFTZSAZKR-UHFFFAOYSA-J dicalcium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Ca+2].[Ca+2].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O ACYGYJFTZSAZKR-UHFFFAOYSA-J 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000001177 diphosphate Substances 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 102100035859 eIF5-mimic protein 2 Human genes 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 229940009662 edetate Drugs 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000147 enterotoxin Substances 0.000 description 1
- 231100000655 enterotoxin Toxicity 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 230000009842 establishment of viral latency Effects 0.000 description 1
- 229950000206 estolate Drugs 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229940050411 fumarate Drugs 0.000 description 1
- 230000009760 functional impairment Effects 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000030414 genetic transfer Effects 0.000 description 1
- 229960001731 gluceptate Drugs 0.000 description 1
- KWMLJOLKUYYJFJ-VFUOTHLCSA-N glucoheptonic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O)C(O)=O KWMLJOLKUYYJFJ-VFUOTHLCSA-N 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 229940049906 glutamate Drugs 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 244000038280 herbivores Species 0.000 description 1
- 230000005745 host immune response Effects 0.000 description 1
- 230000007236 host immunity Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- XGIHQYAWBCFNPY-AZOCGYLKSA-N hydrabamine Chemical compound C([C@@H]12)CC3=CC(C(C)C)=CC=C3[C@@]2(C)CCC[C@@]1(C)CNCCNC[C@@]1(C)[C@@H]2CCC3=CC(C(C)C)=CC=C3[C@@]2(C)CCC1 XGIHQYAWBCFNPY-AZOCGYLKSA-N 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000037451 immune surveillance Effects 0.000 description 1
- 230000003611 immunoevasive effect Effects 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 238000010324 immunological assay Methods 0.000 description 1
- 230000002480 immunoprotective effect Effects 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229940031551 inactivated vaccine Drugs 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940117681 interleukin-12 Drugs 0.000 description 1
- 230000019734 interleukin-12 production Effects 0.000 description 1
- 229940096397 interleukin-8 Drugs 0.000 description 1
- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 description 1
- 238000010212 intracellular staining Methods 0.000 description 1
- SUMDYPCJJOFFON-UHFFFAOYSA-N isethionic acid Chemical compound OCCS(O)(=O)=O SUMDYPCJJOFFON-UHFFFAOYSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229940001447 lactate Drugs 0.000 description 1
- 229940099584 lactobionate Drugs 0.000 description 1
- JYTUSYBCFIZPBE-AMTLMPIISA-N lactobionic acid Chemical compound OC(=O)[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O JYTUSYBCFIZPBE-AMTLMPIISA-N 0.000 description 1
- 210000001821 langerhans cell Anatomy 0.000 description 1
- 108010034897 lentil lectin Proteins 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 229940124590 live attenuated vaccine Drugs 0.000 description 1
- 229940023012 live-attenuated vaccine Drugs 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 238000002803 maceration Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229940049920 malate Drugs 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- IWYDHOAUDWTVEP-UHFFFAOYSA-M mandelate Chemical compound [O-]C(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-M 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000005541 medical transmission Effects 0.000 description 1
- 229960002985 medroxyprogesterone acetate Drugs 0.000 description 1
- 238000002941 microtiter virus yield reduction assay Methods 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- OHDXDNUPVVYWOV-UHFFFAOYSA-N n-methyl-1-(2-naphthalen-1-ylsulfanylphenyl)methanamine Chemical compound CNCC1=CC=CC=C1SC1=CC=CC2=CC=CC=C12 OHDXDNUPVVYWOV-UHFFFAOYSA-N 0.000 description 1
- 230000004719 natural immunity Effects 0.000 description 1
- 238000007857 nested PCR Methods 0.000 description 1
- 229940023146 nucleic acid vaccine Drugs 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 208000001749 optic atrophy Diseases 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000007530 organic bases Chemical class 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229940014662 pantothenate Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 230000000803 paradoxical effect Effects 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 210000003899 penis Anatomy 0.000 description 1
- 229940023041 peptide vaccine Drugs 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 238000009520 phase I clinical trial Methods 0.000 description 1
- 238000002962 plaque-reduction assay Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000010149 post-hoc-test Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 229940023143 protein vaccine Drugs 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 125000001308 pyruvoyl group Chemical group O=C([*])C(=O)C([H])([H])[H] 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 210000005000 reproductive tract Anatomy 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 150000003335 secondary amines Chemical class 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 231100000879 sensorineural hearing loss Toxicity 0.000 description 1
- 208000023573 sensorineural hearing loss disease Diseases 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000005582 sexual transmission Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000012453 sprague-dawley rat model Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229950002757 teoclate Drugs 0.000 description 1
- 108010040614 terminase Proteins 0.000 description 1
- 150000003512 tertiary amines Chemical class 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229940021747 therapeutic vaccine Drugs 0.000 description 1
- 210000004906 toe nail Anatomy 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 101150046896 trm1 gene Proteins 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 239000012646 vaccine adjuvant Substances 0.000 description 1
- 230000005570 vertical transmission Effects 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000007733 viral latency Effects 0.000 description 1
- 230000008957 viral persistence Effects 0.000 description 1
- 230000007419 viral reactivation Effects 0.000 description 1
- 229960004854 viral vaccine Drugs 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 239000012130 whole-cell lysate Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/245—Herpetoviridae, e.g. herpes simplex virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
- A61P31/22—Antivirals for DNA viruses for herpes viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5252—Virus inactivated (killed)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5254—Virus avirulent or attenuated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/53—DNA (RNA) vaccination
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
- A61K2039/541—Mucosal route
- A61K2039/543—Mucosal route intranasal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/545—Medicinal preparations containing antigens or antibodies characterised by the dose, timing or administration schedule
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55505—Inorganic adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55544—Bacterial toxins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55561—CpG containing adjuvants; Oligonucleotide containing adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
- C12N2710/16634—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Epidemiology (AREA)
- Oncology (AREA)
- Engineering & Computer Science (AREA)
- Communicable Diseases (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US78112306P | 2006-03-10 | 2006-03-10 | |
| US60/781,123 | 2006-03-10 | ||
| PCT/US2007/006113 WO2007106404A2 (en) | 2006-03-10 | 2007-03-09 | Vaccine for viruses that cause persistent or latent infections |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2645507A1 true CA2645507A1 (en) | 2007-09-20 |
Family
ID=38510006
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2645507 Abandoned CA2645507A1 (en) | 2006-03-10 | 2007-03-09 | Vaccine for viruses that cause persistent or latent infections |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8501194B2 (https=) |
| EP (1) | EP2001516A4 (https=) |
| JP (1) | JP2009529576A (https=) |
| CA (1) | CA2645507A1 (https=) |
| WO (1) | WO2007106404A2 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009037359A1 (en) * | 2007-09-21 | 2009-03-26 | Sanofi Pasteur | Vaccine composition for the prevention of cmv infections |
| EP3756684A1 (en) | 2009-05-22 | 2020-12-30 | Genocea Biosciences, Inc. | Vaccines against herpes simplex virus type 2: compositions and methods for eliciting an immune response |
| US8979890B2 (en) | 2010-10-01 | 2015-03-17 | Ethicon Endo-Surgery, Inc. | Surgical instrument with jaw member |
| JP6055776B2 (ja) | 2010-11-24 | 2016-12-27 | ジェノセア バイオサイエンシーズ， インコーポレイテッド | 単純ヘルペスウイルス２型に対するワクチン：免疫応答を誘発する組成物及び方法 |
| EA033467B1 (ru) * | 2011-01-31 | 2019-10-31 | Univ Pennsylvania | Молекула нуклеиновой кислоты для индукции иммунного ответа против герпесвируса и ее применение |
| EP2726097A4 (en) * | 2011-07-01 | 2015-03-11 | Univ California | HERPES VIRUS VACCINE AND METHOD OF USE |
| US20140348863A1 (en) * | 2011-10-12 | 2014-11-27 | Alessia Bianchi | Cmv antigens and uses thereof |
| AU2012340712B2 (en) | 2011-11-23 | 2017-09-14 | Genocea Biosciences, Inc. | Nucleic acid vaccines against Herpes Simplex Virus type 2: compositions and methods for eliciting an immune response |
| EP3246400B1 (en) * | 2012-01-09 | 2019-10-23 | Sanofi Pasteur Biologics, LLC | Purification of herpes virus |
| EP2852662B1 (en) | 2012-05-21 | 2020-09-16 | Sanofi Pasteur Limited | Herpesvirus compositions and related methods |
| WO2014005643A1 (en) * | 2012-07-05 | 2014-01-09 | Okairos Ag | Novel prime-boosting regimens involving immunogenic polypeptides encoded by polynucleotides |
| CA2890061A1 (en) * | 2012-12-04 | 2014-06-12 | Merck Sharp & Dohme Corp. | Conditional replicating viral vectors |
| MX384992B (es) | 2014-06-13 | 2025-03-14 | Glaxosmithkline Biologicals Sa | Combinaciones inmunógenas. |
| US10314383B2 (en) | 2015-03-30 | 2019-06-11 | Gary McClellan Thompson | Wearable sports equipment carrier with personal flotation device |
| US10611800B2 (en) | 2016-03-11 | 2020-04-07 | Pfizer Inc. | Human cytomegalovirus gB polypeptide |
| US10350288B2 (en) | 2016-09-28 | 2019-07-16 | Genocea Biosciences, Inc. | Methods and compositions for treating herpes |
| CN111032681B (zh) * | 2017-08-30 | 2024-05-17 | Km生物医薬股份公司 | 修饰型HSV gB蛋白及包含其的HSV疫苗 |
| MA53158A (fr) * | 2018-07-20 | 2021-05-26 | Aicuris Gmbh & Co Kg | Procédés de criblage et d'identification d'agents inhibant ou modulant le complexe de sortie nucléaire du virus de l'herpès |
| US11629172B2 (en) | 2018-12-21 | 2023-04-18 | Pfizer Inc. | Human cytomegalovirus gB polypeptide |
| TWI810589B (zh) | 2020-06-21 | 2023-08-01 | 美商輝瑞股份有限公司 | 人巨細胞病毒糖蛋白Ｂ（ｇＢ）多肽 |
| CA3230564A1 (en) * | 2021-09-13 | 2023-03-16 | Jean-Pol DETIFFE | Method to generate personalized neoantigens of a tumor of a patient |
| EP4147712A1 (en) * | 2021-09-13 | 2023-03-15 | OncoDNA | Method to generate a double-stranded dna pool encoding neoantigens of a tumor of a patient |
| US20250319132A1 (en) * | 2024-04-16 | 2025-10-16 | Mirror Biologics, Inc. | Methods and compositions for improving the immune response against viral pathogens |
-
2007
- 2007-03-09 CA CA 2645507 patent/CA2645507A1/en not_active Abandoned
- 2007-03-09 WO PCT/US2007/006113 patent/WO2007106404A2/en not_active Ceased
- 2007-03-09 EP EP07752789A patent/EP2001516A4/en not_active Withdrawn
- 2007-03-09 US US12/282,123 patent/US8501194B2/en not_active Expired - Fee Related
- 2007-03-09 JP JP2009500402A patent/JP2009529576A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2001516A4 (en) | 2010-04-28 |
| US8501194B2 (en) | 2013-08-06 |
| WO2007106404A3 (en) | 2008-03-20 |
| JP2009529576A (ja) | 2009-08-20 |
| US20100272752A1 (en) | 2010-10-28 |
| EP2001516A2 (en) | 2008-12-17 |
| WO2007106404A2 (en) | 2007-09-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8501194B2 (en) | Vaccine for viruses that cause persistent or latent infections | |
| Koelle et al. | Recent progress in herpes simplex virus immunobiology and vaccine research | |
| US9408905B2 (en) | Herpes simplex virus vaccines | |
| EP2753364B1 (en) | A conditional replicating cytomegalovirus as a vaccine for cmv | |
| US9492533B2 (en) | HSV-1 and HSV-2 vaccines and methods of use thereof | |
| JP6109165B2 (ja) | ヘルペスウイルスワクチンおよび使用方法 | |
| CA2158148A1 (en) | Defective mutant non-retroviral virus (e.g. hsv) as vaccine | |
| Chung et al. | The ongoing pursuit of a prophylactic HSV vaccine | |
| Krause et al. | Herpesvirus vaccines: development, controversies, and applications | |
| Reszka et al. | Construction and properties of a herpes simplex virus 2 dl5-29 vaccine candidate strain encoding an HSV-1 virion host shutoff protein | |
| US11390650B2 (en) | Recombinant Herpes Simplex Virus-2 expressing glycoprotein B and D antigens | |
| Brans et al. | Prevention of genital herpes simplex virus type 1 and 2 disease in mice immunized with a gD-expressing dominant-negative recombinant HSV-1 | |
| Ďurmanová et al. | Herpes Simplex Virus 1 and 2 Vaccine Design: What can | |
| HK1175807B (en) | Herpes simplex virus vaccines | |
| Bernstein | 26 Herpes Simplex Virus Vaccines | |
| HK1194283B (en) | A conditional replicating cytomegalovirus as a vaccine for cmv | |
| HK1194283A (en) | A conditional replicating cytomegalovirus as a vaccine for cmv | |
| HK1240107A1 (en) | A conditional replicating cytomegalovirus as a vaccine for cmv |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20130311 |