CA2642575C - Quantification d'adn cellulaire et de cellules au moyen d'elements de marquage - Google Patents

Quantification d'adn cellulaire et de cellules au moyen d'elements de marquage Download PDF

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Publication number
CA2642575C
CA2642575C CA2642575A CA2642575A CA2642575C CA 2642575 C CA2642575 C CA 2642575C CA 2642575 A CA2642575 A CA 2642575A CA 2642575 A CA2642575 A CA 2642575A CA 2642575 C CA2642575 C CA 2642575C
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Prior art keywords
cells
dna
sample
cell
intercalator
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Active
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CA2642575A
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CA2642575A1 (fr
Inventor
Olga Ornatsky
Vladimir Baranov
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Standard Biotools Canada Inc
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Fluidigm Canada Inc
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Publication of CA2642575A1 publication Critical patent/CA2642575A1/fr
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

La présente invention concerne des procédés et des trousses destinés à la quantification d'ADN cellulaire et de cellules. La prise passive d'éléments, des intercalants d'ADN marqués par lesdits éléments, et des réactifs d'affinité marqués par lesdits éléments sont utilisés pour quantifier l'ADN et les cellules. L'ADN et les cellules sont analysés à l'aide d'une analyse élémentaire, incluant l'ICP-MS (spectrométrie de masse à torche plasma). Les procédés et les trousses permettent une analyse rapide et précise de l'ADN cellulaire et du dénombrement des cellules.
CA2642575A 2006-02-13 2007-02-13 Quantification d'adn cellulaire et de cellules au moyen d'elements de marquage Active CA2642575C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US77258906P 2006-02-13 2006-02-13
US60/772,589 2006-02-13
PCT/CA2007/000221 WO2007140571A1 (fr) 2006-02-13 2007-02-13 Quantification d'adn cellulaire et de cellules au moyen d'éléments de marquage

Publications (2)

Publication Number Publication Date
CA2642575A1 CA2642575A1 (fr) 2007-12-13
CA2642575C true CA2642575C (fr) 2015-01-20

Family

ID=38800988

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2642575A Active CA2642575C (fr) 2006-02-13 2007-02-13 Quantification d'adn cellulaire et de cellules au moyen d'elements de marquage

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CA (1) CA2642575C (fr)
WO (1) WO2007140571A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1991694T3 (da) 2006-02-13 2016-08-01 Fluidigm Canada Inc Genekspressionsanalyse med grundstoftagget oligonukleotid
CA2658787C (fr) 2006-08-15 2013-04-09 Alexei Antonov Appareil et procede pour l'analyse elementaire de particules par spectrometrie de masse
US9218949B2 (en) 2013-06-04 2015-12-22 Fluidigm Canada, Inc. Strategic dynamic range control for time-of-flight mass spectrometry
WO2020235424A1 (fr) * 2019-05-17 2020-11-26 株式会社島津製作所 Procédé de détection de vésicules extracellulaires
CN112710829A (zh) * 2020-12-18 2021-04-27 北京清分稳同科技有限公司 一种新型冠状病毒IgG抗体的元素质谱法检测试剂盒

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4585736A (en) * 1983-10-18 1986-04-29 The United States Of America As Represented By The United States Department Of Energy Flow cytometric measurement of total DNA and incorporated halodeoxyuridine
US7479630B2 (en) * 2004-03-25 2009-01-20 Bandura Dmitry R Method and apparatus for flow cytometry linked with elemental analysis

Also Published As

Publication number Publication date
WO2007140571A1 (fr) 2007-12-13
CA2642575A1 (fr) 2007-12-13

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